APP regulates male sexual behavior 1

Supplemental Figure 1. Principle components analysis of the maters (red diamonds labeled M) versus non-maters (black circles labeled C) where each point represents a intensity data from one microarray hybridization. The two principle components axes, PC1 and PC2, represent directions associated with the highest and the second highest degree of variability in the data of all ~40,000 on the array.

APP regulates male sexual behavior 2

Supplemental Table 1. 532 differentially expressed genes after applying a 5% False Discovery Rate cutoff between maters and non-maters (n=5/group). Listed are the symbols, description, p-value and fold difference.

Supplemental Table 1 p- Fold Gene Symbol Description value ∆ Pabpc1 poly A binding , cytoplasmic 1 0.036 2.62 D630023F18Rik RIKEN cDNA D630023F18 gene 0.036 1.78 Ccdc82 coiled-coil domain containing 82 0.015 1.66 Cdt1 licensing and DNA replication factor 1 0.021 1.65 Mmrn2 multimerin 2 0.035 1.62 Efcbp1 EF hand calcium binding protein 1 0.04 1.61 Rrm1 ribonucleotide reductase M1 0.042 1.59 Cox7c cytochrome c oxidase, subunit VIIc 0.04 1.57 Bdnf brain derived neurotrophic factor 0.034 1.52 Sox11 SRY-box containing gene 11 0.048 1.52 Srr serine racemase 0.012 1.49 Zfp291 protein 291 0.038 1.49 Setmar SET domain and mariner transposase fusion gene 0.043 1.49 AI462438 expressed sequence AI462438 0.023 1.48 Zipro1 zinc finger proliferation 1 0.044 1.47 Suv39h1 suppressor of variegation 3-9 homolog 1 (Drosophila) 0.022 1.45 Zfp62 zinc finger protein 62 0.048 1.45 1110057K04Rik RIKEN cDNA 1110057K04 gene 0.037 1.44 Mak10 MAK10 homolog, amino-acid N-acetyltransferase subunit, (S. cerevisiae) 0.021 1.43 Sap30bp SAP30 binding protein 0.036 1.43 Snip1 Smad nuclear interacting protein 1 0.04 1.42 Rbm18 RNA binding motif protein 18 0.037 1.41 Ehd1 EH-domain containing 1 0.047 1.4 1700052N19Rik RIKEN cDNA 1700052N19 gene 0.021 1.39 2700062C07Rik RIKEN cDNA 2700062C07 gene 0.023 1.39 Serf1 small EDRK-rich factor 1 0.047 1.39 1700034M03Rik RIKEN cDNA 1700034M03 gene 0.031 1.39 Cntn1 contactin 1 0.036 1.39 Epha4 Eph A4 0.043 1.39 Rab39b RAB39B, member RAS oncogene family 0.043 1.38 LOC381255 0.046 1.38 Trim2 tripartite motif protein 2 0.047 1.37 BC066028 cDNA sequence BC066028 0.036 1.36 1110020G09Rik RIKEN cDNA 1110020G09 gene 0.036 1.36 Cryzl1 crystallin, zeta (quinone reductase)-like 1 0.046 1.36 Gnpda2 glucosamine-6-phosphate deaminase 2 0.049 1.36 Mtap methylthioadenosine phosphorylase 0.038 1.35 Josd3 Josephin domain containing 3 0.039 1.35 Arf2 ADP-ribosylation factor 2 0.043 1.35 Ankrd45 domain 45 0.049 1.35 Ints12 integrator complex subunit 12 0.041 1.34 Cml1 camello-like 1 0.041 1.34 Tatdn1 TatD DNase domain containing 1 0.044 1.34 APP regulates male sexual behavior 3

Pten phosphatase and tensin homolog 0.021 1.33 Kbtbd3 kelch repeat and BTB (POZ) domain containing 3 0.032 1.33 Lgtn ligatin 0.041 1.33 Ttc32 tetratricopeptide repeat domain 32 0.042 1.33 Epha4 Eph receptor A4 0.045 1.33 F2r coagulation factor II () receptor 0.045 1.33 Mnat1 menage a trois 1 0.045 1.33 Btbd5 BTB (POZ) domain containing 5 0.021 1.32 Sgcb sarcoglycan, beta (dystrophin-associated glycoprotein) 0.038 1.32 C730024G19Rik RIKEN cDNA C730024G19 gene 0.041 1.32 Hist3h2a histone 3, H2a 0.044 1.32 Lnx1 ligand of numb-protein X 1 0.048 1.32 2900057D21Rik RIKEN cDNA 2900057D21 gene 0.01 1.31 Arpp19 cAMP-regulated phosphoprotein 19 0.015 1.31 Lgi1 leucine-rich repeat LGI family, member 1 0.022 1.31 4932415D10Rik RIKEN cDNA 4932415D10 gene 0.029 1.31 H2afj H2A histone family, member J 0.04 1.31 Sod1 superoxide dismutase 1, soluble 0.012 1.3 Stau2 staufen (RNA binding protein) homolog 2 (Drosophila) 0.019 1.29 AI256775 expressed sequence AI256775 0.036 1.29 Bxdc5 brix domain containing 5 0.045 1.29 Ufm1 ubiquitin-fold modifier 1 0.05 1.29 Ppp2r3a protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha 0.05 1.29 Lztfl1 -like 1 0.022 1.28 LOC208595 0.025 1.28 Idh3a isocitrate dehydrogenase 3 (NAD+) alpha 0.029 1.28 Folh1 folate hydrolase 0.032 1.28 Arg2 arginase type II 0.036 1.28 Fads1 fatty acid desaturase 1 0.038 1.28 Scn1a sodium channel, voltage-gated, type I, alpha 0.038 1.28 Exosc1 exosome component 1 0.04 1.28 Trappc5 trafficking protein particle complex 5 0.04 1.28 Trfr2 transferrin receptor 2 0.049 1.28 Nme5 expressed in non-metastatic cells 5 0.05 1.28 1110034B05Rik RIKEN cDNA 1110034B05 gene 0.05 1.28 Pgm3 phosphoglucomutase 3 0.013 1.27 Cryzl1 crystallin, zeta (quinone reductase)-like 1 0.015 1.27 Tmem167 transmembrane protein 167 0.015 1.27 Wdr61 WD repeat domain 61 0.021 1.27 Dpm1 dolichol-phosphate (beta-D) mannosyltransferase 1 0.026 1.27 Ppil3 peptidylprolyl isomerase (cyclophilin)-like 3 0.036 1.27 Scfd1 sec1 family domain containing 1 0.019 1.27 4933433P14Rik RIKEN cDNA 4933433P14 gene 0.029 1.27 Orc3l origin recognition complex, subunit 3-like (S. cerevisiae) 0.029 1.27 Anapc10 anaphase promoting complex subunit 10 0.036 1.27 Gtf2b general transcription factor IIB 0.042 1.27 Tmem167 transmembrane protein 167 0.014 1.26 1810030O07Rik RIKEN cDNA 1810030O07 gene 0.024 1.26 Rage renal tumor antigen 0.025 1.26 2310045N01Rik RIKEN cDNA 2310045N01 gene 0.028 1.26 APP regulates male sexual behavior 4

