AXIN2 Antibody Cat. No.: 6163
Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 μg/mL.
Immunohistochemistry of AXIN2 in rat lung tissue with Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2 AXIN2 antibody at 5 μg/mL. antibody at 20 μg/mL.
Immunofluorescence of AXIN2 in mouse lung tissue with AXIN2 antibody at 20 μg/mL. Immunohistochemistry of AXIN2 in mouse lung tissue with AXIN2 antibody at 5 μg/mL. Red: AXIN2 Antibody (6163) Blue: DAPI staining
September 28, 2021 1 https://www.prosci-inc.com/axin2-antibody-6163.html Immunofluorescence of AXIN2 in HepG2 cells with AXIN2 Antibody at 20 μg/ml.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (85%)
AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2. IMMUNOGEN:
The immunogen is located within amino acids 780 - 830 of AXIN2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 SPECIFICITY: are known to exist; this antibody will recognize all four.
POSITIVE CONTROL: 1) Cat. No. 1402 - Mouse Lung Tissue Lysate
PREDICTED MOLECULAR 85, 93 kDa WEIGHT:
Properties
PURIFICATION: AXIN2 Antibody is affinity chromatography purified via peptide column.
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
September 28, 2021 2 https://www.prosci-inc.com/axin2-antibody-6163.html PHYSICAL STATE: Liquid
BUFFER: AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION: 1 mg/mL
AXIN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: AXIN2
ALTERNATE NAMES: AXIN2 Antibody: AXIL, ODCRCS, Axin-2, Axin-like protein, Axil
ACCESSION NO.: AAF22799
PROTEIN GI NO.: 195927059
GENE ID: 8313
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
AXIN2 Antibody: Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway. In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation BACKGROUND: of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair.
1) Katoh M and Katoh M. WNT signaling pathway and stem cell signaling network. Clin. REFERENCES: Cancer Res.2007; 13:4042-5.
2) Behrens J, Jerchow BA, Wurtele M, et al. Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta. Science1998; 280:596-9.
3) Hadjihannas MV and Behrens J. CIN by WNT: growth pathways, mitotic control and chromosome instability in cancer. Cell Cycle2006; 5:2077-81.
4) Mai M, Qian C, Yokomizo A, et al. Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24. Genomics1999; 55:341-4.
ANTIBODIES FOR RESEARCH USE ONLY.
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