All new Comprehensive image-analysis tools SigmaScan Pro specifications version 5.0

Input options Spatial measurements Data plotting Output options for a wide range of applications ■ Capture images with any TWAIN ■ Perimeter, area, shape factor, ■ Y vs. row number, Y vs. X, ■ Save image: BMP, TIFF, PCX compatible device or frame compactness, feret diameter, multiple Y vs. X and JPEG ■ ■ Powerful grabber board number of pixels, center of mass, Regression lines Save data: SigmaPlot, Lotus, ■ Open image files TIFF, TGA, TCX, major/minor axes length, slope, ■ Grid lines Excel, ASCII BMP and JPEG end points, and volume for ■ Scatterplot, line plot, symbols ■ Save session (.SES) ■ Load 1, 4, 8, 16, 24 or 32-bit axially symmetric objects and lines plot ■ Print: images, worksheets, plots color images ■ Spatial calibration: ■ Graph and axes titles ■ ■ image analysis Load 1, 4 and 8-bit 1, 2 and 3 point Linear or logarithmic scaling Archeology Electrical grayscale images ■ Intensity calibration: Collect fossil engineering ■ Open data files SigmaPlot, Lotus, linear and nonlinear measurements Printed circuit Excel, Quattro, DBF, DIF ■ Copy calibration data and ASCII ■ Fill holes ■ Systat , Inc. offers a wide range of for your PC easily and board design, Open saved SigmaScan Pro accurately from analysis and sessions (.SES) Intensity measurements software solutions for scientists and engineers ■ Average over an area, photographs of annotation is ¨ Image editing line width average, pixel ■ SYSTAT, Unparalleled research-quality statistics image files. simplified from ■ Cut, copy and paste intensity, total intensity, hue, ■ Crop, duplicate and restore and saturation and graphics ■ ¨ a photo or ■ Image information ■ Line measurements: slope, SigmaPlot, Exact graphs for exact science scanned image. angle, distance ■ SigmaStat,¨ Advisory statistical companion to SigmaPlot Image annotation ■ Point measurements: ■ SigmaScan¨ Pro, Powerful image analysis Exact positions ■ Text, lines, arrows, rectangles, tally, XY coordinates ■ TableCurve 2D and 3D,ª Automated equation and connec- ovals, concentric circles, grids, ■ Object ID numbers fill, unfill, erase ■ Object number reporting discovery and curve fitting tions are easy ■ ª ■ Multiple pen sizes PeakFit, Automatically separate and fit up to 100 peaks to measure. ■ Undo Layer (binary) functions ■ AutoSignal,ª Easy signal analysis ■ Image masking ■ Four non-destructive overlay

