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Cervid TB DPP Testing August 2017
Cervid TB DPP Testing August 2017 Elisabeth Patton, DVM, PhD, Diplomate ACVIM Veterinary Program Manager Wisconsin Department of Agriculture, Trade and Consumer Protection Division of Animal Health Why Use the Serologic Test? Employ newer, accurate diagnostic test technology Minimizes capture and handling events for animal safety Expected to promote additional cervid TB testing • Requested by USAHA and cervid industry Comparable sensitivity and specificity to skin tests 2 Historical Timeline 3 Stat-Pak licensed for elk and red deer, 2009 White-tailed and fallow deer, 2010-11 2010 - USAHA resolution - USDA evaluate Stat-Pak as official TB test 2011 – Project to evaluate TB serologic tests in cervids (Cervid Serology Project); USAHA resolution to approve Oct 2012 – USDA licenses the Dual-Path Platform (DPP) secondary test for elk, red deer, white-tailed deer, and fallow deer Improved specificity compared to Stat-Pak Oct 2012 – USDA approves the Stat-Pak (primary) and DPP (secondary) as official bovine TB tests in elk, red deer, white- tailed deer, fallow deer and reindeer Recent Actions Stat-Pak is no longer in production 9 CFR 77.20 has been amended to approve the DPP as official TB program test. An interim rule was published on 9 January 2013 USDA APHIS created a Guidance Document (6701.2) to provide instructions for using the tests https://www.aphis.usda.gov/animal_health/animal_ diseases/tuberculosis/downloads/vs_guidance_670 1.2_dpp_testing.pdf 4 Cervid Serology Project Objective 5 Evaluate TB detection tests for official bovine tuberculosis (TB) program use in captive and free- ranging cervids North American elk (Cervus canadensis) White-tailed deer (Odocoileus virginianus) Reindeer (Rangifer tarandus) Primary/screening test AND Secondary Test: Dual Path Platform (DPP) Rapid immunochromatographic lateral-flow serology test Detect antibodies to M. -
Phylogenetic Analysis of Eight Sudanese Camel Contagious Ecthyma Viruses Based on B2L Gene Sequence Abdelmalik I
Khalafalla et al. Virology Journal (2015) 12:124 DOI 10.1186/s12985-015-0348-7 RESEARCH Open Access Phylogenetic analysis of eight sudanese camel contagious ecthyma viruses based on B2L gene sequence Abdelmalik I. Khalafalla1,2* , Ibrahim M. El-Sabagh3,4, Khalid A. Al-Busada5, Abdullah I. Al-Mubarak6 and Yahia H. Ali7 Abstract Background: Camel contagious ecthyma (CCE) is an important viral disease of camelids caused by a poxvirus of the genus parapoxvirus (PPV) of the family Poxviridae. The disease has been reported in west and east of the Sudan causing economical losses. However, the PPVs that cause the disease in camels of the Sudan have not yet subjected to genetic characterization. At present, the PPV that cause CCE cannot be properly classified because only few isolates that have been genetically analyzed. Methods and results: PCR was used to amplify the B2L gene of the PPV directly from clinical specimens collected from dromedary camels affected with contagious ecthyma in the Sudan between 1993 and 2013. PCR products were sequenced and subjected to genetic analysis. The results provided evidence for close relationships and genetic variation of the camel PPV (CPPV) represented by the circulation of both Pseudocowpox virus (PCPV) and Orf virus (ORFV) strains among dromedary camels in the Sudan. Based on the B2L gene sequence the available CPPV isolates can be divided into two genetic clades or lineages; the Asian lineage represented by isolates from Saudi Arabia, Bahrain and India and the African lineage comprising isolates from the Sudan. Conclusion: The camel parapoxvirus is genetically diverse involving predominantly viruses close to PCPV in addition to ORFVs, and can be divided into two genetically distant lineages. -
Demographic Observations of Mountain Nyala Tragelaphus Buxtoni in A
