Catalog # Aliquot Size

U221-911-05 3 x 5 nmol U221-911-20 3 x 20 nmol U221-911-50 3 x 50 nmol

UBE2G1 (UBC7) siRNA Set I siRNA duplexes targeted against three exon regions

Catalog # U221-911

Lot # Z2109-23

Specificity Formulation UBE2G1 (UBC7) siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human UBE2G1 (UBC7) expression. shipped at room temperature.

Product Description Reconstitution Protocol UBE2G1 (UBC7) siRNA is a pool of three individual Briefly centrifuge the tubes (maximum RCF 4,000g) to synthetic siRNA duplexes designed to knock-down collect lyophilized siRNA at the bottom of the tube. human UBE2G1 (UBC7) mRNA expression. Each siRNA is Resuspend the siRNA in 50 µl of DEPC-treated water 19-25 bases in length. The accession number is (supplied by researcher), which results in a 1x stock NM_003342. solution (10 µM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot Gene Aliases 1x stock solutions for storage. UBC7; E217K; UBE2G Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the UBE2E3 (UBCH9) U219-30H lyophilized and resuspended siRNAs are stored at or UBE2F Protein U220-30H below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2G1 (UBC7) Protein U221-30H µM can undergo up to 50 freeze-thaw cycles without UBE2H Protein U223-30H significant degradation. For long-term storage, it is UBE2I Protein U224-30H recommended that the siRNA is stored at -70oC. For most UBE2J1 Protein U225-30G favorable performance, avoid repeated handling and UBE2K (UBC1) Protein U227-30H multiple freeze/thaw cycles. UBE2L6 (UBCH8) Protein U229-30H

Scientific Background UBE2G1 (UBC7) or -conjugating E2G 1 is a member of the E2 ubiquitin-conjugating enzyme family UBE2G1 (UBC7) siRNA Set I and catalyzes the covalent attachment of ubiquitin to siRNA duplexes targeted against three exon regions other which are involved in degradation of muscle-specific proteins. UBC7 is highly expressed in Catalog # U221-911 skeletal muscle (1). UBC7 is involved in the selective Lot # Z2109-23 ubiquitylation of different substrates by the same E3 Packaging Specifications 2.5 nmol/tube for 3 x 5nmol complex (2). Format Lyophilized powder Stability 1yr at -70oC from date of shipment References Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is 1. Watanabe, T. K. et.al: Molecular cloning of UBE2G, recommended that the lyophilized encoding a human skeletal muscle-specific ubiquitin- and resuspended siRNAs are stored at conjugating enzyme homologous to UBC7 of C. or below -20oC. After resuspension, elegans. Cytogenet. Cell Genet. 74: 146-148, 1996. siRNA stock solutions ≥2 µ M can undergo up to 50 freeze-thaw cycles 2. Shibata E. et.al: Selective ubiquitylation of p21 and without significant degradation. For Cdt1 by UBCH8 and UBE2G ubiquitin-conjugating long-term storage, it is recommended via the CRL4Cdt2 complex. that the siRNA is stored at -70oC. For most favorable performance, avoid Mol Cell Biol, 2011 Aug. repeated handling and multiple freeze/thaw cycles.

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FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS.