Supplementary Figure S1 Functional Characterisation of Snmp:GFP
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Overexpression of Salicylic Acid Carboxyl Methyltransferase (Cssamt1) Enhances Tolerance to Huanglongbing Disease in Wanjincheng Orange (Citrus Sinensis (L.) Osbeck)
International Journal of Molecular Sciences Article Overexpression of Salicylic Acid Carboxyl Methyltransferase (CsSAMT1) Enhances Tolerance to Huanglongbing Disease in Wanjincheng Orange (Citrus sinensis (L.) Osbeck) Xiuping Zou * , Ke Zhao, Yunuo Liu, Meixia Du, Lin Zheng, Shuai Wang, Lanzhen Xu, Aihong Peng, Yongrui He, Qin Long and Shanchun Chen * Citrus Research Institute, Southwest University/Chinese Academy of Agricultural Sciences, Chongqing 400716, China; [email protected] (K.Z.); [email protected] (Y.L.); [email protected] (M.D.); [email protected] (L.Z.); [email protected] (S.W.); [email protected] (L.X.); [email protected] (A.P.); [email protected] (Y.H.); [email protected] (Q.L.) * Correspondence: [email protected] (X.Z.); [email protected] (S.C.) Abstract: Citrus Huanglongbing (HLB) disease or citrus greening is caused by Candidatus Liberibacter asiaticus (Las) and is the most devastating disease in the global citrus industry. Salicylic acid (SA) plays a central role in regulating plant defenses against pathogenic attack. SA methyltransferase (SAMT) modulates SA homeostasis by converting SA to methyl salicylate (MeSA). Here, we report on the functions of the citrus SAMT (CsSAMT1) gene from HLB-susceptible Wanjincheng orange Citation: Zou, X.; Zhao, K.; Liu, Y.; (Citrus sinensis (L.) Osbeck) in plant defenses against Las infection. The CsSAMT1 cDNA was Du, M.; Zheng, L.; Wang, S.; Xu, L.; expressed in yeast. Using in vitro enzyme assays, yeast expressing CsSAMT1 was confirmed to Peng, A.; He, Y.; Long, Q.; et al. specifically catalyze the formation of MeSA using SA as a substrate. Transgenic Wanjincheng orange Overexpression of Salicylic Acid plants overexpressing CsSAMT1 had significantly increased levels of SA and MeSA compared to Carboxyl Methyltransferase wild-type controls. -
Aberrant Expression of Tetraspanin Molecules in B-Cell Chronic Lymphoproliferative Disorders and Its Correlation with Normal B-Cell Maturation
Leukemia (2005) 19, 1376–1383 & 2005 Nature Publishing Group All rights reserved 0887-6924/05 $30.00 www.nature.com/leu Aberrant expression of tetraspanin molecules in B-cell chronic lymphoproliferative disorders and its correlation with normal B-cell maturation S Barrena1,2, J Almeida1,2, M Yunta1,ALo´pez1,2, N Ferna´ndez-Mosteirı´n3, M Giralt3, M Romero4, L Perdiguer5, M Delgado1, A Orfao1,2 and PA Lazo1 1Instituto de Biologı´a Molecular y Celular del Ca´ncer, Centro de Investigacio´n del Ca´ncer, Consejo Superior de Investigaciones Cientı´ficas-Universidad de Salamanca, Salamanca, Spain; 2Servicio de Citometrı´a, Universidad de Salamanca and Hospital Universitario de Salamanca, Salamanca, Spain; 3Servicio de Hematologı´a, Hospital Universitario Miguel Servet, Zaragoza, Spain; 4Hematologı´a-hemoterapia, Hospital Universitario Rı´o Hortega, Valladolid, Spain; and 5Servicio de Hematologı´a, Hospital de Alcan˜iz, Teruel, Spain Tetraspanin proteins form signaling complexes between them On the cell surface, tetraspanin antigens are present either as and with other membrane proteins and modulate cell adhesion free molecules or through interaction with other proteins.25,26 and migration properties. The surface expression of several tetraspanin antigens (CD9, CD37, CD53, CD63, and CD81), and These interacting proteins include other tetraspanins, integri- F 22,27–30F their interacting proteins (CD19, CD21, and HLA-DR) were ns particularly those with the b1 subunit HLA class II 31–33 34,35 analyzed during normal B-cell maturation and compared to a moleculesFeg HLA DR -, CD19, the T-cell recep- group of 67 B-cell neoplasias. Three patterns of tetraspanin tor36,37 and several other members of the immunoglobulin expression were identified in normal B cells. -
Design, Development, and Characterization of Novel Antimicrobial Peptides for Pharmaceutical Applications Yazan H
University of Arkansas, Fayetteville ScholarWorks@UARK Theses and Dissertations 8-2013 Design, Development, and Characterization of Novel Antimicrobial Peptides for Pharmaceutical Applications Yazan H. Akkam University of Arkansas, Fayetteville Follow this and additional works at: http://scholarworks.uark.edu/etd Part of the Biochemistry Commons, Medicinal and Pharmaceutical Chemistry Commons, and the Molecular Biology Commons Recommended Citation Akkam, Yazan H., "Design, Development, and Characterization of Novel Antimicrobial Peptides for Pharmaceutical Applications" (2013). Theses and Dissertations. 908. http://scholarworks.uark.edu/etd/908 This Dissertation is brought to you for free and open access by ScholarWorks@UARK. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of ScholarWorks@UARK. For more information, please contact [email protected], [email protected]. Design, Development, and Characterization of Novel Antimicrobial Peptides for Pharmaceutical Applications Design, Development, and Characterization of Novel Antimicrobial Peptides for Pharmaceutical Applications A Dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Cell and Molecular Biology by Yazan H. Akkam Jordan University of Science and Technology Bachelor of Science in Pharmacy, 2001 Al-Balqa Applied University Master of Science in Biochemistry and Chemistry of Pharmaceuticals, 2005 August 2013 University of Arkansas This dissertation is approved for recommendation to the Graduate Council. Dr. David S. McNabb Dissertation Director Professor Roger E. Koeppe II Professor Gisela F. Erf Committee Member Committee Member Professor Ralph L. Henry Dr. Suresh K. Thallapuranam Committee Member Committee Member ABSTRACT Candida species are the fourth leading cause of nosocomial infection. The increased incidence of drug-resistant Candida species has emphasized the need for new antifungal drugs. -
The Tetraspanin TSPAN33 Controls TLR-Triggered Macrophage Activation Through Modulation of NOTCH Signaling
The Tetraspanin TSPAN33 Controls TLR-Triggered Macrophage Activation through Modulation of NOTCH Signaling This information is current as Almudena Ruiz-García, Susana López-López, José Javier of September 25, 2021. García-Ramírez, Victoriano Baladrón, María José Ruiz-Hidalgo, Laura López-Sanz, Ángela Ballesteros, Jorge Laborda, Eva María Monsalve and María José M. Díaz-Guerra J Immunol published online 29 August 2016 Downloaded from http://www.jimmunol.org/content/early/2016/08/27/jimmun ol.1600421 Supplementary http://www.jimmunol.org/content/suppl/2016/08/27/jimmunol.160042 http://www.jimmunol.org/ Material 1.DCSupplemental Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists by guest on September 25, 2021 • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2016 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Published August 29, 2016, doi:10.4049/jimmunol.1600421 The Journal of Immunology The Tetraspanin TSPAN33 Controls TLR-Triggered Macrophage Activation through Modulation of NOTCH Signaling Almudena Ruiz-Garcı´a,1 Susana Lo´pez-Lo´pez,1 Jose´ Javier Garcı´a-Ramı´rez, Victoriano Baladro´n, Marı´a Jose´ Ruiz-Hidalgo, Laura Lo´pez-Sanz, A´ ngela Ballesteros, Jorge Laborda, Eva Marı´a Monsalve, and Marı´a Jose´ M. -
