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Influence of Meal Pattern and Dietary Fat on Cholesterol Metabolism in Adult Rats Recovering from Undernutrition Susan Anderson Carlson Iowa State University

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Influence of Meal Pattern and Dietary Fat on Cholesterol Metabolism in Adult Rats Recovering from Undernutrition Susan Anderson Carlson Iowa State University Iowa State University Capstones, Theses and Retrospective Theses and Dissertations Dissertations 1975 Influence of meal pattern and dietary fat on cholesterol metabolism in adult rats recovering from undernutrition Susan Anderson Carlson Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/rtd Part of the Dietetics and Clinical Nutrition Commons, Human and Clinical Nutrition Commons, and the Medical Nutrition Commons Recommended Citation Carlson, Susan Anderson, "Influence of meal pattern and dietary fat on cholesterol metabolism in adult rats recovering from undernutrition " (1975). Retrospective Theses and Dissertations. 5460. https://lib.dr.iastate.edu/rtd/5460 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Retrospective Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. INFORMATION TO USERS This material was produced from a microfilm copy of the original document. While the most advanced technological means to photograph and reproduce this document have been used, the quality is heavily dependent upon the quality of the original submitted. The following explanation of techniques is provided to help you understand markings or patterns which may appear on this reproduction. 1.The sign or "target" for pages apparently lacking from the document photographed is "Missing Page(s)". If it was possible to obtain the missing pane(s) or section, they are spliced into the film along with adjacent pages. This may have necessitated cutting thru an image and duplicating adjacent pages to insure you complete continuity. 2. When an image on the film is obliterated with a large round black mark, it is an indication that the photographer suspected that the copy may have moved during exposure and tiius cause a blurred image. You will find a good image of the page in the adjacent frame. 3. When a map, drawing or chart, etc., was part of the material being photographed the photographer followed a definite method In "sectioning" the material. It is customary to begin photoing at the upper Isft hsnd corncr cf 2 large ±est arid to Gon?m'.«« nhotoino (eft to right in equa! sections with a small overlap. !f necessary, sectioning is continued again — beginning below the first row and continuing on until complete. 4. The majority of users indicate that the textual content is of greatest value, however, a somewhat higher quality reproduction could be made from "photographs" if essential to the understanding of ths dissertation. Silver prints cf "photographs" may be ordered at additional charge by writing the Order Department, giving the catalog number, title, author and specific pages you wish reproduced. 5. PLEASE NOTE: Some pages may have indistinct print. Filmed as received. Xerox University Microfilms 300 North Zeeb Road Ann Arbor. Michigan 48106 I 76-1825 CARLSON, Susan Anderson, 1946- INFLUENCE OF MEAL PAHERN AND DIETARY FAT ON CHOLESTEROL METABOLISM IN ADULT RATS RECOVERING FROM UNDERNUTRITION. Iowa State University, Ph.D., 1975 Health Sciences, nutrition Xersx Ur.SverSSty Mjcrofllnis, Ann Arbor, Michigan 48106 THIS DISSERTATION HAS BEEN MICROFILMED EXACTLY AS RECEIVED. Influence of meal pattern and dietary fat on cholesterol metabolism in adult rats recovering from undernutrition by Susan Anderson Carlson A Dissertation Submitted to the Graduate Faculty in Partial Fulfillment of The Requirements for the Degree of DOCTOR OF PHILOSOPHY Department; Food and Nutrition Major: Nutrition Approved Signature was redacted for privacy. In Charge of Major Work Signature was redacted for privacy. For the Major Department Signature was redacted for privacy. Iowa State University Ames, Iowa 1975 ii TABLE OF CONTENTS Page GLOSSARY ix INTRODUCTION 1 LITERATURE REVIEW 4 Factors Contributing to Homeostasis in Cholesterol Metabolism 5 Influence of Dietary Variables on Cholesterol Metabolism 22 Influence of Periodicity of Eating on Choies- 33 Cerol Metabolism METHODS AND PROCEDURES 36 Selection and Treatment of Animals 36 Tissue Analysis 44 Statistical Analysis 56 RESULTS 57 Body Weights 58 Food Consumption and Feed Efficiency 58 Serum 5S Liver 67 Small Intestine 85 Epldidymal Fat Pad 91 Kidney 97 Heart 99 Feces 103 Carcass 109 ill Page DISCUSSION 114 Cholesterol Transport 117 Cholesterol Biosynthesis 127 Cholesterol (Re)absorption 131 Cholesterol Degradation 132 Carcass Composition 137 Llpogenesis 138 Summary 140 SIJUMAEY AND CONCLUSIONS 143 BIBLIOGRAPHY 148 ACKNOWLEDGMENTS 162 APPENDIX 163 iv LIST OF TABLES Stock