DOCSLIB.ORG

Failure to Detect Hantavirus in Vesper Bats in Poland

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Failure to Detect Hantavirus in Vesper Bats in Poland ACTA SCIENTIFIC MICROBIOLOGY (ISSN: 2581-3226) Volume 3 Issue 2 February 2020 Short Report Failure to Detect Hantavirus in Vesper Bats in Poland Satoru Arai1, Fuka Kikuchi1, Janusz Hejduk2, Janusz Markowski2, Received: December 23, 2019 Keiko Tanaka-Taya1, Beata Sikorska3$, Shigeru Morikawa4, Motoi Suzuki1, Paweł P Liberski3#, and Richard Yanagihara5* Published: January 06, 2020 1Infectious Disease Surveillance Center, National Institute of Infectious Diseases, © All rights are reserved by Richard Tokyo, Japan. E-mail: Yanagihara, et al. [email protected], [email protected], [email protected], 2Department of Teacher Training and Biodiversity Studies, Faculty of Biology and [email protected] Environmental Protection, University of Łódź, Łódź, Poland. E-mail: uni.lodz.pl, [email protected] marko@biol. 3Department of Molecular Pathology and Neuropathology, Medical University of Łódź, Łódź, Poland. E-mail: 4Faculty of Veterinary Medicine, Okayama University of Science, Ehime, Japan. [email protected], [email protected] E-mail: 5Departments of Pediatrics and Tropical Medicine, Medical Microbiology and [email protected] Pharmacology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA. E-mail: $Orcid ID: https://orcid.org/0000-0002-6166-0729 [email protected] #Orcid ID: https://orcid.org/0000-0001-6507-4682 *Corresponding Author: Richard Yanagihara, Departments of Pediatrics and Tropical Medicine, Medical Microbiology and Pharmacology, John A. Burns SchoolDOI: 10.31080/ASMI.2020.03.0475 of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii, USA Abstract Objective: We previously reported the co-circulation of genetically distinct hantaviruses (family Hantaviridae ) in shrews and moles detect hantavirus in patagia of vesper bats (order Chiroptera, family Vespertilionidae) from Poland. (order Eulipotyphla, families Soricidae and Talpidae) in central and southeastern Poland. In this exploratory study, we attempted to Methods: RNAlater®-preserved patagia and feces from 88 vesper bats, collected during 2013, 2014 and 2017 in northern, central . Results and Discussion: Despite repeated and exhaustive attempts, using oligonucleotide primers and PCR cycling conditions that and south-central Poland, were analyzed for hantavirus RNA by nested reverse transcription polymerase chain reaction (RT-PCR) including the common noctule (Nyctalus noctula led to the discovery of bat-borne hantaviruses in Asia and Africa, we failed to detect hantavirus RNA in vesper bats from Poland, ), which has recently been shown to harbor a hantavirus in the Czech Republic. More phylogeography of bat-borne hantaviruses. extensive studies of visceral organs from bats in Poland and elsewhere in Europe are warranted to ascertain the genetic diversity and Keywords: Hantaviridae; Vesper Bat; Chiroptera; Poland; RT-PCR Introduction rodents are the principal reservoir hosts [1,2] The discovery of genetically distinct hantaviruses (family America has thoroughly upended the conventional view that Sorex araneus) in Hantaviridae . Earlier, we reported [3], Finland [4] and Hungary [4] ) in multiple species of shrews and moles (order Seewis virus in the Eurasian common shrew ( separated geographic regions in Europe, Asia, Africa and/or North Eulipotyphla, families Soricidae and Talpidae) from widely Switzerland . Subsequently, we reported the co-circulation of Seewis virus in the Eurasian common Citation: Satoru Arai, et al. “Failure to Detect Hantavirus in Vesper Bats in Poland". Acta Scientific Microbiology 3.2 (2020): 07-11. Failure to Detect Hantavirus in Vesper Bats in Poland 08 Sorex minutus), Boginia virus mist nets or found dead, during 2013, 2014 and 2017 (Table 1). Neomys fodiens), and Nova virus in shrew and Eurasian pygmy shrew ( the European mole (Talpa europaea) in central and southeastern in the Eurasian water shrew ( The majority