Supplementary Appendix: a High Level of Inter-Genera Gene
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Identification of Early Salinity Stress-Responsive Proteins In
International Journal of Molecular Sciences Article Identification of Early Salinity Stress-Responsive Proteins in Dunaliella salina by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis Yuan Wang 1,2,†, Yuting Cong 2,†, Yonghua Wang 3, Zihu Guo 4, Jinrong Yue 2, Zhenyu Xing 2, Xiangnan Gao 2 and Xiaojie Chai 2,* 1 Key Laboratory of Hydrobiology in Liaoning Province’s Universities, Dalian Ocean University, Dalian 116021, China; [email protected] 2 College of fisheries and life science, Dalian Ocean University, Dalian 116021, China; [email protected] (Y.C.); [email protected] (J.Y.); [email protected] (Z.X.); [email protected] (X.G.) 3 Bioinformatics Center, College of Life Sciences, Northwest A&F University, Yangling 712100, China; [email protected] 4 College of Life Sciences, Northwest University, Xi’an, Shaanxi 710069, China; [email protected] * Correspondence: [email protected] † These authors contribute equally to the work. Received: 22 November 2018; Accepted: 16 January 2019; Published: 30 January 2019 Abstract: Salt stress is one of the most serious abiotic factors that inhibit plant growth. Dunaliella salina has been recognized as a model organism for stress response research due to its high capacity to tolerate extreme salt stress. A proteomic approach based on isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the proteome of D. salina during early response to salt stress and identify the differentially abundant proteins (DAPs). A total of 141 DAPs were identified in salt-treated samples, including 75 upregulated and 66 downregulated DAPs after 3 and 24 h of salt stress. -
Phylogenetic Study of Some Strains of Dunaliella
American Journal of Environmental Science 9 (4): 317-321, 2013 ISSN: 1553-345X ©2013 Science Publication doi:10.3844/ajessp.2013.317.321 Published Online 9 (4) 2013 (http://www.thescipub.com/ajes.toc) Phylogenetic Study of Some Strains of Dunaliella 1Duc Tran, 1Trung Vo, 3Sixto Portilla, 2Clifford Louime, 1Nguyen Doan, 1Truc Mai, 1Dat Tran and 1Trang Ho 1School of Biotechnology, International University, Thu Duc Dist., VNU, Vietnam 2Florida A and M University, College of Engineering Sciences, Technology and Agriculture, FAMU Bio Energy Group, Tallahassee, FL 32307, USA 3Center for Estuarine, Environmental and Coastal Oceans Monitoring, Dowling College, 150 Idle Hour Blvd, Oakdale, New York 11769, USA Received 2013-07-13, Revised 2013-08-02; Accepted 2013-08-03 ABSTRACT Dunaliella strains were isolated from a key site for salt production in Vietnam (Vinh Hao, Binh Thuan province). The strains were identified based on Internal Transcribed Spacer (ITS) markers. The phylogenetic tree revealed these strains belong to the clades of Dunaliella salina and Dunaliella viridis . Results of this study confirm the ubiquitous nature of Dunaliella and suggest that strains of Dunaliella salina might be acquired locally worldwide for the production of beta-carotene. The identification of these species infers the presence of other Dunaliella species ( Dunaliella tertiolecta , Dunaliella primolecta , Dunaliella parva ), but further investigation would be required to confirm their presence in Vietnam. We anticipate the physiological and biochemical characteristics of these local species will be compared with imported strains in a future effort. This will facilitate selection of strains with the best potential for exploitation in the food, aquaculture and biofuel industries. -
The Ecology of Dunaliella in High-Salt Environments Aharon Oren
