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Tracheal Wash Vs BAL 2 TRACHEAL WASHES IN DOGS AND CATS: WHY, WHAT, WHEN, AND HOW Eleanor C. Hawkins, DVM, Dipl ACVIM (SAIM) Professor, Small Animal Internal Medicine North Carolina State University Raleigh, North Carolina, USA 1. Introduction: Tracheal Wash vs BAL 2. Focus on Tracheal Wash TRACHEAL WASH vs BAL 1 TRACHEAL WASH vs BAL • Exudate from airways and alveoli • Samples all alveoli dependent on to the trachea via mucociliary the bronchus where scope or clearance +/‐ cough. catheter is lodged. • Good representation for most • Primarily a deep lung sample: diffuse bronchial disease and small airways, alveoli, and aspiration or bronchopneumonia. sometimes the interstitium. TRACHEAL WASH vs BAL TRACHEAL WASH vs BAL 2 DEFINITIONS – Slippery slope • TW becomes BAL • Catheter into small airways • Relatively large volumes of fluid used • BAL becomes TW • Single bolus • Relatively small volume Regardless of method • Tracheal wash (TW) and bronchoalveolar lavage (BAL) result in sufficient material for: Cytology Cultures PCR Flow cytometry Special stains / markers Cell function testing • BAL: greater volume, more cells than TW TW and BAL Cytology • Similar benefits › Less invasive than getting tissue 3 TW and BAL Cytology • Similar limitations › No architecture › Cells must exfoliate • E.g. not pulmonary fibrosis • E.g. not sarcoma › Organisms must be present in large numbers › Secondary processes must not “hide” primary • Infection vs non‐infectious disease • Inflammation vs neoplasia TW and BAL • MOST USEFUL FOR • Ruling IN infectious disease • Ruling IN neoplasia • CAN HELP PRIORITIZE DIFFERENTIAL DIAGNOSES Tracheal Wash • Indications • Bronchial and alveolar disease • Because of safety, may consider for any lung disease • Less likely to be representative of interstitial or local processes 4 Tracheal Wash • Indications • Bronchial and alveolar disease • CHRONIC COUGH (BRONCHITIS) • BRONCHOPNEUMONIA / ASPIRATION PNEUMONIA • Majority of dogs and cats requiring airway sampling!! Bronchoalveolar Lavage • Indications • Primarily: Interstitial / deep lung disease • Neoplasia • Systemic fungal infection • Eosinophilic lung disease • Bronchoscopic collection • Directed sampling TRACHEAL WASH vs BAL – WHICH IS BETTER? 1. Is bronchoscopy indicated? 2. Where is the disease within the lung? 3. What are the top differential diagnoses? 4. How stable is the patient? 5. How risk‐averse are the clients? 6. What are the financial constraints? 5 TRACHEAL WASH vs BAL – WHICH IS BETTER? 3. What are the top differential diagnoses • If neoplasia or systemic fungal disease, BAL more likely to provide diagnostic specimen • Unsure for other infectious agents such as parasites • Aelurostrongylus best found on fecal Baermann exam! TRACHEAL WASH vs BAL –WHICH IS BETTER? 1. Is bronchoscopy indicated 2. Where is the disease within the lung? 3. What are the top differential diagnoses 4. How stable is the patient 5. How risk‐averse are the clients 6. What are the financial constraints Tracheal Wash: Bronchial and/or alveolar disease • Signs of bronchitis – usually cough, consistent radiographs (bronchial pattern or unremarkable) • Confirm inflammation • Characterize inflammation • Identify organisms (cytology, bacterial culture, +/‐ mycoplasma culture and/or PCR) • Culture and antibiotic sensitivity information 6 Tracheal Wash: Bronchial and/or alveolar disease • Signs of bacterial bronchopneumonia • Confirm septic inflammation • Culture and antibiotic sensitivity information TRANS‐TRACHEAL WASH (TTW) vs ENDOTRACHEAL (ETW) By‐passing the oral cavity TRANSTRACHEAL WASH ENDOTRACHEAL WASH • Moderately difficult to perform • Quite challenging in small dogs and cats due to size of target • Only requires sedation and local anesthesia • Coughing may improve yield 7 TRANSTRACHEAL WASH ENDOTRACHEAL WASH • Moderately difficult to • Technically easy perform • Safer for small dogs and • Quite challenging in small cats dogs and cats due to size • Requires general of target anesthesia • Only requires sedation • Greater ability to adjust and local anesthesia positioning of catheter • Coughing may improve yield Endotracheal wash: Great if needs anesthesia for other reason! Getting the best TW results 1. DO THEM! 2. Select appropriate patient at the appropriate time 3. Practice tips for maximizing results 8 Getting the best TW results: Review steps if it has been awhile 3rd edition 6th edition due out available Sept 2020 now Be prepared in advance Considerations: ET then TTW for each • Patient Prep • Catheter diameter • Catheter length • Avoiding contamination • Volume of saline / number of boluses • Handling of fluid 9 Endo‐tracheal wash – Eezy Peezy • Patient under light anesthesia • e.g. butorphanol / propofol in small dogs • e.g. ketamine / midazolam in cats • See anesthesiology • Generally cats and small dogs • Can collect from patient already under anesthesia Bronchodilators: PRE‐MED CATS! • Usual dose of theophylline • Oral aminophylline 30‐60 min prior to procedure ‐OR ‐ • SQ terbutaline 10 min prior to procedure ‐AND/OR ‐ 10 TTW: Prevent Movement Without Suppressing Cough (if possible) • Need to avoid movement • Tracheal laceration • “Walk off” catheter • Avoid narcotics if possible (cough suppressant) • Acepromazine • 0.05 – 0.1 mg/kg IV or SQ • Wait 10‐15 min (OR ‐ low dose of butorphanol; dexmedetomidine) • Lidocaine locally TTW: Prevent Movement Without Suppressing Cough (if possible) • Need to avoid movement • Ideally 5 people! • Tracheal laceration • 1 to hold head • “Walk off” catheter • 1 to hold body • Avoid narcotics if possible (cough suppressant) • 1 to pass cannula and • Acepromazine hold in position • 0.05 – 0.1 mg/kg IV or SQ • 1 to pass flushing • Wait 10‐15 min catheter and perform (OR ‐ low dose of butorphanol; lavage dexmedetomidine) • 1 to pass and cap • Lidocaine locally syringes Considerations • Patient Prep • Catheter diameter • Catheter length • Avoiding contamination • Volume of saline / number of boluses • Handling of fluid 11 Size of catheter • SMALLER is generally BETTER! • Start with 3.5 – 5 Fr Tracheal wash: Size of catheter • SMALLER is generally BETTER! • Want fluid and mucus, not air • Start with 3.5 – 5 Fr Transtracheal wash catheters: thru the needle 8 or 12” 12 MILA® Canula with flush catheter TTW with ability to control insertion length MILA® Canula with flush catheter Considerations • Patient Prep • Catheter diameter • Catheter length • Avoiding contamination • Volume of saline / number of boluses • Handling of fluid 13 Tracheal wash: Length is important! Size tube: 4.0 I.D. Size tube: 10.0 I.D. Size catheter: 5 Fr red rubber Size catheter: 5 Fr red rubber Needs to extend several cm beyond end of ET tube 14 Longer catheters • Small diameter nasogastric tubes • Polypropylene male dog urinary catheters VISUALIZE ANATOMY FULLY X 1. Where is the carina? – Aiming for just proximal 2. How far should I pass the ET tube? ‐ Just past the larynx 3. How far should I pass the wash catheter? – Beyond the ET tube but before the carina Length of catheter • Relative to length of ET tube • ALSO relative to depth into the lung • Aim for just in front of the carina • Carina is at about the 4th intercostal space 15 Visualize Anatomy Fully for TTW, too! • Do I have the right length catheter? • With Mila‐type system, can adjust the length during the procedure More versatility Requires similar anatomical considerations as for ETW Carina ≈ 4th ICS 16 17 Considerations • Patient Prep • Catheter diameter • Catheter length • Avoiding contamination • Volume of saline / number of boluses • Handling of fluid Intubation – Minimize Contamination • Use sterile endotracheal tube • Do not touch either end • Put lidocaine on larynx • Use a laryngoscope 18 X GLOVES 3‐WAY STOPCOCK 19 Minimize Contamination • Glycopyrrolate or Atropine? • Surgical prep • Care with hands • Gloves don’t stay sterile • Touch neck • Touch syringes Non‐Dominant Hand: Hold larynx well • Size of airway • Prevent rolling of trachea or slipping off side • Once catheter in place, maintain contact between catheter and neck 20 BEVEL OF NEEDLE “DOWN” Considerations • Patient Prep • Catheter diameter • Catheter length • Avoiding contamination • Volume of saline / number of boluses • Handling of fluid Number of Instillation Boluses? Volume? 21 Instillation Volume and bolus numbersVolume? • Generally start with 3‐5 ml boluses to find “sweet spot” • Adapt if needed to improve return • BASE ON RESULTS • Visibly turbid fluid • Sufficient volume Endo‐tracheal wash – VIDEO NOTE: Suction or drain excess fluid from endotracheal tube when finished so that fluid does not obstruct ventilation through the endotracheal tube! Considerations • Patient Prep • Catheter diameter • Catheter length • Avoiding contamination • Volume of saline / number of boluses • Handling of fluid 22 Now What? •Cytologic Examination •Aerobic culture •Mycoplasma? • PCR • Culture •Anaerobes? •Actinomycetes? •Nocardia? CYTOLOGIC EXAMINATION • CLASSIFICATION OF CELL POPULATION • QUALITATIVE EVALUATION OF CELLS • MACROPHAGE ACTIVATION • NEUTROPHIL DEGENERATION • CRITERIA OF MALIGNANCY • ETIOLOGIC AGENTS • BACTERIA • PROTOZOA • FUNGI • PARASITES Cytospin or Sediment Prep 23 PRACTICE TIP: MAKE SLIDES OF MUCUS •May find trapped organisms! PRACTICE TIP: MAKE SLIDES OF MUCUS •May concentrate organisms •Saline likely to disperse over time •Make the slides yourself •Poor for cellular characteristics Need BOTH cytospin AND mucus squash 24 Not happening for you? Picture the Anatomy and Change Something! •Position of catheter •Position of patient •Volume of saline •Size of catheter THE END 25.
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