H2afz H2A histone family, member Z 0.04 1.26 Arfrp1 ADP-ribosylation factor related protein 1 0.044 1.26 Gtdc1 glycosyltransferase-like domain containing 1 0.046 1.26 Zfp706 zinc finger protein 706 0.047 1.26 Ergic2 ERGIC and golgi 2 0.01 1.25 Mrpl21 mitochondrial L21 0.021 1.25 Exosc8 exosome component 8 0.032 1.25 Ube1c ubiquitin-activating E1C 0.034 1.25 Rnf121 ring finger protein 121 0.036 1.25 Mpp5 membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) 0.038 1.25 Stk38l serine/threonine kinase 38 like 0.042 1.25 Nup88 88 0.042 1.25 Zfp273 zinc finger protein 273 0.043 1.25 Tnrc15 trinucleotide repeat containing 15 0.047 1.25 Polr3k polymerase (RNA) III (DNA directed) polypeptide K 0.048 1.25 1110059P08Rik RIKEN cDNA 1110059P08 gene 0.05 1.25 Zfp46 zinc finger protein 46 0.019 1.24 Rpl7l1 ribosomal protein L7-like 1 0.021 1.24 Ociad2 OCIA domain containing 2 0.021 1.24 Snord22 small nucleolar RNA, C/D box 22 0.027 1.24 Trnt1 tRNA nucleotidyl transferase, CCA-adding, 1 0.035 1.24 Blzf1 basic leucine zipper nuclear factor 1 0.04 1.24 Hars histidyl-tRNA synthetase 0.04 1.24 Cdadc1 cytidine and dCMP deaminase domain containing 1 0.04 1.24 Uros uroporphyrinogen III synthase 0.045 1.24 Mrpl3 mitochondrial ribosomal protein L3 0.045 1.24 0610038F07Rik RIKEN cDNA 0610038F07 gene 0.013 1.23 Anapc10 anaphase promoting complex subunit 10 0.021 1.23 Mrpl48 mitochondrial ribosomal protein L48 0.022 1.23 A230046K03Rik RIKEN cDNA A230046K03 gene 0.023 1.23 Mrpl49 mitochondrial ribosomal protein L49 0.025 1.23 Eif3s6 eukaryotic 3, subunit 6 0.029 1.23 0610038F07Rik RIKEN cDNA 0610038F07 gene 0.031 1.23 Mak10 MAK10 homolog, amino-acid N-acetyltransferase subunit, (S. cerevisiae) 0.036 1.23 Rg9mtd3 RNA (guanine-9-) methyltransferase domain containing 3 0.038 1.23 Igsf1 immunoglobulin superfamily, member 1 0.04 1.23 Eif2s1 initiation factor 2, subunit 1 alpha 0.04 1.23 Mtg1 mitochondrial GTPase 1 homolog (S. cerevisiae) 0.04 1.23 Sugt1 SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) 0.042 1.23 Pigf phosphatidylinositol glycan anchor biosynthesis, class F 0.045 1.23 Rabl4 RAB, member of RAS oncogene family-like 4 0.047 1.23 Snupn snurportin 1 0.048 1.23 BC011248 cDNA sequence BC011248 0.049 1.23 C130086A10 0.01 1.22 Acn9 ACN9 homolog (S. cerevisiae) 0.028 1.22 Creb3 cAMP responsive element binding protein 3 0.03 1.22 1700026B20Rik RIKEN cDNA 1700026B20 gene 0.036 1.22 Cdc5l cell division cycle 5-like (S. pombe) 0.038 1.22 Man1a2 mannosidase, alpha, class 1A, member 2 0.041 1.22 Kpna2 karyopherin (importin) alpha 2 0.042 1.22 APP regulates male sexual behavior 5

Tmem128 transmembrane protein 128 0.048 1.22 Coq3 coenzyme Q3 homolog, methyltransferase (yeast) 0.01 1.21 Pigx phosphatidylinositol glycan anchor biosynthesis, class X 0.022 1.21 1500011J06Rik RIKEN cDNA 1500011J06 gene 0.026 1.21 Mrps33 mitochondrial ribosomal protein S33 0.037 1.21 Jagn1 jagunal homolog 1 (Drosophila) 0.037 1.21 Commd6 COMM domain containing 6 0.038 1.21 Ssbp1 single-stranded DNA binding protein 1 0.044 1.21 Gpr137b G protein-coupled receptor 137B 0.046 1.21 2610029G23Rik RIKEN cDNA 2610029G23 gene 0.028 1.21 Hirip5 histone regulation defective interacting protein 5 0.037 1.21 Mrpl35 mitochondrial ribosomal protein L35 0.04 1.21 Bdh1 3-hydroxybutyrate dehydrogenase, type 1 0.042 1.21 Ddx52 DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 0.043 1.21 Tbc1d15 TBC1 domain family, member 15 0.043 1.21 BC023829 cDNA sequence BC023829 0.043 1.21 Ccdc32 coiled-coil domain containing 32 0.044 1.21 Asns asparagine synthetase 0.045 1.21 Immp1l IMP1 inner mitochondrial membrane peptidase-like (S. cerevisiae) 0.046 1.21 Trmt5 TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) 0.046 1.21 Anapc5 anaphase-promoting complex subunit 5 0.048 1.21 0610007P06Rik RIKEN cDNA 0610007P06 gene 0.021 1.2 Ccnh cyclin H 0.022 1.2 Ga17 dendritic cell protein GA17 0.023 1.2 D10Wsu52e DNA segment, Chr 10, Wayne State University 52, expressed 0.025 1.2 Zfp207 zinc finger protein 207 0.025 1.2 Rnf34 ring finger protein 34 0.026 1.2 Lsm3 LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae) 0.029 1.2 1810022K09Rik RIKEN cDNA 1810022K09 gene 0.038 1.2 Ppp1r7 protein phosphatase 1, regulatory (inhibitor) subunit 7 0.04 1.2 Ipo11 importin 11 0.045 1.2 B230118H07Rik RIKEN cDNA B230118H07 gene 0.047 1.2 Rpa2 replication protein A2 0.048 1.2 Cdkal1 CDK5 regulatory subunit associated protein 1-like 1 0.048 1.2 Lta4h leukotriene A4 hydrolase 0.021 1.19 Tssc1 tumor suppressing subtransferable candidate 1 0.037 1.19 Mcts1 malignant T cell amplified sequence 1 0.042 1.19 Pop7 processing of precursor 7, ribonuclease P family, (S. cerevisiae) 0.042 1.19 Msra sulfoxide reductase A 0.044 1.19 2700085E05Rik RIKEN cDNA 2700085E05 gene 0.047 1.19 Ttc1 tetratricopeptide repeat domain 1 0.048 1.19 A430005L14Rik RIKEN cDNA A430005L14 gene 0.05 1.19 0610008C08Rik RIKEN cDNA 0610008C08 gene 0.012 1.18 Cryzl1 crystallin, zeta (quinone reductase)-like 1 0.022 1.18 1190005P17Rik RIKEN cDNA 1190005P17 gene 0.024 1.18 Smu1 smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans) 0.036 1.18 Snx7 7 0.038 1.18 2610204K14Rik RIKEN cDNA 2610204K14 gene 0.038 1.18 Atp6v1e1 VATPase, H+ transporting, lysosomal V1 subunit E1 0.041 1.18 Wdr74 WD repeat domain 74 0.042 1.18 APP regulates male sexual behavior 6

