TBX3 Acts As Tissue-Specific Component of the Wnt/Β
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Constitutive Scaffolding of Multiple Wnt Enhanceosome Components By
RESEARCH ARTICLE Constitutive scaffolding of multiple Wnt enhanceosome components by Legless/ BCL9 Laurens M van Tienen, Juliusz Mieszczanek, Marc Fiedler, Trevor J Rutherford, Mariann Bienz* MRC Laboratory of Molecular Biology, Cambridge, United Kingdom Abstract Wnt/b-catenin signaling elicits context-dependent transcription switches that determine normal development and oncogenesis. These are mediated by the Wnt enhanceosome, a multiprotein complex binding to the Pygo chromatin reader and acting through TCF/LEF- responsive enhancers. Pygo renders this complex Wnt-responsive, by capturing b-catenin via the Legless/BCL9 adaptor. We used CRISPR/Cas9 genome engineering of Drosophila legless (lgs) and human BCL9 and B9L to show that the C-terminus downstream of their adaptor elements is crucial for Wnt responses. BioID proximity labeling revealed that BCL9 and B9L, like PYGO2, are constitutive components of the Wnt enhanceosome. Wnt-dependent docking of b-catenin to the enhanceosome apparently causes a rearrangement that apposes the BCL9/B9L C-terminus to TCF. This C-terminus binds to the Groucho/TLE co-repressor, and also to the Chip/LDB1-SSDP enhanceosome core complex via an evolutionary conserved element. An unexpected link between BCL9/B9L, PYGO2 and nuclear co-receptor complexes suggests that these b-catenin co-factors may coordinate Wnt and nuclear hormone responses. DOI: 10.7554/eLife.20882.001 *For correspondence: mb2@mrc- Introduction lmb.cam.ac.uk The Wnt/b-catenin signaling cascade is an ancient cell communication pathway that operates con- Competing interests: The text-dependent transcriptional switches to control animal development and tissue homeostasis authors declare that no (Cadigan and Nusse, 1997). -
Wnt Signaling in Vertebrate Neural Development and Function
J Neuroimmune Pharmacol (2012) 7:774–787 DOI 10.1007/s11481-012-9404-x INVITED REVIEW Wnt Signaling in Vertebrate Neural Development and Function Kimberly A. Mulligan & Benjamin N. R. Cheyette Received: 18 June 2012 /Accepted: 10 September 2012 /Published online: 27 September 2012 # Springer Science+Business Media, LLC 2012 Abstract Members of the Wnt family of secreted signaling immature neurons migrate to populate different cortical proteins influence many aspects of neural development and layers, nuclei, and ganglia in the forebrain, midbrain, hind- function. Wnts are required from neural induction and axis brain, and spinal cord. Each mature neuron elaborates an formation to axon guidance and synapse development, and axon and numerous dendrites while forming hundreds to even help modulate synapse activity. Wnt proteins activate a thousands of synapses with other neurons, enabling electro- variety of downstream signaling pathways and can induce a chemical communication throughout the nervous system. similar variety of cellular responses, including gene tran- All these developmental processes must be coordinated to scription changes and cytoskeletal rearrangements. This re- ensure proper construction and function of the CNS, and view provides an introduction to Wnt signaling pathways this requires the coordinated developmental activity of a and discusses current research on their roles in vertebrate vast array of genes. neural development and function. Members of the Wnt family of secreted signaling proteins are implicated in every step of neural development men- Keywords Wnt signaling . Neural development . tioned above. Wnt proteins provide positional information CNS patterning . Axon guidance . Dendrite growth . within the embryo for anterior-posterior axis specification of Synapse formation . -
Supplemental Materials ZNF281 Enhances Cardiac Reprogramming