of bats were from Szachownica Cave (Kraków-Wieluń Poland [5,6] in Poland [29] - Upland), which is one of the four largest wintering shelters for bats [7,8] [7] and Slovenia [9]; . Seewis virus has also been detected in Eurasian . Among the 88 bats were four dead common noct common shrews in Germany , Slovakia ules, which were collected during August 2014 at a wind farm in [10]. and Asikkala virus has been reported in the Eurasian pygmy shrew Wicko, a village in the Pomeranian Voivodeship in northern Poland, - in the Czech Republic and Germany approximately 16 km northwest of Lębork and 74 km northwest - [11]. of the regional capital Gdańsk. Live-caught bats were released af In addition to shrews and moles, bats (order Chiroptera) are now served in RNAlater®. The analysis of more than 3,000 frozen, RNAlater® preserved and ter obtaining punch biopsies of patagia and feces, which were pre known to harbor highly divergent lineages of hantaviruses Collection Collection Bat Species Number transcription polymerase chain reaction (RT-PCR) has yielded 13 Site* Date ethanol-fixed tissues from bats representing 11 families by reverse hantaviruses to date in bat species belonging to the suborders Barbastella barbastellus Oct 2017 20 Yinpterochiroptera (families Hipposideridae, Pteropodidae and Cave Szachownica Rhinolophidae) and Yangochiroptera (families Emballonuridae, Eptesicus serotinus Aug 2017 7 Nycteridae and Vespertilionidae). Of the 13 bat-borne hantaviruses, Aug 2017 1 Kurowice Myotis brandtii Jun 2014 1 Łódź [12-15] [14,16], Huángpí virus Aug 2017 2 seven are from Asia: Xuân Sơn virus and Đakrông virus in Vietnam Wicko and Lóngquán virus in China [17] [18], Myotis myotis Oct 2017 4 , Láibīn virus in China and Myanmar Kurowice and Quezon virus in the Philippines [19] , Sarawak virus in Malaysia Cave [20,21], Ponan Szachownica ; and five are from Africa: Myotis mystacinus Oct 2017 1 Cave Mouyassué virus in Côte d’Ivoire and Ethiopia Szachownica Tanzania [unpublished observations], Magboi virus in Sierra virus in Côte d’Ivoire [unpublished observations], Kiwira virus in Myotis nattereri Oct 2017 21 Leone [22] [23]. Thus far, Brno Cave Szachownica virus, detected in the common noctule (Nyctalus noctula) in the Nyctalus noctula Oct 2017 1 , and Makokou virus in Gabon Czech Republic [24], represents the only bat-borne hantavirus Aug 2014 4 Wicko reported in Europe. Because Brno virus, Huángpí virus, Ponan Pipistrellus nathusii Aug 2013 1 Wicko Pipistrellus pipistrellus Aug 2014 1 Wicko virus, Mouyassué virus and Sarawak virus have all been detected Jul 2014 1 archival specimens from a convenience sample of vesper bats Wicko in bats belonging to the family Vespertilionidae, we analyzed Plecotus auritus Brzeziny May 2017 3 from Poland for hantavirus RNA. In addition, because population Wicko Aug 2017 12 genetics studies of bats commonly rely on nonlethal sampling of Oct 2017 6 patagia biopsies stored in ethanol [25-28] Kurowice Cave Szachownica , we decided to ascertain Vespertilio murinus Aug 2014 1 in bats. if patagia would prove useful in screening for hantavirus infection May 2017 1 Wicko Materials and Methods Łódź Specimens Table1: Collection sites and number of vesper bats tested for - hantavirus RNA. limbs and hind limbs, from 88 vesper bats, representing seven gen- Patagia, or the wing membranes or skin folds between the fore o o era and 11 species (Table 1) and designated in the least concern Brzeziny: N 51o o o category of the International Union for Conservation of Nature *Collection site locations: Wicko: N 54 40’18.13, E 17 47’33.99; E 19o o o 47’48.50, E 19 45’27.62; Kurowice: N 51 39’53.38, 42’12.33; Szachownica Cave: N 51 03’15.57, E 18 48’26.94 (IUCN) Red List, were studied. Bats were either captured using Citation: Satoru Arai, et al. “Failure to Detect Hantavirus in Vesper Bats in Poland". Acta Scientific Microbiology 3.2 (2020): 07-11. Failure to Detect Hantavirus in Vesper Bats in Poland 09 RNA extraction, cDNA synthesis and RT-PCR amplification in