1810003N24Rik RIKEN cDNA 1810003N24 gene 0.044 1.18 Gstt1 glutathione S-transferase, theta 1 0.046 1.18 Stx18 syntaxin 18 0.049 1.18 Rbmxrt RNA binding motif protein, X retrogene 0.01 1.17 B230217C12Rik RIKEN cDNA B230217C12 gene 0.022 1.17 Ddx47 DEAD (Asp-Glu-Ala-Asp) box polypeptide 47 0.036 1.17 Prei3 preimplantation protein 3 0.036 1.17 Psmd7 proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 0.038 1.17 Cdc42 cell division cycle 42 homolog (S. cerevisiae) 0.038 1.17 Azin1 antizyme inhibitor 1 0.04 1.17 1500011K16Rik RIKEN cDNA 1500011K16 gene 0.042 1.17 Brcc3 BRCA1/BRCA2-containing complex, subunit 3 0.013 1.16 Med31 mediator of RNA polymerase II transcription, subunit 31 homolog (yeast) 0.025 1.16 Polr2e polymerase (RNA) II (DNA directed) polypeptide E 0.025 1.16 Ftsj1 Ftsj homolog 1 (E. coli) 0.036 1.16 Prmt7 protein arginine N-methyltransferase 7 0.038 1.16 AI788669 expressed sequence AI788669 0.04 1.16 Rnuxa RNA U, small nuclear RNA export adaptor 0.045 1.16 Tmem30a transmembrane protein 30A 0.045 1.16 Xrcc6bp1 XRCC6 binding protein 1 0.046 1.16 Nicn1 nicolin 1 0.046 1.16 0610007P06Rik RIKEN cDNA 0610007P06 gene 0.049 1.16 1700025K23Rik RIKEN cDNA 1700025K23 gene 0.01 1.16 Snrpd2 small nuclear ribonucleoprotein D2 0.012 1.16 Trappc2 trafficking protein particle complex 2 0.021 1.16 Mbd2 methyl-CpG binding domain protein 2 0.028 1.16 Impa1 inositol (myo)-1(or 4)-monophosphatase 1 0.029 1.16 A030007L17Rik RIKEN cDNA A030007L17 gene 0.036 1.16 Narfl nuclear prelamin A recognition factor-like 0.038 1.16 Eif3s6 eukaryotic translation initiation factor 3, subunit 6 0.041 1.16 Cox6b1 cytochrome c oxidase, subunit VIb polypeptide 1 0.042 1.16 Eif3s6 eukaryotic translation initiation factor 3, subunit 6 0.043 1.16 Rps7 ribosomal protein S7 0.05 1.16 Hirip5 histone cell cycle regulation defective interacting protein 5 0.018 1.15 Hip2 huntingtin interacting protein 2 0.021 1.15 Psmc1 protease (prosome, macropain) 26S subunit, ATPase 1 0.021 1.15 Trappc2l trafficking protein particle complex 2-like 0.021 1.15 Brms1l breast cancer metastasis-suppressor 1-like 0.022 1.15 Cops5 COP9 (constitutive photomorphogenic) homolog, subunit 5 (Arabidopsis thaliana) 0.022 1.15 Creb3 cAMP responsive element binding protein 3 0.041 1.15 Psma4 proteasome (prosome, macropain) subunit, alpha type 4 0.041 1.15 Ap4s1 adaptor-related AP-4, sigma 1 0.044 1.15 Cript cysteine-rich PDZ-binding protein 0.045 1.15 Wdr85 WD40 repeat domain 85 0.048 1.15 Psma5 proteasome (prosome, macropain) subunit, alpha type 5 0.032 1.14 0610009D07Rik RIKEN cDNA 0610009D07 gene 0.038 1.14 Rps6 0.045 1.14 Ube2f ubiquitin-conjugating enzyme (putative) 0.045 1.14 Polr3k polymerase (RNA) III (DNA directed) polypeptide K 0.046 1.14 Cyb5r1 cytochrome b5 reductase 1 0.048 1.14 APP regulates male sexual behavior 7