Supplemental Materials ZNF281 enhances cardiac reprogramming by modulating cardiac and inflammatory gene expression Huanyu Zhou, Maria Gabriela Morales, Hisayuki Hashimoto, Matthew E. Dickson, Kunhua Song, Wenduo Ye, Min S. Kim, Hanspeter Niederstrasser, Zhaoning Wang, Beibei Chen, Bruce A. Posner, Rhonda Bassel-Duby and Eric N. Olson Supplemental Table 1; related to Figure 1. Supplemental Table 2; related to Figure 1. Supplemental Table 3; related to the “quantitative mRNA measurement” in Materials and Methods section. Supplemental Table 4; related to the “ChIP-seq, gene ontology and pathway analysis” and “RNA-seq” and gene ontology analysis” in Materials and Methods section. Supplemental Figure S1; related to Figure 1. Supplemental Figure S2; related to Figure 2. Supplemental Figure S3; related to Figure 3. Supplemental Figure S4; related to Figure 4. Supplemental Figure S5; related to Figure 6. Supplemental Table S1. Genes included in human retroviral ORF cDNA library. Gene Gene Gene Gene Gene Gene Gene Gene Symbol Symbol Symbol Symbol Symbol Symbol Symbol Symbol AATF BMP8A CEBPE CTNNB1 ESR2 GDF3 HOXA5 IL17D ADIPOQ BRPF1 CEBPG CUX1 ESRRA GDF6 HOXA6 IL17F ADNP BRPF3 CERS1 CX3CL1 ETS1 GIN1 HOXA7 IL18 AEBP1 BUD31 CERS2 CXCL10 ETS2 GLIS3 HOXB1 IL19 AFF4 C17ORF77 CERS4 CXCL11 ETV3 GMEB1 HOXB13 IL1A AHR C1QTNF4 CFL2 CXCL12 ETV7 GPBP1 HOXB5 IL1B AIMP1 C21ORF66 CHIA CXCL13 FAM3B GPER HOXB6 IL1F3 ALS2CR8 CBFA2T2 CIR1 CXCL14 FAM3D GPI HOXB7 IL1F5 ALX1 CBFA2T3 CITED1 CXCL16 FASLG GREM1 HOXB9 IL1F6 ARGFX CBFB CITED2 CXCL3 FBLN1 GREM2 HOXC4 IL1F7 -
Nucleo-Cytoplasmic Distribution of ß-Catenin Is Regulated by Retention
Research Article 1453 Nucleo-cytoplasmic distribution of -catenin is regulated by retention Eva Krieghoff, Jürgen Behrens* and Bernhard Mayr Nikolaus-Fiebiger-Center for Molecular Medicine, University of Erlangen-Nürnberg, Glückstr. 6, 91054 Erlangen, Germany *Author for correspondence (e-mail: [email protected]) Accepted 19 December 2005 Journal of Cell Science 119, 1453-1463 Published by The Company of Biologists 2006 doi:10.1242/jcs.02864 Summary -catenin is the central signalling molecule of the canonical of -catenin, i.e. increases the rate of -catenin nuclear Wnt pathway, where it activates target genes in a complex import or export. Moreover, the cytoplasmic enrichment of with LEF/TCF transcription factors in the nucleus. The -catenin by APC and axin is not abolished by inhibition regulation of -catenin activity is thought to occur mainly of CRM-1-dependent nuclear export. TCF4, APC, axin and on the level of protein degradation, but it has been axin2 move more slowly than -catenin in their respective suggested that -catenin nuclear localization and hence its compartment, and concomitantly decrease -catenin transcriptional activity may additionally be regulated via mobility. Together, these data indicate that -catenin nuclear import by TCF4 and BCL9 and via nuclear export interaction partners mainly regulate -catenin subcellular by APC and axin. Using live-cell microscopy and localization by retaining it in the compartment in which fluorescence recovery after photobleaching (FRAP), we they are localized, rather than by active transport into or have directly analysed the impact of these factors on the out of the nucleus. subcellular localization of -catenin, its nucleo-cytoplasmic shuttling and its mobility within the nucleus and the Supplementary material available online at cytoplasm. -
DIXDC1 Contributes to Psychiatric Susceptibility by Regulating Dendritic Spine and Glutamatergic Synapse Density Via GSK3 and Wnt/Β-Catenin Signaling
DIXDC1 contributes to psychiatric susceptibility by regulating dendritic spine and glutamatergic synapse density via GSK3 and Wnt/β-catenin signaling The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Martin, P., R. E. Stanley, A. P. Ross, A. E. Freitas, C. E. Moyer, A. C. Brumback, J. Iafrati, et al. 2016. “DIXDC1 contributes to psychiatric susceptibility by regulating dendritic spine and glutamatergic synapse density via GSK3 and Wnt/β-catenin signaling.” Molecular psychiatry :10.1038/mp.2016.184. doi:10.1038/mp.2016.184. http:// dx.doi.org/10.1038/mp.2016.184. Published Version doi:10.1038/mp.2016.184 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:32630523 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA HHS Public Access Author manuscript Author ManuscriptAuthor Manuscript Author Mol Psychiatry Manuscript Author . Author Manuscript Author manuscript; available in PMC 2017 April 19. DIXDC1 contributes to psychiatric susceptibility by regulating dendritic spine and glutamatergic synapse density via GSK3 and Wnt/β-catenin signaling Pierre-Marie Martin#1, Robert E. Stanley#1,2, Adam P. Ross#1, Andiara E. Freitas#1, Caitlin E. Moyer3, Audrey C. Brumback1,4, Jillian Iafrati1, Kristina S. Stapornwongkul1, Sky Dominguez1, Saul Kivimäe1, Kimberly A. Mulligan1,5, Mehdi Pirooznia6, W. Richard McCombie7, James B. Potash8, Peter P. Zandi9, Shaun M. -
