Anti- V2 produced in rabbit, affinity isolated antibody

Catalog Number V5514

Product Description Ligand: arginine vasopressin Anti-Vasopressin V2 Receptor is produced in rabbit using as immunogen a synthetic peptide conjugated to Reagent KLH. The peptide corresponds to the N-terminus of the Supplied as a solution of 1 mg/ml in phosphate buffered extracellular domain of human vasopressin V2 saline containing 0.1% sodium azide as a preservative. Receptor. The antibody is affinity-purified using the immunizing peptide immobilized on agarose. Precautions and Disclaimer This product is for R&D use only, not for drug, Anti-Vasopressin V2 Receptor specifically recognizes household, or other uses. Please consult the Material human vasopressin V2 Receptor by Safety Data Sheet for information regarding hazards immunohistochemistry with formalin-fixed, paraffin- and safe handling practices. embedded tissues. Not tested for other uses. Storage/Stability The human AVPR2 (arginine 2) For continuous use, store at 2-8 °C for up to one month. locus encodes vasopressin receptor type 2, a member For extended storage, freeze in working aliquots. of the vasopressin/ family subfamily. V2 Repeated freezing and thawing, or storage in "frost- receptor has been shown to concentrate the urine and free" freezers, is not recommended. If slight turbidity maintain by responding to the occurs upon prolonged storage, clarify the solution by pituitary hormone arginine vasopressin (AVP). Loss of centrifugation before use. Working dilution samples gene function results in the disease Nephrogenic should be discarded if not used within 12 hours. Diabetes Insipidus (NDI). When extrarenal V2 receptors are stimulated by infusion of a V2 selective agonist Product Profile (dDAVP), a variety of clotting factors are released into Immunohistochemistry: a minimum working the bloodstream. The physiologic importance of this concentration of 3 mg/ml is determined using human property is not known; its absence does not appear to tissue. be detrimental in NDI patients. Note: In order to obtain the best results and assay Expression: The V2 receptor is expressed in the sensitivity in different techniques and preparations, we tubule, predominantly in the distal convoluted tubule recommend determining optimal working dilutions by and collecting ducts. The V2 Receptor is also titration test. expressed outside the kidney although its tissue localization is uncertain. AVPR2 expression has also This product manufactured by MBL International. been described in brain, breast, fetal tissue and associated with alternative splicing. ESTs IDC,PHC 05/12-2 (Expressed Sequence Tags) have been isolated from normal lung and pancreatic cancer libraries.

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