NEW Products for Immunology Research
PROTEINS FOR EMERGING AVI-TAGGED BIOTINYLATED NEW SMALL MOLECULES IMMUNE CHECKPOINT TARGETS RECOMBINANT PROTEINS
APC 16673-34-0 NLRP3i BirA Enzyme Biotin O NH2 Signal 1 S MHC TCR-CD3 Biotin GLNDIFEAQKIEWHE O Complex O Cl N GLNDIFEAQKIEWHE H Signal 2 T Cell Avi Tag O
ANTI-IDIOTYPE AND BIOSIMILAR RECOMBINANT MONOCLONAL CELL MARKERS INTERACTIVE ANTIBODIES ANTIBODIES RESOURCE TOOL
Cell Surface Markers = Intracellular Markers
NEW IMMUNOASSAYS SIMPLE PLEX™ ASSAYS LUMINEX® DISCOVERY FIXED PANELS
SINGLE-CELL WESTERNS RNASCOPE® ISH ASSAYS CELL CULTURE MEDIA & FBS
GMP CLOUDZ™ CELL SELECTION NEW LITERATURE NEW INTERACTIVE PATHWAYS & ACTIVATION KITS Immune Cell Isolation & Culture Quality Results Start at the Core Bio-Techne Immunoassays — Powered by R&D Systems CD4+ T Cell Subsets
Th2
Th1 Th9
Signal 1 B MHCII TCR-CD3 Complex Th17 Signal 2 Naïve CD4+ T Cell APC
Treg Th22
Tfh
Immunology T Y B O O K A C T I V I O M
L A S O Z L P U Z O R D S S W C R O E O I S R N D G F P I A
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E U D O K S The Bio-Techne brands, R&D Systems®, Novus Biologicals®, Tocris®, ProteinSimple®, and ACD™, are always working hard to develop new products that will help to advance your research. Since we release new products weekly and the pace of research never slows down, we realize that it can be difficult to stay up-to-date on what new products or technologies are available. This guide is intended to highlight recently developed Bio-Techne products and resources that we feel are particularly relevant to immunology researchers.
Table of Contents
Bioactive Proteins for Emerging Immune Checkpoint Targets...... 2 Avi-Tagged Biotinylated Recombinant Proteins...... 6 New Tocris Small Molecules for Immunology Research...... 7 Cell Culture Media...... 8 Classic Fetal Bovine Serum...... 9 New R&D Systems and Novus Biologicals Antibodies for Immunology Research...... 10 Cell Markers Interactive Resource Tool...... 12 New Immunoassays for Immunology Research...... 14 Simple Plex™ Assays...... 15 New Luminex® Performance Discovery Panels...... 16 Single-Cell Westerns...... 17 RNAscope® ISH Assays for Inflammation, Immuno-Oncology, and Viral Pathogen Research...... 17 GMP-grade Immune Cell Culture Reagents...... 18
2 Bioactive Proteins for Emerging Immune Checkpoint Targets
Tumor cells frequently overexpress B7 family proteins that negatively regulate T cell activation, which allows them to evade detection by the host’s immune system. As a result, researchers have focused on targeting these negative regulatory immune checkpoint pathways for cancer immunotherapy. Utilizing monoclonal antibodies directed against the T cell co-inhibitory receptors, CTLA-4, PD-1, or the PD-1 ligand, PD-L1, clinicians have seen remarkable success in some cancer patients. Unfortunately, they have also found that these antibodies are only effective in a minority of patients and some patients that initially show a positive response to treatment with these antibodies, can become resistant or relapse due to an upregulation of other immune checkpoint pathways. Therefore, additional immune checkpoint regulators that may serve as immunotherapeutic targets, either alone or in combination, are being sought. Bio-Techne is focused on developing new reagents for investigating potential next generation immune checkpoint targets. Our current portfolio includes R&D Systems bioactive recombinant proteins for studying members of the Butyrophilin, VSTM, VSIG, CEACAM, AMIGO, and SIRP protein families.
Butyrophilins
Butyrophilins are a novel class of co-stimulatory/co-inhibitory molecules that are structurally related to the B7 family and appear to have similar immunomodulatory functions. To date, 13 human butyrophilin proteins have been described, with most acting through unidentified receptors to inhibit T cell proliferation and cytokine production. The parallels between the butyrophilin proteins and the B7 family suggest that these proteins may also serve as drug targets for immune checkpoint blockade.