Tbx3 Controls the Sinoatrial Node Gene Program and Imposes Pacemaker Function on the Atria
Downloaded from genesdev.cshlp.org on September 29, 2021 - Published by Cold Spring Harbor Laboratory Press Tbx3 controls the sinoatrial node gene program and imposes pacemaker function on the atria Willem M.H. Hoogaars,1,3 Angela Engel,2,3 Janynke F. Brons,1,3 Arie O. Verkerk,2 Frederik J. de Lange,1 L.Y. Elaine Wong,1 Martijn L. Bakker,1 Danielle E. Clout,1 Vincent Wakker,1 Phil Barnett,1 Jan Hindrik Ravesloot,2 Antoon F.M. Moorman,1 E. Etienne Verheijck,2 and Vincent M. Christoffels1,4 1Department of Anatomy and Embryology, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands; 2Department of Physiology, Heart Failure Research Center, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands The sinoatrial node initiates the heartbeat and controls the rate and rhythm of contraction, thus serving as the pacemaker of the heart. Despite the crucial role of the sinoatrial node in heart function, the mechanisms that underlie its specification and formation are not known. Tbx3, a transcriptional repressor required for development of vertebrates, is expressed in the developing conduction system. Here we show that Tbx3 expression delineates the sinoatrial node region, which runs a gene expression program that is distinct from that of the bordering atrial cells. We found lineage segregation of Tbx3-negative atrial and Tbx3-positive sinoatrial node precursor cells as soon as cardiac cells turn on the atrial gene expression program. Tbx3 deficiency resulted in expansion of expression of the atrial gene program into the sinoatrial node domain, and partial loss of sinoatrial node-specific gene expression. -
BCL9 Provides Multi-Cellular Communication Properties in Colorectal Cancer by Interacting with Paraspeckle Proteins
ARTICLE https://doi.org/10.1038/s41467-019-13842-7 OPEN BCL9 provides multi-cellular communication properties in colorectal cancer by interacting with paraspeckle proteins Meng Jiang 1,2,8, Yue Kang1,3,8, Tomasz Sewastianik1,4, Jiao Wang1,5, Helen Tanton1, Keith Alder1, Peter Dennis1, Yu Xin1, Zhongqiu Wang1,6, Ruiyang Liu1, Mengyun Zhang1, Ying Huang1, Massimo Loda1, Amitabh Srivastava7, Runsheng Chen3, Ming Liu2 & Ruben D. Carrasco1,7* 1234567890():,; Colorectal cancer (CRC) is the third most commonly diagnosed cancer, which despite recent advances in treatment, remains incurable due to molecular heterogeneity of tumor cells. The B-cell lymphoma 9 (BCL9) oncogene functions as a transcriptional co-activator of the Wnt/ β-catenin pathway, which plays critical roles in CRC pathogenesis. Here we have identified a β-catenin-independent function of BCL9 in a poor-prognosis subtype of CRC tumors char- acterized by expression of stromal and neural associated genes. In response to spontaneous calcium transients or cellular stress, BCL9 is recruited adjacent to the interchromosomal regions, where it stabilizes the mRNA of calcium signaling and neural associated genes by interacting with paraspeckle proteins. BCL9 subsequently promotes tumor progression and remodeling of the tumor microenvironment (TME) by sustaining the calcium transients and neurotransmitter-dependent communication among CRC cells. These data provide additional insights into the role of BCL9 in tumor pathogenesis and point towards additional avenues for therapeutic intervention. 1 Department of Oncologic Pathology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA. 2 Department of General Surgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin Medical University, Harbin 150001, China. -
PIP3-Phldb2 Is Crucial for LTP Regulating Synaptic NMDA and AMPA Receptor Density and PSD95 Turnover
