Point Spread Functions in Identification of Astronomical Objects

Total Page:16

File Type:pdf, Size:1020Kb

Point Spread Functions in Identification of Astronomical Objects RADIOENGINEERING, VOL. 25, NO. 1, APRIL 2016 169 Point Spread Functions in Identification of Astronomical Objects from Poisson Noised Image František MOJŽÍŠ1, Jaromír KUKAL1, Jan ŠVIHLÍK1;2 1Dept. of Computing and Control Eng., University of Chemistry and Technology Prague, Technická 5, 166 28 Prague, Czech Republic 2Center for Machine Perception, Department of Cybernetics, Czech Technical University in Prague, Technická 2, 166 28 Prague, Czech Republic [email protected], jaromir.kukal@fjfi.cvut.cz, [email protected] Manuscript received August 5, 2015 Abstract. This article deals with modeling of astronomi- of this system, also called Point Spread Function (PSF). PSF cal objects, which is one of the most fundamental topics in of applied imaging system is influenced by many factors astronomical science. Introduction part is focused on prob- and is composed as a convolution of particular PSFs of sys- lem description and used methods. Point Spread Function tem’s components. The knowledge or a good estimate of the Modeling part deals with description of basic models used resulting system’s PSF plays a key role in astronomical pho- in astronomical photometry and further on introduction of tometry [3, 4], when we want to know accurate information more sophisticated models such as combinations of interfer- about the observed objects. ence, turbulence, focusing, etc. This paper also contains a way of objective function definition based on the knowledge of Poisson distributed noise, which is included in astronom- ical data. The proposed methods are further applied to real astronomical data. Keywords Astronomical image, point spread function, Poisson noise, objective function 1. Introduction Astronomy is a natural science that deals with the study of objects such as planets, moons, stars, etc. Modeling of the mentioned objects is one of the most fundamental topics Fig. 1. Analyzed astronomical image. in astronomical science and the branch dealing with object identification is called astronomical photometry. Under the Astronomical images can be expressed in mathematical term modeling can we understand finding of exact infor- way as follows mation about objects parameters such like its coordinates, x(k; l) = f(k; l) + n(k; l) (1) magnitude, etc. Previously mentioned objects are far away from the where f(k; l) are the data and n(k; l) represents noise called Earth and thus they appear as a bright point on the night sky. the dark current. This type of noise is caused by thermally When an astronomical image, Fig. 1, is acquired, the situa- generated charge, due to the long exposure times. Dark cur- tion is quite different and the bright point has changed and rent can be simply removed by a dark frame, which maps as a result the image will appear as smeared pattern. This is mentioned thermally generated charge in CCD sensor. It caused by passing of original information through the imag- can be considered that this type of noise is Poisson dis- ing system [1, 2] used for an image acquisition. The result tributed [5, 6, 7] in the following way shape of captured objects is given by the impulse response n(k; l) ∼ Poisson(λ(k; l)): (2) DOI: 10.13164/re.2016.0169 SIGNALS 170 F. MOJŽÍŠ, J. KUKAL, J. ŠVIHLÍK, POINT SPREAD FUNCTIONS IN IDENTIFICATION OF ASTRONOMICAL OBJECTS . This article is intended to present commonly used PSF do not comprise some important facts. If we consider that models such as Gauss and Moffat which are popular in PSF the light passes through the optical system before incidence modelling are easy to evaluate and provide good approxi- onto the image sensor, than it is possible to make an approx- mation of analyzed objects [7, 8, 9, 10]. Another physical imation of the result system PSF by diffraction of circular phenomena known as interference, focusing and atmospheric aperture [17] turbulence are rarely used [8, 11, 12, 13]. The main aim of 2J (ka sin θ) I(θ) = I · 1 (6) this article is to compare commonly used methods with PSF’s 0 ka sin θ based on their combinations to show their advantage in PSF I modelling. where 0 is the maximum intensity of the pattern, J1 is Bessel function of the first order, k = 2π=λ, λ is the wavenumber, Optimization of described PSF models is based on au- a is the radius of the aperture and θ is the angle of observa- thors previous work dealing with detection [5] and identi- tion. fication [14] of analyzed objects based on analysis of dark and light frames using hypotheses testing. One of the key From physics, it is known that the diffraction phe- roles plays also the way of the objective function estima- nomenon described by (6) is accompanied by the interference tion. Objective functions determination based on hypothesis phenomenon [17] and thus we can call this relation as inter- of Poisson noise is described in Section 3 as well as brief ference model, which is in the frequency domain expressed as ( introduction of Harmony Search algorithm which was used 1 if ! ≤ Ω; G(!) = (7) for purpose of objective function optimization. 