Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

1.1 Distribution List Tammy Riddell Project Manager WA State Department of Ecology PO Box 47775 Olympia, WA 98504-7775 (360) 407-6295 [email protected] Michael Dawson Water Quality Manager Jefferson County Public Health 615 Sheridan St Port Townsend, WA 98368 (360) 385-9444 [email protected] Nancy Parrott Laboratory Supervisor/QA Officer Spectra Laboratories – Kitsap, LLC (Formerly Twiss Laboratories, Inc.) 26276 Twelve Trees Lane, Suite C Poulsbo, WA 98370 (360) 779-5141 [email protected]

Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Table of Contents 1.0 Title Page/Approvals ...... 1 1.1 Distribution List ...... 2 2.0 Abstract ...... 7 3.0 Background ...... 7 3.1 Study Area and Surroundings ...... 7 3.1.1 Logistical Problems ...... 7 3.1.2 History of Study Area ...... 8 3.1.3 Contaminants of Concern ...... 9 3.1.4 Results of Previous Studies ...... 10 3.1.5 Regulatory Criteria or Standards ...... 10 4.0 Project Description ...... 10 4.1 Project Goals ...... 10 4.2 Project Objectives ...... 11 4.3 Information Needed and Sources ...... 11 4.4 Target Population...... 11 4.5 Study Boundaries ...... 11 4.6 Tasks Required ...... 12 4.7 Practical Constraints ...... 13 4.8 Systematic Planning Process ...... 13 5.0 Organization and Schedule ...... 13 5.1 Key Individuals and Their Responsibilities ...... 13 5.2 Special Training and Certifications ...... 13 5.3 Organization Chart ...... 14 5.4 Project Schedule ...... 14 5.5 Limitations on Schedule ...... 14 5.6 Budget and Funding ...... 14 6.0 Quality Objectives ...... 14 6.1 Decision Quality Objectives (DQOs) ...... 14 6.2 Measurement Quality Objectives (MQOs) ...... 14 6.2.1 Targets for Precision, Bias, and Sensitivity ...... 15 6.2.2 Targets for Comparability, Representativeness, & Completeness ...... 16 7.0 Sampling Process Design ...... 16 7.1 Study Design...... 16 7.1.1 Sampling Locations and Frequency ...... 17 Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

7.1.2 Parameters to be Determined ...... 18 7.1.3 Field Measurements ...... 18 7.2 Maps or Diagrams ...... 19 7.3 Assumptions Underlying Design ...... 19 7.4 Relation to Objectives and Site Characteristics ...... 19 7.5 Characteristics of Existing Data ...... 19 8.0 Sampling Procedures ...... 19 8.1 Field Sampling SOPs ...... 19 8.2 Containers, Preservation, Holding Times ...... 20 8.3 Invasive Species Evaluation...... 20 8.4 Equipment Decontamination ...... 20 8.5 Sample ID ...... 20 8.6 Chain-of-Custody ...... 21 8.7 Field Log Requirements...... 21 9.0 Measurement Methods ...... 21 9.1 Field Procedures/Field Analysis ...... 21 9.2 Laboratory Procedures...... 22 9.2.1 Analytes ...... 23 9.2.2 Matrix ...... 23 9.2.3 Number of Samples ...... 23 9.2.4 Expected Range of Results ...... 23 9.2.5 Analytical Methods ...... 23 9.2.6 Sensitivity/Method Detection Limit (MDL) ...... 23 9.3 Sample Preparation Method ...... 23 9.4 Special Method Requirements...... 23 9.5 Lab(s) Accredited for Methods ...... 23 10.0 Quality Control ...... 24 10.1 Table of Lab and Field QC Required ...... 24 10.2 Corrective Action ...... 24 11.0 Data Management Procedures ...... 24 11.1 Data Recording/Reporting Requirements ...... 24 11.2 Lab Data Package Requirements ...... 24 11.3 Electronic Transfer Requirements ...... 24 11.4 Acceptance Criteria for Existing Data ...... 25 11.5 EIM/STORET Data Upload Procedures ...... 25 Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

12.0 Audits and Reports ...... 25 12.1 Audit Number, Frequency, Type and Schedule ...... 25 12.2 Responsible Personnel ...... 25 12.3 Frequency and Distribution of Reports ...... 25 12.4 Responsibility for Reports ...... 25 13.0 Data Verification ...... 25 13.1 Field Data Verification, Requirements, and Responsibilities ...... 25 13.2 Lab Data Verification ...... 25 13.3 Validation Requirements ...... 26 14.0 Data Quality (Usability) Assessment ...... 26 14.1 Process for Determining Whether Project Objectives Have Been Met ...... 26 14.2 Data Analysis and Presentation Methods ...... 26 14.3 Treatment of Non-Detects ...... 26 14.4 Sampling Design Evaluation ...... 26 14.5 Documentation of Assessment ...... 26 15.0 References ...... 27 16.0 Figures ...... 29 17.0 Tables ...... 36 18.0 Appendices ...... 53 A. Chain-of-Custody Form ...... 53 B. Glossary, Acronyms and Abbreviations, Units of Measurement ...... 54 Quality Assurance Glossary ...... 54 Acronyms and Abbreviations ...... 57 Units of Measurement ...... 58

Figures Figure 1, Study Area ...... 29 Figure 2, 2000 aerial photo of Brinnon ...... 30 Figure 3, 2013 aerial photo of Brinnon ...... 30 Figure 4, E. coli & Sanitary Survey Results, 2007-2014 ...... 31 Figure 5, Project Organizational Chart ...... 32 Figure 6, MQO Analysis Process for Bacteria (Mathieu, 2006) ...... 32 Figure 7, MQO Analysis Process for all Parameters, Excluding Bacteria (Mathieu, 2006) ...... 33

Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Figure 8, Hotspot Confirmation ...... 34 Figure 9, Shoreline & Marine Monitoring Stations, Quilcene-Dabob Bay ...... 35

Tables Table 1, Measurement Quality Objectives ...... 36 Table 2, Sample Containers, Preservation, and Holding Times ...... 36 Table 3, Measurement Methods ...... 37 Table 4, QC Samples, Types and Frequency ...... 38 Table 5, Hood Canal Watershed Phase 1 2011-12, Water Quality Standards Pass/Fail ...... 38 Table 6, Hood Canal Clean Streams Dec 2014-Jan 2016, Water Quality Standards Pass/Fail ...... 38 Table 7, Dept. of Ecology Regional Stormwater Monitoring Data, Jan-Nov 2015 ...... 39 Table 8, BEACH Monitoring Data, 2004-2014 ...... 39 Table 9, Water Quality Standards for Fecal Coliform ...... 39 Table 10, Project Schedule ...... 40 Table 11, Budget by Task ...... 41 Table 12, Geographic Data Parameters ...... 41 Table 13, Hotspot Investigation ...... 41 Table 14, Shoreline Monitoring Stations, Quilcene-Dabob Bay ...... 43 Table 15, Stream Monitoring Stations, Quilcene-Dabob Bay ...... 50 Table 16, Stream Monitoring Stations, Quilcene-Dabob Bay ...... 51 Table 17, Sample Frequency and Number of Samples ...... 51 Table 18, Expected Range of Results ...... 52 Table 19, Reporting Requirements ...... 52 Table 20, Entering Non-detects and TNTC data in EIM ...... 52

Appendices A, Chain-of-Custody Form ...... 53 B, Glossary, Acronyms, and Abbreviations ...... 54

Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

2.0 Abstract The Quilcene-Dabob Pollution Identification and Correction (PIC) project will address 50 miles of coastline and 17,000 acres of marine water. Jefferson County Public Health (JCPH) plans to conduct four marine/shoreline surveys in the project area, two during wet season (October - April), and two during dry season (May - September). During the shoreline survey, JCPH will sample 16 marine stations and all freshwater inputs for fecal coliform bacteria and nutrients. Additionally, JCPH will conduct one year of stream monitoring at 17 stations for fecal coliform bacteria. JCPH will identify areas of concern based on sampling results and take additional samples to locate possible onsite septic system (OSS) failure, or other pollutant sources. The goal of the project is to protect human health from risks of waterborne pathogens and keep shellfish beds open and water safe for recreation.

3.0 Background 3.1 Study Area and Surroundings The project area includes Quilcene Bay and Dabob Bay, which are located in Water Resource Inventory Area (WRIA) 17 on the eastern edge of the Olympic Peninsula in eastern Jefferson County, Washington (see Figure 1, Study Area). Quilcene Bay and Dabob Bay receive freshwater inputs from the major river valleys of the Dosewallips, Big Quilcene, and Little Quilcene rivers, and from smaller streams such as Spencer, Marple, Donovan, and Tarboo Creeks. The communities of Brinnon and Quilcene are located along the western shore of the project area. The 2010 Census listed the population of Brinnon at 797, and Quilcene School District at 596.

The headwaters of Dosewallips River and Big Quilcene River originate in Olympic National Park (ONP) and the Buckhorn Wilderness area of Olympic National Forest (ONF), respectively. Other forest lands managed by ONF, the Washington State Department of Natural Resources (DNR), and private landowners constitute a large percentage of the project area. The Bolton Peninsula divides Quilcene Bay from Dabob Bay, and the Toandos Peninsula borders the eastern edge of the project area. 3.1.1 Logistical Problems A round trip drive from the Jefferson County Public Health office in Port Townsend to the furthest extent of the project area is approximately 35 miles. The project budget allows for approximately 2-3 trips per month from the office to fieldwork sites and back. Efficient use of travel time and combining of activities will be needed to stay within budget. The project area is generally accessible by public and private roads. A number of private roads in the area are gated, locked, or posted and therefore inaccessible without permission of the landowner. Field staff will follow County guidelines on respect of privacy with regards to access to private property. Shoreline and stream monitoring stations are located on public right-of-ways such as roadsides, public land such as County, State Park or National Forest lands, or on private property on which the County has the landowner’s permission to access the site. Many of the streams to be monitored have year-round flows; however, some streams only have intermittent flows. Marine monitoring stations will be accessed by boat. Boat ramps are available at the Point Whitney Shellfish Lab and at Quilcene Boat Haven. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

3.1.2 History of Study Area

Pre-1850 In the early 1800’s Native American communities and seasonal settlements around Quilcene Bay and Dabob Bay included members of the Chem-a-kum, Dabop, Quilcene, Dosewallips, Skokomish, Tahuya, and Duhlelap tribes, and Euro-American settlements were sparse. 1850-1900 In 1855, as a result of the Point No Point Treaty, several tribes including the Skokomish and Chem-a-kum ceded their lands to the United States, reserving the right to continue fishing, hunting, and gathering in the ceded lands, in exchange for a small reservation at the south end of Hood Canal. Many small tribes were combined into the federally recognized Skokomish Indian Tribe. Settlement on the reservation proceeded rapidly; however, some Native Americans continued to live on or near their original lands. As late as the 1870s the Tarboo Valley, north of Dabob Bay, consisted of forested wetlands of Sitka spruce and Western red cedar, but by the 1890s the valley was being cleared for pasture and farmland. In 1889 the Union Pacific Railroad was making plans to link Port Townsend with the rest of the nation, and this brought settlers seeking their fortune in timber and land speculation. Due to financial difficulties Union Pacific abandoned the project in 1891, and many settlers left. In 1897 President Grover Cleveland designated the Olympic Peninsula's central high forests, where headwaters of the Dosewallips River originate, as the Olympic Forest Reserve. During this early era of logging, hand tools, donkeys, and oxen were routinely used to cut and move lumber in the Quilcene area. Olympia oysters, the only oyster species native to the area, were abundant along the shoreline. 1900-1950 In 1902 the Tubal Cain Mining Company claimed that Quilcene would become the center for smelting gold, iron, copper, and manganese expected to be found in the Olympics. Mining exploration continued until the 1920s but little ore was ever found. Quilcene Oyster Company, Quilcene Packing Company, and Quilcene Fish Hatchery began operations in 1911. In 1938 President Franklin Roosevelt signed the act establishing Olympic National Park in the area previously known as Olympic Forest Reserve. Outside of the Park, logging ramped up across the Olympic Peninsula providing timber to area mills, and advances in equipment and machinery led to increased harvest rates. Dairy farms were operating in much of the Tarboo valley by the early 1900s, but by the 1940s many were no longer in business. Native Olympia oyster populations decreased drastically in the early 1900’s due to overharvest and poor water quality conditions attributed to pulp mill discharges. In 1902 the Pacific oyster was first introduced from Japan, and because it was able to withstand poor water quality conditions and grew larger than native Olympia oysters, it soon became the mainstay of the State’s shellfish industry. The Rock Point Oyster Company purchased 200 acres of tidelands in Tarboo Bay in 1943. 1950-Present The U.S. Congress designated the Buckhorn Wilderness, where headwaters of the Big Quilcene River originate, in 1984. During that same year the Washington State Department of Natural Resources established the Dabob Bay Natural Area, which was enlarged in 2009, and currently includes 1,193 acres of mature coastal forests, coastal streams, feeder bluffs, forage fish spawning areas, eelgrass beds, native Olympia oyster beds, nearshore tidelands and mudflats utilized by juvenile salmon and shorebirds, and open marine waters utilized by salmon, marine birds, harbor seals and orcas. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

