______ WelcomePROGRAM Letter Dear Attendee of NEMB2018: On behalf of the organizers, we would like to welcome you to Los Angeles to the 6th American Society for Mechanical Engineers (ASME) NanoEngineering in Medicine and Biology Conference (NEMB 2018). At this meeting, you will have the opportunity to discuss the most recent advances in nanobiotechnology and nanomedicine with luminaries and pioneers of the field. Whether it’s a tutorial, plenary, keynote and theme presentation, a poster presentation, or a social event, we believe that you will enjoy these exciting elements of the conference. We hope you will benefit from the academic and industrial breadth of the meeting. Applications of Nanoengineering for medicine and biology are having a dramatic impact on a myriad of healthcare needs, product development and biomedical research. These include nanoparticle-based imaging probes and drug/gene carriers for new and improved disease diagnostics and therapeutics, bio-inspired nanomaterials and nanostructures, and novel Nanoengineering tools for biologicalConference and medical applications. | August These 21-24, frontiers 2018 of engineering, biology and medicine at the micro- and nano-scale will be highlighted at NEMB 2018. In addition, work contributing to completely new fundamental understandingExhibition of |biological August processes 22-23, and2018 phenomenon at the nanoscale will also be presented. Together these fundamental and applied aspects of the meeting provide the basis for solving grand challenge problems in cancer, infectious disease, cardiovascular, neurological diseases and global health. NanoengineersOMNI and Los nanoscientists Angeles areHotel indeed, changing the world and are having a profound impact on medicine and biology! California Plaza, We would like to acknowledge support for the conference from the National Science Foundation, Purdue University, WashingtonLos Angeles University ,at CA St. Louis, University of California, Irvine, University of Minnesota, and many generous funders acknowledged on our website and in our program. We would also like to acknowledge and highlight the many volunteers who helped assemble the scientific program for this conference especially our Steering Committee (Professors Abe Lee, Bumsoo Han, John Bischof, Guy Genin, Gang Bao), the Tutorial Speakers and Participants, Track Chairs, and Poster Awards committee (led by Zhenpeng Qin and Masaru Rao). You will see that amongst the Plenary and Keynote Speakers, Track Chairs and other attendees many are leading figures in the field. In addition to stimulating scientific and technical discussions, there will be ample opportunities for networking, especially for young researchers to mingle with senior investigators. Showcasing cutting edge research in Nanobioengineering with broad participation constitutes the heart of this meeting, as evident by how the talks, sessions, and tracks are constructed in the technical program. Finally, working hard behind the scenes is a devoted staff of technical assistants from ASME. The latter includes lead organizer extraordinaire Christine Reilley, Program Manager – Kanupriya Parasher and Webtool Coordinator – Laraine Lee. We wish you a productive and enjoyable stay in Los Angeles. Please stay tuned for information about the next NEMB conference! Abe Lee Bumsoo Han Conference Chair Technical Program Chair NEMB 2018 XACTTM is a novel delivery technology that aims to overcome obstacles to the effec- tive utilization of CNTs in biological systems. Here, we demonstrate that chemical- TUESDAY, AUGUST, 21 ly-functionalized, purified, discrete CNTs (dCNTs) yield bio-compatibilized and high- ly-stable delivery vehicles with predictable properties and performance. In addition, we provide evidence that in vivo biodistribution of dCNTs is tunable by altering physical and chemical properties. TRACK 1 Stability and nontoxicity of dCNTs are essential for successful utilization in biological Nano/micro therapeutics and drug delivery systems. Long-term colloidal stability of dCNTs was evaluated by analyzing zeta po- tential (-40 to -50 mV) and physiological compatibility was optimized for ionic, systems high-serum environments. Cytotoxicity of dCNTs was evaluated in several murine and human cell-types, including prostate and myeloma cancer cell lines, as well as normal MSCs. Critically, dCNTs were found not found to elicit cytotoxicity in therapeutical- Track Organizer: Xiaoming He, University of Maryland, College Park, MD, Unit- ly-relevant concentrations. In order to investigate the transmembrane transport poten- ed States tial, LNCaP cells were treated with dCNTs loaded with KLAKLAK, a pro-apoptotic Track Co-Organizer: Seungpyo Hong, University of Wisconsin-Madison, Madi- peptide incapable of diffusion across the cellular plasma membrane. Following 72-hour son, WI, United States incubation, cells treated with peptide alone or unloaded dCNTs remained viable, while no cells remained in groups treated with dCNTs loaded with the apoptotic ligand. 1-1 These findings demonstrated feasibly for in vivo drug delivery studies, as they estab- lished that dCNT endocytosis is non-cytotoxic and dCNT-loading does not alter biolog- MICRO/NANOTECHNOLOGY FOR THERAPEUTICS DELIVERY ical functionality of payload. an vivo toxicity was investigated in a murine model by Los Angeles, OMNI Hotel, Museum A intravenous administration. dCNTs were well-tolerated and achieved a high MTD of 70 2:00pm - 3:20pm mg/kg. Biodistribution studies were conducted with two unique formulations of liver- and bone-targeting dCNTs using radioisotope labeling. Approximately 60% of injected dCNTs from each formulation trafficked to their physiological target, while other frac- Session Organizer: James Moon, University of Michigan, Ann Arbor, MI, tions demonstrated clearance from various organs. United States Advanced Functional Magnetic Glyconanoparticles for Atherosclerosis We next evaluated the potential of bone-targeting dCNTs for therapeutic delivery in a Detection murine Raji-Burkitt?s lymphoma model. Whole-body bioluminescence imaging re- Keynote. NEMB2018-6139 vealed that, similarly to treatment with free Dox, the group receiving Dox-loaded, bone-targeting dCNTs showed significantly reduced tumor burden. In addition, this group demonstrated increased survival compared to free Dox and untreated controls. Xuefei Huang, Seyedmehdi Hossaini Nasr, Chunqi Qian, Michigan State Typical indications of Dox-induced toxicity, such as rapid weight loss and/or University, East Lansing, MI, United States drug-related mortality, were not observed in mice receiving Dox-loaded dCNTs, con- firming the potential of site-specific delivery to reduce chemotherapy-associated mor- Carbohydrates are ubiquitous in nature. They play important roles in many biological bidities. functions. In this talk, we will present our work in combining biological recognition of a carbohydrate, i.e., hyaluronan (HA), with the properties of magnetic nanoparticles for In summary, XACTTM dCNTs are stable, nontoxic and versatile delivery vehicles, the detection of vascular inflammation and atherosclerosis. featuring a promising capability for targeted delivery to cells, organs and tissues. This Cardiovascular diseases, often associated with inflammation and atherosclerosis, are novel technology offers considerable clinical potential as a therapeutic delivery mecha- the leading cause of death and disability in the world. Despite the significant progress nism for bone and liver diseases, but is also suitable for bio-industrial processes such in recent years, there remain large unmet needs to detect vulnerable atherosclerotic as intracellular gene transport in agricultural engineering. plaques, which are prone to ruptures subsequently causing heart attacks and strokes. CD44 is a cell surface receptor, which has been shown by multiple studies to promote Delivery of Docetaxel to Breast cancer cells Employing Water Soluble atherosclerosis by mediating inflammatory cell recruitment and vascular cell activation. Carbon Nanotubols: Enhanced Anti-neoplastic activity and Improved Moreover, the expression of CD44 is up-regulated more than ten folds at rupture prone Pharmacokinetic Profile vascular sites, thus presenting an attractive target for molecular imaging. HA is a major endogenous ligand of CD44. In order to detect the presence of CD44 in ather- Technical Presentation. NEMB2018-6105 osclerotic plaques, we have synthesized magnetic nanoparticles coated with HA. However, significant inflammatory responses were observed when macrophages were Nagarani Thotakura, Kaisar Raza, Vipin Kumar, Central University of incubated with these nanoparticles. Interestingly, we discovered that engineering of the rajasthan, KISHANGARH, Rajasthan, India shape of the nanoprobes can significantly reduce the inflammatory properties of the probes. The new nanoprobes have high relaxivities, which are suitable contrast agents In the present research, the aim was to synthesize aspartic acid tagged water soluble for magnetic resonance imaging. The results on non-invasive in vivo detection of ath- CNTnols and to deliver docetaxel to cancer cells with enhanced safety and efficacy. erosclerotic plaques in a clinically relevant model of ApoE knockout transgenic mice Various characterization studies like FT-IR and NMR spectroscopy were performed aided by these HA functionalized nanoprobes will be presented. after synthesizing Docetaxel conjugated aspartic acid derivatized CNTnols. Particle size analysis, zeta potential, PDI and