0 otherwise where Ω > 0 is frequency and therefore adequate PSF is Z1 2J (Ωr) 2. Point Spread Function Modeling g(r) = H fG(!)g = 2π !G(!)J (!r)d! = 1 0 Ωr Astronomical photometry based on the two- 0 dimensional fitting uses the hypothesis that the profiles (8) where H is Hankel transform [18]. of astronomical point sources which are imaged on two- dimensional arrays are commonly referred to as PSF [2, 15], Another important factor that influences resulting image is passing of the information through the atmospheric condi- x(x; y) = object(x; y) ∗ PSF(x; y) (3) tions. Thus, important phenomenon that influences result im- age is atmospheric turbulence. The interference phenomenon where ∗ is a convolution operator and x, object, PSF are occurs in any optical system with constrained view. The PSF 2D functions, which represent the result image, the original of interference is the well known Airy disc [19]. The main object and the system response, respectively. PSFs can be question is whether the interference dominates the other pro- modeled by a number of simple or more complex mathe- cesses, only affects them or is dominated by them. According matical functions that are derived from deeper knowledge of to [19] we can express frequency spectrum of the turbulence studied problem. as 2 1 S(!) = σ2 (9) 2.1 Basic PSF Models π 1 + ( !)2 where is the scale of the turbulence and σ > 0 represents Statistical models based on different PSFs are com- the standard deviation of the velocity disturbance. monly used for objects localization in astronomical science. There are usually applied two simple models, i.e., the first The role of atmospheric turbulence increases mainly in one is two-dimensional Gaussian function [3] the case of wrong weather and observation conditions. 2 2 ! (k − x0) + (l − y0) The last phenomena described in this article and that fG(k; l; p) = A · exp − (4) 2σ2 can influence result PSF is focusing [20, 21], which can be expressed as follows where A is amplitude, x0, y0 are shifts in the x − y plane, 1 σ > 0 is its standard deviation and k, l are pixel indices as (r) = · δ(r; ρ) f 2 (10) coordinates. πρ The second model is statistical model described by Mof- where ρ > 0 is focusing radius and fat [3, 16], which is a generalization of Cauchy distribution ( 1 if r ≤ ρ; δ(r; ρ) = (11) A 0 otherwise; fM(k; l; p) = (5) 2 2 β (k−x0) +(l−y0) 1 + 2 σ Z 1 2J1(!ρ) where β is a shape parameter of PDF satisfying 0 ≤ β ≤ 50. F(!) = H ff(r)g = 2π rf(r)J0(!r)dr = : 0 !ρ As mentioned above, these two presented models are (12) commonly used in astronomical photometry using (3), but RADIOENGINEERING, VOL. 25, NO. 1, APRIL 2016 171 The focusing can be easily eliminated by setting ρ ! 0+ in the case of perfect focusing of real telescope. H(!) = FG(!) · F(!) · G(!); (19) But the importance of non-perfect focusing is in its combina- tion with interference in real telescope as will be explained and finally combination of Interference, Focusing and Tur- in the next section. bulence (IFT) 2.2 Combined Models H(!) = S(!) · F(!) · G(!): (20) In this article, there are compared previously mentioned This approach was used due to the reason that ana- models, i.e., Gauss, Moffat, Interference, Turbulence and Fo- lytical convolution of S(!) to s(r) is impossible, thus the cusing with more sophisticated models given by their con- data analyzed by combined models are processed only in the volutions, excluding Moffat. Thus, we can find 7 possible frequency domain, subsequently transformed into spatial do- combinations we can use. Possible combinations are listed main and properly modified for amplitude A, x0 and y0 shifts below and always mentioned in frequency domain with re- optimization. spect to the convolution theorem, as multiplying of Fourier Parameters of particular single models and their lower images. and upper bounds can be found it Tabs. 1 and 2, respectively. Those are Interference in combination with Turbulence (IT) PSF model parameters Gauss σ Moffat σ, β H(!) = G(!) · S(!) (13) Interference Ω Focusing ρ Turbulence , σ which is model of constrained observation in the case of Tab. 1. Parameters of basic PSFs. wrong atmospheric condition. Fortunately, the effect of tur- bulence can eliminate Airy disc around every point light model bound p1 p2 p3 p4 p5 p6 LB 1 1 1 1 source. Gauss UB 2 max(x(k; l) − d(k; l)) M N max(M; N) LB 1 1 1 1 0.01 Interference plus Focusing (IF) Moffat UB 2 max(x(k; l) − d(k; l)) M N max(M; N) 50 LB 1 1 1 0.01 0.0001 0.0001 Interference H(!) = G(!) · F(!) (14) UB 2 max(x(k; l) − d(k; l)) M N 10 10 10 LB 1 1 1 0.01 0.01 Focusing which is model of constrained observation in the case UB 2 max(x(k; l) − d(k; l)) M N 10 10 LB 1 1 1 0.01 0.01 of wrong focusing.