In the Tarboo Valley north of Dabob Bay, many large properties were subdivided and sold for rural residential uses by the 1990’s. In 2004, Northwest Watershed Institute (NWI) purchased the old Yarr farm property as the core of what would become the Tarboo Wildlife Preserve. In 2007 NWI began a habitat restoration project and re-meandered over three linear miles of Tarboo Creek and its tributaries, installed wood for habitat structure, and added spawning gravels to restore salmon spawning habitat and rearing areas for juvenile salmon. Tarboo Creek and several of its tributaries on the property provide important habitat for Coho and fall chum salmon, and cutthroat trout. The Preserve’s streams also support brook lamprey, crawfish, and freshwater mussels. Bear, cougar, deer, waterfowl and other mammals and birds also inhabit the property. Timber and logging practices over 150 years have removed most old growth forests surrounding Quilcene Bay and Dabob Bay. Current logging activity takes place primarily on private and state lands in low elevation forests. The shellfish industry remains prominent in the area. At the north end of Quilcene Bay, Coast Seafoods Company operates the world’s largest shellfish hatchery, capable of producing over 45 billion eyed oyster larvae per year that are provided to shellfish growers worldwide. Rock Point Oyster Company operates at the north end of Dabob Bay, producing mostly ground culture from natural set Pacific oysters and manilla clams. Taylor Shellfish Hatchery, built in 1990 near the head of Dabob Bay on the western shore, grows clam and oyster larvae in large tanks of seawater located on the uplands, and is one of the largest such hatcheries in the world. The Washington State Department of Fish and Wildlife (WDFW) operates the Point Whitney Shellfish Laboratory on the west shore of Dabob Bay, where various species of shellfish are reared, packed, and shipped. Quilcene Fish Hatchery, operated by U.S. Fish and Wildlife Service, has been in operation at the north end of Quilcene Bay since 1911. Salmon reared at the hatchery are released into Big Quilcene River, and also are provided to the Skokomish Indian Tribe for net pens in Quilcene Bay. Additionally, salmon eggs from Quilcene Fish Hatchery are provided to the WDFW George Adams Hatchery in Shelton, to be later transferred to Point-No-Point Treaty Council pens in Port Gamble Bay. Opportunities for recreational activities such as RV or tent camping, swimming, boating, fishing, and shellfish gathering are available at Dosewallips State Park, Seal Rock Campground, Cove RV Park, and Camp Parsons. Dosewallips State Park includes RV dump facilities, and Quilcene Boat Haven provides pump-out facilities for boaters to empty wastewater tanks. Fishing is regulated by WDFW, and several Native American tribes, including the Skokomish Tribal Nation, have fishing rights in the area. Communities in the area with town centers and businesses include Brinnon (see Figures 2 and 3) and Quilcene. A handful of other areas were developed somewhat densely as subdivisions in the later 1900s. Recent development has usually consisted of single-family homes in naturally distributed, individual projects. 3.1.3 Contaminants of Concern Quilcene and Dabob Bays are listed with a Category 5 impairment for bacteria on the EPA-approved 2012 303(d) list. Additionally, Big Quilcene River is listed as Category 2 for bacteria. For the 2014 Candidate 303(d) list, it has been proposed that both listings remain unchanged. Previous shoreline and stream monitoring by JCPH found elevated levels of fecal coliform and E. coli at numerous locations around the bays. Both bays have been identified as high priority work areas on the Hood Canal Regional PIC Priority Work Plan (Hood Canal Coordinating Council 2014). Considerable local concern has been expressed. The area has commercial and recreational shellfish beds that have experienced emergency closures due to contamination. Quilcene Bay and Dabob Bay experienced a Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 heavy marine algae bloom in September 2014 resulting in the highest ever levels of paralytic shellfish poison biotoxins measured in Washington oysters. Questions remain about the potential for non-point fecal sources being associated with nutrient pollution that could be aggravating harmful algae blooms. Shellfish and water recreation are important elements of the local economy and there is a strong desire to maintain open shellfish growing areas and safe water for recreation. 3.1.4 Results of Previous Studies Shoreline monitoring conducted from 2007-2011 by JCPH in the Hood Canal and Hood Canal Watershed Clean Water Projects found 21 “hot spots” for E. coli in Quilcene Bay and Dabob Bay (see Figure 4). Stream monitoring conducted during water year 2012/2013 found that streams that flow into Quilcene Bay and Dabob Bay were more impacted by fecal coliform than adjacent monitoring areas to the north or south in Hood Canal. Summary results from previous studies are provided in Tables 2-5. Both parts of the standard need to pass for the water quality standard for fecal coliform to be met.

Freshwater Quality Standard (Salinity<10 psu) Part 1 of the standard: the geometric mean value (GMV) should not exceed 50 FC/100 mL; Part 2 of the standard: not more than 10% of the samples should exceed 100 FC/100 mL).

Marine Water Quality Standard (Salinity >=10 psu) Part 1 of the standard: the geometric mean value (GMV) should not exceed 14 FC/100 mL; Part 2 of the standard: not more than 10% of the samples should exceed 43 FC/100 mL).

3.1.5 Regulatory Criteria or Standards Water quality standards are described in Chapter 173-201A of the Washington Administrative Code. Surface waters are protected based on designated uses and recreation. Freshwater streams in the project area are all designated for the highest level of protection, Extraordinary Primary Contact Recreation. All tributaries to Hood Canal, an extraordinary quality marine water body, which are identified in Table 602 of this Washington Administrative Code, must also meet the Extraordinary Primary Contact Recreation standards for fecal coliform bacteria. As stated previously, this is a two-part standard with a requirement that the geometric mean value not exceed 50 colony forming units (cfu) per 100 milliliters of water. The second requirement is that no more than ten percent of samples exceed 100 cfu/100 mL. Stream monitoring results will be compared against these State criteria (see Table 6).

4.0 Project Description 4.1 Project Goals The mission of Jefferson County Public Health is to protect the health of Jefferson County residents and visitors by promoting safe, healthy communities and environments. Clean water is essential to the health and well‐being of humans and wildlife. The overall goal of this project is to restore and protect surface waters for beneficial uses designated for the Hood Canal, which include extraordinary aquatic life habitat; shellfish harvest; primary recreational use. Non-point sources of pollution will be identified in Quilcene Bay and Dabob Bay, both high-priority areas of Hood Canal. Fecal bacteria and nutrient inputs will be analyzed and shoreline septic systems will be assessed through sanitary surveys, gaps in the existing permit records will be filled, and more will be known about high risk OSS in the project area. Correction activities will be performed to repair all high-risk failing systems. This will protect human Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 health from risks of waterborne pathogens and keep shellfish beds open and waters safe for recreation. Additionally, the relationship of freshwater quality and marine water quality will be better understood. Possible correlations of bacterial pollution and nutrient pollution will be assessed. It is hoped that this data could help future studies of nutrient ratios in relation to harmful algae blooms. The Quilcene-Dabob PIC project will address the Puget Sound Partnership’s Action Area Strategy through pollution prevention measures detailed in the Hood Canal Regional PIC Program. The project will: 1. Identify current pollution hotspots and areas of concern. 2. Evaluate nutrient inputs and their correlation with fecal bacteria and algal blooms, if any. 3. Implement Jefferson County Clean Water District mandates to monitor and control non -point pollution. 4. Implement portions of the Hood Canal Regional PIC Priority Work Plan. 5. Implement portions of the Jefferson County On-Site Sewage System Management Plan. 6. Implement portions of the 2011 WRIA 17 Watershed Management Plan and Detailed Implementation Plan. 4.2 Project Objectives Project objectives that pertain to Task 2 of the grant agreement, “Water Quality Monitoring and Analysis”, are as follows: 1. Submit a draft QAPP to Ecology for review and approval. 2. Complete 2 years of wet season and dry season monitoring of freshwater inputs (number of stations varies depending on flow) for fecal coliform and nutrients on 50 miles of shoreline. 3. Complete 2 years of wet season and dry season monitoring bimonthly for fecal coliform and nutrients at 16 marine stations. 4. Complete 1 year of stream monitoring at 17 freshwater stations for fecal coliform (see Figure 9). 5. Submit water quality data annually to the Department of Ecology via the Environmental Information Management (EIM) database. 4.3 Information Needed and Sources JCPH staff will use streamflow data from Ecology’s River and Stream Flow Monitoring in order to calculate and compare loadings. (https://fortress.wa.gov/ecy/wrx/wrx/flows/regions/state.asp). 4.4 Target Population Shoreline sources of possible fecal pollution along 50 miles of coastline surrounding Quilcene Bay and Dabob Bay will be our target population, including residential, recreational, and commercial properties. High priority properties include those associated with bacteria “hot spots” as determined by the water quality sampling in task 2, and those within 500 feet of the shoreline that have no septic system permit, or were permitted and are 25 years or older. 4.5 Study Boundaries The study area is fully within Jefferson County and includes the watersheds of major tributaries to the Quilcene Bay and Dabob Bay outlined in Section 3.1. Monitoring locations are shown in Figure 9 and listed in Tables 14, 15, and 16. The tasks required are the same items listed in Section 4.2, Project Objectives. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

4.6 Tasks Required This project will involve four related tasks. This QAPP guides work conducted for Task 2. All of the project tasks from the grant agreement are listed below. Task 1: Project Administration/Management - Project oversight and tracking; preparation of project sub- contracts; preparation and submittal of progress reports, final report, and payment vouchers; preparation and submittal of Quality Assurance Project Plan. Administer the project in accordance with grant agreement and maintain project records. Task 2: Water Quality Monitoring and Analysis - JCPH will submit Quality Assurance Project Plan (QAPP) to ECOLOGY for review and approval prior to conducting any water quality sampling. The QAPP will outline the quality assurance methods and protocols JCPH will follow to assure the highest quality data collection and analysis work will occur throughout the project period. JCPH will conduct a minimum of four marine shoreline surveys in the project area, two during the wet season (October – April), and two during the dry season (May – September). During the shoreline survey, JCPH will sample freshwater inputs and 16 marine stations for fecal coliform bacteria and nutrients. Additionally, JCPH will conduct one year of stream monitoring at 17 stations for fecal coliform bacteria and nutrients. JCPH will identify areas of concern based on sampling results and take additional samples to locate possible onsite septic system (OSS) failure, agricultural contamination, or other sources. All sampling site location data will be collected via global positioning system (GPS) and mapped in a geographic information system (GIS). A trend analysis for fecal coliform utilizing old and new data will be performed for the Quilcene and Dabob Bay sites for which at least three years of data is available, to begin to characterize whether water quality is improving or worsening. All areas of concern will be investigated with additional sampling and dye testing as needed to help pinpoint sources (see Task 3). JCPH will repair, replace, and/or purchase additional monitoring equipment, as necessary, due to normal equipment will be eligible for purchase through this project: boat and associated equipment, field tablets, and field gear. The total cost of all equipment purchased under this project will not exceed $29,000. Changes in equipment type or any additional equipment purchase must have prior approval from the ECOLOGY Project Manager to be eligible for reimbursement. JCPH must submit all data to ECOLOGY using the Environmental Information Management System (EIM) annually. Task 3: Pollution Identification and Correction - JCPH will research all on-site septic system (OSS) records in the project area and prioritize OSS survey locations within the Quilcene and Dabob Bays project area based on risk factors. High priority properties include those associated with bacteria “hot spots” as determined by the water quality sampling in task 2 and those within 500 feet of the shoreline that have no septic system permit, or were permitted and are 25 years or older. The purpose of the sanitary surveys will be to identify and correct failing OSS contributing to surface water pollution. JCPH will complete a minimum of 200 sanitary surveys within the project area. JCPH will conduct sanitary surveys according to the 2014 Hood Canal Regional Pollution Identification and Correction Guidance. JCPH will utilize corrective actions on failing OSS systems through a combination of public education and enforcement (where necessary) of local solid waste and onsite sewage regulations. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Task 4: Public Outreach and Education - JCPH will communicate project goals and accomplishments through direct one-on-one interaction during sanitary surveys, and two public meetings. One public meeting will occur at the beginning of the project and one at the conclusion. Individualized OSS operations and maintenance, copies of OSS “as-built” drawings, if available, as well as Operations and Monitoring facts will be provided to sanitary survey respondents. JCPH will provide OSS financial assistance information in the form of Craft3 septic loans, USDA Rural Development grants, and any other locally available resources to survey respondents and to owners identified with failing systems. 4.7 Practical Constraints It is expected that we will encounter intermittent flows at some sample locations, making it difficult to collect enough data to determine if the location has a water quality problem. During the wet season, flooding may occur which could prevent sampling in the precise sampling location. In these instances, we will sample as close as possible to the sampling site as is safe, and note where the samples were collected. It is unlikely we will have a staff shortage as we have multiple staff in the office trained to take water quality samples. 4.8 Systematic Planning Process This project was planned utilizing guidance documents from Ecology and the Environmental Protection Agency. The primary guidance used was the Ecology Guidelines for Preparing Quality Assurance Project Plans for Environmental Studies (Ecology, 2004). The Ecology QAPP template for external, non-TMDL projects was used to draft this document. Also consulted was the Quality Assurance Project Plan Review Router and Checklist for Water Quality Grant and Loan Projects from Ecology.

