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Type Quorum Sensing System Contributes in the Regulation of Swarming Motility and Antibiosis in a Non-Pathogenic Burkholderia Gladioli Strain

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Type Quorum Sensing System Contributes in the Regulation of Swarming Motility and Antibiosis in a Non-Pathogenic Burkholderia Gladioli Strain SUNDAY 1 MONDAY 2 TUESDAY 3 WEDNESDAY 4 THURSDAY 5 7:00‐9:00 BREAKFAST BREAKFAST BREAKFAST BREAKFAST ORAL SESSION I ORAL SESSION IV ORAL SESSION VI 9:00‐10:40 Chair: Chair: Chair: 10:40‐11:00 COFFEE BREAK COFFEE BREAK COFFEE BREAK SYMPOSIUM DEPARTURE ORAL SESSION II ORAL SESSION VII 11:00‐12:40 Chair: Chair: Chair: REGISTRATION 11:00 – 18:00 12:40‐13:00 COFFEE BREAK COFFEE BREAK COFFEE BREAK PLENARY SESSION V PLENARY SESSION I PLENARY SESSION III VÍCTOR DE LORENZO 13:00‐14:00 GAD FRANKEL VANESSA SPERANDIO Centro Nacional de Imperial College London UT Southwestern USA Biotecnología, CSIC. España 14:00‐16:00 LUNCH LUNCH LUNCH PLENARY SESSION VI OPENING CEREMONY ORAL SESSION III ORAL SESSION V 16:00‐17:00 JAVIER TORRES LOPEZ 17:45‐18:00 Chair: Chair: IMSS PLENARY SESSION IV OPENING TALK PLENARY SESSION II LAURA CAMARENA FRANCISCO BOLÍVAR GISELA STORZ Closing Ceremony 17:00‐18:00 Instituto de Investigaciones IBT‐UNAM NICHD, NIH. Bethesda, USA 17:00‐17:30 Biomédicas, UNAM 18:00‐19:00 Poster Session Poster Session WELCOME COCKTAIL DINNER AND DANCING 18:00‐20:00 Odd Numbers Even Numbers 19:00‐21:00 20:00 h P L E N A R I A S FIFTH MEETING ON BIOCHEMISTRY ANDMOLECULAR BIOLOGY OF BACTERIA OCTOBER 1-5, 2017 EXHACIENDACHAUTLA, PUEBLA, MEXICO Biotecnología y los beneficios de los organismos transgénicos. Dr. Francisco Bolívar Zapata Investigador Emérito. Instituto de Biotecnología, Universidad Nacional Autónoma de México. [email protected] La biotecnología de los organismos y plantas transgénicos se han usado desde hace muchos años para ayudar a contender con diferentes problemáticas y ayudar a producir satisfactores entre ellos están indudablemente nuevos medicamentos. Las plantas transgénica están diseñadas para contender con las plagas de insectos y simultáneamente reducir la cantidad de insecticidas químicos muchos de los cuales son carcinogénicos y recalcitrantes. Este propósito se ha cumplido ya que en Estados Unidos y en otros países se han ido sustituyendo los cultivares tradicionales por los cultivares transgénicos. En Estados Unidos el 90% de sus cultivares son principalmente maíz, algodón y soya entre otros cultivares transgénicos. Sus granjeros han visto las ventajas del uso de cultivares transgénicos al haber dejado la compra de insecticidas químicos, ahorrando recursos económicos y de forma importante, además ya no están expuestos a muchos de los efectos nocivos sobre la a la salud. Se discutirá también sobre la posibilidad de emplear plantas transgénica desarrolladas en México para ayudar contender muchos de los problemas asociados con producción sustentable de alimentos y del impacto hacia el medio ambiente. FIFTH MEETING ON BIOCHEMISTRY ANDMOLECULAR BIOLOGY OF BACTERIA OCTOBER 1-5, 2017 EXHACIENDACHAUTLA, PUEBLA, MEXICO Citrobacterrodentium infection reprogrammemetabolism in intestinal epithelial cells in vivo Cedric Berger, Valerie Crepin, TheodorosRoumeliotis, James Wright, Danielle Carson, Meirav Pevsner-Fischer, EranElinav, Gerald J. Larrouy-Maumus, Jyoti S. Choudhary, Gad Frankel MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Imperial College London, London, United Kingdom Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom Department of Immunology, The Weizmann Institute of Science, Rehovot, Israel The intestinal epithelial cells (IECs) that line the gut form a robust line of host defense against ingested pathogens, acting as a physical barrier and through detection of pathogen-associated molecular patterns (PAMPs) via pattern recognition receptors (PRRs), such as toll-like receptor (TLR) 2 and TLR4. As such, the pathogen - IEC interface constitutes the battle line between the host innate immune system and the pathogen’s counteracting virulence factors. We investigated the impact of infection with the enteric mouse pathogen Citrobacter rodentium (a model organism to study infections with enteropathogenic and enterohemorrhagicEschericha coli (EPEC and EHEC)) on IEC metabolism, using global proteomic and targeted metabolomics and lipidomics. We found that C. rodentium infection results in shut down of mitochondrial ATP production, a switch to aerobic glycolysis and production of phosphocreatine, which mobilises cytosolic energy.Consistently, there was a significant reduction in the levels of host high molecular weight cardiolipins, lipids essential for efficient mitochondrial oxidative phosphorylation. Using bioluminescent reporter strains we show that infection with WT type C. rodentium leads to increased oxygenation of the mucosal surface, supporting C. rodentium oxidative metabolism in vivo