Keynote Address

M. Sanjappa Mahatma Gandhi Botanical Garden University of Agricultural Sciences GKVK, Bengaluru 560064 Email: [email protected]

Today, no one science can be studied in isolation. Trans-disciplinary Research is defined as research efforts conducted by investigators from different disciplines working jointly to create new conceptual, theoretical, methodological, and translational innovations that integrate and move beyond discipline-specific approaches to address common problem. Trans-disciplinary approaches have become necessary. Some of the revolutionary discoveries in Biological Science like the double helix coil of life (i.e. DNA) are due to the developments in Chemical and Physical Sciences. Plants and animals are one of Earth’s greatest resources. They are sources of food, medicines and materials with vast economic and cultural importance. The plants stabilize ecosystems and form the habitats that sustain the planet’s animal and microbial life. Both plants and animals are also threatened by climate change, the environmental factors and human interaction. Each species has its own unique characteristics and in a world that is increasingly moving towards a global bioeconomy, each organism is a potential source of materials that are of benefit to mankind.

Over the centuries new technologies were/and are being developed to sustainably use both living and nonliving resources to provide goods and services. Of over estimated about 8 million living organisms on earth less than 2 million species have been documented since the beginning of scientific study of living organisms. Continuous efforts are made to understanding their relationships rather than making concerted efforts to complete their documentation and conservation to sustainably utilise them. Simultaneously new technologies like biotechnology are developed to meet ever growing needs of exploding human populations, to maintain balance of reducing populations of other living things with which we share this planet and the ever degrading habitats and ecosystems essential for continued existence of life on earth. Briefly the emerging trends in biodiversity and biotechnology are presented here.

INDEX

Sr. Oral / Page Theme Author No. Poster No. Theme-A Agriculture crop sciences and forestry sciences Utilization of Organic Manures with Inorganic Fertilizers and effects on Growth Kale V. R. and Salve U. S. 1. PP 03 &Yield Components of Soybean (Glycine Max L. Merill.) Geochemistry of groundwater in an Salve P. L., Gaikwad S.G., 2. agricultural area of , , OP 04 Aher K.R, Sulochana Ramsing Rathod, Control of seed-borne Alternaria species 3. Surekha Anil Saraf, Suchita OP 05 using extraction of medicinal plant P. Bharambe Impact of Chemical Mutagens on Cluster V. R. 4. Bean (Cyamopsis tetragonoloba (L.) Mhaske and M. S. Wadikar OP 06 TAUB.) Effect of N – Fertilizers on silage Smita Basole 5. PP 07 fermentation Sarwade B. P. R.P. Patil Studies on influence of leaf litter composts 6. &M. A. Kare OP 08 on yield and nutrient of beet root

Antimicrobial Activity of Some Indian R.G. Biradar 7. PP 09 Medicinal Plants against Seed Mydcoflora “Back Cross Programme on Sesamum Ms. Srushti S. Kale & Mr. J. 8. OP 10 Indicum” D. Salunke. Evaluation of Organic Compost for Physico- G.M. Birajdar, S.P.Surve and Chemical Analyses and impact on Soybean O/P 6. U.N.Bhale 11 and Wheat Vigour Growth under farmers’

conditions Mrinal P. Joshi; Kishor A. Effect Of Mutagenesis On Seed Matsagar; Indraneel V. O/P 7. Germination In Two Different Varieties Of 12 Jiwane; Seema V. Dongre Cicer Arietinum L. and Arvind S. Dhabe “Nutritional Status Assessment, Effects and Bharati S. Tapase OP 8. Evaluation of Water and Waste Water 13

on Crops” Oligomers as Natural Growth Promoting Saoji D.D.,Kottayi M.C. & 9. OP 14 Agents Chaudhari A.D Theme B and C B- Aquatic botany Aquaculture and fishery Science C-Wildlife Conservation and Forensic Sciences Toxicity impact of Cestrum nocturnum on Jawale C.S. and Singh-Gupta 10. respiratory metabolism of Fish Clarias PP 16 S. batrachus Histhopathological Study of Senga sp. Yogesh Reddy, Hariom Infected in Freshwater Fish Mastacembelus 11. Ingale and Aniket Karpe OP 17 Armatus Effect of Ekalux (25 % Ec) on Histopathological Changes in Liver of Nilesh Pakhare 12. OP 18 Freshwater Fish Channa Gachua (Hamilton, 1822) 13. Diversity and Conservation of Ceropegia L. Tanveer A. Khan PP 19 INDEX

from Satpuda range of district (M.S.) Sangeeta ahuja and narayan Studies in Pollution Controlling Plant 14. pandhure PP 20 Species of

Uses of some Medicinal plants For Wound Manish V. Bankar 15. Healing In District M.S.(India) OP 19

Study on Biodiversity of Higher species and Choudhari S.S. 16. its depletion causes in Ambachandi Bala OP 22 Ghat Ranges Dharur Dist. Beed Taxonomic Studies on Weed Diversity of R.D. Gore & V.S. Chatage 17. OP 23 Region of Maharashtra State Awareness regarding the conservation of Dr.Pratap V.Deshmukh 18. Biodiversity illiterate peoples: Aundha OP 24 Dr.V.S.Maske region. Diversity, Conservation Status and Dr. Ghadsing P.G 19. PP 25 Medicinal Plants of the Euphorbia Dr.I.B.Salunkhe Biodiversity and limnological Profile of Ahirrao S.D. 20. Masoli reservoir from District of O/P 26 Maharshtra Current Field Status, Diversity and Distribution of AcanthephippiumBl. Rajendra Yonzone 21. OP 27 (Orchidaceae) in Darjeeling Himalaya of , India Biodiversity of Phytoplanktons Of Vidya D Gulbhile 22 , Parli- Vaijnath Dist, PP 28 Beed Maharashtra, India 23. Protection of Plant Varieties in India Bandewar S.T and OP 29 T.D.Kamble Theme – D Ecology and Eco -toxicology Degradation of Cellulose Containing NAYAB ANSARI 24. Effluent OP 31 By Trichoderma Dr. Dhond G.M., Jadhav Physicochemical Water Analysis of 25. R.L. OP 32 Bindusara river in ,(M.S) “Effect of air pollution on chlorophyll Dr. Rajmane S.D. O/P 26. content of Terminalia procera and 33 Magnifera indica” Physico-Chemical analysis of Soil samples in V.V.Naiknaware O/P 27. 34 Beed District, Maharashtra. Theme - E Economic botany Zoology Microbiology and biotechnology Redescribed Nematode species of Swati Jadhav, Atul Humbe Procamallanus Arnai (Lakshmi I.R., 2010) 28. in Mastacebelus Armatus (Lacepede, 1800) and Nitin Padwal OP 36 in Sina Kolegoan Dam, (Ms) The role of enriched supplementary feed in 29. Shaikh H.M. Patel Y.E. PP 37 relation to growth of Labeo rohita. INDEX

Optimization of Exopolysaccharide Chudiwal A.B . and production by marine haloalkalotolerant 30. Dharmadhikari .S.M. OP 38 Psudomonas aeroginosa using response

surface technique. Analysis of some domestic species and their S. T. Naphade, P. S. Patil products in Ajanta hill ranges with 31. and S. R. Naphade OP 39 reference to traditional medicine for health care in rural population. Coccidiosis in Backyard Poultry Chicken Bhimrao N. Jadhav 32. (Country Chicken) in Vaijapur Tehsil of OP 40

Aurangabad District (M.S.) A New Species of the Genus Tylocephalum Ashok Mote, S. R. Gaur V 33. Linton (1890) From Intestine of Marine D Suryawanshi OP 41 Fish Eregoodoo Monitoring of Blood Group ABO & Rh(Rhesus Blood Groups)Distribution of M.R. Yeotkar 34. OP 42 One Year Survey in Anjangaon Surji Region Dist- -Maharashtra Study of Garlic (Allium sativum) effect on Deshmukh P.S. 48. control measures of land snail, PP 43

Macrochlamys petrosa. Rajni S. Amrawkar and Study of fresh water fish diversity of 49. Phulwade Durgesh N. PP 44 Phulmabri Dam, Aurangabad. (M.S).

A new species Lytocestus parturii of the genus Lytocestus (Cestoda: Lytocystiodae, R. G. Pradhan and R. M. 35. Hunter, 1927) from the fresh water fish OP 45 Dhere Clarius batrachus at. Partur Dist. Jalna. (M.S.), India. Application of immobilized beads of Ps. Kulkarni R. A. and Gupta S. aeruginosa 4442 and role of various elutants 36. G. OP 46 on Cr (VI) recovery from metal containing

solution Optimization of Metallo-Collagenase Savita Kate Production by Arthrobacter creatinolyticus 37. Archana Pethe OP 47 KP015744 zzx28I solated From

Deteriorated Leather Sample Planktonic Biodiversity and its importance Sitaram B. Ingole, Prashant 38. role in fish production on Dam Sanghai OP 48 reservoir, Maharashtra State. India. Avifaunal Diversity Found In and Around Kakde Vandana R. 39. Dnyanganga Abhayaranya of Buldana PP 49

District (M.S.)- A Report INDEX

Protein Estimation in the Body of Dr. Nilima M. Kankale, Dr. 40. Tetragonocephalum Pulensis From A Varsha M. Jaisingpure OP 50 Marine Water Fish Trygonsephen Sudheer S. Senigarpu M.Sc, Effect Of Sodium Fluoride On Vas deferens 41. Dr.V.T.Dhurvey OP 51 of Male Albino Rats (Rattus norvegicus)

Biochemical Studies Of Cestode Parasite A. M. Budrukkar 42. Raillietina Fuhrmann of Gallus Gallus OP 52

Domesticusfrom Georai Prevalence of Bacterial Pathogens in Sayed Rizwan A and Bagmar 43. OP 53 Vegetables Commonly used as Salads Chitra Avian Polyspecific Clutch size as Chandrakant V. SIRSAT 44. Bioindicators of Ecological Condition of the OP 54

area Study on hygienic condition in Fish Market D. R. Deshmukh and 45. OP 55 of Dist.Aurangabad Maharastra. L.B.Dama “Nematicidal activity of some common plant Shaikh Unaiza Nazneen, 46. extract on population density of soil J.D.Shaikh and Shaikh OP 56 Nematode.” Tarranum Taneer Isolation of Lactic Acid Bacteria Manisha Nitin Maind 47. [PROBITICS] from homemade fermented PP 57

food sample for Yoghurt formation. Estimation of Protein Content in Fish M. S. Kadam Muscles from the local Fish market, OP 59

city Comparative Study of Wings of Butterfly H. P. Nandurkar 50. Graphium Agamemnon and moth Thyas OP 60

Coronataby Scanning Electron Microscopy S.B. Patharde and Kinetics of Renal Alkaline Phosphatase of 51. A.M.Chilke OP 61 catfish, Clarias gariepinus

“Studies on biology of fruit piercing moth Shendge E.S, Kayande H.G, 52. Othreismaterna (Noctuoidea: Lepidoptera) Chavan R.J. OP 62 from Marathwada (M.S.) India” A Comparative Study of Azotobacter spp. M.T. Kuchekar and B.T. 53. from various Localities of Beed District Pawar OP 63 (M.S.) Sayad Jalil Hussain, Living Habitat of Freshwater Fish Rasbora 54. Mohammad Ilyas, Shaikh OP 64 daniconus in Bindusara Basin, Beed. Feroz. Deepti D. Dhere1 and S. M. 55. Carotenoid: As a bioactive compound OP 65 Dharmadhikari2 Checklist of Wasps, Bees and Bumble Bees Gurule Sachin Arjun, 56. (Insecta: Hymenoptera) from K.T.H.M. Gangurde Jyoti Haribhau and OP 66 College Campus, . Jadhav Tushar Ananda Comparative Study of Wings of Butterfly H. P. Nandurkar 57. Graphium Agamemnon and moth Thyas OP 67

Coronataby Scanning Electron Microscopy Patidar Kavisha K., Dr.S. A. Enrichment, isolation and screening of 58. Peshwe PP 68 potential pectinase producing organism

INDEX

Mr. Sopan A. Evaluation of Antibacterial Activity of Leaf Extract Shinde Dr. Vanita 59. of Jasminum Officinale Against Oral Pathogens in OP 69 U. Pochhi Ulcer Treatment

“Preliminary investigation of Protozoan ciliates of Sanghai P.K. 60. Family Isotrichidae from the rumen of domestic O/P 70

goat” Gadakh PV and Biosurfactant: A New Alternative to Chemical 61. Pawar AL OP 71 Surfactant for Bioremediation

Atish R. Mehetre, Synthesis and Antimicrobial Study of Rare Earth Sandeep R. 61a Complexes of Schiff Base Derived from 4-Amino Deshmukh and OP 72 Benzoic Acid Vijay N. Bhosale Changes in the glycogen content of freshwater bivalve Indonaia caeruleus (Prashad, 1918) with Thorat S. K, 62. injections of cerebral ganglionic extract and Vedpathak A.N. OP 73 equivalent commercial hormones (progesterone and estradiol) during winter season. Comparison of Phospholipase A2 from Honeybee Vijayshree Hemke 63. OP 74 Venom by In Silico Method Sakale S. S. & Development and Characterization of Bacterial 64. Chitanand M. P OP 75 Consortium for Bioremoval of Cr (VI).

Morphological Studies of Chilodonella cyprini T.T.Shaikh 65. PP 77 (Moroff 1902) from freshwater fishes K. D. Thete, L. V. Analysis of Physico Chemical Parameter of Soil Shinde, S. B. O/P 66. Samples and Nematode Diversity of Seasonal Crops 78 Bhadange and R. B. from Sakur Region, Tal. Sangamner (Ms) India. Antre Study the Effect of pH and Temperature on Protease Syed Nisar Ahmed, O/P 67. Production by Psychrotrophic Pseudomonas Species Mohammad Shakir 79 Isolated from Dairies of Aurangabad. “Ultrastructure of The Skin of The Tree Frog” I. R. Ustad 68. OP 80 Polypedates Leucomystax S.R. Sule, “Studies on Diversity of Mosquito Larvae in R.J.Chavan, 69. OP 81 Aurangabad City, Maharashtra, India’’ L.V.Shinde, H.G Kayande. Sushama Paikade, Prevalence of Ectoparasides Infestation on Milch Ramrao Chavan, 70. Cattels From Aurangabad District, Aurangabad, (M. OP 82 Hanumant Kayande S) India.

Analysis of Enzyme Activity during Vishal Bedre and O/P 71. Vermicomposting of Organic Wastes (corn cob and Balraj Khobragade 83 cauliflower) employing the earthworm Eudrilus eugeniae Antibiotic Resistance Pattern among Fecal Indicator R. N. Jadhav O/P 72. 84 Bacteria in Hospital Waste Water INDEX

Planktonic Biodiversity and its importance role in Sitaram B. Ingole, 73. fish production on reservoir, Prashant Sanghai OP 85 Maharashtra State. India. Efficacy of lawsone on survival, growth and Sujata Magdum and 74. metamorphosis in fourth instars nymphs of Boisea Rajashri Shinde OP 86 coimbatorensis Gurule Sachin, Preliminary studies on composition and diversity of Supriya Vivek and 75. beetles (Insecta : Coleoptera) in , OP 87 Pawar Kajal Maharashtra, India.

Temperature Influence on the Rate of Development J.S. Dahegaonkar 76. of Life Stages of Gram Pod Borer, Helicoverpa And A.S. Mohite PP 88 Armigera (Hübner) (Lepidoptera: Noctuidae) S. G. Kamble, R. D. Biochemical Variationin Fresh Water Bivalve Sonwane and G. D. 77. PP 89 Lamellidens Marginalis Suryawanshi

Diversity of Some Lepidopterian Fauna of Chikhli M.T. Nikam 78. O/P 90 Region Dist. Buldana Maharashtra State. Role of Cerebral Ganglia in Regulation of Oxygen Consumption of Freshwater Bivalve Mollusc, Deepak M Gaikwad 79. OP 91 Lamellidens Marginalis from At Kaigaon During Monsoon Season In Vitro Effect of household pesticides and heavy metals on the growth of Azotobacter chroococcum Dr.Mrs. Jadhav P.N. 80. OP 92 and other microbes isolated from sewage irrigated fields. “Effects of Estradiol hormone on the heart beat rate 81. and oxygen consumption in freshwater Bivalve Kayande H.G OP 93 Lamellidens marginalis (Lamark)” On A New Species of Genus Tylocephalum (Linton, Vasant Dongare 82. 1890)(Cestoda: Lecanicephalidea) From Dasyatis PP 94

Walga from Dist. Raigad (M.S) India Chandrakant V. Avian Polyspecific Clutch Size As Bioindicators of 83. SIRSAT PP 95 Ecological Condition of The Area

R.P.Patil, Study of Antibacterial and Antioxidant activity of L.H.Kamble and 84. PP 96 Arisaema tortuosum Schott V.C.Patil

Nutrient Dependent Microfaunal Diversity At Masoli S. M. Yeole 85. OP 97 Reservoir, Parbhani (M.S.), India Survey of helminth parasites in freshwater fishes Ravi Solunke 86. 98 from Marathwada region, MS, India Study of alimentary tract of Channa punctatus from Ajit K. Gedam 87. PP 99 Kaigaon Toka, Aurangabad. Afreen Rehman “Study on zooplankton diversity from Rui 88. Deepak Gaikwad OP 100 Project,Osmanabad(MS) India” and Sunil Sherkhane INDEX

Redescription of the plant nematode Deepak Gaikwad 89. Helicotylenchus vulgaris Yuen, 1964, PP 101

(Nematoda: Hoplolaimidae) Yogesh Reddy, Anilkumar Application of Correlation Techniques of Pardeshi, Nilesh Pakhare, 90. Image Processing Database for Snakes OP 102 Utkarsh Jadhav, Shubham Identification Malekar Determination of the prevalence of Babesia V.S. Ingle, V.R. More. 91. DNA in the blood samples of Goat from OP 103

Aurangabad by PCR. Nagawanshi M N Khedkar 92. Labelling of Spider with DNA barcode” OP 104 G D Nutrient Dependent Microfaunal Diversity At S. M. Yeole 93. OP 105 Masoli Reservoir, Parbhani (M.S.), India Avhad Sunil. B “Hemiptera: Distribution, Occurrence, Type of 94. Hiware Chandrashekhar. J OP 106 Damage And Symptoms on Mulberry (Morus

Alba L.)” In Vitro Effect of household pesticides and heavy metals on the growth of Azotobacter Dr.Mrs. Jadhav P.N. 95. O/P 107 chroococcum and other microbes isolated from sewage irrigated fields. “Population Fluctuation of Helicotylenchus Avhad Sunil. B 96. Steiner, 1945 In Relation to Soil Abiotic Factor Hiware Chandrashekhar. J OP 108 In Mulberry Field” Leidynema appendiculata (Leidy,1850) Chitwood,1932. (Oxyuroidea: Gavali S.V. Dr. Ashok 97. Thelastomatidae) found infecting Cockroaches Mote,Dr. S .N. Borde PP 109 Periplaneta americana (Dictyoptera:Blattidae ) from Aurangabad city,(MS), India. Morphological Studies of Chilodonella 98 Cyprini(Moroff 1902) from T.T. Shaikh OP 110 freshwater fishes On A New Species of Genus Davainea 99 (Blanchard, 1891) from Gallus Domesticus At Dr. Ishrat Vasi Shaikh OP 111 Gangapur, Aurangabad, M.S., India. On A New Species of Genus Tetragonocephalum (Shipley & Hornell, Vasant Dongare and 100 OP 112 1905)(Cestoda: Lecanicephalidea) From Yogesh Reddy Dasyatis Walga from Dist. Raigad (M.S) India 101 Image of flower 113 Analysis Of Hair Sample Of Some Wild Animals In Relation With Diameter And Yogesh Reddy and A.B. 102 OP 114 Medullary Patterns Using Microscopic Pardeshi Technique INDEX

Isolation, Characterization And Insecticidal Kalane V. G., Jadhav S. 103 Potential Of Bacillus Subtilis And Alloiococcus M., Jadhav P. N. and OP 115 Otitis Against Helicoverpa Armigera Hubner. Pardeshi A. B. Influence of Phytoecdysteroid Isolated From Barge S. B., Vetal D. S., 104 Dalbergia Sissoo on Economic Parameters of Nagare G. J., and Pardeshi PP 116 Silkworm, Bombyx Mori L. A. B Histopathological Changes in Labeo rohita 105 Exposed to Mercury with Banana Peel as S. A Shaikh OP 117 Bioadsorbent Biopesticidal Activityof Cassia Tora Against Red Gadewad M. G., Jadhav S. 106 OP 118 Cotton Bug, Dysdercus Cingulatus Fab. M. and Pardeshi A. B., Natural saponin in Sapindus emarginatus controls Bajad P. N., Pardeshi A. 107 the protein loss in Phaseolus aconitifolius grains PP 119 B. and Pagore V. P. caused by Callosobruchus chinensis To Study the Endoparasites In Freshwater Laxmi Indani and S. A. 108 Fishesmastacembelus Armatus, Channa Gachuain PP 120 Shaikh Maharashtra, India. Revision of the Genus Xiphocephalus and Description of Xiphocephalus arnatakaensis Kanse V. S., Nagawanshi (Apicomlexa: Eugregarinida : Stylocephalidae) 109 M.N , Pardeshi A.B. and OP 121 From Tenebrio molitor (Coleoptera: Chondekar R.P. Tenebrionidae), In , Marathwada, M.S. India Prevalence and seasonal rate of infestation of Jyoti Shirsat and V.R 110 helminth parasite in fresh water fish in OP 122 More Aurangabad region (MS) India Preliminary studies on composition and diversity Gurule Sachin, Supriya 111 of beetles (Insecta :Coleoptera) in Nashik district, OP 123 Vivek and Pawar Kajal Maharashtra, India. Theme F Ethnobotany, Biopesticide and bioactive compounds Ethno veterinary medicine from , Chavan R.T. 112 OP 125 Maharashtra India Prof. Bhagwan M. Significance of indigenous medicinal plant and 113 Waghmare OP 126 their utilisation

Pharmacognostic studies of Sphagneticola Sangeeta S. Sutar 114 PP 127 trilobata[L.] Pruski. Asteraceae Survey of Aquatic and Submerged Aquatic Kondekar Chaitali and Dr. 115 Medicinal plants from Rahati area of Parbhani. Syed Sabiha PP 128 (MS) Sunita Bhosle, Smita Review: Ethanobotanical properties of Turmeric 116 Basole and Savita Sukte OP 129 in Maharashtra

Efficient Synthesis of 2, 4, 5- Triarylimidazoles: A Arshia Parveen 117 PP 130 Bioactive Molecule Effect Of Parthenium Hysterophorus Linn. OnSeed S. Kshirsagar, V. Bhise, 118 Germination, And Studies The Antimicrobial D. Gaikwad, P. Solunke, OP 131 Activities. R. Wakde*, Dr K. Prashar Medicinal uses of Plants by Indigenous people of Vijigiri Dinesh G. 119 Mahu Taluka, , Maharashtra, OP 132

India. INDEX

An efficient L-proline promoted synthesis and A.V. Nakhate, S. V. 120 antimicrobial study of 2,5-disubstituted-1,3,4- Shinde2 OP 133 oxadiazoles Sunil U. Tekale, Satish U. Synthesis of 1h-Indazoles Using Lemon Peel Deshmukh, Ajit K. Dhas 121 Powder As A Natural, Green and Efficient OP 134 and Rajendra P. Pawar Catalyst Under Ultrasound Irradiation

Base catalysed one pot green synthesis of Vijay P. Pagore 122 OP 135 Tetrahydrobenzo[b]pyran R. D. Ingle, C. S. Patil, S. Green Synthesis of Pyran Derivatives Using R. Bembalkar, M. V. 123 Lemon Peel Powder as a Natural Catalyst and OP 136 Katariya and R. P. Pawar their Antimicrobial Activity

Ethanobotanical Significance of some Medicinal Shweta Kamble, Shamal 124 plants from family Cucurbitaceae and Mali, Dnyaneshwar Mane OP 137 Asclepiadaceae and B.M. Waghmare BioprospectingPentapteras of Genus: Terminalia Salve, A. P. & Dhabe, A. O/P 125 L. of Maharashtra state, 138 S. India for Antioxidant activity. Study of Ethenobotanical Plant's Used on O/P 126 Calculus (Kidney And Galbladder Stone Ladda R.G. 139 Problems) Theme – G & H G- Ecology and Eco-toxicology H-Microbiology and Aerobiology Dr.Sanjay A. Kamble, Dr. Aerobiology in the transmission of Infectious 127 M.S. Kharate OP 141 Diseases through Droplet air Infection.