5.0 Organization and Schedule 5.1 Key Individuals and Their Responsibilities Environmental Health Director, Jared Keefer. Jared will work with Mike Dawson to make decisions on how to conduct the project, respond to citizen concerns, and oversee the project. Water Quality Manager, Mike Dawson. Mike will coordinate team schedules and track required performance, budget issues, lab results, quality control and reports. He will allocate resources and address problems that arise. He reports to the Environmental Health Director. Project Team Leader, Alisa Hasbrouck. Alisa will track progress of field work, conduct field work and provide information to Mike for quarterly reports, and follow up on problem areas. Most of her time will be allocated to this project. She reports to the Water Quality Manager. Other water quality staff will be trained as needed to perform field work. SPECTRA Laboratories – Kitsap, LLC (formerly Twiss Analytical Laboratory), Nancy Parrott. Nancy will oversee laboratory services and reporting for all water quality sampling. 5.2 Special Training and Certifications All project staff are currently trained and familiar with the methods used in this project. Any new staff that are assigned to the project or hired during the project will be trained and supervised while learning these procedures. No special certification is required for the analyses proposed in this project. Project management will be accomplished similar to other current projects managed by JCPH. Field sampling SOPs are discussed in Section 8.1. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

5.3 Organization Chart The organization chart is provided in Figure 5. 5.4 Project Schedule The project schedule is provided in Table 10. 5.5 Limitations on Schedule In addition to limitations noted in 4.7 above, coordination between multiple projects will be required since most staff work in several programs. 5.6 Budget and Funding Ecology’s share is 75% of the total eligible project costs. Matching funds will be provided by the County in the form of cash match from Clean Water District funds. A detailed budget is provided in Table 8.

6.0 Quality Objectives Goals for data quality for fieldwork measurements and sampling are derived from the overall project goals and include: 1. Sampling position data will be accurate enough to allow resampling from the same location by someone unfamiliar with the area. This will be done via GPS latitude and longitude data. 2. Sample and measurement results will be comparable to other local data sets. 3. Fecal coliform results will be accurate enough to determine whether a stream meets or fails to meet state water quality standards and aid in the prioritization of areas where sanitary surveys need to take place.

General quality objectives for fieldwork and laboratory analysis are as follows: 1. All field staff will be trained in the methods outlined in this QAPP. 2. The Water Quality Manager shall supervise field staff to assure that standard methods are being followed. 3. Analysis of field replicate samples collected by JCPH and JCCD will meet measurement quality objectives. 4. The contracted laboratory will perform internal quality control pursuant to their Ecology Accreditation and approved QA manual. 5. Data will be reported by the lab to JCPH as contracted. 6. Data will be validated and verified by JCPH after entry to assess usability. 7. Data will be uploaded to EIM and checked for quality by JCPH before being finalized. 6.1 Decision Quality Objectives (DQOs) This project does not determine regulatory compliance with a standard; therefore there are no decision quality objectives. The main goal of the project is to remove impaired streams from the EPA 303(d) category 5 list through pollution identification and correction. 6.2 Measurement Quality Objectives (MQOs) Measurement quality objectives include targets for precision, bias and sensitivity. Goals for data collection are driven by the project objectives above (see Section 4.2). Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

6.2.1 Targets for Precision, Bias, and Sensitivity Precision Precision is defined as the measure of agreement among repeated measurements of the same property under identical or substantially similar conditions. Precision for samples collected during this project will be determined by:  Collecting field replicate samples for marine and stream locations at a rate of two per sampling event at randomly selected sites.  Collecting field replicates for shoreline locations at a rate of 1 replicate per 10 samples collected.  Calculating the relative percent difference (RPD) and percent relative standard deviations (% RSD) of the replicate pairs.  Maintaining documentation of any deviations from standard protocols.  Verifying that the lab has followed all QA/QC protocols as required. Collecting replicates that will provide a 10% replicate rate over the course of one water year (one sample set) for both watersheds. Sample results that are “non-detects” or “too numerous to count” shall not be used to measure precision. Fecal coliform field replicate pairs will be analyzed in a spreadsheet to determine RPD and % RSD. Equations are shown below: RPD = [(absolute difference between duplicate samples)/(mean of the samples)] * 100 % RSD = [(standard deviation of replicate samples)/(mean of samples]) *100 Bacteria samples tend to have a high % RSD compared to other water quality analyses. Also, higher variability in low bacterial results limits the effectiveness of RPD and % RSD for evaluating precision (Mathieu, 2006). For the purposes of this project it will be sufficient to calculate only the % RSD for fecal coliform samples exceeding a mean of 20 colonies/100 mL per pair. For sample sets with fewer than 10 replicates, or results less than or equal to 20 colonies/100 mL, the Project Manager will review the results to determine the usability of the data (Figure 6, MQO Analysis Process for Bacteria). The MQO for precision of 10 or more replicates greater than 20 colonies/100 mL shall be that no more than half of field replicate pairs exceed 20% RSD and no more than 10% of pairs exceed 50% RSD. Because each batch of nutrient samples will include less than 10 replicate pairs, the Project Manager will review results and determine the usability of the data (Figure 7, MQO Analysis Process for all Parameters, Excluding Bacteria). Lab duplicates for fecal coliform bacteria from 250mL field samples split in the lab will be collected from the stream sites at a rate of one each season (4 per water year). Analytical duplicates for fecal coliform bacteria and nutrients will be done by the lab once per year. Lab measurement quality objectives are provided in Table 9). Bias Bias is the consistent deviation of measured values from the true value, caused by systematic errors in a procedure. Bias within the project will be reduced to the extent practicable by the following:  Strict adherence to the sampling procedures of the project work plan.  Complete data collection and organization.  Regular maintenance and calibration of field equipment.  Periodic reviews and evaluations of field sampling procedures.  Analyzing data in an appropriate manner based upon essential considerations, such as temporal variations. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Bias will be evaluated in the lab by running method blanks every 10 samples for fecal coliform (see Table 4). Sensitivity The method detection limit (MDL) for fecal coliform is 1 colony forming unit (see Error! Reference source not found.Table 3). 6.2.2 Targets for Comparability, Representativeness, & Completeness Comparability To ensure that results from this project will be comparable to results obtained from other Jefferson County Clean Water Projects, the same standard operating procedures and laboratory analysis methods will be used throughout. Standard operating procedures for Jefferson County have been developed for Clean Water Projects and are periodically updated as needed. These procedures are based on similar work done by other local health jurisdictions in Puget Sound, as well as Ecology ambient monitoring. Therefore, results from this study should be comparable to other studies in the region. Representativeness The design of the study and sampling plan is aimed at collecting data that is representative of the target streams within the project area. Project design was based on past and current experience with similar projects in Jefferson County. Spatial and temporal distribution of sampling will be discussed in Section 7, Sampling Process Design. The design also aims to collect a sufficient amount of data to complete project tasks. Completeness Factors such as weather conditions, site access, equipment malfunctions, data usability, etc. may prevent the full realization of every sample collection event scheduled. Completeness will be assessed by examining:  The amount of samples compared to the planned amount  The number of samples received in good condition at the lab  The lab’s ability to produce usable results for each sample  Sample results accepted by the Water Quality Manager A goal of 80% valid results in monitoring will be sufficient to achieve project goals.

7.0 Sampling Process Design 7.1 Study Design The study design is based on past successful projects JCPH has conducted such as the Hood Canal Clean Streams Project (JCPH, 2015), Hood Canal Clean Water Project (JCPH, 2013), Hood Canal Watershed (JCPH & JCCC, 2014), and the Chimacum Creek Clean Water Project (JCPH, JCCD & NOSC, 2011). Design of the study and sampling plan is aimed at identifying areas of concern for fecal coliform contamination and non-point sources of fecal bacteria. Project design was based on past and current experience with similar projects in Jefferson County. The design also aims to collect a sufficient amount of data to complete project tasks. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

7.1.1 Sampling Locations and Frequency

Geographic locations Sampling locations will be determined by JCPH staff using a Geographic Positioning System (gps) receiver. Apple iPads running ESRI Collector software will be used to collect position locations in Geographic Information System (GIS) format. The typical accuracy is plus or minus ten feet. Locations will be mapped in GIS and verified compared with existing datasets such as recent aerial photos, hydrology and surface topography. Coordinates may be recorded by hand or stored as waypoints in the receiver to be downloaded at the office. The basic process for collecting location data with gps in the field will be: 1. Obtain a fix on as many satellites as possible 2. Note position accuracy (e.g. ± 10’) 3. Record coordinates or take waypoints (World Geodetic System 1984, Decimal Degrees) 4. If a good fix is not obtainable at the site due to dense tree canopy or deep valleys, move to clearer site and note the distance and direction from the waypoint to the true (sampling) site 5. In the office, verify locations in a GIS map; correct if needed 6. In GIS, calculate the NHD reach code and percentage, or use the NHD Tool in the EIM Map Search webpage at https://fortress.wa.gov/ecy/eimreporting/Map/Map.aspx?MapType=EIM Geographic data parameters are provided in Table 10.

Site Identification (Site ID) Existing monitoring sites are listed in Tables 12, 13, and 14. New monitoring sites will be given a unique site ID in a consistent format. Site IDs will be used for labeling samples, recording sample collection in the field notebook, and reporting lab results in EIM.

Frequency JCPH will conduct the following monitoring: Monitoring of Freshwater Inputs along 50 miles of Shoreline for Fecal Coliform and Nutrients  Wet Season: Quarterly monitoring Oct 2016 - March 2017, and Oct 2017 - March 2018  Dry Season: Quarterly monitoring April - Sept 2016, and April - Sept 2017

Monitoring of 16 Marine Stations for Fecal Coliform and Nutrients  Wet Season: Bimonthly monitoring Oct 2016 - March 2017, and Oct 2017 - March 2018  Dry Season: Bimonthly monitoring July - Sept 2016, April - Sept 2017, and Jan - March 2018

Monitoring of 17 Stream Stations for Fecal Coliform  Wet Season: Monthly monitoring Oct 2016 - March 2017  Dry Season: Monthly monitoring April - Sept 2016 DON/0.2 and JAK/0.0 are within tidal influence, and will only be monitored when tide conditions allow. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Confirmation Sampling Shoreline discharge samples with results greater than 200 FC/100ml are re-sampled 1-2 times to confirm pollution “hotspots”. Re-sampling should occur as soon as possible and must be collected within the same sampling season, e.g. if the samples were collected as part of a wet season event, then confirmation samples must also be collected during the wet season. This is important because a discharge can be a “hotspot” during the dry season, or during the wet season, or during both seasons. As the project progresses the threshold for resampling may be reduced if time and budget allow. The geometric mean value (GMV) of the 2-3 results is calculated. The drainage is confirmed as a bacterial “hotspot” that needs investigation when the GMV is 500 FC/100ml or greater. Discharges that have two high bacterial samples are potential threats to public health. Fecal source investigation should begin as soon as possible, instead of waiting for a third sample. The process for confirming high fecal coliform hits is outlined in Figure 8.

Field Replicates and Other Sampling-Related Activities To determine variability of bacterial concentration and field measurements, replicate data will be collected during fieldwork. For stream sampling, Fecal Coliform replicates will be collected from sites with expected higher concentrations of bacteria when possible. Replicates will be performed at each stream site at least once to achieve a 10% replicate rate. For shoreline sampling, replicates will be collected according to this schedule:  1 replicate for every 10 samples, or  1 replicate per day of sampling when less than 10 are collected. Labeling will be as above. The corresponding Site ID of the replicate will be recorded in the data book, but not on the bottle, to reduce possible laboratory bias. Results will be associated with the correct location after laboratory reporting based on field notebook notes. A flowchart describing the process of fecal pollution hotspot confirmation is provided in Figure 8. Typically, there will be several confirmed “hotspots” within a project area. These will be ranked according to the GMV and used to initiate investigation of the “hotspots” with the greater GMVs first. Potential fecal pollution sources will be evaluated and identified through property inspections and segment sampling. The process for hot spot investigation is outlined in Error! Reference source not found.11.

7.1.2 Parameters to be Determined Stream, shoreline, and marine samples shall be collected by JCPH staff at established monitoring stations, and analyzed for fecal coliform bacteria. Since fecal coliform is more commonly used by State agencies, it will be used to allow data to compare more directly with past and future data sets. Additionally, samples will be analyzed for the following nutrients: ammonia, nitrate+nitrite nitrogen, total kjeldahl nitrogen, and total phosphorous-P (see Table 15, Lab Measurement Methods). 7.1.3 Field Measurements No field measurements will be obtained beyond observations of updated site conditions during each survey. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

7.2 Maps or Diagrams Shoreline and Marine Monitoring Stations in Quilcene Bay and Dabob Bay are mapped in Figure 9, and a listing of sample sites is provided in Tables 12, 13, and 14.. 7.3 Assumptions Underlying Design It has been assumed that stream sampling in the project area will be fundamentally similar to sampling conducted on past projects in Jefferson County. It is assumed that portions of the project area will have less water to sample than other sample sites because the area has less surface water. However, even if a greater amount of sampling sites than planned are located during fieldwork, adaptive management will be used to adjust staffing and resources to accomplish project goals. 7.4 Relation to Objectives and Site Characteristics Bacteria concentrations and distribution in surface water is affected by seasonal changes. Warmer temperatures allow more bacterial reproduction in the environment; however summer weather dries up streams and limits dispersion via surface water. Autumn rains tend to mobilize bacteria that have accumulated in soils and sediments, but after a number of heavy rains and cooler temperatures, bacterial levels often drop. Sampling in both the wet and dry seasons will support the objectives of the project and ensure that most conditions are represented in the dataset. By conducting water quality monitoring, we can further define and pinpoint the hotspots to allow JCPH staff to focus corrective actions to the areas with the most significant contamination problems in the most cost-effective and efficient way. 7.5 Characteristics of Existing Data A majority of the existing data that is available for the project area is from previous studies where samples were analyzed for fecal coliform. Marine monitoring data for fecal coliform is available from Washington State Department of Public Health (DOH), and stream monitoring data for fecal coliform is available from JCCD. The choice of fecal coliform for this study will allow for comparison with the majority of the existing data sets. Existing shoreline monitoring data is available for E. coli, and existing BEACH program monitoring data is available for enterococcus. Marine monitoring data is available for fecal coliform MPN conducted 2011- 2015 by DOH. Existing data is available for stream monitoring conducted by JCPH during 2009 and 2012, for nitrate+nitrite nitrogen. The quality of the data set as well as extensive institutional knowledge of the area provides some general idea of fecal coliform contamination hotspot areas. Both fecal coliform and nutrient levels in the project area will be better understood after the completion of this project.

8.0 Sampling Procedures 8.1 Field Sampling SOPs Standard Operating Procedures (SOPs) used by JCPH staff were developed based on SOPs used by DOH, Ecology (Ecology, 2013) and EPA. These include EAP030 Standard Operating Procedures for the Collection of Fecal Coliform Bacteria Samples in Surface Water, v2.1 2011 (Ecology, 2013). EAP030 is available at http://www.ecy.wa.gov/programs/eap/qa/docs/ECY_EAP_SOP_FecalColiformSampling_v2_1EAP030.pd f Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

8.2 Containers, Preservation, Holding Times For fecal coliform, 125mL sterile plastic containers supplied by the contracted laboratory will be used to collect samples. If a sample is to split at the lab, a 250 mL sterile plastic container will be used. Samples will be placed immediately on ice in a cooler and kept below 8 degrees C in the dark and delivered to the lab. Holding times will not exceed 24 hours. Adequate time is needed to access monitoring stations and deliver samples before the end of the business day. With advanced notification, the lab is then able to incubate the samples before the 24 hour period of the first sample collected has expired. For nutrient parameters (ammonia-nitrogen, nitrate+nitrite nitrogen, total kjeldahl nitrogen, and total phosphorous-P), 500 mL HDPE containers containing sulfuric acid preservative supplied by the contracted laboratory will be used to collect samples. Samples will be placed immediately on ice in a cooler, kept below 6 degrees C, and delivered to the lab. Holding times will not exceed 28 days. 8.3 Invasive Species Evaluation JCPH complies with Ecology standard operating procedures regarding invasive species. No known Areas of Extreme Concern will be entered as part of this project. No sediment sampling will be done in this project. Standard practices regarding the cleaning and maintenance of gear will be sufficient for project needs. In particular, the following practices are relevant to this project:  Felt-bottomed wading boots that can spread New Zealand mud snail will not be used unless following decontamination procedures.  Boats and boat trailers, when used, will be thoroughly flushed and washed after each use at the Jefferson County Shop in Port Hadlock where cleaning materials are available.  All gear will be stored in a way to promote drying between uses. 8.4 Equipment Decontamination All equipment shall be rinsed in freshwater as needed during fieldwork and after. A 1:10 bleach:water solution will be available if extreme contamination is suspected; for example, contact with raw sewage effluent. However, since this project utilizes grab samples collected by hand, no equipment other than a sampling pole is exposed to contamination. The following standard procedures will be used to prevent contamination of samples:  Sampling areas will be approached from downstream while avoiding disturbing sediments and altering the character of the water being sampled.  Sample bottles will be fresh, sterile containers with factory seal or wrap that is removed only at the time of sampling.  Sample bottles will be handled with disposable gloves. An adequate supply of gloves will be brought in the field to allow frequent changes.  Sample bottle caps will not be touched on the inside, and if set down will be placed upside down on a clean surface. 8.5 Sample ID Sample bottles will be labeled in the field with permanent marker with the Site ID or field replicate number. Field replicates will not be labeled with Site ID so that bias will not be introduced during lab analysis. Instead, the Site ID for field replicate will be recorded in the field notebook. After results have been reported to JCPH, field replicate results will be associated with locations by looking up the Site ID in the field notebook. In addition to field replicate samples, split samples will be collected which will be divided at the lab and each analyzed separately. Spilt samples will be labeled in the field with the Site ID, the same as regular Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 samples. The lab will enter a “Sample Sub ID” in column U of the EIM results spreadsheet to report results for split samples. For example, a split sample collected at Site ID BQU/0.64 would get Sample Sub IDs BQU/0.64-A and BQU/0.64-B in the results spreadsheet. 8.6 Chain-of-Custody Samples will be placed on ice in coolers for transport to the laboratory. Sampling will be done in the morning and early afternoon hours and finished in time to allow for transportation time. Delivery will be made either by JCPH staff or by lab courier on the same day as sampling occurs. Chain of custody forms will accompany each batch of samples and will be signed by the sampler when relinquishing them and by lab staff when receiving them. A custom chain of custody form has been created for use with the contracted lab and is included in the Appendices. Forms will be retained by JCPH for record keeping. 8.7 Field Log Requirements Field data will be recorded in the Rite-In-The-Rain field notebooks, including information on sampling, measurements, locations and conditions. The following information will be recorded:  Staff name(s)  Date and time  General weather conditions including previous rain events  Site ID  Site description if it is a new location  Note to indicate whether a sample was taken  Coordinates of new locations (WGS84 in decimal degrees) and an estimate of position accuracy (e.g. ± 10’)  Field measurement values, (estimated flow if possible).  Field replicate numbers (R1, R2, etc. and what Site ID it corresponds to)  Additional observations such as site conditions, special sampling conditions, signs of wildlife, photos taken, etc. Dates should be written in international standard format (ISO 8601) in year, month, day, hour, minute order (from the largest time unit to the smallest). This eliminates confusion and allows filenames to be ordered by date and time. For example, 3:30 pm October 1st, 2012 would be written 2012-10-01-1530. Photos may be taken in the field to clarify site locations, and document site conditions or unusual water appearance.

9.0 Measurement Methods 9.1 Field Procedures/Field Analysis Samples will be collected by JCPH staff using the following techniques: the sample collection point will be approached with care taken not to disturb the bottom sediments. Stream sample locations will be approached from downstream, and samples collected while facing upstream, at the deepest accessible point or from cascade flow over rocks or logs, to avoid collecting disturbed sediment. When possible, the sample will be taken below the top 1 inch of water, but not when this would disturb bottom sediments. Sources and sampling technique will be documented in the field notebook. Samples may be collected by hand or with a sample-pole. For fecal coliform analysis, 100 mL of water are collected in sterile 125 ml bottles. For nutrient analysis (ammonia-nitrogen, nitrate+nitrite nitrogen, total kjeldahl nitrogen, and total phosphorous), 500 mL of water are collected in HDPE bottles containing sulfuric acid Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 preservative. For samples collected in bottles containing preservative, care will be taken to prevent the loss of preservative by spilling or overfilling. Each bottle will be clearly labeled with the Site ID, which will serve as the sample ID. Samples shall be placed in a cooler with ice. Additionally, notes will be made to record any unusual odors, warm temperature, matting, unusual vegetative growth, foam, laundry lint, food waste, or other characteristics that can indicate intermittent sewage sources, animal waste or tracks near the sample point, unusual color in the sample, etc. Low or stagnant stream flows will also be noted. If it is difficult to collect a sample due to very low flows, a small hole may be dug to create a fall from which a sample can be collected, if it is possible to do so without collecting disturbed sediment. If it is not possible to collect the full 100 mL sample, at least 25 mL will be collected and diluted by the lab, with field notes recorded about the collection conditions and methods. Sample delivery will be prompt so that analysis can begin within applicable holding times. Samples will be taken Monday through Thursday to allow for analysis time. Sampling will not occur on Fridays. No field analyses are planned in this project. Field equipment and supplies will be assembled prior to fieldwork. Field equipment and supplies are listed below.  iPad  125 mL sterile plastic sample bottles, for fecal coliform analysis  500 mL HDPE sample bottles, for nutrient parameter analyses  Sampling pole  Rite-In-The-Rain field notebooks and/or data sheets, pencil, clipboard  Chain of custody form  Permanent marker for labeling samples  Cooler with ice  Wet weather gear, boots, gloves  Cell phone  First aid kit 9.2 Laboratory Procedures Laboratory procedures are derived from Standard Methods for the Examination of Water and Wastewater (Rice, Baird, Eaton, & Clesceri, 2012) for each analyte. Laboratory methods for the analytes to be sampled are described in Table 15. Dilutions Quantified bacterial analysis can result in values that are “too numerous to count” (TNTC). This data cannot be used to calculate geometric means without substituting a number for each TNTC result. Geometric means need to be calculated to determine compliance with state water quality standards. Usually a number such as one significant digit above the upper limit of detection is substituted when computing the geometric mean with TNTC values, however different practices are used by different agencies and no consensus exists. In an effort to lessen the frequency of TNTC results and make results calculations easier, dilutions may be requested of the laboratory by the field sampler. Dilutions values requested on the chain of custody form may be none, 1:2 or 1:10. A 1:10 dilution is standard lab practice for fecal coliform membrane filtration analysis of freshwater environmental samples. Another use of dilutions is when 100 mL of water is not available to sample, such as during very low flows. For example, during sample collection a pipe may be found with a slight drip where sampling more than 25 mL of water may take a long time to collect and be impractical for field staff. Note that dilutions effectively raise the method detection limits by whatever factor of dilution is used. However, Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 for the purposes of this study the lower range of detection is not important to meet project goals. The lowest regulatory level of fecal coliform is 50 cfu/100mL, for extraordinary primary contact. At the highest level of dilution (1:10), this still allows detection of fecal coliform down to 10 cfu/100mL. Dilutions also raise the upper limit of the reporting range. Treatment of non-detects and TNTC data will be discussed in the Section 14.3. All laboratory data will be entered into the JCPH database and listed as “Preliminary”. It will be verified by project staff and given the designation of “Accepted” or “Rejected”. If rejected, the Result_Rejected_Flag field in EIM will be marked with a “Y” (yes) and the reason for the rejection will be entered in the Result_Comment field. Data Quality (Usability) Assessment is discussed in Section 14.0 below. 9.2.1 Analytes Analytes for this project include fecal coliform, ammonia-nitrogen, nitrate + nitrite nitrogen, total kjeldahl nitrogen, and total phosphorous-P (see Table 15). 9.2.2 Matrix The sample matrix at shoreline input stations and stream stations is freshwater. The sample matrix at marine stations is brackish. 9.2.3 Number of Samples The approximate number of sample stations, sampling events, and total number of samples expected to be collected over the project period is provided in Table 16. 9.2.4 Expected Range of Results The expected minimum and maximum range as well as geometric mean and standard deviation values for fecal coliform results based on prior studies for Quilcene Bay and Dabob Bay conducted by JCPH and Jefferson County Conservation District, 2007-2015 is provided in Table 17. 9.2.5 Analytical Methods Analytical methods to be used for this project are provided in Table 3. 9.2.6 Sensitivity/Method Detection Limit (MDL) In laboratory analysis, sensitivity is the smallest change in concentration of an analyte that can be detected using a specific lab method. Method detection limit is the lowest concentration of an analyte that can be detected using a specific lab method (see Error! Reference source not found.15). 9.3 Sample Preparation Method No unusual sample preparation methods are anticipated outside of the Standard Methods. 9.4 Special Method Requirements No special method requirements are anticipated for this project. 9.5 Lab(s) Accredited for Methods Only Ecology-accredited laboratories will be used for this project. Currently, JCPH uses the laboratory services of Spectra Laboratories-Kitsap, LLC in Poulsbo, Washington. If Spectra is unavailable during sampling events, an alternate lab such as Edge Analytical’s microbiology lab in Bellingham will be used. Only laboratories that are accredited by Ecology for the analyses planned in this project will be used. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

10.0 Quality Control Field replicates will be collected every 10 samples, or every sampling event if less than 10 are collected (see Table 4). Statistical analysis of replicate pairs will be conducted frequently to allow for any adaptation to the sampling regime needed to accomplish project goals. Field replicates are believed to be an adequate measure of precision for this project based on past results and no field blanks are planned. Laboratory quality control will be performed for each batch of samples submitted. Samples of 250ml will be collected once a year and split for lab analytical duplicates. For nutrient parameters, matrix spikes will be used at a rate of one per 10 samples. For the microbiological methods being used, matrix spikes are not applicable. 10.1 Table of Lab and Field QC Required Lab and field QC samples, types, and frequency are provided in Table 4. 10.2 Corrective Action Corrective action may be required if MQOs in Figures 6 and 7 are not met. For field replicates this would require: 1. Flagging of data that do not meet MQOs 2. Review of field sampling to ensure that SOPs are being followed 3. Scheduling of additional sampling as needed to meet collection targets Results that did not meet MQOs will result in a Result Value Rejected Flag of “Y” and notes in the Result Additional Comment fields of the EIM spreadsheet. Laboratory MQOs are addressed by the Standard Methods in use at the lab. Any difficulties meeting analysis QC requirements will be discussed with the lab to determine the proper remedy.

11.0 Data Management Procedures 11.1 Data Recording/Reporting Requirements The contracted lab will report results to JCPH within one week after analysis. Final reporting will be done by JCPH at project conclusion, including graphs, charts, maps, quality control analysis and a narrative summarizing the results. A draft of the final report will be submitted to the Ecology grant manager for review by November 15, 2016. Reporting requirements are summarized in Table 19. 11.2 Lab Data Package Requirements Laboratory results will be reported to JCPH in a timely manner. The lab agreement stipulates the turnaround time for data reporting. Fecal coliform results will generally not be needed right away, so reporting within 4 days will be sufficient. At a minimum, a hard copy of the chain of custody with results entered by the lab will be submitted by the lab to JCPH with monthly billing invoices. 11.3 Electronic Transfer Requirements In all cases, lab data reporting includes delivery of an electronic version in EIM spreadsheet format (Microsoft Excel) within 1 month for the purposes of data entry. This will help to eliminate data entry errors when transferring lab results to JCPH databases or spreadsheets. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

11.4 Acceptance Criteria for Existing Data There are no special criteria necessary to assess the usability of existing data. 11.5 EIM/STORET Data Upload Procedures Lab results from sample analysis will be submitted to Ecology via the EIM database following each water year, either by crosswalk from database to database or by the spreadsheet method. JCPH has an organization account configured in EIM. A project named Quilcene and Dabob Bay Pollution Identification and Control will be created in EIM to hold the data. The JCPH Water Quality Manager will use the grant number G16-00039 as the Study ID to associate all the data with the project. JCPH will submit data to EIM annually by December 31 for the previous water year.

12.0 Audits and Reports 12.1 Audit Number, Frequency, Type and Schedule Not applicable. There is no need for audits for this study. 12.2 Responsible Personnel Responsible parties are provided in Table 19. 12.3 Frequency and Distribution of Reports JCPH will submit water quality monitoring data to EIM annually by December 31 for the previous water year. A final report for the project will be prepared by JCPH that summarizes the results including QC data. All records associated with the project will be retained according to the County records retention schedule and will be available for review. 12.4 Responsibility for Reports Reports will be the responsibility of JCPH project staff.

13.0 Data Verification 13.1 Field Data Verification, Requirements, and Responsibilities Field measurements will not be collected for this project. 13.2 Lab Data Verification The lab will perform internal data verification before releasing data to JCPH. The lab will report to JCPH in the event that holding times are exceeded for samples or that preservation temperatures exceeded lab method requirements. In these cases, the project manager will make a decision whether the samples should be analyzed. If the samples are analyzed, notes will be added to the result comment field. JCPH will review all lab data for accuracy and completeness before data entry. Geographic data will be verified by methods outlined above (see Section 7.1.1). All data will be validated during submission of data to EIM. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

13.3 Validation Requirements All laboratory data will be entered into the JCPH database and listed as “Preliminary”. It will be verified by project staff and given the designation of “Accepted” or “Rejected”. If rejected, the Result_Rejected_Flag field in EIM will be marked with a “Y” (yes) and the reason for the rejection will be entered in the Result_Comment field.

14.0 Data Quality (Usability) Assessment 14.1 Process for Determining Whether Project Objectives Have Been Met Usability determination will entail evaluation of field and laboratory results and relative standard deviation between field replicates. Adherence to established protocols should eliminate most sources of bias. Field replicates should indicate overall variability (environmental, sampling, and laboratory). The Water Quality Manager will verify that all measurement and data quality objectives have been met for each sampling event. If the objectives have not been met (such as % RSD for field replicates exceeding the MQO) then consideration will be taken to qualify the data, how to use it in analysis, or whether it should be rejected. Decisions for data quality and usability will be documented. 14.2 Data Analysis and Presentation Methods Data analysis will be conducted by JCPH. Accepted fecal coliform data will be used to calculate geometric means for comparison to State Water Quality standards. Geometric means will be calculated according to the following formula:

th Geometric Mean = n root of (X1)(X2)…(Xn)

Where X1, X2, etc. represents individual data points and n is the total number of data points used in the calculation. 14.3 Treatment of Non-Detects Substitutions may be made for non-detects and TNTC data in the calculation of geometric means for internal work. The method and rationale for substitutions used in this project will be described in the final report. Substituted values will not be submitted to EIM. Instead the EIM data coordinator’s recommendations will be followed. EIM will require data qualifier codes to be entered with certain results. For non-detects, the code “U” (Analyte was not detected at or above the reported estimate) is added. TNTC results receive code “E” (Reported result is an estimate because it exceeds the calibration range). The EIM coordinator’s recommendations are summarized in Table 20. 14.4 Sampling Design Evaluation The project manager will decide whether the data package meets the MQOs, criteria for completeness, representativeness, and comparability, and whether meaningful conclusions (with enough statistical power) can be drawn. If so, the sampling design will be considered effective. 14.5 Documentation of Assessment In the final report, the project staff will include a detailed summary of the data quality assessment along with the full analysis and findings of the surveys. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

15.0 References Coast Seafoods Company. (2016). Retrieved February 2016 from http://www.coastseafoods.com/. DNR. (2016). Retrieved from Dabob Bay Natural Preserve: http://www.dnr.wa.gov/dabob-bay-natural- area-preserve. East Jefferson Watershed Council. (2011). Watershed Management Plan and Detailed Implementation Plan for the Quilcene-Snow Water Resources Inventory Area (WRIA 17). Ecology. (2012). 303(d) List. Retrieved February 2016, from 2012 WQ Search Tool: https://fortress.wa.gov/ecy/wats/approvedsearch.aspx. Ecology. (2004). Guidelines for Preparing Quality Assurance Project Plans for Environmental Studies. Publication number 04-03-030. Lacey: http://www.ecy.wa.gov/biblio/0403030.html. Ecology. (2013). Quality Assurance at Ecology. Retrieved March 2013, from Environmental Assessment Program: http://www.ecy.wa.gov/programs/eap/quality.html. Ecology. (2006). Replicate Precision for 12 TMDL Studies and Recommendations for Precision Measurement Quality Objectives for Water Quality Parameters. Retrieved March 2013, from: www.ecy.wa.gov/biblio/0603044.html. Ecology. (2012). Standard Operating Procedures to Minimize the Spread of Invasive Species, Version 2.0. Ecology. (2011). Water Quality Standards for Surface Waters of the State of Washington, Chapter 173- 201A WAC. Ecology. (2011). Waters Requiring Supplemental Spawning and Incubation Protection for Salmonid Species. Publication number 06-10-038. Olympia: http://www.ecy.wa.gov/biblio/0610038.html. EPA. (2000). EPA Quality Manual for Environmental Programs. Publication number CIO 2105-P-01-0. Washington, DC: http://www.epa.gov/sites/production/files/2013-10/documents/2105p010.pdf. EPA. (1997). Glossary of Quality Assurance Terms and Related Acronyms. http://www.ecy.wa.gov/programs/eap/qa/docs/epa_quality_glossary.pdf. EPA. (2000). Guidance for the Data Quality Objectives Process. Publication number EPA QA/G-4. Washington, DC: http://nepis.epa.gov/Exe/ZyPDF.cgi/P100JE1J.PDF?Dockey=P100JE1J.PDF. EPA. (2006). Guidance on Systematic Planning Using the Data Quality Objectives Process EPA QA/G-4. http://www.epa.gov/sites/production/files/2015-06/documents/g4-final.pdf. EPA. (2012). Recreational Water Quality Criteria. Washington: water.epa.gov/scitech/swguidance/standards/criteria/health/recreation/index.cfm. HistoryLink.org. (2016). Retrieved February 2016, from Jefferson Count--Thumbnail History: http://www.historylink.org/index.cfm?DisplayPage=output.cfm&file_id=7472. Hood Canal Coordinating Council. (2014). Hood Canal Regional Pollution Identification and Correction Five-Year Priority Area Work Plan. Kammin. (2010). Definition developed or extensively edited by William Kammin, 2010. Northwest Watershed Institute. (2015). Retrieved February 2016, from Programs & Projects: http://nwwatershed.org/tarboo-wildlife-preserve.html. Olympic Peninsula Intertribal Cultural Advisory Committee. (2002). Native Peoples of the Olympic Peninsula: Who We Are. University of Oklahoma Press, Norman Publishing Division. Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Pacific Coast Shellfish Growers Association, Steele, David. (2013). Rock Point Oyster Co, Inc. Retrieved February 2016, from http://pcsga.org/wprs/wp-content/uploads/2013/04/Winter-2012-Grower- Profile.pdf. Peninsula Daily News. (February 17, 2013). Peninsula Daily News Back When - History Wends Along Mountain Trails. Port Townsend Leader. (2015). DNR buys lands around Taylor Shellfish Hatchery for long-term conservation, Retrieved February 2016 from http://www.ptleader.com/news/dnr-buys-lands-around- taylor-shellfish-hatchery-for-long-term/article_393b46e8-7d02-11e5-9d5b-13140a8843ff.html. Pritzker, Barry M. A Native American Encyclopedia: History, Culture, and Peoples. Oxford: Oxford University Press, 2000. ISBN 978-0-19-513877-1. Rice, E., Baird, R., Eaton, A., & Clesceri, L. (2012). Standard Methods for the Examination of Water & Wastewater, 22nd Edition. American Public Health Association (APHA), American Water Works Association (AWWA) & Water Environment Federation (WEF). US Census Bureau. (2010). Retrieved February 2016, from Community Facts Search Tool: http://factfinder.census.gov/faces/nav/jsf/pages/community_facts.xhtml. USFW. (2016). Retrieved February 2016, from Quilcene National Fish Hatchery: http://www.fws.gov/quilcenenfh/index.cfm. USFW. (2006). Quilcene National Fish Hatchery Report. Retrieved February 2016 from http://www.fws.gov/pacific/Fisheries/Hatcheryreview/Reports/olypen/QL--004QL2006color.pdf. USGS. (1998). Principles and Practices for Quality Assurance and Quality Control. Open-File Report 98- 636. http://ma.water.usgs.gov/fhwa/products/ofr98-636.pdf. 16.0 Figures

Figure 1, Study Area Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Figure 2, 2000 aerial photo of Brinnon

Figure 3, 2013 aerial photo of Brinnon

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Figure 4, E. coli & Sanitary Survey Results, 2007-2014

Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Figure 5, Project Organizational Chart

Figure 6, MQO Analysis Process for Bacteria (Mathieu, 2006)

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Figure 7, MQO Analysis Process for all Parameters, Excluding Bacteria (Mathieu, 2006)

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Sample drainage

Do results No exceed threshold? Yes

No Action Conduct confirmation sampling (1 -2 times) within Same season and calculate geometric mean value

Do results exceed No threshold? Yes

Any result No Yes Investigate drainage >500?

