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Tree Scale: 1 Ri AV19 Pyrococcus Yayanosi Orans 345-15

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Tree Scale: 1 Ri AV19 Pyrococcus Yayanosi Orans 345-15 Pyrococcus furiosus Thermococcus kodak Thermococcus onnur Thermococcus gamm Tree scale: 1 ri AV19 Pyrococcus yayanosi orans 345-15 Thermococcus sibiric Thermococcus sp. 45 ii OT3 Pyrococcus sp. ST04 E5 Methanothermus ferv Hrk 5 nsis IC-167 Methanocaldococcus Thermococcus sp. AM Thermococcus barop ta DSM 14429 vskia 768-28 ensis 768-20 arensis KOD1 atolerans EJ3 DSM 3638 Methanocaldococcus ineus NA1 Kra 1 Methanothermococcu Methanocaldococcus Methanopyrus kandle ntis JCM 11548 i CH1 Acidilobus saccharov icum DSM 13514 idus DSM 2088 us MM 739 se TE7 jannaschii DSM 2661 Methanocaldococcus 57 Pyrococcus horikosh Pyrococcus sp. NA2Pyrococcus abyssi G 0 hilus MP Thermofilum pendens Caldivirga maquilinge lum str. IM2 4 um DSM 4184 Methanocaldococcus VulcanisaetaVulcanisaeta distribu moutno s okinawensis sp. FS406-22 IH1 hilum V24Sta fervens AG86 Thermoproteus uzoni Thermoproteus tenax vulcanius M7 Pyrobaculum calidifo Candidatus Korarcha Methanotorris igneus PyrobaculumPyrobaculum arsenat ogunien Pyrobaculum sp. 186 infernus ME PyrobaculumPyrobaculum aerophi islandic Pyrobaculum neutrop eum cryptofilum OPF Aciduliprofundum bo Kol 5 Kam940 Ferroglobus placidus onei T469 8 W1 Archaeoglobus venef DSM 10642 Fervidicoccus fontis a DSM 5348 Archaeoglobus fulgid icus SNP6 na Ar-4 us DSM 4304 Acidianus hospitalis MetallosphaeraMetallosphaera sedul cupritr. 7 Archaeoglobus profundus DSM 5631 Sulfolobus tokodaii s arius DSM 639 Sulfolobus acidocald SulfolobusSulfolobus islandicussolfataricus P2 M.14.25 Ignicoccus hospitalis Aeropyrum pernix K1 KIN4/I Hydrogenobacter thermophilus TK-6 Pyrolobus fumarii 1A SM 14484 Hyperthermus butylic Thermocrinis albus D sp. YO3AOP1 Ignisphaera aggregan Sulfurihydrogenibium azorense Az-Fu1 Thermogladius cellul us DSM 5456 Aquifex aeolicus VF5 Staphylothermus mar Sulfurihydrogenibium Staphylothermus hell m DSM 6724 Thermosphaera aggre s DSM 17230 philum H-6-12 Desulfurococcus muc osum Rt17-B1 DesulfurococcusDesulfurococcus ferm kam olyticus 1633 Dictyoglomus turgidu EX-H1 5-2 inus F1 nivorans DSM 9078 enicus DSM 12710 Dictyoglomus thermo AmH gans DSM 11486 Fervidobacterium nod M16c01 Thermosipho africanu osus DSM 2162 Persephonella marina ThermotogaThermotoga naphtho neapolita chatkensis 1221n Fervidobacterium pen ThermotogaThermotoga sp. RQ2 petrophi Thermotoga maritima entans DSM 16532 Nitratiruptor sp. SB15 KC4 Caldanaerobacter sub Caldicellulosiruptor s SM 11699 Caldicellulosiruptor o Caldicellulosiruptor h Nautilia profundicola dicus DSM 15286 Caldicellulosiruptor k ificans HB-1 Caldisericum exile AZ m DSM 5069 Aquifex aeolicus VF5 s TCF52B ium geofontis OPF15 na DSM 4359 Ammonifex degensii phila RKU-10 la RKU-1 MSB8 ctoaceticus 6A escii DSM 6725 Thermodesulfatator in bsidiansis OB47 hermolithotrophum D accharolyticus DSMterraneus 8 subsp. teng ristjanssonii I77R1B wensensis OL ydrothermalis 108 ronotskyensis 2002 Thermovibrio ammon Thermotoga thermaru Thermodesulfobacter Caldicellulosiruptor la Caldicellulosiruptor b Desulfurobacterium t Caldicellulosiruptor o 903 congensis MB4 Caldicellulosiruptor k SI Fig. 1. Reproduction of RG phylogeny constructed by Weiss et al. 2016 (1) References 1. Weiss MC, et al. (2016) The physiology and habitat of the last universal common ancestor. Nat Microbiol 1:16116. 2. Tuteja, N., & Tuteja, R. (2004). Unraveling DNA helicases: motif, structure, mechanism and function. European journal of biochemistry 271(10), 1849-1863. 3. Jaxel, C., de la Tour, C. B., Duguet, M., & Nadal, M. (1996). Reverse gyrase gene from Sulfolobus shibatae B12: gene structure, transcription unit and comparative sequence analysis of the two domains. Nucleic acids research 24(23), 4668-4675. 2 .
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