Determination of Potential Allergenic Fungal R.M. Kadam 128 flora and its Clinical Reflection in Urban and PP 142 Rural Areas of District of Maharashtra V.S. Nagpurne 129 Indoor Air Quality and Health Impacts OP 143

Antibacterial Acitivity of Some Fruit Extract Sanghamitra V. Arak and 130 Against Xanthomonas Tagetes Rangaswami and R.J. Sawant PP 144 Sanne Gowda The Role of Epicoccum as a biopollutant S. W. Bhivgade, S.N. 131 PP 145 regarding asthma Sangekar and U.S. Salve G.M. Pathare and 132 Aeromycoflora over Sunflower Field OP 146 P.S.Anarse Theme I Physiology and Biochemistry The Quantitative Measurement of Leaf Rajput R. D., Solanke S. 133 Chlorophylls and Carotenoids from some N.and Patil R. P OP 148 leguminous fodder crops by Spectrophotometry Changes in Coriander (Coriandrum Sativum L.) 134 leaf Juice During Storage With Reference to the U. S. Salve OP 149 Biochemicals R. U. Bhagyawant and Effect Of Electricity on Seed Germination of 135 Mukundraj B. Patil OP 150 Certain Crop Plants

Prashant Patankar and Biochemical Studies in Hybanthus enneaspermus 136 Sanjay R. Biradar OP 151 (L.) F. Muell. Medicinally Important Plant.

137 Effect of Chemical Pre-Treatments on Seed Rajesh Shrirangrao OP 152 INDEX

Germination and Seedling Growth of Syzygium Gaikwad Cumuni (L.) Skeels. Shirsat T.T, Jondhale 138 Ultrasound Induced Germination of Seeds PP 153 M.B., Kulkarni S.G Efficiency of Phytohornes on Vigour Index of Lata Dattu Done and 139 OP 154 Vigna radiata (L.) Wilczek Var. Avinash Ade Preparations of Vermin Wash Its Effects on Plant D. Rajesh and Ahmed 140 Growth And Devlopment of Red Spinach PP 155 Abdul Haleem Khan (Amaranthus Dubius) 141 Image of Flower 156 142 Image of Flower 157 Theme J Phyto chemistry Phytogeography and Bio- physics Antibacterial, Antioxidant And Phytochemical Vaishali Dange and 143 PP 159 Analysis of Ripe and Unripe Banana Peel 1Anruni Mohanty Detailed quantification of base metals and precious metals of scrap PCB of mobile phones by Sonawane P.M and Gupta 144 O/P 160 FE SEM/EDAX and Inductively Coupled Plasma- S.G. Optical Emission Spectroscopy method. Phytochemical profile of Curcuma inodora Blatt. Anand S. Jadhao and Anil 145 OP 161 Rhizome Extract S. Bhuktar Seasonal Variations Of Phytochemical Values Of 146 V. B. Kadam 162 Aegle Marmelos ‘HPTLC’ an important tool for quantification of 147 Sutare M S, PP 163 herbal product: A case study Histochemical Investigation of Madhuca indica 148 S.S.Tambe, V.B.Kadam OP 164 Gmel Evaluation of Antibacterial Activity of Leaf Sopan A. Shinde and 149 Extract of Jasminum Officinale Against Oral OP 165 Vanita U. Pochhi Pathogens in Ulcer Treatment A Study of Phyto-Alkaloid and their therapeutic 150 uses in treating neurological problems with Dr. P. Latha OP 166 certain Homeopathic Medicines Quantitative investigation of phytoconstituents 151 present in Albizia julibrissin Durazz.methanolic Wankhade, M.S. OP 167 leaf and bark extract. Qualitative analysis and TLC Screening of various Sarika V. Lokhande and 152 Stem extract of medicinally important plant OP 168 Sanjay R. Biradar Cissampelos pareira Linn. Pharmacognostic and preliminary phytochemical Shaikh M. D., Dhotre R. 153 OP 169 evaluation of the bark of dalbergia sissoo roxb. S.,R,P.Patil & M. A. Kare Ugandhar., Antimicrobial Potential And Phytochemical Venkateshwarlu., Odelu. , 154 Screening of Leaves And Fruits of Solanum OP 170 Rajendra prasad. and Thorvum (Swartz). A Medicinally Important Plant Anitha devi. S. M. Sangle, S. V. 155 Phytochemical studies of Ipomoea clarkei Hook. f. OP 171 Dongre and A. S. Dhabe Phytochemical Profile of Curcuma Inodora Blatt. Anand S. Jadhao1 and 156 OP 172 Rhizome Extract Anil S. Bhuktar Phytochemical analysis and Antibacterial activity Madhuri M. Deshmukh 157 of Dolichandrone falcate (Seem), against selected OP 173 and Navnath G. Kashid Human Pathogens. Evaluation of TLC and GCMS activity of Madhuri M. Deshmukh 158 OP 174 Dolichandrone falcata (Seem). and Navnath G. Kashid INDEX

Pharmacognostic studies on leaf drug Butea Rupali Biradar and Vikas 159 OP 175 monosperma (Lamk.) Taub. Gambhire Phytochemical Analysis of Gulwel Tinospora 160 Hirve B.J. 176 cordifolia (Wild) Miers. Leaf. Vidya N.Mhaske, Namrata A.Gadalkar, Phytochemical analysis of Bolusanthus speciosus 161 Rohini N.Karjule, PP 177 (Bolus) Harms. Supriya R.Gawade and Sagar S.Bawake Biological mediated silver nanoparticles and Ramteke N. M., Salve 162 investigation of antibacterial, P.K., Vinchurkar A.S., PP 178 antioxidation potential with qualitative profiling Kulkarni S.G. Shirsat T.T, Jondhale 163 Ultrasound Induced Germination of Seeds PP 179 M.B., Kulkarni S.G Phytochemical and Proximate Determination Kolhe P.H, Ashok Gawate 164 From OP 180 and Patil, R. P. Leaf Of Cajanus Cajan (L.) Millsp. Phytochemical and Anatomical Studies in S. N. Sangekar and V. D. 165 OP 181 Amaranthus blitum subsp. oleraceus (L.) Costea Devarkar Theme K & L. Plant Pathology, Phycology and Cryptogamic Botany Effect of Phormidium mucosum Extracts on 166 Growth and Development of Certain Legume Yadav S.G. OP 183 Crop Plants Isolation and Identification of endophytic fungi O/P 167 Deshmukh P.D. 184 from chilli (Capsicum annuum L .)plant. Studies on Antagonistic Activities Of Endophytic O/P 168 Fungi Against Soil Borne Shinde S.Y 185 Pathogens Associated From Leguminous Plants. Effect of antibiotics on Protease and Lipase Kesare U.T. And Ashok O/P 169 186 Production In Seed -Borne Fungi of Soybean Chavan Enzymatic Activity Of Fungi Associated on Fruit V. S. Chatage and U. N. 170 OP 187 Rot Of Coccinia Indica (Wight And Arn). Bhale Study of Biddulphiaceae member of Diatoms 171 along the coast of Gholvad and Tarapur, Gogari Pankaj K OP 188 Maharashtra. Present Status of Bacterial Blight on Pomegranate B. D. Pandit and N. A. 172 OP 189 From District of Maharashtra (India) Ghanawat Impact of Aeroallergens: on Human Health 173 Kalpana P Ghoshal. OP 190 Hazards. Sensitivity of Diffrent Fungicides Against Dry Rot 174 Wakle G.L. OP 191 of Potato Antimicrobial activity of crude extracts of Rahul K. Dhabale, 175 endophytic fungi isolated from Ventilago Bhagwan M. Waghmare OP 192 denticulata and Suvarna T. Kadam Study of some diatoms from shivana Takali dam 176 Anil A. Kshirsagar OP 193 of Kannad District Aurangabad. (Maharashtra) 177 Image of flower 194 Antifungal activity of Vvitex Negudo Linn and 178 Wakle G.L. PP 195 Buteamonosparm aginest alternaria hibiscicum Fungicidal Efficacy on Fusarium Oxysporum 179 Ingale S.T. and Patale S.S. OP 196 Causing Root Wilt of Chickpea 180 Studies on Phytoplankton Diversity of Godavari Ishwar.B.Ghorade1 and OP 197 INDEX

River water Kirti S. Niralwad Additions of Zygnemataceae To the Marathwada Yadav S.G. and Sabale 181 O/P 198 Region of Maharashtra C.G. Cyanobacterial Diversity of Beverage indutries Talekar S.M. and Baglane O/P 182 199 waste water A.D. Fungal Endophytes Associated with the Indian Ashfaque M. Khan, Jawed 183 laburnum (Cassia fistula L.) Shaikh and Harshdeep B. OP 200 Sartape Use of Some Plant Extracts as Safe Fungicides Gagre Patil V. A. & 184 OP 201 against Selected Phytopathogenic Fungi Vanmare D. J. Isolation and Screening of Soil Fungi in Different Patil T.V. and Pangrikar 185 OP 202 Culture Media P.P. Some species of genus Selaginella p. beauv. from Neel R. S. and Bhuktar A. 186 OP 203 Maharashtra S. J. J. Kshirsagar, G.L. Fungal Spores of Soybean field, Rajuri (N), Dist. 187 Pachkore, A.N. OP 204 Beed. Dharasurkar Yogita S. Patil , H.A. 188 Algal flora of water, Nasik (M.S) OP 205 Thakur , B.N. Zaware , Invitro Antifungal Efficacy Of Spondias pinnata Aarti M Patil, Sadat. M. 189 Kurz. Against Colletotrichum falcatum Went. Quazi and Seema. M. OP 206 Causing Red Rot Disease In Sugarcane Sathe Nilam Tupe and Suchita 190 Fungal Airspora over Cajanus cajan L. OP 207 Rajurkar Biochemical value changes in infected Banana Sadhana Salve and 191 OP 208 fruit from (MS). Suchita Rajurkar Isolation and identification of endophytic fungi OP 192 R. B. Allapure 209 from Solanum nigrum Assessment of Morphological Characteristics, Kalpana Kulkarni and OP 193 Growth and Sporulation of the field isolate 210 Swati Peshwe Magnaporthe oryzae ch1. Diversity of Wood rotting Aphyllophorales from OP 194 F. H. Chouse, V. P. Mali 211 Bhoom tahsil (Osmanabad district) Maharashtra Mycoflora Isolation Form White Pumpkin Anil U. Kulkarni & OP 195 212 (Cucurbita pepo L.) Shrimant A. Survase In Vitro Dominance of Bacillus amyloliquefaciens RRR15 for R. R. Rakh L. S. Raut and OP 196 213 Microbiological Control of Sclerotium rolfsii S. M. Dalvi Sacc., A Stem Rot Pathogen of Groundnut Investigation of Phosphate Solubilizing Bacteria Sanjay M. Dalvi, V. N. OP 197 (PSB) from Rhizospheric 214 Kadam and R. R. Rakh Niches of Healthy Plants Algal flora of maize field soil in Milind J. Jadhav and OP 198 215 District of Maharashtra. Swati G. Wagh Influence of post-harvest fungi on biochemical Suvarna T. Kadam, changes of selected edible OP 199 Bhagwan M. Waghmare 216 nut seeds stored in commercial markets in and Rahul K. Dhabale Marathwada Prevalence of Bacterial Pathogens in Vegetables Sayed Rizwan A and OP 200 217 Commonly Used as Salads Bagmar Chitra Rafiullah M. Khan1 And OP 201 Chlorophycean algal flora of Lonar Crater Lake 218 Milind J. Jadhav INDEX

Natural Saponin in Sapindus Emarginatus Controls the Protein Loss In Phaseolus Bajad P. N., Pardeshi A. OP 202 219 Aconitifolius Grains Caused by Callosobruchus B. and Pagore V. P. Chinensis Qualitative study of Zooplankton in relation to Rahul Mahamuni and OP 203 anthropogenecity at Nathsagar Wetland of 220 Kshama Khobragade Paithan Taluka of Aurangabad District Inhibitory Activity of Syzygium cumini L. Leaf Satpute S.B. and Vanmare OP 204 221 Extracts on Fungi D.J. Effect of different environmental conditions on the antimicrobial activity of Pseudomonas OP 205 Bharade Sunita S. 222 fluorescens against Alternaria alternata (fr.) Keissler causing fruit rot of pomegranate Study of Coscinodiscus Phytoplankton diversity of OP 206 Pankaj K. Gogari 223 Tarapur and Gholvad coast of Maharashtra Efficacy of fungicides against Stemphylim blight OP 207 A.R Kolte and S.S Patale 224 and Purple blotch of Garlic (Allium sativum L.) Phytoplankton Seasonal Abundance in Relation to O/P 208 Physico-Chemical Features in Mahapoli Lake, Rafatunisa Nahri 225 , Maharashtra Biosynthesis and characterizations of silver Taur S. A. and Vanmare O/P 209 226 nanoparticles from Aspergillus D.J. Effect of Phormidium mucosum Extracts on O/P 210 Growth and Development of Certain Legume Yadav S.G. 227 Crop Plants Studies on fungal foliar diseases of Adulsa O/P 211 B.M.Kareppa, 228 (Adhatoda zeylanica Medic) Hitherto on unreported macrofungi from Vishal Wakode and O/P 212 229 Aurangabad (M.S.) India Sangeeta Ahuja Balwan W. Kamble, Study the effect of substrate and non substrate Shrimant A. Survase, Anil O/P 213 medium on the pectinase activity of fungi from 230 U. Kulkarni and Ashok M. vegetables waste Chavan Isolation and screening of some Aphyllophorales Rangnath G. Kanthale and 214 O/P 231 for production of Laccase Aparna S. Taware Fungal endophytes : potential source of indole Narwade P. S. and Taware OP 215 232 acetic acid A. S. Dipali B. Tribhuvan, Diversity of endophytic fungi from Dalbergia OP 216 Aparna S.Taware and 233 sisso. Ravi P.Patil Antifungal activity and phytochemical analysis of Manisha R. Survase and OP 217 ethyl acetate extract of 234 Santosh D. Taware endophytic fungi isolated from Citrus limon. Algal flora of maize field soil in Ahmednagar Milind J. Jadhav and OP 218 235 District of Maharashtra. Swati G. Wagh Microalgal Response to Gamma Radiation and Kulkarni S.G.1, Pawar OP 219 236 Heavy Metals S.T.2 and Puranik P.R.3 Seasonal variations of phytoplankton in OP 220 Munde V.R. 237 Ambalwadi reservoir at Parli – V. Phytoplankton Diversity of various lakes in 1. Yogita L. Padme 2. OP 221 238 Aurangabad City, (MS), India. Kshama S. Khobragade Integrated management of Gloeosporium S.S. Chavan and OP 222 239 ampelophagum causing Anthracnose of grapes. V.C.Khilare INDEX

Effect of Fungicides on seed borne mycoflora of OP 223 mung bean (Phaseolus Lakde H. M., Aithal S. V. 240 aureus Roxb.) Isolation of seed borne mycoflora of Pigeon pea Aithal S.V. 1, Lakde H.M. 224 and its in vitro evaluation O/P 241 and Sherikar V.G. by some known bioagents Shaikh Sarwar Isolation, characterization and antibacterial 225 C.,Wadekar V. OP 242 property of phycocyanin N.,Kulkarni S.G. Fungal diseases of some trees from Lonar, Dist. Navalsingh J. Todawat 226 OP 244 Buldhana ( M.S.) India and P.B.Papdiwal Induction of PR-Protein in Tomato against Mustafa M. Dandu and 227 Fusarium wilt by using Biotic OP 245 Sopan B. Chavan and Abiotic Elicitors. Foldscopic Studies of the fungal endophytes of the 228 N.M.ghangaonkar PP 246 anticancer plants Population Dynamics of soil mycoflora of red rot Archana R. Mukhedkar 229 infected sugarcane field PP 247 and U.N.Bhale incited by Colletotrichum falcataum R.S.Narayankar , Status of arbuscular Mycorrhizal fungal (Amf) 230 V.R.Kumbhar, and OP 248 association in Chrysanthemum species U.N.Bhale Antimicrobial activity of crude extracts of Rahul K. Dhabale, 231 endophytic fungi isolated from Bhagwan M. Waghmare OP 249 Ventilago denticulata and Suvarna T. Kadam Mahadik S.C and Linge Study of Phyllosphere fungi of some ornamental 232 A.S O/P 250 plants

Allergenic fungal spore monitoring in some Rajurkar S.K. & 233 OP 251 intramural sites of Aurangabad city (MS). Nagwanshi M. N. Antimycotic Activity of Plant Extracts on V. Jalander and B.D. OP 234 Fusarium oxysporum f.sp. udum causing wilt 252 Gachande Disease in Pigeonpea [Cajanus cajan (L. ) Mil.sp.] Allergenic fungal spore monitoring in some Rajurkar S.K. & OP 235 253 intramural sites of Aurangabad city (MS). Nagwanshi M. N Seasonal variations of phytoplankton in O/P 236 Dr. Munde V.R. 254 Ambalwadi reservoir at Parli – V. Comparative studies of Synechocystis salina R.A Bagwan, K.D. O/P 237 255 under different physical and chemical parameters. Gopale, A.N Salve Studies on powdery mildew of some forest plants Kantule A.P.and Pawar 238 PP 256 in Gautala forest, Kannad, India S.M.. Enzymatic activity of fungi associated on fruit rot V. S. Chatage and U. N. O/P 239 257 of Coccinia indica (wight and arn). Bhale Standardization of Mushroom Cultivation on D.K. Kirwale and D.S. O/P 240 258 Agro-wastes in Marathwada Region Mukadam Theme-M Taxonomy and Plant Systematic Some new exotic plant records to the flora of OP 241 Vinod Raghoji Jogdand 260 Maharashtra Taxanomy and Diversity of Coriolopsis From the OP 242 Raibhole U.K. 261 Nanded District of Maharashtra (M.S.) India. Nut morphology of the genus Scirpus Sudhir Solanke, Raffique OP 243 (Cyperaceae): Shaikh. & Ravikiran 262 SEM study in beed District, Maharashtra, India. Sawant. INDEX

Retaining the dominancy by local flora over OP 244 Momin R. K. 263 foreign in Marathwada region. Pollen morphology of Mimosoideae family from P.Ravi Raj and A. Vijaya OP 245 Manchippa Reserve 264 Bhasker Reddy Forest, , state. Common Names of Coconut (Cocos nucifera Patil 1D.A. and OP 246 L.Arecaceae): Some 265 S.B.Shisode2 Indications On Its Nativity. Psychoactive Plants in the State of Maharashtra D.A. Dhale and D. A. OP 247 266 (India) Patil Study of some medicinal plants in Soegaon, OP 248 Deepak Devilal Pardhe 268 Aurangabad (M.S.) India Some noteworthy plant records to the flora of Kakpure M. R., More U. OP 249 269 District (Maharashtra) India A and Muneshwar A.S. Tukaram Gitte and Arvind OP 250 Anatomical studies in Tephrosia villosa (L.)Pers. 270 Dhabe Study and Enumeration the medicinal plant of OP 251 Ziya Ansari , Y. S. Patil 271 tribal areas, Nasik District Morphological anatomical and phytochemical OP 252 S. T. Chavan 272 studies on Clematis heynei M. A. Rao. Shantanu Chavan, Rupali OP 253 Dermatological studies of genus Alysicarpus Desv. 273 Taur & Dillep Pokle Occurrence of some common metropolitan trees V.B.Chavan and OP 254 in developing cities of Marathwada and 274 L.K.Suryawanshi Bioasthetic planning A brief survey of some medicinal weeds from Sangeeta S. Sutar, Chitra 255 PP 277 Aurangabad District, Maharashtra. Bagmar Smita P. Gudadhe , Morphological analysis of species of Prashant J. Gadge Varsha OP 256 278 Chlorophytum using numerical analysis S. Dhoran and Varsha N. Nathar Comparative Studies of Tribulus terrestris and T. Pathak M P and Kulkarni OP 257 rajasthanensis 279 G B Bhandari & Sharma of Zygophyllaceae Rizwan Younus Khan, N. Campus Flora of Shri Shivaji College Kandhar of J. M. Reddy, Mulani R. OP 258 Taluka Kandhar, M., Vijigiri Dinesh, Abrar 280 District Nanded, Maharashtra State, India. Baig and Jadhav Arunkumar H. Theme-N Tissue Culture Biotechnology and Nanotechnology In vitro multiplication studies in Spilanthes Algabri Ahmad and OP 259 acmella 282 Narayan Pandhure L. (Murr.) Tissue culture and pharmacognostical studies in Pallavi Prashad and OP 260 283 Datura inoxia Mill. Narayan Pandhure Shilpa Chavan, Ashwini Antimicrobial activity of important medicinal OP 261 Kasbe and Narayan B. 284 plant Adansonia digitata Pandhure Review on GMO Technology Eco-friendly to some OP 262 Sandip R.Rathod 285 Aspects INDEX