No Action

Figure 8, Hotspot Confirmation

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Figure 9, Shoreline and Marine Monitoring Stations, Quilcene-Dabob Bay Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 17.0 Tables Table 1, Measurement Quality Objectives Parameter Field Laboratory Precision Precision Bias Sensitivity Check Method Method detection limit Standards Blanks Fecal coliform (<20 cfu, see Fig 2) Positive and < MDL 1 cfu (membrane No more than 50% of negative controls filtration) replicates >20% RSD; No more than 10% of replicates >50% RSD Ammonia- NA Positive and < MDL 0.003 mg/L Nitrogen negative controls Nitrate+Nitrite NA Positive and < MDL 0.003 mg/L Nitrogen negative controls Total Kjeldahl NA Positive and < MDL 0.02 mg/L Nitrogen negative controls (freshwater matrix) Total Kjeldahl NA Positive and < MDL 0.03 mg/L* Nitrogen negative controls (marine water matrix) Note*: TKN digestions must be modified for marine waters which increases the MDL over fresh water values.

Table 2, Sample Containers, Preservation, and Holding Times

Container/Volume Analyte Matrix Method (mL) Preservative Holding Time Fecal Coliform Freshwater SM 9222D 125 mL sterile <8° C, dark <24 hours plastic

Ammonia- Freshwater SM 4500 500 ml Test ASAP or H2SO4, 28 days Nitrogen ≤6oC NH3-G HDPE

o Nitrate+Nitrite- Freshwater SM 4500 500 ml H2SO4, ≤6 C 28 days Nitrogen NO3-F HDPE

o Total Kjeldahl Freshwater EPA 351.2 500 ml H2SO4, ≤6 C 28 days Nitrogen HDPE

o Total Freshwater SM 4500 500 ml H2SO4, ≤6 C 28 days Phosphorous-P P-E HDPE

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 April 13, 2016

Table 3, Measurement Methods (Lab)

Method Range Detection Limit Possible Range at 1:2 Range at 1:10 MDL at 1:2 MDL at 1:10 Analyte Matrix Method (no dilution) (MDL) dilutions dilution dilution dilution dilution

Fecal Coliform Freshwater or SM 9222D <1 to >200 1 cfu/100mL None, 1:2, <2 to >400 <10 to >2,000 2 10 cfu/100mL Marine Water cfu/100mL 1:10 cfu/100mL cfu/100mL cfu/100m L Ammonia-Nitrogen Freshwater SM 4500, NH3-G 0.01 - 5 mg/L 0.003 mg/L No Limit 0.01-10 mg/L 0.01 – 50 mg/L 0.003 mg/L 0.003 mg/L Ammonia-Nitrogen Marine Water* SM 4500, NH3-G 0.01 - 5 mg/L 0.003 mg/L No Limit 0.02-10 mg/L 0.1 – 50 mg/L 0.006 0.03 mg/L** mg/L** Nitrate+Nitrite- Freshwater SM 4500, NO3-F 0.01 - 5 mg/L 0.003 mg/L No Limit 0.01-10 0.01 – 50 0.003 mg/L 0.003 mg/L Nitrogen mg/L mg/L Nitrate+Nitrite- Marine Water* SM 4500, NO3-F 0.01 - 5 mg/L 0.003 mg/L No Limit 0.02-10 0.1 – 50 mg/L 0.006 0.03 mg/L** Nitrogen mg/L mg/L** Total Kjeldahl Freshwater EPA 351.2 0.2 - 5 mg/L 0.02 mg/L No Limit 0.2-10 0.2 – 50 mg/L 0.02 mg/L 0.02 mg/L Nitrogen mg/L Total Kjeldahl Marine Water EPA 351.2 0.4 - 5 mg/L 0.03 mg/L*** No Limit 0.8-10 4.0 – 50 mg/L 0.06 0.3 mg/L** Nitrogen mg/L mg/L** Total Phosphorous-P Freshwater SM 4500 P-E 0.01 - 5 mg/L 0.005 mg/L No Limit 0.01-10 0.01 – 50 0.005 mg/L 0.005 mg/L mg/L mg/L Total Phosphorous-P Marine Water* SM 4500 P-E 0.01 - 5 mg/L 0.005 mg/L No Limit 0.02-10 0.1 – 50 mg/L 0.01 0.05 mg/L** mg/L mg/L**

1. *Standard Methods for ammonia, nitrate + nitrite, and total phosphorous have accommodations for salt water samples incorporated into the method. Dilutions to solve matrix problems are generally not needed and this allows the reporting limit (low range) to stay the same.

2. ** If matrix effects were encountered which required dilution, but the result was below the reporting limit, that limit would then be multiplied by the dilution factor.

3. ***TKN digestions must be modified for marine waters which increases the MDL over fresh water values.

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Table 4, QC Samples, Types and Frequency Field Laboratory Parameter Blanks Replicates Check Method Analytical Matrix Standards Blanks Duplicates Spikes Fecal Coliform none ≥10% of total Positive and every Once a NA samples; (every negative 10 year 10 samples or controls samples every sampling every lot of event if less than media 10) Nutrients (ammonia- none ≥10% of total Positive and every Once a One per nitrogen, samples; (every negative 10 year 10 nitrate+nitrite 10 samples or controls samples samples nitrogen, total every sampling kjeldahl nitrogen, event if less than total phosphorous-P) 10)

Table 5. Hood Canal Watershed Phase 1 2011-12, Water Quality Standards Pass/Fail 2011-12 April to May June to September Stream Station Part 1 Part 2 Part 1 Part 2 Marple Creek MAR/0.0 Pass Pass Pass Pass Spencer Creek SPE/0.0 Pass Pass Pass Fail Turner Creek TUR/0.0 Pass Pass Pass Pass 2012-13 June to September June to September Part 1 Part 2 Part 1 Part 2 Big Quilcene River BQU/0.64 Pass Pass Pass Pass Cemetery Drain CDR/0.6 Fail Fail Fail Fail Indian George Creek IND/0.2 Pass Pass Pass Fail Little Quilcene River LQU/0.8 Pass Pass Pass Pass 2013-14 October to May June to September Part 1 Part 2 Part 1 Part 2 Tarboo Creek TBO/0.9 Pass Fail Fail Fail

Table 6, Hood Canal Clean Streams Dec 2014 – Jan 2016, Water Quality Standards Pass/Fail Dec 2014 - Jan 2016 Stream Station Part 1 Part 2 Big Quilcene River BQU/0.64 Pass Pass Cemetery Drain CDR/0.6 Pass Fail Little Quilcene River LQU/0.1 Pass Fail Donovan DON/0.2 Pass Fail Tarboo Creek TAR/0.9 Pass Fail

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Table 7. Dept. of Ecology Regional Stormwater Monitoring Data, Jan-Nov 2015

Location ID Sample Date Parameter Result (Little Quilcene River) (cfu/100mL) 054-OUGA 1/21/2015 Fecal Coliform 1 054-OUGA 2/25/2015 Fecal Coliform 1 054-OUGA 2/25/2015 Fecal Coliform 1 054-OUGA 3/17/2015 Fecal Coliform 2 054-OUGA 4/14/2015 Fecal Coliform 2 054-OUGA 5/13/2015 Fecal Coliform 1

Table 8, BEACH Monitoring Data, 2004-2014 Weekly Enterococcus Average Location Beach ID # of weeks > 60 # of weeks > 104 # of weeks > 276 Camp Parsons WA241567 6 6 5 Herb Beck Marina WA700887 9 6 3 Point Whitney Tidelands WA278114 5 3 2

Table 9, Water Quality Standards for Fecal Coliform Washington Administrative Code - Freshwater Water Quality Criteria

Project Area Parameter Water Quality Criteria Date Waterbody Criteria Freshwater; Fecal coliform Part 1 Geomean ≤ 50 cfu/100 mL All year Extraordinary Fecal coliform Part 2 90% samples ≤ 100 cfu/100 mL All year Primary Contact Recreation Marine; Fecal coliform Part 1 Geomean ≤ 14 cfu/100mL All year Primary Contact Fecal coliform Part 2 90% samples ≤ 43 cfu/100 mL All year Recreation

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Table 10, Project Schedule Quarter 1 (Jan-Mar) Quarter 2 (Apr-Jun) Quarter 3 (Jul-Sep) Quarter 4 (Oct-Dec) Wet season Dry season Dry season Wet season 2016  Quarterly Progress Report  Quarterly Progress Report  Quarterly Progress Report  Quarterly Progress Report  Submit draft QAPP by 4/1/16  Quarterly monitoring of  Quarterly monitoring of  Quarterly monitoring of  Obtain equipment freshwater inputs freshwater inputs freshwater inputs  Research OSS records and conduct  Bimonthly monitoring of  Bimonthly monitoring of sanitary surveys marine stations marine stations  Public meeting  Research OSS records and  Monthly monitoring at conduct sanitary surveys stream stations  Annual submittal of water quality data to DOH via EIM  Research OSS records and conduct sanitary surveys 2017  Quarterly Progress Report  Quarterly Progress Report  Quarterly Progress Report  Quarterly Progress Report  Quarterly monitoring of freshwater  Quarterly monitoring of  Quarterly monitoring of  Quarterly monitoring of inputs freshwater inputs freshwater inputs freshwater inputs  Bimonthly monitoring of marine  Bimonthly monitoring of marine  Bimonthly monitoring of  Bimonthly monitoring of stations stations marine stations marine stations  Monthly monitoring at stream  Monthly monitoring at stream  Monthly monitoring at  Annual submittal of water stations stations stream stations quality data to DOH via EIM  Research OSS records and conduct  Research OSS records and conduct  Conduct sanitary surveys  Conduct sanitary surveys sanitary surveys sanitary surveys  Complete research of OSS  Send residents water quality data records from samples collected April 2016-

March 2017 2018  Quarterly Progress Report  Quarterly Progress Report  Quarterly Progress Report  Quarterly Progress Report  Closeout Report due 3/31/18  Bimonthly monitoring of marine  Conduct sanitary surveys  Project Outcome Summary  Quarterly monitoring of freshwater stations Report due 11/30/18 inputs  Conduct sanitary surveys  Annual submittal of water  Bimonthly monitoring of marine  Send residents water quality data quality data to DOH via EIM stations from samples collected April 2017-  Public meeting  Conduct sanitary surveys March 2018

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Jefferson County Public Health Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Table 11, Budget by Task Quilcene-Dabob PIC TOTAL ELIGIBLE ECOLOGY JCPH Match COST TASKS 1 – Project Administration/Management $72,450.00 $54,337.00 $18,113.00 2 – Water Quality Monitoring and Analysis $235,800.00 $176,850.00 $58,950.00 3 – Pollution Identification and Correction $149,925.00 $112,444.00 $37,481.00 4 – Public Outreach and Education $33,437.00 $25,078.00 $8,359.00 Total $491,612.00 $368,709.00 $122,903.00

Table 12, Geographic Data Parameters Collection method Handheld gps receiver Accuracy ± 3 meters or better Datum WGS84 Coordinate system Latitude, Longitude Coordinate format Decimal degrees (DD) Latitude range (for data verification) 48.146282 to 47.962343° Longitude range (for data verification) -122.666707 to -122.894096°

Table 13, Hotspot Investigation STEP 1 Confirm “hotspot” during dry or wet season by collecting a minimum of 2-3 samples for GMV calculation. STEP 2 Create map of the area within 200 feet of the “hotspot”. STEP 3 Conduct reconnaissance to assess # of homes, proximity to drainage, presence of livestock, possible access points for segment sampling, etc. STEP 4 If # homes ≤10, review onsite sewage system (OSS) records for all homes and inspect them. STEP 5 If # homes >10, conduct segment sampling, starting at the discharge and collecting samples uphill toward the source. Collect minimum 3 sampling events and calculate geometric mean for each segment. Note that segment sampling must occur during the same season that “hotspot” was confirmed.