ration for male rats: Modified Steenbock XVII 37 Composition of water soluble vitamin mixture 39 Composition of experimental diets 40 Experimental protocol 41 Serum and hepatic cholesterol concentrations (Experi­ ment 1) 60 Serum and hepatic cholesterol concentrations (Experi­ ment 2) 61 Serum and hepatic cholesterol concentrations (Experi­ ment 3) 62 Serum DPS, ^^C-DPS/ml and S.A. (^"^C-dpm x 10^/mg DPS) 65 Serum DPS, ^^C-DPS/ml and S.A. (^^C-dpm x 10®/mg DPS): Variable means and analysis of variance 66 Serum and hepatic cholesterol fatty acid patterns. Percent of total fatty acids 68 Hepatic weights and % lipid 70 Hepatic weights arid % lipid: Variable seaas and analy­ sis of variance 72 Hepatic ^H-DPS and ^H-DPS as % of total liver lipid : Variable means and analysis of variance 74 Hepatic DPS, ^^C-DPS and S.A. DPS ^"c-dpm x lOVmg) 76 Hepatic DPS, *^C-DPS and S.A. (^^C-dpm x 10^/sag DPS): Variable means and analysis of variance 77 Hepatic lipid, 'H-acetate incorporation and S.A. (^H-dpm X lO^/g lipid) 81 Hepatic lipid, ^H-acetate incorporation and S.A. (^H-dpm X 10*/g lipid): Variable sisans for analysis of variance 84 V Page Table 18. S.A. hepatic DPS (^"^C-dpm x 10^/mg) and ^ "^C-acld steroid 86 Table 19. Small intestine weight, DPS, ^^C-DPS, ^H-DPS and S.A. DPS (^^C-dprn x 10^ dpm/mg). Experi­ ments 2 and 3 87 Table 20. Small intestine conjugated bile acid - (Experiments 2 and 3) 90 Table 21. Carcass and epldidymal lipid deposition during refeeding (Experiment 1) 92 Table 22a. Epldidymal fat pad weight and lipid weight, *H-acetate incorporation and S.A. (^H-dpm x 10®/g). Experiment 2 95 Table 22b. Epldidymal lipid neutral-^ **0 (Experiment 2) 9.6 Table 23. Kidney weight. DPS, ^^C-DPS and S.A. DPS (^''C-dpm X loVmg). Experiment 2 98 Table 24. Heart weight, DPS, ^^C-DPS and S.A. DPS (i^C-dpm X 10^/mg). Experiment 2 100 Table 25. Fecal acid and neutral ^^C-steroid (Experiments 2 and 3 102 Table 26. Fecal acid and neutral ^^C-steroid: Variable means and analysis of variance 1 ny. Table 27. Acid and neutral **C-steroid excreted/g diet consumed 106 Table 28. Carcass DPS, total neutral ^^C-sterold and '"^C-DPS as proportion of total neutral ^^C-steroid (Experiment 2) 110 Table 29. Change in carcass composition with realimentation (Experiment 1) 112 Table 30. Neutral **C-sterold and specific activities DPS 123 Table 31. Estimate of total cholesterol present in serum and liver 124 Table 32. The Influence of fat source on hepatic and intestinal cholesterogenesis (Experiment 3) 126 vi Page Table 33, The influence of feeding frequency on hepatic and intestinal cholesterogenesis (Experiment 3) 126 Table 34. Body weights and days to depletion (Experiment 1) 164 Table 35. Body weights and days to depletion (Experiment 2) 165 Table 36. Body weights and days to depletion (Experiment 3) 166 Table 37. Food intakes and food efficiencies 167 Table 38. Food Intakes and food efficiencies; Variable means and analysis of variance 168 Table 39. Food efficiencies for refeeding period minus initial two days (Experiments 2 and 3) 169 vil LIST OF FIGURES Page Figure 1. Conversion of acetate to cholesterol 6 Figure 2. Schematic of liver analyses 4 7 Figure 3. Hepatic lipid - ^^C 6 days following intraperi­ toneal injection of 5 ]iC 4-^'^C-cholesterol; total hepatic lipid and DPS (digltonin precipi- table sterol). Experiment 2 79 Figure 4. Hepatic lipid - 'h 2.5 hours following injection with 50 uC ®H-acetate. Total hepatic lipid and DPS (digltonin preclpltable sterol). Experiment 2 83 Figure 5. Weight carcass fat compared with weight epididymal fat (Experiment 1) 94b Figure 6. Fecal acid and neutral steroid (% of total excreted steroid ^^C-dpm). Experiments 2 and 3 105 Figure 7. Fecal acid and neutral steroid (^*C-dpm/g diet consumed). Experiment 3 107 Figure 8. Comparison of serum and hepatic cholesterol concen­ trations by meal pattern (AL,I-1F). Ehcperiment 2 119 Figure 9. Comparison of serum and hepatic cholesterol concen­ trations by protein (P,4P). Experiment 3 i20 Figure 10. Food consumption in kcal/2 days of the 10 day reall- mentation period. Food consumption plotted by diet (Stocks OF-Ff 20S0-P. 2CBT=P) and meal pattern (AL, MF). Experiment 2 171 Figure 11= Food efficiency (g gain/100 kcâl)/2 days of the 10 day reallmentation period. Food efficiency plotted by diet (Stock, OF-P, 2050-?, 205T-P) and meal pattern (AL, MF): Experiment: 2 173 Figure 12, Food consumption in kcal/2 days of the 10 day reali­ mentation period. Food consumption plotted by diet (20S0-P, 20S0-4P, 20BT-P, 20BT-4P) and meal pattern (AL, MF). Experiment 3 175 viii Page Figure 13. Food efficiency (g gain/100 kcal)/2 days of the 10 day reallmentation period. Food efficiency plotted by diet (20S0-P, 20S0-4P, 20BT-P, 20BT-4P) and meal pattern (AL, MF). Experiment 3 177 ix GLOSSARY OF,OP - contains
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