IRON-REGULATORY FUNCTION of HEPCIDIN in the CHANNEL CATFISH and WESTERN CLAWED FROG Except Where Reference Is Made to the Work
IRON-REGULATORY FUNCTION OF HEPCIDIN IN THE CHANNEL CATFISH AND WESTERN CLAWED FROG Except where reference is made to the work of others, the work described in this dissertation is my own or was done in collaboration with my advisory committee. This dissertation does not include proprietary or classified information. __________________________ Xueyou Hu Certificate of Approval: __________________________ __________________________ Jishu Shi, Co-Chair Edward E. Morrison, Co-Chair Assistant Professor Professor Anatomy, Physiology and Anatomy, Physiology and Pharmacology Pharmacology __________________________ __________________________ Robert J. Kemppainen Christine C. Dykstra Professor Associate Professor Anatomy, Physiology and Pathobiology Pharmacology __________________________ __________________________ Yingzi Cong Joe F. Pittman Assistant Professor Interim Dean Medicine Graduate School IRON-REGULATORY FUNCTION OF HEPCIDIN IN THE CHANNEL CATFISH AND WESTERN CLAWED FROG Xueyou Hu A Dissertation Submitted to the Graduate Faculty of Auburn University in Partial Fulfillment of the Requirement for the Degree of Doctor of Philosophy Auburn, Alabama May 10, 2008 IRON-REGULATORY FUNCTION OF HEPCIDIN IN THE CHANNEL CATFISH AND WESTERN CLAWED FROG XUEYOU HU Permission is granted to Auburn University to make copies of this dissertation at its discretion, upon request of individuals or institutions at their expense. The author reserves all publication rights. ______________________________ Signature of Author ______________________________ Date -
Regeneron Science Talent Search 2021 Scholars 2021 Scholars
REGENERON SCIENCE TALENT SEARCH 2021 SCHOLARS 2021 SCHOLARS The Regeneron Science Talent Search (Regeneron STS), a program of Society for Science, is the nation’s most prestigious science and math competition for high school seniors. Alumni of STS have made extraordinary contributions to science and hold more than 100 of the world’s most distinguished science and math honors, including the Nobel Prize and the National Medal of Science. Each year, 300 Regeneron STS scholars and their schools are recognized. From that select pool of scholars, 40 student finalists are invited to participate in final judging, display their work to the public, meet with notable scientists and compete for awards, including the top award of $250,000. Regeneron Science Talent Search, A Program of Society for Science ARIZONA Scottsdale BASIS Scottsdale Mehta, Viraj, 18 GLIA-Deep: Glioblastoma Image Analysis Using Deep Learning Convolutional Neural Networks to Accurately Classify Gene Methylation and Predict Drug Effectiveness CALIFORNIA Arcadia Arcadia High School Liu, Stanley Cheung, 17 A Microfluidic Device for Blood Plasma Separation and Fluorescence Detection of Biomarkers Using Acoustic Microstreaming Atherton Menlo School Tung, Katherine, 17 The Sperner Property for 132-Avoiding Intervals in the Weak Order Cupertino Cupertino High School Agrawal, Vardhan, 18 BP-Lytic: A Novel Apparatus to Continuously, Cufflessly, and Affordably Monitor Blood Pressure Trends Homestead High School Sanyal, Anushka, 17 Intronic RNA Lariats Protect Against Neurodegenerative Disease -
Optimizing Exogenous Surfactant As a Pulmonary Delivery Vehicle for Chicken Cathelicidin-2