y & E sit nd er a v n i g d e Evangelista et al., J Biodivers Endanger Species 2015, 3:1 o i r e Journal of B d f S o p l e a c ISSN:n 2332-2543 1 .1000145 r i DOI: 0.4172/2332-2543 e u s o J Biodiversity & Endangered Species Research article Open Access Demographic Observations of Mountain Nyala Tragelaphus Buxtoni in a Controlled Hunting Area, Ethiopia Paul Evangelista1*, Nicholas Young1, David Swift1 and Asrat Wolde1 Natural Resource Ecology Laboratory Colorado State University B254 Fort Collins, Colorado *Corresponding author: Paul Evangelista, Natural Resource Ecology Laboratory Colorado State University B254 Fort Collins, Colorado, Tel: (970) 491-2302; E-mail: [email protected] Received date: December 4, 2014; Accepted date: January 30, 2015; Published date: February 6, 2015 Copyright: © 2015 Evangelista P. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract The highlands of Ethiopia are inhabited by the culturally and economically significant mountain nyala Tragelaphus buxtoni, an endemic spiral horned antelope. The natural range of this species has become highly fragmented with increasing anthropogenic pressures; driving land conversion in areas previously considered critical mountain nyala habitat. Therefore, baseline demographic data on this species throughout its existing range are needed. Previous studies on mountain nyala demographics have primarily focused on a confined portion of its known range where trophy hunting is not practiced. Our objectives were to estimate group size, proportion of females, age class proportions, and calf and juvenile productivity for a sub-population of mountain nyala where trophy hunting is permitted and compare our results to recent and historical observations. -
Population, Distribution and Conservation Status of Sitatunga (Tragelaphus Spekei) (Sclater) in Selected Wetlands in Uganda
POPULATION, DISTRIBUTION AND CONSERVATION STATUS OF SITATUNGA (TRAGELAPHUS SPEKEI) (SCLATER) IN SELECTED WETLANDS IN UGANDA Biological -Life history Biological -Ecologicl… Protection -Regulation of… 5 Biological -Dispersal Protection -Effectiveness… 4 Biological -Human tolerance Protection -proportion… 3 Status -National Distribtuion Incentive - habitat… 2 Status -National Abundance Incentive - species… 1 Status -National… Incentive - Effect of harvest 0 Status -National… Monitoring - confidence in… Status -National Major… Monitoring - methods used… Harvest Management -… Control -Confidence in… Harvest Management -… Control - Open access… Harvest Management -… Control of Harvest-in… Harvest Management -Aim… Control of Harvest-in… Harvest Management -… Control of Harvest-in… Tragelaphus spekii (sitatunga) NonSubmitted Detrimental to Findings (NDF) Research and Monitoring Unit Uganda Wildlife Authority (UWA) Plot 7 Kira Road Kamwokya, P.O. Box 3530 Kampala Uganda Email/Web - [email protected]/ www.ugandawildlife.org Prepared By Dr. Edward Andama (PhD) Lead consultant Busitema University, P. O. Box 236, Tororo Uganda Telephone: 0772464279 or 0704281806 E-mail: [email protected] [email protected], [email protected] Final Report i January 2019 Contents ACRONYMS, ABBREVIATIONS, AND GLOSSARY .......................................................... vii EXECUTIVE SUMMARY ....................................................................................................... viii 1.1Background ........................................................................................................................... -
Damaliscus Pygargus Phillipsi – Blesbok
Damaliscus pygargus phillipsi – Blesbok colour pattern (Fabricius et al. 1989). Hybridisation between these taxa threatens the genetic integrity of both subspecies (Skinner & Chimimba 2005). Assessment Rationale Listed as Least Concern, as Blesbok are abundant on both formally and privately protected land. We estimate a minimum mature population size of 54,426 individuals (using a 70% mature population structure) across 678 protected areas and wildlife ranches (counts between 2010 and 2016). There are at least an estimated 17,235 animals (counts between 2013 and 2016) on formally Emmanuel Do Linh San protected areas across the country, with the largest subpopulation occurring on Golden Gate Highlands National Park. The population has increased significantly Regional Red List status (2016) Least Concern over three generations (1990–2015) in formally protected National Red List status (2004) Least Concern areas across its range and is similarly suspected to have increased on private lands. Apart from hybridisation with Reasons for change No change Bontebok, there are currently no major threats to its long- Global Red List status (2008) Least Concern term survival. Approximately 69% of Blesbok can be considered genetically pure (A. van Wyk & D. Dalton TOPS listing (NEMBA) None unpubl. data), and stricter translocation policies should be CITES listing None established to prevent the mixing of subspecies. Overall, this subspecies could become a keystone in the Endemic Yes sustainable wildlife economy. The common name, Blesbok, originates from ‘Bles’, the Afrikaans word for a ‘blaze’, which Distribution symbolises the white facial marking running down Historically, the Blesbok ranged across the Highveld from the animal’s horns to its nose, broken only grasslands of the Free State and Gauteng provinces, by the brown band above the eyes (Skinner & extending into northwestern KwaZulu-Natal, and through Chimimba 2005). -
Muskox Management Report Alaska Dept of Fish and Game Wildlife
Muskox Management Report of survey-inventory activities 1 July 1998–30 June 2000 Mary V. Hicks, Editor Alaska Department of Fish and Game Division of Wildlife Conservation December 2001 ADF&G Please note that population and harvest data in this report are estimates and may be refined at a later date. If this report is used in its entirety, please reference as: Alaska Department of Fish and Game. 2001. Muskox management report of survey-inventory activities 1 July 1998–30 June 2000. M.V. Hicks, editor. Juneau, Alaska. If used in part, the reference would include the author’s name, unit number, and page numbers. Authors’ names can be found at the end of each unit section. Funded in part through Federal Aid in Wildlife Restoration, Proj. 16, Grants W-27-2 and W-27-3. LOCATION 2 GAME MANAGEMENT UNIT: 18 (41,159 mi ) GEOGRAPHIC DESCRIPTION: Yukon–Kuskokwim Delta BACKGROUND NUNIVAK ISLAND Muskoxen were once widely distributed in northern and western Alaska but were extirpated by the middle or late 1800s. In 1929, with the support of the Alaska Territorial Legislature, the US Congress initiated a program to reintroduce muskoxen in Alaska. Thirty-one muskoxen were introduced from Greenland to Nunivak Island in Unit 18 during 1935–1936, as a first step toward reintroducing this species to Alaska. The Nunivak Island population grew slowly until approximately 1958 and then began a period of rapid growth. The first hunting season was opened in 1975, and the population has since fluctuated between 400 and 750 animals, exhibiting considerable reproductive potential, even under heavy harvest regimes. -
Fitzhenry Yields 2016.Pdf
Stellenbosch University https://scholar.sun.ac.za ii DECLARATION By submitting this dissertation electronically, I declare that the entirety of the work contained therein is my own, original work, that I am the sole author thereof (save to the extent explicitly otherwise stated), that reproduction and publication thereof by Stellenbosch University will not infringe any third party rights and that I have not previously in its entirety or in part submitted it for obtaining any qualification. Date: March 2016 Copyright © 2016 Stellenbosch University All rights reserved Stellenbosch University https://scholar.sun.ac.za iii GENERAL ABSTRACT Fallow deer (Dama dama), although not native to South Africa, are abundant in the country and could contribute to domestic food security and economic stability. Nonetheless, this wild ungulate remains overlooked as a protein source and no information exists on their production potential and meat quality in South Africa. The aim of this study was thus to determine the carcass characteristics, meat- and offal-yields, and the physical- and chemical-meat quality attributes of wild fallow deer harvested in South Africa. Gender was considered as a main effect when determining carcass characteristics and yields, while both gender and muscle were considered as main effects in the determination of physical and chemical meat quality attributes. Live weights, warm carcass weights and cold carcass weights were higher (p < 0.05) in male fallow deer (47.4 kg, 29.6 kg, 29.2 kg, respectively) compared with females (41.9 kg, 25.2 kg, 24.7 kg, respectively), as well as in pregnant females (47.5 kg, 28.7 kg, 28.2 kg, respectively) compared with non- pregnant females (32.5 kg, 19.7 kg, 19.3 kg, respectively). -
Beatragus Hunteri) in Arawale National Reserve, Northeastern, Kenya
