The Michoacán Centipede Snake, Tantilla Cascadae Wilson

Herpetology Notes, volume 14: 263-268 (2021) (published online on 01 February 2021)

The Michoacán Centipede Snake, Tantilla cascadae Wilson and Meyer, 1981 (Squamata: Colubridae): New record for Jalisco, with notes on conservation, biogeography, and a key to the species of the genus in western Mexico

Aldo Dávalos-Martínez1,*, Víctor Fernando Záizar-Gutiérrez1, Daniel Cruz-Sáenz2, Eduardo Daniel Roldán- Olvera1, and Larry David Wilson3

Abstract. Tantilla cascadae is a rare centipede snake that inhabits western Mexico and is only known from two localities and three specimens. In this work we report two new specimens from a new locality in the municipality of Tamazula de Gordiano, Jalisco, Mexico. We assess the biogeographical and physiographical component of the species’ distribution, and discuss the conservation status of the species proposing a new IUCN conservation assessment of Endangered. We additionally present a dichotomous key for the genus Tantilla in western Mexico.

Keywords. Southern Jalisco, western Michoacán, IUCN, land-use change, mining, avocado, new locality

Introduction This species is a member of the Tantilla calamarina species group, which consists of seven species The Michoacán Centipede Snake, Tantilla cascadae distributed along the Pacific versant from northwestern Wilson and Meyer, 1981, is a centipede snake of the Mexico to Costa Rica (Canseco-Márquez et al., 2007b): family Colubridae, endemic to western Mexico. It Tantilla calamarina Cope, 1866; T. cascadae Wilson inhabits oak and pine-oak forest (Duellman, 1961; Cruz- and Meyer, 1981; T. ceboruca Canseco-Márquez, Sáenz et al., 2015) in south-central Jalisco and central Smith, Ponce-Campos, Flores-Villela and Campbell, Michoacán at elevations of 1430–1858 m (Wilson and 2007b; T. coronadoi Hartweg, 1944; T. deppei (Bocourt, Mata-Silva, 2015). The species is classified by the 1883); T. sertula Wilson and Campbell, 2000; and T. Mexican law as Threatened (SEMARNAT, 2010), as vermiformis (Hallowell, 1861) (Wilson and Mata-Silva Data Deficient (DD) by the IUCN Red List (Canseco- 2015). Wilson and Meyer (1981) defined the group by Márquez et al., 2007a), and has an Environmental the presence of a middorsal dark stripe and a dark lateral Vulnerability Score (EVS) of 16 (Johnson et al., 2017). stripe on rows three and four, except in T. deppei, which can have two stripes (one on rows two and three and another in row five) and in T. cascadae, in which the

