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DATASHEET USA: [email protected] FOR IN VITRO RESEARCH USE ONLY Europe: [email protected] NOT FOR USE IN HUMANS OR ANIMALS China: [email protected] MAD2L2 Polyclonal Anbody Catalog number: 12683-1-AP Background Size: 27 μg/150 μl MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitoc spindle Source: Rabbit checkpoint. MAD2L2 is one of the MAD family. It can mediates the second Isotype: IgG polymerase switching in translaon DNA synthesis by mediang the inteacon Synonyms: between the error-prone DNA polymerase zeta catalyc subunit REV3L and the MAD2L2; hREV7, MAD2 like inserter polymerase REV1. Through regulaon of the JNK-mediated protein 2, MAD2B, MAD2L2, phosphorylaon and acvaon of the transcriponal acvator ELK1, MAD2L2 REV7, REV7 homolog involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferenaon. Applications Tested applicaons: ELISA, WB, IP Cited applicaons: WB Species specificity: Human,Mouse,Rat; other species not tested. Cited species: Human human brain ssue were subjected to Caculated MAD2L2 MW: 211aa,24 kDa SDS PAGE followed by western blot with 12683-1-AP(MAD2L2 anbody) Observed MAD2L2 MW: 24kd at diluon of 1:500 Posive WB detected in Human brain ssue, A375 cells, fetal human brain ssue, HEK-293 cells, HeLa cells, human colon ssue, human kidney ssue, K-562 cells, mouse brain ssue Posive IP detected in Mouse brain ssue Recommended diluon: WB: 1:500-1:5000 IP: 1:200-1:2000 Applicaon key: WB = Western blong, IHC = Immunohistochemistry, IF = Immunofluorescence, IP = Immunoprecipitaon IP Result of an-MAD2L2 (IP:12683- Immunogen information 1-AP, 3ug; Detecon:12683-1-AP 1:500) with mouse brain ssue lysate Immunogen: Ag3373 3600ug. GenBank accession number: BC015244 Gene ID (NCBI): 10459 Full name: MAD2 mitoc arrest deficient-like 2 (yeast) Product information Purificaon method: Angen affinity purificaon Storage: PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC..

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Identification of Conserved Genes Triggering Puberty in European Sea
Blázquez et al. BMC Genomics (2017) 18:441 DOI 10.1186/s12864-017-3823-2 RESEARCHARTICLE Open Access Identification of conserved genes triggering puberty in European sea bass males (Dicentrarchus labrax) by microarray expression profiling Mercedes Blázquez1,2* , Paula Medina1,2,3, Berta Crespo1,4, Ana Gómez1 and Silvia Zanuy1* Abstract Background: Spermatogenesisisacomplexprocesscharacterized by the activation and/or repression of a number of genes in a spatio-temporal manner. Pubertal development in males starts with the onset of the first spermatogenesis and implies the division of primary spermatogonia and their subsequent entry into meiosis. This study is aimed at the characterization of genes involved in the onset of puberty in European sea bass, and constitutes the first transcriptomic approach focused on meiosis in this species. Results: European sea bass testes collected at the onset of puberty (first successful reproduction) were grouped in stage I (resting stage), and stage II (proliferative stage). Transition from stage I to stage II was marked by an increase of 11ketotestosterone (11KT), the main fish androgen, whereas the transcriptomic study resulted in 315 genes differentially expressed between the two stages. The onset of puberty induced 1) an up-regulation of genes involved in cell proliferation, cell cycle and meiosis progression, 2) changes in genes related with reproduction and growth, and 3) a down-regulation of genes included in the retinoic acid (RA) signalling pathway. The analysis of GO-terms and biological pathways showed that cell cycle, cell division, cellular metabolic processes, and reproduction were affected, consistent with the early events that occur during the onset of puberty.
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Human Pol Ζ Purified with Accessory Subunits Is Active in Translesion DNA Synthesis and Complements Pol Η in Cisplatin Bypass
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Gene Section Review
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Anti-MAD2L2 Monoclonal Antibody, Clone FQS24768 (DCABH-6886) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use
Anti-MAD2L2 monoclonal antibody, clone FQS24768 (DCABH-6886) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview Rabbit Monoclonal to Mad2L2 Antigen Description Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. Immunogen Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Mad2L2 aa 150 to the C-terminus. The exact sequence is proprietary.Database link: Q9UI95 Isotype IgG Source/Host Rabbit Species Reactivity Mouse, Rat, Human Clone FQS24768 Purity Protein A purified Conjugate Unconjugated Applications IP, ICC/IF, Flow Cyt, IHC-P, WB Positive Control K562, SW480, Hela, Molt-4 cell lysates, human ovarian carcinoma tissue, Hela cells, Format Liquid Size 100 μl Buffer Preservative: 0.01% Sodium azide; Constituents: 59% PBS, 0.05% BSA, 40% Glycerol 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Preservative 0.01% Sodium Azide Storage Store at +4°C short term (1-2 weeks).
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MAD2L2 Monoclonal ANTIBODY
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Cornelia De Lange Syndrome-Associated Mutations Cause a DNA Damage Signalling and Repair Defect
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MAD2 (MAD2L1) Mouse Monoclonal Antibody [Clone ID: 17D10] Product Data
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