“Isolation and screening of endophytic fungi for Sayyad Shahim1, OP 263 biosynthesis of Gachande B D and Shaikh 286 silvernanoparticles” N F Rushipatna Tare and 264 In vitro callus induction in Terminalia arjuna PP 287 Sanjay Birajdar Detailed quantification of base metals and precious metals of scrap PCB of Sonawane P.M and Gupta 265 mobile phones by FE SEM/EDAX and Inductively O/P 288 S.G Coupled Plasma-Optical Emission Spectroscopy method. Green synthesis of copper nanoparticles for 1Manisha N. Chalse and OP 266 289 control of urinary tract infections 2Aniroodha V. Pethkar Yawalkar R. R. 1, Jadhav Studies on phytochemical characteristics of in OP 267 D. S. Kharat A. S. and 290 vitro callus development of Celosia argentea L. Kulkarni J.A. Solanum xanthocarpum mediated silver Rohini Pungle, Dr. Arun OP 268 nanoparticles synthesis and its 291 S. Kharat antimicrobial potential In Vitro Callus induction in Terminalia Arjuna Rushipatna Tare and 269 PP 292 Narayan B. Pandhure Aditi waghmare, Priti “Production of silver nanoparticle from fungal Taware, Poonam mycelium and its antibacterial activity against Chautmal, Dipeeka 270 PP 293 Pyogenic clinical isolates.” Potdukhe, Priyanka Mandal, Onkar Kharat & Savita Kate Isolation, characterization and antibiogram of Warkhade B.B., 271 copper resistant bacterial strain from industrial Goregaonkar S.S., Tiwari OP 294 waste water and soil. S.R. 272 Study of Plant Tissue Culture Method S.P. Gaikwad , OP 295 Optimization of fermentation conditions for L- Mohammed Mujahed, 273 glutaminase production by marine halotolerant B.M. Kareppa, Nilesh V PP 296 Pseudomonas aeruginosa MM-2 More, S.H. Tarte Additional Abstracts Study of Zooplankton Diversity in Pashan Lake, A.V.Andhale & Gunwati V. 1 Pune Maharashtra O/P 298 Arak

Some Traditional handicrafts made from plants in Hussein &Dhabe, Arvind O/P 2 299 the villages of Hajjah District in Yemen. S. Toxicity impact of Cestrum nocturnumon Jawale C. S. and Singh- O/P 3 300 respiratory metabolism of Fish Clarias batrachus Gupta S. INDEX

A New Species of the Genus Tylocephalum Linton (1890) O/P 4 From Intestine of Marine Fish Eregoodoo Ashok mote 301

Vijay A. Paithane Note on Taxonomic Identity of Grewiaheterotricha Mast. O/P 5 from India (Malvaceae -Grewioideae). & amp; A. S. 302 Bhuktar Sahasrabudhe Comparative studies of bacterial degradation of azo dye O/P 6 Direct Red 81 Madhuri 303

Mhaske S.B. and Screening, Isolation and Detoxifying of hexavalent Cr(VI) Kulkarni R. A. O/P 7 by Bacillus 304

Sonawane P.M. and An Insight into E-waste: Exploring its toxicity and O/P 8 Gupta S.G. 305 preciousness.

Pathak M. P. and Anatomical Studies of T. rajasthanensisBhandari& O/P 9 Sharma of Zygophyllaceae Kulkarni G. B. 306

Analysis of Enzyme Activity during Vermicomposting of Vishal Bedreand O/P 10 Organic Wastes (Corn cob and Cauliflower) employing Balraj Khobragade 307 the Earthworm Eudriluseugeniae

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Department of Botany, Deogiri College, Aurangabad. Page 1

Emerging Trans-Disciplinary Approaches in Bio-Sciences – 2019 ETDAB-2019 ______

Theme-A

Agriculture crop sciences and forestry sciences

Department of Botany, Deogiri College, Aurangabad. Page 2

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A-1 Utilization of Organic Manures with Inorganic Fertilizers and effects on Growth &Yield Components of Soybean (Glycine Max L. Merill.)

Kale V. R. and Salve U. S.* Sunderrao solanke Mahavidyalaya, Majalgaon, Dist. Beed. *Dept. of Botany, swa. Sawarkar Mahavidyalaya, Beed. ______

ABSTRACT The field tials were carried out to study the effect of organic manures and inorganic fertilizers on growth and yield of soybean crop (Glycine max L.). The experiment was conducted by Randomized complete Block Design (RCBD) method and soybean variety (Mahabeej) was used for cultivation. The fertilizer treatments consists of urea, 18-18, 12-32- 16, FYM, poultry manures, compost manures and control crop was without treatment. The observation revealed that the poultry manures+18-18 and FYM +18-18 fertilizers had significant effect on stem diameter, number of branches, plant height and number of leaves at 30, 60, and 90 days after sowing the crop while FYM+18-18 had significant effect on plant weight as compared to the control. The results showed that poultry manures and nitrogen fertilizers increased the number of seeds per pod as compared to the control. The application of Urea showed significant colour of plant. There was no significant difference among fertilizers treatments for pod weight per plant, number of seeds per plant.

Keywords: Soybean variety, Organic manures, Inorganic Fertilizers, Poultry Manure, Yield.

Department of Botany, Deogiri College, Aurangabad. Page 3

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A-2

Geochemistry of groundwater in an agricultural area of Hingoli, Maharashtra, India

1Salve P. L., and 2Gaikwad S.G., 2Aher K.R, 1Deputy Director, Groundwater Survey and Development Agency, GSDA, Deputy Director office, Near SAO Office, Osmanpura, Aurangabad-431001 2, Junior Geologist, Groundwater Survey and Development Agency, GSDA, Deputy Director office, Near SAO Office, Osmanpura, Aurangabad-431001

ABSTRACT The area in Hingoli district, Maharashtra is selected to discuss the groundwater quality on irrigation and human health, where the agriculture is the main livelihood of rural people and the groundwater is the main source for irrigation and drinking, therefore the study was conducted to evaluate factors regulating groundwater quality in an area with agriculture as main use. 75 groundwater samples have been collected from Hingoli District (India) for pH, EC, TDS, TA, TH, Ca2+, Mg2+, Na+, K+, CO32-, HCO3-, Cl-, SO42-, NO3-and F-to understand the sources of dissolved ions and assess the chemical quality of the groundwater. A comparison of the groundwater quality in relation to drinking water quality standards proves that groundwater quality at same places are not suitable for drinking, The correlation of the analytical data has been attempted by plotting different graphical representations such as Wilcox and US Salinity Laboratory for the classification of water, and results show that groundwater at same places also not fit for irrigation. The high values of electrical conductivity and high concentrations of Na+, Cl−, SO42− and NO3− in the groundwater appeared to be caused by the dissolution of mineral phases and would appeared to be caused by anthropogenic activities, such as intense agricultural practices i.e. application of fertilizers, irrigation practice, urban and industrial waste discharge, among others. A management plan is suggested for sustainable development of the area.

Keywords: Hydrochemistry, irrigation practice, Groundwater, Hingoli.

Department of Botany, Deogiri College, Aurangabad. Page 4

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A-3

Control of seed-borne Alternaria species using extraction of medicinal plant

Sulochana Ramsing Rathod1, Surekha Anil Saraf2, Suchita P. Bharambe3

1Dept.of Botany, 2Head, Dept.of Zoology,3 Dept.of Microbiology, Govt.College of Arts and Science, Aurangabad.

ABSTRACT

Seed-borne Alternaria species are isolated from different variety of cereals and pulses seeds. Five different variety of cereals and pulses seeds are used. Alternaria alternata is most frequently isolated species on all five type of cereals and pulses followed by A. tenuissima, A. crassa. A. triticum etc. determined by plating the seed on both agar plate method (APM) and Standard blotter paper method (BPM) and medicinal plants leaf extracts. Neem , Tulsi, Hibiscus, in different concentration of leaf extract were used from 10% to 50% concentration were evaluated against two common species of Alternaria isolated from cereals and pulses. The result revealed that all the plant extract inhibited the growth of mycelium. Effect of all type of plants were varied in various concentration of leaf extract. Whereas lowest growth inhibition record in 10% leaf extact concentration. Seed treatment at 30% leaf extract concentration in all the species are found to be effective in reducing the relative of seed-borne Alternaria species occurring on the seeds and also increase percentage of seed germination. Leaf extract of medicinal plant inhibited growth of Alternaria species on seed and useful for seed germination.

Keywords – Seed-borne Alternaria species, leaf extract, seed treatment.

Department of Botany, Deogiri College, Aurangabad. Page 5

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A-4

Impact of Chemical Mutagens on Cluster Bean (Cyamopsis tetragonoloba (L.)TAUB.)1V. R.

Mhaske and M. S. Wadikar2

1Department of Botany Shikshan Maharshi Dnyandeo Mohekar Mahavidtalaya, Kallamb (MS) 2Department of Botany Arts, Commerce and Science College, Kille Dharur. (MS) 431124.

ABSTRACT

In the present study the variety of cluster bean namely Ankur Rani was used to induce genetic variability. For these study two chemical mutagens, namely EMS of different concentration such as 0.05%, 0.10%, 0.15% and SA of different concentration such as 0.01%, 0.02% and 0.03% were used. The seeds of variety Ankur Rani of Cluster bean were obtained from Local market of Aurangabad (Maharashtra). These seeds were subjected to mutagenesis by using chemical mutagens. M1 generation was raised and effect of these mutagens on different parameters like germination percentage, seedling height, leaf morphological changes, chlorophyll chimeras, pollen sterility, plant survival percentage and morphological mutation was studied. Both the mutagens had shown an inhibitory effect on seed germination. The 0.15% EMS showed highest germination percentage. The 0.03% SA showed lowest germination percentage. Seedling height was found to increase as concentration of both the mutagens. But exceptionally it is found to be decreased in 0.03% SA concentration. In all the treatments the leaves of plants exhibited variations Bi and tri furcation of leaf lamina and Distorted leaf lamina. Survival of plant at maturity was found to be decreased at higher concentration of mutagens. The highest percentage was shown by EMS (0.05%). The lowest percentage was shown by SA (0.03%).

Department of Botany, Deogiri College, Aurangabad. Page 6

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A-5

EFFECT OF N – FERTILIZERS ON SILAGE FERMENTATION Smita Basole

Department of Botany,

Balbhim Arts Science And Commerce College , Beed. 431122. (Maharashtra).

ABSTRACT

Indian agriculture achieving self sufficiency in food grain production since from independence. In spite of having huge live stock the milk and other animal products production is not satisfactory. This is due to malnutrition and under nutrition among them. To enhance animal productivity the nutritious fodder crops, forage must grow with supplements. The supplements are fertilizers particularly nitrogen (N) fertilizer. N – fertilizer favors luxurious vegetative growth of fodder and also changes its ensilaging characters. Fertilizers application produces silage with high protein content. If fertilizers application is linked up with mechanical treatment like maceration and subsequent pressing, a good silage could be made from non-leguminous crop.

Keywords : foliages, N-fertilizer , silage ,pulping pressing

Department of Botany, Deogiri College, Aurangabad. Page 7

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A-6

STUDIES ON INFLUENCE OF LEAF LITTER COMPOSTS ON YIELD AND NUTRIENT OF BEET ROOT

Sarwade B. P.* R.P. Patil &M. A. Kare *Department of Botany, Swami Vivekanand Senior College, Mantha, Dist Jalna Department of Botany, Pratishthan Mahavidyalaya Paithan, Dist. Aurangabad

ABSTRACT The aim of the present investigation was to find out the effect of leaf litter compost prepared by aerobic (NADEP) and anaerobic (Bangalore) pit methods on Nutrient content in beet root (Beta vulgaris L.). The field experiment was conducted in research farm of Dr. Babasaheb Ambedkar Marathwada University, Aurangabad. The experiment was conducted in plots of size 150 x 150 cm with six treatments and four replications. The composts were used as NADEP, Bangalore method (compost), vermicompost and Dung treatment separately in comparison with recommended fertilizer (N40:P30:K30 Kg/ha) and absolute control. The beet root was sown by the method of ridges and furrows at the seed rate of 10 Kg/ha. The Nutrient content in beet root was estimated at 70 DAS (days after sowing). The sample of beet root was kept in oven for drying, grinded to a fine powder and it was used for the estimation of Nutrient content. The leaf litter composts increased the yield and nutrient content of beet root. Beet root are biennial plants grown as annuals and harvested for their swollen root tuber. The flesh with brown/purple skin, although yellow and white fleshed varieties are also available. While beet root is most commonly eaten as a cold, salad vegetable, pickled in vinegar and it also makes a delicious hot vegetable. Grated beet root may also be eaten raw. Beet root is utilized as a food as it is rich in nutrients like protein carbohydrates, vitamins and minerals etc. In the present investigation, application of cattle dung, NADEP compost and vermicompost increase total reducing sugar in the beet root as well as total plant. While significant reduction was observed after the application of fertilizer and many times it was less than the control. Leaf litter can be considered as a source of Agricultural waste which return directly to the soil after the formation of compost.

Keywords: - Compost, Beet root Yield and Nutrient Content.

Department of Botany, Deogiri College, Aurangabad. Page 8

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A-7 Antimicrobial Activity of Some Indian Medicinal Plants against Seed Mydcoflora

R.G. Biradar Dept. of Botany, Sambhajirao Kendre Mahavidyalaya, Jalkot,

ABSTRACT

Diseases are responsible for decreasing productivity and post-harvest deterioration of crop plants. Diseases caused by fungi are major in plants and ultimately to seeds. Hence present investigation was carried out to know the mycoflora of oil seeds and its control by medicinal plants. The antimicrobial potential of five plants was screened against four pathogenic fungi, using minimum inhibitory concentration (MIC). Different solvent extracts of Adhatoda zaylanica, Lantana camara and Sida cordifolia exhibited good activity against all the fungi tested and the MIC was recorded in range of 7.35 –32.5 µg/ml.

The present investigation will be helpful in the control of seed borne fungal pathogens of crops. Health of the seeds can be maintained and seeds can be stored for longer by using these medicinal plants. Hence the present investigation will be useful for seed health management, which is an ecofriendly approach.

Department of Botany, Deogiri College, Aurangabad. Page 9

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A-8

“BACK CROSS PROGRAMME ON SESAMUM INDICUM” Ms. Srushti S.Kale & Mr. J. D. Salunke.

Department of Botany, Dr. Babasaheb Ambedkar Marathwada University Aurangabad.431004 Maharashtra, India.

ABSTRACT The sesame botanically described as sesamum indicum.Which is a member of family pedeliaceae. Which is grown for its tender seeds in tropical and sub tropical warmer part of temperate region. China and India are the top sesame producing country in the world. Most productive sesame form located in Greece where o.69 tones per hectare of sesame production was recorded in 2013. The seed of two different varieties of sesame from local market of Aurangabad,(Maharashtra) in India. The seed of different varieties of sesame were subjected to back cross programme.

The two intraspecific variety of the sesame plant differing in their colour of seed were hybridized together. Hybridization was done. Its the process which was artificially created a variable population for the selection of types with desirable character. Hybridization, Emasculation, Back cross criteria were Studied. The reproductive phase were observed in two stages of early and late bloom. The early bloom planting takes 45-52 day. The late bloom takes 82-90 day time for flowering Crossing in between the two different varieties of sesame plant done by depositing the anther of black sesame on white sesame such emasculation and cross pollination.

Keyword:- Hybridization, Emasculation, Back cross.

Department of Botany, Deogiri College, Aurangabad. Page 10

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A-9

Evaluation of Organic Compost for Physico-Chemical Analyses and impact on Soybean and Wheat Vigour Growth under farmers’ conditions

G.M. Birajdar1, S.P.Surve2 and U.N.Bhale1*

*1Research Laboratory, Dept of Botany, Arts, Science and Commerce College, Naldurg, Tq. Tuljapur, Osmanabad- 413602, Maharashtra, India

2Department of Botany, Kohinoor Arts, Commerce and Science College Khultabad, District Aurangabad, 431101, Maharashtra, India

ABSTRACT

Organic composts contain relatively low concentrations of the actual plant nutrients and are not immediately available for plant utilization. Hence, the fortification of organic wastes and their composts as a source of organic nutrients are imperative for sustainable agriculture. This paper describes eighteen physico-chemical parameters from different organic compost. Among tested organic fertilizers, Fard Yard Manure (FYM) (56%) showed highest ash content followed by vermicompost (53.15%). pH contents found almost alkaline in nature except cow dung manure.EC was found highest in poultry manure (0.83 dS/m) while less in baggase ash (0.17 dS/m).Nitrogen content is found highest in baggase ash than other compost. In Poultary manure, Phosphorus, Potassium and Sodium are found very less as compared to other compost. The study also aimed at evaluating the effect of organic manure on the growth and yield of soybean (Glycine max) and wheat (Triticum aestivum) with six treatments each of which was replicated five times in farm field. All the growth parameters were differed significantly due to the application of organic compost. The height (56±3.29 cm) and No. of pods (243±2.97) were increased in poultry manure as compared to others treatments. In case of soybean, poultry manure, cow dung and FYM observed beneficial results among treatments. The result revealed that the treatment of vermicompost (108.68±1.47) showed significant weight of grains/ plant in wheat.

Keywords: Organic Composts, physicochemical parameters, Productivity, soybean and wheat

Department of Botany, Deogiri College, Aurangabad. Page 11

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A-10

EFFECT OF MUTAGENESIS ON SEED GERMINATION IN TWO DIFFERENT VARIETIES OF CICER ARIETINUM L.

Mrinal P. Joshi; Kishor A. Matsagar; Indraneel V. Jiwane; Seema V. Dongre and Arvind S. Dhabe

Molecular biology lab, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431001, Maharashtra, India

ABSTRACT

Effect of mutagenesis on seed germination in two different varieties of Cicer arietinum L. was studied. Varied concentrations of chemical mutagens viz. Ethyl Methane Sulfonate (EMS) 0.05%, 0.10%, 0.15% and Sodium Azide (SA) 0.01%, 0.02% and 0.03% were used. Two different varieties of Cicer arietinum L. i.e., BDNG-797 and BDNGK-798 were collected from Ariculture Research University, Badnapur, Dist: Jalna. Effect of these mutagens on seed germination was studied. Highest germination percentage was recorded along with some morphological changes. The most effective mutagen observed was EMS.

Keywords:Cicer arietinum L., mutagenesis, EMS, SA.

Department of Botany, Deogiri College, Aurangabad. Page 12

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A-11

“Nutritional Status Assessment, Effects and Evaluation of Water and Waste Water on Crops”

Bharati S. Tapase

Department of Environmental Science, Sevadal Mahila Mahavidyalaya, Sakkardara Square, Umrer Road, 440024 Maharashtra, India

ABSTRACT

The present experimental study was effective for long term additional source of water for future and present verdure era. The effect of waste water on soil fertility checking by adding and pouring water and waste water by using experimental crop and also checking nutritional status (NPK) by observing growth within experimental time. Significant change occurs when we pour water and waste water by observing crop growth. There is good growth in waste water as compared to water. Waste water reuse is good option for alleviation the ever increasing irrigation water scarcity.

In this study we have to check the effects of water and waste water on soil fertility. This information will help farmers to decide the problem related to soil nutrients amount of fertilizers to be added to soil to make production economic. This research showed that the soil irrigated with waste water has the best concentration of organic matter. The study work deals with nutritional status of the soil and assess by pouring waste water and water on experimental crop and checking its status by applying water and waste water by observing the growth. Study period was 37 days and checking the plant height in control and experimental sample.

Keywords: verdure, NPK, alleviation, growth, waste water

Department of Botany, Deogiri College, Aurangabad. Page 13

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A-12

OLIGOMERS AS NATURAL GROWTH PROMOTING AGENTS

Saoji D.D1.,Kottayi M.C1&Chaudhari A.D2

Department of Botany, P. G. T. D. Dept. of Botany, RTM, Nagpur University, Nagpur.

ABSTRACT

Alginate and chitosan are natural biopolymers. Its oligomers have potential to promote growth in terms of seedling height and early flowering in Vignaradiata, particularly low molecular weight particles generated by radiation degradation shows increase in seedling height and plant height through various seed seedling and foliar treatments. Thus alginate and chitosan are potential formulations as a new class of growth promoters towards the goal of sustainable agriculture. Generation of low molecular weight particles can easily be achieved through radiation degradation which can be explored in the field of agriculture.

Key words: Alginate, Chitosan, radiation degradation, plant growth promoters.

Department of Botany, Deogiri College, Aurangabad. Page 14

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Theme B& C

B- Aquatic Botany Aquaculture and Fishery Science C- Wild life Conservation and Forensic Sciences

Department of Botany, Deogiri College, Aurangabad. Page 15

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B-2

Toxicity impact of Cestrum nocturnum on respiratory metabolism of Fish Clarias

batrachus

Jawale C.S. and Singh-Gupta S.

Department of Zoology, HPT Arts & RYK Science College, Nashik 422005,

Maharashtra.