1 Universidad de Guadalajara, Centro Universitario de Ciencias lateral stripe on rows three and four disappears on the Biológicas y Agropecuarias, km 15.5 Carretera Guadalajara- forepart of the body. Some species of the group also Nogales, Predio Las Agujas, A.P. 1-1919, Zapopan 44101, have the central portion of the parietals covered with a Jalisco, México. spatulate anterior extension of the middorsal dark stripe, 2 Universidad de Guadalajara, Centro Universitario de Ciencias which continues anteriorly to cover the remainder of Biológicas y Agropecuarias, Centro de Estudios en Zoología, the head. On either side of this central head mark a km 15.5 Carretera Guadalajara-Nogales, Predio Las Agujas, postparietal pale spot usually grades posteriorly into A.P. 1-1919, Zapopan 44101, Jalisco, México. 3 Centro Zamorano de Biodiversidad, Escuela Agrícola the ground colour of the dorsolateral area between the Panamericana Zamorano, Departamento de Francisco lateral and median dark stripes and connects anteriorly Morazán, Honduras; 1350 Pelican Court, Homestead, Florida with a narrow extension that proceeds along the side of 33035-1031, USA. the parietal, over the supraocular and onto the side of the * Corresponding author. E-mail: prefrontals and internasals. [email protected] Virtually nothing is known on the natural history of © 2021 by Herpetology Notes. Open Access by CC BY-NC-ND 4.0. Tantilla cascadae except for the note on the specimen 264 Aldo Dávalos-Martínez �� found in 2000 by Heimes (2016) at the type locality: Comisión Nacional de Áreas Naturales Protegidas “after being exposed from its hiding-place under a small (CONANP). rock the snake tried to retreat quickly into a deeper For the dichotomous key, we defined “western Mexico” subterranean passage”. as the region including the states of Aguascalientes, Tantilla cascadae is a rare species only known from Colima, Jalisco, Michoacán, Nayarit, and Zacatecas. three records: the holotype specimen (AMNH 107389) We obtained morphological and geographic distribution from “Tzaráracua Falls (=Cascada la Tzaráracua), S of data from Reyes-Velasco et al. (2012), Wilson and Uruapán [10.5 km south, according to Duellman, 1961], Mata-Silva (2014) and Heimes (2016). Michoacán, México.” (Wilson and Meyer, 1981), one photographed individual collected by Heimes (2016) Results also from the type locality, and one individual collected New records. Herein we present two new specimens in the municipality of Pihuamo, ca. 20 km southeast of Tantilla cascadae from a new locality in the from the urban area of Pihuamo, Jalisco, Mexico municipality of Tamazula de Gordiano, Jalisco, Mexico (CEZUG-R304; Cruz-Sáenz et al., 2015). (Fig. 1). On 1 July 2019 in Cerro La Mesa, 1.14 km NW In this work we assess the species’ conservation status, of Tamazula de Gordiano (19.6849°N, -103.2572°W; its biogeographic and physiographic regionalisation, WGS 84; elevation 1447 m) we found a female T. and provide to its distribution a new locality with two cascadae (CEZUG-R436); the snake measured 188.03 new specimens. We also provide a dichotomous key for mm in total length with a tail/total length ratio of 0.198. the species of Tantilla in western Mexico. The specimen has 145 ventrals, 42 subcaudals, six supralabials, six infralabials, two postoculars, and a Materials and Methods lateral dark stripe that is barely perceptible on the first third of the body, but is more noticeable towards the We undertook diurnal fieldwork in the municipality posterior end of the body. We kept the snake in captivity of Tamazula de Gordiano in Jalisco, Mexico. We for a few months before euthanising it and we feed it collected the specimens under collection permit SGPA/ with larvae of the mealworm beetle (Tenebrio molitor DGVS/01205/17, granted to Fausto R. Méndez de la Linnaeus, 1758). To our knowledge, this is the first data Cruz by the Secretaria de Medio Ambiente y Recursos on the diet in captivity, and in general, for the species. Naturales. We confirmed the identification of the new On 14 March 2020 in Cerro La Mesa, 1.7 km N of specimens reported (CEZUG-R436-R445) using the Tamazula de Gordiano (19.6907°N, -103.2534°W; keys and descriptions provided by Wilson and Mata- WGS 84; elevation 1358 m), we found another female Silva (2014). Tantilla cascadae (CEZUG-R445). The snake measured We discuss the species’ conservation assessment 192.6 mm in total length with a tail/total length ratio of based on observations in the field made at the three 0.175. The specimen has 141 ventrals, 38 subcaudals, six known localities. We assess the Extent of Occurrence supralabials, six-five infralabials, and two postoculars. (EOO) and Area of Occupancy (AOO) with Geospatial We could not obtain data on the lateral stripe of this Conservation Assessment Tool (GeoCAT; Bachman et specimen. al., 2011) using the auto-value cell width of 13 km and We found both specimens (vouchers CEZUG-R436 we made the IUCN categorisations using the Guidelines and CEZUG-R445) under rocks while conducting for Using the IUCN Red List Categories and Criteria diurnal fieldwork. The habitat of the site is tropical (IUCN Standards and Petitions Committee, 2019). deciduous forest. We deposited the specimens in the We obtained biogeographical data with QGIS vertebrate collection of the Centro de Estudios en Geographic Information System and Google Earth. Zoología of the Universidad de Guadalajara. For assessing the physiographic and biogeographic These records represent third and fourth vouchered provinces of the species, we used the regionalisation specimens of T. cascadae, the second record for the as proposed by Cruz-Sáenz et al. (2017), INEGI state of Jalisco, and the third known locality for the (INEGI, 2020; Biblioteca digital de Mapas. Available species (Fig. 2). These records extend its known from: https://www.inegi.org.mx/app/biblioteca/ficha. distribution 62.3 km north from the Pihuamo locality html?upc=702825267575 [Accessed 20 May 2020)]) (CEZUG-R304) and 129 km northwest from the type and Morrone et al. (2016). We obtained the Natural locality (AMNH 107389). The new locality represents protected areas data from the shape files provided by the lowest elevation recorded for the species (1358 m The Michoacán Centipede Snake: New record for Jalisco, western Mexico 265

Figure 1. (A) Tantilla cascadae (CEZUG R-436) in life. (B) Tantilla cascadae (CEZUG R 445) in life and (C) after euthanasia. Photographs by Eduardo D. Roldán-Olvera (A), Víctor F. Záizar-Gutiérrez (B) and Aldo Dávalos-Martínez (C).

for CEZUG-R445), the northernmost locality, and the local tourist activities that take place there, including first record in tropical deciduous forest. littering and the use of horses for recreational purposes. Conservation. Tantilla cascadae is only known from three localities. We estimated an EOO of 2977.385 km2 and an AOO of 500.460 km2. The EOO of the species lies in southeastern Jalisco and western Michoacán. This is one of the areas with the highest rate of illegal land-use change in western Mexico, principally due to the recent and current increase of avocado plantings. Although all the EOO is at risk, each locality has its own environmental problems. Uruapan and its surroundings are attacked constantly by human caused-forest fires and illegal logging for land-use change, whether for developing new urban areas or for preparing new sites for avocado tree planting. The pesticides and herbicides used in agricultural activities also cause environmental damage in the area. The type locality, the Tzaráracua Falls, is at constant risk in due to the contamination of Figure 2. Known distribution of Tantilla cascadae in western the Cupatitzio River, the presence of loose livestock, and Mexico. Map by Víctor F. Záizar-Gutiérrez. 266 Aldo Dávalos-Martínez ��

TableTable 1. 1.Physiographic Physiographic and and biogeographicbiogeographic regionalization regionalisation of ofTantilla Tantilla cascadae cascadae. .

Voucher Mexican biogeographic provinces INEGI Physiographic INEGI Physiographic Physiographic regions of Jalisco (Morrone et al., 2016) Provinces Subprovinces (Cruz-Sáenz et al., 2017) AMNH 107389 Transmexican Volcanic Belt Eje Neovolcánico Escarpa Limítrofe del Sur No data available CEZUG-R304 Sierra Madre del Sur Sierra Madre del Sur Cordillera Costera del Sur Sierra de Coalcomán CEZUG-R436 Pacific Lowlands Sierra Madre del Sur Cordillera Costera del Sur Trans-Mexican Volcanic Belt CEZUG-R445 Pacific Lowlands Sierra Madre del Sur Cordillera Costera del Sur Trans-Mexican Volcanic Belt

