Product datasheet Anti- beta 5/ITGB5 Antibody Catalog Number: A04201-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-/ITGB5 Antibody Name ITGB5 Source Rabbit IgG Species Reactivity human,mouse Tested Application WB,IHC-P,ICC/IF,FCM,Direct ELISA Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen E.coli-derived human beta 5/ITGB5 recombinant (Position: S32-D689). Purification Immunogen affinity purified. Observed MW 90KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry in paraffin section IHC-(P)： 1:50-400 Immunocytochemistry/Immunofluorescence (ICC/IF): 1:50-400 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information This gene encodes a beta subunit of integrin, which can combine with different alpha chains to form a variety of integrin heterodimers. are integral cell-surface receptors that participate in cell adhesion as well as cell-surface mediated signaling. The alphav beta5 integrin is involved in adhesion to vitronectin.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-Integrin beta 5/ITGB5 Antibody Catalog Number: A04201-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2.IHC analysis analysis of anti-ITGB5 using anti-ITGB5 antibody (A04201-1). The antibody (A04201-1). sample well of each lane detected in paraffin- was loaded with 50ug of embedded section of sample under reducing human rectal cancer conditions.Lane 1: human tissue. Biotinylated goat A549 whole cell anti-rabbit IgG was used lysates,Lane 2: Mouse as secondary antibody. Ana-1 whole cell The tissue section was lysates,Use rabbit anti- developed using Strepavi ITGB5 1:1000, probed din-Biotin-Complex with a goat anti-rabbit (SABC) (Catalog # IgG-HRP secondary SA1022) with DAB as the antibody. The signal is chromogen. developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ITGB5 at approximately 90KD. The expected band size for ITGB5 is at 90KD

Figure 3.ICC analysis Figure 4.Flow cytometry using anti-ITGB5 analysis of A549 antibody (A04201-1) was cell(1x106) DyLight 488 detected in immersion conjugated goat anti- fixed A431 cell line . Cells rabbit IgG(blue) was used were stained using the as secondary Dylight550-conjugated antibody.Isotype control Anti-rabbit IgG antibody (Green line) was Secondary Antibody rabbit IgG DyLight 488. (red)Catalog?#?BA1135) Unlabelled sample (Red and counterstained with line). DAPI (blue).

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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