this study. Thus, apart from the small sample size, patagia and RNAlater®-preserved patagia and feces from 88 vesper bats feces may not be suitable to truly assess hantavirus infection in - bats. Moreover, hantavirus infection is typically focal and not uni- ously described protocols [3-6, 13-15] were analyzed for hantavirus RNA by nested RT-PCR, using previ - it could be that the common noctule population in the particular . Total RNA was extracted, formly distributed within a given host species in time and space. So, using the MagDEA RNA100 Kit (Precision System Science, Mat studo, Japan), and cDNA was synthesized, using the PrimeScript collection site in Wicko is not infected. The other shortcoming is II 1st strand cDNA Synthesis Kit (Takara Bio, Inc., Otsu, Japan) that the common noctules in this study were found dead and only with oligonucleotide primer (OSM55F, 5’–TAGTAGTAGACTCC–3’), RNAlater®-preserved patagia were available for testing. Although hantaviruses [30] designed from conserved 5’–end of the S-, M- and L- segments of hantavirus RNA has been detected in ethanol-fixed liver tissues RNA 2, Virus Refer- . First- and second-round PCR were performed in Host Species Country Posi- Name ence 20-μL reaction mixtures, containing 250 μM dNTP, 2.5 mM MgCl tive [31]. Initial denaturation at 94 °C for 1 U Takara LA Taq polymerase Host Start version (Takara Bio, Inc.) Brno Nyctalus noctula Czech 2/12 24 and 0.25 μM of each primer Republic 2 min was followed by two cycles each of denaturation at 94 °C for Aselliscus Vietnam 1/2 15 s, and elongation at 72 °C for 1 min, then 30 cycles of denaturation 30 s, two-degree step-down annealing from 46 °C to 38 °C for 40 stoliczkanus at 94 °C for 30 s, annealing at 42 °C for 40 s, and elongation at 72 Đakrông Huángpí Pipistrellus abramus China 1/5 17 °C for 1 min, in a Veriti thermalcycler( Applied Biosystems, Foster Taphozous China 1/32 16 City, CA, USA) [19, 27]. melanopogon Láibīn Results and Discussion Taphozous Myanmar 2/13 14 Bats comprise a quarter of the mammalian fauna in Poland, and melanopogon - Lóngquán Rhinolophus China 1/4 17 - monoceros all but two of the 22 bat species in Poland are members of the fam orous bats.
Load more

Recommended publications
  • Karyological Characteristics, Morphological Peculiarities, and a New Distribution Locality for Talpa Davidiana (Mammalia: Soricomorpha) in Turkey
    Turk J Zool 2012; 36(6): 806-813 © TÜBİTAK Research Article doi:10.3906/zoo-1201-13 Karyological characteristics, morphological peculiarities, and a new distribution locality for Talpa davidiana (Mammalia: Soricomorpha) in Turkey Mustafa SÖZEN*, Ferhat MATUR, Faruk ÇOLAK, Sercan IRMAK Department of Biology, Faculty of Arts and Sciences, Bülent Ecevit University, 67100, Zonguldak − TURKEY Received: 17.01.2012 ● Accepted: 17.06.2012 Abstract: Talpa davidiana is the least known species of the genus Talpa, and the karyotype of this species is still unknown. Its distribution records are also very scattered. Th e karyological, cranial, and pelvic characteristics of 2 samples from Kızıldağ in Adana Province were analyzed for the fi rst time. It was determined that T. davidiana has 2n = 34, NF = 66, and NFa = 62. Th e X chromosome was large and metacentric and the Y chromosome was dot-like acrocentric. Th e 2 samples are diff erent from each other, and from previous T. davidiana records, in terms of their lower incisor and premolar numbers. Unique among the T. davidiana samples examined to date, 1 of the samples studied here had 2 premolars on the lower jaw half instead of 3. In contrast to the literature, 1 sample has a europeoidal pelvis, and the other has an intermediate form. T. davidiana has been recorded from 6 localities from the area between Hakkari and Gaziantep provinces in Turkey. Th e Kızıldağ high plateau of Adana was a new distribution locality and the most western for T. davidiana. Th e nearest known locality is Meydanakbes village, and it is almost 160 km away, as the bird fl ies, from Kızıldağ high plateau.
