4/6/2020 Sequencing Genomes - Genomes - NCBI Bookshelf
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Brown TA. Genomes. 2nd edition. Oxford: Wiley-Liss; 2002.
Chapter 6 Sequencing Genomes
Learning outcomes When you have read Chapter 6, you should be able to:
Distinguish between the two methods used to sequence DNA
Give a detailed description of chain termination sequencing and an outline description of the chemical degradation method
Describe the key features of automated DNA sequencing and evaluate the importance of automated sequencing in genomics research
State the strengths and limitations of the shotgun, whole-genome shotgun and clone contig methods of genome sequencing
Describe how a small bacterial genome can be sequenced by the shotgun method, using the Haemophilus influenzae project as an example
Outline the various ways in which a clone contig can be built up
Explain the basis to the whole-genome shotgun approach to genome sequencing, with emphasis on the steps taken to ensure that the resulting sequence is accurate
Give an account of the development of the Human Genome Project up to the publication of the draft sequence in February 2001
Debate the ethical, legal and social issues raised by the human genome projects