Cyp2j9 cytochrome P450, family 2, subfamily j, polypeptide 9 0.049 1.14 Fxc1 fractured callus expressed transcript 1 0.038 1.13 Uchl5 ubiquitin carboxyl-terminal esterase L5 0.04 1.13 Rpl18 ribosomal protein L18 0.04 1.13 1810035L17Rik RIKEN cDNA 1810035L17 gene 0.041 1.13 Idh3a isocitrate dehydrogenase 3 (NAD+) alpha 0.043 1.13 Blvra biliverdin reductase A 0.044 1.13 Dync2li1 dynein cytoplasmic 2 light intermediate chain 1 0.046 1.13 Rabepk Rab9 effector protein with kelch motifs 0.048 1.13 2700029M09Rik RIKEN cDNA 2700029M09 gene 0.049 1.13 6530401D17Rik RIKEN cDNA 6530401D17 gene 0.021 1.13 Ubl4 ubiquitin-like 4 0.024 1.13 Grpel1 GrpE-like 1, mitochondrial 0.027 1.13 0610012D17Rik RIKEN cDNA 0610012D17 gene 0.034 1.13 Nt5c2 5'-nucleotidase, cytosolic II 0.04 1.13 Sra1 steroid receptor RNA activator 1 0.045 1.13 1110005A03Rik RIKEN cDNA 1110005A03 gene 0.047 1.13 Vps29 vacuolar protein sorting 29 (S. pombe) 0.014 1.12 Rpo1-3 RNA polymerase 1-3 0.021 1.12 2610312B22Rik RIKEN cDNA 2610312B22 gene 0.036 1.12 Bag5 BCL2-associated athanogene 5 0.039 1.12 Rpl36al ribosomal protein L36a-like 0.047 1.12 Mrpl15 mitochondrial ribosomal protein L15 0.014 1.11 Maged2 melanoma antigen, family D, 2 0.04 1.11 Dnaja2 DnaJ (Hsp40) homolog, subfamily A, member 2 0.044 1.11 Ndufv1 NADH dehydrogenase (ubiquinone) flavoprotein 1 0.025 1.1 Fxc1 fractured callus expressed transcript 1 0.04 1.1 Rars arginyl-tRNA synthetase 0.042 1.1 2510003E04Rik RIKEN cDNA 2510003E04 gene 0.043 1.1 Eif3s12 eukaryotic translation initiation factor 3, subunit 12 0.047 1.1 Orc2l origin recognition complex, subunit 2-like (S. cerevisiae) 0.048 1.1 Lancl1 LanC (bacterial lantibiotic synthetase component C)-like 1 0.04 1.09 Rpl18a Ribosomal protein L18A 0.049 1.09 Magoh mago-nashi homolog, proliferation-associated (Drosophila) 0.04 1.09 Naca nascent polypeptide-associated complex alpha polypeptide 0.047 1.09 Actr10 ARP10 -related protein 10 homolog (S. cerevisiae) 0.023 1.08 Psma3 proteasome (prosome, macropain) subunit, alpha type 3 0.04 1.08 H2afz H2A histone family, member Z 0.049 1.06 Cox7a2l cytochrome c oxidase subunit VIIa polypeptide 2-like 0.038 1.06 App amyloid beta (A4) precursor protein 0.046 1.08 Csnk1a1 casein kinase 1, alpha 1 0.023 1.1 Acsl3 acyl-CoA synthetase long-chain family member 3 0.014 1.12 Zfp275 zinc finger protein 275 0.021 1.12 Reep5 receptor accessory protein 5 0.022 1.12 Ahcyl1 S-adenosylhomocysteine hydrolase-like 1 0.039 1.12 Vps4a vacuolar protein sorting 4a (yeast) 0.046 1.13 Ss18l1 synovial sarcoma translocation gene on chromosome 18-like 1 0.032 1.13 Atp1b1 ATPase, Na+/K+ transporting, beta 1 polypeptide 0.048 1.13 Syt11 synaptotagmin XI 0.036 1.14 Ptprz1 protein tyrosine phosphatase, receptor type Z, polypeptide 1 0.044 1.14 APP regulates male sexual behavior 8

Mtdh Metadherin 0.045 1.14 Mcl1 myeloid cell leukemia sequence 1 0.019 1.15 Bap1 Brca1 associated protein 1 0.019 1.15 BC060631 cDNA sequence BC060631 0.029 1.15 Sf3b2 splicing factor 3b, subunit 2 0.032 1.15 Csf1 colony stimulating factor 1 (macrophage) 0.037 1.15 Gdi2 guanosine diphosphate (GDP) dissociation inhibitor 2 0.048 1.15 Arhgef12 Rho guanine nucleotide exchange factor (GEF) 12 0.025 1.16 Hsp90ab1 heat shock protein 90kDa alpha (cytosolic), class B member 1 0.029 1.16 Cadps Ca<2+>dependent activator protein for secretion 0.038 1.16 Asxl1 additional sex combs like 1 (Drosophila) 0.025 1.17 Sh3bp5 SH3-domain binding protein 5 (BTK-associated) 0.028 1.17 Slc27a4 solute carrier family 27 (fatty acid transporter), member 4 0.037 1.17 Bace1 beta-site APP cleaving enzyme 1 0.046 1.17 Eif4g2 eukaryotic translation initiation factor 4, gamma 2 0.014 1.18 Mkrn1 makorin, ring finger protein, 1 0.022 1.18 D2Bwg1423e DNA segment, Chr 2, Brigham & Women's Genetics 1423 expressed 0.047 1.18 Fads1 fatty acid desaturase 1 0.021 1.19 Eef2 eukaryotic translation 2 0.021 1.19 Gats opposite strand transcription unit to Stag3 0.026 1.19 Camta1 calmodulin binding transcription activator 1 0.045 1.19 4632428N05Rik RIKEN cDNA 4632428N05 gene 0.045 1.19 Lasp1 LIM and SH3 protein 1 0.047 1.19 P4hb prolyl 4-hydroxylase, beta polypeptide 0.047 1.19 Tnik TRAF2 and NCK interacting kinase 0.049 1.19 2700081O15Rik RIKEN cDNA 2700081O15 gene 0.05 1.19 Pcbp2 poly(rC) binding protein 2 0.026 1.2 Gpm6b glycoprotein m6b 0.044 1.2 Acss1 acyl-CoA synthetase short-chain family member 1 0.036 1.21 Cyfip2 cytoplasmic FMR1 interacting protein 2 0.038 1.21 1810073N04Rik RIKEN cDNA 1810073N04 gene 0.038 1.21 Scamp5 secretory carrier membrane protein 5 0.038 1.21 Trib2 tribbles homolog 2 (Drosophila) 0.04 1.21 Hcfc1 host cell factor C1 0.042 1.21 Zxdc ZXD family zinc finger C 0.044 1.21 Rnf149 ring finger protein 149 0.048 1.21 Bicd2 bicaudal D homolog 2 (Drosophila) 0.049 1.21 Atp9a ATPase, class II, type 9A 0.012 1.21 Rab6ip1 Rab6 interacting protein 1 0.019 1.21 Macf1 microtubule-actin crosslinking factor 1 0.019 1.21 Trrap transformation/transcription domain-associated protein 0.021 1.21 Tex2 testis expressed gene 2 0.029 1.21 Dnajb10 DnaJ (Hsp40) homolog, subfamily B, member 10 0.043 1.21 AV009015 expressed sequence AV009015 0.043 1.21 Ptp4a2 protein tyrosine phosphatase 4a2 0.015 1.22 Chst2 carbohydrate sulfotransferase 2 0.019 1.22 Pbxip1 pre-B-cell leukemia transcription factor interacting protein 1 0.04 1.22 Olig2 oligodendrocyte transcription factor 2 0.049 1.22 Adcy5 adenylate cyclase 5 0.01 1.23 Ap3b2 adaptor-related protein complex 3, beta 2 subunit 0.036 1.23 APP regulates male sexual behavior 9