    [Show full text]
  • Article/19/7/12-1820-Techapp1.Pdf)
    LETTERS Novel Bat-borne (S)–segment primer set (outer: OS- found by RT-PCR that used XSV- M55F, 5′-TAGTAGTAGACTCC-3′, specific primers. Hantavirus, and XSV-S6R, 5′-AGITCIGGRTC- Phylogenetic analyses was per- Vietnam CATRTCRTCICC-3′; inner: Cro- formed with maximum-likelihood 2F, 5′-AGYCCIGTIATGRGW- and Bayesian methods, and we used To the Editor: Compelling evi- GTIRTYGG-3′, and JJUVS-1233R, the GTR+I+Γ model of evolution, dence of genetically distinct hantavi- 5′-TCACCMAGRTGRAAGTGRT- as selected by the hierarchical like- ruses (family Bunyaviridae) in multi- CIAC-3. The bat was captured dur- lihood-ratio test in MrModel-test ple species of shrews and moles (order ing July 2012 in Xuan Son National version 2.3 and jModelTest version Soricomorpha, families Soricidae and Park, a nature reserve in Thanh Sơn 0.1 (10), partitioned by codon po- Talpidae) across 4 continents (1–7) District, Phu Tho Province, ≈100 sition. Results indicated 4 distinct suggests that soricomorphs, rather km west of Hanoi (21°07′26.75′N, phylogroups, with XSV sharing a than rodents (order Rodentia, families 104°57′29.98′′E). common ancestry with MGBV (Fig- Muridae and Cricetidae), might be the For confirmation, RNA extrac- ure). Similar topologies, supported primordial hosts (6,7). Recently, the tion and RT-PCR were performed in- by high bootstrap (>70%) and poste- host range of hantaviruses has been dependently in a laboratory in which rior node (>0.70) probabilities, were further expanded by the discovery that hantaviruses had never been handled. consistently derived when various insectivorous bats (order Chiroptera) After initial detection, the L-segment algorithms and different taxa and also serve as reservoirs (8,9).
    [Show full text]
  • Checklist of Rodents and Insectivores of the Mordovia, Russia
    ZooKeys 1004: 129–139 (2020) A peer-reviewed open-access journal doi: 10.3897/zookeys.1004.57359 RESEARCH ARTICLE https://zookeys.pensoft.net Launched to accelerate biodiversity research Checklist of rodents and insectivores of the Mordovia, Russia Alexey V. Andreychev1, Vyacheslav A. Kuznetsov1 1 Department of Zoology, National Research Mordovia State University, Bolshevistskaya Street, 68. 430005, Saransk, Russia Corresponding author: Alexey V. Andreychev ([email protected]) Academic editor: R. López-Antoñanzas | Received 7 August 2020 | Accepted 18 November 2020 | Published 16 December 2020 http://zoobank.org/C127F895-B27D-482E-AD2E-D8E4BDB9F332 Citation: Andreychev AV, Kuznetsov VA (2020) Checklist of rodents and insectivores of the Mordovia, Russia. ZooKeys 1004: 129–139. https://doi.org/10.3897/zookeys.1004.57359 Abstract A list of 40 species is presented of the rodents and insectivores collected during a 15-year period from the Republic of Mordovia. The dataset contains more than 24,000 records of rodent and insectivore species from 23 districts, including Saransk. A major part of the data set was obtained during expedition research and at the biological station. The work is based on the materials of our surveys of rodents and insectivo- rous mammals conducted in Mordovia using both trap lines and pitfall arrays using traditional methods. Keywords Insectivores, Mordovia, rodents, spatial distribution Introduction There is a need to review the species composition of rodents and insectivores in all regions of Russia, and the work by Tovpinets et al. (2020) on the Crimean Peninsula serves as an example of such research. Studies of rodent and insectivore diversity and distribution have a long history, but there are no lists for many regions of Russia of Copyright A.V.