Lawrence Berkeley National Laboratory Recent Work Title Optimizing Exogenous Surfactant as a Pulmonary Delivery Vehicle for Chicken Cathelicidin-2. Permalink https://escholarship.org/uc/item/9bk2h47d Journal Scientific reports, 10(1) ISSN 2045-2322 Authors Baer, Brandon Veldhuizen, Edwin JA Molchanova, Natalia et al. Publication Date 2020-06-10 DOI 10.1038/s41598-020-66448-1 Peer reviewed eScholarship.org Powered by the California Digital Library University of California www.nature.com/scientificreports OPEN Optimizing Exogenous Surfactant as a Pulmonary Delivery Vehicle for Chicken Cathelicidin-2 Brandon Baer1 ✉ , Edwin J. A. Veldhuizen2, Natalia Molchanova3,4, Shehrazade Jekhmane5, Markus Weingarth5, Håvard Jenssen3, Jennifer S. Lin6, Annelise E. Barron6, Cory Yamashita1,7 & Ruud Veldhuizen1,7 The rising incidence of antibiotic-resistant lung infections has instigated a much-needed search for new therapeutic strategies. One proposed strategy is the use of exogenous surfactants to deliver antimicrobial peptides (AMPs), like CATH-2, to infected regions of the lung. CATH-2 can kill bacteria through a diverse range of antibacterial pathways and exogenous surfactant can improve pulmonary drug distribution. Unfortunately, mixing AMPs with commercially available exogenous surfactants has been shown to negatively impact their antimicrobial function. It was hypothesized that the phosphatidylglycerol component of surfactant was inhibiting AMP function and that an exogenous surfactant, with a reduced phosphatidylglycerol composition would increase peptide mediated killing at a distal site. To better understand how surfactant lipids interacted with CATH-2 and afected its function, isothermal titration calorimetry and solid-state nuclear magnetic resonance spectroscopy as well as bacterial killing curves against Pseudomonas aeruginosa were utilized. Additionally, the wet bridge transfer system was used to evaluate surfactant spreading and peptide transport. -
Magainin 2 in Action: Distinct Modes of Membrane Permeabilization in Living Bacterial and Mammalian Cells
Biophysical Journal Volume 95 December 2008 5757–5765 5757 Magainin 2 in Action: Distinct Modes of Membrane Permeabilization in Living Bacterial and Mammalian Cells Yuichi Imura, Naoki Choda, and Katsumi Matsuzaki Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo-Ku, Kyoto 606-8501, Japan ABSTRACT Interactions of cationic antimicrobial peptides with living bacterial and mammalian cells are little understood, although model membranes have been used extensively to elucidate how peptides permeabilize membranes. In this study, the interaction of F5W-magainin 2 (GIGKWLHSAKKFGKAFVGEIMNS), an equipotent analogue of magainin 2 isolated from the African clawed frog Xenopus laevis, with unfixed Bacillus megaterium and Chinese hamster ovary (CHO)-K1 cells was investigated, using confocal laser scanning microscopy. A small amount of tetramethylrhodamine-labeled F5W-magainin 2 was incorporated into the unlabeled peptide for imaging. The influx of fluorescent markers of various sizes into the cytosol revealed that magainin 2 permeabilized bacterial and mammalian membranes in significantly different ways. The peptide formed pores with a diameter of ;2.8 nm (, 6.6 nm) in B. megaterium, and translocated into the cytosol. In contrast, the peptide significantly perturbed the membrane of CHO-K1 cells, permitting the entry of a large molecule (diameter, .23 nm) into the cytosol, accompanied by membrane budding and lipid flip-flop, mainly accumulating in mitochondria and nuclei. Adenosine triphosphate and negatively charged glycosaminoglycans were little involved in the magainin-induced permeabilization of membranes in CHO-K1 cells. Furthermore, the susceptibility of CHO-K1 cells to magainin was found to be similar to that of erythrocytes. Thus, the distinct membrane-permeabilizing processes of magainin 2 in bacterial and mammalian cells were, to the best of our knowledge, visualized and characterized in detail for the first time. -
