PYGL Rabbit Polyclonal Antibody

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PYGL Rabbit Polyclonal Antibody CAB6710 Product Information Protein Background Size: This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1, 4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from 20uL, 50uL, 100uL, 200uL inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal Observed MW: controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that 110kDa are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just Calculated MW: those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in 93kDa/97kDa moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Applications: WB IF IP Immunogen information Reactivity: Gene ID: 5836 Human, Mouse, Rat Uniprot P06737 Antibody Information Recommended dilutions: Synonyms: WB 1:500 - 1:2000 IF 1:50 - PYGL; GSD6 1:200 IP 1:50 - 1:200 Source: Rabbit Immunogen: Recombinant fusion protein containing a sequence corresponding Isotype: to amino acids 1-280 of human PYGL (NP_002854.3). IgG Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. Purification: Affinity purification Copyright © 2021 Assay Genie [email protected] www.assaygenie.com Product Images Western blot analysis of extracts of various cell lines, using PYGL antibody (CAB6710) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (CABM00020). Exposure time: 90s. Immunofluorescence analysis of MCF-7 cells using PYGL antibody (CAB6710). Blue: DAPI for nuclear staining. Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug PYGL antibody (CAB6710). Western blot was performed from the immunoprecipitate using PYGL antibody (CAB6710) at a dilition of 1:1000. Copyright © 2021 Assay Genie [email protected] www.assaygenie.com .

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Molecular Diagnosis of Glycogen Storage Disease and Disorders with Overlapping Clinical Symptoms by Massive Parallel Sequencing
© American College of Medical Genetics and Genomics ORIGINAL RESEARCH ARTICLE Molecular diagnosis of glycogen storage disease and disorders with overlapping clinical symptoms by massive parallel sequencing Ana I Vega, PhD1,2,3, Celia Medrano, BSc1,2,3, Rosa Navarrete, BSc1,2,3, Lourdes R Desviat, PhD1,2,3, Begoña Merinero, PhD1,2,3, Pilar Rodríguez-Pombo, PhD1,2,3, Isidro Vitoria, MD, PhD4, Magdalena Ugarte, PhD1,2,3, Celia Pérez-Cerdá, PhD1,2,3 and Belen Pérez, PhD1,2,3 Purpose: Glycogen storage disease (GSD) is an umbrella term for a Results: Pathogenic mutations were detected in 23 patients. group of genetic disorders that involve the abnormal metabolism of Twenty-two mutations were recognized (mostly loss-of-function glycogen; to date, 23 types of GSD have been identified. The nonspe- mutations), including 11 that were novel in GSD-associated genes. In cific clinical presentation of GSD and the lack of specific biomarkers addition, CES detected five patients with mutations in ALDOB, LIPA, mean that Sanger sequencing is now widely relied on for making a NKX2-5, CPT2, or ANO5. Although these genes are not involved in diagnosis. However, this gene-by-gene sequencing technique is both GSD, they are associated with overlapping phenotypic characteristics laborious and costly, which is a consequence of the number of genes such as hepatic, muscular, and cardiac dysfunction. to be sequenced and the large size of some genes. Conclusions: These results show that next-generation sequenc- ing, in combination with the detection of biochemical and clinical Methods: This work reports the use of massive parallel sequencing hallmarks, provides an accurate, high-throughput means of making to diagnose patients at our laboratory in Spain using either a cus- genetic diagnoses of GSD and related diseases.
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