REEP5 Polyclonal Antibody

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PRODUCT DATA SHEET Bioworld Technology,Inc. REEP5 polyclonal antibody Catalog: BS71516 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: The antibody was affinity-purified from rabbit antiserum REEP5 (receptor expression-enhancing protein 5), also by affinity-chromatography using epitope-specific im- known as C5orf18, DP1, TB2 or D5S346, is a 189 amino munogen and the purity is > 95% (by SDS-PAGE). acid multi-pass membrane protein. Thought to promote Applications: the functional cell surface expression of olfactory recep- WB 1:500 - 1:2000 tors, REEP5 belongs to the DP1 family and is encoded by Storage&Stability: a gene that maps to chromosome 5. With 181 million base Store at 4°C short term. Aliquot and store at -20°C long pairs encoding around 1,000 genes, chromosome 5 is term. Avoid freeze-thaw cycles. about 6% of human genomic DNA. Chromosome 5 is Specificity: associated with Cockayne syndrome through the ERCC8 REEP5 polyclonal antibody detects endogenous levels of gene and familial adenomatous polyposis through the REEP5 protein. adenomatous polyposis coli (APC) tumor suppressor DATA: gene. Treacher Collins syndrome is also chromosome 5 associated and is caused by insertions or deletions within the TCOF1 gene. Deletion of the parm of chromosome 5 leads to cri du chat syndrome. Deletion of 5q or chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, Western blot analysis of extracts of various cell lines, using REEP5 an- 50% glycerol, pH7.2 tibody. Molecular Weight: Note: ~ 21 kDa For research use only, not for use in diagnostic procedure. Swiss-Prot: Q00765 Purification&Purity: Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .

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Ck1δ Over-Expressing Mice Display ADHD-Like Behaviors, Frontostriatal Neuronal Abnormalities and Altered Expressions of ADHD-Candidate Genes
Molecular Psychiatry (2020) 25:3322–3336 https://doi.org/10.1038/s41380-018-0233-z ARTICLE CK1δ over-expressing mice display ADHD-like behaviors, frontostriatal neuronal abnormalities and altered expressions of ADHD-candidate genes 1 1 1 2 1 1 1 Mingming Zhou ● Jodi Gresack ● Jia Cheng ● Kunihiro Uryu ● Lars Brichta ● Paul Greengard ● Marc Flajolet Received: 8 November 2017 / Revised: 4 July 2018 / Accepted: 18 July 2018 / Published online: 19 October 2018 © Springer Nature Limited 2018 Abstract The cognitive mechanisms underlying attention-deficit hyperactivity disorder (ADHD), a highly heritable disorder with an array of candidate genes and unclear genetic architecture, remain poorly understood. We previously demonstrated that mice overexpressing CK1δ (CK1δ OE) in the forebrain show hyperactivity and ADHD-like pharmacological responses to D- amphetamine. Here, we demonstrate that CK1δ OE mice exhibit impaired visual attention and a lack of D-amphetamine- induced place preference, indicating a disruption of the dopamine-dependent reward pathway. We also demonstrate the presence of abnormalities in the frontostriatal circuitry, differences in synaptic ultra-structures by electron microscopy, as 1234567890();,: 1234567890();,: well as electrophysiological perturbations of both glutamatergic and GABAergic transmission, as observed by altered frequency and amplitude of mEPSCs and mIPSCs. Furthermore, gene expression profiling by next-generation sequencing alone, or in combination with bacTRAP technology to study specifically Drd1a versus Drd2 medium spiny neurons, revealed that developmental CK1δ OE alters transcriptional homeostasis in the striatum, including specific alterations in Drd1a versus Drd2 neurons. These results led us to perform a fine molecular characterization of targeted gene networks and pathway analysis. Importantly, a large fraction of 92 genes identified by GWAS studies as associated with ADHD in humans are significantly altered in our mouse model.
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SAN TA C RUZ BI OTEC HNOL OG Y, INC . REEP5 (E-13): sc-133405 BACKGROUND RECOMMENDED SECONDARY REAGENTS REEP5 (receptor expression-enhancing protein 5), also known as C5orf18, To ensure optimal results, the following support (secondary) reagents are DP1, TB2 or D5S346, is a 189 amino acid multi-pass membrane protein. recommended: 1) Western Blotting: use goat anti-rabbit IgG-HRP: sc-2004 Thought to promote the functional cell surface expression of olfactory recep - (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible goat anti- tors, REEP5 belongs to the DP1 family and is encoded by a gene that maps rabbit IgG-HRP: sc-2030 (dilution range: 1:2000-1:5000), Cruz Marker™ to chromosome 5. With 181 million base pairs encoding around 1,000 genes, Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: chromosome 5 is about 6% of human genomic DNA. Chromosome 5 is sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunoprecip- asso ciated with Cockayne syndrome through the ERCC8 gene and familial itation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). adenomatous polyposis through the adenomatous polyposis coli (APC) tumor 3) Immunofluorescence: use goat anti-rabbit IgG-FITC: sc-2012 (dilution suppressor gene. Treacher Collins syndrome is also chromosome 5 associat ed range: 1:100-1:400) or goat anti-rabbit IgG-TR: sc-2780 (dilution range: and is caused by insertions or deletions within the TCOF1 gene. Deletion of 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. the p arm of chromosome 5 leads to Cri-du-chat syndrome.
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