Butyrophilins Butyrophilins Molecule Species Source Tag Cat # Molecule Species Source Tag Cat # Human NS0 None 8467-BT BTNL2 Mouse NS0 None 8605-BT BTN1A1 Human NS0 Biotin BT8467 BTNL3 Human CHO Fc 9658-BT Mouse NS0 None 8540-BT Mouse NS0 Fc 9590-BT BTNL4 BTN2A1 Human HEK293 None 9058-BT Mouse NS0 His 9594-BT Human HEK293 Fc 8918-BT BTNL6 Mouse CHO None 9638-BT BTN2A2 Human HEK293 Fc, Biotin BT8918 Human HEK293 Fc 9359-BT BTNL8 Mouse NS0 None 8997-BT Human HEK293 Fc, Biotin BT9359 Human HEK293 Fc 8539-BT Human HEK293 Fc 9659-BT BTN3A1/CD277 BTNL9 Human HEK293 Fc, Biotin BT8539 Mouse NS0 Fc 9555-BT Human CHO None 9514-BT Human HEK293 Fc 10014-BT BTN3A2 BTNL10 Human CHO Biotin BT9514 Mouse NS0 Fc 1444-BT
BTN3A1 Inhibits Anti-CD3-induced IL-2 Production by A B Human T Cells in a Manner Similar to B7-H1/PD-L1. Human T cells were incubated with an immobilized Mouse Anti-Human CD3e Monoclonal Antibody (R&D Systems, Catalog # MAB100; 1 µg/mL) and the indicated ) R NR ) R NR concentrations of (A) Recombinant Human BTN3A1/ 4 0 4 0 R NR CD277 Fc Chimera (R&D Systems, Catalog # 8539-BT) or 190 (B) Recombinant Human B7-H1/PD-L1 Fc Chimera (R&D Systems, Catalog # 156-B7). IL-2 secretion was 92.5 measured in cell culture supernatants using the Human 66 ® 55 IL-2 Quantikine ELISA Kit (R&D Systems, Catalog # 43 D2050). The ED50 for this effect is typically 1-5 µg/mL for 36 Recombinant Human BTN3A1/CD277 and 2-10 µg/mL for 29
IL-2 Production (O.D. IL-2 Production (O.D. IL-2 Production Recombinant Human B7-H1/PD-L1. The purity of (A) 21 18.4 Recombinant Human BTN3A1/CD277 (1 µg/lane) and (B) 12.4 Recombinant Human B7-H1/PD-L1 (1 µg/lane) was 6.3 assessed by SDS-PAGE analysis under reducing (R) and non-reducing (NR) conditions and visualized by silver staining. Recombinant Human BTN3A1/Fc ( g/mL) Recombinant Human B7-H1/Fc ( g/mL)
Learn more | rndsystems.com/butyrophilins and rndsystems.com/butyrophilinwebinarshort 3 VSTM Family Proteins
There are 8 members of the V-set and transmembrane domain-containing (VSTM) protein family, which are structurally related to the B7 family proteins. R&D Systems scientists have found that many of these proteins have the R NR ability to suppress anti-CD3-induced cytokine secretion by human T cells, suggesting that they may also play a role in regulating T cell functions in vivo. VSTM Family Proteins Molecule Species Source Tag Catalog # Production (Mean O.D.) γ Production Human HEK293 His 10037-VT VSTM2A IFN- Mouse NS0 His 2283-VT Human HEK293 Fc 10060-VT VSTM2B Human HEK293 His 10061-VT Recombinant Human VSTM4 ( g/mL) Mouse NS0 Fc 10076-VT VSTM4 Inhibits Anti-CD3-induced IFN-g Production by Human CHO HA 2111-VT VSTM2L Human T Cells. Human peripheral blood mononuclear Mouse CHO HA 2152-VT cells were incubated with an immobilized Mouse Anti-Human CD3e Monoclonal Antibody (R&D Systems, Human CHO Fc 7898-TGB Catalog # MAB100) and the indicated concentrations of Human CHO His 9525-TG Recombinant Human VSTM4 Fc Chimera (R&D Systems, Catalog # 2086-VT). IFN-g secretion was measured in cell VSTM3/TIGIT/VSIG9 Human HEK293 Fc 9464-TG culture supernatants using the Human IFN-g Quantikine®
Cynomolgus Monkey HEK293 Fc 9380-TG ELISA Kit (R&D Systems, Catalog # DIF50). The ED50 for this effect is typically 1-10 µg/mL. The purity of Mouse NS0 Fc 7267-TG Recombinant Human VSTM4 (2 µg/lane) was assessed by VSTM4 Human CHO Fc 2086-VT SDS-PAGE analysis under reducing (R) and non-reducing (NR) conditions and visualized by silver staining.
VSIG Family Proteins
Several members of the V-set and immunoglobulin domain-containing (VSIG) protein family are now recognized as potential immuno-oncology targets. The most well-known member of this family, VSIG9/TIGIT/VSTM3, has been shown to have both T cell and natural killer cell inhibitory functions. Additionally, VSIG4 has been reported to negatively regulate T cell proliferation and IL-2 production and inhibit pro-inflammatory macrophage activation, while VSIG3 has been shown to inhibit human T cell functions through binding to the B7 family member, VISTA/PD-1H. Our own unpublished observations indicate that other VSIG proteins also negatively regulate T cell functions.