ABSTRACT

The effect of alcoholic extract of C. nocturnum was observed on Clarias batrachus andLC50 value was noted. The animal was exposed to 1⁄3 LC50 value, (0.933 ppm) for 48 hrs. Oxygen consumption was decreased in the treated fish as compared to the control fish. Reduced SDH and elevated LDH levels were observed in liver, gills and muscles upon exposure to piscicidal extract. This indicates that the piscicidal extract of C.nocturnum leaves impairs the respiratory enzyme activity in Clarious batrachus and hence has a lethal effect.

Keywords: Cestrum nocturnum, piscicide, LDH, SDH, Clarias batrachus, Oxygen consumption.

Department of Botany, Deogiri College, Aurangabad. Page 16

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B-3

HISTHOPATHOLOGICAL STUDY OF SENGA SP. INFECTED IN FRESHWATER FISH MASTACEMBELUS ARMATUS

Yogesh Reddy, Hariom Ingale and Aniket Karpe PG Dept. of Zoology, Deogiri College, Aurangabad 431 005

ABSTRACT

The histopathological observation were studied in the gut of freshwater fish, Mastacembelus armatus infected by Cestode parasite Senga sp. Adult parasites were located in intestinal fold of lamina propria of the freshwater fish.Forming a hole and passing through the intestinal wall and damaging the tissue which causes deterioration in the tissue, inflammation and necrosis in the tissue cell. It was also observed that the cestode, infected in intestinal layer internallygrew faster (excluding parasite weight) and maintained a similar or beter body conditioncompared with uninfected, despite developing enlarged spleens.

Keywords: Mastacembelus armatus, Senga. Histhopathology

Department of Botany, Deogiri College, Aurangabad. Page 17

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B-4 EFFECT OF EKALUX (25 % EC) ON HISTOPATHOLOGICAL CHANGES IN LIVER OF FRESHWATER FISH CHANNA GACHUA (HAMILTON, 1822) Nilesh Pakhare

Deogiri College, Aurangabad, 431005 (MS)

ABSTRACT

Alteration in behavioral patterns and histopathology of liver were studied in Channa gachua, after exposure to Ekalux (25 % EC). The 96 hour LC50 of Ekalux was determined to be 1.473 ppm. The exposed fish exhibited irregular, erratic and darting swimming and became lethargic due to inhibition of AchE activity. The liver showed rupture of sinusoids, loosening of hepatic tissue, vacuolation between hepatocytes, degeneration and vacuole formation in islets of Langerhans, shrinkage of central vein and necrosis in hepatocytes. The result of the present study revealed that Ekalux toxicity affects the liver.

Keywords: Ekalux, Channa gachua, Liver,

Department of Botany, Deogiri College, Aurangabad. Page 18

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C-1

Diversity and Conservation of Ceropegia L. from Satpuda range of Jalgaon district (M.S.)

Tanveer A. Khan

Department of Botany, H. J. Thim College of Arts and Science Mehrun, Jalgaon, Maharashtra-425001.

ABSTRACT

Jalgaon is one of the district in the region of North Maharashtra India with great diversity of plants. Jalgaon district lies between 200 and 210 North latitude and 740 55' and 760 28' East longitudes. The forest of Jalgaon district is of the tropical, dry deciduous type. The vegetation varies with the changes in altitude, aspect and rainfall. The highest peak being about 1175 meters. The genus Ceropegia L. is represented by 244 taxa worldwide while in India it is represented by 56 species 2 varieties and 1 forma. The species of Ceropegia L. are known for restricted distribution, rarity and endemism. Many of the Ceropegia L. species are known just from type localities and few are by type collections only. Many of them are under threatened categories. Genus Ceropegia L. with its curious flowers attracted the attention of botanists. During the botanical explorations of satpuda range of Jalgaon district of Maharashtra, India. Four interesting rare taxa Ceropegia bulbosaRoxb.,Ceropegia hirsuta Wight & Arn., Ceropegia oculata Hook. andCeropegia vincifolia Hook. are collected from the restricted locations and various habitats for the first time from Satpuda range of Jalgaon district of North Maharashtra India. Along with the Ex- situ and In-situ conservation of some species of Ceropegia L.

Keywords: Diversity, Conservation, Ceropegia L., Satpuda range, Jalgaon district.

Department of Botany, Deogiri College, Aurangabad. Page 19

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C-2

STUDIES IN POLLUTION CONTROLLING PLANT SPECIES OF SALIM ALI LAKE Sangeeta ahuja and narayan pandhure

Department of Botany, Sir Sayyed College, Aurangabad *Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431001

ABSTRACT

Salim Ali Lake comprised a rare and rich biodiversity spot within the city. Salim Ali Lake is very much interesting with regards to vegetation because of the fact that the floristic compositions of this locality are mixed type having both terrestrial and aquatic which are yet to be explored. No systematic and extensive floristic works on this lake have been done except for a few scattered reports. The present research deals with the selection of pollution tolerant aquatic plants species growing in the water environment of Salim Ali Lake, situated in Aurangabad District of Maharashtra. Environmental pollution has reached to such a stage that biological species are struggling for its existence. Human beings are also facing the health impacts due to increased pollution. None of the spheres of earth remain untouched by pollution. Be it hydrosphere, lithosphere or atmosphere, each one is experiencing abnormal levels of pollution. Atmosphere is polluted the most. Major sources of atmospheric pollution are industrial emissions, vehicles, construction activities, mining, dust particles and other anthropogenic activities. With advancement in science and technology, human population has also increased dramatically. The present paper deals with the selection of pollution tolerant aquatic plants growing in the water environment of Salim Ali Lake, Aurangabad. Present research work revealed the presence of 44 aquatic plants, out of which 18 species belonged to Dicotyledons and 26 to monocotyledons representing 27 families. The present paper deals with the selection of pollution tolerant aquatic plants growing in the water environment of Salim Ali Lake, Aurangabad. The systematic analysis will be carried out. According to earlier work on Macrophytes in Salim ali lake by Chavan et al. (2013) has revealed the presence of 44 aquatic plants, out of which 18 species belonged to Dicotyledons and 16 to monocotyledons representing 27 families. Keywords: Pollution controlling plant species, Salim Ali Lake, Aquatic Plants

Department of Botany, Deogiri College, Aurangabad. Page 20

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C-3 Uses of some Medicinal plants For Wound Healing In Aurangabad District M.S.(India) Manish V. Bankar

Dept.of Botany, Siddharth Arts,Commerce&Science College, Jafrabad, Dist.Jalna(M.S) India

ABSTRACT A wound is a type injury which happens relatively quickly in which skin is torn,cut,or puncturrd or where blunt force trauma causes a contusion. In pathology,it specifically refers to a sharp injury which damages the epidermis of the skin.Wound healing process is known as interdependent.Cellular and biochemical stages which are in trying to improve wound.The present ethno-botanical explorations conducted in forest areas of Ajanta region resulted in the information about traditional plants uses of 29 plant species belonging to 24 Angiospermic families.The information collected from the tribal such as Mahadeo koli, Tadvi Bhill, Laban and other village people from Ajanta region possess good knowledge of herbal drugs for treating of wounds. There is increasing interest to use the medicinal plants in wound healing because of lower side effects and management of wound over the years. Studies have shown that medicinal plants improve wound healing in diabetic, infected and opened wounds.

Keywords:-Injury, wound healing, Traditional plants, Tadvi bhill, Ajantha region.

Department of Botany, Deogiri College, Aurangabad. Page 21

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C-4

Study on Biodiversity of Higher species and its depletion causes in Ambachandi Bala Ghat Ranges Dharur Dist. Beed Choudhari S.S.

Arts, Commerce & Science College Kille Dharur Dist Beed Pin 431124

ABSTRACT The Biodiversity of the species of higher plants of Ambachandi Ghat- Balaghat Ranges at Dahrur Dist Beed in Maharashtra totally having floaral plant species have been recorded along with their scientific name, common names, families and habits attempt has been also discussed factor responsible for biodiversity depletion in the Ambachandi Balaghat ranges.

Keywords: - Biodiversity, Depletion, Balaghat Ambachandi range.

Department of Botany, Deogiri College, Aurangabad. Page 22

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C-5

TAXONOMIC STUDIES ON WEED DIVERSITY OF MARATHWADA REGION OF MAHARASHTRA STATE

R.D. Gore & V.S. Chatage* Department of Botany, Walchand College of Arts and Science, (MS), India *Department of Botany, Kai. Rasika Mahavidyalaya Deoni, Dist- Latur (MS), India

ABSTRACT The unwanted plant species growing in ordinary environmental conditions are popularlyknown as weeds. Diversity of weeds in agricultural field is mainly depend on agricultural practices such as irrigation pattern, topography, use of fertilizers, socio economic condition, spraying and diversity of habitats. Weed diversity in agricultural field having dual importance, firstly most of weeds are used a source of cattle feed and secondly various weed species are traditionally used in ayurvedic practices, in the wake of that weeds may resolves enormous loss of crop yield and low yield may directly affect on farmers economy. Now a day’s farmers are using more herbicides in crop field to control the major threat from weeds because they know weeds are dwelling the fungi, bacteria, thrips and variety of insects. Mostly the weeds are categorized in belong to the family Commelinaceae, Poaceae, Cyperaceae etc. are more dominant over the present herbicides, but they can only eradicated by following traditional practices. Generally, they are primary competitors for the crops, lawn, orchard, gardens etc. hence these are the hazardous to creating major problem which decrease the economy, hence the proposed study carried out in 2016-2019 to study weed diversity of the Marathwada Region of Maharashtra State. During the field extensive field exploration we collected the 168 weedspecies from Marathwada region in respect of detailed morphology, flowering and fruiting season, weed type, present occurrence, ecological notes and medicinal importance. Among them about 20 weed species are rare from agricultural fields. The weeds of the region generally classified into two categories viz. monsoon weed and autumn weeds. Weed flora is having luxurious growth with habitual diversity in monsoon season due to enough rainfall and availability of moisture in soil but in autumn they are weaker and showing less diversity.

Keywords: Weed Science, Flora, Marathwada, Agriculture, Ayurveda, Maharashtra.

Department of Botany, Deogiri College, Aurangabad. Page 23

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C-6

Awareness regarding the conservation of Biodiversity illiterate peoples: Aundha region.

Dr.Pratap V.Deshmukh Dr.V.S.Maske Nagnath Arts, Commerce and BahirjiSmarak Mahavidyalaya Science College Basmathgar Dist- Hingoli. Dist- Hingoli.

ABSTRACT This paper deals with the study of study of biodiversity of Aundha region in Marathwada. Lot of biodiversity is located in the Hingoli district,i.e.first spot means Aundha. As Aundha is a Holy place no of pilgrims visit to this place.So it is our duty to know about the biodiversity of this region. Aundha is a tahasil place of Hingoli district of Marathwada(Maharashtra).Aundha has been attributed with natural buty.Aundha is covered by forest area with thick vegetation which contain no of variety of plants like herbs,shrubs,climbrs,trees etc. This diversity of plants attract the pilgrims and peoples.In this forest flora consists of large no of Medicinal Plants now a day also Abrus precatorius,Aclypha indica,Annona squamosa, Barleria cristata,Butea monosperma,Buchnania lanzan,Cleome viscose,Clitoria ternatea,Cocculus hirtus,Clerodendron serratum,Digera muricata, Dioscorea bulbifera, Dolichandron fulcata, Evolvulus alsinoides,Emblica officinalis, Ficus bengalensis, Gloriosa superba, Helicterus isora etc.Also there is diversity in fauna which consists of animals lild cat,wild pig, fox,deer, Peacock,eagle,crow ,sparrow, different types of snakes,frogs etc. But in some part of this region there is cutting of valuable plants and hunting of some animals and birds,also by some natural scarsity of water ,food which lead to migration or death.Due to this bad activity of humanbeing and natural events, biodiversity of this region becomes loose which is very hazardous for this area. For this we want to take a step to aware the peoples and Government must be involved for the protection of biodiversity.

Keywords- Biodiversity, Forest ,Vegetation, Pilgrims

Department of Botany, Deogiri College, Aurangabad. Page 24

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C-7

Diversity, Conservation Status and Medicinal Plants of the Euphorbia

*Dr. Ghadsing P.G ** Dr.I.B.Salunkhe

* Shri. College, Majalgaon** Sunderrao Solanke College, Majalgaon.

ABSTRACT

In India, the use of different parts of several medicinal plants to cure specific ailments has been in vogue from ancient times. The indigenous system of medicine namely, Ayurvedic and Unani, has been in existence for several centuries. In modern times, plants have a prominent place as raw material for some important drugs. Synthetic drugs are effective in controlling different diseases but these synthetic drugs are out of reach of millions of people. It is estimated that around 70,000 plant species have been used for medicinal purposes. Medicinal plants have curative actions due to the presence of complex chemical constituents. and pharmacological actions of Euphorbia.

Euphorbia is the largest genus of the family Euphorbiaceae with about 1600 species. All species of Euphorbia exude a milky juice. Out of these sps, Euphorbia hirta Linn. Is an annual medicinal weed and it is commonly known as Asthma plant. It is not only a weed but also it is a medicinal herb too. These medicinal herbs are found in tropical and temperate parts of the world. Extensive literature studies suggested that the various parts of the plant are reported to possess antimicrobial, anti-diabetic, anti-cancer, anti-tumor, anti-plasmodia, anti- fertility, wound healing, anti-inflammatory, sedative, Anti- asthmatic, Anti-Ulcer, and diuretic properties. The present review study deals with the botanical description, phytochemical, pharmacological, therapeutic, nutritional and other important biological aspects of Euphorbia hirta. The main phytochemicals found in this plant are polyphenols, flavonoids, steroids, tannins, and alkaloids. This review study may be utilized for the identification and preparation of a monograph of Euphorbia hirta.

Department of Botany, Deogiri College, Aurangabad. Page 25

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C-8

Biodiversity and limnological Profile of Masoli reservoir from of Maharshtra

Ahirrao S.D.

Department of fisheries, Shri Shivaji College, Parbhani

ABSTRACT

Masoli reservoir isgeologically located on 18.8978922°N and 76.7454323°ECoordinates. It is a earthfill dam on Masoli river at taluka of Parbhani district in the state of Maharashtra in India. The reservoir was built in 1981. The height of reservoir is 24.84 meters, length is 1086 m. and volume of reservoir is 626 Km3 Gross storage capacity is 34080 Km3 while surface area is 6970 Km.3 In the present investigation the seasonal Samples were analyzed by establishing the sampling station at different sites of the reservoir. The studied parameters were found in the range such as temperature (23 - 24 0C)., pH (7.3 - 8.3), dissolved oxygen (4.2 - 7.8 mg/l), free carbon dioxide (3.7 -10.62mg/l), chloride (40.4 -75.0 mg/l), total dissolved solids (204- 399 mg/l), total hardness (58.43 – 144mg/l), calcium hardness (472 – 258 mg/l), magnesium hardness (1.68 - 27.13 mg/l). In the present study the water quality is found suitable for fish culture with the diversity of various groups of fishes such as of Food fishes Larvivorous fishes, weed fishes, Predatory fishes, Migratory fishes, air breathing fishes and Forage fishes.

Keywords:-Biodiversity, limnology, Masoli reservoir and Parbhani.

Department of Botany, Deogiri College, Aurangabad. Page 26

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C-9

Current Field Status, Diversity and Distribution of AcanthephippiumBl. (Orchidaceae) in Darjeeling Himalaya of West Bengal, India

Rajendra Yonzone

Department of Botany, Victoria Institution (College), 78 – B, Acharya Prafulla Chandra Road, Kolkata – 700009, West Bengal, India.

ABSTRACT

Orchids are highly specialized and a mesmerizing group of flowering plants and are classified into four different habitats viz., subterranean, saprophytic, terrestrial and epiphytic. Sometime, epiphytic species found in lithophytic habitat. Orchid species are widely distributed from the Equator to the Arctic Circle and from lowland areas to snowline. About 1,300 species with 140 genera of Orchid species are found in India. The main Orchid rich habitats in the country are the Himalayas, North Eastern, peninsular and the Darjeeling Himalayan regions of India. Orchids are characterized by pollination mechanism, diverse floral morphology, floral colour combination, association with special fungal associates and endospermless seeds and are considered to be the most highly evolved among the monocotyledons plant group and they exhibit an unbelievable multiplicity in structure, shape, size, colour and aroma of flowers and are attractive customary among the professional and recreational Orchid lovers of the world. It is estimated that over 22,500 species with 779 genera are distributed throughout the world. This attempt is the first step to correct taxonomic identification to workout currently accepted botanical names with photographs, habitat, altitudinal ranges, phenology, current field status, diversity, threats, local and general distribution of terrestrial Orchid species Acanthephippium Bl. (Orchidaceae) viz., Acanthephippiumsylhetense Lindl., and A. striatum Lindl., in Darjeeling Himalaya of West Bengal, India.

Keywords: Orchidaceae, Acanthephippium,Current field status, Diversity, Distribution, Darjeeling Himalaya.

Department of Botany, Deogiri College, Aurangabad. Page 27

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C-10

Biodiversity of Phytoplanktons Of Chandrapur Dam, Parli- Vaijnath Dist, Beed Maharashtra, India

VidyaDGulbhile

Department ofZoology

L. L. D. M. College Parli Vaijnath

ABSTRACT

The Presentpaper deals with the studyof biodiversityofphytoplankton inChandapur dam, Parli VaijnathDist –Beed [M.S.] India.Planktonarethemicroscopicandaquaticfor msofani mal sandpl antt hat floati aquatic env i r onment .Pr i mar i l ypl ankt onl i v ei n t hesunnyzoneoft heaquat i ceni v or onment , ev ent houghsomespeci esar ef oundi n muchdeeperwat er .Somepl ankt onpl ayani mpor t antr ol et omai nt ai nf oodchai ni nt he aquat i cbal ance.Ther ear et wot y pesofpl ankt onphy t opl ankt onandzoopl ankt on. Phy t opl ankt onar epl antl i keandt heyobt ai nedt hei rener gyi nt hef or mofcar bohy dr at es byphot osy st hesi s.Zoopl ankt onpossesani mal l i kechar act er sandcansomet i mesget v er yl ar ge.

Thepr esentwor kwascar r i edoutdur i ngt hey ear 2017-2018Januar yt oDecember . Tot al 14speci esbel ongi ngt o4di f f er entcl assessuchasChl or ophy ceae[ 06] , Cy anophy ceae[ 4], Baci l l ar i ophy ceae[ 03] , Eugl enophy ceae[ 01]wer ef ound.

Keywords: Bi odiversity , Phytopl ankt on, Chandapur dam, Par l i Vai j nat

Department of Botany, Deogiri College, Aurangabad. Page 28

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C-11

PROTECTION OF PLANT VARIETIES IN INDIA

Bandewar S.T and T.D.Kamble Department of Botany Rajarshi shahu Arts Commerce and Science College Tq.Phulambri Dist.Aurangabad.

ABSTRACT

In order to provide for the establishment of an effective system for protection of plant varieties, the rights of farmers and plant breeders and to encourage the development of new varieties of plants it has been considered necessary to recognize and protect the rights of the farmers in respect of their contribution made at any time in conserving, improving and making available plant genetic resources for the development of the new plant varieties. Moreover to accelerate agricultural development, it is necessary to protect plants breeders' rights to stimulate investment for research and development for the development of new plant varieties.Such protection is likely to facilitate the growth of the seed industry which will ensure the availability of high quality seeds and planting material to the farmers. India having ratified the Agreement on Trade Related Aspects of the Intellectual Property Rights has to make provision for giving effect to Agreement. To give effect to the aforesaid objectives the Protection of Plant Varieties and Farmers' Rights Act, 2001 has been passed in India.For the purposes of this Act, Protection of Plant Varieties and Farmers' Rights Authority has been established and is located at New

Key words :PPVFRA, IPR etc

Department of Botany, Deogiri College, Aurangabad. Page 29

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Theme - D Ecology and Eco -toxicology

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DEGRADATION OF CELLULOSE CONTAINING EFFLUENT

BY TRICHODERMA

NAYAB ANSARI

Dept of zoology, Sir Sayyed College, Aurangabad

ABSTRACT

Water pollution due to paper and pulp industry is becoming more and more hazardous, the industrial effluent contains cellulose which is depositing in the water bodies currently there are two pathways for converting cellulose into glucose they are, chemical verses enzymatic. Trichoderma a fungus produces an enzyme cellulase complex which can hydrolyse cellulose to glucose thus reducing the water pollution to some extent. In the present investigations the purified cellulase enzyme complex was immobilized and used also the enzymatic degradation was compared with the chemical degradation. It was found that enzymatic degradation was more efficient and the enzyme retained its efficiency till 72 hours and then declined. Thus cellulose a major pollutant can be converted to glucose which can be used as an energy source

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D-2

Physicochemical Water Analysis of Bindusara river in Beed District,(M.S)

Dr.Dhond G.M., Jadhav R.L.

Dept. of Zoology, Shri Siddheshwar Mahavidyalaya, Majalgaon, Dist. Beed

Dept. of Chemistry, Swa. Sawarkar Mahavidyalaya, Beed

ABSTRACT

Water is essential component of environment and it sustains life on the earth. In our country (India) fresh water resources is available in the form of River, Reservoirs, lakes and tanks etc. Bindusara river is passed through the Beed city. Bindusara Dam is located few kilometer away near the Beed city. The dam water is used for the drinking and irrigation purposes in this region. Water has important role in living things like animals, plants and human being for their existence. Physicochemical analysis of water is done for the status of water pollution in the span of the 1st November 2019 to 10th December 2019 at Bindusara

Pali lake and river water. The present investigation is carried out with analysis of some physicochemical parameters of Bindusara Pali lake water and river water in the Beed city area. The physico-chemical parameters of water such as temperature, color, Taste, odor, PH ,

BOD, COD, Total Hardness, TDS etc.

Keywords:-Bindusara dam, Bindusara river, Physicochemical parameters etc.

Department of Botany, Deogiri College, Aurangabad. Page 32

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D-3

“Effect of air pollution on chlorophyll content of Terminalia procera and Magnifera indica”

Dr. Rajmane S.D.

Shivchhatrapati College, Aurangabad.

ABSTRACT

In the present investigation, comparative studies have been done, to find the effect of air pollutants generated from the exhaust of industries and automobiles on the chrorophyll content of leaves. The leaves samples of healthy and polluted leaf of Terminalia procera and Mangifera indica were collected and chlorophyll a, chlorophyll b and total chlorophyll were quantitified. A reduction in photosynthetic pigments of polluted leaves is compared with healthy leaves.