    [Show full text]
  • Molecular Phylogeny of Mobatviruses (Hantaviridae) in Myanmar and Vietnam
    viruses Article Molecular Phylogeny of Mobatviruses (Hantaviridae) in Myanmar and Vietnam Satoru Arai 1, Fuka Kikuchi 1,2, Saw Bawm 3 , Nguyễn Trường Sơn 4,5, Kyaw San Lin 6, Vương Tân Tú 4,5, Keita Aoki 1,7, Kimiyuki Tsuchiya 8, Keiko Tanaka-Taya 1, Shigeru Morikawa 9, Kazunori Oishi 1 and Richard Yanagihara 10,* 1 Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; [email protected] (S.A.); [email protected] (F.K.); [email protected] (K.A.); [email protected] (K.T.-T.); [email protected] (K.O.) 2 Department of Chemistry, Faculty of Science, Tokyo University of Science, Tokyo 162-8601, Japan 3 Department of Pharmacology and Parasitology, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, Myanmar; [email protected] 4 Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology, Hanoi, Vietnam; [email protected] (N.T.S.); [email protected] (V.T.T.) 5 Graduate University of Science and Technology, Vietnam Academy of Science and Technology, Hanoi, Vietnam 6 Department of Aquaculture and Aquatic Disease, University of Veterinary Science, Yezin, Nay Pyi Taw 15013, Myanmar; [email protected] 7 Department of Liberal Arts, Faculty of Science, Tokyo University of Science, Tokyo 162-8601, Japan 8 Laboratory of Bioresources, Applied Biology Co., Ltd., Tokyo 107-0062, Japan; [email protected] 9 Department of Veterinary Science, National Institute of Infectious Diseases, Tokyo 162-8640, Japan; [email protected] 10 Pacific Center for Emerging Infectious Diseases Research, John A.
    [Show full text]
  • The Australasian Bat Society Newsletter, Number 31, Nov 2008
    The Australasian Bat Society Newsletter, Number 31, Nov 2008 The Australasian Bat Society Newsletter Number 39 November 2012 ABS Website: http://abs.ausbats.org.au ABS Discussion list - email: [email protected] ISSN 1448-5877 © Copyright The Australasian Bat Society, Inc. (2012) The Australasian Bat Society Newsletter, Number 31, Nov 2008 The Australasian Bat Society Newsletter, Number 39, November 2012 – Instructions for Contributors – The Australasian Bat Society Newsletter will accept contributions under one of the following two sections: Research Papers, and all other articles or notes. There are two deadlines each year: 10th March for the April issue, and 10th October for the November issue. The Editor reserves the right to hold over contributions for subsequent issues of the Newsletter, and meeting the deadline is not a guarantee of immediate publication. Opinions expressed in contributions to the Newsletter are the responsibility of the author, and do not necessarily reflect the views of the Australasian Bat Society, its Executive or members. For consistency, the following guidelines should be followed: Emailed electronic copy of manuscripts or articles, sent as an attachment, is the preferred method of submission. Faxed and hard copy manuscripts will be accepted but reluctantly! Please send all submissions to the Newsletter Editor at the email or postal address below. Electronic copy should be in 11 point Arial font, left and right justified with 16 mm left and right margins. Please use Microsoft Word; any version is acceptable. Manuscripts should be submitted in clear, concise English and free from typographical and spelling errors. Please leave two spaces after each sentence.
    [Show full text]
  • Popo Wa Mbuga Ya Wanyama Ya Tarangire Bats of Tarangire
    Web Version 1 Popo wa Mbuga ya Wanyama ya Tarangire Bats of Tarangire National Park Imetayarishwa na (created by): Bill Stanley & Rebecca Banasiak Utayarishaji na mfadhili (production and support): The Wildlife Conservation Society, The Field Museum of Natural History [[email protected]] [www.fieldmuseum.org/tanzania] Version 1 6/2009 © Field Museum of Natural History, Chicago Photos by: Bill Stanley and Charles A.H. Foley Epomophorus wahlbergi Hipposideros ruber Cardioderma cor Wahlberg's Epauletted Fruit Bat Noack's Leaf-nosed Bat Heart-nosed Bat Lavia frons Taphozous perforatus Nycteris hispida Yellow-winged Bat Egyptian Tomb Bat Hairy Slit-faced Bat Chaerephon pumilus Scotoecus hindei Scotophilus dinganii Little Free-tailed Bat Hinde's Lesser House Bat Yellow-bellied House Bat Neoromicia capensis Neoromicia nanus Neoromicia somalicus Cape Serotine Banana Pipistrelle Somali Serotine Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.....Small paragraph here.
