Multiplex Diagnosis of Oncogenic Fusion and MET Exon Skipping by Molecular Counting Using Formalin-Fixed Paraffin Embedded Lung
ORIGINAL ARTICLE Multiplex Diagnosis of Oncogenic Fusion and MET Exon Skipping by Molecular Counting Using Formalin-Fixed Paraffin Embedded Lung Adenocarcinoma Tissues Kuniko Sunami, MD,a,g Koh Furuta, MD, PhD,b Koji Tsuta, MD, PhD,c Shinji Sasada, MD, PhD,d Takehiro Izumo, MD, PhD,d Takashi Nakaoku, MD,a Yoko Shimada, MFSc,a Motonobu Saito, MD, PhD,a Hiroshi Nokihara, MD, PhD,e Shun-ichi Watanabe, MD, PhD,f Yuichiro Ohe, MD, PhD,e,g Takashi Kohno, PhDa,* aDivision of Genome Biology, National Cancer Center Research Institute, Tokyo, Japan bDivision of Clinical Laboratory, National Cancer Center Hospital, Tokyo, Japan cDivision of Pathology, National Cancer Center Research Institute, Tokyo, Japan dDepartment of Endoscopy, Respiratory Endoscopy Division, National Cancer Center Research Institute, Tokyo, Japan eDepartment of Thoracic Oncology, National Cancer Center Research Institute, Tokyo, Japan fDepartment of Thoracic Surgery, National Cancer Center Hospital, Tokyo, Japan gCourse of Advanced Clinical Research of Cancer, Juntendo University Graduate School of Medicine, Tokyo, Japan Received 31 July 2015; revised 23 September 2015; accepted 13 October 2015 ABSTRACT detected oncogenic fusions in bronchial lavage fluid and transbronchial biopsy samples. Introduction: Fusions of the anaplastic lymphoma receptor tyrosine kinase gene (ALK), ret proto-oncogene (RET), ROS Conclusions: The MC assay allows multiplex detection of proto-oncogene 1, receptor tyrosine kinase gene (ROS1), B- oncogenic fusion and exon-skipped transcripts in tumor Raf proto-oncogene, serine/threonine kinase gene (BRAF), samples, including in formalin-fixed paraffin-embedded and neuregulin 1 gene (NRG1) and intronic MMNG HOS samples obtained in the clinic. Transforming gene (MET) mutations are druggable onco- Ó 2015 International Association for the Study of Lung gene alterations in lung adenocarcinoma that cause Cancer.
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