2610301K12Rik RIKEN cDNA 2610301K12 gene 0.04 1.23 Trak1 trafficking protein, kinesin binding 1 0.042 1.23 Trim35 tripartite motif-containing 35 0.043 1.23 Rai17 retinoic acid induced 17 0.045 1.23 B930006L02Rik RIKEN cDNA B930006L02 gene 0.047 1.23 Egln1 EGL nine homolog 1 (C. elegans) 0.015 1.24 Usp48 ubiquitin specific peptidase 48 0.022 1.24 1810013L24Rik RIKEN cDNA 1810013L24 gene 0.026 1.24 1700012G19Rik RIKEN cDNA 1700012G19 gene 0.036 1.24 Robo1 roundabout homolog 1 (Drosophila) 0.039 1.24 Zfand3 zinc finger, AN1-type domain 3 0.041 1.24 Sema6d sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D 0.018 1.25 Fem1c fem-1 homolog c (C.elegans) 0.025 1.25 Phf21a PHD finger protein 21A 0.025 1.25 Atp9a ATPase, class II, type 9A 0.027 1.25 Camta1 calmodulin binding transcription activator 1 0.03 1.25 9630007J19Rik RIKEN cDNA 9630007J19 gene 0.036 1.25 Usp14 ubiquitin specific peptidase 14 0.039 1.25 Mtmr3 myotubularin related protein 3 0.041 1.25 Elmo2 engulfment and cell motility 2, ced-12 homolog (C. elegans) 0.041 1.25 Ppp1r12b protein phosphatase 1, regulatory (inhibitor) subunit 12B 0.031 1.26 Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) 0.036 1.26 Mapk8ip2 mitogen-activated protein kinase 8 interacting protein 2 0.04 1.26 6430548M08Rik RIKEN cDNA 6430548M08 gene 0.01 1.27 Usp20 ubiquitin specific peptidase 20 0.013 1.27 Fxr2h fragile X mental retardation gene 2, autosomal homolog 0.022 1.27 Dopey2 dopey family member 2 0.029 1.27 Tns1 tensin 1 0.036 1.27 Tmem38a transmembrane protein 38a 0.038 1.27 Tbc1d10b TBC1 domain family, member 10b 0.041 1.27 Psmd11 proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 0.044 1.27 Snx27 sorting nexin family member 27 0.046 1.27 Phf17 PHD finger protein 17 0.048 1.27 Traf3 Tnf receptor-associated factor 3 0.05 1.27 Kpnb1 karyopherin (importin) beta 1 0.012 1.27 Rab11fip4 RAB11 family interacting protein 4 (class II) 0.014 1.27 Eif4g1 eukaryotic translation initiation factor 4, gamma 1 0.022 1.27 4121402D02Rik RIKEN cDNA 4121402D02 gene 0.036 1.27 Iqsec1 IQ motif and Sec7 domain 1 0.042 1.27 BC011467 cDNA sequence BC011467 0.043 1.27 Ppfia4 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4 0.044 1.27 Kif1b kinesin family member 1B 0.045 1.27 Snag1 sorting nexin associated golgi protein 1 0.046 1.27 Nrxn3 neurexin III 0.048 1.27 Ntrk3 neurotrophic tyrosine kinase, receptor, type 3 0.036 1.28 Ube4b ubiquitination factor E4B, UFD2 homolog (S. cerevisiae) 0.037 1.28 Fgfr1 fibroblast growth factor receptor 1 0.04 1.28 Ankrd17 ankyrin repeat domain 17 0.05 1.28 B230380D07Rik RIKEN cDNA B230380D07 gene 0.01 1.29 1200009O22Rik RIKEN cDNA 1200009O22 gene 0.019 1.29 APP regulates male sexual behavior 10

Ep400 E1A binding protein p400 0.022 1.29 Ssr3 signal sequence receptor, gamma 0.038 1.29 Mtap4 microtubule-associated protein 4 0.045 1.29 Csk c-src tyrosine kinase 0.048 1.29 Nfib /B 0.048 1.29 Ppp2r4 protein phosphatase 2A, regulatory subunit B (PR 53) 0.048 1.29 A230069A22Rik RIKEN cDNA A230069A22 gene 0.05 1.29 Usf2 upstream transcription factor 2 0.013 1.3 2310047A01Rik RIKEN cDNA 2310047A01 gene 0.034 1.3 Gna13 guanine nucleotide binding protein, alpha 13 0.036 1.3 Clstn1 calsyntenin 1 0.038 1.3 Atp8b2 Atpase, class I, type 8B, member 2 0.04 1.3 Poldip3 polymerase (DNA-directed), delta interacting protein 3 0.04 1.3 6330442E10Rik RIKEN cDNA 6330442E10 gene 0.044 1.3 5730453I16Rik RIKEN cDNA 5730453I16 gene 0.045 1.3 Cd8b1 CD8 antigen, beta chain 1 0.019 1.31 Bahd1 bromo adjacent homology domain containing 1 0.022 1.31 Ube2h ubiquitin-conjugating enzyme E2H 0.035 1.31 Trak2 trafficking protein, kinesin binding 2 0.04 1.31 2600011C06Rik RIKEN cDNA 2600011C06 gene 0.043 1.31 Sirpa signal-regulatory protein alpha 0.044 1.31 Pcnxl3 pecanex-like 3 (Drosophila) 0.045 1.31 Dpp9 dipeptidylpeptidase 9 0.045 1.31 Mark2 MAP/microtubule affinity-regulating kinase 2 0.018 1.32 Phactr1 phosphatase and actin regulator 1 0.021 1.32 Glcci1 glucocorticoid induced transcript 1 0.034 1.32 Wdr26 WD repeat domain 26 0.038 1.32 11-Sep septin 11 0.04 1.32 Arhgef10l Rho guanine nucleotide exchange factor (GEF) 10-like 0.04 1.32 Rxra alpha 0.047 1.32 Efnb3 ephrin B3 0.012 1.33 Ndst1 N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 1 0.029 1.33 Irak1 interleukin-1 receptor-associated kinase 1 0.037 1.33 Setd6 SET domain containing 6 0.038 1.33 Auts2 autism susceptibility candidate 2 0.042 1.33 Sall2 sal-like 2 (Drosophila) 0.044 1.33 Gm672 gene model 672, (NCBI) 0.045 1.33 Samd14 sterile alpha motif domain containing 14 0.047 1.33 Cacna1b calcium channel, voltage-dependent, N type, alpha 1B subunit 0.038 1.34 Trpm3 transient receptor potential cation channel, subfamily M, member 3 0.04 1.34 Kpnb1 karyopherin (importin) beta 1 0.04 1.34 Sez6l2 seizure related 6 homolog like 2 0.043 1.34 Dullard Dullard homolog (Xenopus laevis) 0.045 1.34 Ppp1r12c protein phosphatase 1, regulatory (inhibitor) subunit 12C 0.014 1.35 S100pbp S100P binding protein 0.035 1.35 Lrrc35 leucine rich repeat containing 35 0.036 1.35 Ube2h ubiquitin-conjugating enzyme E2H 0.037 1.35 Disp2 dispatched homolog 2 (Drosophila) 0.039 1.35 1700021K19Rik RIKEN cDNA 1700021K19 gene 0.044 1.35 Gnao1 guanine nucleotide binding protein, alpha o 0.014 1.36 APP regulates male sexual behavior 11