Oren Journal of Biological Research-Thessaloniki (2014) 21:23 DOI 10.1186/s40709-014-0023-y REVIEW Open Access The ecology of Dunaliella in high-salt environments Aharon Oren Abstract Halophilic representatives of the genus Dunaliella, notably D. salina and D. viridis, are found worldwide in salt lakes and saltern evaporation and crystallizer ponds at salt concentrations up to NaCl saturation. Thanks to the biotechnological exploitation of D. salina for β-carotene production we have a profound knowledge of the physiology and biochemistry of the alga. However, relatively little is known about the ecology of the members of the genus Dunaliella in hypersaline environments, in spite of the fact that Dunaliella is often the main or even the sole primary producer present, so that the entire ecosystem depends on carbon fixed by this alga. This review paper summarizes our knowledge about the occurrence and the activities of different Dunaliella species in natural salt lakes (Great Salt Lake, the Dead Sea and others), in saltern ponds and in other salty habitats where members of the genus have been found. Keywords: Dunaliella, Hypersaline, Halophilic, Great Salt Lake, Dead Sea, Salterns Introduction salt adaptation. A number of books and review papers When the Romanian botanist Emanoil C. Teodoresco have therefore been devoted to the genus [5-7]. How- (Teodorescu) (1866–1949) described the habitat of the ever, the ecological aspects of the biology of Dunaliella new genus of halophilic unicellular algae Dunaliella,it are generally neglected. A recent monograph did not was known from salterns and salt lakes around the devote a single chapter to ecological aspects, and con- Mediterranean and the Black Sea [1-3]. -
Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341 -
Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species from an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme
ORIGINAL RESEARCH published: 20 August 2020 doi: 10.3389/fpls.2020.01259 Glycerol Is an Osmoprotectant in Two Antarctic Chlamydomonas Species From an Ice-Covered Saline Lake and Is Synthesized by an Unusual Bidomain Enzyme † James A. Raymond 1*, Rachael Morgan-Kiss 2 and Sarah Stahl-Rommel 2 1 School of Life Sciences, University of Nevada Las Vegas, Las Vegas, NV, United States, 2 Department of Microbiology, Miami University, Oxford, OH, United States Glycerol, a compatible solute, has previously been found to act as an osmoprotectant in Edited by: some marine Chlamydomonas species and several species of Dunaliella from hypersaline Linda Nedbalova´ , Charles University, Czechia ponds. Recently, Chlamydomonas reinhardtii and Dunaliella salina were shown to make Reviewed by: glycerol with an unusual bidomain enzyme, which appears to be unique to algae, that Aharon Oren, contains a phosphoserine phosphatase and glycerol-3-phosphate dehydrogenase. Here Hebrew University of Jerusalem, Israel David Dewez, we report that two psychrophilic species of Chlamydomonas (C. spp. UWO241 and ICE- Universite´ du Que´ bec à Montre´ al, MDV) from Lake Bonney, Antarctica also produce high levels of glycerol to survive in the Canada lake’s saline waters. Glycerol concentration increased linearly with salinity and at 1.3 M *Correspondence: NaCl, exceeded 400 mM in C. sp. UWO241, the more salt-tolerant strain. We also show James A. Raymond [email protected] that both species expressed several isoforms of the bidomain enzyme. An analysis of one †Present address: of the isoforms of C. sp. UWO241 showed that it was strongly upregulated by NaCl and is Sarah Stahl-Rommel thus the likely source of glycerol. -
The Symbiotic Green Algae, Oophila (Chlamydomonadales
University of Connecticut OpenCommons@UConn Master's Theses University of Connecticut Graduate School 12-16-2016 The yS mbiotic Green Algae, Oophila (Chlamydomonadales, Chlorophyceae): A Heterotrophic Growth Study and Taxonomic History Nikolaus Schultz University of Connecticut - Storrs, [email protected] Recommended Citation Schultz, Nikolaus, "The yS mbiotic Green Algae, Oophila (Chlamydomonadales, Chlorophyceae): A Heterotrophic Growth Study and Taxonomic History" (2016). Master's Theses. 