Assessment of the Drug-Drug Interaction Potential of Anionic Components in the Diet and Herbal Medicines on Organic Anion Transporters (Slc22 Family)
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Virginia Commonwealth University VCU Scholars Compass Theses and Dissertations Graduate School 2013 ASSESSMENT OF THE DRUG-DRUG INTERACTION POTENTIAL OF ANIONIC COMPONENTS IN THE DIET AND HERBAL MEDICINES ON ORGANIC ANION TRANSPORTERS (SLC22 FAMILY) Li Wang Virginia Commonwealth University Follow this and additional works at: https://scholarscompass.vcu.edu/etd Part of the Pharmacy and Pharmaceutical Sciences Commons © The Author Downloaded from https://scholarscompass.vcu.edu/etd/3181 This Dissertation is brought to you for free and open access by the Graduate School at VCU Scholars Compass. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of VCU Scholars Compass. For more information, please contact [email protected]. © Li Wang, 2013 All Rights Reserved ASSESSMENT OF THE DRUG-DRUG INTERACTION POTENTIAL OF ANIONIC COMPONENTS IN THE DIET AND HERBAL MEDICINES ON ORGANIC ANION TRANSPORTERS (SLC22 FAMILY) A Dissertation submitted in partially fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University By Li Wang Master of Science, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, China, 2007 Director: Douglas H. Sweet, Ph.D. Associate Professor, Department of Pharmaceutics Virginia Commonwealth University Richmond, Virginia, August, 2013 ii ACKNOWLEDGEMENTS It is never easy to make a successful completion of Ph.D. Thanks to the help and support of the kind people around me, I eventually finish my doctoral training and make my dream come to life. Foremost, I would like to express my sincere gratitude to my advisor Dr. Douglas H. Sweet, for the continuous support of my study and research, for his patience, motivation, and enthusiasm. Indeed, his passion in science would definitely encourage me to move forward in future. I would also like to thank Dr. Jürgen Venitz for the consistent help in my research project. His valuable suggestions give me inspirations to enrich my research. I would like to acknowledge Drs. Patricia W. Slattum, H Thomas Karnes, and Satjit S. Brar who serve in my advisory committee and provide me timely guidance and input. A special gratitude I give to Dr. Matthew S. Halquist for his guidance in my bioanalytical work. In addition, many thanks go to all faculties in School of Pharmacy for their help in my coursework. I would also like to thank Ms. Keyetta Tate and Ms. Laura Georgiadis for their kindly help in ordering lab supplies, arranging departmental activities, and providing directions for living in Richmond. I would thank the VCU School of Pharmacy and Graduate School for the financial support. I would like to thank my lab-mates Dr. Aditi Mulgaonkar, Raymond, Lai, and Christine A. Farthing for their help for my study. Thank you to all my fellow and senior Pharmaceutics graduate students for their help and advice. In addition, a special thank you to my roommates and good friends Kan Qian and Chenxiao Da for their support and encouragement. Finally, I would like to thank my parents for their love and unconditional support, and my wife, Xiaolei Pan, who is always there to cheer me up and encourage me to overcome the problems emerged in my life. iii TABLE OF CONTENTS ACKNOWLEDGEMENTS…………………………………………………………………….ii LIST OF TABLES……………………………………………………………………………...ix LIST OF FIGURES……………………………………………………………………….……xi ABBREVIATIONS………………………………………………………………………..…..xiv ABSTRACT……………………………………………………………………….……….....xviii CHAPTERS 1. RENAL ORGANIC ANION TRANSPORTERS (SLC22 FAMILY): EXPRESSION, REGULATION, ROLES IN TOXICITY, AND IMPACT ON INJURY AND DISEASE…………………………………………………………………..1 1.A INTRODUCTION………………………………………………………………………1 1.B ORGANIC ANION TRANSPORTERS IN RENAL PROXIMAL TUBULE………5 1.C REGULATION OF OAT EXPRESSION……………………………………………..7 1.D OATs IN RENAL INJURY AND PROTECTION…………………………………..12 1.E OAT FUNCTION AND EXPRESSION IN MODELS OF RENAL INJURY AND DISEASE………………………………………………………………………...17 1.F OATs AND DRUG-DRUG INTERACTIONS…………………………………..…...22 1.G CONCLUSIONS……………………………………………………………………….26 2. RESEARCH HYPOTHESES AND SPECIFIC AIMS……………………………….......27 2.A HYPOTHESES………………………………………………………………………..27 2.B SPECIFIC AIMS TO ADDRESS THE ABOVE HYPOTHESES…………………28 3. ACTIVE HYDROPHILIC COMPONENTS OF THE MEDICINAL HERB SALVIA MILTIORRHIZA (DANSHEN) POTENTLY INHIBIT ORGANIC ANION TRANSPORTERS 1 (SLC22A6) and 3 (SLC22A8) ………………………………………..29 iv 3.A INTRODUCTION…………………………………………………………………….29 3.B MATERIALS AND METHODS……………………………………………………..33 3.B.1 Purified chemicals……………………………………………………………….33 3.B.2 Tissue culture……………………………………………………………………33 3.B.3 Cell accumulation assays………………………………………………………..33 3.B.4 Statistics…………………………………………………………………………36 3.C RESULTS………………………………...