Also, the municipal garbage dump of Uruapan is nearby, Recursos Naturales Las Huertas (60 km from both potentially causing contamination and presence of rats, localities). feral cats, and feral dogs in the area. When Tantilla cascadae was first assessed in terms of The new locality reported here for CEZUG-R436 the IUCN Red List (Canseco-Márquez et al., 2007a) the and CEZUG-R445, although very close to the town species was only known from the type locality; since of Tamazula de Gordiano and with loose livestock and then, two new localities have been reported (including local tourist activities, remains relatively well conserved the one herein presented) providing new information because the people of the town see it as a natural reserve. regarding its known distribution. Due to the EOO and A few little opencast mines were started many years AOO results, the potential of habitat loss potential to ago and now are abandoned. The surroundings of the the southeastern Jalisco and eastern Michoacán area, town, however, are highly at risk due to the agricultural and the conservation problems as discussed above, we activities of the planting of sugar cane and berries, recommend a conservation assessment of Endangered which includes herbicide and pesticide contamination, (EN) [B1a (i, ii, iii)] for this species. water loss, and land-use change. Biogeography. We followed four physiographic and biogeographic regionalisation proposals to describe the The locality reported in Pihuamo for CEZUG-R304 1 is the most threatened one. Until recently the primary biogeographic component of the distribution of Tantilla vegetation of the area was well conserved, mainly due cascadae (Table 1), only the type locality could not be to drug related activities in the site. Although it has assessed with the proposal made by Cruz-Sáenz et al. been lately at a high risk for the increasing opencast (2017). With the three known localities of the species, 2 mining in the area, where at least two big mines, one we estimated an EOO of 2977.385 km . of manganese and one of iron, have been operating for Dichotomous key. Six species of Tantilla occur in the last several years. As is known, opencast mining is a western Mexico; Tantilla bocourti (Günther, 1895) very invasive activity and the habitat at that site is close (T. rubra species group), T. calamarina, T. cascadae, potentially to being lost. T. ceboruca (T. calamarina species group), T. wilcoxi Another conservation concern is that none of the Stejneger, 1902 and T. yaquia Smith, 1942 (T. planiceps localities are within any natural protected areas (NPA). species group) (Wilson and Mata-Silva, 2014). Natural protected areas are listed in order of nearest to Dichotomous key of the species of the genus Tantilla farthest and with its approximately distance from each from western Mexico: locality. The Uruapan locality (AMNH 107389) has 1 Dorsum uniform in colour or nearly so, without three NPAs around it, the Parque Nacional Barranca stripes or bands … 2 del Cupatitzio (9 km), Área de Protección de Flora y - Dorsum with definite stripes or bands … 5 Fauna Pico de Tancítaro (20 km), and Reserva de la 2 Pale nuchal band crosses posterior portion of Biósfera Zicuirán-Infiernillo (45 km). Both the Pihuamo parietals … 3 (CEZUG R-304) and the Tamazula de Gordiano - Pale nuchal band borders parietals or present 1–3 localities (CEZUG R-436 and CEZUG R-445) have scales posterior to parietals … 4 the same three NPAs around them, the Parque Nacional Nevado de Colima (40 km from Tamazula de Gordiano 3 Secondary temporal elongate; ventrals 135–164; and 60 km from Pihuamo), Área de Protección de Flora black head cap extends to or below corner y Fauna El Jabalí (50 km from Tamazula de Gordiano of mouth, including parts of 6th and 7th and 60 km from Pihuamo) and Área de Protección de infralabials. In the study area, this species is The Michoacán Centipede Snake: New record for Jalisco, western Mexico 267