1035. https://opencommons.uconn.edu/gs_theses/1035 This work is brought to you for free and open access by the University of Connecticut Graduate School at OpenCommons@UConn. It has been accepted for inclusion in Master's Theses by an authorized administrator of OpenCommons@UConn. For more information, please contact [email protected]. The Symbiotic Green Algae, Oophila (Chlamydomonadales, Chlorophyceae): A Heterotrophic Growth Study and Taxonomic History Nikolaus Eduard Schultz B.A., Trinity College, 2014 A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of Master of Science at the University of Connecticut 2016 Copyright by Nikolaus Eduard Schultz 2016 ii ACKNOWLEDGEMENTS This thesis was made possible through the guidance, teachings and support of numerous individuals in my life. First and foremost, Louise Lewis deserves recognition for her tremendous efforts in making this work possible. She has performed pioneering work on this algal system and is one of the preeminent phycologists of our time. She has spent hundreds of hours of her time mentoring and teaching me invaluable skills. For this and so much more, I am very appreciative and humbled to have worked with her. Thank you Louise! To my committee members, Kurt Schwenk and David Wagner, thank you for your mentorship and guidance. -
MICROBIOLOGICAL and ECOPHYSIOLOGICAL CHARACTERIZATION of GREEN ALGAE Dunaliella Sp
MICROBIOLOGICAL AND ECOPHYSIOLOGICAL CHARACTERIZATION OF GREEN ALGAE Dunaliella sp. FOR IMPROVEMENT OF CAROTENOID PRODUCTION Muhammad Zainuri 1) Hermin Pancasakti Kusumaningrum*2 ), and Endang Kusdiyantini 2), 1) Laboratory of Biological Oceanography, Department of marine Sciences, Faculty of Fisheries and Marine Sciences, Diponegoro University 2) Microbiogenetics Laboratory, Faculty of Mathematics and Natural Sciences, Diponegoro University, Jl. Prof. Soedarto, UNDIP, Tembalang, Semarang. 50275. e-mail : [email protected] Abstract An isolate of green algae Dunaliella sp. from BBAP Jepara is usually used as a source for carotenoid supplement for marine animal cultivation in the local area. In order to improve carotenoid production especially detection of biosynthetic pathway from the organisms investigated in this study, the main purpose of this study is characterizing Dunaliella sp. based on it’s microbiological and ecophysiological characters. The research was done by characterize the growth, the cell and colonies microbiologically, total pigment production, and also characterize all of the ecophysiological factors affecting the algal growth and survival. The results of this research showed that Dunaliella sp. posseses typical characteristic of green eucaryote alga, in their growth and ecological condition. The extreme characters which was toleration ability to high salinity environment of was used to conclude Dunaliella sp. as Dunaliella salina. Key words : algae, Dunaliella sp. , microbiological, ecophysiological, characterization Introduction serve as precursors of many hormones Green algae are simple (Vershinin, 1999 in Lee and Schmidt- photosynthetic eukaryotes which are Dannert, 2002). Carotenoids are used responsible for up to 50% of the planet's commercially as food colorants, animal atmospheric carbon fixation. The recent feed supplements and, more recently, as discoveries of health related beneficial nutraceuticals for cosmetic and properties attributed to algal carotenoids pharmaceutical purposes. -
"Phycology". In: Encyclopedia of Life Science
Phycology Introductory article Ralph A Lewin, University of California, La Jolla, California, USA Article Contents Michael A Borowitzka, Murdoch University, Perth, Australia . General Features . Uses The study of algae is generally called ‘phycology’, from the Greek word phykos meaning . Noxious Algae ‘seaweed’. Just what algae are is difficult to define, because they belong to many different . Classification and unrelated classes including both prokaryotic and eukaryotic representatives. Broadly . Evolution speaking, the algae comprise all, mainly aquatic, plants that can use light energy to fix carbon from atmospheric CO2 and evolve oxygen, but which are not specialized land doi: 10.1038/npg.els.0004234 plants like mosses, ferns, coniferous trees and flowering plants. This is a negative definition, but it serves its purpose. General Features Algae range in size from microscopic unicells less than 1 mm several species are also of economic importance. Some in diameter to kelps as long as 60 m. They can be found in kinds are consumed as food by humans. These include