MORPHOLOGICAL CHARACTERISTICS of the TONGUE of the ROCK CAVY- Kerodon Rupestris WIED, 1820 (Rodentia, Caviidae) CARACTERÍSTICAS
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1174 Original Article MORPHOLOGICAL CHARACTERISTICS OF THE TONGUE OF THE ROCK CAVY- Kerodon rupestris WIED, 1820 (Rodentia, Caviidae) CARACTERÍSTICAS MORFOLÓGICAS DA LÍNGUA EM MOCÓS- Kerodon rupestris WIED, 1820 (Rodentia,Caviidae) Amilton Cesar dos SANTOS 1; Marian Mazeto de ARO 2; Bruno Machado BERTASSOLI 3; Diego Carvalho VIANA 1; Bruno Gomes VASCONCELOS 4; Rose Eli Grassi RICI 5; Moacir Franco de OLIVEIRA 6; Maria Angélica MIGLINO 5; Antônio Chaves de ASSIS-NETO 5 1. Doutorandos, Faculdade de Medicina Veterinária e Zootecnia- FMVZ. Universidade de São Paulo - USP, São Paulo, SP, Brasil. [email protected], [email protected]; 2. Graduanda, Faculdade de Medicina Veterinária e Zootecnia- FMVZ. Universidade de São Paulo - USP, São Paulo, SP, Brasil; 3. Doutorando, Universidade Federal de Minas Gerais- UFMG, Belo Horizonte, MG, Brasil; 4. Professor Doutor, Universidade Federal de Mato Grosso - UFMT, Sinop, MT, Brasil; 5. Professores Doutores, Faculdade de Medicina Veterinária e Zootecnia – FMVZ - USP, São Paulo, SP, Brasil; 6. Professor, Doutor, Universidade Federal Rural do Semiárido - UFERSA, Mossoró, RN, Brasil; ABSTRACT: The Kerodon rupestris are wild rodents bred in captivity in order to their conservation and development of researches. The aim of this study was describe the morphology of the tongue from eight animals by light microscopy and scanning electron microscopy, beyond macroscopic studies. The tongues of K. rupestris had 3.15 ± 0.28 cm of length; 1.0 ± 0.20 cm width at the root; 0.38 ± 0.10 cm width at the body; and, 1.10 ± 0.09 cm width at the apex. Thickness measurements were 0.50 ± 0.09 cm at the apex; 0.30 ± 0.10 cm at the body; and, 0.70 ± 0.10 cm at the root. The free apex had 0.50 ± 0.10 cm in length. The dorsal surface of the tongue contained the fungiform and filiform papillae at the apex and body; conical papillae and a pairs of vallate papillae at the root, and foliated papillae dorsolaterally positioned in the root region. The proper lamina of the dorsal surface of the tongue was composed by keratinized stratified squamous epithelium, in which lingual papillae were found; and, a rich network of skeletal striated muscle and non-modeled dense connective tissue, in which vessels, nerves, mucous and serous acini and their respective ducts were found. In summary, dorsal surface of the tongue of K. rupestris had filiform and conical papillae with mechanical function, and, fungiform, vallate and foliated papillae with taste buds responsible by gustatory function. Moreover, the tongue of these animals showed some peculiarities as the eminence on the apex in sagittal plane, which needs further studies. KEYWORDS: Digestory apparatus. Lingual papillae. Oral cavity. Wild rodents. INTRODUCTION development of researches (OLIVEIRA et al., 2011; SANTOS et al., 2014). The rock cavies Kerodon rupestris are wild In this sense, few studies focusing on the rodents, which have been restricted to the semiarid digestory apparatus of the K. rupestris have been region of Caatinga in the Brazilian Northeastern. published and they described teeth (THOMAZ et They belong to the Caviidae family, Caviinae al., 2006) and stomach (BORSARI et al., 2010) subfamily (ADRIAN; SACHSER, 2011; morphology. Another important organ of digestory RODRIGUES et al., 2013) and Kerodon genus apparatus is the tongue, in which, different types of (THOMAZ et al., 2006). Their habit consist of functional lingual papillae (WITT; REUTTER, stalking in the afternoon or at night to seek leaves, 1997; BRANCO et al., 2011, 2012; buds, twigs, fruits, bark, roots and tubers from the MANÇANARES et al., 2012) lie on the dorsum, shrubs of the Caatinga (OLIVEIRA et al., 2011). and are variously distributed in different vertebrate The Caviidae are prey of most South species (IWASAKI, 2002; JACKOWIAK; American predators and thus they have important GODYNICKI, 2005; NONAKA; ZHENG; ecological role in the food chain. Moreover, the K. KOBAYASHI, 2008). The aim of this study was rupestris are subject to intense hunting due to their describe the morphological characteristics of the size and tasty meat, which is greatly appreciated by tongue of K. rupestris , providing more data for the rural population in the Brazilian Northeastern future researches. (ZOGNO; MIGLINO; OLIVEIRA, 2004). For this reason, K. rupestris and other Brazilian Caviidae are bred in captivity in order to provide alternative source of proteins, preserve the species and to Received: 09/04/14 Biosci. J., Uberlândia, v. 31, n. 4, p. 1174-1182, July/Aug. 2015 Accepted: 15/10/14 1175 Morphological characteristics... SANTOS, A. C. MATERIAL AND METHODS tissues were washed with buffer solution and dehydrated in increasing ethanol series (from 50 to Animals 100%), followed by critical point drying (Balzers Tongues of four males and four females PCD 020). Then, the fragments were properly fixed (adults and healthy) from