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Reef biodiversity research at the Florida Museum of Natural History Gustav Paulay, John Slapcinsky, Mandy Bemis, Sarah McPherson, Machel Malay, Sea McKeon, Francois Michonneau, John Starmer, Jada White Florida Museum of Natural History, University of Florida, Gainesville FL 32611-7800 USA, http://www.flmnh.ufl.edu/malacology/

BIOTAS – SW Indian Ocean

100 FPMAR112-08|BFLA-210|M ithrax hispidus ABSTRACT BIODIVERSITY SURVEYS 26 FPMAR104-08|BFLA-202|M ithrax hispidus FPMAR124-08|BFLA-219|Pitho mirabilis 8 100 FPM AR095-08|BFLA-197|Pitho mirabilis FPMAR216-08|BFLA-321|Epialtis bituber... Collaborative surveys aimed at documenting patterns of connectivity and speciation in SW 1 100 FPMAR215-08|BFLA-319|Epialtis bituber... FPMAR107-08|BFLA-206|Mithrac ulus cory phe

100 FPMAR103-08|BFLA-201|Mithraculus forceps Marine research at FLMNH-IZ is focused on large-scale reef biodiversity 13 FPMAR101-08|BFLA-198|Mithraculus forceps 0 Ree f bio divers ity is a ma jor focus a t FLMNH-IZ, th e larges t inver te bra te co llecti on in 87 FPM AR119-08|BFLA-217|Mithraculus forc eps FPMAR102-08|BFLA-200|Mithraculus forceps 100 90 FPMAR117-08|BFLA-215|Mithrax hispidus Indian Ocean at the scale of biotas. We are collecting, photographing, and sequencing ~1000 FPMAR178-08|BFLA-257|Paguridae FPM AR118-08|BFLA-216|Panopeus oc ciden... 13 0 FPMAR113-08|BFLA-211|Xanthodius denti... 28 surveys and on the biogeography and evolution of invertebrates. 100 FPMAR105-08|BFLA-203|Xanthodius denti... FPMAR175-08|BFLA-253|Leander tenuicornis the SE US, especially through collaborative, large-scale biodiversity surveys. 100 20 FPMAR174-08|BFLA-255|Leander tenuicornis FPMAR219-08|BFLA-328|Dardanus per locality (see Bruggemann et al talk and abstract). 100 FPMAR123-08|BFLA-225|Petrolisthes car... 00 45 FPMAR091-08|BFLA-101|Petrolisthes gal... FPMAR122-08|BFLA-220|Petrolisthes gal... Major recent surveys are outlined below; most geared toward 7 FPMAR120-08|BFLA-218|Pilum nus pannosus FPMAR116-08|BFLA-214|Pilumnus Typical surveys voucher, photo-document, and genetically characterize 1000+/- 64 100 FPMAR110-08|BFLA-208|Pilumnus

100 FPMAR114-08|BFLA-212|Pachy cheles FPMAR217-08|BFLA-320|Porcellanidae

00 FPMAR177-08|BFLA-254| FPMAR071-08|BFLA-174| hispidus 16 documenting as many species as possible with voucher specimens 100 FPMAR070-08|BFLA-173|Stenopus hispidus FPM AR183-08|BFLA-266|Latreutes fucorum species. An effort to genetically barcode 25,000 specimens of 71 FPM AR176-08|BFLA-263|Latreutes fucorum 100 FPM AR195-08|BFLA-271|Latreutes fucorum Marine Biodiversity Survey of Oman FPMAR196-08|BFLA-269|Latreutes fucorum FPMAR138-08|BFLA-249|Dissodacty lus m e... 0 100 FPMAR182-08|BFLA-273| 0 99 FPMAR181-08|BFLA-275|Portunus (with data accessible on the web), photos, and genetic samples. 0 from the collection just started. Curation, databasing, and identification is pursued FPMAR150-08|BFLA-256|Portunus gibbesii FPMAR106-08|BFLA-205|Leptodius 0 14 FPM AR111-08|BFLA-209|Menippe m ercenaria Collaborative effort to document and create guide to marine macrobiota of the Sultanate of 0 FPMAR149-08|BFLA-258|Portunus sayi 19 FPMAR115-08|BFLA-213|Pseudom edaeus ag...