Keywords : Chlorophyll a,b and total chlorophyll, Air Pollution, Quantification.

Department of Botany, Deogiri College, Aurangabad. Page 33

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D-4

Physico-Chemical analysis of Soil samples in Beed District, Maharashtra.

V.V.Naiknaware

Dept. of Chemistry

Swa. Sawarkar college, Beed. ABSTRACT

Yield of the crops and growth regulation fertility of soil is most important but today’s scenario of agriculture farming in India is not care about it. The present study has been undertaken to investigate the physico-chemical characteristics of soil samples collected from different villages of Beed district, Maharashtra, India. The soil characterization was carried out for the parameters like PH, Electrical conductivity, Total organic Carbon, Nitrogen,

Phosphorous(P2O5),Potassium(K2O).This study leads us to the conduction of the nutrients quality of soil of different villages of Beed district. The present study result shows that average all the villages of Beed district have medium or high minerals content. This information will help farmers to solve the problems related to soil nutrients amount of which fertilizer to be added to increase the yield of crops.

Keywords:- Soil samples, Organic carbon, Nitrogen, Phosphorous, Beed District.

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Theme - E

Economic botany Zoology Microbiology and biotechnology

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E-1

REDESCRIBED NEMATODE SPECIES OF PROCAMALLANUS ARNAI (LAKSHMI I.R., 2010)IN MASTACEBELUS ARMATUS (LACEPEDE, 1800) IN SINA KOLEGOAN DAM, OSMANABAD DISTRICT (MS)

Swati Jadhav1, Atul Humbe2 and Nitin Padwal3

1 Department of Zoology, R. P. College, Osmanabad (M.S.),India 2 Department of Zoology, S. G. R. G. Shinde College, Paranda. Dist Osmanabad (M.S.) India 3 Department of Zoology, S.P. College, Bhoom Dist Osmanabad (M.S.) India

ABSTRACT The present investigation was carried out on nematode parasites in Mastacembelus armatus (Lacepede, 1800 of Sina Kolegoan Dam, Osmanabad District. The nematode parasite Procamallanus arnai (Lakshmi I.R., 2010) was described from the intestine of fresh water fish Mastacembelus armatus (Lacepede, 1800) from Sina kolegoan Dam dist Osmanabad (MS), India during January, 2011. Procamallanus arnai(Lakshmi I.R., 2010) characterized by having the mouth is oval in shape; head bears two pairs of sub median papillae, buccal capsule cylindrical, oesophagus is of muscular anterior and posterior glandular, presence of caudal alae, seven pairs of caudal papillae are present, Spicules are unequal in size and dissimilar in shape

Keywords: Procamallanus arnai (Lakshmi I.R., 2010), Mastacembelus armatus (Lacepede, 1800), Sina Kolegoan Dam.

Department of Botany, Deogiri College, Aurangabad. Page 36

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E-2

The role of enriched supplementary feed in relation to growth of Labeo rohita.

Shaikh H.M. Patel Y.E. Department of Zoology, Iqra H.J.Thim college of Arts and science,Mehrun, Jalgaon.

ABSTRACT

The different kinds of Supplementary and usual feeds have been used for the proper growth of fishes in the practice of freshwater fish culture. Two types of feeds generally used in the mixed form with various combinations and proportion to enhance the growth of fishes in fresh water fish culture. For proper growth of fishes the balance diet must be enrich with 40%proteins,20%carbohydrates and 6% fats and proper proportion of essential vitamins.

In present work fresh water major carp Labeo rohita was selected for the study which deals with role of enriched Supplementary feed in relation to growthv .Healhty fishes were collected from local dam and randomly divided into two groups released in to A and B tanks. Fishes in tank A surved as control and tank B as experimental fish.Both tanks fishes feeds enriched supplementary feeds in equal proportions once a day in morning.

Keywords: Enriched,Supplementary feed,fish culture,Labeo rohita.

Department of Botany, Deogiri College, Aurangabad. Page 37

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E-3

Optimization of Exopolysaccharide production by marine haloalkalotolerant Psudomonas aeroginosa using response surface technique.

1Chudiwal A.B . and Dharmadhikari .S.M.2

1Microbiology Department, Shivchhatrapati College, Aurangabad, 2Microbiology Department Government Arts and Science College, Aurangabad

ABSTRACT

Evaluating an optimal conditions for growth and exopolysaccharide production has been carried out using laboratory isolated marine by Psudomonas aeroginosa . The sequential statistical methods were used to maximize exopolysaccharide production. Initially, a Plackett- Burman design was used to optimize the variable i.e. nutritional components such as four carbon sources, four nitrogen sources ,four metal sources, out of which glucose ,ammonium sulfate, magnesium sulfate sodium hydrogen phosphate significant for exopolysaccharide production (P < 0.05). Further investigation of effect of selected four nutrients using a Response Surface Methodology (second -order central composite design (CCD) was done to optimize exopolysaccharide production, which was adequately approximated with a full quadratic equation obtained from a two-factor level design. The analysis of quadratic surfaces showed that glucose ,ammonium sulfate, magnesium sulfate sodium hydrogen phosphate has significant effect on exopolysaccharide production (P < 0.05 ).The determined (R2) value was 0.97 and adjusted R-squared 0.94 , indicating a good fitted model for EPS production. The F-test applied for analysis of variance (ANOVA), F value was found to be 34.73 explain the good correlation of independent variables . Validation of the experimental model was done where maximum exopolysaccharide production (4.52 g/l) was obtained with 1.14% glucose ,0.72% ammonium sulfate, 0.054 %magnesium sulfate,0.08 % sodium hydrogen phosphate . In these conditions, the maximum EPS yield was 4.52 g/l which was 2 fold in increase as compared to basal medium .

Keywords: Exopolysaccharide, Response Surface Methodology, Psudomonas aeroginosa.

Department of Botany, Deogiri College, Aurangabad. Page 38

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E-4

Analysis of some domestic species and their products in Ajanta hill ranges with reference to traditional medicine for health care in rural population. S. T. Naphade, P. S. Patil and S. R. Naphade* Department of Zoology, Yeshwantrao Chavan College of Arts, Commerce and Science, Sillod, Dist: Aurangabad (M.S.) India. *Department of Zoology, Dagdojirao Deshmukh College of Arts, Commerce and Science, Bajajnagar, Waluj, Aurangabad (M.S.) India.

ABSTRACT

This communication deals with the study of importance and use of domestic species of animals and birds in traditional health care system in Ajanta hill ranges of Aurangabad district in Marathwada region. During this research, the study area assessed the common knowledge of rural peoples regarding the domestic species in health care system. It is carried out through the standardized questionnaire, interviewing people from different villages from a total of 186 family background and socioeconomic status during this research. Three species of animals and two species of birds with domestic value, which are important and used in rural peoples for primary health care as well as for other purposes also. Meat, milk, urine and blood are most commonly used to treat common disorders such as hair crack bone fracture, cough, asthma and wound healing on the outside surface of the skin. Such findings are made in the study area that are used as a local remedial measures. It is therefore necessary to make rural people aware of the problems of the use of these animals, birds and their products against certain primary health issues. From the above analysis and observations it is clear that such domestic species are of use to humans and are significantly help to conserve them. Other related aspects discuss in the text.

Keywords: Domestic species, Ajanta, Traditional medicine, Health care, Rural population.

Department of Botany, Deogiri College, Aurangabad. Page 39

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E-5

Coccidiosis in Backyard Poultry Chicken (Country Chicken) in Vaijapur Tehsil of Aurangabad District (M.S.)

Bhimrao N. Jadhav

VINAYAKRAO PATIL MAHAVIDYALAYA VAIJAPUR DIST. AURANGABAD

ABSTRACT Chicken coccidiosis cause major economic loss of every poultry owner. Eimerian species of phylum apicomplexa causes great threat to chicken of various ages. Various species of Eimeria causes coccidiosis in chicken. Present investigation undertaken to know status of coccidiosis, from January 2019 to June 2019. 305 samples of dropping were collected of different indigenous chicken from different species reared in farm. From 25 villages of Vaijapur samples were collected for examination. Out of 305 samples 104 were positive for coccidial infection, the percentage prevalence being 34.09%.

Keywords: Backyard poultry, coccidia coccidiosis Eimeria etc.

Department of Botany, Deogiri College, Aurangabad. Page 40

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E-6

A NEW SPECIES OF THE GENUS TYLOCEPHALUM LINTON (1890) FROM INTESTINE OF MARINE FISH EREGOODOO

Ashok mote1 S. R. Gaur1 V D Suryawanshi1

Dept. of Zoology

Sant Ramdas College Ghansawangi

ABSTRACT

The present communication deals with the new species of the genus tylocephalon Linton (1890) tylocephalon nandeoi. The present new speciesT.nandeoviis having the different character. Scolex large, globular, accessory suckers medium, neck short, mature segment small longer than broad testis small round172,175, cirrus small, vasdefference thin, short, straight, ovary small bilobed, vagina wide, tubuler, ootype post ovarian, genital pore small oval, vitellaria granular.

Department of Botany, Deogiri College, Aurangabad. Page 41

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E-7

Monitoring of Blood Group ABO & Rh(Rhesus Blood Groups)Distribution of One Year Survey in Anjangaon Surji Region Dist-Amravati - Maharashtra

M.R. Yeotkar Department of Zoology Smt. Radhabai Sarda Arts, Commerce and Science College Anjangaon Surji

ABSTRACT

This study was aimed to identify the distribution patterns of ABO and Rh-D blood group among the population of Anjangaon Surji city corporation area in Amravati city of Maharashtra in order to promote social awareness, and safe blood transfusion among the population. A cross sectional, analytical study was carried out on a total of 200 people in three different area of Anjangaon Surji Corporation .The ABO blood group system in the total sample showed the same trend of prevalence with that of the general Indian subcontinent (B > O > A > AB). The same trend was found among males, but among females the order of prevalence was different (O > A > B > AB). Rh-D positive were 90.72% and Rh-D negative were 9.28%. Study of blood grouping is not only generating a simple database but also create a great social awareness about self-blood grouping and safe blood transfusion among the population of a country.

Keywords: Anjangaon surji, Blood groups, ABO and Rh blood group system

Department of Botany, Deogiri College, Aurangabad. Page 42

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E-8

Study of Garlic (Allium sativum) effect on control measures of land snail, Macrochlamys petrosa. Deshmukh P.S. Postgraduate Department of Zoology, Government Institute of Science and Humanities ,Amravati.

ABSTRACT The land snails and slugs are important to man as a pest because of damage caused in agriculture. The present snail Macrochlamys petrosa is moisture loving and feeding actively during monsoon and winter seasons of the year. For the control purpose of this snail it is essential to understand in detail Ecobiology of these snails. The control of Macrochlamys petrosa was checked in the laboratory by conducting experiments under laboratory conditions .This experiments were carried out by using garlic effect for assessing the toxicity. Fortunately, nature has a simple solution. Allicin is a defense compound created by garlic bulbs which both repels and kills slugs and snails. Garlic is an important vegetable extensively cultivated in India. It is well known for its pungent spicy flavor. When garlic is sprayed on plants, its odor and taste deter snails from eating the foliage. When the snails come into contact with the oil in garlic, they begin to secrete more mucus. Over time, the snails will die as they dry and shrivel up from too much mucus secretion. It is observed that direct dusting of the powder on snail and sprinkling the liquid concentration of these chemicals on food of the snail is most effective as compared to mixing the chemical with soil.

Keywords: Macrochlamys petrosa,pestiferous, garlic (Allium sativum)

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E-9 Study of fresh water fish diversity of Phulmabri Dam, Aurangabad. (M.S).

Rajni S. Amrawkar and Phulwade Durgesh N.

Yashavantrao Chavan institute of Science, .

Shri Sant Savta Mali Gramin Mahavidyalya, Phulmabri, Aurangabad.

ABSTRACT

Fish form the most diverse group of vertebrates. Globally nature as well as animal diversity are affected due to increase in unwise anthropogenic activities. Aquatic ecosystem is also adversely affected due to release of wastes in it. In the field of Ichthyology there is valuable contribution by many workers. As far as economic importance is concerned, the scope of fish and fisheries in Maharashtra is of prime interest (Pawara Ravindra H., Patel Nisar G. and Patel Yusuf E. 2014). Present study on ichthyofauna of Phulambri Dam of Aurangabad district in Maharashtra was carried out 2018-2019.

Keywords: Ichthyofauna, diversity, Cyprinidiae, fresh water fish.

Department of Botany, Deogiri College, Aurangabad. Page 44

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E-10

A new species Lytocestus parturii of the genus Lytocestus (Cestoda: Lytocystiodae, Hunter, 1927) from the fresh water fish Clarius batrachus at. Partur Dist. Jalna. (M.S.), India.

R. G. Pradhan and *R. M. Dhere Department of Zoology, Lal Bahadur Shastri Senior College, Partur Dist. Jalna * Department of Zoology, Swa. Sawarkar College, Beed.

ABSTRACT

The present investigation deals with the study of new species of genus Lytocestus (Cestoda: Lytocystidae) from the fresh water fish Clarius batrachus at Partur Dist. Jalna. The worm after the keen observation found that body elongated and dorsoventrally flattened. Scolex smooth, unarmed, rounded and stumpy. Posterior end blunt and rounded. Excretory pore terminal. Testes numerous. Vitelline follicles oval. Ovary is bilobed. Uterus very well developed and eggs oval with thick shell.

Keywords: Scolex, Vitelline, Flattened, Segmentation, Clarius batrachus.

Department of Botany, Deogiri College, Aurangabad. Page 45

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E-11

Application of immobilized beads of Ps. aeruginosa 4442 and role of

various elutants on Cr (VI) recovery from metal containing solution

Kulkarni R. A. and Gupta S. G.

Government college of Arts and Science, Aurangabad, Maharashtra 431001

Government Institute of Science, Aurangabad, Maharashtra, 431001

ABSTRACT

Industrial activities and deliberate and accidental discharges are the major causes due towhich microbes are exposed to toxic levels of metal pollutants and they acquire resistance to these metals for their survival using various mechanisms like adsorption, accumulation, enzyme catalyzed transformations etc, So the microorganisms can be used to concentrate, accumulate and recover such precious metals. In this paper Ps. aeruginosa4442 is used to accumulate the hexavalent chromium from the metal containing solution. To recover this metal from the bacterial cells efficiently these microbial cells are immobilized using Na- alginate. It was found that beads containing microbial cells accumulate maximum 68% of Cr (VI) after 2 and half hours of incubation at 300 C. Various elutants were used to recover this accumulated chromium and EDTAwas found to be the most effective giving recovery of 79% of Cr (VI). These immobilized beads can beefficiently used for 5 sorption and desorption cycles.

Department of Botany, Deogiri College, Aurangabad. Page 46

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E-13

Optimization of Metallo-Collagenase Production by Arthrobacter creatinolyticus KP015744 zzx28Isolated From Deteriorated Leather Sample

Savita Kate*&Archana Pethe

* Department of Biotechnology, Shivchhatrapati College, Aurangabad-431001,M.S., India Department of Microbiology, Shivaji College of Arts, Commerce and Science,-444001,M.S., India

ABSTRACT Arthrobacter creatinolyticus KP015744 zzx28 among 26 isolates, from deteriorated leather samples was found to be most proficient collagenase producer. By using 2% inoculum,833.06±19.04 U/ml of collagenase production shown at optimum temperature 37°C after 72h and at pH 7.5 using 1% collagen peptide type I as a substrate. In presence of Hg+2, EDTA and β-mercaptoethanol the collagenase production by the isolates was strongly inhibited however Fe2+, Ca2+and DMSO act as an enhancer. Specific activity was found to be 19.46X103U/mg and molecular weight 66kDby SDS PAGE. Isolate also has a potential to hydrolyzed other leather main protein keratin. Investigational outcome propose that exploitation of biocatalyst in collagen, keratin rich solid waste Treatment.

Keywords: Leather, Collagen peptide type I, metaloprotease, EDTA, dialysis, feather meal

Department of Botany, Deogiri College, Aurangabad. Page 47

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E-14

Planktonic Biodiversity and its importance role in fish production on Majalgaon Dam reservoir, Maharashtra State. India.

Sitaram B. Ingole, Prashant Sanghai* Shri Siddheshwar Mahavidyalaya Majalgaon, Dist Beed. M.S. India. *Shivaji Arts Comm. & Sci. College , Kannad, Dist. Aurangabad. M.S. India.

ABSTRACT Majalgaon Dam was constructed on the River Sindphana which is a tributary of River Godavari, in Beed District (Maharashtra, India) in 1987.The River Sindphana has been under constant threat of pollution by sewage and industrial wastes, disposal of dead bodies, deforestration, excessive useof fertilizers and pesticides, bathing and water development programmes. The dam has a catchment area is 3840 sq. km. It is of great Importance for the region because its water is used for human and cattle consumption,power generation, fish production and irrigation. A total of 33 species of phytoplanktons, 29 species of zooplanktons and 11 species of fishes were identified. The importance of plankton in fisheries is well established. It has been clearly demonstrated that the zooplankton constitute the only food for the fish fry and the adult fish not only eat them, but also select them as a delectable item.Thus zooplankton have a direct bearing in the fish industry. In India, several studies were conducted in reservoirs elucidating the characteristics of zooplankton.The zooplankton peak was found during summer followed by winter and rainy season.Microfauna (zooplankton) was observed about four groups as Rotifera observed about eleventh species, Copepoda observed about nine species, Cladocera observed seven species and Ostracoda observed about two species. The macrofauna or fish fauna were observed at the Majalgaon Dam reservoir. There are culture of fish with quick growing varieties of fishes including Indian Major Carps, exotic species have been popular in recent time. There is abundance of the species such as Labeo rohita, Cirrhina mrigal, Catla catla, Cyprinus carpio, Silver carp, Wallago atta, Mystacenbelus armatus, Notoptemus chital, Puntues ticto, Channa staitus, Mystus seenghala, Mystus cavaassius, Eutroplus suratensis, Belon concila, Chela, Tilapia Mossambica, Rohtee alfrediana, Gobius giuris, etc.Fish is economically a very important group of animals beside being used as food. Fish liver is an important source of oil containing Vitamins A and D, several minerals and protein.

Keywords:Phytoplanktons, zooplankton, pollution, fish production.

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E-15

Avifaunal Diversity Found In and Around Dnyanganga Abhayaranya of Buldana District (M.S.)- A Report Kakde Vandana R. Department of Zoology Jijamata Mahavidyalaya, Buldana. – 443001 (M.S.)

ABSTRACT Present study deals with the observation of bird’s biodiversity in and aroundDnyanganga Abhayaranya, Buldana District,Maharashtra. Study was carried out during the months of December 2017 to November 2019 to Theresult showsthat total 94birds' speciesof16ordersand36familieswererecorded.Maximum diversity of birds occurs in the Month of October to February and lowers in the month of May to July. If natural calamitiesdoesnotaffectandhuman interferencerestrictedmorespeciesofbirds may berecordedinthisregion.Itisoursolemndutytoprotectandnurturethispreciousgiftof nature. The Birds observed are resident, migratory, local migratory and uncertain. Feeding habitat is observed as frutivorous, insectivorous, grainivorous, herbivorous, carnivorous, nectar suckers and omnivorous. Sincenodatapertainingtoavifaunaofthisregionisavailableitwasdecidedto prepareachecklistofbirdsalongwiththeirapproximatepopulation.

Keywords: Biodiversity, Birds, precious, frutivorous, insectivorous, grainivorous, herbivorous, carnivorous, omnivorous, conservation etc.

Department of Botany, Deogiri College, Aurangabad. Page 49

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E-16

Protein Estimation in the Body of Tetragonocephalum Pulensis From A Marine Water Fish Trygonsephen

Dr. Nilima M. Kankale1, Dr. Varsha M. Jaisingpure2

1Assistant Professor, Department of Zoology, G.N.A. Arts, Commerce & Science College, Barshitakli, Dist. Akola, (M.S.), India

2Balbhim College of Arts, Commerce & Science,Bid, Dist. Bid, (M.S.), India

ABSTRACT

Cestodes are endoparasites found attached to the Wall of the intestine. They are finally attached the host intestine through their suckers and hooks of the rostellum. The remaining body is freely held in the lumen of the host intestine. The naked covering of the body of the parasite is permeable to physiological substances. Proteins are the most abundant organic molecules in the cells containing 50% or more of their dry body weight. They are found in every part of every cell, since they are fundamental in all aspect of cell structure and function. There are many different kinds of proteins each specialized for a different biological function. The Tetragonocephalum Pulensis is the scolex divided into two mature segments are longer than broad; Testis are Preovarian, ovary is bilobed and H shaped, Vitellaria are granular arranged in 2 or 3 rows.

Department of Botany, Deogiri College, Aurangabad. Page 50

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Effect Of Sodium Fluoride On Vas deferens of Male Albino Rats (Rattus norvegicus) Sudheer S. Senigarpu M.Sc*, Dr.V.T.Dhurvey PGTD Department Of Zoology, Mahatma Jyotiba Phule Educational Campus Under RTM Nagpur University, Nagpur.

ABSTRACT

The vas deferens also called ductus deferens is part of male reproductive system of many vertebrate. The vas deferens is a tube like as a epididymis. The muscular layer of the vas has three layers a thick intermediate circular layer of muscles fibers and inner and outer longitudinal layers. The muscular layer of the vas helps in propelling sperm through the duct system. There is evidence in the literature that, sodium fluoride (NaF) is a poisonous toxic compound in the environment naturally and as poisons emanating from various ways. Long et al. (2009) reported the current scientific understanding of the links between environmental exposure to fluoride (F) and its known or potential effect on human male fertility. Chinoy et al.(2005)reported the combined administration of sodium fluoride (NaF, 10 mg/ kg bw /day)+aluminium chloride (AlCl3, 200 mg/ kg/ bw/ day) to adult male mice for 30 Days caused histological changes in the vas deferens. A decrease in protein in the vas deferens, seminal vesicle and prostate occurred. Inhibition of phosphorylase in vas deferens together with accumulation of glycogen altered its carbohydrate metabolism. We reported that the effects of sodium fluoride (NaF) ingestion in two doses (10 and 20 mg/kg body weight) for 30 days on histology and histocytometry of reproductive organs of the adult male mouse were investigated. The vas deferens epithelium showed nuclear pyknosis, clumped stereocilia, and cell debris but no sperm in the lumen and an increase in the lamina propria. Less literature is available on effect of sodium fluoride on vas deferens with short duration of albino rat. The purpose of the present work is to examine the effect of sodium fluoride on vas deferens of albino rats the following objective was studies. ∙ Body weight responses after treatment. ∙ Vas deferens weight change pre and post treatment. ∙ Histopathological alteration in the vas deferens in low dose and short duration

Keywords:- Male rats; Vas Deferens; Sodium Fluoride;

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E-18

Biochemical Studies Of Cestode Parasite Raillietina Fuhrmann of Gallus Gallus Domesticusfrom Georai

A. M. Budrukkar

Department of Zoology, R. B. Attal College, Georai

ABSTRACT

This paper contains biochemical study of cestode genus Raillietina of Gallus GallusDomesticus to understand their host parasite relationship. The protein contents in cestodes were estimated by the method given by Gornell et al. (1994) and lipid content were estimated by the Barner`s and Blackstock method (1973).