Biogenesis of Extracellular Vesicles (EV): Exosomes, Microvesicles, Retrovirus-Like Vesicles, and Apoptotic Bodies
J Neurooncol (2013) 113:1–11 DOI 10.1007/s11060-013-1084-8 TOPIC REVIEW Biogenesis of extracellular vesicles (EV): exosomes, microvesicles, retrovirus-like vesicles, and apoptotic bodies Johnny C. Akers • David Gonda • Ryan Kim • Bob S. Carter • Clark C. Chen Received: 27 July 2012 / Accepted: 13 February 2013 / Published online: 2 March 2013 Ó Springer Science+Business Media New York 2013 Abstract Recent studies suggest both normal and can- Abbreviations cerous cells secrete vesicles into the extracellular space. Exosomes 30–100 nm secreted vesicles that These extracellular vesicles (EVs) contain materials that originate from the endosomal mirror the genetic and proteomic content of the secreting network cell. The identification of cancer-specific material in EVs Microvesicles 50–2,000 nm vesicles that arise isolated from the biofluids (e.g., serum, cerebrospinal fluid, through direct outward budding urine) of cancer patients suggests EVs as an attractive and fission of the plasma mem- platform for biomarker development. It is important to brane recognize that the EVs derived from clinical samples are Retrovirus-like particles 90–100 nm non-infectious vesicles likely highly heterogeneous in make-up and arose from that resemble retroviral vesicles diverse sets of biologic processes. This article aims to and contain a subset of retroviral review the biologic processes that give rise to various types proteins of EVs, including exosomes, microvesicles, retrovirus like Apoptotic bodies 50–5,000 nm vesicles produced particles, and apoptotic bodies. Clinical pertinence of these from cell undergoing cell death EVs to neuro-oncology will also be discussed. by apoptosis EV Extracellular vesicle Keywords Biomarkers Á Intracellular trafficking Á RLP Retrovirus like particle Membrane budding Á Tetraspanin Á Multi-vesicular bodies ILV Intraluminal vesicle (MVB) Á Tumor microenvironment Á Cancer MVB Multivesicular body TEM Tetraspanin enriched microdo- main ESCRT Endosomal sorting complex required for transport Bob S. -
Supp Table 6.Pdf
Supplementary Table 6. Processes associated to the 2037 SCL candidate target genes ID Symbol Entrez Gene Name Process NM_178114 AMIGO2 adhesion molecule with Ig-like domain 2 adhesion NM_033474 ARVCF armadillo repeat gene deletes in velocardiofacial syndrome adhesion NM_027060 BTBD9 BTB (POZ) domain containing 9 adhesion NM_001039149 CD226 CD226 molecule adhesion NM_010581 CD47 CD47 molecule adhesion NM_023370 CDH23 cadherin-like 23 adhesion NM_207298 CERCAM cerebral endothelial cell adhesion molecule adhesion NM_021719 CLDN15 claudin 15 adhesion NM_009902 CLDN3 claudin 3 adhesion NM_008779 CNTN3 contactin 3 (plasmacytoma associated) adhesion NM_015734 COL5A1 collagen, type V, alpha 1 adhesion NM_007803 CTTN cortactin adhesion NM_009142 CX3CL1 chemokine (C-X3-C motif) ligand 1 adhesion NM_031174 DSCAM Down syndrome cell adhesion molecule adhesion NM_145158 EMILIN2 elastin microfibril interfacer 2 adhesion NM_001081286 FAT1 FAT tumor suppressor homolog 1 (Drosophila) adhesion NM_001080814 FAT3 FAT tumor suppressor homolog 3 (Drosophila) adhesion NM_153795 FERMT3 fermitin family homolog 3 (Drosophila) adhesion NM_010494 ICAM2 intercellular adhesion molecule 2 adhesion NM_023892 ICAM4 (includes EG:3386) intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)adhesion NM_001001979 MEGF10 multiple EGF-like-domains 10 adhesion NM_172522 MEGF11 multiple EGF-like-domains 11 adhesion NM_010739 MUC13 mucin 13, cell surface associated adhesion NM_013610 NINJ1 ninjurin 1 adhesion NM_016718 NINJ2 ninjurin 2 adhesion NM_172932 NLGN3 neuroligin -