Recommended publications
  • Single‑Molecule‑Based Super‑Resolution Imaging
    Histochem Cell Biol (2014) 141:577–585 DOI 10.1007/s00418-014-1186-1 REVIEW The changing point‑spread function: single‑molecule‑based super‑resolution imaging Mathew H. Horrocks · Matthieu Palayret · David Klenerman · Steven F. Lee Accepted: 20 January 2014 / Published online: 11 February 2014 © Springer-Verlag Berlin Heidelberg 2014 Abstract Over the past decade, many techniques for limit, gaining over two orders of magnitude in precision imaging systems at a resolution greater than the diffraction (Szymborska et al. 2013), allowing direct observation of limit have been developed. These methods have allowed processes at spatial scales much more compatible with systems previously inaccessible to fluorescence micros- the regime that biomolecular interactions take place on. copy to be studied and biological problems to be solved in Radically, different approaches have so far been proposed, the condensed phase. This brief review explains the basic including limiting the illumination of the sample to regions principles of super-resolution imaging in both two and smaller than the diffraction limit (targeted switching and three dimensions, summarizes recent developments, and readout) or stochastically separating single fluorophores in gives examples of how these techniques have been used to time to gain resolution in space (stochastic switching and study complex biological systems. readout). The latter also described as follows: Single-mol- ecule active control microscopy (SMACM), or single-mol- Keywords Single-molecule microscopy · Super- ecule localization microscopy (SMLM), allows imaging of resolution imaging · PALM/(d)STORM imaging · single molecules which cannot only be precisely localized, Localization microscopy but also followed through time and quantified. This brief review will focus on this “pointillism-based” SR imaging and its application to biological imaging in both two and Fluorescence microscopy allows users to dynamically three dimensions.
    [Show full text]
  • Mapping the PSF Across Adaptive Optics Images
    Mapping the PSF across Adaptive Optics images Laura Schreiber Osservatorio Astronomico di Bologna Email: [email protected] Abstract • Adaptive Optics (AO) has become a key technology for all the main existing telescopes (VLT, Keck, Gemini, Subaru, LBT..) and is considered a kind of enabling technology for future giant telescopes (E-ELT, TMT, GMT). • AO increases the energy concentration of the Point Spread Function (PSF) almost reaching the resolution imposed by the diffraction limit, but the PSF itself is characterized by complex shape, no longer easily representable with an analytical model, and by sometimes significant spatial variation across the image, depending on the AO flavour and configuration. • The aim of this lesson is to describe the AO PSF characteristics and variation in order to provide (together with some AO tips) basic elements that could be useful for AO images data reduction. Erice School 2015: Science and Technology with E-ELT What’s PSF • ‘The Point Spread Function (PSF) describes the response of an imaging system to a point source’ • Circular aperture of diameter D at a wavelenght λ (no aberrations) Airy diffraction disk 2 퐼휃 = 퐼0 퐽1(푥)/푥 Where 퐽1(푥) represents the Bessel function of order 1 푥 = 휋 퐷 휆 푠푛휗 휗 is the angular radius from the aperture center First goes to 0 when 휗 ~ 1.22 휆 퐷 Erice School 2015: Science and Technology with E-ELT Imaging of a point source through a general aperture Consider a plane wave propagating in the z direction and illuminating an aperture. The element ds = dudv becomes the a sourse of a secondary spherical wave.