The population size, abundance and distribution of the Critically Endangered Hirola Antelope (Beatragus hunteri) in Arawale National Reserve, Northeastern, Kenya. Francis Kamau Muthoni Terra Nuova, Transboundary Environmental Project, P.O. Box 74916, Nairobi, Kenya Email: [email protected] 1.0. Abstract. This paper outlines the spatial distribution, population size, habitat preferences and factors causing the decline of Hirola antelope in Arawale National Reserve (ANR) in Garissa and Ijara districts, north eastern Kenya. The reserve covers an area of 540Km2. The objectives of the study were to gather baseline information on hirola distribution, population size habitat preferences and human activities impacting on its existence. A sampling method using line transect count was used to collect data used to estimate the distribution of biological populations (Norton-Griffiths, 1978). Community scouts collected data using Global Positioning Systems (GPS) and recorded on standard datasheets for 12 months. Transect walks were done from 6.00Am to 10.00Am every 5th day of the month. The data was entered into a geo-database and analysed using Arcmap, Ms Excel and Access. The results indicate that the population of hirola in Arawale National Reserve were 69 individuals comprising only 6% of the total population in the natural geographic range of hirola estimated to be 1,167 individuals. It also revealed that hirola prefer open bushes and grasslands. The decline of the Hirola on its natural range is due to a combination of factors, including, habitat loss and degradation, competition with livestock, poaching and drought. Key words: Hirola Antelope Beatragus hunteri, GIS, Endangered Species 2.0. Introduction. The Hirola antelope (Beatragus hunteri) is a “Critically Endangered” species endemic to a small area in Southeast Kenya and Southwest Somalia. -
African Mammals (Tracks)
L Gi'. M MM S C A POCKET NATURALISru GUIDE HOOFED MAMMALS Dik-Dik Madoqua spp . To 17 in. (43 cm) H Small antelope has a long, flexible snout. .,9-10 in. Common Hippopotamus Klipspringer Hippopotamus amphibius Oreatragus oreotroqus To 5 ft. (1.5 m) H To 2 ft. (60 cm) H Has dark 'tear stains' at the corner of the eyes. Downward-pointing hooves give the impression it walks on 'tiptoe'. Found in rocky habitats. White Black Steenbok Raphieerus eampestris 1 in. White Rhinoceros To 2 ft. (60 cm) H Large ears are striped inside. Ceratotherium simum Muzzle has a dark stripe. To 6 ft. (l.B m) H 9-10 in. t Has a square upper lip. The similar black rhinoceros has a , pointed, prehensile upper lip. t . eWhite '~'rBlack . Common Duiker " . Sylvieapra grimmia ". To 28 in. (70 cm) t1 t Has a prominent black 1 in. 'a: stripe on its snout. Inhabits woodlands Hyena lion and shrubby areas. t Forefoot , e+ Oribi 24-28 in. Ourebia ourebi African Elephant To 2 ft. (60 em) H Note short tail and black 1.5 in. Loxodonto africana Hind foot spot below ears. Inhabits To 14 ft. (4.2 m) H grassland savannas. Hind print is oval-shaped. t This guide provides simplified field reference to familiar animal tracks. It is important to note that tracks change depending on their age, the surface Hippopotamus they are made on, and the animal's gait (e.g., toes are often splayed when Springbok running). Track illustrations are ordered by size in each section and are not Antidorcas marsupialis To 30 in. -
What Do Caribou and Wood Bison Have in Common? by Nate Olson
Refuge Notebook • Vol. 17, No. 4 • January 23, 2015 What do caribou and wood bison have in common? by Nate Olson Residents from the village of Shageluk on the Innoko River assist ADFG and USFWS staff in constructing a holding pen as part of the wood bison reintroduction effort. 100 wood bison are scheduled to be released on the lower Innoko River in March 2015. Photo credit: Tom Seaton, ADFG. Last weekend on a drive back from Anchorage, Wood bison are native to Alaska and were plentiful whizzing by the Alaska Wildlife Conservation Center over a large portion of the state until their extirpation at 57 miles per hour my daughter pointed out a strange in the early 1900s. The reasons for their disappearance looking moose on the side of the Seward Highway. are not clearly known but probably related to overhar- “That isn’t a moose,” I explained, “that is a woodbi- vest and habitat loss. son.” Wildlife transplants are nothing new in Alaska. And why are wood bison living in pens next to The reasons for transplanting animals generally fall the Seward Highway? They are part of a reintroduc- into two categories. The first is to provide human re- tion effort by the Alaska Department of Fish and Game lated benefits such as recreational hunting, economic (ADFG) and the Alaska Wildlife Conservation Center. gain, or an additional food supply. The second is re- The fate of these wood bison has been in the news lated to species recovery in their historic range. lately as the U.S. Fish and Wildlife Service (USFWS) Kodiak Island is a dramatic example of the first published a final rule in May 2014 that gives the green category. -