the Breeding Center in metallic (stubs) and coated in gold (Emitech (CEMAS) of the Federal Rural University of the K550). The analysis was performed with electron Semiarid Region (UFERSA), Mossoró RN Brazil microscope Leo 435 VP (Semtech, USA). (IBAMA 2028236/2008) were used in present study. The study was approved by the Bioethics Light Microscopy Analysis (LM) Committee of the School of Veterinary Medicine From the tongues fixed in 10% and Animal Science, University of São Paulo, São formaldehyde, samples of apex, body, root and Paulo SP Brazil (n o 2485/2011). The animals came lateral regions of the root were extracted. Then, the from other previous studies and they had already fragments were dehydrated in increasing series of been euthanized and fixed in formaldehyde 10% ethanol (from 70 to 100%) and diaphanized in (four animals) and glutaraldehyde 2.5% 0.1M (four xylene for inclusion in paraffin blocks. Cuts with animals). 5µm in Leica RM 2155 microtome were obtained and stained with hematoxylin and eosin (HE) in Macroscopic Analysis order to analyze the microscopic morphology of the The tongues were dissected, removed and tongue. BX61VS Olympus photomicroscope was then, total length, width and thickness at the apex, used for microscopic photodocumentation. body and root were obtained with digital calipers (Mitotuyo, Tokyo, Japan). So, a schematic drawing RESULTS of the morphology of the tongue for a better view of your shape was performed. The measurements of Macroscopic analysis mean and standard deviation were performed. The tongues of K. rupestris had 3.15 ± 0.28 Photodocumentation was performed by camera cm of length; 1.0 ± 0.20 cm width at the root; 0.38 ± Olympus SP 810UZ 14 mp. 0.10 cm width at the body; and, 1.10 ± 0.09 cm width at the apex. Thickness measurements were Scanning Electron Microscopy Analysis (SEM) 0.50 ± 0.09 cm at the apex; 0.30 ± 0.10 cm at the The tongues fixed in glutaraldehyde 2.5% body; and, 0.70 ± 0.10 cm at the root. The free apex 0.1M (pH 7.4) were sectioned into three parts: root, had 0.5 ± 0.10 cm of length (Figure 1A and B). body and apex. Then, the fragments were washed Morphologically, an intermolar eminence at the root with 0.1 M phosphate buffer solution and post-fixed and a lower eminence in the sagittal plane at the in osmium tetroxide (Spurr's Kit-Electron apex were noted (Figure 1A and B; 2A). Microscopy Sciences Co., USA). The fragments of Figure 1. Illustration of the anatomical shape and measurements of the tongue of K. rupestris . A: shape and measurements of length and width at the apex (a), body (b), and root (r). B: shape and measurements of length and thickness at the apex (a), body (b), and root (r). Biosci. J., Uberlândia, v. 31, n. 4, p. 1174-1182, July/Aug. 2015 1176 Morphological characteristics... SANTOS, A. C. Scanning Electron Microscopy Analysis (SEM) was scattered among the filiform papillae at the By SEM, we observed that the dorsum of body and apex (Figure 2D). A pair of vallate the tongue had several filiform papillae scattered papillae with rectilinear grooves at the root was throughout the body and apex (Figure 2D); conical present (Figure 2B). Foliate papillae at the root was papillae was scattered in the region of the intermolar dorsum-laterally positioned (Figure 2C). eminence at root (Figure 2B); fungiform papillae Figure 2. Photography and electron micrograph of the tongue of K. rupestris . A: Photograph showing root (r), body (b), and apex (a) and median eminence (*) at the apex. B: electron micrograph showing vallate (circle) and conical papillae (arrow) at the root. Bar: 1 mm. C: electron micrograph from lateral view of root showing foliated papillae (arrow) and vallate papillae (arrowhead). Bar: 1 mm. D: electron micrograph showing foliated papillae at the body. Bar: 100 µm. E: electron micrograph at the body showing fungiform papillae (f) surrounded by filiform papillae. Bar: 100 µm. Biosci. J., Uberlândia, v. 31, n. 4, p. 1174-1182, July/Aug. 2015 1177 Morphological characteristics... SANTOS, A. C. Light Microscopy Analysis (LM) squamous epithelium in the ventral surface of The proper lamina of the dorsal surface of tongue was found (Figure 3C and D). the tongue was composed by the surface covered Unilocular adipose tissue was found with keratinized stratified squamous epithelium, in between muscular bundles (Figure 3D). The ventral which several papillae were found and non-modeled surface of the tongue had keratinized stratified dense connective tissue, in which vessels, nerves, squamous epithelium and absence of lingual mucous and serous acini and their respective ducts papillae (Figure 3C). were found (Figure 3A, B, E and F; Figure 4A and The fungiform (Figure 3B), foliated (Figure B). Moreover, a rich network of skeletal striated 4B and C) and vallate papillae (Fig. 3E) had taste muscle, with longitudinal, transversal and circular buds (Figure 3E). In the region of the vallate and fibers over its entire length and keratinized stratified foliated papillae, serous and mucous acini were found (Figure 3E and F; 4B).