    [Show full text]
  • Topic 3: Operation of Simple Lens
    V N I E R U S E I T H Y Modern Optics T O H F G E R D I N B U Topic 3: Operation of Simple Lens Aim: Covers imaging of simple lens using Fresnel Diffraction, resolu- tion limits and basics of aberrations theory. Contents: 1. Phase and Pupil Functions of a lens 2. Image of Axial Point 3. Example of Round Lens 4. Diffraction limit of lens 5. Defocus 6. The Strehl Limit 7. Other Aberrations PTIC D O S G IE R L O P U P P A D E S C P I A S Properties of a Lens -1- Autumn Term R Y TM H ENT of P V N I E R U S E I T H Y Modern Optics T O H F G E R D I N B U Ray Model Simple Ray Optics gives f Image Object u v Imaging properties of 1 1 1 + = u v f The focal length is given by 1 1 1 = (n − 1) + f R1 R2 For Infinite object Phase Shift Ray Optics gives Delta Fn f Lens introduces a path length difference, or PHASE SHIFT. PTIC D O S G IE R L O P U P P A D E S C P I A S Properties of a Lens -2- Autumn Term R Y TM H ENT of P V N I E R U S E I T H Y Modern Optics T O H F G E R D I N B U Phase Function of a Lens δ1 δ2 h R2 R1 n P0 P ∆ 1 With NO lens, Phase Shift between , P0 ! P1 is 2p F = kD where k = l with lens in place, at distance h from optical, F = k0d1 + d2 +n(D − d1 − d2)1 Air Glass @ A which can be arranged to|giv{ze } | {z } F = knD − k(n − 1)(d1 + d2) where d1 and d2 depend on h, the ray height.
    [Show full text]
  • Diffraction Optics
    ECE 425 CLASS NOTES – 2000 DIFFRACTION OPTICS Physical basis • Considers the wave nature of light, unlike geometrical optics • Optical system apertures limit the extent of the wavefronts • Even a “perfect” system, from a geometrical optics viewpoint, will not form a point image of a point source • Such a system is called diffraction-limited Diffraction as a linear system • Without proof here, we state that the impulse response of diffraction is the Fourier transform (squared) of the exit pupil of the optical system (see Gaskill for derivation) 207 DR. ROBERT A. SCHOWENGERDT [email protected] 520 621-2706 (voice), 520 621-8076 (fax) ECE 425 CLASS NOTES – 2000 • impulse response called the Point Spread Function (PSF) • image of a point source • The transfer function of diffraction is the Fourier transform of the PSF • called the Optical Transfer Function (OTF) Diffraction-Limited PSF • Incoherent light, circular aperture ()2 J1 r' PSF() r' = 2-------------- r' where J1 is the Bessel function of the first kind and the normalized radius r’ is given by, 208 DR. ROBERT A. SCHOWENGERDT [email protected] 520 621-2706 (voice), 520 621-8076 (fax) ECE 425 CLASS NOTES – 2000 D = aperture diameter πD πr f = focal length r' ==------- r -------- where λf λN N = f-number λ = wavelength of light λf • The first zero occurs at r = 1.22 ------ = 1.22λN , or r'= 1.22π D Compare to the course notes on 2-D Fourier transforms 209 DR. ROBERT A. SCHOWENGERDT [email protected] 520 621-2706 (voice), 520 621-8076 (fax) ECE 425 CLASS NOTES – 2000 2-D view (contrast-enhanced) “Airy pattern” central bright region, to first- zero ring, is called the “Airy disk” 1 0.8 0.6 PSF 0.4 radial profile 0.2 0 0246810 normalized radius r' 210 DR.
    [Show full text]
  • Improved Single-Molecule Localization Precision in Astigmatism-Based 3D Superresolution
    bioRxiv preprint doi: https://doi.org/10.1101/304816; this version posted April 19, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Improved single-molecule localization precision in astigmatism-based 3D superresolution imaging using weighted likelihood estimation Christopher H. Bohrer1Ϯ, Xinxing Yang1Ϯ, Zhixin Lyu1, Shih-Chin Wang1, Jie Xiao1* 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland, 21205, USA. Ϯ These authors contributed equally to this work. *Correspondence and requests for materials should be addressed J.X. (email: [email protected]). Abstract: Astigmatism-based superresolution microscopy is widely used to determine the position of individual fluorescent emitters in three-dimensions (3D) with subdiffraction-limited resolutions. This point spread function (PSF) engineering technique utilizes a cylindrical lens to modify the shape of the PSF and break its symmetry above and below the focal plane. The resulting asymmetric PSFs at different z-positions for single emitters are fit with an elliptical 2D-Gaussian function to extract the widths along two principle x- and y-axes, which are then compared with a pre-measured calibration function to determine its z-position. While conceptually simple and easy to implement, in practice, distorted PSFs due to an imperfect optical system often compromise the localization precision; and it is laborious to optimize a multi-purpose optical system. Here we present a methodology that is independent of obtaining a perfect PSF and enhances the localization precision along the z-axis.