Dtx1 deltex 1 homolog (Drosophila) 0.018 1.36 Pcdhgc3 protocadherin gamma subfamily C, 3 0.029 1.36 6430511F03 0.032 1.36 Rap1gds1 RAP1, GTP-GDP dissociation stimulator 1 0.035 1.36 R3hdm1 R3H domain 1 (binds single-stranded nucleic acids) 0.036 1.36 Arl4c ADP-ribosylation factor-like 4C 0.047 1.36 Adora1 adenosine A1 receptor 0.047 1.36 Dzip1 DAZ interacting protein 1 0.029 1.37 Accn2 amiloride-sensitive cation channel 2, neuronal 0.036 1.37 Sin3a transcriptional regulator, SIN3A (yeast) 0.038 1.37 Dock3 dedicator of cyto-kinesis 3 0.04 1.37 Rere arginine glutamic acid dipeptide (RE) repeats 0.048 1.37 Tegt testis enhanced gene transcript 0.021 1.38 Lba1 lupus brain antigen 1 0.021 1.38 Gprin1 G protein-regulated inducer of neurite outgrowth 1 0.036 1.38 Glg1 golgi apparatus protein 1 0.038 1.38 4930461P20Rik RIKEN cDNA 4930461P20 gene 0.04 1.38 Dctn1 dynactin 1 0.041 1.38 Brd3 bromodomain containing 3 0.042 1.38 Acin1 apoptotic chromatin condensation inducer 1 0.044 1.38 Dot1l DOT1-like, histone H3 methyltransferase (S. cerevisiae) 0.046 1.38 Atp1a2 ATPase, Na+/K+ transporting, alpha 2 polypeptide 0.012 1.39 Ube2o ubiquitin-conjugating enzyme E2O 0.021 1.39 Sgpl1 sphingosine phosphate lyase 1 0.022 1.39 Zfp36l2 zinc finger protein 36, C3H type-like 2 0.033 1.39 Cpeb4 cytoplasmic polyadenylation element binding protein 4 0.043 1.39 Foxj2 forkhead box J2 0.026 1.39 Zfp740 zinc finger protein 740 0.029 1.39 Snx30 sorting nexin family member 30 0.036 1.39 Nrp2 neuropilin 2 0.042 1.39 Ywhae tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 0.044 1.39 D030051N19Rik RIKEN cDNA D030051N19 gene 0.01 1.4 Arl8a ADP-ribosylation factor-like 8A 0.029 1.4 Pex6 peroxisomal biogenesis factor 6 0.039 1.4 Ubn1 ubinuclein 1 0.04 1.4 Pitpnm1 phosphatidylinositol membrane-associated 1 0.048 1.4 Stox2 storkhead box 2 0.014 1.41 Mapt microtubule-associated protein tau 0.015 1.41 Rprc1 arginine/proline rich coiled-coil 1 0.031 1.41 Mtap1a microtubule-associated protein 1 A 0.036 1.41 Sin3b transcriptional regulator, SIN3B (yeast) 0.043 1.41 Pafah1b1 platelet-activating factor acetylhydrolase, isoform 1b, beta1 subunit 0.05 1.41 9430020K01Rik RIKEN cDNA 9430020K01 gene 0.041 1.42 Tnrc6b trinucleotide repeat containing 6b 0.023 1.43 1110061N23Rik RIKEN cDNA 1110061N23 gene 0.04 1.43 Vangl2 vang-like 2 (van gogh, Drosophila) 0.05 1.43 AI840826 expressed sequence AI840826 0.01 1.44 Sf1 splicing factor 1 0.025 1.44 Slc9a5 solute carrier family 9 (sodium/hydrogen exchanger), member 5 0.028 1.44 Ddr1 discoidin domain receptor family, member 1 0.028 1.44 APP regulates male sexual behavior 12

Foxk2 forkhead box K2 0.038 1.44 D17Wsu92e DNA segment, Chr 17, Wayne State University 92, expressed 0.04 1.44 E030004N02Rik RIKEN cDNA E030004N02 gene 0.04 1.44 Ywhae tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide 0.05 1.44 Pcm1 pericentriolar material 1 0.01 1.45 Mapt microtubule-associated protein tau 0.021 1.45 Ctdsp1 CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1 0.04 1.45 Freq frequenin homolog (Drosophila) 0.04 1.45 C230093N12Rik RIKEN cDNA C230093N12 gene 0.044 1.45 Gabpb2 GA repeat binding protein, beta 2 0.045 1.45 Nmnat2 nicotinamide nucleotide adenylyltransferase 2 0.045 1.45 Nbeal1 neurobeachin like 1 0.046 1.45 Rims3 regulating synaptic membrane exocytosis 3 0.046 1.45 Casc3 cancer susceptibility candidate 3 0.012 1.46 Acvr2b activin receptor IIB 0.028 1.46 Mamdc1 MAM domain containing 1 0.048 1.46 Nlgn2 neuroligin 2 0.021 1.47 Grik5 glutamate receptor, ionotropic, kainate 5 (gamma 2) 0.031 1.47 Atp1a2 ATPase, Na+/K+ transporting, alpha 2 polypeptide 0.032 1.47 Podxl2 podocalyxin-like 2 0.032 1.47 AI840826 expressed sequence AI840826 0.037 1.47 Hcfc1 host cell factor C1 0.014 1.48 Smarcc2 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2 0.018 1.48 Slc36a1 solute carrier family 36 (proton/ symporter), member 1 0.041 1.48 Supt6h suppressor of Ty 6 homolog (S. cerevisiae) 0.043 1.48 Gspt1 G1 to S phase transition 1 0.047 1.48 LOC381742 0.027 1.49 Znrf1 zinc and ring finger 1 0.032 1.49 Mta1 metastasis associated 1 0.036 1.49 Taf4a TAF4A RNA polymerase II, TATA box binding protein (TBP)-associated factor 0.019 1.51 Lonrf2 LON peptidase N-terminal domain and ring finger 2 0.025 1.51 Map2k7 mitogen activated protein kinase kinase 7 0.045 1.51 Pacsin1 protein kinase C and casein kinase substrate in neurons 1 0.05 1.51 Cttnbp2 cortactin binding protein 2 0.044 1.52 Ubqln4 ubiquilin 4 0.022 1.53 Rgmb RGM domain family, member B 0.022 1.53 Tmcc2 transmembrane and coiled-coil domains 2 0.025 1.53 1500016O10Rik RIKEN cDNA 1500016O10 gene 0.041 1.53 Spred1 sprouty protein with EVH-1 domain 1, related sequence 0.043 1.54 Bag3 Bcl2-associated athanogene 3 0.014 1.56 4833411O04Rik RIKEN cDNA 4833411O04 gene 0.027 1.58 Vamp2 vesicle-associated membrane protein 2 0.04 1.58 Palm paralemmin 0.042 1.58 Tmcc2 transmembrane and coiled-coil domains 2 0.01 1.59 Pacs2 phosphofurin acidic cluster sorting protein 2 0.047 1.59 Rusc2 RUN and SH3 domain containing 2 0.013 1.61 Mtap4 microtubule-associated protein 4 0.028 1.61 Gabpb1 GA repeat binding protein, beta 1 0.037 1.64 Cnp1 cyclic nucleotide phosphodiesterase 1 0.05 1.64 Elavl3 ELAV (embryonic lethal, abnormal vision, Drosophila)-like 3 (Hu antigen C) 0.043 1.66 APP regulates male sexual behavior 13

