Markers of Polyfunctional SARS-Cov-2 Antibodies in Convalescent Plasma
RESEARCH ARTICLE Markers of Polyfunctional SARS-CoV-2 Antibodies in Convalescent Plasma Harini Natarajan,a Andrew R. Crowley,a Savannah E. Butler,a Shiwei Xu,b Joshua A. Weiner,b Evan M. Bloch,c Kirsten Littlefield,d Wendy Wieland-Alter,e Ruth I. Connor,e Peter F. Wright,e Sarah E. Benner,c Tania S. Bonny,c Oliver Laeyendecker,f,g David Sullivan,d,f Shmuel Shoham,f Thomas C. Quinn,f,g H. Benjamin Larman,c Arturo Casadevall,d,f Andrew Pekosz,d Andrew D. Redd,f,g Aaron A. R. Tobian,c Margaret E. Ackermana,b aDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Dartmouth College, Hanover, New Hampshire, USA bThayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA cDepartment of Pathology, Johns Hopkins School of Medicine, Baltimore, Maryland, USA dW. Harry Feinstone Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA eDepartment of Pediatrics, Geisel School of Medicine at Dartmouth, Dartmouth-Hitchcock Medical Center, Lebanon, New Hampshire, USA fDepartment of Medicine, Division of Infectious Diseases, Johns Hopkins School of Medicine, Baltimore, Maryland, USA gDivision of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA Harini Natarajan, Andrew R. Crowley, and Savannah E. Butler contributed equally. Authors are arranged in reverse alphabetical order. Aaron A. R. Tobian and Margaret E. Ackerman are Co-senior authors. ABSTRACT Convalescent plasma is a promising therapy for coronavirus disease 2019 (COVID-19), but the antibody characteristics that contribute to efficacy remain poorly understood. This study analyzed plasma samples from 126 eligible convales- cent blood donors in addition to 15 naive individuals, as well as an additional 20 convalescent individuals as a validation cohort.
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