Protein estimation: The intestine of Gallus domesticus were examined at laboratory. The identical parasites were sorted out with the help of microscope. Small pieces of infected host intestine were also collected for the protein estimation. The protein content in the cestode parasites was estimated by Brand (1966) and Gornell et al. (1994) method. The worm were dried on blotting paper to remove water and taken wet weight of the tissue. The material was transferred in to previously weighted watch glass and kept in oven at 60°C for 24 hrs. Dried material was made into powder form. This powder weighed 250 mgs on balance. This material was grind with the help of mortar pestle. Added with 5 ml of 10% TCA. Material was transferred to test tube and centrifuged for10 min. at 2000 rpm. Discard the supernatant and taken the residue add 1 ml of distilled water and 3 ml of Biuret solution. The tube was kept for half hour until lavender colour is developed. Colour reading was noted on colorimeter at 530 mm to note Optical density

Keywords: Raillietina, Cestode Parasite, Gallus Gallus domesticus

Department of Botany, Deogiri College, Aurangabad. Page 52

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E-19

Prevalence of Bacterial Pathogens in Vegetables Commonly used as Salads

Sayed Rizwan A and Bagmar Chitra

Department of Microbiology,

Sir Sayyed College of Arts, Commerce and Science,

Aurangabad-431 001, Maharashtra, India. ------

ABSTRACT

Microbiological quality assurance is very important in food and hotel industries to prevent foodborne diseases. The present research work, therefore, was undertaken to assess the total viable count and bacteriological quality of salad vegetables, collected from several places of Aurangabad, Maharashtra, India. A total of 200 vegetable salad samples were collected and examined for enteric pathogens grown on EMB (Eosin Methylene Blue), S- S (Salmonella- Shigella) and MacConkey’s agars and found that 60 vegetables (30.0%) were culture positive. The food pathogens, were identified by the conventional methods. Incidence of Shigella was found maximum (10.5%) followed by Salmonella sp.(10.0%) whereas the E.coli noted less incidence (9.5%). Among all the collected salad vegetables, carrot was the highest contaminated.

Keywords: Foodborne diseases, vegetables, Shigella, Salmonella, E.coli.

Department of Botany, Deogiri College, Aurangabad. Page 53

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E-20

AVIAN POLYSPECIFIC CLUTCH SIZE AS BIOINDICATORS OF ECOLOGICAL CONDITION OF THE AREA

Chandrakant V. SIRSAT Department of Zoology, Vinayakrao Patil Mahavidyalaya, Vaijapur – 423 701,Dist. Aurangabad (MS) India

ABSTRACT

The present study deals with general trends in clutch-size of local birds and examined some of the hypotheses regarding the adaptive significance of the timing of breeding and variations in the clutch size, that have been put forth as possible explanations with in seasonal variation of average clutch-size of the birds. In the present work, recorded clutch size of 15 bird species belong to 10 families of 5 different orders most of them were passerines. Studies on clutch size helped to study life history traits of various birds, particularly Brahminy Myna Sturnus pagodarum and Indian Shikra Accipiter badius. Whereas clutch sizes of other birds provided an approximate measure about birth rate of that species. Clutch of each bird was observed approximately constant for that species. Further, this available information might be beneficial in future for detail study of that particular bird species from the local area. This study was carried out from July 2016 to June 2018 and simultaneously confirmed thereafter till June 2019.

Keywords:Clutch size, coefficient of variation, hatchling, nidicolous, atricial, precocious.

Department of Botany, Deogiri College, Aurangabad. Page 54

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E-21

Study on hygienic condition in Fish Market of Paithan Dist.Aurangabad Maharastra. D. R. Deshmukh and L.B.Dama

Department of Zoology, Pratishthan Mahavidyalaya, Paithan. M.S., India. Department of Zoology, Dayanand college,Solapur. M.S., India

ABSTRACT Now a days the fish and fishery products have become the most traded food items at National and International level. For effective controls and inspections in the fisheries marketing chain are of high importance for food safety and consumer protection. Many countries follows the regulations and control measures for good practices and handling and manufacturing for hygiene and health legislation. This work highlights on the hygiene requirements, controls and inspection of fresh water fish market for Safety, quality and production for the importance in the fishery industry. The safety and quality of fish depends on which condition the fish which are capture or harvest, and then how it is handled and stored before reaching to the consumer.The study was carried out to observed the infrastructural adequate facilities and adoption level of hygienic practices carried out by the fish marketing in Paithan. The Socio-economic condition of fisherman person involved in fish marketing activities were studied. The study revealed that availability index for the infrastructural facilities was 79.77%, and among various infrastructural facilities, icing (70.28%) and waste disposal facilities (71.66%) were reported. The overall adoption index of hygienic practices was 82.6%. The adoption of good hygienic practices was determined in maintaining personal cleanliness (90.00%), sorting of fish hygienically (85.28%), using clean container and polythene sheet (79.22%), method of waste disposal (78.22%), use of clean water for washing (95.55%) and use of ice to prevent fish spoilage (79.58%).

Keywords: hygienic condition, Fish Market, Paithan

Department of Botany, Deogiri College, Aurangabad. Page 55

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E-22

“Nematicidal activity of some common plant extract on population density of soil Nematode.”

Shaikh Unaiza Nazneen, J.D.Shaikh and Shaikh Tarranum Taneer

Department of Zoology Maulana Azad College of Science, Arts and Commerce, Aurangabad.

ABSTRACT

Present work conducted to evaluate the nematicidal activity of different common plant extract at different concentration .However these plant extract suppress the population density of nematode .The highest mortality shows by Neem leaf extact where as sesame seed extract gives lowest mortality rate. Research done on agricultural soil from Ajanta (Aurangabad ,Maharashtra) in laboratory condition by taking soil sample as controlled and experimental by arranging the set for 48 hours .The common plants selected for this experiment as they are widely available. The Neem leaves (Azadirachta indica),mint leaves(Mentha arvensis), coriander leaves(Condirum sativum) and ginger (Allum sativum)were selected at the concentration of 20%,40% 50%.

Keywords;Allum sativum, Condirum sativum, (Mentha arvensis

Department of Botany, Deogiri College, Aurangabad. Page 56

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E-23

Isolation of Lactic Acid Bacteria [PROBITICS] from homemade fermented food sample for Yoghurt formation.

Manisha Nitin Maind

Department of Microbiology,Deogiricollege,Aurangabad

ABSTRACT

An investigation of isolation of Lactic acid bacteria was carried out under low temperature for the preparation of yoghurt by using starter culture isolated. Lactic acid bacteria play a key role in the development of the organoleptic and textural qualities of fermented products.The aim of the recent study was the preparation of yoghurt [by using L.bulgarious&S.thermophilous] and improving the texture of the food. Lactic acid bacteria [LAB] played an important role in food technology. The lactic acid bacteria are gram positive microorganisms that play an essential role in the industrial production.Their enzymatic activities and the metabolic products they generate during the fermentation.The macromolecules play an important role in the development of the texture of yoghurt and fermented milk. Lab including strains of S.thermophilus ,L.lactis and a number of dairy lactobacilli.The total yield depends on the composition of the growth medium,the temperature,pHand the incubation time. Lactic acid bacteria [LAB] dominated the microbial population of yoghurt and were identified accourding to their morphological and physiological characteristics.Among these lactobacilli were frequently occurring organisms.The most abundant species were lactobacillus delbrueckii subspp.Bulgaricus and Streptococcus thermophilus. The Lactic streptococci was subjected to biochemical tests to identifiey the species.Based on the biochemical reactions the specieswas identified as Lactococcuslactis, subspp. di-actylactis. Isolated culture of lactic streptococci was found to grow at low temperature.When this was used as inoculum to prepare yoghurt at 5 o C,15 o C and 22 o C curdling took place in 3 days time.In order to reduce the settting time, nutrients in the form of chopped fruits added. The yoghurt was found to set at 5 o C in 30 hrs which is considered useful..Acidity of yoghurt was found to be 0.53%-0.55%. The yoghurt was found to contain di-acetyl and quality of yoghurt was good. S. thermophilus is LAB widely used in the manufacturing of dairy products in fact it is the most important thermophilic dairy starter.

Department of Botany, Deogiri College, Aurangabad. Page 57

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The species is able to grow at high temperture [45 o C] required dairy products manufacturing processes..For yoghurt fermentation S.thermophilus is used alone or in the combination with different lactobacillus and mesophilicstarters,while for yoghurt it is normally use with L.delbrueckiisubspp.bulgaricus. The role of S.thermophilus in the milk fermentation is the rapid conversion of lactose into lactic acid, which causes a rapid reduction in the pH and encourages the production of metabolites important to the final flavor.A number of S.thermophilus.Which contribute to the desirable viscous texture & rheological properties of fermented milk products, particularly yoghurt.The interest of industry in the finding starters with much attention is being paid to improving thetexture of food .Lactic new metabolic properties has revived the idea of screening artisanal dairy products of different origin.Currently acid and probiotic bacteria were enumerated and isolated from homemade preparations and probitic milk products. Lactobacillus delbrueckii spp. Bulgaricus were enumerated and isolated using MRS agar used for theenumeration and isolation of probiotic bacteria which was incubated anaerobically at 37 o C for 72hrs. Morphological, physiological and biochemical reactions used to characterize the isolates.

Keywords:-Yoghurt,Lactic Acid Bactria, S. thermophilus,Isolation,Identification.PROBIOTC,Fermented homemadeprepartions, Health benefit.

Department of Botany, Deogiri College, Aurangabad. Page 58

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E-24

ESTIMATION OF PROTEIN CONTENT IN FISH MUSCLES FROM THE LOCAL FISH MARKET, NANDED CITY

M. S. Kadam Department of Zoology, Yeshwant Mahavidyalaya, Nanded - 431602, Maharashtra

ABSTRACT Fishes play an important role in human nutrition in India, particularly people of Coastal areas. Good and adequate nutrition plays important role in expression of mental, physical and intellectual qualities in humans. To ensure access to nutritionally adequate food for the improvement in the quality of diet of a poor person in the society, fish is the only medium which can serve the very purpose. They have the ability to reduce blood lipid level, particularly serum triglycerides. Fishes are good source for human nutrition due to their therapeutic role in reducing certain cardio vascular disorders. Fish is the constituting the only animal protein source among rural poor households. Fish is a rich source of proteins, carbohydrates, minerals and vitamins etc. Proteins are the most important characteristics of living matter and are also the principal constituents of protoplasm which from the material basis of life (Jain and Singh, 1981). The locally available freshwater fishes such as Notopterus notopterus, Cyprinus carpio and Mystus seenghala were selected for the present study for estimation of total proteins from Nanded region. The estimation of protein was carried out by Lowry’s Method in muscle tissue. The comparative data collected to examine the richness of proteins in fish muscles. The obtained data showed that the amount of proteins in fish muscles is higher in Notopterus notopterus as compared to Cyprinus carpio and Mystus seenghala.

Keywords:Notopterus notopterus, Cyprinus carpio, Mystus seenghala, Total Proteins, Muscles.

Department of Botany, Deogiri College, Aurangabad. Page 59

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E-25

COMPARATIVE STUDY OF WINGS OF BUTTERFLY GRAPHIUM AGAMEMNON AND MOTH THYAS CORONATABY SCANNING ELECTRON MICROSCOPY

H. P. Nandurkar

Deptt. Of Zoology, Sant Gadge Baba Amravati University, Amravati, M.S., India. 444602

ABSTRACT

Butterflies and moths have different wings than those of other insects. They are formed from a transparent double epithelial chitin membrane which is crisscrossed with tubular veins. Scales of Butterfly and moth are present on the wings which are very tiny and arranged in a specific manner. The scales may help to attract a mate, or keep the predators away due to interference of light into the UV range and also are used as communication signals for attraction, repulsion, or camouflage. The morphology of scales can be studied using Scanning electron microscopy for the identification of butterfly and moth scales. A butterfly Graphium agamemnon belonging to familyPapilionidae and a moth Thyas coronataof the family Noctuidae are selected for comparative study in present work. This butterfly species has strikingly iridescent colours with green range; this colour formation is by the micro-structuring of the wing scales. The present study of Scanning Electron Microscopic structure of wing scales of the butterfly & moth species was carried out in the Department of Metallurgy, Visvesvaraya National Institute of Technology, Nagpur (M.S) India. Scanning electron microscopy revealed that there are 3 to 4 ridges present on the terminal portion of the G. agamemnon wings in comparison with a single ridge in given moth. Length and width of the scale of butterfly is 130 μm and 42.5 μm and that of T.coronata is 108 μm and 31.1 μm respectively. The pattern between two trabaculae has two rows of pentagonal or polygonal holes in G. agamemnon, on the other hand the pattern issquare form in T.coronata. All these unique characteristic also provide a tool for identification of species of butterflies and moths.

Department of Botany, Deogiri College, Aurangabad. Page 60

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E-26 Kinetics of Renal Alkaline Phosphatase of catfish, Clarias gariepinus

S.B. Patharde* and A.M.Chilke** *Centre for Higher Learning and Research, Department of Zoology, Sardar Patel College, Chandrapur-442402 **Division of Toxicology and Biomonitoring, Department of Zoology, Shree Shivaji Arts, Commerce and Science College, Rajura-442905 (M.S.)

ABSTRACT Kidney is an excretory center of the vertebrate body and it carries out excretory function. Alkaline phosphatase is widely studied enzyme and tested for diagnosing the malfunctioning of tissues or organs. The effect of pH, temperature, time and substrate concentration was studied by exposing the enzyme to the range of pH from 8.0 to 11.5, temperature ranging from 300C to 600C, time ranging from 10 to 80 min, and substrate ranging from 0.8 mM to 4.0 mM, however the range of enzyme concentration from 20 µl to 120 µl was used for studying the effect of enzyme concentration on reaction velocity. As negligible work has been done on the kinetics of enzymes in fishes in general and therefore, we aimed to study to find the kinetic properties of this enzyme in the kidney of Clarias gariepinus. Renal alkaline phosphatase exhibited maximum activity at temperature 40°C and pH 9.5 and these are considered to be the optima for this enzyme. However, the enzyme activity was observed to increase consistently with increase in time and enzyme concentrations. Similarly, increase in the concentration of substrate to certain extent found to stabilizes the enzyme activity and it was considered as Vmax. The Vmax and Km values were found to be 196.07 µg/gm wt. of kidney and 5.15 mM from resulted graph.

Department of Botany, Deogiri College, Aurangabad. Page 61

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E-27

“Studies on biology of fruit piercing moth Othreismaterna (Noctuoidea: Lepidoptera) from Marathwada (M.S.) India”

Shendge E.S, Kayande H.G, Chavan R.J.

Department of Zoology, Dr.Babasaheb Ambedkar Marathwada University Aurangabad, Maharashtra, India

ABSTRACT

The present communication deals with study of biology of fruit piercing moth Othreis materna on pomegranate from Marathwada. During the study morphology and behavior of all the stages of life cycle were recorded in laboratory condition. The present study shows that there is occurrence of fruit piercing moths of Genus Othreis, as a harmful pest on pomegranate and other fruit crops in different parts Marathwada. The fruit piercing moths Othreis attacks on various fruits crops but they mostly found on pomegranate, citrus and orange in Maharashtra state, India.

Keywords: Fruit piercing moth, Pomegranate, Biology, Othreis materna (L).

Department of Botany, Deogiri College, Aurangabad. Page 62

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E-28

A COMPARATIVE STUDY OF AZOTOBACTER SPP. FROM VARIOUS LOCALITIES OF BEED DISTRICT (M.S.)

M.T. Kuchekar and B.T. Pawar

Research Center in Botany,Shri Muktanand College, Gangapur – 431109 Dist. Aurangabad

ABSTRACT

Present investigation was carried out to study Azotobacter spp. Collected from Rhizosphere soil samples of various crops from Beed district (M.S.). Twenty two Rhizosphere soil samples were collected from various localities of the district and soil testing was done to determine soil pH, water holding capacity and moisture content. After isolation of the bacteria from Rhizosphere, biochemical tests were performed to study their characters. Bacterial strains collected from various locations were named as Strain 1 to Strain 22. Out of these strains 3, 7, 9, 11, 19 and 21 did not show characters identical with Azotobacter; while other strains resembled Azotobacter spp. Cell size of Azotobacter ranged in between 1.05 to 2.03 μm. Colonies were creamy white, circular, small to large sized, opaque with smooth margins. Biochemical tests confirmed responses to citrate, starch hydrolysis, catalase, indole production, urease and nitrate reduction. Azotobacter is widely used as bio-inoculants for wide variety of crops, and hence, it’s morphological and biochemical study of Azotobacter spp.will be useful for further research.

Keywords: Azotobacter spp., Biochemical test, Beed district, Rhizosphere.

Department of Botany, Deogiri College, Aurangabad. Page 63

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E-29

Living Habitat of Freshwater Fish Rasbora daniconus in Bindusara Basin, Beed. * Sayad Jalil Hussain, *Mohammad Ilyas, *Shaikh Feroz. * Department of Zoology. Milliya Arts Science and Management Science College, Beed.

ABSTRACT

In the present communication habitat ecology, species diversity; distribution and different indices of fish biodiversity management were studied in the Bindusara Basin, Correlation between fish species richness with the hydrological attributes showed good relationship and water depth, dissolved oxygen and pH were found the most important variables in shaping fish assemblage.

Keyword: Bindusara River, Habitat Ecology, Rasbora Daniconus. Trophic niche.

Department of Botany, Deogiri College, Aurangabad. Page 64

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E-30

Carotenoid: As a bioactive compound

Deepti D. Dhere1 and S. M. Dharmadhikari2

1 Deogiri College, Aurangabad 2Government College of Arts and Science, Aurangabad

ABSTRACT Carotenoids are terpenoid pigment with polyene nature. They are produced by plants, fungi, actinomycetes and bacteria as a protective molecule. Carotenoids are yellow, orange and red pigmentsss. These compounds are known for their provitamin A activity and antioxidant function. Due to these functions they can be used to prevent many diseases caused by free radical damage e.g. hypertension, atherosclerosis, Alzimer’s, Parkinson’s, cataract, cancer, etc. Their regular consumption can slower the process of aging. In recent work carotenoid was extracted from Paracoccus beibuensis SL2 a halo-alkalotolerent bacterial species and its activity was studied as an antioxidant by DPPH assay (1,1 diphenyl 2-picrylhydrazyl) and as a fish food supplement. The carotenoid extract showed 56% antioxidant activity and IC50 at 4.6 μg/ml. Supplementation of carotenoid improved skin pigmentation and other health parameters in gold fish Carassius auratus. The research concludes that bacterial carotenoids can be used as potential bioactive compound.

Keywords- Bioactive compounds, Carotenoids, antioxidants, DPPH.

Department of Botany, Deogiri College, Aurangabad. Page 65

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E-31

Checklist of Wasps, Bees and Bumble Bees (Insecta: Hymenoptera) from K.T.H.M. College Campus, Nashik. Gurule Sachin Arjun*, Gangurde Jyoti Haribhau and Jadhav Tushar Ananda MVP’s K.R.T. Arts, B.H. Commerce and A.M. Science (KTHM) College, Nashik-02.

ABSTRACT Diversity of wasp, bees and bumble bees is explored from KTHM College campus, nashik during the July 2015 to February 2016. The checklist of wasp, bee and bumble bees prepared by collecting dead specimens found within the KTHM College campus. In the present study 25 species belongs 21 genus and 11 families of Hymenoptera has been recorded. The identification of collected specimen was confirmed by comparing the specimens available in collection department of Bombay Natural History Society (BNHS), . Family apidae found to be dominating family represented by four species Apis dorsata, A. cerena indica, Thyrus ramosus and Amegila cingulate while families Vespidae. Xylocopidae, Eumenidae and Sphecidae represented by 3 species each. Families Chrysididae, Ichneumonidae and Braconidae were represented by single Stibum cynarum, Coelichmeumon haemmorhoidalis and Cremnops desertor respectively. Of the collected specimens eight species remains unidentified due to scanty literature and efforts to identify.

Keywords: Hymenoptera, Diversity, Bees, Wasps, Bumble bees, Nashik, KTHM College.

Department of Botany, Deogiri College, Aurangabad. Page 66

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E-32

COMPARATIVE STUDY OF WINGS OF BUTTERFLY GRAPHIUM AGAMEMNON AND MOTH THYAS CORONATABY SCANNING ELECTRON MICROSCOPY

H. P. Nandurkar

Deptt. Of Zoology, Sant Gadge Baba Amravati University, Amravati, M.S., India. 444602

ABSTRACT

Butterflies and moths have different wings than those of other insects. They are formed from a transparent double epithelial chitin membrane which is crisscrossed with tubular veins. Scales of Butterfly and moth are present on the wings which are very tiny and arranged in a specific manner. The scales may help to attract a mate, or keep the predators away due to interference of light into the UV range and also are used as communication signals for attraction, repulsion, or camouflage. The morphology of scales can be studied using Scanning electron microscopy for the identification of butterfly and moth scales.

A butterfly Graphium agamemnon belonging to familyPapilionidae and a moth Thyas coronataof the family Noctuidae are selected for comparative study in present work. This butterfly species has strikingly iridescent colours with green range; this colour formation is by the micro-structuring of the wing scales.