Ahdc1 AT hook, DNA binding motif, containing 1 0.047 1.66 Gatad2a GATA zinc finger domain containing 2A 0.04 1.67 Spred1 sprouty protein with EVH-1 domain 1, related sequence 0.021 1.68 Vat1 vesicle amine transport protein 1 homolog (T californica) 0.025 1.69 Aatk -associated tyrosine kinase 0.043 1.69 Mapre3 microtubule-associated protein, RP/EB family, member 3 0.026 1.71 Syp synaptophysin 0.031 1.73 Centg2 centaurin, gamma 2 0.045 1.73 Odz4 odd Oz/ten-m homolog 4 (Drosophila) 0.045 1.73 Vat1 vesicle amine transport protein 1 homolog (T californica) 0.04 1.75 Spock2 sparc/osteonectin, cwcv and kazal-like domains proteoglycan 2 0.044 1.77 Vasp vasodilator-stimulated phosphoprotein 0.04 1.96 Pde4a phosphodiesterase 4A, cAMP specific 0.045 1.99 Hipk2 homeodomain interacting protein kinase 2 0.01 2.19 Pum2 pumilio 2 (Drosophila) 0.04 2.31 5730405I09Rik RIKEN cDNA 5730405I09 gene 0.038 2.53

APP regulates male sexual behavior 14

Supplemental Table 2. Candidate genes derived from two recombinant strains of the hybrid B6D2F1 mice that displayed mating after orchidectomy (BXD25 and BXD28) and four recombinant strains that ceased mating after orchidectomy (BXD1, BXD2, BXD30, and BXD31) as detailed in a previous report

(Coquelin, 1991). Listed are the chromosome, , Mb, and gene (derived utilizing the latest mouse genome assembly, Mouse Build 37), of each allele derived either from the C57BL/6J female (B) or the

DBA/2J male (D) in which the alleles were identical among the two mater strains but different from those in the four non-mating strains. Data were derived from the BXD genotype file from The

GeneNetwork, (http://www.genenetwork.org/dbdoc/BXDGeno.html).

Supplemental Table 2 Chr Locus Mb BXD25 BXD28 BXD1 BXD2 BXD30 BXD31 Gene 2 rs13476520 57.934897 B B D D D D Galnt5 2 rs13476521 58.056566 B B D D D D 2 rs3672719 58.115109 B B D D D D 2 rs13476525 58.821825 B B D D D D BC062650 2 rs3660779 60.503727 B B D D D D Itgb6 2 rs13476533 60.957071 B B D D D D 2 rs3688501 61.795549 B B D D D D 2 rs6318340 62.017766 B B D D D D Slc4a10 2 rs3022886 62.500734 B B D D D D Ifih1 2 rs3709716 62.507709 B B D D D D 2 rs13476538 62.735038 B B D D D D Kcnh7 2 rs6391732 62.858408 B B D D D D Kcnh7 CEL- 2 2_63291612 63.208377 B B D D D D 2 rs13476540 63.428253 B B D D D D 2 rs3664317 63.704557 B B D D D D 2 rs6314726 63.968243 B B D D D D 2 rs3676478 64.597379 B B D D D D 2 rs6302554 64.714013 B B D D D D 2 rs6222797 64.974452 B B D D D D Cobll1 2 D2Mit205 65.157324 B B D D D D 2 rs13476548 65.513895 B B D D D D 2 gnf02.064.546 65.721616 B B D D D D 2 rs13476551 66.088937 B B D D D D Ttc21b 2 rs3715385 66.205552 B B D D D D Scn1a 2 rs3723010 66.356522 B B D D D D Scn9a 2 rs13476553 66.823301 B B D D D D 2 rs6275559 69.580005 B B D D D D Ppig 2 rs4223211 69.652245 B B D D D D Phospho2 2 mCV25429456 69.791162 B B D D D D APP regulates male sexual behavior 15

2 rs6165818 70.094861 B B D D D D 2 rs13476571 72.099544 B B D D D D B230120H23Rik 2 rs13476573 72.992803 B B D D D D 1700023B02Rik CEL- 2 2_73370728 73.174311 B B D D D D 2 rs6367022 73.772974 B B D D D D 2 rs3689263 74.008873 B B D D D D 2 rs3719468 74.787396 B B D D D D 2 rs13459064 75.374482 B B D D D D Nfe2l2 2 rs13476889 163.764495 B B D D D D 2 rs3696248 163.814773 B B D D D D Slpi 2 rs8275857 163.83093 B B D D D D 2 rs3675564 163.847131 B B D D D D Matn4 2 rs13476891 164.19099 B B D D D D Wfdc3 2 rs6185704 164.410259 B B D D D D Mmp9 3 rs13476950 3.750239 B B D D D D 3 rs13476958 5.879032 B B D D D D 3 gnf03.003.469 6.380311 B B D D D D 3 rs13476961 6.788035 B B D D D D 3 rs8246984 7.589343 B B D D D D 3 rs13476963 7.654388 B B D D D D 3 rs13476966 8.741932 B B D D D D 3 rs3668064 9.257087 B B D D D D Zbtb10 3 rs3701904 9.481092 B B D D D D Zfp704 3 rs13476970 10.01795 B B D D D D 3 rs6405415 10.32865 B B D D D D Impa1 3 rs13476973 11.016045 B B D D D D 3 rs6351478 12.567 B B D D D D 3 rs3674446 13.758422 B B D D D D 0710005M24Rik 3 rs6398851 14.37497 B B D D D D 3 rs13476986 15.210333 B B D D D D 3 rs13476987 15.333199 B B D D D D 3 rs3659988 15.789917 B B D D D D 3 rs13476993 16.408474 B B D D D D 3 rs6345660 16.501296 B B D D D D 3 mCV22614242 16.965115 B B D D D D 3 rs3696263 17.202556 B B D D D D 3 mCV24712981 17.904493 B B D D D D 3 rs13476999 18.062806 B B D D D D 3 mCV24211562 18.600662 B B D D D D 3 rs13477004 18.986563 B B D D D D Trim55 3 rs13477011 20.707073 B B D D D D 3 rs13477017 21.965434 B B D D D D CEL- 3 3_23204282 22.857404 B B D D D D 3 rs6373308 25.611098 B B D D D D Nlgn1 3 rs13477026 25.79816 B B D D D D similar to cullin 2 similar to PRAME family 5 rs13478398 93.061377 B B D D D D member 9 CEL- 5 5_93652588 95.358813 B B D D D D 5 D5Mit312 95.409921 B B D D D D Fras1 5 CEL- 95.501418 B B D D D D APP regulates male sexual behavior 16

