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Supplemental Material APP regulates male sexual behavior 1 Supplemental Figure 1. Principle components analysis of the maters (red diamonds labeled M) versus non-maters (black circles labeled C) where each point represents a intensity data from one microarray hybridization. The two principle components axes, PC1 and PC2, represent directions associated with the highest and the second highest degree of variability in the data of all ~40,000 genes on the array. APP regulates male sexual behavior 2 Supplemental Table 1. 532 differentially expressed genes after applying a 5% False Discovery Rate cutoff between maters and non-maters (n=5/group). Listed are the gene symbols, description, p-value and fold difference. Supplemental Table 1 p- Fold Gene Symbol Description value ∆ Pabpc1 poly A binding protein, cytoplasmic 1 0.036 2.62 D630023F18Rik RIKEN cDNA D630023F18 gene 0.036 1.78 Ccdc82 coiled-coil domain containing 82 0.015 1.66 Cdt1 chromatin licensing and DNA replication factor 1 0.021 1.65 Mmrn2 multimerin 2 0.035 1.62 Efcbp1 EF hand calcium binding protein 1 0.04 1.61 Rrm1 ribonucleotide reductase M1 0.042 1.59 Cox7c cytochrome c oxidase, subunit VIIc 0.04 1.57 Bdnf brain derived neurotrophic factor 0.034 1.52 Sox11 SRY-box containing gene 11 0.048 1.52 Srr serine racemase 0.012 1.49 Zfp291 zinc finger protein 291 0.038 1.49 Setmar SET domain and mariner transposase fusion gene 0.043 1.49 AI462438 expressed sequence AI462438 0.023 1.48 Zipro1 zinc finger proliferation 1 0.044 1.47 Suv39h1 suppressor of variegation 3-9 homolog 1 (Drosophila) 0.022 1.45 Zfp62 zinc finger protein 62 0.048 1.45 1110057K04Rik RIKEN cDNA 1110057K04 gene 0.037 1.44 Mak10 MAK10 homolog, amino-acid N-acetyltransferase subunit, (S. cerevisiae) 0.021 1.43 Sap30bp SAP30 binding protein 0.036 1.43 Snip1 Smad nuclear interacting protein 1 0.04 1.42 Rbm18 RNA binding motif protein 18 0.037 1.41 Ehd1 EH-domain containing 1 0.047 1.4 1700052N19Rik RIKEN cDNA 1700052N19 gene 0.021 1.39 2700062C07Rik RIKEN cDNA 2700062C07 gene 0.023 1.39 Serf1 small EDRK-rich factor 1 0.047 1.39 1700034M03Rik RIKEN cDNA 1700034M03 gene 0.031 1.39 Cntn1 contactin 1 0.036 1.39 Epha4 Eph receptor A4 0.043 1.39 Rab39b RAB39B, member RAS oncogene family 0.043 1.38 LOC381255 0.046 1.38 Trim2 tripartite motif protein 2 0.047 1.37 BC066028 cDNA sequence BC066028 0.036 1.36 1110020G09Rik RIKEN cDNA 1110020G09 gene 0.036 1.36 Cryzl1 crystallin, zeta (quinone reductase)-like 1 0.046 1.36 Gnpda2 glucosamine-6-phosphate deaminase 2 0.049 1.36 Mtap methylthioadenosine phosphorylase 0.038 1.35 Josd3 Josephin domain containing 3 0.039 1.35 Arf2 ADP-ribosylation factor 2 0.043 1.35 Ankrd45 ankyrin repeat domain 45 0.049 1.35 Ints12 integrator complex subunit 12 0.041 1.34 Cml1 camello-like 1 0.041 1.34 Tatdn1 TatD DNase domain containing 1 0.044 1.34 APP regulates male sexual behavior 3 Pten phosphatase and tensin homolog 0.021 1.33 Kbtbd3 kelch repeat and BTB (POZ) domain containing 3 0.032 1.33 Lgtn ligatin 0.041 1.33 Ttc32 