    [Show full text]
  • List of Abbreviations STEM Scanning Transmission Electron Microscopy
    List of Abbreviations STEM Scanning Transmission Electron Microscopy PSF Point Spread Function HAADF High-Angle Annular Dark-Field Z-Contrast Atomic Number-Contrast ICM Iterative Constrained Methods RL Richardson-Lucy BD Blind Deconvolution ML Maximum Likelihood MBD Multichannel Blind Deconvolution SA Simulated Annealing SEM Scanning Electron Microscopy TEM Transmission Electron Microscopy MI Mutual Information PRF Point Response Function Registration and 3D Deconvolution of STEM Data M. MERCIMEK*, A. F. KOSCHAN*, A. Y. BORISEVICH‡, A.R. LUPINI‡, M. A. ABIDI*, & S. J. PENNYCOOK‡. *IRIS Laboratory, Electrical Engineering and Computer Science, The University of Tennessee, 1508 Middle Dr, Knoxville, TN 37996 ‡Materials Science and Technology Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831 Key words. 3D deconvolution, Depth sectioning, Electron microscope, 3D Reconstruction. Summary A common characteristic of many practical modalities is that imaging process distorts the signals or scene of interest. The principal data is veiled by noise and less important signals. We split the 3D enhancement process into two separate stages; deconvolution in general is referring to the improvement of fidelity in electronic signals through reversing the convolution process, and registration aims to overcome significant misalignment of the same structures in lateral planes along focal axis. The dataset acquired with VG HB603U STEM operated at 300 kV, producing a beam with a diameter of 0.6 A˚ equipped with a Nion aberration corrector has been studied. The initial experiments described how aberration corrected Z- Contrast Imaging in STEM can be combined with the enhancement process towards better visualization with a depth resolution of a few nanometers. I. INTRODUCTION 3D characterization of both biological samples and non-biological materials provides us nano-scale resolution in three dimensions permitting the study of complex relationships between structure and existing functions.
    [Show full text]
  • Confocal Microscopes
    Faculty of Science Debye Institute Measuring point spread functions and calibrating (STED) confocal microscopes Bachelor thesis Peter N.A. Speets Physics and Astronomy main supervisors Prof.dr. Alfons van Blaaderen Soft Condensed Matter Group Dr. Arnout Imhof Soft Condensed Matter Group daily supervisor Ernest B. van der Wee Soft Condensed Matter Group June 2016 Abstract In this thesis the point spread function (PSF) used to characterize a confocal microscope was determined for different configurations. The PSF was determined for a sample with a refractive index matched to the refractive index that the objective was designed for, which is in this case n = 1:518 and n = 1:451 [1], and it was investigated how a mismatch in refractive index affects the shape and size of the PSF. Also the PSF of stimulated emission depletion (STED) confocal microscopy was determined to investigate the advantages of STED over conventional confocal microscopy. The alignment of the STED and the excitation laser of the confocal microscope was checked by imaging the reflection of the STED and excitation laser with a gold core nanoparticle. For the calibration of the distance measurement in a sample with a mismatch in refractive index a colloidal crystal was grown to compare the interlayer distance determined by using FTIR spectroscopy and the interlayer distance determined by imaging the crystal with the confocal microscope. 2 1. INTRODUCTION 3 1 Introduction The purpose of confocal microscopy, compared to brightfield microscopy, is to increase resolution and contrast. The confocal microscope ensures that only light from the focus point in the sample is imaged.
