USP20 Antibody Cat. No.: 46-558

USP20 Antibody

46-558 (1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

HOST SPECIES: Goat

SPECIES REACTIVITY: Human

HOMOLOGY: Expected Species Reactivity based on : Mouse, Rat, Dog, Pig, Cow

IMMUNOGEN: The immunogen for this antibody is: C-HGEQKIEAETRAV

TESTED APPLICATIONS: ELISA, IHC, WB

Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in Human Tonsil lysates (calculated MW of 102kDa according to NP_006667.3). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In APPLICATIONS: paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat). Recommended concentration, 3 ug/ml.

September 26, 2021 1 https://www.prosci-inc.com/usp20-antibody-46-558.html Reported variants represent identical protein (NP_006667.3; NP_001103773.2; SPECIFICITY: NP_001008563.2).

POSITIVE CONTROL: 1) Cat. No. 1369 - Human Lymph Node Tissue Lysate

Properties

Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide.

CLONALITY: Polyclonal

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

CONCENTRATION: 500 ug/mL

STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing.

Additional Info

OFFICIAL SYMBOL: USP20

USP20, VDU2, specific protease 20, KIAA1003, pVHL-interacting deubiquitinating ALTERNATE NAMES: enzyme 2, ubiquitin specific peptidase 20, LSFR3A

ACCESSION NO.: NP_006667.3

PROTEIN GI NO.: 183397134

GENE ID: 10868

Background and References

1) Curcio-Morelli C, Zavacki AM, Christofollete M, Gereben B, de Freitas BC, Harney JW, Li Z, Wu G, Bianco AC. Deubiquitination of type 2 iodothyronine by von Hippel- REFERENCES: Lindau protein-interacting deubiquitinating enzymes regulates thyroid hormone activation. J Clin Invest. 2003 Jul;112(2):189-96.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 26, 2021 2 https://www.prosci-inc.com/usp20-antibody-46-558.html