Ecology of Red Deer a Research Review Relevant to Their Management in Scotland
Ecologyof RedDeer A researchreview relevant to theirmanagement in Scotland Instituteof TerrestrialEcology Natural EnvironmentResearch Council á á á á á Natural Environment Research Council Institute of Terrestrial Ecology Ecology of Red Deer A research review relevant to their management in Scotland Brian Mitchell, Brian W. Staines and David Welch Institute of Terrestrial Ecology Banchory iv Printed in England by Graphic Art (Cambridge) Ltd. ©Copyright 1977 Published in 1977 by Institute of Terrestrial Ecology 68 Hills Road Cambridge CB2 11LA ISBN 0 904282 090 Authors' address: Institute of Terrestrial Ecology Hill of Brathens Glassel, Banchory Kincardineshire AB3 4BY Telephone 033 02 3434. The Institute of Terrestrial Ecology (ITE) was established in 1973, from the former Nature Conservancy's research stations and staff, joined later by the Institute of Tree Biology and the Culture Centre of Algae and Protozoa. ITE contributes to and draws upon the collective knowledge of the fourteen sister institutes which make up the Natural Environment Research Council, spanning all the environmental sciences. The Institute studies the factors determining the structure, composition and processes of land and freshwater systems, and of individual plant and animal species. It is developing a Sounder scientific basis for predicting and modelling environmental trends arising from natural or man-made change. The results of this research are available to those responsible for the protection, management and wise use of our natural resources. Nearly half of ITE'Swork is research commissioned by customers, such as the Nature Conservancy Council who require information for wildlife conservation, the Forestry Commission and the Department of the Environment. The remainder is fundamental research supported by NERC. -
The Preparation and Primary Structure of S-Peptides from Different Pancreatic Ribonucleases
CORE Metadata, citation and similar papers at core.ac.uk Provided by Elsevier - Publisher Connector Volume 40, number 1 FEBS LETTERS March 1974 THE PREPARATION AND PRIMARY STRUCTURE OF S-PEPTIDES FROM DIFFERENT PANCREATIC RIBONUCLEASES G.W. WELLING, G. GROEN, D. GABEL+, W. GAASTRA, J.J. BEINTEMA Biochemisch Laboratorium, Rijksuniversiteit, Zernikelaan, Groningen, The Netherlands Received 14 December 1973 1. Introduction Miles-Seravac Ltd. (Maidenhead). All other ribonu- cleases used in this study (goat, giraffe, gnu, reindeer, In 1955, Richards [l] described the isolation of dromedary, kangaroo, lesser rorqual, pig, and horse) ‘an active intermediate produced during the digestion were isolated according to Wierenga et al. [7] and rat of ribonuclease by subtilisin’. The characterisation RNase, according to Beintema et al. [8]. Subtilopep- and separation of the non-covalently linked compo- tidase A (Subtilisin Carlsberg) was a gift from Novo nents was described 4 years later [2] . Ribonuclease Industri (Copenhagen). Sephadex G-50 (fine) was S* possesses full enzymatic activity and the same purchased from Pharmacia (Uppsala). All other rea- holds for the enzyme reconstituted from S-peptide gents were analytical grade products from Merck AG and S-protein. The involvement of S-peptide residues (Darmstadt). in the binding of S-peptide to S-protein and in the Amino acid analysis, high-voltage paper electro- enzymatic activity of the reconstituted RNase S’ has phoresis, dansylation, and dansyl-Edman degrada- been studied by using synthetic S-peptide analogs [3,4] tion were performed as described earlier [7, 93. the cleavage by subtilisin takes place in an external loop. Klee [5] and Gold [6] did not succeed in 2.1.