tetratricopeptide repeat domain 32 0.042 1.33 Epha4 Eph receptor A4 0.045 1.33 F2r coagulation factor II (thrombin) receptor 0.045 1.33 Mnat1 menage a trois 1 0.045 1.33 Btbd5 BTB (POZ) domain containing 5 0.021 1.32 Sgcb sarcoglycan, beta (dystrophin-associated glycoprotein) 0.038 1.32 C730024G19Rik RIKEN cDNA C730024G19 gene 0.041 1.32 Hist3h2a histone 3, H2a 0.044 1.32 Lnx1 ligand of numb-protein X 1 0.048 1.32 2900057D21Rik RIKEN cDNA 2900057D21 gene 0.01 1.31 Arpp19 cAMP-regulated phosphoprotein 19 0.015 1.31 Lgi1 leucine-rich repeat LGI family, member 1 0.022 1.31 4932415D10Rik RIKEN cDNA 4932415D10 gene 0.029 1.31 H2afj H2A histone family, member J 0.04 1.31 Sod1 superoxide dismutase 1, soluble 0.012 1.3 Stau2 staufen (RNA binding protein) homolog 2 (Drosophila) 0.019 1.29 AI256775 expressed sequence AI256775 0.036 1.29 Bxdc5 brix domain containing 5 0.045 1.29 Ufm1 ubiquitin-fold modifier 1 0.05 1.29 Ppp2r3a protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha 0.05 1.29 Lztfl1 leucine zipper transcription factor-like 1 0.022 1.28 LOC208595 0.025 1.28 Idh3a isocitrate dehydrogenase 3 (NAD+) alpha 0.029 1.28 Folh1 folate hydrolase 0.032 1.28 Arg2 arginase type II 0.036 1.28 Fads1 fatty acid desaturase 1 0.038 1.28 Scn1a sodium channel, voltage-gated, type I, alpha 0.038 1.28 Exosc1 exosome component 1 0.04 1.28 Trappc5 trafficking protein particle complex 5 0.04 1.28 Trfr2 transferrin receptor 2 0.049 1.28 Nme5 expressed in non-metastatic cells 5 0.05 1.28 1110034B05Rik RIKEN cDNA 1110034B05 gene 0.05 1.28 Pgm3 phosphoglucomutase 3 0.013 1.27 Cryzl1 crystallin, zeta (quinone reductase)-like 1 0.015 1.27 Tmem167 transmembrane protein 167 0.015 1.27 Wdr61 WD repeat domain 61 0.021 1.27 Dpm1 dolichol-phosphate (beta-D) mannosyltransferase 1 0.026 1.27 Ppil3 peptidylprolyl isomerase (cyclophilin)-like 3 0.036 1.27 Scfd1 sec1 family domain containing 1 0.019 1.27 4933433P14Rik RIKEN cDNA 4933433P14 gene 0.029 1.27 Orc3l origin recognition complex, subunit 3-like (S. cerevisiae) 0.029 1.27 Anapc10 anaphase promoting complex subunit 10 0.036 1.27 Gtf2b general transcription factor IIB 0.042 1.27 Tmem167 transmembrane protein 167 0.014 1.26 1810030O07Rik RIKEN cDNA 1810030O07 gene 0.024 1.26 Rage renal tumor antigen 0.025 1.26 2310045N01Rik RIKEN cDNA 2310045N01 gene 0.028 1.26 APP regulates male sexual behavior 4 H2afz H2A histone family, member Z 0.04 1.26 Arfrp1 ADP-ribosylation factor related protein 1 0.044 1.26 Gtdc1 glycosyltransferase-like domain containing 1 0.046 1.26 Zfp706 zinc finger protein 706 0.047 1.26 Ergic2 ERGIC and golgi 2 0.01 1.25 Mrpl21 mitochondrial ribosomal protein L21 0.021 1.25 Exosc8 exosome component 8 0.032 1.25 Ube1c ubiquitin-activating enzyme E1C 0.034 1.25 Rnf121 ring finger protein 121 0.036 1.25 Mpp5 membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) 0.038 1.25 Stk38l serine/threonine kinase 38 like 0.042 1.25 Nup88 nucleoporin 88 0.042 1.25 Zfp273 zinc finger protein 273 0.043 1.25 Tnrc15 trinucleotide repeat containing 15 0.047 1.25 Polr3k polymerase (RNA) III (DNA directed) polypeptide K 0.048 1.25 1110059P08Rik RIKEN cDNA 1110059P08 gene 0.05 1.25 Zfp46 zinc finger protein 46 0.019 1.24 Rpl7l1 ribosomal protein L7-like 1 0.021 1.24 Ociad2 OCIA domain containing 2 0.021 1.24 Snord22 small nucleolar RNA, C/D box 22 0.027 1.24 Trnt1 tRNA nucleotidyl transferase, CCA-adding, 1 0.035 1.24 Blzf1 basic leucine zipper nuclear factor 1 0.04 1.24 Hars histidyl-tRNA synthetase 0.04 1.24 Cdadc1 cytidine and dCMP deaminase domain containing 1 0.04 1.24 Uros uroporphyrinogen III synthase 0.045 1.24 Mrpl3 mitochondrial ribosomal protein L3 0.045 1.24 0610038F07Rik RIKEN cDNA 0610038F07 gene 0.013 1.23 Anapc10 anaphase promoting complex subunit 10 0.021 1.23 Mrpl48 mitochondrial ribosomal protein L48 0.022 1.23 A230046K03Rik RIKEN cDNA A230046K03 gene 0.023 1.23 Mrpl49 mitochondrial ribosomal protein L49 0.025 1.23 Eif3s6 eukaryotic translation initiation factor 3, subunit 6 0.029 1.23 0610038F07Rik RIKEN cDNA 0610038F07 gene 0.031 1.23 Mak10 MAK10 homolog, amino-acid N-acetyltransferase subunit, (S. cerevisiae) 0.036 1.23 Rg9mtd3 RNA (guanine-9-) methyltransferase domain containing 3 0.038 1.23 Igsf1 immunoglobulin superfamily, member 1 0.04 1.23 Eif2s1 eukaryotic translation initiation factor 2, subunit 1 alpha 0.04 1.23 Mtg1 mitochondrial GTPase 1 homolog (S. cerevisiae) 0.04 1.23 Sugt1 SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) 0.042 1.23 Pigf phosphatidylinositol glycan anchor biosynthesis, class F 0.045 1.23 Rabl4 RAB, member of RAS oncogene family-like 4 0.047 1.23 Snupn snurportin 1 0.048 1.23 BC011248 cDNA sequence BC011248 0.049 1.23 C130086A10 0.01 1.22 Acn9 ACN9 homolog (S. cerevisiae) 0.028 1.22 Creb3 cAMP responsive element binding protein 3 0.03 1.22 1700026B20Rik RIKEN cDNA 1700026B20 gene 0.036 1.22 Cdc5l cell division cycle 5-like (S. pombe) 0.038 1.22 Man1a2 mannosidase, alpha, class 1A, member 2 0.041 1.22 Kpna2 karyopherin (importin) alpha 2 0.042 1.22 APP regulates male sexual behavior 5 Tmem128 transmembrane protein 128 0.048 1.22 Coq3 coenzyme Q3 homolog, methyltransferase (yeast) 0.01 1.21 Pigx phosphatidylinositol glycan anchor biosynthesis, class X 0.022 1.21 1500011J06Rik RIKEN cDNA 1500011J06 gene 0.026 1.21 Mrps33 mitochondrial ribosomal protein S33 0.037 1.21 Jagn1 jagunal homolog 1 (Drosophila) 0.037 1.21 Commd6 COMM domain containing 6 0.038 1.21 Ssbp1 single-stranded DNA binding protein 1 0.044 1.21 Gpr137b G protein-coupled receptor 137B 0.046 1.21 2610029G23Rik RIKEN cDNA 2610029G23 gene 0.028 1.21 Hirip5 histone cell cycle regulation defective interacting protein 5 0.037 1.21 Mrpl35 mitochondrial ribosomal protein L35 0.04 1.21 Bdh1 3-hydroxybutyrate dehydrogenase, type 1 0.042 1.21 Ddx52 DEAD (Asp-Glu-Ala-Asp) box polypeptide 52 0.043 1.21 Tbc1d15 TBC1 domain family, member 15 0.043 1.21 BC023829 cDNA sequence BC023829 0.043 1.21 Ccdc32 coiled-coil domain containing 32 0.044 1.21 Asns asparagine synthetase 0.045 1.21 Immp1l IMP1 inner mitochondrial membrane peptidase-like (S.
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