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FOR IN VITRO RESEARCH USE ONLY Europe: [email protected] NOT FOR USE IN HUMANS OR ANIMALS China: [email protected] MAD2L2 Polyclonal An body Catalog number: 12683-1-AP Background Size: 27 μg/150 μl MAD family, together with BUB and Mps1,Cdc20k, play roles in the mito c spindle Source: Rabbit checkpoint. MAD2L2 is one of the MAD family. It can mediates the second Isotype: IgG polymerase switching in transla on DNA synthesis by media ng the inteac on Synonyms: between the error-prone DNA polymerase zeta cataly c subunit REV3L and the MAD2L2; hREV7, MAD2 like inserter polymerase REV1. Through regula on of the JNK-mediated protein 2, MAD2B, MAD2L2, phosphoryla on and ac va on of the transcrip onal ac vator ELK1, MAD2L2 REV7, REV7 homolog involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferen a on.
Applications Tested applica ons: ELISA, WB, IP Cited applica ons: WB Species specificity: Human,Mouse,Rat; other species not tested. Cited species: Human human brain ssue were subjected to Caculated MAD2L2 MW: 211aa,24 kDa SDS PAGE followed by western blot with 12683-1-AP(MAD2L2 an body) Observed MAD2L2 MW: 24kd at dilu on of 1:500 Posi ve WB detected in Human brain ssue, A375 cells, fetal human brain ssue, HEK-293 cells, HeLa cells, human colon ssue, human kidney ssue, K-562 cells, mouse brain ssue Posi ve IP detected in Mouse brain ssue Recommended dilu on: WB: 1:500-1:5000 IP: 1:200-1:2000 Applica on key: WB = Western blo ng, IHC = Immunohistochemistry, IF = Immunofluorescence, IP = Immunoprecipita on
IP Result of an -MAD2L2 (IP:12683- Immunogen information 1-AP, 3ug; Detec on:12683-1-AP 1:500) with mouse brain ssue lysate Immunogen: Ag3373 3600ug. GenBank accession number: BC015244 Gene ID (NCBI): 10459 Full name: MAD2 mito c arrest deficient-like 2 (yeast)
Product information Purifica on method: An gen affinity purifica on Storage: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.