………………………………………………..37 3.C.1 Inhibition of mOat1 and mOat3 by hydrophilic Danshen components………….37 3.C.2 Determination of the type of inhibition induced by Danshen components on mOat1 and mOat3…………………………………………………………………..39 3.C.3 Inhibition potencies of LSA, RMA, and SAA…………………………………...39 3.D DISCUSSION………………………………………………………………………… 46 4. COMPETITIVE INHIBITION OF HUMAN ORGANIC ANION TRANSPORTERS 1 (SLC22A6) AND 3 (SLC22A8) BY MAJOR COMPONENTS OF THE MEDICINAL HERB SALVIA MILTIORRHIZA (DANSHEN) …………………………………………...51 4.A INTRODUCTION……………………………………………………………………..51 4.B MATERIALS AND METHODS……………………………………………………...57 4.B.1 Chemicals………………………………………………………………………… 57 4.B.2 Tissue culture……………………………………………………………………...57 4.B.3 Cell accumulation assays………………………………………………………….57 4.B.4 Statistics…………………………………………………………………………...58 4.C RESULTS…………………………………………………………………………….…59 4.C.1 Inhibitory effects of hydrophilic Danshen components on hOAT1 and hOAT3 function………………………………………………………………………….59 4.C.2 Mode of inhibition…………………………………………………………………61 4.C.3 Determination of inhibition potencies of LSA, RMA, and SAA for hOAT1 v and hOAT3……………………………………………………………………………….61 4.D DISCUSSION………………………………………………………………………...…70 5. POTENTIAL FOR FOOD/DRUG-DRUG INTERACTIONS BY PHENOLIC ACIDS ON HUMAN ORGANIC ANION TRANSPORTERS 1 (SLC22A6), 3 (SLC22A8), AND 4 (SLC22A11) ……...…………………………………………………… 75 5.A INTRODUCTION…………………………………………………………………...…75 5.B MATERIALS AND METHODS………………………………………………..……..79 5.B.1 Chemicals………………………………………………………………………… 79 5.B.2 Tissue culture…………………………………..………………………………….79 5.B.3 Cell accumulation assay……………………………………………...……………81 5.B.4 Statistics……………………………………………………………………….…..82 5.C RESULTS……………...………………………………………………………………..83 5.C.1 Inhibitory effects of phenolic acids on hOAT1-mediated PAH uptake………….. 83 5.C.2 Inhibitory effects of phenolic acids on hOAT3-mediated ES uptake……………..86 5.C.3. Inhibitory effects of hydrophilic Danshen components on hOAT4-mediated ES uptake……………………………………………………………. 87 5.D DISCUSSION…………..………………………………………………………………92 6. INTERACTION OF NATURAL DIETARY AND HERBAL ANIONIC COMPOUNDS AND FLAVONOIDS WITH HUMAN ORGANIC ANION TRANSPORTERS 1 (SLC22A6), 3 (SLC22A8), AND 4 (SLC22A11)……………………...98 6.A INTRODUCTION………………………………………………………………………98 6.B MATERIALS AND METHODS……………………………………………………..102 6.B.1 Purified chemicals……………………………………………………………….102 6.B.2 Cell culture………………………………………………………………………102 6.B.3 Cell accumulation assays……………………………………………………….. 102 vi 6.B.4 Statistics………………………………………………………………………… 104 6.C RESULTS……………………………………………………………………………...105 6.C.1 Inhibition of hOAT1 by natural anionic compounds and flavonoids……………105 6.C.2 Inhibition of hOAT3 by natural anionic compounds and flavonoids……………108 6.C.3 Inhibition of hOAT4 by natural anionic compounds and flavonoids……………108 6.D DISCUSSION……………………………………………………………………….....111 7. SIMULTANEOUS DETERMINTION OF GALLIC ACID AND GENTISIC ACID IN ORGANIC ANION TRANSPORTER EXPRESSING CELLS BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY…………...115 7.A INTRODUCTION…………………………………………………………………… 115 7.B MATERIAL AND METHODS………………………………………………………118 7.B.1 Chemicals and reagent…………………………………………..………………… 118 7.B.2 Instrumentation……………………………………………….…………………….118 7.B.3 LC-MS/MS condition………………………………………………………………118 7.B.4 Cell culture………………………………………………………………………… 120 7.B.5 Sample preparation…………………………………………………………………120 7.B.6 Calibration and validation………………………………………………………… .121 7.B.7 Matrix effects evaluation…………………………………………………………...122 7.B.8 Application to cellular uptake study……………………………………………..…122 7.C RESULTS AND DISCUSSION………………………………………………………123 7.C.1 LC-MS/MS…………………………………………………………………………123 7.C.2 Validation of the analytical method……………………………...…………………123 7.C.2.1 Linearity and specificity……………………...………………...………………... 123 7.C.2.2 Accuracy and precision………………………………………………………...…124 7.C.2.3 Extraction recovery………………………………..…………...…………………125 vii 7.C.2.4 Matrix effects……………………………………………………………………..130 7.2.C.5 Stability………………………………………………………………….………..130 7.C.3 Determination of intracellular concentration of gallic acid and gentisic acid in OAT- expressing cells……………………………………………………………………………. 131 7.D CONCLUSIONS……………………………………………………..………………...134 8. SYSTMEIC LEVEL EVALUATION OF ORGANIC ANION TRANSPORTERS- MEDIATED DRUG-DRUG INTERACTION POTENCY IN SALVIA MITIORRHIZA (DANSHEN) PREPARATIONS: FOCUSING ON CUMULATIVE EFFECTS FROM MULTIPLE COMPONENTS……………………… 135 8.A INTRODUCTION………………………………………………..……………………135 8.B MATERIALS AND METHODS……………………………...………………………139 8.B.1 Materials……………………….…………………………………………………139 8.B.2 Tissue culture………………….……………………….…………………………139 8.B.3 Cell accumulation assays………………….…………………………...…………139 8.B.4 Derivation of cumulative DDI index……………..…...………………………… .140 8.B.5 Pharmacokinetic modeling .…………………………...………………………… 140 8.B.6 Simulation