NEW Products for Immunology Research PROTEINS FOR EMERGING AVI-TAGGED BIOTINYLATED NEW SMALL MOLECULES IMMUNE CHECKPOINT TARGETS RECOMBINANT PROTEINS APC 16673-34-0 NLRP3i BirA Enzyme Biotin O NH2 Signal 1 S MHC TCR-CD3 Biotin GLNDIFEAQKIEWHE O Complex O Cl N GLNDIFEAQKIEWHE H Signal 2 T Cell Avi Tag O ANTI-IDIOTYPE AND BIOSIMILAR RECOMBINANT MONOCLONAL CELL MARKERS INTERACTIVE ANTIBODIES ANTIBODIES RESOURCE TOOL Cell Surface Markers = Intracellular Markers NEW IMMUNOASSAYS SIMPLE PLEX™ ASSAYS LUMINEX® DISCOVERY FIXED PANELS SINGLE-CELL WESTERNS RNASCOPE® ISH ASSAYS CELL CULTURE MEDIA & FBS GMP CLOUDZ™ CELL SELECTION NEW LITERATURE NEW INTERACTIVE PATHWAYS & ACTIVATION KITS Immune Cell Isolation & Culture Quality Results Start at the Core Bio-Techne Immunoassays — Powered by R&D Systems CD4+ T Cell Subsets Th2 Th1 Th9 Signal 1 B MHCII TCR-CD3 Complex Th17 Signal 2 Naïve CD4+ T Cell APC Treg Th22 Tfh Immunology T Y B O O K A C T I V I O M L A S O Z L P U Z O R D S S W C R O E O I S R N D G F P I A G E U D O K S The Bio-Techne brands, R&D Systems®, Novus Biologicals®, Tocris®, ProteinSimple®, and ACD™, are always working hard to develop new products that will help to advance your research. Since we release new products weekly and the pace of research never slows down, we realize that it can be difficult to stay up-to-date on what new products or technologies are available. This guide is intended to highlight recently developed Bio-Techne products and resources that we feel are particularly relevant to immunology researchers. Table of Contents Bioactive Proteins for Emerging Immune Checkpoint Targets .................................................................................................................2 Avi-Tagged Biotinylated Recombinant Proteins .........................................................................................................................................6 New Tocris Small Molecules for Immunology Research ...........................................................................................................................7 Cell Culture Media .......................................................................................................................................................................................8 Classic Fetal Bovine Serum ........................................................................................................................................................................9 New R&D Systems and Novus Biologicals Antibodies for Immunology Research ............................................................................... 10 Cell Markers Interactive Resource Tool .................................................................................................................................................. 12 New Immunoassays for Immunology Research ..................................................................................................................................... 14 Simple Plex™ Assays ................................................................................................................................................................................. 15 New Luminex® Performance Discovery Panels ...................................................................................................................................... 16 Single-Cell Westerns .................................................................................................................................................................................17 RNAscope® ISH Assays for Inflammation, Immuno-Oncology, and Viral Pathogen Research ..............................................................17 GMP-grade Immune Cell Culture Reagents ............................................................................................................................................ 18 2 Bioactive Proteins for Emerging Immune Checkpoint Targets Tumor cells frequently overexpress B7 family proteins that negatively regulate T cell activation, which allows them to evade detection by the host’s immune system. As a result, researchers have focused on targeting these negative regulatory immune checkpoint pathways for cancer immunotherapy. Utilizing monoclonal antibodies directed against the T cell co-inhibitory receptors, CTLA-4, PD-1, or the PD-1 ligand, PD-L1, clinicians have seen remarkable success in some cancer patients. Unfortunately, they have also found that these antibodies are only effective in a minority of patients and some patients that initially show a positive response to treatment with these antibodies, can become resistant or relapse due to an upregulation of other immune checkpoint pathways. Therefore, additional immune