ISTH 2017 1295--PB CONCLUSIONS RESULTS SUBJECTS& SAMPLES BACKGROUND & AIM • • • • • • • • plasma (PPP) andrich plasma platelet (PRP). Whole bloodwith minimal stasis was collected obtain to poorplatelet collection. (at leastdays), 10 and dietary chilitwo days),least (at prior to Healthy subjects (n=4) avoidedaspirin andantiplateletmedications thromboxane B2 (TXB2) formation. aloneand together, onplateletaggregation,count, platelet and The CAP:DHC proportionsthey are that found indifferent60:40 fruits, i.e., Their effects onaggregation whenpresent intherelative collagen neitherCAP nor DHC appear tohave aninhibitoryeffect on lesser extentwith ADP arachidonic acid (AA) Individually,CAP and DHChave strong inhibitoryeffects on (DHC), are thepungent principles chilli of peppers Capsaicinoids arachidonic acid pathway. may be exploited therapeutic for by dampening benefit plateletactivity via the Further investigations arewarranted todetermine whether these compared to individual not ADP or collagen peppers, produces asignificantly greater inhibitoryeffect on AA The combinationof CAP and DHCintheproportions they arepresentchilli in Fig. 1.Fig. platelet effect 12 Fig aim . 5 . 2 The effectof . μM on Effect count - of this study of this was todetermineeffects the CAP of and DHC, platelet of induced aggregation 4 Contact: CAP

% Platelet Count , are not known. of (normalised Murray Adams Platelet Function andInteractions 100 11 CAP, 70 80 90 count and , including, capsaicin (CAP) and 0 capsaicinoids 300 µg/mL300 AACAP+ 7.5µM DHC µM + 5 µg/mL300 AADHC + 6.5 µM µg/mL300 AACAP µM + 12.5 µg/mL300 AA DHC DHC, over to and 0 - platelet 2 and induced)aggregationplatelet and TXB2 formation, [email protected] - hours their CAP DHC 12.5 µM CAP Buf induced - capsaicinoids Safa Y Almaghrabi on platelet aggregation induced by 300aggregation by platelet induced on the induced aggregationplatelet Synergistic Inhibitory Effect of Capsaicin and ofCapsaicin Effect Inhibitory Synergistic 30 count fer combination of combination 7.5 µM + DHC 5 µM 12.5 µM incubation Ti at m on the Arachidonic of Platelet Acid Pathway Aggregation 3 time 60 . in vitro e

zero) . ( on of m Data CAP platelet i . n Mean 90 are . ) School of Veterinary and Life Sciences, Murdoch University, Perth, Western Australia, Australia. : DHC plateletaggregation,and toa presented ± counts SEM ( 120 7 School of Health Sciences, University of Tasmania, Launceston, Tasmania, Australia . 5 ; : 5 . n= as μM 4 dihydrocapsaicin . per )  , g/mL have cent 1,2 1 , Kiran DK Ahuja AA 3 no . of . In contrast, . - induced (but each n= the Data (C Fig capsaicinoids 4 and . . absence 3 *p< column are . Effects D) 0 presented . 05 and . , of **p< collagen of capsaicinoid DOI: 10.3252/pso.eu.ISTH2017.2017 METHODS CAP, • Thromboxane B2 • • Platelet Counts • • • Platelet Aggregation 0 1 . 001 as , DominicP Geraghty hours. DHC), were added separatelyPRP, to andcount the measured every 15 minfor2 CAP, DHC (individuallyat 12.5 Diagnostics) withPRP adjusted 250 to x10 Platelet counts were determined usinga Laboratories). Aggregationwas recorded for10 minusing an 3.75:2.5 concentration), andincombination ratio ofin a 60:40(CAP:DHC; 7.5:5 CAP and DHCwere investigated individuallyat 12.5 DHC. PRP (225 AA (300 instructions ( combinationwas measured using aELISA kit according tomanufacturer’s absence and presence of12.5 and6.25 Thromboxane B2 release from plateletstreatedwith AA (300 percent - DHC compared (E and (i and . e area . F) , agonist their REFERENCES • ACKNOWLEDGEMENTS induced to 4. 3. 2. 1. μg μM under control This study was funded by: • • μL combination Garces Almaghrabi SY Kosuge Bennett DJ /mL), ADP (5 only) King Abdul Aziz University, Jeddah, Saudi Arabia (SYA). Clifford CraigMedical Research Trust, Tasmania (MJA, DPG, KDKA). ). curve ) toinitiateaggregationin thepresence andabsence CAPof and platelet Abcam (i . . e . Results , - normalised Claver 0 S µM aggregation et et al on et et al ). of are AA . A capsaicinoid et et al Agricultural Agricultural and Biological Chemistry . - the et et al Journal Journal of the Chemical Society C to (A μM aggregation . mean . Poster and Tuesday, July 11 Thrombosis Thrombosis Research . Journal Journal of Agricultural Food Chemistry ISTH2017 ), and collagen(4 B), Dihydrocapsaicin ) ± presented and SEM, ADP 1 μM , to in on - Murray J Adams : ), and incombination (7.5 at of are TXB Data and Fig μM . Sysmex capsaicinoid . 2014; 134: 412 . the 2 their 4 are 9 . concentration /Lusing PPP from the samesubject. mean The of CAP and DHCand a60:40 presented combination μg AggRAM B A effects

- [TXB2]% [TXB2]% ± Organic 100 105 100 105 . 1970; 34: 248 ) /mL) were addedseparately to 85 90 95 85 90 95 SEM, and 1000ianalyser (Roche as to of with (12.5 (6.25 n= μM each percent CAP - CAP capsaicin on . 1968; 442 AA 7. AA 4 . 2006; 54: 9303 + + . AA m m **p< TXB M) M) column aggregometer and 6.25 1,2 only 0 of 2 . - 001 (12.5 formation (6.25 TXB 256. (CAP), (i DHC . μM DHC . AA e AA μg + compared - + . ** 2 446. m m 0 M) concentration M) /mL) in the μM CAP + 5 dihydrocapsaicin ** . - μM 9311. capsaicinoid (3.75 (7.5 (2.5 (5 to DHC CAP ** DHC CAP AA AA m + + + + control m m M) (final m M) M) M) (Helena μM normalised μM (i ) . . e Results and . , (DHC) 0  to M