国立大学法人電気通信大学 / The University of Electro-Communications PIP3-Phldb2 is crucial for LTP regulating synaptic NMDA and AMPA receptor density and PSD95 turnover 著者(英) Min-Jue Xie, Yasuyuki Ishikawa, Hideshi Yagi, Tokuichi Iguchi, Yuichiro Oka, Kazuki Kuroda, Keiko Iwata, Hiroshi Kiyonari, Shinji Matsuda, Hideo Matsuzaki, Michisuke Yuzaki, Yugo Fukazawa, Makoto Sato journal or Scientific Reports publication title volume 9 number 1 page range 4305 year 2019-03-13 URL http://id.nii.ac.jp/1438/00009269/ doi: 10.1038/s41598-019-40838-6 Creative Commons Attribution 4.0 International License www.nature.com/scientificreports OPEN PIP3-Phldb2 is crucial for LTP regulating synaptic NMDA and AMPA receptor density and PSD95 Received: 1 November 2018 Accepted: 11 February 2019 turnover Published: xx xx xxxx Min-Jue Xie1,2,3,4,5, Yasuyuki Ishikawa6,7, Hideshi Yagi1,8, Tokuichi Iguchi1,9, Yuichiro Oka1,5,9, Kazuki Kuroda1,2,4, Keiko Iwata3,4,5, Hiroshi Kiyonari10, Shinji Matsuda11,12,13, Hideo Matsuzaki3,5, Michisuke Yuzaki 11, Yugo Fukazawa 2,3,4 & Makoto Sato 1,3,5,9 The essential involvement of phosphoinositides in synaptic plasticity is well-established, but incomplete knowledge of the downstream molecular entities prevents us from understanding their signalling cascades completely. Here, we determined that Phldb2, of which pleckstrin-homology domain is highly sensitive to PIP3, functions as a phosphoinositide-signalling mediator for synaptic plasticity. BDNF application caused Phldb2 recruitment toward postsynaptic membrane in dendritic spines, whereas PI3K inhibition resulted in its reduced accumulation. Phldb2 bound to postsynaptic scafolding molecule PSD-95 and was crucial for localization and turnover of PSD-95 in the spine. -
The Molecular Design of Visual Transduction
The Molecular Design of Visual Transduction Tomoki Isayama1, Anita L. Zimmerman2, Clint L. Makino1 1Department of Ophthalmology, Massachusetts Eye and Ear Infirmary and Harvard Medical School, Boston, MA 02114; 2Department of Molecular Pharmacology, Physiology, and Biotechnology, Brown University, Providence, RI 02912 Our daily lives are so resplendent with activities that include or require vision, that it is easy to overlook the complexity of the underlying processes involved. Here we describe the first step in vision…detection of light by our photoreceptors. This input stage sets limits on what we can and cannot see. The goal of this resource page is to impart a better understanding of the molecular design behind visual transduction. Structure and Function of Rods and Cones The photoreceptors are located in the deepest layer of the retina (Fig.1), farthest from the incoming light. There are two kinds, rods and cones, so named for their overall shapes. Rods operate in very low light, such as at night. Cones operate under brighter conditions and provide the basic units for color vision. The recently discovered intrinsically photoreceptive ganglion cells will be excluded from this review because while they respond to light, they have not been shown to contribute to image-forming vision. Rods and cones are specialized unipolar neurons. All vertebrate visual receptors follow a simple blueprint. They can be divided into two portions, termed inner and outer segments, according to their radial position within the retina (Fig.1B). The inner segment consists of the cell body and contains the cellular organelles found in other neurons, including a synaptic terminal. -
The Role of Tbx5 in Sinoatrial Node Differentiation in Mouse Embryonic Stem Cell Derived Cardiomyocytes Yunkai Dai Clemson University, [email protected]
Clemson University TigerPrints All Dissertations Dissertations May 2019 The Role of Tbx5 in Sinoatrial Node Differentiation in Mouse Embryonic Stem Cell Derived Cardiomyocytes Yunkai Dai Clemson University, [email protected] Follow this and additional works at: https://tigerprints.clemson.edu/all_dissertations Recommended Citation Dai, Yunkai, "The Role of Tbx5 in Sinoatrial Node Differentiation in Mouse Embryonic Stem Cell Derived Cardiomyocytes" (2019). All Dissertations. 2377. https://tigerprints.clemson.edu/all_dissertations/2377 This Dissertation is brought to you for free and open access by the Dissertations at TigerPrints. It has been accepted for inclusion in All Dissertations by an authorized administrator of TigerPrints. For more information, please contact [email protected]. THE ROLE OF TBX5 IN SINOATRIAL NODE DIFFERENTIATION IN MOUSE EMBRYONIC STEM CELL DERIVED CARDIOMYOCYTES A Dissertation Presented to the Graduate School of Clemson University In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy Bioengineering by Yunkai Dai May 2019 Accepted by: Dr Ann Foley, Committee Chair Dr Agneta Simionescu Dr Robin C. Muise-Helmericks Dr Ying Mei ABSTRACT The sinoatrial node of the mouse embryo arises from the wall of the right atrium near the border of the sinus venosus. Early in development this region expresses the transcription factor Tbx5. Because of this, Tbx5 is thought to sit at the apex of a transcriptional cascade leading to sinoatrial node (SAN) differentiation. To test this we produced a mouse embryonic stem cell line B1 (pTripZ-mTbx5; αMHC::GFP) that conditionally overexpresses Tbx5, to determine if this would lead to enhanced SAN differentiation. We found that ES cells overexpressing Tbx5 showed enhanced overall cardiac differentiation and that cardiac cells showed increased beat rates as compared control embryos. -