checkpoint regulators that may serve as immunotherapeutic targets, either alone or in combination, are being sought. Bio-Techne is focused on developing new reagents for investigating potential next generation immune checkpoint targets. Our current portfolio includes R&D Systems bioactive recombinant proteins for studying members of the Butyrophilin, VSTM, VSIG, CEACAM, AMIGO, and SIRP protein families. Butyrophilins Butyrophilins are a novel class of co-stimulatory/co-inhibitory molecules that are structurally related to the B7 family and appear to have similar immunomodulatory functions. To date, 13 human butyrophilin proteins have been described, with most acting through unidentified receptors to inhibit T cell proliferation and cytokine production. The parallels between the butyrophilin proteins and the B7 family suggest that these proteins may also serve as drug targets for immune checkpoint blockade. Butyrophilins Butyrophilins Molecule Species Source Tag Cat # Molecule Species Source Tag Cat # Human NS0 None 8467-BT BTNL2 Mouse NS0 None 8605-BT BTN1A1 Human NS0 Biotin BT8467 BTNL3 Human CHO Fc 9658-BT Mouse NS0 None 8540-BT Mouse NS0 Fc 9590-BT BTNL4 BTN2A1 Human HEK293 None 9058-BT Mouse NS0 His 9594-BT Human HEK293 Fc 8918-BT BTNL6 Mouse CHO None 9638-BT BTN2A2 Human HEK293 Fc, Biotin BT8918 Human HEK293 Fc 9359-BT BTNL8 Mouse NS0 None 8997-BT Human HEK293 Fc, Biotin BT9359 Human HEK293 Fc 8539-BT Human HEK293 Fc 9659-BT BTN3A1/CD277 BTNL9 Human HEK293 Fc, Biotin BT8539 Mouse NS0 Fc 9555-BT Human CHO None 9514-BT Human HEK293 Fc 10014-BT BTN3A2 BTNL10 Human CHO Biotin BT9514 Mouse NS0 Fc 1444-BT BTN3A1 Inhibits Anti-CD3-induced IL-2 Production by A B 0.7 0.7 Human T Cells in a Manner Similar to B7-H1/PD-L1. Human T cells were incubated with an immobilized Mouse 0.6 0.6 Anti-Human CD3e Monoclonal Antibody (R&D Systems, Catalog # MAB100; 1 µg/mL) and the indicated ) R NR ) R NR kDa concentrations of (A) Recombinant Human BTN3A1/ 40 0.5 190 40 0.5 R NR kDa CD277 Fc Chimera (R&D Systems, Catalog # 8539-BT) or 190 190 (B) Recombinant Human B7-H1/PD-L1 Fc Chimera 0.4 92.5 0.4 (R&D Systems, Catalog # 156-B7). IL-2 secretion was 92.5 66 92.5 measured in cell culture supernatants using the Human 55 66 66 ® 0.3 43 0.355 55 IL-2 Quantikine ELISA Kit (R&D Systems, Catalog # 36 43 43 D2050). The ED50 for this effect is typically 1-5 µg/mL for 29 36 36 Recombinant Human BTN3A1/CD277 and 2-10 µg/mL for 0.2 0.229 29 21 IL-2 Production (O.D. IL-2 Production (O.D. IL-2 Production Recombinant Human B7-H1/PD-L1. The purity of (A) 18.4 21 21 18.4 18.4 Recombinant Human BTN3A1/CD277 (1 µg/lane) and (B) 0.1 12.4 0.1 12.4 12.4 Recombinant Human B7-H1/PD-L1 (1 µg/lane) was 6.3 6.3 6.3 assessed by SDS-PAGE analysis under reducing (R) and 0 0 non-reducing (NR) conditions and visualized by silver 0.001 0.01 0.1 1 10 100 0.001 0.01 0.1 1 10 100 staining. Recombinant Human BTN3A1/Fc (g/mL) Recombinant Human B7-H1/Fc (g/mL) Learn more | rndsystems.com/butyrophilins and rndsystems.com/butyrophilinwebinarshort 3 VSTM Family Proteins There are 8 members of the V-set and transmembrane domain-containing 1.2 (VSTM) protein family, which are structurally related to the B7 family proteins. 1.0 R&D Systems scientists have found that many of these proteins have the kDa R NR ability to suppress anti-CD3-induced cytokine secretion by human T cells, 0.8 190 suggesting that they may also play a role in regulating T cell functions in vivo. 0.6 92 VSTM Family Proteins 66 Molecule Species Source Tag Catalog # 0.4 55 Production (Mean O.D.) Production 43 γ Human HEK293 His 10037-VT 36 29 VSTM2A IFN- 0.2 21 Mouse NS0 His 2283-VT 18 12 Human HEK293 Fc 10060-VT 6 0 VSTM2B Human HEK293 His 10061-VT 10-2 10-1 100 101 102 Recombinant Human VSTM4 (g/mL) Mouse NS0 Fc 10076-VT VSTM4 Inhibits Anti-CD3-induced IFN-g Production by Human CHO HA 2111-VT VSTM2L Human T Cells. Human peripheral blood mononuclear Mouse CHO HA 2152-VT cells were incubated with an immobilized Mouse Anti-Human CD3e Monoclonal Antibody (R&D Systems, Human CHO Fc 7898-TGB Catalog # MAB100) and the indicated concentrations of Human CHO His 9525-TG Recombinant Human VSTM4 Fc Chimera (R&D Systems, Catalog # 2086-VT). IFN-g secretion was measured in cell ® VSTM3/TIGIT/VSIG9 Human HEK293 Fc 9464-TG culture supernatants using the Human IFN-g Quantikine Cynomolgus Monkey HEK293 Fc 9380-TG ELISA Kit (R&D Systems, Catalog # DIF50). The ED50 for this effect is typically 1-10 µg/mL. The purity of Mouse NS0 Fc 7267-TG Recombinant Human VSTM4 (2 µg/lane) was assessed by VSTM4 Human CHO Fc 2086-VT SDS-PAGE analysis under reducing (R) and non-reducing (NR) conditions and visualized by silver staining. VSIG Family Proteins Several members of the V-set and immunoglobulin domain-containing (VSIG) protein family