 If drainage is high throughout, conduct OSS record search and inspect all homes within 200 feet.  If drainage is not high throughout, conduct OSS record search and inspect all homes within 200 feet of the “hotspot” segment(s).

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Table 14, Shoreline Monitoring Stations, Quilcene-Dabob Bay Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ JEF030A Quilcene Bay 47.80111 -122.86700 JEF030B Quilcene Bay 47.80103 -122.86730 JEF030C Quilcene Bay 47.80089 -122.86756 JEF032A Dabob Bay 47.76201 -122.85383 JEF032B Dabob Bay 47.76200 -122.85194 JEF032C Dabob Bay 47.76180 -122.85098 JEF033A Jackson Cove 47.74477 -122.86659 JEF033B Jackson Cove 47.74513 -122.86989 JEF033C Jackson Cove 47.74451 -122.87177 MAR/0.0 Marple Creek 17110018000466 8.7 47.73960 -122.87739 SPE/0.0 Spencer Creek 17110018000460 11.0 47.74581 -122.87588 TUR/0.0 Turner Creek 17110018000484 0.9 47.72282 -122.88549 JAK/0.0 Jakeway Creek 47.82871 -122.85911 HC0005 47.70592 -122.89569 HC0006 47.70606 -122.89544 HC0037 47.70863 -122.89031 HC0038 47.70852 -122.89049 HC0039 47.70854 -122.89070 HC0040 47.70846 -122.89074 HC0041 47.70835 -122.89100 HC0042 47.70814 -122.89168 HC0043 47.70813 -122.89174 HC0044 47.70779 -122.89233 HC0045 47.70806 -122.89178 HC0079 Turner Creek 47.72274 -122.88499 HC0080 47.72180 -122.88491 HC0081 47.72076 -122.88482 HC0082 47.72047 -122.88487 HC0083 47.72031 -122.88493 HC0084 47.71947 -122.88519 HC0085 47.71892 -122.88541 HC0086 47.71882 -122.88547

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ HC0087 47.71786 -122.88587 HC0088 47.71703 -122.88594 HC0089 47.71503 -122.88620 HC0100 47.70873 -122.88993 HC0101 47.70889 -122.88977 HC0208 47.72346 -122.88461 HC0209 47.72364 -122.88462 HC0210 47.72482 -122.88449 HC0211 47.72505 -122.88446 HC0212 47.72541 -122.88451 HC0213 47.72555 -122.88453 HC0214 47.72619 -122.88446 HC0215 47.72647 -122.88428 HC0216 47.72671 -122.88446 HC0217 47.72681 -122.88447 HC0222 47.70297 -122.89557 HC0224 Spencer Creek 47.74534 -122.87533 HC0225 47.73958 -122.87537 HC0226 Marple Creek 47.73958 -122.87560 HC0227 47.73859 -122.87575 HC0228 47.73286 -122.87844 HC0229 47.73313 -122.87998 HC0245 47.74513 -122.86379 HC0246 47.74346 -122.86059 HC0247 47.74282 -122.86037 HC0248 47.74083 -122.85906 HC0263 47.74850 -122.85246 HC0264 47.76307 -122.85559 HC0265 47.76412 -122.85864 HC0266 47.76420 -122.85870 HC0267 47.76515 -122.85980 HC0268 47.76536 -122.86005 HC0269 47.76930 -122.86416

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ HC0270 47.80530 -122.84500 HC0271 47.80030 -122.84557 HC0272 47.79390 -122.84942 HC0273 47.79224 -122.84947 HC0274 47.79175 -122.84968 HC0275 47.78402 -122.85181 HC0276 47.78475 -122.85095 HC0277 47.78665 -122.85111 HC0278 47.78967 -122.85047 HC0279 47.78115 -122.83749 HC0280 47.77967 -122.84655 HC0301 47.79817 -122.86918 HC0302 47.79635 -122.86922 HC0303 47.79111 -122.86891 HC0304 47.79035 -122.86864 HC0305 47.78654 -122.86738 HC0307 47.77514 -122.86554 HC0308 47.77032 -122.86462 HC0310 47.76816 -122.86320 HC0311 47.81771 -122.84925 HC0312 47.82392 -122.85044 HC0313 47.82503 -122.85240 HC0314 47.82678 -122.85443 HC0315 47.82751 -122.85510 HC0316 Donovan Creek 47.82814 -122.85851 HC0318 47.82707 -122.85992 HC0319 Little Quilcene River 47.82334 -122.85891 HC0320 47.78284 -122.83292 HC0321 47.78202 -122.83471 HC0322 47.78144 -122.83632 HC0323 47.79309 -122.82204 HC0324 47.80012 -122.81928 HC0325 47.80157 -122.81877

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ HC0326 47.80334 -122.81810 HC0327 47.80348 -122.81808 HC0328 47.80598 -122.81791 HC0329 47.80966 -122.81884 HC0330 47.81350 -122.82156 HC0331 47.81937 -122.82560 HC0332 47.82720 -122.82206 HC0333 47.82964 -122.82167 HC0335 47.84300 -122.81611 HC0336 47.84197 -122.81874 HC0337 47.84150 -122.81845 HC0338 47.84113 -122.81980 HC0339 47.84025 -122.82062 HC0340 47.83859 -122.82140 HC0341 47.83696 -122.82179 HC0342 47.83627 -122.82188 HC0343 47.83499 -122.82179 HC0357 47.80072 -122.86726 HC0358 47.79831 -122.86914 HC0361 47.79643 -122.86994 HC0362 47.84893 -122.81430 HC0363 47.84948 -122.81423 HC0364 47.84966 -122.81399 HC0365 47.85268 -122.81384 HC0366 47.85933 -122.81375 HC0367 Tarboo Creek 47.86052 -122.81376 HC0373 47.81228 -122.87078 HC0411 47.85978 -122.81275 HC0420 47.82993 -122.80312 HC0421 47.82775 -122.79880 HC0422 47.82390 -122.79524 HC0423 47.81056 -122.79429 HC0424 47.79798 -122.78826

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ HC0425 47.79295 -122.78854 HC0426 47.79268 -122.78849 HC0427 47.78465 -122.78961 HC0428 47.78433 -122.78969 HC0429 47.78269 -122.79082 HC0430 47.77968 -122.79245 HC0431 47.77549 -122.79425 HC0432 47.77195 -122.79586 HC0433 47.76684 -122.79616 HC0434 47.75590 -122.80162 HC0435 47.75179 -122.80545 HC0436 47.75105 -122.80621 HC0437 47.74802 -122.80881 HC0438 47.73771 -122.80706 HC0439 47.73658 -122.80674 HC0440 47.73158 -122.80661 HC0441 47.73043 -122.80764 HC0442 47.72679 -122.81268 HC0443 47.72583 -122.81369 HC0444 47.71253 -122.81603 HC0485 47.85672 -122.81412 HC0486 47.85707 -122.81422 HC0487 47.85908 -122.81378 HC0563 47.74651 -122.85301 HC0564 47.74060 -122.85257 HC0565 47.73640 -122.85388 HC0566 47.73613 -122.85605 HC0567 47.73836 -122.85829 HC0598 47.82146 -122.79406 HC0599 47.80348 -122.78627 HC0600 47.80242 -122.78674 HC0601 47.79642 -122.78895 HC0602 47.78723 -122.78960

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Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ HC0603 47.76535 -122.79671 HC0604 47.76142 -122.79966 HC0605 47.75740 -122.79949 HC0606 47.73482 -122.80673 HC0607 47.72118 -122.81129 HC0608 47.72080 -122.81091 HC0609 47.71610 -122.81252 HC0611 47.70480 -122.82703 HC0613 47.71837 -122.88561 HC0614 47.71825 -122.88567 HC0615 47.71773 -122.88594 HC0616 47.76198 -122.85378 HC0618 47.70632 -122.89472 HC0619 47.70621 -122.89479 HC0620 47.70616 -122.89500 HC0621 47.70479 -122.89536 HC0622 47.70368 -122.89509 HC0623 47.70353 -122.89515 HC0624 47.70320 -122.89526 HC0625 47.70225 -122.89502 HC0629 47.82805 -122.86019 HC0630 47.82798 -122.86012 HC0631 47.82719 -122.86101 HC0632 Big Quilcene River 47.81920 -122.86339 HC0643 47.75895 -122.85320 HC0644 47.76090 -122.85251 HC0646 47.76003 -122.85268 HC0657 47.85889 -122.81461 HC0660 47.85735 -122.80823 HC0661 47.85713 -122.80783 HC0662 47.85650 -122.80752 HC0663 47.85570 -122.80650 HC0664 47.85535 -122.80577

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ HC0665 47.85498 -122.80585 HC0666 47.85490 -122.80570 HC0667 47.85463 -122.80575 HC0668 47.85445 -122.80578 HC0669 47.85418 -122.80573 HC0670 47.85385 -122.80540 HC0671 47.85372 -122.80527 HC0672 47.85353 -122.80500 HC0673 47.85335 -122.80490 HC0674 47.85217 -122.80463 HC0675 47.85120 -122.80397 HC0676 47.85102 -122.80403 HC0677 47.85073 -122.80395 HC0678 47.85042 -122.80390 HC0679 47.85038 -122.80393 HC0680 47.84997 -122.80410 HC0681 47.84955 -122.80433 HC0682 47.84898 -122.80473 HC0683 47.84818 -122.80503 HC0684 47.84790 -122.80558 HC0685 47.84572 -122.80553 HC0686 47.84538 -122.80437 HC0687 47.84500 -122.80423 HC0688 47.84452 -122.80438 HC0689 47.84370 -122.80420 HC0690 47.84287 -122.80332 HC0691 47.84230 -122.80352 HC0692 47.84168 -122.80365 HC0693 47.84138 -122.80363 HC0694 47.84075 -122.80443 HC0695 47.83924 -122.80452 HC0696 47.83667 -122.80430 HC0697 47.83650 -122.80435

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ HC0698 47.83564 -122.80377 HC0699 47.83437 -122.80393 HC0700 47.83195 -122.80377 HC0701 47.83548 -122.80403 HC0789 47.71311 -122.81510 HC0825 47.74539 -122.87515 HC0826 47.74200 -122.86013 HC0829 47.80104 -122.86770 HC0830 47.80428 -122.86838 HC0831 Indian George Creek 47.80918 -122.87068 HC0832 Indian George Creek 47.80885 -122.87099 HC0833 47.80952 -122.87144 HC0834 47.81017 -122.87178 HC0835 Big Quilcene River 47.81850 -122.86037 HC0837 47.82820 -122.86035 HC0920 Jakeway Creek 47.82877 -122.85899 HC0921 Donovan Creek 47.82889 -122.85909 HC0926 Spencer Creek 47.74586 -122.87588 HC0220 47.69463 -122.89793 HC0957 47.81408 -122.87240 HC0966 Indian George Creek 47.80693 -122.86987 HC0967 47.80741 -122.87083 HC0968 Indian George Creek 47.80670 -122.87062

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Table 15, Stream Monitoring Stations, Quilcene-Dabob Bay Name Waterbody NHD_Reachcode NHDReachMeasure Lat Long_ BQU/0.64 Big Quilcene River 17110018000074 55.7 47.818821 -122.87132 BQU/2.8 Big Quilcene River 47.81075 -122.871312 Camp Discovery CAM/0.1 Creek 17110018000390 79.1 47.810521 -122.790598 CEM/0.6 Cemetery Drain 47.82581 -122.87566 DON/0.2 Donovan Creek 17110018004445 4.8 47.83052 -122.860146 DON/1.8 Donovan Creek 17110018004445 47.0 47.849724 -122.86064 DVT/0.1 Unnamed 47.830453 -122.864424 Indian George IND/0.2 Creek 17110018007836 7.0 47.807539 -122.870648 JAK/0.0 Jakeway Creek 47.828772 -122.858985 LQU/0.1 Little Quilcene River 17110018000060 8.4 47.82783 -122.86462 LQU/0.8 Little Quilcene River 17110018000060 94.5 47.829945 -122.87511 MAR/0.1 Marple Creek 17110018000466 27.6 47.739916 -122.879175 SPE/0.0 Spencer Creek 17110018000460 11.0 47.745813 -122.875881 SPE/2.1 Spencer Creek 17110018000456 36 47.754821 -122.894226 TAR/0.9 Tarboo Creek 17110018000052 83.6 47.869457 -122.818020 TAR/4.2 Tarboo Creek 17110018000054 99.0 47.915971 -122.825848 TUR/0.0 Turner Creek 17110018000484 0.9 47.722817 -122.885493