almost all aqueous or moist habitats; in marine and fresh- the red alga Porphyra (also known as nori or laver), an water environments they are the main photosynthetic or- important ingredient of Japanese foods such as sushi. ganisms. They are also common in soils, salt lakes and hot Other algae commonly eaten in the Orient are the brown springs, and some can grow in snow and on rocks and the algae Laminaria and Undaria and the green algae Caulerpa bark of trees. Most algae normally require light, but some and Monostroma. The new science of molecular biology species can also grow in the dark if a suitable organic carbon has depended largely on the use of algal polysaccharides, source is available for nutrition. -
Competitive Exclusion of Cyanobacterial Species in the Great Salt Lake
Brigham Young University BYU ScholarsArchive Theses and Dissertations 2008-07-10 Competitive Exclusion of Cyanobacterial Species in the Great Salt Lake Hillary Christine Roney Brigham Young University - Provo Follow this and additional works at: https://scholarsarchive.byu.edu/etd Part of the Animal Sciences Commons BYU ScholarsArchive Citation Roney, Hillary Christine, "Competitive Exclusion of Cyanobacterial Species in the Great Salt Lake" (2008). Theses and Dissertations. 1826. https://scholarsarchive.byu.edu/etd/1826 This Thesis is brought to you for free and open access by BYU ScholarsArchive. It has been accepted for inclusion in Theses and Dissertations by an authorized administrator of BYU ScholarsArchive. For more information, please contact [email protected], [email protected]. Competitive Exclusion of Cyanobacterial Species in the Great Salt Lake by Hillary C. Roney A thesis submitted to the faculty of Brigham Young University in partial fulfillment of the requirements for the degree of Master of Science Department of Plant and Wildlife Sciences Brigham Young University August 2008 Copyright © 2008 Hillary C. Roney All Rights Reserved BRIGHAM YOUNG UNIVERSITY GRADUATE COMMITTEE APPROVAL of a thesis submitted by Hillary C. Roney This thesis has been read by each member of the following graduate committee and by majority vote has been found to be satisfactory. Date Gary M. Booth, Chair Date Donald P. Breakwell Date John Gardner Date Bruce Schaalje BRIGHAM YOUNG UNIVERSITY As chair of the candidate‟s graduate committee, I have read the thesis of Hillary C. Roney in its final form and have found that (1) its format, citations, and bibliographical style are consistent and acceptable and fulfill university and department style requirements; (2) its illustrative materials including figures, tables, and charts are in place; and (3) the final manuscript is satisfactory to the graduate committee and is ready for submission to the university library. -
Halomonas Maura Sp. Nov., a Novel Moderately Halophilic, Exopolysaccharide-Producing Bacterium
International Journal of Systematic and Evolutionary Microbiology (2001), 51, 1625–1632 Printed in Great Britain Halomonas maura sp. nov., a novel moderately halophilic, exopolysaccharide-producing bacterium Microbial Samir Bouchotroch, Emilia Quesada, Ana del Moral, Inmaculada Llamas Exopolysaccharides Research Group, Department of and Victoria Be! jar Microbiology, Faculty of Pharmacy, Campus Universitario de Cartuja, Author for correspondence: Emilia Quesada. Tel: j34 958 243871. Fax: j34 958 246235. University of Granada, e-mail: equesada!platon.ugr.es 18071 Granada, Spain Four moderately halophilic, exopolysaccharide-producing bacterial strains isolated from soil samples collected from a saltern at Asilah (Morocco) are reported. These four strains were initially considered to belong to the genus Halomonas. Their DNA GMC contents varied between 622 and 641 mol%. DNA–DNA hybridization revealed a considerable degree of DNA–DNA similarity amongst all four strains (755–808%). Nevertheless, similarity with the reference strains of phylogenetically close relatives was lower than 40%. 