Differential Spatio-Temporal Regulation of T-Box Gene Expression by Micrornas During Cardiac Development
Journal of Cardiovascular Development and Disease Article Differential Spatio-Temporal Regulation of T-Box Gene Expression by microRNAs during Cardiac Development Mohamad Alzein, Estefanía Lozano-Velasco , Francisco Hernández-Torres , Carlos García-Padilla , Jorge N. Domínguez , Amelia Aránega and Diego Franco * Cardiovascular Development Group, Department of Experimental Biology, University of Jaen, 23071 Jaen, Spain; [email protected] (M.A.); [email protected] (E.L.-V.); [email protected] (F.H.-T.); [email protected] (C.G.-P.); [email protected] (J.N.D.); [email protected] (A.A.) * Correspondence: [email protected] Abstract: Cardiovascular development is a complex process that starts with the formation of symmet- rically located precardiac mesodermal precursors soon after gastrulation and is completed with the formation of a four-chambered heart with distinct inlet and outlet connections. Multiple transcrip- tional inputs are required to provide adequate regional identity to the forming atrial and ventricular chambers as well as their flanking regions; i.e., inflow tract, atrioventricular canal, and outflow tract. In this context, regional chamber identity is widely governed by regional activation of distinct T-box family members. Over the last decade, novel layers of gene regulatory mechanisms have been discovered with the identification of non-coding RNAs. microRNAs represent the most well-studied subcategory among short non-coding RNAs. In this study, we sought to investigate the functional role of distinct microRNAs that are predicted to target T-box family members. Our data demonstrated a highly dynamic expression of distinct microRNAs and T-box family members during cardiogenesis, revealing a relatively large subset of complementary and similar microRNA–mRNA expression pro- Citation: Alzein, M.; Lozano-Velasco, files. -
Whole-Exome Sequencing of Metastatic Cancer and Biomarkers of Treatment Response
Supplementary Online Content Beltran H, Eng K, Mosquera JM, et al. Whole-exome sequencing of metastatic cancer and biomarkers of treatment response. JAMA Oncol. Published online May 28, 2015. doi:10.1001/jamaoncol.2015.1313 eMethods eFigure 1. A schematic of the IPM Computational Pipeline eFigure 2. Tumor purity analysis eFigure 3. Tumor purity estimates from Pathology team versus computationally (CLONET) estimated tumor purities values for frozen tumor specimens (Spearman correlation 0.2765327, p- value = 0.03561) eFigure 4. Sequencing metrics Fresh/frozen vs. FFPE tissue eFigure 5. Somatic copy number alteration profiles by tumor type at cytogenetic map location resolution; for each cytogenetic map location the mean genes aberration frequency is reported eFigure 6. The 20 most frequently aberrant genes with respect to copy number gains/losses detected per tumor type eFigure 7. Top 50 genes with focal and large scale copy number gains (A) and losses (B) across the cohort eFigure 8. Summary of total number of copy number alterations across PM tumors eFigure 9. An example of tumor evolution looking at serial biopsies from PM222, a patient with metastatic bladder carcinoma eFigure 10. PM12 somatic mutations by coverage and allele frequency (A) and (B) mutation correlation between primary (y- axis) and brain metastasis (x-axis) eFigure 11. Point mutations across 5 metastatic sites of a 55 year old patient with metastatic prostate cancer at time of rapid autopsy eFigure 12. CT scans from patient PM137, a patient with recurrent platinum refractory metastatic urothelial carcinoma eFigure 13. Tracking tumor genomics between primary and metastatic samples from patient PM12 eFigure 14.