100 FPM AR148-08|BFLA-248|Decapoda 8 14 FPMAR139-08|BFLA-251|Dissodactylus me... with students, staff, and a large network of specialists. 440,000 records are now FPM AR132-08|BFLA-240|Stomatopoda 0 FPMAR098-08|BFLA-168|Proc essidae FPMAR068-08|BFLA-171|Ly smata wurdem anni 1 Oman. Past efforts have been on vouchering and photo-documentation, with sequencing now FPM AR093-08|BFLA-135|Periclimenaeus 3 14 FPMAR084-08|BFLA-184|Alpheus packardii 100 FPMAR069-08|BFLA-172|Alpheus packardii 2 FPMAR097-08|BFLA-141|Calcinus tibicen FPM AR151-08|BFLA-259|Callinectes bouc... searchable online, with large photo-library soon to follow. Our goal is to make reef 1 10 FPMAR096-08|BFLA-140|Clibanarius tric... 40 FPMAR222-08|BFLA-326|Amphipoda beginning as well. 28 FPMAR169-08|BFLA-262|Amphipoda FPMAR086-08|BFLA-186|Amphipoda

100 FPMAR087-08|BFLA-187|Bem los unic ornis FPMAR085-08|BFLA-185|Bemlos unicornis 20 invertebrate diversity as well documented and accessible to all as possible. All are 100 FPMAR186-08|BFLA-280|Amphipoda 41 FPM AR074-08|BFLA-151|Caprellidae 14 15 FPM AR041-08|BFLA-146L|Amphipoda FPMAR226-08|BFLA-345|Isopoda 1 70 FPMAR034-08|BFLA-146E|Amphipoda 48 FPMAR094-08|BFLA-134|Ampithoe 9 FPMAR187-08|BFLA-281|Amphipoda FPMAR035-08|BFLA-146F|Amphipoda invited to visit, pursue research, and collaborate with these efforts. 67 6 FPMAR033-08|BFLA-146D|Amphipoda FPMAR036-08|BFLA-146G|Am phipoda Florida Invertebrate Survey FPM AR039-08|BFLA-146J|Amphipoda 100 FPM AR038-08|BFLA-146I|Amphipoda 0 FPMAR037-08|BFLA-146H|Amphipoda 67 FPMAR032-08|BFLA-146C|Amphipoda FPMAR030-08|BFLA-146A|Amphipoda FPMAR164-08|BFLA-234c |Sagitta FPMAR163-08|BFLA-234b|Sagitta 99 A yet informal , collaborative survey to voucher, photo-document, and genetically barcode 100 FPM AR162-08|BFLA-234a|Sagitta FPMAR145-08|BFLA-247B|Calanoida

76 FPM AR143-08|BFLA-246|Calanoida 88 FPM AR136-08|BFLA-243|Calanoida

48 FPMAR137-08|BFLA-244|Calanoida 0 63 FPM AR125-08|BFLA-238A|Calanoida 100 FPMAR128-08|BFLA-239|Calanoida marine invertebrates in Florida. Following years of unfocused collecting, the effort was kicked INTRODUCTION 85 FPMAR126-08|BFLA-238B|Calanoida 100 FPMAR193-08|BFLA-286|Enc ope m ichelini

100 FPMAR192-08|BFLA-288|Encope michelini FPMAR191-08|BFLA-285|M ellita isometra 47 100 FPMAR188-08|BFLA-283|M ellita isometra FPMAR241-08|BFLA-231|Calliost oma y uca... 100 FPMAR202-08|BFLA-305|Lis som yema mellita off in a 3 day workshop at SMS Ft. Pierce in 2008 that netted 250 species and tested 14 FPMAR160-08|BFLA-196|Lis som yema mellita FLMNH is Florida’s state museum and part of the University of Florida, located in 3 FPMAR067-08|BFLA-170|Alpheus formosus 100 FPMAR227-08|BFLA-346|Polychaeta 62 FPMAR218-08|BFLA-325|Polychaeta

5 FPMAR220-08|BFLA-329|Eunicidae 3 FPMAR066-08|BFLA-169|Hes ionidae FPMAR200-08|BFLA-290|Podarke obscura 19 databasing and extraction methods (tree on left) 100 FPMAR197-08|BFLA-289|Podarke obscura

100 FPMAR009-08|BFLA-106|Branchiom ma Gainesville, north-central Florida. FLMNH and adjacent FSCA (Florida State 54 9 FPMAR008-08|BFLA-109b|Terebellidae FPMAR223-08|BFLA-330|Polychaeta

6 FPMAR028-08|BFLA-133|Phragmatopoma la... 100 FPMAR018-08|BFLA-122|Phragmatopoma la...

2 100 FPMAR055-08|BFLA-158|Solemya occident... FPMAR054-08|BFLA-157|Solemya occident...