present in Aguascalientes, northern Jalisco, and biogeographical patterns within the Tantilla calamarina southern Zacatecas … T. wilcoxi species group. - Secondary temporal about same size as dorsal Conservation of narrowly-distributed endemic body scales; ventrals 160–195; black head species is of vital importance, especially in areas with cap does not extend to corner of mouth, or high degrees of environmental impact. With the data below it onto 6th and 7th infralabials. The presented herein, we support the importance of tropical species is widely distributed in western Mexico and temperate forests from southern Jalisco and western … T. bocourti (part) Michoacán for the conservation of Tantilla cascadae and as a reservoir of endemic taxa. 4 Pale nuchal band distinct, bordered behind by Further research and conservation actions should dark nape band; ventrals 160–195; varying pale be taken in place to help the conservation of Tantilla markings usually present on the internasals cascadae, such as the discovery of new localities, and and prefrontals and sometimes on the anterior in-situ conservation programs in the known distribution portion of the supraoculars and parietals. This areas. species is widely distributed in western Mexico … T. bocourti (part) Acknowledgments. We thank Dante S. Figueroa-Martínez, - Pale nuchal band sometimes distinct, not Sabina Ascencio-Ramírez, Oassis F. Huerta-Galván, and Jairo D. bordered posteriorly by dark pigment, if so, Uribe-Mendoza for providing valuable comments and information pigment reduced to series of spots; ventrals that improved the manuscript’s first draft. We also thank Fausto 134–165; a pale preocular spot is present on the R. Méndez de la Cruz for providing the collection permit SGPA/ 1st supralabial, and a prominent pale postocular DGVS/01205/17 issued by the Secretaría de Medio Ambiente y spot on the lower 1/3 – ½ of the anterior Recursos Naturales. temporal and the majority of the 4th, 5th, and 6th supralabials. In the study area the species is References present in northern Nayarit … T. yaquia Bachman, S., Moat, J., Hill, A.W., de la Torre, J., Scott, B. (2011): Supporting Red List threat assessments with GeoCAT: 5 A single postocular; ventrals 106–140. In the geospatial conservation assessment tool. In e-Infrastructures study area is present on the pacific versant for data publishing in biodiversity science. Smith, V. and L. of Nayarit, Jalisco, Colima, and Michoacán Penev (Eds.). ZooKeys 150: 117–126. https://doi.org/10.3897/ … T. calamarina zookeys.150.2109. - Two postoculars; ventrals 136–153 … 6 Bocourt, M.F. (1883): Etudes sur les reptiles. Pp. 1–1012 In Recherches Zoologiques pour servir a l’Histoire de Ia Faune 6 Supralabials 6. This species is present in south- de l’Amérique Centrale et du Mexique. Mission Scientifique eastern Jalisco and west-central Michoacán au Mexique et dans l’Amér Imprimerie Impériale. Duméril, A., … T. cascadae M.F. Bocourt, and F. Mocquard. Paris, France. - Supralabials 7 This species is known only Canseco-Márquez, L., Campbell, J.A., Ponce-Campos, P., from three localities in south-central Nayarit, Muñoz-Alonso, A., García-Aguayo, A. (2007a): Tantilla cascadae. The IUCN Red List of Threatened Species 2007: west-central Jalisco, and northern Colima e.T63939A12730009. Accessed on 28 May 2020. https://dx.doi. … T. ceboruca org/10.2305/IUCN.UK.2007.RLTS.T63939A12730009.en. Canseco-Márquez, L., Smith, E.N., Ponce-Campos, P., Flores- Discussion Villela, O., Campbell, J.A. (2007b): A new species of Tantilla (Squamata: Colubridae) of the calamarina group from Volcán Regional dichotomous keys can be of valuable help Ceboruco, Nayarit, Mexico. Journal of Herpetology 41: when dealing with genera with high numbers of species, 220–224. https://doi.org/10.1670/0022-1511(2007)41[220: such as Tantilla. Our key can be a tool for students, ANSOTS]2.0.CO;2. naturalists, and environmental consultants that sample Cope, E.D. (1866): Fifth contribution to the herpetology of tropical and conduct research in western Mexico and can help America. Proceedings of the Academy of Natural Sciences of them to identify correctly this and other species, which Philadelphia 18: 317–323. can result in the generation of more knowledge about Cruz-Sáenz, D., Muñoz-Nolasco, F.J., Lazcano, D., Flores- Covarrubias, E. (2015): Noteworthy records for Tantilla cascadae this still poorly-known species. and T. ceboruca (Squamata: Colubridae) from Jalisco, Mexico. The biogeographical and physiographical data Check List 11: 1–5. http://dx.doi.org/10.15560/11.4.1708. provided in this work, in addition to molecular data, can Cruz-Sáenz, D., Muñoz-Nolasco, F.J., Mata-Silva, V., Johnson, be used in the future to hypothesise the evolutionary and J.D., García-Padilla, E., Wilson, L.D. (2017): The herpetofauna 268 Aldo Dávalos-Martínez ��

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Accepted by Robson Ávila