and reducing the abundance of obligate anaerobic commensals. Additionally, IECs responded to infection by activating Srebp2 and the cholesterol biosynthetic pathway. Unexpectedly, infected IECs also up-regulated the cholesterol efflux proteins AbcA1, AbcG8 and ApoA1, resulting in higher levels of fecal cholesterol and a bloom of proteobacteria. These results suggest that C. rodentium manipulates host metabolism to evade innate immune responses and establish a favourable gut ecosystem. FIFTH MEETING ON BIOCHEMISTRY ANDMOLECULAR BIOLOGY OF BACTERIA OCTOBER 1-5, 2017 EXHACIENDACHAUTLA, PUEBLA, MEXICO The Hidden Secrets of Small Genes Gisela Storz Division of Molecular and Cellular Biology NICHD, NIH. Bethesda, USA Small RNAs (sRNAs) that base pair with one or more target mRNAs to regulate their translation and stability are critical to stress responses in bacteria. In a number of bacteria, the Hfq chaperone protein facilitates the limited base pairing between the sRNAs and their targets. Initially, it was assumed that Hfq-binding sRNAs are encoded by independent, non-protein coding genes. However, a number of recent observations suggest that this is not the case for many sRNAs, and the distinction between coding and noncoding is becoming increasingly blurred. First, more and more sRNAs derived from the 3’ end of protein coding genes, either through processing of mRNAs or transcription from promoters within coding sequences, are being discovered. For example, the MicL RNA, which represses the synthesis of the most abundant protein in the cell, is transcribed from a promoter located within the coding sequence of the cutCgene. The copper sensitivity phenotype previously ascribed to the inactivation of cutC is actually derived from the loss of MicL. Second, more and more sRNAs thought to function solely as base pairing sRNAs have been found to encode small proteins. For example, the Spot42 RNA, which represses the synthesis of proteins required for the metabolism of non-preferred carbon sources, encodes a 15-amino acid membrane protein. Several of these small proteins modulate the activities or levels of transporters, some encoded by mRNAs that are targets of the corresponding sRNA. FIFTH MEETING ON BIOCHEMISTRY ANDMOLECULAR BIOLOGY OF BACTERIA OCTOBER 1-5, 2017 EXHACIENDACHAUTLA, PUEBLA, MEXICO “You’re hot and you’re cold”: neurotransmitters modulation of bacterial virulence gene expression. Vanessa Sperandio Depts. of Microbiology and Biochemistry UT Southwestern Medical Center Host bacterial associations have a profound impact in health and disease. The gastrointestinal (GI) tract is one of the most prominent sites in the human body where host/microbial associations are paramount. Chemical communication between microbes and their hosts underlies the basis of their associations, andthis inter-kingdom signaling mediates amicable and detrimental interactions. Both the host and microbiota produces cohorts of signalling molecules that can regulate the virulence expression of a pathogen. Epinephrine and/or norepinephrine are sensed by two bacterial adrenergic receptors to activate virulence gene expression in enteric pathogens. Serotonin is a neurotransmitter that is primarily synthesized in the GI tract by enterochromaffin cells and a subset of enteric neurons. The enzyme tryptophan hydroxylase 1 (TpH1) is responsible for the synthesis of serotonin. Serotonin signaling in the intestinal mucosa is terminated by its removal by the serotonin selective reuptake transporter (SERT). Many functional GI disorders are associated with alterations in serotonin signaling, but the effect of serotonin signaling on bacterial-mediated GI disorders remains unknown. We assessed the role of serotonin in the virulence expression of enterohemorrhagic E.coli (EHEC). EHEC has a pathogenicity island, the locus of enterocyte effacement (LEE) that is essential for virulence. Serotonin decreased transcription of the LEE genes through Ler, the major transcriptional activator of the LEE genes. Congruently, the levels of the LEE proteins EspB and EspA were decreased both in whole cell lysates and supernatants upon treatment with serotonin. The LEE genes are necessary for attaching and effacing (AE) lesion formation on epithelial cells, and serotonin also decreased AE lesion formation. To assess the effect of serotonin as a signalling molecule regulating LEE in-vivo studies using SERT knockout mice, which accumulate increased levels of serotonin in the GI tract, were performed. Mice having normal levels serotonin and those with increased levels of serotonin were infected with C.rodentium, which is used as a surrogate model for EHEC infection. Levels of colonization, and survival were performed. The mice with increased levels of serotonin were less susceptible to C.rodentium infection. Conversely, mice had increased levels of infection when the serotonin levels in the GI tract were
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