    [Show full text]
  • Assessing Visual Quality with the Point Spread Function Using the NIDEK OPD-Scan II
    Assessing Visual Quality With the Point Spread Function Using the NIDEK OPD-Scan II Edoardo A. Ligabue, MD; Cristina Giordano, OD he measurement of high-contrast visual acuity is ABSTRACT not an adequate representation of the patient’s vi- PURPOSE: To present the use of the point spread sual quality. Visual acuity can be described by the T 1 function (PSF) metric pre- and postoperatively for the following expression : V • A • = 1/␣, where ␣ represents the assessment of visual quality in cataract and refractive minimum angle of resolution. Snellen acuity is a measure of the surgery. ability of the eye to resolve details, and the recorded value pro- vides a metric of the functional integrity of the optical system. METHODS: Case examples of cataract and refractive However, vision is a complex neural process, and the percep- surgery and the effect of accommodation are presented. All PSF measurements were obtained using the NIDEK tion of scotopic symptoms such as halos and glare cannot be OPD-Scan II, and corneal internal and total aberrations described by a single value metric. were simulated using the NIDEK OPD-Station software. Theoretically, a perfect optical system would focus all in- All eyes underwent corneal topography, wavefront aber- coming light rays into one single point that would not cause rometry, autorefraction, keratometry, and pupillometry halos or glare. In the human eye, however, light is distorted, measurements pre- and postoperatively using the OPD- Scan II. The PSF was used to assess visual quality. and this distortion can be measured using wavefront aber- rometry.2 The wave theory of light can be used to explain RESULTS: Four case examples including refractive sur- interference and the diffraction primarily responsible for gery, aspheric multifocal intraocular lens (IOL) implanta- less-than-perfect optics in the human eye that form the basis tion, toric IOL implantation, and the difference in PSF of the point spread function (PSF).3 For example, when the due to accommodation are presented.
    [Show full text]
  • An Analytical Approximation of the Hubble Space Telescope Monochromatic Point Spread Functions
    J. Astrophys. Astr. (1987) 8, 343–350 An Analytical Approximation of the Hubble Space Telescope Monochromatic Point Spread Functions Ο. Bendinelli Dipartimento di Astronomia, Università di Bologna, Via Zamboni 33, 40126 Bologna, Italy G. Parmeggiani Osservatorio Astronomico di Bologna, Via Zamboni 33, 40126 Bologna, Italy F. Zavatti Dipartimento di Astronomia, Università di Bologna, Via Zamboni 33, 40126 Bologna, Italy Received 1987 June 16; accepted 1987 October 7 Abstract. A mixture of off-centred Gaussians is used to approximate the predicted monochromatic point spread functions (PSFs) of Hubble space telescope (HST) at long wavelengths, and also, with a reduced number of parameters, the wide-band PSFs. The usefulness of the approximation in simulation and analysis of HST data is shown by means of application to image centring and convolution-deconvolution problems. Key words: HST, point spread function—image centring—image decon- volution 1, Introduction The basic information on the expected image quality of the Hubble space telescope (HST) is reported in the last Announcement of Opportunity (NASA 1984, A.O.No.OSSA-4-84, hereinafter AO). The resulting wavelength-dependent point spread functions (PSFs) are characterized (see Figs 2-E to 14-Ε in AO) by an irregular oscillatory behaviour due to superposition of the diffraction patterns of primary and secondary mirrors, a total wavefront error of 0.05 wavelength RMS (at 633 nm), and an estimated pointing instability which causes image motion with 0.007 arcsec rms. As noted by Bendinelli et al. (1985), the oscillatory behaviour at longer wavelengths does not disappear after PSF integration over pixel surface and bandwidth, though it evidently becomes smoother.