The present study of Scanning Electron Microscopic structure of wing scales of the butterfly & moth species was carried out in the Department of Metallurgy, Visvesvaraya National Institute of Technology, Nagpur (M.S) India.

Scanning electron microscopy revealed that there are 3 to 4 ridges present on the terminal portion of the G. agamemnon wings in comparison with a single ridge in given moth. Length and width of the scale of butterfly is 130 μm and 42.5 μm and that of T.coronata is 108 μm and 31.1 μm respectively. The pattern between two trabaculae has two rows of pentagonal or polygonal holes in G. agamemnon, on the other hand the pattern issquare form in T.coronata. All these unique characteristic also provide a tool for identification of species of butterflies and moths.

Department of Botany, Deogiri College, Aurangabad. Page 67

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E-33

Enrichment, isolation and screening of potential pectinase producing organism

Patidar Kavisha K., Dr.S. A. Peshwe

Government Institute of Science, Aurangabad, India

ABSTRACT Isolation of extracellular pectinolytic enzyme (polygalacturonase PG) producing microorganisms was done from 16 soil samples of Aurangabad using nutrient enrichment method. Preliminary screening of pectinase producing microorganism was done by spot inoculation on yeast extract pectin agar medium. Out of 50 isolates, 26 isolates were found to be positive for pectinase production on pectinase assay plates as evidenced by clear hydrolization halos. From these microorganisms, 20 isolates were able to synthesize PG with activities above 3 U mL−1. Out of 20 isolates, 12 were bacteria, 4 were fungus and 4 were actinomycetes. KP10B was found to be potent pectinase producer having maximum polygalacturonase activity (92 U mL−1).

Department of Botany, Deogiri College, Aurangabad. Page 68

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E-34

Evaluation of Antibacterial Activity of Leaf Extract of Jasminum Officinale Against Oral Pathogens in Ulcer Treatment

Mr. Sopan A. Shinde Dr. Vanita U. Pochhi

Shri Shivaji Science and Arts College Chikhli, Dist Buldana, 443201 (MH)

ABSTRACT

Medicinal Plants are endowed with phytochemicals that are vital to counter variousmetabolic disorders like Oxidative damage in cells causing various degenerative diseases. Hence,the present study deals with the assessment of antioxidant activity and phytochemical screening ofthe aqueous extract of Jasminum officinale leaves. From ancient times, plants have been used intraditional medicines for treatment of different ailments. Medicinal plants is one of the richest bioresources for traditional and folk medicines till date. Jasmine is botanically known as Jasminumofficinale or Jasmininie and belongs to the olive family of Oleaceae. Literature report suggest thatJasmine is analgesic, antidepressant, antiseptic, expectorant, aphrodisiac, sedative, stomachic,diuretic, depurative, astringent, stimulating, anti-oxidizing, anthelmintic and anti-inflammatory innature. The objective was to study antibacterial activity of Jasminum officinale extracts againstmouth ulcer causing organisms. The antibacterial activity has been studied against Escherichiacoli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis & Enterococcus faecalisby agar well diffusion method. Leaves extract of J. officinale give effective results against oralpathogens causing mouth ulcer. Acetone and Ethanol extracts displayed a good antibacterialactivity. The phytochemical studies revealed presence of Carbohydrates, Proteins, Steroids,Alkaloids, Flavonoids, Phenols, Saponins, Glycosides and Tannins. J. officinale may prove to beeffective medicine for the treatment of ulcer.

Department of Botany, Deogiri College, Aurangabad. Page 69

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E-35

“Preliminary investigation of Protozoan ciliates of Family Isotrichidae from the rumen of domestic goat”

SANGHAI P.K. *Head, Department of Zoology, Shivaji A.C. & Sci. College, Kannad -431103, Dist. Aurangabad (M.S.) India.

ABSTRACT

A survey has been made to study the ciliates found in the rumen of domestic goats Capra hircus. In the present investigation ciliates belongs to the family Isotrichidae were observed from the rumen of domestic goat. The family includes three genera of which Isotricha and Dasytricha were observed. The ciliates were identified and described at species level. The morphology, dimensions, variations in the structure have been recorded. The detail study will be carried out.Keywords: Rumen, Protozoa, Ciliates, Isotrichidae, Goat

Department of Botany, Deogiri College, Aurangabad. Page 70

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E-36

Biosurfactant: A New Alternative to Chemical Surfactant for Bioremediation Gadakh PV and Pawar AL Department of Microbiology, ASC College, Badnapur, Jalna 431202

ABSTRACT

Biosurfactant are valuable microbial amphiphilic molecules with effective surface active and biological properties applicable to several industries. Microbes synthesize them, especially during growth on water immiscible substrates, proving an alternative to chemically prepared conventional surfactants. In recent years, natural biosurfactants have attracted attention because of their low toxicity, biodegradability and ecological acceptability. Present work deals with the screening of biosurfactant producing ability of the isolates on different carbon source. The petroleum contaminated soil samples were collected from petrol pump and garages of Amravati city. Total fifteen samples were screened on MSM medium containing 2% oil as carbon source. From fifteen samples six different isolates were isolated. Out of these six isolates one isolate shows surface tension reduction upto 47.19dyne/cm and 32.24dyne/cm and zone of oil displacement upto 35mm and 80mm when motor oil and mustard oil used as carbon source respectively. All isolates were identified by 16S rRNA sequencing they belong to genus Pseudomonas aeruginosa, Aneurinibacillus miugulanus, Achromobacter insolitus, Bacillus shackletonii and Ochromobactrum intermedium. These isolates may be used as potential bacteria for remediation of the petroleum contaminated soils.

Keywords: surface active agent, A. migulanus, oil displacement test, surface tension

Department of Botany, Deogiri College, Aurangabad. Page 71

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E-37

Synthesis and Antimicrobial Study of Rare Earth Complexes of Schiff Base Derived from 4-Amino Benzoic Acid

Atish R. Mehetre1*, Sandeep R. Deshmukh2 and Vijay N. Bhosale3 1,3 Department of Chemistry, Shivaji Arts, Commerce and Science college Kannad, 431103(MS), INDIA. 2 Department of Chemistry,R.M.G. Arts, Commerce and Science College Saoli, 441225(MS), INDIA.

ABSTRACT

Schiff base was prepared by using 4-amino benzoic acid and ortho-vanillin, namely (E)-4-((2-hydroxy-3-methoxybenzylidene)amino)benzoic acid. Rare earth complexes were prepared by using nitrates of Cerium, Gadolinium and Dysprosium with Schiff base. Synthesized Schiff base and complexes were structurally characterized by UV, IR, NMR, HRMS, TGA and elemental analysis. Structural characterization shows 1:2 metal to ligand ratio in the complexes with the coordination of water molecule. The synthesized Schiff base and complexes were screened for their biological activity against bacterial species Gram –ve bacteria (Escherichia coli), Gram +ve bacteria (Staphylococcus aureus) and fungi (aspergillusniger).

Department of Botany, Deogiri College, Aurangabad. Page 72

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E-38

Changes in the glycogen content of freshwater bivalve Indonaia caeruleus (Prashad, 1918) with injections of cerebral ganglionic extract and equivalent commercial hormones (progesterone and estradiol) during winter season.

1Thorat S. K*, 2Vedpathak A.N.

1 Department of Zoology, Smt. G. G. Khadse College, Muktainagar, Jalgaon. (M.S.). 2, Molluscan Endocrinology & Physiology Laboratory, Department of Zoology, Dr.Babasaheb Ambedkar Marathwada University, Aurangabad-431004(M.S.).

ABSTRACT

We report here the effect of injections of cerebral ganglionic extract and equivalent commercial hormones (Progesterone & Estradiol) on glycogen metabolism of freshwater bivalve mollusc Indonaia caeruleus (Prashad, 1918) from Godavari River, as we know the importance of neuroendocrine control on the metabolic physiology of freshwater bivalves. During winter season, the adult bivalve mollusc, Indonaiacaeruleus (50-55 mm shell length) were subjected to the five respective experimental groups are as follows- 1) injection of commercial hormone progesterone 2) injection of ganglionic extract 3) injection of sham operation 4) injection of estradiol and 5) control (normal) for 10 days. The glycogen estimation in bivalves from all four groups (including control) was measured on 3rd, 6th, and 9th day. The study revealed that, the glycogen content was significantly decreased from mantle and foot in all experimental groups, as well as the content increased significantly from gonadal tissue on 3rd day. During 6th day, the glycogen content increased significantly from mantle and hepatopancreas in all experimental groups. On 9th day, the glycogen content decreased significantly from hepatopancreas in progesterone and ganglionic extract injected group; whereas the content increased significantly from mantle in progesterone injected and from foot in ganglionic extract injected group.

Keywords: - Cerebral ganglionic extract, Progesterone, Estradiol, Glycogen estimation, Freshwater bivalve.

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Comparison of Phospholipase A2 from Honeybee Venom by In Silico Method

Vijayshree Hemke Department of Zoology Shri Shivaji Science And Arts College, Chikhli. Dist: Buldana

ABSTRACT

Honeybees play a significant role in each aspect of the ecosystem. Besides other products, honeybees can produce venom for their protection. Phospholipase A2 enzymes are commonly found in insect venom. The venom of honeybee contains different peptide chains. PLA2 enzyme is one of the major content of insect and snake venom. Here we study PLA2 of venom of Apis dorsata, Apis Mellifera, Apis cerena, Apis indica for its antigenic determinants by in silico method. As our knowledge of the immune responses to a protein antigen progressed, it is now clear that the whole protein is not required for raising the immune response, but small segments (“DTKCYKLEHPVTGC” 92-105 aa of Apis dorsata) of a protein called the antigenic determinants or the epitopes are sufficient for eliciting the desired immune response. The immune response is triggered through B-cells and T-cells, at a cellular level, epitopes play a very important role to stimulate immune response. If antigens are in a tertiary structure T-cells can not recognize it, but rather as fragments of peptide, so antigenic determinant is important. Melittin is a major component of honeybee venom, which is a stimulant of Phospholipase A2. Arachidonic acid is released from the phospholipid membrane disproportionately resulting from a hymenopterans’ sting due to the increased presence and activity of PLA2. It results, inflammation and pain occur at the site of the sting. Now a day’s honeybee venom is used for treating a wide variety of medical conditions like rheumatoid arthritis by identifying its epitopes.

Keywords: Venom, In Silico prediction, PLA2, Antigenic Determinants, Apis

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Development and Characterization of Bacterial Consortium for Bioremoval of Cr (VI). Sakale S. S*. &Chitanand M. P**. Department of Microbiology, N.S.B. College, Nanded-431601, Maharashtra, India.

ABSTRACT

Waste water of industries like electroplating, metal alloys, leather, tanning, contains hexavalent chromium which represents a well recognized bio-hazard. Cr (VI) is one of the major reasons for the environmental pollution and has become the attention of great concern in the world due to the extensive release of such toxic metal in an environment including soil and water. Recently remediation of Cr (VI) using bacterial consortium has gained importance, as it results in processes with clean technologies producing maximum benefits, due to its adaptability and environment-friendly nature.

In the present study, it was aimed to develop and study of efficient bacterial consortia for reduction of chromium metal.Nineteen Cr resistant isolates were isolated from electroplating effluent samples, out of which five isolates showed higher MIC for Cr (VI) 1300µg/ml. Five isolates were identified as C. amylolyticum, B. cereus,S. arlettae, A. pascensand C. funkei by 16s r-RNA gene sequencing. By using these five isolates, three bacterial consortia G, J, and M were developed. These three consortia showed higher Cr reduction efficiencies than five individual isolates. The comparative study of three consortia represents that, Consortium M showed the highest Cr reduction efficiency than Consortia G and J. Results of further characterization of consortium M showed that, maximum Cr (VI) reduction could be observed at pH 7 and temperature 30ᵒC. At 10µg/ml concentration of Cr (VI), consortium M could reduce 100% of Cr (VI), in 6hrs of incubation period. But as initial concentration of Cr (VI) was increased 20, 50 and 100µg/ml, Cr reduction ability was decreased up to 83.45%, 61.11% and 40.33% respectively. In presence of Cu and Ni, Cr (VI) reduction efficiency of Consortium M was increased and 100% reduction of 10 µg/ml Cr (VI)

Department of Botany, Deogiri College, Aurangabad. Page 75

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The results confirmed that the consortium developed in the present work rather than individual isolates is an effective tool of bioremediation for removal of toxic Cr (VI) from chromium contaminated sites.

Keywords: Electroplating effluent, Metal Bioremediation, Diphenyl Carbazide Method (DPC).

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Morphological Studies of Chilodonella cyprini (Moroff 1902) from freshwater fishes

T.T.SHAIKH Department of Zoology, Maulana Azad college of arts Science & Commerce, Aurangabad

ABSTRACT

In the present study morphology of Chilodonella cyprinifrom fresh water fishes have been described,occurring in particularly on the gills and in a later stage on its skin. The relative prevalence of this protozoan parasite among different fresh water fishes from Aurangabad are analysed. Over all percentage of prevalence for the whole year was 46.60 %. This parasite showed highest prevalence in the months of November to February, moderate in July to October whereas minimum prevalence was recorded in the March to June.

Key words: Chilodonella cyprinid, gills, fishes, prevalence

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ANALYSIS OF PHYSICO CHEMICAL PARAMETER OF SOIL SAMPLES AND NEMATODE DIVERSITY OF SEASONAL CROPS FROM SAKUR REGION, TAL. SANGAMNER (MS) INDIA.

K. D. Thete1*, L. V. Shinde2, S. B. Bhadange1 and R. B. Antre1 1. Department of Zoology, Padmashri Vikhe Patil Arts, Science and Commerce College, Pravaranagar – 413713, Tal. Rahata, Dist. Ahmednagar 2. Department of Zoology, J. E. S. College, Jalna (MS) India

ABSTRACT

In the present study, we analyze the physico-chemical parameters of soil samples from different localities of seasonal crops from Sakur region. The soil samples were collected and analyzed to measure various physical and chemical parameters by standard methods. The soil parameters like pH, temperature, TDS, EC, organic carbon, alkalinity, calcium, magnesium, nitrogen, potassium and phosphorus were studied. From the analysis, the variation in physico-chemical parameters observed with respect to crop field pattern. In continuation to our research, study was carried out for nematode diversity of seasonal crops. Nematode diversity shows their interactions with plants and other organisms. They play important role in nutrient cycle and as plant parasites. The recorded nematode species are Meloidogyne incognita, M. javanica, Rotylenchulus reniformis and Heterodera glycines. From these, most dominant species are M. incognita, M. javanica which found in all seasonal crops.

Keywords: Soil, seasonal crop, physico-chemical parameter, Nematodes, Sakur region.

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Study the Effect of pH and Temperature on Protease Production by Psychrotrophic Pseudomonas Species Isolated from Dairies of Aurangabad.

Syed Nisar Ahmed*, Mohammad Shakir

Department of Microbiology, Sir Sayyed College, Aurangabad; Department of Microbiology KSK College, Beed.

ABSTRACT

Psychrotrophic bacteria are able to grow at 70C or lower but had higher growth temperature called psychrotrophs. They are commonly found in different cold environments such as deep oceans, Antarctic regions, seasonally cold environments as their potential ability to sustain high temperature. Psychrotrophs produce cold active enzymes which reveals higher catalytic activities as compare to mesic and thermic conditions. Most psychrotrophs produce hydrolytic heat stable extracellular enzymes like proteases, lipases, phospholipases etc. in milk or dairy products stored at low temperature. In this present study cooled buffalo milk was used for the isolation of psychrotrophic pseudomonas species and its protease production was studies on different pH and temperature. On skim milk agar a zone of hydrolysis showed proteolytic activity of Pseudomonas and it is measured by selection ratio. Selection ratio (SR) = diameter of zone of hydrolysis / diameter of colony in mm. proteolytic activity was optimized on different pH and temperature. Results showed that maximum protease production was observed at 370C and pH 7.0 which is 6.0 ± 0.1 SR value. Psychrotrophic bacteria also able to produce protease at refrigeration temperature and on prolonged cooling can alter the quality of milk.

Keywords: Psychrotroph, protease, selection ratio, zone of hydrolysis.

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“ULTRASTRUCTURE OF THE SKIN OF THE TREE FROG”

Polypedates leucomystax

I.R. USTAD

Dept. of Zoology

Sir Sayyed College, Aurangabad.

ABSTRACT

Amphibians live in varied habitats. This fact probably explains the great morphofunctional diversity in their skins Although the integument is the structural and functional interface between the organism and its environment, the morphological and complexity of amphibian skin is not fully understand. There is practically no information on the integument of the tree frog Polypedates leucomystax found in cherrapunjee. Therefore, ultra structure of the skin of the adult frog of Polypedates leucomystax was studied during the breeding phase with the help of transmission electron microscopy. Adult males and females of Polypedated leucomystax were collected from the study sites at Cherrapunjee. The epidermis consisted of four layers namely, stratum cvornum, stratum granulosum, stratum spinosum and stratum germinativum. Desmosomes, ribosomes, tonofilaments. Golgi bodies, vacuoles and collagen fibres were observed in the epidermal cells. The basement membrane composed of collagenous fibres separated the epidermis from the dermis. Pigment cells like pterinosomes, melanophores and iridiphores were abserved and these pigment cells may work together to determine the varied skin colouration and patterns displayed in polypedutes leucomystax and my have important roles in adapting this frog to its environment.

Keyworeds: polypedates leucomystax; epidermis; pigment cells.

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“STUDIES ON DIVERSITY OF MOSQUITO LARVAE IN AURANGABAD CITY, MAHARASHTRA, INDIA’’ S.R. Sule, R.J.Chavan, L.V.Shinde, H.G Kayande.

Department of zoology, Dr.Babasaheb Ambedkar Marathwada University Aurangabad and J.E.S College, Jalna Maharashtra,India.

ABSTRACT

Mosquito are tiny insects of order Diptera. All over world India ranks 5th for mosquito diversity. Mosquitoes act as a vector for several diseases. The study of mosquito diversity is the essential to find out new control strategies. The present study deals with the diversity of mosquito larva in Aurangabad city of Maharashtra, India. The mosquito larva was collected from 10 sampling sites and rearing was carried out in the laboratory. Total four species of genus Aedes, Anapheline, Culex such as species Aedes aegypti, Aedes albopictus, Culex quenquifasciatus, Anopheles Culicifacies were identified as per the key and description given by Christoper (1933), Nagpal and Tingare (2010), Sathe and Girhe (2002). The study reveals that in month September and October there is high abundance of Aedes agypti and Aedes albopictus. As they are key vector of dengue diseases during this period dengue patients number is increased in Aurangabad city in November month Culex species number is high.

Keywords: Larval, diversity, epidemics, Aedes aegypti, Aedes albopictus, Anaphelines. kiu

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PREVALENCE OF ECTOPARASIDES INFESTATION ON MILCH CATTLS FROM AURANGABAD DISTRICT, AURANGABAD, (M. S) INDIA.

Sushama Paikade, Ramrao Chavan, Hanumant Kayande

Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad.431004 (M.S) India

ABSTRACT A study was conducted during June 2015 to May 2016 for the prevalence of ectoparasites infestation on milch cattles from Aurangabad District M. S. India. Total 544 cattles were examined which includes 360 cow and 184 buffaloes. The prevalence of ticks was higher in cow (58.33%) as compared to buffaloes (45.10%), the lice prevalence was higher in buffaloes (36.41%) as compared to cow (22.22%), and the flies prevalence was higher in buffaloes (52.17%) as compared to cow (33.61%). The prevalence showed that the milch cattles were infested by single or mixed ectoparasites. Thus, the present investigations suggest that there is need of creating awareness among farmers about to vaccination hygienic precautions in milch livestock.

Keywords: Prevalence, ticks, lice, fly, livestock, Aurangabad.

Department of Botany, Deogiri College, Aurangabad. Page 82

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Analysis of Enzyme Activity during Vermicomposting of Organic Wastes (corn cob and cauliflower)employing the earthworm Eudrilus eugeniae

Vishal Bedre1 and Balraj Khobragade* PG Department of Zoology, Ahmednagar College, Ahmednagar – 414 001 Maharashtra (India)

ABSTRACT

Earthworms consume dead and decaying organic matter along with soil. Enzymes in their digestive tracts carry out biodegradation of this partially decomposed content. The egested excreta called ‘vermicast’ contain plant growth hormones, various organic and nitrogenous compounds. The resultant humus-like material is called ‘vermicompost’, which enhances soil fertility and eventually promotes increase in plant production. A greatly populous nation like India faces the overwhelming challenge of waste disposal. Further, there is inefficient disposal of organic wastes, which actually have the potential to be converted into cheap and easily available biofertiliser. This natural fertiliser may be employed for augmenting output of economically important crops. The quality of vermicompost depends on the substrate acted upon by enzymes in the earthworm gut. The extent of enzyme activity varies as per the substrate and the stage of vermicomposting operation. Keeping this in view, the present study was undertaken to analyse the enzyme activity during various stages of vermicomposting of the corn cob and cauliflower waste using the earthworm species Eudrilus eugeniaecommonly popularly known as ‘African Night Crawler’. The relation between the type of substrate and enzyme activity in the gut of the earthworm during the composting process was investigated, which revealed that corn cob showed good enzyme activity as compared to cauliflower waste. Enzymatic analysis provided evidence of presence of the enzymes namely amylase, invertase and urease in the gut of Eudrilus eugeniae. Quantitative analysis of the enzymes recorded maximum amylase and invertase activity to be 11.2 and 9.6 µg/ml/min and 8.33 and 6.46 µg/ml/min at 30th day interval; whereas, maximum ureaseactivity was found to be 3.8 and 6.1 µg/ml/min at 45th day interval; in the gut content of Eudrilus eugeniae fed on corn cob and cauliflower waste, respectively. Enzymatic analysis of earthworm gut content suggests that corn cob waste and cauliflower waste can be used as good organic substrate by the earthworm species Eudrilus eugeniae. It can be concluded that ‘vermitechnology’ is an efficient and secure substitute tool having tremendous prospective in organic waste management.