and reverse masking layer colors ■ Object elimination ■ Multiple thresholds per image ■ Binary filters Image processing ■ Erosion: normal, split objects, Systat Software, Inc. ■ Color to monochrome preserve shapes, keep residuals ■ Biology Electron conversions Dilation: normal, don’t 501 Canal Boulevard, Suite E Obtain diagnosis microscopy ■ Monochrome Lookup Tables merge objects, preserve shapes, (LUTs) dilate residues Richmond, CA 94084-2028 of disease Obtain accurate ■ Pseudo-color LUTs ■ Special Tel: 1-800-797-7401 from accurate size and area ■ Clearfield equalization ■ Delete single pixels, save object Fax: 510-231-4789 ■ Pseudo-clearfield equalization edges, delete all objects touching Email: sales@.com leaf color measurements ■ Image math the image edge ■ www.systat.com measurements. from video (add, subtract, average) Layer math: AND, OR, XOR, ■ Image splice NOT, copy, clear input of ■ Image rotation: ■ Object labeling detailed 90, 180, 270 degrees ■ Major/Minor axes, structures. ■ Image flipping: along horizontal object number axis, along vertical axis ■ Mask image Data handling ■ Intensity histogram ■ Data worksheet: 65,000 rows Visit us at www.systat.com ■ Histogram stretch and 16,000 columns ■ Convert to grayscale ■ Column statistics ■ Color thresholding ■ Mean, std. deviation, std. error, ■ Gray filters 95% and 99% confidence ■ Median, ranking, intervals, size, total, min/max average, gradient value, min. positive value, Chemistry Arial/Satellite ■ Prewitt (East & North), Laplace missing values Measure particle photography (4 & 8), Sobel (magnitude and Transforms phase), Roberts, user defined ■ Create user-defined transforms size and shape Accurately using over 50 math functions from microscopic determine areas View options (trigonometric, number, range, slides or micro- or distances from ■ Zoom in accumulation, random number ■ Zoom out generator, precision, statistical, graphs. Here, the photographs, and ■ Magnification tool: from 1 (no area, distance and more) size data is fitted highlight specific zoom) to 32 (32X normal size) ■ Fast Fourier transforms (FFT): Measurements object classify with TableCurve areas, such as ■ Measure defined 2D to obtain an roads and (automatic measurements) ■ Trace measurement options exact curve fit. greenery. ■ Fill measurement options ¨ ■ © Copyright 2005 Systat Software, Inc. SigmaScan, SigmaPlot, SigmaStat, SYSTAT, SigmaScan Pro 5.0 Edge/Line tracking options TableCurve 2D, TableCurve 3D, PeakFit and AutoSignal are registered trademarks of ■ Fast object count Systat Software, Inc. All other names are trademarks of their respective owners. for Windows Improve images with A black and white image of An imaging task: from start to finish Easily count, measure and analyze sophisticated filtering Jupiter is contrast SigmaScan Pro’s many image-enhancement enhanced and with SigmaScan Pro tools make it easy to clean up and improve colorized with pseudo-color your digital images your digital images. You can remove noise or Lookup Tables. sharpen contrast with the built-in filters You can clearly STEP Open the image or capture it STEP Select the desired measurement tools (convolution, ranking, binary and histogram see the moon, Io, igmaScan Pro provides a complete image-analysis package for studying in the image remapping) for better clarity. magnifier. 1 4 the structure and size of visual information. SigmaScan Pro’s powerful Use Image Math to remove noise and image analysis, enhancement and expert manipulation techniques highlight features by adding, subtracting or S averaging the pixel intensities of different transform images into reliable statistics, understandable graphs and valuable images. With Lookup Tables, you can add scientific conclusions. color patterns to your monochrome images or convert color images to grayscale. SigmaScan Pro also includes image processing procedures, such as clearfield equalization, pseudo-color and binary filters to clearly define image highlights.

Easily manipulate and annotate A stained microscope your images specimen of SigmaScan Pro’s four non-destructive overlay cancerous layers let you mark, zoom, paste, clear, mask, growths is analyzed for STEP Enhance the image quality STEP Your results are automatically Turn any image into analytical results and meaningful data and statistics. rotate, flip, annotate or define measurement size and marked on the screen and collected in parameters for specific features. The drawing shape. The 2 5 the spreadsheet tools, including text, boxes, grids, ovals and drawing tools are used to Powerful measurement tools more, are easy to use. Simply choose all tools highlight key Choose from 50 powerful mathematical from the floating Annotation Toolbar. areas for transforms to measure the image objects as To help easily identify image objects, presentation you mark them with points, lines or areas for SigmaScan Pro labels each object either directly and analysis. instant analysis. on screen or in the status bar. To further For automated highlighting of image identify objects and their corresponding objects, use light density or color selection measurements, use Object Number Reporting, from the analysis histogram. SigmaScan Pro’s picking out unwanted image objects from the powerful edge and line tracking option spreadsheet. One mouse click cleans up your automatically traces the edge of an object and analysis, removing unwanted objects and collects running measurements, so you save measurements. And, to ensure consistent time marking the desired items. And, to analysis, the spatial and intensity calibration automate repetitive tasks, use SigmaScan information transfers between images. Pro’s built-in Keyboard Macro Recorder. With SigmaScan Pro, you can automatically STEP Calibrate the distance with the STEP Use the column statistics and graphing count, label, and measure up to 64,000 Advanced Develop solid conclusions with measurement tool capabilities to turn data into conclusions objects at a time. You can also collect area, features include advanced data analytical capabilities 3 6 perimeter, slope, major or minor axis, shape column statistics, Use color or histogram SigmaScan Pro enables you to program your task macro factor, angles and many other measurements thresholding to select own enhancements, transforms and recorder, simultaneously. specific map features. programmable Then use 50 measurement calculations to customize operations, speed analysis trans- tools to automatically repetitive tasks and create specialized analysis forms, graphing isolate and analyze the transforms. In addition, the built-in graphing capabilities, FFT selected features. transforms, and capabilities let you plot your data quickly, so easy data export you can easily see your conclusions. to SigmaPlot and SigmaScan Pro's four calibration SigmaStat for options, including raw pixel, 1D And for more in-depth graphing and statistical analysis, all spreadsheet data advanced graph- single point translation, 2-D ing and statistics. linear distance, and 3-D area, are compatible with both SigmaPlot provide multiple ways to quantify and SigmaStat. your measurements.