    [Show full text]
  • Psfs and Sampling of Images in Astronomy 8/25/10 4:24 PM
    PSFs and Sampling of Images in Astronomy 8/25/10 4:24 PM UP | HOME PSFs and Sampling of Images in Astronomy Already first section. (press any key to proceed) Up / HOME / HELP / toggle view Table of Contents 1 Moffat PSFs 2 The PSF as derived from models of atmospheric turbulence 3 Gaussian and multi-Gaussian PSFs 4 Airy Patterns and Diffraction Limited PSFs 5 Nyquist sampling and a band limited images 1 Moffat PSFs Moffat proposed using a softened exponential profile to model a PSF: Note that β determines the overall shape of the PSF, while α is a scale factor. The FWHM is twice the value of r at which I(r) = 0.5 I0, so derivation of the value of α corresponding to a given FWHM is straightforward. moffat <- function(r,fwhm,beta=4.765) { hwhm <- (fwhm/2.0) alpha <- hwhm*(2^(1.0/beta)-1)^(-0.5) p <- (1.0 + (r/alpha)^2.0)^(-1.0*beta) p/sum(p) } http://home.fnal.gov/~neilsen/notebook/astroPSF/astroPSF.html#undefined Page 1 of 4 PSFs and Sampling of Images in Astronomy 8/25/10 4:24 PM 2 The PSF as derived from models of atmospheric turbulence Fried (J. Opt. Soc. Am., Volume 56, p. 1372-1379) (as reported by Trujillo etal) and Woolf (1982) use atmospheric turbulence theory to show that the PSF is the Fourier transform of an exponential: where Trujillo etal find that this closely matches a Moffat PSF with β = 4.765. Typical values of β measured from astronomical images have lower values of β (Saglia etal (1993MNRAS.264..961S)), but not as low as the default value of 2.5 used by some IRAF routines.
    [Show full text]
  • Single-Molecule Localization Microscopy Hardware
    PRIMER Single-molecule localization microscopy Mickaël Lelek1,2, Melina T. Gyparaki3, Gerti Beliu4, Florian Schueder 5,6, Juliette Griffié7, Suliana Manley 7 ✉ , Ralf Jungmann 5,6 ✉ , Markus Sauer 4 ✉ , Melike Lakadamyali8,9,10 ✉ and Christophe Zimmer 1,2 ✉ Abstract | Single- molecule localization microscopy (SMLM) describes a family of powerful imaging techniques that dramatically improve spatial resolution over standard, diffraction-limited microscopy techniques and can image biological structures at the molecular scale. In SMLM, individual fluorescent molecules are computationally localized from diffraction- limited image sequences and the localizations are used to generate a super- resolution image or a time course of super- resolution images, or to define molecular trajectories. In this Primer, we introduce the basic principles of SMLM techniques before describing the main experimental considerations when performing SMLM, including fluorescent labelling, sample preparation, hardware requirements and image acquisition in fixed and live cells. We then explain how low-resolution image sequences are computationally processed to reconstruct super-resolution images and/or extract quantitative information, and highlight a selection of biological discoveries enabled by SMLM and closely related methods. We discuss some of the main limitations and potential artefacts of SMLM, as well as ways to alleviate them. Finally, we present an outlook on advanced techniques and promising new developments in the fast-evolving field of SMLM. We hope that this Primer will be a useful reference for both newcomers and practitioners of SMLM. Diffraction The spatial resolution of standard optical microscopy They have become broadly adopted in the life sciences The bending of light waves at techniques is limited to roughly half the wavelength of owing to their high spatial resolution — typically the edges of an obstacle such light.
    [Show full text]
  • Fast Point Spread Function Modeling with Deep Learning
    Prepared for submission to JCAP Fast Point Spread Function Modeling with Deep Learning Jörg Herbel,a Tomasz Kacprzak,a Adam Amara,a Alexandre Refregier,a Aurelien Lucchib aInstitute for Particle Physics and Astrophysics, Department of Physics, ETH Zurich, Wolfgang- Pauli-Strasse 27, CH-8093 Zurich, Switzerland bData Analytics Lab, Department of Computer Science, ETH Zurich, Universitätstrasse 6, CH-8092 Zurich, Switzerland E-mail: [email protected], [email protected], [email protected], [email protected], [email protected] Abstract. Modeling the Point Spread Function (PSF) of wide-field surveys is vital for many astrophysical applications and cosmological probes including weak gravitational lensing. The PSF smears the image of any recorded object and therefore needs to be taken into account when inferring properties of galaxies from astronomical images. In the case of cosmic shear, the PSF is one of the dominant sources of systematic errors and must be treated carefully to avoid biases in cosmological parameters. Recently, forward modeling approaches to calibrate shear measurements within the Monte-Carlo Control Loops (MCCL) framework have been developed. These methods typically require simulating a large amount of wide-field images, thus, the simulations need to be very fast yet have realistic properties in key features such as the PSF pattern. Hence, such forward modeling approaches require a very flexible PSF model, which is quick to evaluate and whose parameters can be estimated reliably from survey data. We present a PSF model that meets these requirements based on a fast deep-learning method to estimate its free parameters.
    [Show full text]