    [Show full text]
  • Species Examined.Xlsx 8:17 PM 5/31/2011
    8:17 PM 5/31/2011 Names Accepted Binomial Name, Family Page Binomial Name, Page Common Name as of 2011, as given in Sperber if different than in Sperber Monotremata 264 Duckbilled Platypus Ornithorhynchus anatinus 264 Marsupialia 266 Slender‐tailed Dunnart Sminthopsis murina 266 Kultarr Antechinomys laniger 268 Gray Four‐eyed Opossum Philander opossum Didelphys opossum 269 (or possibly Virginia Opossum) ( or Didelphis virginiana) Eastern Grey Kangaroo Macropus giganteus 269 Insectivora 272 Elephant Shrew Macroscelides sp. 272 Hedgehog Erinaceus europaeus 273 Eurasian Pygmy Shrew Sorex minutus 274 Eurasian Water Shrew Neomys fodiens 279 Pygmy White Toothed Suncus etruscus Pachyura etrusca 280 (or Etruscan ) Shrew Russian Desman Desmana moschata 280 Chiroptera 281 Greater Flying Fox Pteropus vampyrus Pteropus edulis 281 (Kalong, Kalang) Northern Bat Eptesicus nilssonii Pipistrellus nilssoni 283 Particoloured Bat Vespertilio murinus 285 Xenarthra 287 and Pholidota Armadillos, anteaters and pangolins: Review of the literature only Rodentia 288 European Rabbit Oryctolagus cuniculus 288 Eurasian Red Squirrel Sciurus vulgaris 293 Eurasian Beaver Castor fiber 294 Agile Kangaroo Rats Dipodomys agilis Perodipus agilis 296 Fresno Kangaroo Rat Dipodomys nitratoides exilis Dipodomys meriami exilis 297 Lesser Egyptian Jerboa Jaculus jaculus 298 Field (or Short‐tailed) Vole Microtus agrestis 299 Bank Vole Myodes glareolus Evotomys glareolus 303 European (or Northern) Water Arvicola terrestris 303 Vole Black Rat Rattus Rattus Epimys rattus 303 House Mouse
    [Show full text]
  • Talpa Aquitania Nov
    NOTES DOI : 10.4267/2042/58283 PRELIMINARY NOTE: TALPA AQUITANIA NOV. SP. (TALPIDAE, SORICOMORPHA) A NEW MOLE SPECIES FROM SOUTHWEST FRANCE AND NORTH SPAIN NOTE PRÉLIMINAIRE: TALPA AQUITANIA NOV. SP. (TALPIDAE, SORICOMORPHA) UNE NOUVELLE ESPÈCE DE TAUPE DU SUD-OUEST DE LA FRANCE ET DU NORD DE L’ESPAGNE Par Violaine NICOLAS(1), Jessica MARTÍNEZ-VARGAS(2), Jean-Pierre HUGOT(1) (Note présentée par Jean-Pierre Hugot le 11 Février 2016, Manuscrit accepté le 8 Février 2016) ABSTRACT A mtDNA based study of the population genetics of moles recently captured in France allowed us to discover a new species, Talpa aquitania nov. sp. We are giving here a preliminary description of the new species. Its distribution covers an area lying south and west of the course of the Loire river in France and beyond the Pyrenees, a part of Northern Spain. Key words: mole, Talpa aquitania nov. sp., mtDNA, France, Spain. RÉSUMÉ Une étude, basée sur le mtDNA, de la génétique des populations de taupes récemment capturées en France nous a permis de découvrir une espèce nouvelle, Talpa aquitania nov. sp. Nous donnons ici une description préliminaire de la nouvelle espèce et de sa distribution. Cette dernière couvre une région se situant au sud et à l’ouest du cours de la Loire et, au-delà des Pyrénées, dans le nord de la péninsule ibérique. Mots clefs : taupe, Talpa aquitania nov. sp., mtDNA, France, Espagne. INTRODUCTION MATERIAL AND METHODS From March 2012 to March 2015, moles were collected in The field collection numbers, name of the collectors, localities different localities in France for which we obtained at of collection and measurements of the specimens are given in least partial mtDNA sequences.