VSIG Family Proteins VSIG Family Proteins Molecule Species Source Tag Cat. # Molecule Species Source Tag Cat. # Human NS0 Fc 9708-VS VSIG4 Short Human CHO His 9816-VS VSIG1 Mouse NS0 Fc 9645-VS Isoform Human CHO Fc 9817-VS Human NS0 Fc 9815-VS Human CHO Fc 9200-VS VSIG2 Mouse NS0 Fc 9598-VS Fc, VSIG8 Human CHO BT9200 Biotin Human NS0 Fc 9229-VS VSIG3 Mouse NS0 Fc 9204-VS
Mouse NS0 Fc 9367-VS (RF ) T Cell Proliferation Human CHO Fc 7898-TGB VSIG3 Human CHO Fc 10074-VS N-Terminus Human CHO His 9525-TG VSIG9/ VSIG3 Human HEK293 Fc 9464-TG Human CHO Fc 10075-VS TIGIT/ VSIG3 IgG -Fc ( g/mL) C-Terminus 1 VSTM3 Cynomolgus HEK293 Fc 9380-TG VSIG3 Inhibits Anti-CD3-induced Human CD3+ T Cell Human NS0 Fc 4646-VS Monkey Proliferation. Human T cells were incubated with an Fc, Mouse NS0 Fc 7267-TG immobilized Mouse Anti-Human CD3e Monoclonal Antibody Human NS0 BT4646 Biotin (R&D Systems, Catalog # MAB100; 1 µg/mL) and the VSIG10 Mouse NS0 Fc 9604-VS VSIG4 indicated concentrations of Recombinant Human VSIG3 Fc Human NS0 His 9678-VS Chimera (R&D Systems, Catalog # 9229-VS). Cell Mouse NS0 Fc 4674-VS proliferation was assessed by a fluorometric assay using the redox-sensitive dye, Resazurin (R&D Systems, Catalog Mouse NS0 His 9747-VS # AR002). IgG-Fc controls did not alter anti-CD3-induced CD3+ T cell proliferation (data not shown). Learn more | rndsystems.com/VSIG8poster and rndsystems.com/VSIG3-VISTAposter 4 CEACAM Proteins
Carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) are part of the CEA protein family consisting of CEACAMs and the pregnancy-specific glycoproteins (PSGs). These proteins are part of the immunoglobulin superfamily and have a wide variety of different functions in cell adhesion, cell signaling, inflammation, angiogenesis, and cancer. They have been shown to bind to each other and have been described as receptors for TIM-3, galectins, and integrins. Additionally, CEACAM-1, CEACAM-5, and CEACAM-6 are now considered to be cancer biomarkers and potential targets for cancer immunotherapy.
CEACAM Proteins Molecule Species Source Tag Catalog #
CEACAM-1/CD66a Human NS0 His 2244-CM Human NS0 His 9868-CM CEACAM-3/CD66d Human CHO Fc 9985-CM CEACAM-5/CD66e Human NS0 His 4128-CM CEACAM-6/CD66c Human NS0 His 3934-CM CEACAM-7 Human CHO His 9010-CM Galectin-3 Binding (Mean O.D.) CEACAM-8/CD66b Human NS0 His 9639-CM
CEACAM-16 Human CHO HA 9885-CM CEACAM-18 Human HEK293 Fc 9869-CM Recombinant Human CEACAM-21 ( g/mL) CEACAM-19 Human HEK293 Fc 9397-CM CEACAM-21 Binds to Galectin-3. Recombinant Human CEACAM-20 Human CHO Fc 9427-CM Galectin-3 (R&D Systems, Catalog #8259-GA) was coated at 1 µg/mL, 100 µL/well and the indicated concentrations CEACAM-21 Human CHO Fc 9870-CM of Recombinant Human CEACAM-21 Fc Chimera (R&D Sys- tems, Catalog # 9870-CM) were added. The concentration of Recombinant Human CEACAM-21 Fc Chimera that produces 50% of the optimal binding response is 0.07–0.7 µg/mL. AMIGO Proteins
AMIGO family proteins (AMIGO1-3) are cell adhesion molecules that exhibit homophilic and heterophilic binding properties. Relatively little is known about their functions, but our in-house data shows that AMIGO2 and AMIGO3 inhibit anti-CD3-induced IL-2 secretion by CD3+ T cells, suggesting that they may also regulate T cell functions in vivo.
AMIGO Family Proteins Molecule Species Source Tag Catalog # Human NS0 His 2565-AM AMIGO Mouse NS0 His 10084-AM Human CHO Fc 2080-AM AMIGO2 Mouse NS0 Fc 9424-AM Human CHO Fc 9508-AM AMIGO3 Mouse NS0 Fc 9425-AM IL-2 Secretion (Mean O.D.) IL-2 Secretion