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Table 16, Marine Monitoring Stations, Quilcene-Dabob Bay WSSNUM GANAME Station Lat WGS84 Long WGS84 97 Dabob Bay 097 -122.75187498900 48.92857583800 99 Dabob Bay 099 -122.49058461300 47.52825579300 107 Dabob Bay 107 -122.36575458200 47.47862580400 109 Dabob Bay 109 -122.94258493400 48.57943580000 112 Dabob Bay 112 -122.93140493300 48.58143580100 294 Dabob Bay 294 -122.40559457100 47.40062579500 295 Dabob Bay 295 -122.42935457500 47.40335579200 296 Dabob Bay 296 -122.43707457800 47.40804579200 297 Dabob Bay 297 -122.43058457800 47.41542579300 131 Hood Canal #3 131 -122.82960492400 48.61533581500 134 Hood Canal #3 134 -122.63620460600 47.38639577100 114 Quilcene Bay 114 -122.93813493500 48.58489580100 115 Quilcene Bay 115 -123.81481463900 46.67226561200 116 Quilcene Bay 116 -123.83377464700 46.70209561200 123 Quilcene Bay 123 -123.91701466000 46.69643560400 312 Quilcene Bay 312 -122.45373456600 47.34384578700

Table 17, Sample frequency and # of samples Shoreline Parameter # of stations # of sampling events Total # of samples Monitoring Fecal Coliform 250 4 1,000 Ammonia 40 4 160 N+N 40 4 160 TKN 40 4 160 Total P 40 4 160 Marine Monitoring Fecal Coliform 16 12 192 Ammonia 8 12 96 N+N 8 12 96 TKN 8 12 96 Total P 8 12 96 Stream Monitoring Fecal Coliform 17 12 204

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016

Table 18, Expected range of results Matrix Parameter Min Max GMV Standard (CFU/100ml) Deviation Freshwater Fecal Coliform 2 CFU/100ml 2010 CFU/100ml 25.5 456.6 Marine Water Fecal Coliform 1.7 CFU/100ml 110 CFU/100ml 2.4 7.1 Freshwater Nitrate+Nitrite 0.02 mg/L 0.4 mg/L 0.09 0.11 Nitrogen (Estimates below are based on lab method detection capabilities.) Freshwater Ammonia- 0.01 mg/L 5 mg/L NA NA Nitrogen Marine Water Ammonia- 0.01 mg/L 5 mg/L NA NA Nitrogen Marine Water Nitrate+Nitrite 0.01 mg/L 5 mg/L NA NA Nitrogen Freshwater Total Kjeldahl 0.2 mg/L 5 mg/L NA NA Nitrogen Marine Water Total Kjeldahl 0.4 mg/L 5 mg/L NA NA Nitrogen Freshwater Total 0.01 mg/L 5 mg/L NA NA Phosphorous-P Marine Water Total 0.01 mg/L 5 mg/L NA NA Phosphorous-P

Table 19, Reporting Requirements Responsible party Product Delivered to Due date Lab Results spreadsheets JCPH 1 week after analysis JCPH Data in EIM spreadsheet EIM Annually, in December JCPH Final report draft Ecology 11/30/2018

Table 20, Entering Non-detects and TNTC data in EIM Lab results What to enter in EIM Example Lab Result Result Result Result reported Reported Detection Detection Data value Value Limit Limit Type Qualifier Fecal coliform non-detect no < 1 1 1 MDL U dilution Fecal coliform non-detect 1:2 < 2 2 2 MDL U dilution Fecal coliform non-detect 1:10 < 10 10 10 MDL U dilution Fecal coliform TNTC no dilution > 200 200 1 MDL E Fecal coliform TNTC 1:2 dilution > 400 400 2 MDL E Fecal coliform TNTC 1:10 dilution > 2,000 2000 10 MDL E

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 18.0 Appendices

A. Chain-of-Custody Form

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 B. Glossary, Acronyms and Abbreviations, Units of Measurement Quality Assurance Glossary Accreditation - A certification process for laboratories, designed to evaluate and document a lab’s ability to perform analytical methods and produce acceptable data. For Ecology, it is “Formal recognition by (Ecology)…that an environmental laboratory is capable of producing accurate analytical data.” [WAC 173-50-040] (Kammin, 2010)

Accuracy - the degree to which a measured value agrees with the true value of the measured property. USEPA recommends that this term not be used, and that the terms precision and bias be used to convey the information associated with the term accuracy. (USGS, 1998)

Analyte - An element, ion, compound, or chemical moiety (pH, alkalinity) which is to be determined. The definition can be expanded to include organisms, e. g. fecal coliform, Klebsiella, etc. (Kammin, 2010)

Bias - The difference between the population mean and the true value. Bias usually describes a systematic difference reproducible over time, and is characteristic of both the measurement system, and the analyte(s) being measured. Bias is a commonly used data quality indicator (DQI). (Kammin, 2010; Ecology, 2004)

Blank - A synthetic sample, free of the analyte(s) of interest. For example, in water analysis, pure water is used for the blank. In chemical analysis, a blank is used to estimate the analytical response to all factors other than the analyte in the sample. In general, blanks are used to assess possible contamination or inadvertent introduction of analyte during various stages of the sampling and analytical process. (USGS, 1998)

Calibration - The process of establishing the relationship between the response of a measurement system and the concentration of the parameter being measured. (Ecology, 2004)

Comparability - The degree to which different methods, data sets and/or decisions agree or can be represented as similar; a data quality indicator. (USEPA, 1997)

Completeness - The amount of valid data obtained from a project compared to the planned amount. Usually expressed as a percentage. A data quality indicator. (USEPA, 1997)

Data Quality Objectives (DQO) - Data Quality Objectives are qualitative and quantitative statements derived from systematic planning processes that clarify study objectives, define the appropriate type of data, and specify tolerable levels of potential decision errors that will be used as the basis for establishing the quality and quantity of data needed to support decisions. (USEPA, 2006)

Dataset - A grouping of samples organized by date, time, analyte, etc. (Kammin, 2010)

Data validation - An analyte-specific and sample-specific process that extends the evaluation of data beyond data verification to determine the usability of a specific data set. It involves a detailed examination of the data package, using both professional judgment, and objective criteria, to determine whether the MQOs for precision, bias, and sensitivity have been met. It may also include an assessment of completeness, representativeness, comparability and integrity, as these criteria relate to the usability

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 of the dataset. Ecology considers four key criteria to determine if data validation has actually occurred. These are:  Use of raw or instrument data for evaluation  Use of third-party assessors  Dataset is complex  Use of EPA Functional Guidelines or equivalent for review

Examples of data types commonly validated would be:  Gas Chromatography (GC)  Gas Chromatography-Mass Spectrometry (GC-MS)  Inductively Coupled Plasma (ICP)

The end result of a formal validation process is a determination of usability that assigns qualifiers to indicate usability status for every measurement result. These qualifiers include:  No qualifier, data is usable for intended purposes  J (or a J variant), data is estimated, may be usable, may be biased high or low  REJ, data is rejected, cannot be used for intended purposes (Kammin, 2010; Ecology, 2004)

Data verification - Examination of a dataset for errors or omissions, and assessment of the Data Quality Indicators related to that dataset for compliance with acceptance criteria (MQO’s). Verification is a detailed quality review of a dataset. (Ecology, 2004)

Detection limit (limit of detection) - The concentration or amount of an analyte which can be determined to a specified level of certainty to be greater than zero. (Ecology, 2004)

Laboratory Control Sample (LCS) - A sample of known composition prepared using contaminant-free water or an inert solid that is spiked with analytes of interest at the midpoint of the calibration curve or at the level of concern. It is prepared and analyzed in the same batch of regular samples using the same sample preparation method, reagents, and analytical methods employed for regular samples. (USEPA, 1997)

Measurement Quality Objectives (MQOs) - Performance or acceptance criteria for individual data quality indicators, usually including precision, bias, sensitivity, completeness, comparability, and representativeness. (USEPA, 2006)

Measurement result - A value obtained by performing the procedure described in a method. (Ecology, 2004)

Method - A formalized group of procedures and techniques for performing an activity (e.g., sampling, chemical analysis, data analysis), systematically presented in the order in which they are to be executed. (EPA, 1997)

Method Detection Limit (MDL) - This definition for detection was first formally advanced in 40CFR 136, October 26, 1984 edition. MDL is defined there as the minimum concentration of an analyte that, in a given matrix and with a specific method, has a 99% probability of being identified, and reported to be greater than zero. (Federal Register, October 26, 1984)

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Percent Relative Standard Deviation (%RSD) - A statistic used to evaluate precision in environmental analysis. It is determined in the following manner: %RSD = (100 * s)/x where s is the sample standard deviation and x is the mean of results from more than two replicate samples (Kammin, 2010)

Parameter - A specified characteristic of a population or sample. Also, an analyte or grouping of analytes. Benzene and nitrate + nitrite are all “parameters” (Kammin, 2010; Ecology, 2004)

Precision - The extent of random variability among replicate measurements of the same property; a data quality indicator. (USGS, 1998)

Quality Assurance (QA) - A set of activities designed to establish and document the reliability and usability of measurement data. (Kammin, 2010)

Quality Assurance Project Plan (QAPP) - A document that describes the objectives of a project, and the processes and activities necessary to develop data that will support those objectives. (Kammin, 2010; Ecology, 2004)

Quality Control (QC) - The routine application of measurement and statistical procedures to assess the accuracy of measurement data. (Ecology, 2004)

Relative Percent Difference (RPD) - RPD is commonly used to evaluate precision. The following formula is used: [Abs(a-b)/((a + b)/2)] * 100 where “Abs()” is absolute value and a and b are results for the two replicate samples. RPD can be used only with 2 values. Percent Relative Standard Deviation is (%RSD) is used if there are results for more than 2 replicate samples (Ecology, 2004).

Replicate samples - two or more samples taken from the environment at the same time and place, using the same protocols. Replicates are used to estimate the random variability of the material sampled. (USGS, 1998)

Sample (field) – A portion of a population (environmental entity) that is measured and assumed to represent the entire population. (USGS, 1998)

Sample (statistical) – A finite part or subset of a statistical population. (USEPA, 1997)

Sensitivity - In general, denotes the rate at which the analytical response (e.g., absorbance, volume, meter reading) varies with the concentration of the parameter being determined. In a specialized sense, it has the same meaning as the detection limit. (Ecology, 2004)

Standard Operating Procedure (SOP) – A document which describes in detail a reproducible and repeatable organized activity. (Kammin, 2010)

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Jefferson County Public Health Quality Assurance Project Plan Quilcene-Dabob Bay PIC, WQC-2016-JeCoPH-00039 May 19, 2016 Acronyms and Abbreviations BEACH - Washington State Beach Environmental Assessment, Communication and Health Program BMP – Best Management Practices WAC - Washington Administrative Code CFS – cubic feet per second CFU, Colony forming units - A quantitative measure of the concentration of bacteria in a water sample, bacterial colonies on laboratory media resulting from filtering and culturing bacteria from a water sample, each colony in the laboratory culture is presumed to have arisen from the multiplication of a single bacterium in the original sample. CREP – Conservation Reserve Enhancement Program Detection limit (DL) - The lowest concentration of a substance in water that can be reproducibly determined by a specific analytical procedure or test method. DOH – Washington Department of Health Ecology – Washington State Department of Ecology EIM – Washington State Department of Ecology Environmental Information Management database El – Elevation et al. – “And others” EPA – U.S. Environmental Protection Agency FC –Fecal Coliform bacteria. GIS – Geographic Information System GMV- Geometric Mean Value. The Nth root of the product of N observations. GPS – Global Positioning System JCCD – Jefferson County Conservation District JCPH – Jefferson County Public Health Marine - Refers to the ocean or to a sea, saltwater. MDL – Method detection limit µ/L (Micrograms per liter) - A concentration unit of chemical constituents in solution; the weight of solute per unit volume of solvent, usually water, one thousand micrograms per liter is equivalent to 1 milligram per liter, this measure is equivalent to parts per billion or ppb. MQO – Measurement Quality Objective MST – Microbial Source Tracking Non-detect – No results detected or measurable. NPDES - National Pollutant Discharge Elimination System permitting program. OSS – On-site Septic Systems QAPP – Quality Assurance Project Plan

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RPD – Relative percent difference RSD – Relative standard deviation SOP – Standard Operating Procedures TMDL – Total Maximum Daily Load TNTC – Too numerous to count WRIA – Water Resource Inventory Areas (WRIA) were formalized under Washington Administrative Code (WAC) 173-500-040 and authorized under the Water Resources Act of 1971, Revised Code of Washington (RCW) 90.54. Ecology was given responsibility for the development and management of these administrative and planning boundaries. WTP – Wastewater Treatment Plant Units of Measurement cfs cubic feet per second mL milliliters ug/L micrograms per liter (parts per billion)

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