USP20 Antibody Cat. No.: 46-558
USP20 Antibody
46-558 (1ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Specifications
HOST SPECIES: Goat
SPECIES REACTIVITY: Human
HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
IMMUNOGEN: The immunogen for this antibody is: C-HGEQKIEAETRAV
TESTED APPLICATIONS: ELISA, IHC, WB
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 100kDa band observed in Human Tonsil lysates (calculated MW of 102kDa according to NP_006667.3). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In APPLICATIONS: paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Data obtained from a previous batch (different goat). Recommended concentration, 3 ug/ml.
September 26, 2021 1 https://www.prosci-inc.com/usp20-antibody-46-558.html Reported variants represent identical protein (NP_006667.3; NP_001103773.2; SPECIFICITY: NP_001008563.2).
POSITIVE CONTROL: 1) Cat. No. 1369 - Human Lymph Node Tissue Lysate
Properties
Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide.
CLONALITY: Polyclonal
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION: 500 ug/mL
STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing.
Additional Info
OFFICIAL SYMBOL: USP20
USP20, VDU2, ubiquitin specific protease 20, KIAA1003, pVHL-interacting deubiquitinating ALTERNATE NAMES: enzyme 2, ubiquitin specific peptidase 20, LSFR3A
ACCESSION NO.: NP_006667.3
PROTEIN GI NO.: 183397134
GENE ID: 10868
Background and References
1) Curcio-Morelli C, Zavacki AM, Christofollete M, Gereben B, de Freitas BC, Harney JW, Li Z, Wu G, Bianco AC. Deubiquitination of type 2 iodothyronine deiodinase by von Hippel- REFERENCES: Lindau protein-interacting deubiquitinating enzymes regulates thyroid hormone activation. J Clin Invest. 2003 Jul;112(2):189-96.
ANTIBODIES FOR RESEARCH USE ONLY.
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September 26, 2021 2 https://www.prosci-inc.com/usp20-antibody-46-558.html