1 Advances in the systematics of the spider genus Troglohyphantes (Araneae, 2 Linyphiidae) 3 4 Marco Isaia1 *, Stefano Mammola1, Paola Mazzuca2, Miquel A. Arnedo2 & Paolo Pantini3 5 6 1) Department of Life Sciences and Systems Biology, Università di Torino. Via Accademia 7 Albertina, 13. I-10123 Torino, Italy. 8 2) Department of Evolutionary Biology, Ecology and Environmental Sciences & Biodiversity 9 Research Institute, Universitat de Barcelona. Av. Diagonal 643, Barcelona 08028, Catalonia, Spain. 10 3) Museo civico di Scienze Naturali “E. Caffi”. Piazza Cittadella, 10. I-24129 Bergamo, Italy. 11 * Corresponding author:
[email protected] 12 13 Running title: Advances in Troglohyphantes systematics 14 15 16 17 18 19 20 21 22 ABSTRACT 23 With 128 described species and 5 subspecies, the spider genus Troglohyphantes (Araneae, 24 Linyphiidae) is a remarkable example of species diversification in the subterranean environment. In 25 this paper, we conducted a systematic revision of the Troglohyphantes species of the Italian Alps, 26 with a special focus on the Lucifuga complex, including the description of two new species (T. 27 lucifer n. sp. and T. apenninicus n. sp). In addition, we provided new diagnostic drawings of the 28 holotype of T. henroti (Henroti complex) and established three new synonymies within the genus. 29 The molecular analysis of the animal DNA barcode confirms the validity of this method of 30 identification of the Alpine Troglohyphantes and provides additional support for the morphology- 31 based species complexes. Finally, we revised the known distribution range of additional 32 Troglohyphantes species, as well as other poorly known alpine cave-dwelling spiders.