Diabetes Page 2 of 61 Adipose recruitment and activation of plasmacytoid dendritic cells fuel metaflammation Amrit Raj Ghosh1, Roopkatha Bhattacharya1, Shamik Bhattacharya1, Titli Nargis2, Oindrila Rahaman1, Pritam Duttagupta1, Deblina Raychaudhuri1, Chinky Shiu Chen Liu1, Shounak Roy1, Parasar Ghosh3, Shashi Khanna4, Tamonas Chaudhuri4, Om Tantia4, Stefan Haak5, Santu Bandyopadhyay1, Satinath Mukhopadhyay6, Partha Chakrabarti2 and Dipyaman Ganguly1*. Divisions of 1Cancer Biology & Inflammatory Disorders and 2Cell Biology & Physiology, CSIR- Indian Institute of Chemical Biology, Kolkata, India; 4ILS Hospitals, Kolkata, India; 5Zentrum Allergie & Umwelt (ZAUM), Technical University of Munich and Helmholtz Centre Munich, Munich, Germany; Departments of 3Rheumatology and 6Endocrinology, Institute of Postgraduate Medical Education & Research, Kolkata, India. *Corresponding author: Dipyaman Ganguly, Division of Cancer Biology & Inflammatory Disorders, CSIR-Indian Institute of Chemical Biology, 4 Raja S C Mullick Road, Jadavpur, Kolkata, West Bengal, India, 700032. Phone: 91 33 24730492 Fax: 91 33 2473 5197 Email:
[email protected] Running title: PDCs and type I interferons in metaflammation Word count (Main text): 5521 Figures: 7, Table: 1 1 Diabetes Publish Ahead of Print, published online August 25, 2016 Page 3 of 61 Diabetes ABSTRACT In obese individuals the visceral adipose tissue (VAT) becomes seat of chronic low grade inflammation (metaflammation). But the mechanistic link between increased adiposity and metaflammation remains largely