16S rRNA gene sequences were compared with those of other species of Halomonas and other Gram-negative bacteria and they were sufficiently distinct phylogenetically from other recognized Halomonas species to warrant their designation as a novel species. The name Halomonas maura sp. nov. is therefore proposed, with strain S-31T (l CECT 5298T l DSM 13445T) as the type strain. The fatty acid composition of strain S-31T revealed the presence of 18:1ω7c,16:1ω7c/2-OH i15:0 and 16:0 as the major components. Growth rate analysis showed that strain S-31T had specific cationic requirements for NaM and Mg2M. Keywords: exopolysaccharides, moderately halophilic bacteria, Halomonas INTRODUCTION In the course of our studies into hypersaline environ- ments, a group of Halomonas eurihalina strains were The family Halomonadaceae includes the genera isolated that synthesized large quantities of exopoly- Halomonas, Chromohalobacter and Zymobacter. -
2016 National Algal Biofuels Technology Review
National Algal Biofuels Technology Review Bioenergy Technologies Office June 2016 National Algal Biofuels Technology Review U.S. Department of Energy Office of Energy Efficiency and Renewable Energy Bioenergy Technologies Office June 2016 Review Editors: Amanda Barry,1,5 Alexis Wolfe,2 Christine English,3,5 Colleen Ruddick,4 and Devinn Lambert5 2010 National Algal Biofuels Technology Roadmap: eere.energy.gov/bioenergy/pdfs/algal_biofuels_roadmap.pdf A complete list of roadmap and review contributors is available in the appendix. Suggested Citation for this Review: DOE (U.S. Department of Energy). 2016. National Algal Biofuels Technology Review. U.S. Department of Energy, Office of Energy Efficiency and Renewable Energy, Bioenergy Technologies Office. Visit bioenergy.energy.gov for more information. 1 Los Alamos National Laboratory 2 Oak Ridge Institute for Science and Education 3 National Renewable Energy Laboratory 4 BCS, Incorporated 5 Bioenergy Technologies Office This report is being disseminated by the U.S. Department of Energy. As such, the document was prepared in compliance with Section 515 of the Treasury and General Government Appropriations Act for Fiscal Year 2001 (Public Law No. 106-554) and information quality guidelines issued by the Department of Energy. Further, this report could be “influential scientific information” as that term is defined in the Office of Management and Budget’s Information Quality Bulletin for Peer Review (Bulletin). This report has been peer reviewed pursuant to section II.2 of the Bulletin. Cover photo courtesy of Qualitas Health, Inc. BIOENERGY TECHNOLOGIES OFFICE Preface Thank you for your interest in the U.S. Department of Energy (DOE) Bioenergy Technologies Office’s (BETO’s) National Algal Biofuels Technology Review. -
Microbial Diversity of Soda Lake Habitats
Microbial Diversity of Soda Lake Habitats Von der Gemeinsamen Naturwissenschaftlichen Fakultät der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte D i s s e r t a t i o n von Susanne Baumgarte aus Fritzlar 1. Referent: Prof. Dr. K. N. Timmis 2. Referent: Prof. Dr. E. Stackebrandt eingereicht am: 26.08.2002 mündliche Prüfung (Disputation) am: 10.01.2003 2003 Vorveröffentlichungen der Dissertation Teilergebnisse aus dieser Arbeit wurden mit Genehmigung der Gemeinsamen Naturwissenschaftlichen Fakultät, vertreten durch den Mentor der Arbeit, in folgenden Beiträgen vorab veröffentlicht: Publikationen Baumgarte, S., Moore, E. R. & Tindall, B. J. (2001). Re-examining the 16S rDNA sequence of Halomonas salina. International Journal of Systematic and Evolutionary Microbiology 51: 51-53. Tagungsbeiträge Baumgarte, S., Mau, M., Bennasar, A., Moore, E. R., Tindall, B. J. & Timmis, K. N. (1999). Archaeal diversity in soda lake habitats. (Vortrag). Jahrestagung der VAAM, Göttingen. Baumgarte, S., Tindall, B. J., Mau, M., Bennasar, A., Timmis, K. N. & Moore, E. R. (1998). Bacterial and archaeal diversity in an African soda lake. (Poster). Körber Symposium on Molecular and Microsensor Studies of Microbial Communities, Bremen. II Contents 1. Introduction............................................................................................................... 1 1.1. The soda lake environment .................................................................................