(12) United States Patent (10) Patent No.: US 7,829,086 B2 Hilbert Et Al
US007829086B2 (12) United States Patent (10) Patent No.: US 7,829,086 B2 Hilbert et al. (45) Date of Patent: Nov. 9, 2010 (54) HUMANIZED ANTI-CD22 ANTIBODIES AND 2004/0202658 A1 10/2004 Benyunes THEIR USE IN TREATMENT OF 2004/021915.6 A1 11/2004 Goldenberg et al. ONCOLOGY, TRANSPLANTATION AND 2005, 0118182 A1 6/2005 Pastan et al. AUTOIMMUNE DISEASE 2007,0264260 A1 11/2007 Tuscano et al. (75) Inventors: David M. Hilbert, Bethesda, MD (US); 2008.0118505 A1 5/2008 Tedder Tarran Jones, Radlett (GB); David G. Williams, Epsom (GB) (73) Assignees: Medimmune, LLC, Gaithersburg, MD FOREIGN PATENT DOCUMENTS (US); Aeres Biomedical, Ltd., London EP O 660 721 B1 10, 2008 (GB) WO WOO3,O93320 A2 11/2003 (*) Notice: Subject to any disclaimer, the term of this patent is extended or adjusted under 35 U.S.C. 154(b) by 132 days. OTHER PUBLICATIONS (21) Appl. No.: 11/715,308 MacCallum et al. (J. Mol. Biol. (1996) 262:732-745).* De Pascalis et al. (The Journal of Immunology (2002) 169, 3076 (22) Filed: Mar. 6, 2007 3084).* (65) Prior Publication Data Casset et al. (2003) BBRC 307, 198-205).* Vajdos et al. (2002) J. Mol. Biol. 320, 415-428).* US 2007/O258981 A1 Nov. 8, 2007 Holmetal ((2007) Mol. Immunol. 44: 1075-1084).* Related U.S. Application Data Chen et al. (J. Mol. Bio. (1999) 293, 865-881).* Wu et al. (J. Mol. Biol. (1999) 294, 151-162).* (60) Provisional application No. 60/779,806, filed on Mar. Brummell et al. (Biochemistry 32:1180-1187 (1993)).* 6, 2006. -
Human Induced Pluripotent Stem Cell–Derived Podocytes Mature Into Vascularized Glomeruli Upon Experimental Transplantation
BASIC RESEARCH www.jasn.org Human Induced Pluripotent Stem Cell–Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation † Sazia Sharmin,* Atsuhiro Taguchi,* Yusuke Kaku,* Yasuhiro Yoshimura,* Tomoko Ohmori,* ‡ † ‡ Tetsushi Sakuma, Masashi Mukoyama, Takashi Yamamoto, Hidetake Kurihara,§ and | Ryuichi Nishinakamura* *Department of Kidney Development, Institute of Molecular Embryology and Genetics, and †Department of Nephrology, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan; ‡Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Hiroshima, Japan; §Division of Anatomy, Juntendo University School of Medicine, Tokyo, Japan; and |Japan Science and Technology Agency, CREST, Kumamoto, Japan ABSTRACT Glomerular podocytes express proteins, such as nephrin, that constitute the slit diaphragm, thereby contributing to the filtration process in the kidney. Glomerular development has been analyzed mainly in mice, whereas analysis of human kidney development has been minimal because of limited access to embryonic kidneys. We previously reported the induction of three-dimensional primordial glomeruli from human induced pluripotent stem (iPS) cells. Here, using transcription activator–like effector nuclease-mediated homologous recombination, we generated human iPS cell lines that express green fluorescent protein (GFP) in the NPHS1 locus, which encodes nephrin, and we show that GFP expression facilitated accurate visualization of nephrin-positive podocyte formation in