View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Digital.CSIC REBUILDING GLUTEN NETWORK OF DAMAGED WHEAT BY MEAN OF 2 GLUCOSE OXIDASE TREATMENT 4 A. Bonet1, C.M. Rosell1a, I. Pérez-Munuera2, I. Hernando2, 6 1 Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC). PO Box 73. Burjassot-46100. Valencia. Spain 8 2 Departamento de Tecnología de Alimentos, Universidad Politécnica de Valencia, P.O. 10 Box. 22012, 46071, Valencia, Spain. 12 a To whom correspondence should be addressed. E-mail:
[email protected] 14 Running title : Glucose oxidase effect on insect damaged gluten 16 - 1 - ABSTRACT 2 The disrupted gluten structure of infested wheat flours yields to low quality doughs unusable to bread-making processes. Enzymes are replacing chemical treatments in 4 the food industry as a tool to treat weak flours. Glucose oxidase (GO) is one of the most promising oxidative enzymes although it has not been demonstrated yet its 6 efficiency over the alcohol-soluble fraction of the gluten proteins. If this enzyme could restore the broken covalent bonds between the glutenin subunits, the gluten network of 8 damaged wheat flour would recover their native structure and functionality. This treatment would allow bakers to use damaged flour, reducing the economical losses 10 produced by this plague around Europe and North Africa. Electrophoretic studies demonstrated the formation of high molecular weight aggregates in the glutenin 12 fraction, which had a characteristic thermal stability depending on the enzyme dosage. Those molecular studies agreed with the bread-making assays made with the 14 maximum enzyme dosage and the microstructure determination.