58 FPMAR156-08|BFLA-178|Chloeia viridis Collection of ) hold ~33 million specimens, about the same as the 100 15 FPMAR017-08|BFLA-120|Ophiotrix FPMAR155-08|BFLA-177|Chloeia viridis FPMAR015-08|BFLA-116|Eurythoe complanata 50 100 FPMAR014-08|BFLA-117|Eurythoe complanata 43 FPMAR158-08|BFLA-182|Hermodice c arunc ... PHYLOGENETICS, PHYLOGEOGRAPHY, BARCODING 100 FPMAR010-08|BFLA-110|Eurythoe com planata American Museum of Natural History. FLMNH is young (1891), with the bulk of the 65 FPMAR011-08|BFLA-108|Herm odic e carunc... 100 FPM AR237-08|BFLA-221|Ac anthopleura gr... 18 FPM AR051-08|BFLA-145|Ac anthopleura gr... 11 FPMAR228-08|BFLA-350|Acic ulata 5 FPM AR141-08|BFLA-237B|Calanoida FPMAR209-08|BFLA-317|Balanus amphitrite Much research is focused on marine speciation, connectivity, coevolution, and invertebrate Field and lab work 100 FPM AR208-08|BFLA-316|Balanus am phitrite 100 FPMAR294-08|BFLA-276|Litiopa m elanostom a collection acquired in the past 50 years. FLMNH-IZ (Malacology and Marine 39 FPM AR247-08|BFLA-278|Litiopa m elanostom a

11 FPMAR027-08|BFLA-130|Nass arius albus FPMAR052-08|BFLA-144|Granoturris pres... 0 2 55 FPM AR233-08|BFLA-192|Kurtziella perryae during surveys FPMAR232-08|BFLA-191|Alaba incerta phylogenetics, using sequence data. Recent biodiversity surveys include efforts to sequence 3 9 FPMAR026-08|BFLA-128|Polinices lac teus Invertebrates) expanded from mollusks to all invertebrates in 2000, and holds 28 FPMAR005-08|BFLA-104|Pus ula pedic ulus FPMAR201-08|BFLA-296|Octopus joubini

100 FPMAR025-08|BFLA-127|Olivella floralia 5 56 FPMAR023-08|BFLA-125|Olivella floralia FPMAR056-08|BFLA-136|Olivella n. sp. (left); COI FPM AR252-08|BFLA-298|Crepidula fornic ata 100 most species collected, while Sloan Foundation funding is allowing sequencing of 25,000 54 FPMAR210-08|BFLA-293|Syllidae ~440,000 databased specimen lots of >3 million specimens, in ~20 phyla. Mollusks 2 38 FPMAR254-08|BFLA-300|Crepidula plana FPMAR272-08|BFLA-339|Pollia tincta 100 FPMAR270-08|BFLA-337|Pollia tincta

100 FPMAR274-08|BFLA-341|Trachy pollia nod... 1 phylogeny of a 26 FPMAR271-08|BFLA-338|Trachypollia nod... FPMAR231-08|BFLA-190|Seila adamsi specimens from past efforts. 2 FPMAR249-08|BFLA-292|Strom bus alatus 100 FPM AR121-08|BFLA-224|Batrachonotus fr... compppgrise 90%, with other reef invertebrates most of the remainder. Rapid growth 36 FPMAR0808|058-08|BFLA-138|S uturoglypta iontha 1 87 FPMAR283-08|BFLA-355|Cos toanachis ser... sample of species 100 FPMAR281-08|BFLA-353|Costoanac his s er...

100 FPMAR282-08|BFLA-354|Haminoea antillarum 46 FPMAR280-08|BFLA-352|Haminoea antillarum 38 FPMAR065-08|BFLA-166|Bulla oc cidentalis resulted from incorporation of relinquished collections (e.g., Miami University, 64 FPMAR061-08|BFLA-143|Haminoea elegans FPM AR029-08|BFLA-131|Aplysia juliana FPMAR288-08|BFLA-360|Turbonilla abrupta collected at Fort 95 79 FPMAR287-08|BFLA-359|Turbonilla abrupta

100 FPMAR060-08|BFLA-142|Geitodoris pus ae 1 36 57 FPM AR019-08|BFLA-121|Geitodoris pus ae FPMAR057-08|BFLA-137|Pleurobranc haea ... Tulane University & University of Alabama mollusk collections, Forbush, Keeler & 32 FPM AR053-08|BFLA-150|Elysia subornata FPMAR004-08|BFLA-113|Phidiana lync eus Pierce FL (right) 39 FPMAR258-08|BFLA-304|Siphonaria pecti... 56 100 FPMAR063-08|BFLA-164|Siphonaria pecti... FPMAR045-08|BFLA-148A|Meioceras nitidum FPMAR047-08|BFLA-148C|Meioceras nitidum 100 97 FPMAR046-08|BFLA-148B|Meioceras nitidum McGinty private collections), and active field programs. 10 100 FPM AR199-08|BFLA-297B|Ostrac oda FPMAR198-08|BFLA-297A|Os tracoda

21 FPMAR211-08|BFLA-318|Branchiostoma vi... FPMAR161-08|BFLA-226|Cinachy rella kue... 30 FPMAR153-08|BFLA-180|Anthozoa 100 FPMAR221-08|BFLA-327|Actiniaria 100 FPM AR170-08|BFLA-230|Anthopleura 22 86 100 FPM AR167-08|BFLA-232|Anthopleura

100 FPM AR185-08|BFLA-277|Sc rupocellaria FPM AR184-08|BFLA-279|Sc rupocellaria

9 FPMAR006-08|BFLA-114|Lucapina suffus a 70 FPM AR242-08|BFLA-233|Diodora cayenens is 99 100 FPMAR062-08|BFLA-163|Diodora c ayenens is FPMAR064-08|BFLA-165|Antalis antillarum FPMAR263-08|BFLA-315|Chione pubera 56

57 FPMAR234-08|BFLA-193|Laevicardium pictum 100 FPM AR276-08|BFLA-347|Laevic ardium ser...

61 FPMAR275-08|BFLA-342|Arcopsis adam si FPMAR262-08|BFLA-314|Noetia ponderosa 92 FPMAR269-08|BFLA-336|Barbatia candida 100 FPMAR267-08|BFLA-334|Barbatia candida CURATION FPMAR016-08|BFLA-115|Lim a caribaea FPMAR256-08|BFLA-302|Pinctada imbricata Marine Biodiversity Survey of Guam and the Marianas 0.05 Curation and databasing Brought together records of 5640 marine species (see attached volume – available here Work on speciation usually includes comprehensive geographic and phylogenetic sampling. Calcinus latens species complex: make information from Arabian form (left), widespread form (middle), Hawaiian form (right). biodiversity surveys useful or from [email protected]), about a third were photo-documented (see URL in and accessible , and are title).100% REVISIONARY SYSTEMATICS 1981 2002 central to the operation of Biodiversity information hinges on quality of underlying . Systematic revisions using 80% FLMNH-IZ. We curate and Increasing integrative techniques are essential for reliable taxonomy and a major focus at FLMNH-IZ. database incoming 60% knowledge through collections from biodiversity large-scale surveys: surveys within 12 months. 40% proportion of species known from Guam in FLMNH-IZ serves the 20% 1981 checklist vs. second largest number of after surveys 0% invertebrate records in the Curation of incoming collection (above); a a a Algae Echiura Forams Anomura Actiniaria Sipuncula

search interface for FLMNH-IZ (below) Polychaeta Scleractinia world online. A dedicated Hydrocorals Octocorallia hinodermat rosobranchi isthobranchi cc PP E tissue collection and photo- Op database are underway. Informatics efforts to link Census of Coral Reefs - Hawaii these and survey CReefs is a collaborative survey to characterize (voucher, database, photo-document, NSF-PEET effort is databases are under barcode) reef biodiversity, with first effort focused on French Frigate Shoals, NW focused at revising development. Hawaiian Islands National Monument, where over 1000 species were documented. See reef-associated sea Moffitt et al’s talk and abstract. cucumbers, see Michonneau et al talk & abstract BioCode – French Polynesia Use TRAINING BioCode’ s objective is to document and The information content of collections is extracted througgggph use. Engage in this public resource As a university affiliated museum, genetically characterize “all” species on online, through loans, or come and visit us! FLMNH offers excellent opportunities Moorea. The marine invertebrate component for training. Undergraduates, aims to voucher, database, photo-document, Acknowledgements graduate students, postdocs and and sequence ~5000 species of invertebrates. Curation has been supported by many students and technicians over the years and funding visitors are involved in field work, Preliminary field work in 2006 netted ~1000 from NSF, State of Florida, and the McGinty Foundation. Biodiversity and sequencing surveys curation, and pursue specimen-based species (http://biocode.berkeley.edu/); Putative new species of hermit crab and have benefited greatly from participation and identification efforts of many, and funding by the research (left). current surveys start this fall. anemone (left one) from CReefs Moore Foundation, Sloan Foundation, NSF, NCI, NOAA, DOI, DOD, SeaGrant, ANR-France, Photo above courtesy of the Northwestern Hawaiian Islands Marine National Monument, Hawaiian Islands National Wildlife Refuge, the Northwestern Hawaiian Islands State Marine Refuge, NOAA's Pacific Islands Fisheries Science Center and CReefs, in accordance with permit numbers NWHIMNM-2006-015, 2006-01, 2006-017, and DLNR.NWHI06R021 and associated amendments. Florida Institute of Oceanography, University of Florida, and Sultan Qaboos University.