    [Show full text]
  • Index of Handbook of the Mammals of the World. Vol. 9. Bats
    Index of Handbook of the Mammals of the World. Vol. 9. Bats A agnella, Kerivoula 901 Anchieta’s Bat 814 aquilus, Glischropus 763 Aba Leaf-nosed Bat 247 aladdin, Pipistrellus pipistrellus 771 Anchieta’s Broad-faced Fruit Bat 94 aquilus, Platyrrhinus 567 Aba Roundleaf Bat 247 alascensis, Myotis lucifugus 927 Anchieta’s Pipistrelle 814 Arabian Barbastelle 861 abae, Hipposideros 247 alaschanicus, Hypsugo 810 anchietae, Plerotes 94 Arabian Horseshoe Bat 296 abae, Rhinolophus fumigatus 290 Alashanian Pipistrelle 810 ancricola, Myotis 957 Arabian Mouse-tailed Bat 164, 170, 176 abbotti, Myotis hasseltii 970 alba, Ectophylla 466, 480, 569 Andaman Horseshoe Bat 314 Arabian Pipistrelle 810 abditum, Megaderma spasma 191 albatus, Myopterus daubentonii 663 Andaman Intermediate Horseshoe Arabian Trident Bat 229 Abo Bat 725, 832 Alberico’s Broad-nosed Bat 565 Bat 321 Arabian Trident Leaf-nosed Bat 229 Abo Butterfly Bat 725, 832 albericoi, Platyrrhinus 565 andamanensis, Rhinolophus 321 arabica, Asellia 229 abramus, Pipistrellus 777 albescens, Myotis 940 Andean Fruit Bat 547 arabicus, Hypsugo 810 abrasus, Cynomops 604, 640 albicollis, Megaerops 64 Andersen’s Bare-backed Fruit Bat 109 arabicus, Rousettus aegyptiacus 87 Abruzzi’s Wrinkle-lipped Bat 645 albipinnis, Taphozous longimanus 353 Andersen’s Flying Fox 158 arabium, Rhinopoma cystops 176 Abyssinian Horseshoe Bat 290 albiventer, Nyctimene 36, 118 Andersen’s Fruit-eating Bat 578 Arafura Large-footed Bat 969 Acerodon albiventris, Noctilio 405, 411 Andersen’s Leaf-nosed Bat 254 Arata Yellow-shouldered Bat 543 Sulawesi 134 albofuscus, Scotoecus 762 Andersen’s Little Fruit-eating Bat 578 Arata-Thomas Yellow-shouldered Talaud 134 alboguttata, Glauconycteris 833 Andersen’s Naked-backed Fruit Bat 109 Bat 543 Acerodon 134 albus, Diclidurus 339, 367 Andersen’s Roundleaf Bat 254 aratathomasi, Sturnira 543 Acerodon mackloti (see A.
    [Show full text]
  • Mammalia) from the Miocene of Be³chatów, Poland
    Acta zoologica cracoviensia, 48A(1-2): 71-91, Kraków, 30 June, 2005 Erinaceomorpha and Soricomorpha (Mammalia) from the Miocene of Be³chatów, Poland. IV. Erinaceidae FISCHER VON WALDHEIM, 1817 and Talpidae FISCHER VON WALDHEIM, 1817 Barbara RZEBIK-KOWALSKA Received: 12 Jan., 2005 Accepted for publication: 12 Apr., 2005 RZEBIK-KOWALSKA B. 2005. Erinaceomorpha and Soricomorpha (Mammalia) from the Miocene of Be³chatów, Poland. IV. Erinaceidae FISCHER VON WALDHEIM, 1817 and Tal- pidae FISCHER VON WALDHEIM, 1817. Acta zoologica cracoviensia, 48A(1-2): 71-91. Abstract. Very scarce remains of Erinaceidae and Talpidae have been found in three dif- ferent layers of Miocene sediments in Be³chatów in central Poland. Talpidae gen. et sp. in- det. and Desmanella cf. engesseri were stated in horizon C, dated from the Middle (MN4 or MN4/MN5) Miocene, Lanthanotherium aff. sansaniense, Mygalea cf. antiqua, Talpa minuta,“Scaptonyx”cf. edwardsi and Desmanella engesseri in horizon B, dated from the Middle (MN5 or MN5/MN6) Miocene and ?Talpa minuta, Desmanella cf. stehlini and Talpidae gen. et sp. indet. in horizon A, dated from the late Middle (MN7+8) or Mid- dle/Late (MN7+8/MN9) Miocene boundary. The remains are described and illustrated and their systematic position is discussed. Key words: fossil mammals, Insectivora, Erinaceidae and Talpidae, Miocene, Poland. Barbara RZEBIK-KOWALSKA, Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, S³awkowska 17, 31-016 Kraków, Poland. E-mail: [email protected] I. INTRODUCTION The present paper is the fourth part of a series of studies on the remains of Erinaceomorpha and Soricomorpha from the Miocene locality of Be³chatów in central Poland.