5_93794990 CEL- 5 5_93945748 95.656924 B B D D D D 5 rs3697719 96.064498 B B D D D D Bmp2k 5 rs4225380 96.400586 B B D D D D C80008 5 rs3706737 96.528453 B B D D D D 12 rs13481282 5.166043 B B D D D D 12 rs13481285 6.253897 B B D D D D 13 rs13481874 69.022856 B B D D D D 13 rs3664096 71.822131 B B D D D D Ell2 13 rs3688040 74.183229 B B D D D D 1110033M05Rik 13 rs13481896 74.339459 B B D D D D 13 rs3702220 76.818867 B B D D D D 13 rs3722797 76.999078 B B D D D D 18 rs13483314 40.167873 B B D D D D 18 rs6313313 41.881959 B B D D D D 18 rs3683264 42.001744 B B D D D D 18 rs13483326 42.969414 B B D D D D Ppp2r2b 18 rs3676196 43.14444 B B D D D D Ppp2r2b 18 rs13483328 43.374657 B B D D D D 18 D18Mit149 45.216955 B B D D D D 19 D19Msw029 28.469275 B B D D D D 19 rs13483589 29.233782 B B D D D D 19 mCV24505422 29.917354 B B D D D D 19 rs13483591 30.066798 B B D D D D Prkg1 1 rs6269442 3.451386 D D B B B B Xkr4 1 rs6365999 4.781375 D D B B B B Lypla1 1 rs6376963 4.978402 D D B B B B Rgs20 1 rs3677817 5.149339 D D B B B B 1 rs8236463 5.551627 D D B B B B Oprk1 1 rs6333200 6.193353 D D B B B B Rb1cc1 1 rs6298633 6.795759 D D B B B B St18 1 rs6241531 10.11096 D D B B B B 4930418G15Rik 1 rs6360236 11.189287 D D B B B B 1 rs3722996 11.374815 D D B B B B C030045D06Rik 1 D1Mit1 11.62114 D D B B B B A830018L16Rik 1 D1Mit294 11.864015 D D B B B B A830018L16Rik 1 rs13475728 12.849916 D D B B B B Sulf1 6 rs6308025 55.314999 D D B B B B Inmt 6 rs3687179 55.662338 D D B B B B CEL- 6 6_56528034 56.712722 D D B B B B CEL- 6 6_57082524 57.267712 D D B B B B 6 rs13478780 59.767028 D D B B B B 6 gnf06.058.959 60.050421 D D B B B B 6 rs13478783 60.831469 D D B B B B 7 rs13479441 98.26138 D D B B B B Ubqlnl 7 rs3662937 98.345334 D D B B B B Trim6 7 rs13479443 98.708913 D D B B B B Olfr655 CEL- 7 7_92439740 99.025716 D D B B B B 7 rs13459173 99.751214 D D B B B B Arfip2 APP regulates male sexual behavior 17

7 rs13479447 99.877595 D D B B B B Dchs1 7 rs3711721 101.175165 D D B B B B 7 rs13479451 101.318177 D D B B B B 7 rs3724540 101.348201 D D B B B B Syt9 7 rs6386601 101.552854 D D B B B B Ppfibp2 7 rs3686975 103.832616 D D B B B B Tmem41b 7 rs13479460 105.527967 D D B B B B Galntl4 7 rs3726501 105.681513 D D B B B B 7 rs3679005 106.176779 D D B B B B Mical2 7 rs3674465 106.589554 D D B B B B Tead1 7 D7Mit222 106.788207 D D B B B B 7 rs6280792 106.905182 D D B B B B 7 rs3691339 107.405713 D D B B B B Mlstd2 7 rs3657482 107.921002 D D B B B B Rras2 7 rs6194926 108.222953 D D B B B B 4933406I18Rik 12 rs13481566 81.400219 D D B B B B 16 rs4217061 89.042706 D D B B B B Tiam1 16 rs4217722 89.767027 D D B B B B 16 rs4219239 90.827329 D D B B B B Son 16 rs4219613 91.571128 D D B B B B 16 rs4219897 91.955638 D D B B B B 16 rs4219905 92.164976 D D B B B B 16 rs3712360 92.703714 D D B B B B 16 rs6389547 92.849917 D D B B B B Cbr3 16 rs4220193 93.026631 D D B B B B Morc3 16 rs6375622 93.105169 D D B B B B

Coquelin, A. (1991). Persistent sexual behavior in castrated, recombinant inbred mice. Biol Reprod 45, 680-684.

APP regulates male sexual behavior 18

Supplemental Table 3. Forward and reverse nucleotide sequences of MAPT, APP, PTEN,

SCN1a, IMPA1, SOD1 and β-actin primers.

Gene Accession # Nucleotide sequence

MAPT NM_010838 Forward 5’-TGGACCTGAGCAAAGTGACCT

Reverse 5’-TTTTACTTCCACCTGGCCACC

APP NM_007471 Forward 5’-AAACGACGCTCTCATGCCTTC

Reverse 5’-CAGGCTGAATTCCCCATTCAC

PTEN NM_008960 Forward 5’-TGCCACAAAGTGCCTCGTTT

Reverse 5’-CCGTTTAGTGCACAGATCCACC

SCN1a NM_018733 Forward 5’-TGTTAAGCATTCGTGGTTCCCT

Reverse 5’-GCTCGCCCTCTAAAGCTGAAA

IMPA1 NM_018864 Forward 5’-AGCTGTTTCAATTGGCTTCCTT

Reverse 5’-GCCGGTGTACATCTTATCTTCCA

SOD1 NM_011434 Forward 5’-GGGATTGCGCAGTAAACATTC

Reverse 5’-AATGGTTTGAGGGTAGCAGATGA

β-actin NM_007393 Forward 5’-CCAGATCATGTTTGAGACCTTCAA

Reverse 5’-CCAGAGGCGTACAGGGATAGC