AXIN2 Antibody Cat. No.: 6163

Western blot analysis of AXIN2 in mouse lung lysate with AXIN2 antibody at 1 μg/mL.

Immunohistochemistry of AXIN2 in rat lung tissue with Immunofluorescence of AXIN2 in Rat Lung cells with AXIN2 AXIN2 antibody at 5 μg/mL. antibody at 20 μg/mL.

Immunofluorescence of AXIN2 in mouse lung tissue with AXIN2 antibody at 20 μg/mL. Immunohistochemistry of AXIN2 in mouse lung tissue with AXIN2 antibody at 5 μg/mL. Red: AXIN2 Antibody (6163) Blue: DAPI staining

September 28, 2021 1 https://www.prosci-inc.com/axin2-antibody-6163.html Immunofluorescence of AXIN2 in HepG2 cells with AXIN2 Antibody at 20 μg/ml.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (85%)

AXIN2 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human AXIN2. IMMUNOGEN:

The immunogen is located within amino acids 780 - 830 of AXIN2.

TESTED APPLICATIONS: ELISA, IF, IHC-P, WB

AXIN2 antibody can be used for detection of AXIN2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

AXIN2 antibody is predicted to not cross-react with AXIN1. At least four isoforms of AXIN2 SPECIFICITY: are known to exist; this antibody will recognize all four.

POSITIVE CONTROL: 1) Cat. No. 1402 - Mouse Lung Tissue Lysate

PREDICTED MOLECULAR 85, 93 kDa WEIGHT:

Properties

PURIFICATION: AXIN2 Antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

September 28, 2021 2 https://www.prosci-inc.com/axin2-antibody-6163.html PHYSICAL STATE: Liquid

BUFFER: AXIN2 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

AXIN2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: AXIN2

ALTERNATE NAMES: AXIN2 Antibody: AXIL, ODCRCS, Axin-2, Axin-like , Axil

ACCESSION NO.: AAF22799

PROTEIN GI NO.: 195927059

GENE ID: 8313

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

AXIN2 Antibody: Like the related protein AXIN1, AXIN2 is thought to play an important role in the regulation of the stability of beta- in the . In mouse, AXIN2 organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and AXIN2, which leads to the degradation BACKGROUND: of beta-catenin, which is thought to be an important event in the genesis of a number of malignancies. The AXIN2 has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with with defective mismatch repair.

1) Katoh M and Katoh M. WNT signaling pathway and stem cell signaling network. Clin. REFERENCES: Cancer Res.2007; 13:4042-5.

2) Behrens J, Jerchow BA, Wurtele M, et al. Functional interaction of an axin homolog, conductin, with beta-catenin, APC, and GSK3beta. Science1998; 280:596-9.

3) Hadjihannas MV and Behrens J. CIN by WNT: growth pathways, mitotic control and instability in cancer. Cell Cycle2006; 5:2077-81.

4) Mai M, Qian C, Yokomizo A, et al. Cloning of the human homolog of conductin (AXIN2), a gene mapping to chromosome 17q23-q24. Genomics1999; 55:341-4.

ANTIBODIES FOR RESEARCH USE ONLY.

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