DDX20 Antibody Cat. No.: 19-877
DDX20 Antibody
Immunohistochemistry of paraffin-embedded rat kidney Immunohistochemistry of paraffin-embedded human using DDX20 Antibody (19-877) at dilution of 1:100 (40x amygdalitis using DDX20 Antibody (19-877) at dilution of lens). 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human Immunofluorescence analysis of HeLa cells using DDX20 colon using DDX20 Antibody (19-877) at dilution of 1:100 antibody (19-877). Blue: DAPI for nuclear staining. (40x lens).
Specifications
HOST SPECIES: Rabbit
October 1, 2021 1 https://www.prosci-inc.com/ddx20-antibody-19-877.html SPECIES REACTIVITY: Human, Mouse, Rat
Recombinant fusion protein containing a sequence corresponding to amino acids 25-280 IMMUNOGEN: of human DDX20 (NP_009135.4).
TESTED APPLICATIONS: IF, IHC, WB
WB: ,1:500 - 1:2000
APPLICATIONS: IHC: ,1:50 - 1:200
IF: ,1:10 - 1:100
POSITIVE CONTROL: 1) MCF7
2) ES-2
3) 293T
4) HepG2
5) HeLa
6) Mouse spleen
PREDICTED MOLECULAR Observed: 110kDa WEIGHT:
Properties
PURIFICATION: Affinity purification
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.
Additional Info
OFFICIAL SYMBOL: DDX20
Probable ATP-dependent RNA helicase DDX20, Component of gems 3, DEAD box protein ALTERNATE NAMES: 20, DEAD box protein DP 103, Gemin-3, DDX20, DP103, GEMIN3
GENE ID: 11218
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
October 1, 2021 2 https://www.prosci-inc.com/ddx20-antibody-19-877.html Background and References
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in BACKGROUND: embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.
ANTIBODIES FOR RESEARCH USE ONLY.
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October 1, 2021 3 https://www.prosci-inc.com/ddx20-antibody-19-877.html