    [Show full text]
  • A Synopsis of Records of Myxozoan Parasites (Cnidaria
    © Institute of Parasitology, Biology Centre CAS Folia Parasitologica 2016, 63: 021 doi: 10.14411/fp.2016.021 http://folia.paru.cas.cz Research Note A synopsis of records of myxozoan parasites (Cnidaria: Myxozoa) from shrews, with additional data on Soricimyxum fegati from common shrew Sorex araneus in Hungary and pygmy shrew Sorex minutus in Slovakia Csaba Székely1, Stephen D. Atkinson2, Kálmán Molnár1, László Egyed1, András Gubányi3 and Gábor Cech1 1 Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, Budapest, Hungary; 2 Department of Microbiology, Oregon State University, Corvallis, Oregon, USA; 3 Department of Zoology, Hungarian Natural History Museum, Budapest Abstract: Myxozoans (Cnidaria: Myxozoa) are almost exclusively endoparasites of aquatic vertebrates and invertebrates, with the notable exception being two species of Soricimyxum Prunescu, Prunescu, Pucek et Lom, 2007 described from terrestrial shrews (Sori- cidae) in central Europe. Myxospores of the two parasites are morphologically indistinguishable, but have SSU rDNA sequences that differ by about 4%. Herein, we report additional molecular and histology data from Soricimyxum fegati Prunescu, Prunescu, Pucek et Lom, 2007 from common shrew (Sorex araneus Linnaeus) from Hungary, and add a new geographic record for S. fegati in pygmy shrew (Sorex minutus Linnaeus) from Slovakia. A limited survey of shrews from the northern United States, Blarina brevicauda Say and Sorex sp. from New York, and Sorex spp. from Oregon, did not discover any infections, which is in stark contrast to the relatively high infection rates (up to 66%) in European shrew populations. We also provide a summary and discussion of literature records of spe- cies of Soricimyxum and a host survey.
    [Show full text]
  • Ecological and Faunal Complexes of Insectivorous Mammals of the Republic of Mordovia, Russia
    BIODIVERSITAS ISSN: 1412-033X Volume 21, Number 7, July 2020 E-ISSN: 2085-4722 Pages: 3344-3349 DOI: 10.13057/biodiv/d210758 Short communication: Ecological and faunal complexes of insectivorous mammals of the Republic of Mordovia, Russia ALEXEY ANDREYCHEV♥ Department of Zoology, National Research Mordovia State University. Bolshevistskaya street, 68, Saransk 430005, Russia. Tel./fax.: +7-342-322637, email: [email protected] Manuscript received: 30 March 2020. Revision accepted: 27 June 2020. Abstract. Andreychev A. 2020. Short communication: Ecological and faunal complexes of insectivorous mammals of the Republic of Mordovia, Russia. Biodiversitas 21: 3344-3349. In this study, reports that the species composition and occurrence of species in geo- ecological districts are not the same. 12 insectivorous mammals species have been recorded in the territory of Mordovia. The largest number of species in the region belongs to those living in coniferous and broad-leaved forests (42%). In the second place in terms of representation are species widely distributed in several natural areas (33%). They are slightly inferior to the types of taiga fauna (25%). For each geo- ecological district, the features of the rodent fauna are given and rare species are identified. The forest-steppe region of Mordovia is compared in insectivorous mammals fauna with other regions of Russia with different typical faunal complexes. Keywords: Habitat, insectivorous mammals, population, Russia, species INTRODUCTION In this paper present updated information on the fauna
    [Show full text]