Wright's Stain Gurr®
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In Vitro Diagnostic Medical Device For professional use only Wright’s stain Gurr® for microscopical staining Cat. No Pack Type Pack Size Reagent 340804U Glass Bottle 25 g Cat. No Description Pack Size 34080 Wright’s Stain SS Gurr® 25g Composition 36311 Buffer tablets pH 6.8 1 pack (100 tabs) C.I. No. 52015 + Azure 0.8 g/l acc. to Weise C.I. No. 45380 0.8 g/l 1.09468 Buffer tablets acc. to Weise contains CH3OH pH 7,2 1 pack (100 tabs) Intended Use(s) Preparation Wright's stain is a histology stain that facilitates the 1. Buffer solution differentiation of blood cell types. It is primarily used for the Dissolve 1 buffer tablet acc. to Weise* in 1000 ml initial evaluation to differentiate nuclear and/or cytoplasmic distilled water. morphology of platelets, RBCs, WBCs for diagnosis, *1.09468 or 36311 depending on the required reaction (size,form and content) and examined under microscope . colour In cytogenetics it is used to stain chromosomes to facilitate 2. Wright’s eosin methylene blue solution diagnosis of syndromes and diseases. Dissolve 0.25 g Wright’s eosin methylene blue in 100 ml methanol, warm gently on a water bath for 20-30 min or Evaluate the result by comparing it to what would be the age until the powder is dissolved, filter before use. related normal values Review of the smears helps in determining the need for ancillary Sample material and preparation studies, such as cytochemistry, immunophenotyping, cytogenetic For professional use only. analysis, and molecular genetic study. Air-dried blood and bone marrow smears An initial review of the patient’s clinical background is Films are made by placing a drop of the samples on one end necessary to use in conjunction with the result of the staining of a slide, and using a spreader slide to disperse the sample over the slide's length. The aim is to get a region where the Samples derived from the human body cells are spaced far enough apart to be counted and differentiated.The slide is left to air dry Reference: In order to avoid errors, the staining process must be carried *A rapid method for the differential staining of blood films out by qualified personnel. Wright, J. H. A. National guidelines for work safety and quality assurance must be followed. Characteristics and performances Microscopes equipped according to the standard must be The typical color of cell nuclei, namely purple, is due to mole- used. cular interaction between eosin Y and an azure B-DNA complex Suitable instruments must be used for taking samples and for Wrights stain is a Romanowsky stain which includes a mixture their preparation. Follow the manufacturer’s instructions for of alkaline methylene blue and eosin. It differs from other application/use. Romanowsky stains in the way that the alkaline methylene blue (methylene azure) is produced and the relative All samples must be clearly labelled. proportions of the latter and eosin. All Romanowsky staining colour pictures are pH sensitive due Procedure to the methylene blue showing a greater intensity with Staining rack increasing alkalinity, whereas the eosin is more intense with Wright’s solution 1 min increasing acidity. To ensure a reproducible colour picture it is Buffer solution (1 ml) add, mix, stain 4 min essential therefore to work in a buffered environment. Of the Rinse with buffer solution two more common pH’s chosen 6.8 will favour a bluer picture Dry whereas 7.2 produces a stronger reddish colouring. VWR International bvba Researchpark Haasrode 2020 Geldenaaksebaan 464 3001 Leuven Belgium http://www.vwr.com Rev. Date: March 2011 Page 1 of 3 In Vitro Diagnostic Medical Device For professional use only Wright’s stain Gurr® for microscopical staining Staining cuvette Storage Wright’s solution 3 min Dilute Wright’s solution 6 min Store the dye at +5°C to +30°C. Rinse with buffer solution 2 x 1 min The solution and the dye must be used by Dry the expiry date stated. Staining with MIRASTAINER® (Automated instrument) Reagents Time Station DIP Wright’s solution 3 min 2 On Shelf life Dilute Wright’s solution 6 min 3 On After first opening the bottle, the contents Buffer solution 1 min 4 On can be used up to the expiry date when Running water (Rinse) 2 min 5 On stored at +15°C to +25°C (solution) resp. Dry 3 min 6 - +5°C to +30°C (dye). The bottles must be kept tightly closed at all times. Result The microscope used should meet the requirements of a medical diagnostic laboratory Nuclei red to violet Lymphocytes plasma blue Auxiliary reagents Monocytes plasma gray-blue Cat. No Description Pack Size Neutrophilic granulocytes granules light violet 36126 Microil Immersion Oil 100 ml Eosinophilic granulocytes granules brick-red to red-brown tropical grade Basophilic granulocytes granules dark violet to black 36104 Microil Immersion Oil 100 ml, 500 ml Thrombocytes violet 36102 Lenzol Immersion Oil Gurr 100 ml Erythrocytes reddish 36194 Fractoil Synthetic Immersion 500ml Oil Evaluate the result by comparing it to what would be the age 28975 Xylene Mixture of isomers 1 l, 2,5 l, 5 l, 25 l related normal values Normapur Anal Review of the smears helps in determining the need for 36125 DePeX® mounting medium 500ml ancillary studies, such as cytochemistry, immunophenotyping, 36029 DPX mountant 100ml, 500ml cytogenetic analysis, and molecular genetic study. An initial review of the patient’s clinical background is Precautioniary measures on health hazards necessary to use in conjuntion with the result of the staining Effective measures must be taken to protect against infection Samples derived from the human body in line with laboratory guidelines. Reference: Physical Hazard classification *A rapid method for the differential staining of blood films Please observe the hazard classification on the label and the Wright, J. H. A. information given in the safety data sheet. The VWR safety data sheet is available on the Internet. Diagnostics Instructions for environmental disposal Diagnoses are only to be made by authorised and trained Used solutions and solutions that are past their shelf-life must persons. Valid nomenclatures must be used. be disposed of as special waste according to local disposal Further tests must be selected and implemented according to guidelines.VWR International can provide technical support for recognised methods. local disposal solutions. VWR International bvba Researchpark Haasrode 2020 Geldenaaksebaan 464 3001 Leuven Belgium http://www.vwr.com Rev. Date: March 2011 Page 2 of 3 In Vitro Diagnostic Medical Device For professional use only Wright’s stain Gurr® for microscopical staining VWR International bvba Researchpark Haasrode 2020 Geldenaaksebaan 464 3001 Leuven Belgium http://www.vwr.com Rev. Date: March 2011 Page 3 of 3 .