bioRxiv preprint doi: https://doi.org/10.1101/262600; this version posted February 9, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. 1 Complex evolutionary history of translation Elongation Factor 2 and diphthamide 2 biosynthesis in Archaea and parabasalids 3 4 Adrienne B. Narrowe1,†, Anja Spang2,3,†, Courtney W. Stairs2, Eva F. Caceres2, Brett J. Baker4, 5 Christopher S. Miller1,‡,* and Thijs J. G. Ettema2,‡ 6 7 † These authors contributed equally to this work. 8 ‡These authors contributed equally to this work. 9 *Author for correspondence: Christopher S. Miller, Department of Integrative Biology, 10 University of Colorado Denver, Denver, CO, USA, 303-315-7665,
[email protected] 11 12 1 Department of Integrative Biology, University of Colorado Denver, Denver, Colorado, USA 13 2 Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, 14 Uppsala, Sweden 15 3 NIOZ, Royal Netherlands Institute for Sea Research, Department of Marine Microbiology and 16 Biogeochemistry, and Utrecht University, AB Den Burg, The Netherlands 17 4 Department of Marine Science, Marine Science Institute, University of Texas Austin, Port 18 Aransas, TX, USA 19 20 21 Running title: EF-2 and diphthamide in archaea and eukaryotes 22 1 bioRxiv preprint doi: https://doi.org/10.1101/262600; this version posted February 9, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.