Polyclonal Antibody to PDE4D - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP06558PU-N Polyclonal Antibody to PDE4D - Aff - Purified

Alternate names: 5'-cyclic phosphodiesterase 4D, DPDE3, DPDE3, EC=3.1.4.17, PDE43, PDE4D, cAMP- specific 3' Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: Phosphodiesterases (PDE) hydrolyze cAMP to 5’AMP and thus play a critical role in the regulation of intracellular cAMP. Division of the PDE superfamily by sequence homology and enzymatic properties yields 11 PDE families. A unique upstream conserved region (UCR) characterizes the PDE4 family. Four separate genes (A-D) encode the PDE4 enzymes, and alternative splicing generates short or long isoforms of each gene. Long PDE4 isoforms contain both UCR1 and UCR2 while short PDE4 isoforms possess only UCR2. Both UCR domains are necessary for dimerization of PDE4 isoforms. The human PDE4D gene maps to chromosome 5q12. The splice variants include isoforms PDE4D1-6. Uniprot ID: Q08499 NCBI: NP_001098101.1 GeneID: 5144 Host: Rabbit Immunogen: Synthetic peptide, corresponding to amino acids 30-70 Human PDE4D. Format: State: Liquid purified Ig fraction Purification: Affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) Buffer System: Phosphate buffered saline (PBS), pH 7.2. Preservatives: 15 mM sodium azide Applications: Western blot: 1/500-1/1000. Immunohistochemistry on paraffin sections: 1/50-1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Molecular Weight: ~ 88 kDa Specificity: Thhis antibody detects endogenous levels of PDE4D protein. (region surrounding His47) Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse and Rat. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. VA/20140905 1 / 2 AP06558PU-N: Polyclonal Antibody to PDE4D - Aff - Purified

Pictures: Immunohistochemistry analysis of PDE4D antibody (Cat.-No.: AP06558PU-N) in paraffin-embedded human colon carcinoma tissue.

Western blot analysis of PDE4D antibody (Cat.-No.: AP06558PU-N) in extracts from COS7 cells treated with Nocodazole 1 ug/ml 16 h.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. VA/20140905 2 / 2