Keywords: Vermicomposting, organic wastes, earthworm gut enzymes, Eudrilus eugeniae

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Antibiotic Resistance Pattern among Fecal Indicator Bacteria in Hospital Waste Water

R. N. Jadhav Shivneri Mahavidyalaya, Shirur(A). (MS), India.

ABSTRACT

No life can exist without water. There are thousands of microorganisms which live in water & are transported through it. Water receives microorganisms from air, sewage, soil, organic wastes, dead plants, animals etc. Hospital waste water can be a potential risk factor for public health & ecological balance, as it contains various hazardous components including pathogenic microorganisms E. coli is an indicator of fecal pollution of water. It is a member of coli form group of bacteria. It is an example of opportunistic pathogen. It is a normal flora of intestine of warm blooded animals. Diarrhea, urinary tract infection & nosocomial infection are caused by E. coli. Drug resistance in pathogenic bacteria has been commonly reported throughout the world. The resistance of bacterial pathogens to many antibiotics continues to increase globally. By different ways bacteria become resistant to antibiotics. Patients suffering from drug resistant strain fail to respond drugs treatment. So there is a continuous demand of new drug. In the present study isolates of E. coli were isolated from hospital waste water in Latur city. Isolate of E. coli were identified according to their morphological, cultural characteristics and biochemical tests. Antibiotic susceptibility test of the clinical isolates was done by using modified Kirby-Bauer disc diffusion method according to the guidelines of the CLSI. Interpretation of resistance was based on the NCCLS criteria. The most common pattern of multiple drug resistance of isolates of E. coliobserved was ampicillin- chloramphenicol- streptomycin- erythromycin- trimethoprim- nalidixin acid. Due to indiscriminate use of antibiotics has led to an increase in antibiotic resistance in bacteria.

Keywords: Antibiotics; Disc diffusion method; E. coli; hospital waste water; MDR.

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Planktonic Biodiversity and its importance role in fish production on Majalgaon Dam reservoir, Maharashtra State. India.

Sitaram B. Ingole, Prashant Sanghai*

Department of Zoology, Shri Siddheshwar College , Majalgaon, Dist. Beed. M. S. India Shivaji College Kannad Dist. Aurangabad.M.S. India*

ABSTRACT

Majalgaon Dam was constructed on the River Sindphana which is tributary of River Godavari, in Beed District (Maharashtra, India) in 1987. Which falls 16° 16 N latitude and longitude 73° 26 E. The River Sindphana has been under constant threat of pollution by sewage and industrial wastes, disposal of dead bodies, deforestation, excessive use of fertilizers and pesticides, bathing and water development programs. The dam has a catchment area is 3840 sq. km. It is of great Importance for the region because its water is used for human and cattle consumption, It is multipurpose type like irrigation and power production (Hydro Electric Project). As a representative of these ‘Majalgaon Dam’ was selected for the limnology studies. As a representative of these ‘Majalgaon Dam’ was selected for the limnology studies. The present study is aimed to investigate some of the important physical and chemical parameters along with the flora and fauna of the reservoir. A total of 33 species of phytoplanktons, 29 species of zooplanktons and . The reservoir is very productive. There are several types of fresh water fishes present in the dam. Labeo rohita, Cirrhina mrigal, Catla catla, Cyprinus carpio, Silver carp, Wallago attu, Mystancenbelus armatus, Notopterus chital, Barbus ticto, Channa staitus, Mystus seenghala, Mystus cavassius, Eutroplus suratensis, Belon concila, Chela, Tilapia mosambica, Rohtee alfrediana, Gobius giuris etc.17 species of fishes were identified during june2008-may2010.Hence the present work is an attempt to accumulate information pertaining to various aspect of hydrobiology of standing water bodies from this part of peninsular India.

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Department of Botany, Deogiri College, Aurangabad. Page 85

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Efficacy of lawsone on survival, growth and metamorphosis in fourth instars nymphs of Boisea coimbatorensis

Sujata Magdum and Rajashri Shinde Department of Zoology KRT Arts BH Commerce and A M Science College ( K T H M College), Nashik

ABSTRACT Understanding the insect responses to plant allelochemicals and pesticides in their local ecological context represents a key challenge in developing durable pest control strategies. Here an attempt is made to understand the effects (if any) after changing the natural food of Boisea coimbatorensis. To have the insight on this different dose of lawsone was administered to freshly moulted 24 to 36 hours old nymphs. These nyphms were topically treated on ventrolateral side of their abdomen with the help of Hamilton microliter syringe.

LC50 value was found to be 1.3μg/ml. Sublethal doses of lawsone (0.2 to 1.2 μg/ml) were used during experimentation and a control group was maintained for comparison. The results show that the sublethal concentration of lawsone significantly reduced metamorphosis of Boisea coimbatorensis. A low-lethal concentration of lawsone significantly increased nymphal development time, but significantly decreased emergence rate and longevity in metamorphosed 5th instar nymphs. Keywords: Lawsone; allelochemicals; Boisea coimbatorensis; Pulse dose exposure; Sublethal

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Preliminary studies on composition and diversity of beetles (Insecta : Coleoptera) in Nashik district, Maharashtra, India. Gurule Sachin 1, Supriya Vivek2* and Pawar Kajal 3 1, 2, 3 MVP’s K.R.T. Arts, B.H. Commerce and A.M. Science (KTHM) College, Nashik 422 002.

ABSTRACT Diversity of coleopteran beetles is assessed from Nashik district during August 2015 to February 2018 using random sampling method. Preliminary inventory of beetles has been prepared during study and total 32 genera recorded pertaining to 10 families of order Coleoptera viz. Scarabaeidae, Coccinellidae, Tenebrionidae, Chrysomelidae, Meloidae, Buprestidae, Carabidae, Cerambycidae, Elateridae and Dytiscidae. Of the total families recorded in the present study Scarabaeidae was found to be dominating with 11 genera and 12 species; while three genera each recorded from families Coccinellidae, Tenebrionidae and Chrysomelidae. Of the total 32 coleopteran insects recorded 17 insects were identified at species level while 13 insects were identified up to genus and given morphospecies label.

Keywords: Coleoptera, Beetles, Nashik, Diversity, Checklist

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TEMPERATURE INFLUENCE ON THE RATE OF DEVELOPMENT OF LIFE STAGES OF GRAM POD BORER, HELICOVERPA ARMIGERA (HÜBNER) (LEPIDOPTERA: NOCTUIDAE)

J.S. DAHEGAONKAR AND A.S. MOHITE

P.G. Department of Zoology and Research Academy, Sevadal College for Women, Nagpur-440009, India

ABSTRACT

Life stages of Helicoverpa armigera (Hübner) were held in constant temperatures ranging from 15oC to 30oC to determine the effect of temperature on development, oviposition and fecundity. Over the temperature range tested, developmental rate of the life history stages and oviposition period decreased as temperature increased from 15oC to 30oC.

By means of linear regression, a lower developmental threshold temperature of 11.5oC,

12.2oC, 13.4oC and 6.5oC was estimated for the incubation of eggs, development of larval stage, development of pupal stage and oviposition period, respectively. A mean thermal constant of 45.53 degree-days, 195.98 degree-days, 142.62 degree-days and 81.53 degree- days above estimated lower threshold temperature was required for the incubation of eggs, larval development, pupal development and oviposition period, respectively. Fecundity was positively correlated with temperature. Fecundity increases as temperature increases. Maximum oviposition occurred between 25oC and 27oC, however, temperatures of 30oC or higher were detrimental.

Keywords:Helicoverpa armigera, development, threshold temperature, degree-days

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BIOCHEMICAL VARIATIONIN FRESH WATER BIVALVE LAMELLIDENS MARGINALIS

S. G. Kamble, R. D. Sonwane and G. D. Suryawanshi

Department of Zoology, Yogeshwari Mahavidyalaya, Ambajogai, Dist. Beed-431517

ABSTRACT

The seasonal patterns of biochemical composition in bivalve, Lamellidens marginalis from different habitats from the Marathwada region were observed in different seasons. In Jayakwadi and Dhanegaon dam the protein level was found maximum during winter and low amount in Majalgaon dam mg/100gm of dry tissue weight. The glycogen level was maximum during winter in Dhanegaon followed by Jayakwadi and Majalgaon The lipid was more in Jayakwadi followed by Majalgaon, Nagapur and Dhanegaon dam in winter. The ascorbic acid was more in Jayakwadi followed by Majalgaon and Dhanegaon in winter. The bivalve mollusc shows maximum variation of biochemical composition as it undergoes different stages like development, maturation and spawning during different seasons and can be affected by environmental and internal factors, such as metabolic and physiological activities. It might be the spawning cycle and food supply are the main factors responsible for this variation

Keywords: Freshwater bivalves, L. marginalis, Protein, Glycogen, Lipid,

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Diversity of Some Lepidopterian Fauna of Chikhli Region Dist. Buldana Maharashtra State.

M.T. Nikam

Department of Zoology, Shri Shivaji Science and Arts College Chikhli, Dist. Buldana

ABSTRACT

Chikhli is located at 20.03°N 75.78°E. The climate is Cool and not so hot as compared to vidarbha region. The annual rainfall averages 800 mm.The Biodiversity of Butterflies was carried out in Chikhli region from September -2018 – December- 2018 using transect lining method. A total of 23 species of Butterflies belonging to 3 families were recorded during the present work. During the course of present studies it was observed that the family Nymphilidae represents by 9 species was the most dominant followed by Pieridae 7 species and Papilionidae 3 species. Peak activities of the butterflies were observed during the month of September. The present study help creating awareness about conservation of habitats of butterflies among local people.

Keywords: - Chikhli, Biodiversity, Lepidopterian, Butterflies

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ROLE OF CEREBRAL GANGLIA IN REGULATION OF OXYGEN CONSUMPTION OF FRESHWATER BIVALVE MOLLUSC, LAMELLIDENS MARGINALIS FROM GODAVARI RIVER AT KAIGAON DURING MONSOON SEASON

Deepak M Gaikwad

Rajarshi Shahu College Pathri Tq Phulambri Dist Aurangabad.

ABSTRACT

Considering the importance of neuro endocrine regulation on the metabolic processes in Lamellibranch molluscs, from freshwater environments, role of cerebral ganglia in respiratory metabolism of freshwater bivalve molluscs, Lamellidens marginalis from Godavari River at Kaigaon near Aurangabad. During monsoon season, 2006-2007 over a period of one year the adult bivalve molluscs, Lamellidens marginalis of 80-90 mm shell length were subjected to (a) control (normal) (b) ablation of cerebral ganglia (c) injection of distilled water and (d) injection of cerebral ganglionic extract for 10 days. The rate of oxygen consumption in bivalves from all four groups (including control) was measured on 2nd, 5th and 10th day. The study revealed that, the rate of oxygen consumption was significantly increased in injection of cerebral ganglionic extract, on 2nd,and 5th day compared to control. The rate also showed significant increase in ablation of cerebral ganglia on 2nd, 5th and 10th day.

Keywords: - Oxygen consumption, Cerebral ganglia, Lamellidens marginalis.

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In Vitro Effect of household pesticides and heavy metals on the growth of Azotobacter chroococcum and other microbes isolated from sewage irrigated fields.

Dr.Mrs. Jadhav P.N.

Department of Microbiology,Deogiri college,Aurangabad (M.S.), India 431001

______

ABSTRACT

In each ancient civilization there are signs of attempts for utilization of sewage for irrigation of agricultural crops. This practice has centuries long tradition in many countries in the world. In India sewage farming is practiced in many cities and towns and investigations on utilization of sewage for crop production have been done in India and abroad. In the present study effect of sewage components such as household pesticides and heavy metals was studied on the growth of Azotobacter chroococcum isolated from sewge irrigated fields. It was observed that phenyl highly reduces the number of Azotobacter chroococcum.Moreover heavy metals such as Mgso4, Feso4,Znso4,Hgcl2 appeared to be highly toxic to A.chroococcum.Agno3 was highly inhibitory when compared with other metals.Keywords—Sewage irrigation, household pesticides ,Azotobacter,heavy metals.

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“Effects of Estradiol hormone on the heart beat rate and oxygen consumption in freshwater Bivalve Lamellidens marginalis (Lamark)” Kayande H.G and Chavan R.J*

Deogiri College, Aurangabad *Dept. of Zoology, Dr. Babasaheb Ambedkar Marathwada University

ABSTRACT

In the present investigation effect of estradiol hormones on the heart beat rate and oxygen consumption in fresh water bivalve Lamellidens marginalis was studied. The fresh water bivalve mollusc, as a whole seem possesses considerably greater physiological susceptibility than that of marine mollusc. It is important to know that respiratory function of these animals is essential for understanding. In present study on the freshwater bivalve mollusc, showed that the heart rate (contraction of auricle) accelerates in due to injected of cardio excitatory drug As exposure period increase the rate of oxygen consumption decrease in both (i.e. control as well as experimental). Hence in the present study the heart beat rate and oxygen consumption was responded according estradiol injected.

Key Word: Estradiol hormone, Lamellidens marginalis,oxygen consumption, heart beat

Department of Botany, Deogiri College, Aurangabad. Page 93

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On A New Species of Genus Tylocephalum (Linton, 1890)(Cestoda: Lecanicephalidea) From Dasyatis Walga from Dist. Raigad (M.S) India

Vasant Dongare

S.M. Art's Commerce & Science College, Poladpur Ta. Poladpur Dist. Raigad (M.S) India

ABSTRACT

In the present study a new species is describe of Genus Tylocephalum (Linton, 1890) from Dasyatis walga at Borli, Dist Raigad (M.S) India, in the period of March 2017 to May 2018. The present worm scolex is oval, bearing four suckers, each sucker having oval shape; mature proglottids are three to four time longer than broad, testes are 60-70 in number, oval shaped, posterior testes are slightly larger in size than anterior testes, ovary is ‘V’ shaped, the lobes of ovary are elongated, broad anteriorly and narrow posteriorly The uterus is narrow anteriorly and broad posteriorly, starts from ootype straight reaches near to the anterior region of the segment.

Keywords:Tylocephalum, Dasyatis Walga, Raigad

Department of Botany, Deogiri College, Aurangabad. Page 94

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E-60

AVIAN POLYSPECIFIC CLUTCH SIZE AS BIOINDICATORS OF ECOLOGICAL CONDITION OF THE AREA

Chandrakant V. SIRSAT Department of Zoology, Vinayakrao Patil Mahavidyalaya, Vaijapur – 423 701,Dist. Aurangabad (MS) India

ABSTRACT

The present study deals with general trends in clutch-size of local birds and examined some of the hypotheses regarding the adaptive significance of the timing of breeding and variations in the clutch size, that have been put forth as possible explanations with in seasonal variation of average clutch-size of the birds. In the present work, recorded clutch size of 15 bird species belong to 10 families of 5 different orders most of them were passerines. Studies on clutch size helped to study life history traits of various birds, particularly Brahminy Myna Sturnus pagodarum and Indian Shikra Accipiter badius. Whereas clutch sizes of other birds provided an approximate measure about birth rate of that species. Clutch of each bird was observed approximately constant for that species. Further, this available information might be beneficial in future for detail study of that particular bird species from the local area. This study was carried out from July 2016 to June 2018 and simultaneously confirmed thereafter till June 2019.

Keywords : Clutch size, coefficient of variation, hatchling, nidicolous, atricial, precocious.

Department of Botany, Deogiri College, Aurangabad. Page 95

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E-61

Study of Antibacterial and Antioxidant activity of Arisaema tortuosum Schott

R.P.Patil, L.H.Kamble* and V.C.Patil**

Dept of Botany, Deogiri College, Aurangabad. *School of Life Sciences, SRTMU, Nanded. ** Dept of Botany, Dayanand College, Latur.

ABSTRACT

In this study, tuber extracts of Arisaema tortuosum were investigated for in-vitro antibacterial and antioxidant activity. The antibacterial properties were tested against five human pathogens such as Klebsiella pneumonae, Staphylococcus aureus, Escherichia coli, Proteus vulgaris, Pseudomonas aeriginosa using two solvents viz methanol and acetone by well diffusion method. The maximum antibacterial activity was observed in methanolic extract against all tested pathogenic strains. The antioxidant potential of Arisaema tortuosum was determined by using three methods viz DPPH, HRSA and FRAP assay. The results obtained from these three methods revealed % free radical scavenging activity in methanolic extract. In FRAP, methanolic extract shown higher antioxidant power than that of acetone extract. The standard antioxidant BHA at the same concentration exhibited a good deal of free radical scavenging percentage.

Department of Botany, Deogiri College, Aurangabad. Page 96

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E-62

Nutrient Dependent Microfaunal Diversity At Masoli Reservoir, Parbhani (M.S.), India

S. M. Yeole M. S. P. Mandal’s, Shri Shivaji College, Parbhani 431401

ABSTRACT

Water is the elixir of life. Most of the biochemical reactions that occur in the metabolism and growth of the living cells involve water, which has often been referred to as the universal solvent. Water is not only a major component but also the best solvent and a medium on which all organisms depend for their existence. Indeed the economics of all life processes and living communities are hinged on this most vital element. Zooplankton are microscopic free swimming animalcule components of an aquatic habitat which are primary consumers of phytoplankton. These provide the main food item of fish and can be used as indicators of the trophic phase of water body. In the present investigation, microfaunal study of Masoli reservoir has been estimated to form a link between nutrient levels and diversity of zooplankton. Study has been carried out for the period of one year i.e., from February 2018 to January 2019 on monthly basis. The findings are discussed in full length paper. Keywords:- Zooplankton diversity, Nutrient level, Masoli reservoir.

Department of Botany, Deogiri College, Aurangabad. Page 97

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E-63

Survey of helminth parasites in freshwater fishes from Marathwada region, MS, India

Ravi Solunke Department of Zoology, Dayanand Science College, Latur

ABSTRACT The present study deals with the survey of helminth parasites from Marathwada region(M. S.) India, during July 2018 to June 2019 this report summarizes the data of incidence,intensity and density of infection of helminth parasites in freshwater fishes in relation toenvironmental factors. Fish samples were collected from four main localities i.e. Osmanabad,Aurangabad, Latur and Nanded in Marathwada region examined for helminth parasites includedthree classes i.e. Cestode, Trematode and Nematode. During the present study 879 fishes wereexamined, in which 487 fishes were infected with seven genera of helminth parasites amongthese four were cestodes, two were trematodes and one was nematode. The present studies arehelpful for the status of diversity of helminth parasites from Marathwada region.

Keyword: Survey, Helminth parasites, Freshwater fishes, Environmental factor, Marathwada.

Department of Botany, Deogiri College, Aurangabad. Page 98

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Study of alimentary tract of Channa punctatus from Kaigaon Toka, Aurangabad.

Ajit K. Gedam

Jijamata college, Bhenda factory, Ahmadnagar

ABSTRACT

The alimentary tract of teleosts has attracted considerable interest because of its diversity of form, related to diet. Therefore, the gross morphology and histology of the tract has long been a topic of investigation and the present study reveals information on alimentary tract of Channa punctatus. While observing the morphological characters of alimentary canal of Channa punctatus it is found that the alimentary canal is demarked into oesophagus, stomach, pyloric caecae, intestine and rectum. The mean alimentary canal length (ACL) observed was 17.4 ± 1.57cm. (Table 3.1).

Keywords: Channa punctatus, oesophagus, stomach.

Department of Botany, Deogiri College, Aurangabad. Page 99

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“Study on zooplankton diversity from Rui Project,Osmanabad(MS) India”

Afreen Rehman Deepak Gaikwad and Sunil Sherkhane Dept. of Zoology, Kohinoor College, Khultabad, Dist. Aurangabad (M.S) India. Dept. of Zoology, RajashriShahu Art’s, Commerce and Science College Pathri, Tq. Fulmbri, Dist. Aurangabad, Dept. of Biology, Godavari College, Aurangabad (M.S) India

ABSTRACT

The present communication deal with the study of Zooplankton diversity in Rui project, Osmanabad. All these species of Zooplanktons belongs to class Rotifera and Crustacea. The class Rotifera was represented by seven species viz. Bronchionuscaudatus, Euchlanisdilatata, Keratellatropica, Lecaneluna, Monostylla bulla, Ploesomadenticulare, and Cephalodellaauriculata,The class Crustaceawas represented by fourteen species viz. Daphnia carinata, Crinodaphniareticulata, Moinadubia, Alonaaffinis, Graptolebristestudinaria, Cyprissubglobosa, Cyprettaintonsa, Metacyprismaracasensis, Diaptomusgracilis, Heliadiaptomusviridisa, Macrocyclopsdistinctus, Mesocyclopsleucarati, Nanopuspalustris and Mesochra rapines.The present study aims at providing a preliminary knowledge on the productivity and diversity of zooplanktons which can be utilized during the formulation of management measures to improve the productivity of the reservoir.

Keywords: Rui Project, Zooplankton, Diversity.

Department of Botany, Deogiri College, Aurangabad. Page 100

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E-66 Redescription of the plant nematode Helicotylenchus vulgaris Yuen, 1964,(Nematoda: Hoplolaimidae)

Deepak Gaikwad

Dept. of Zoology, RajashriShahu Art’s, Commerce and Science College Pathri, Tq. Fulmbri, Dist. Aurangabad, India

ABSTRACT

In the present investigation a taxonomy study is done in plant parasitic nematode of genus Helicotylenchus. Thesoil and root samples were collected from rhizosphere of different plants and different localities of Fulambri Dist. Aurangabad, (M.S) India. Female body shape spiral, annules distinct excretory pore located just anterior to level of oesophageal intestinal junction, distinct, widening somewhat immediately below cuticle. Vulva with epiptygmas folded in, as is typical for the genus; sides somewhat raised, resembling vulval flaps. A known species of Helicotylenchus vulgaris (Yuen, 1964) were identified from the collected material.

Keyword:Helicotylenchus, Phulambri, Taxonomy

Department of Botany, Deogiri College, Aurangabad. Page 101

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APPLICATION OF CORRELATION TECHNIQUES OF IMAGE PROCESSING DATABASE FOR SNAKES IDENTIFICATION

1Yogesh Reddy, 2Anilkumar Pardeshi, 3Nilesh Pakhare, 4Utkarsh Jadhav, 5Shubham Malekar PG. Dept. of Zoology, Deogiri College, Aurangabad, 431005 (MS)

ABSTRACT Snakes are very useful animal in ecology, farming and also in medicine, but now a days this animal comes under Wild Life Protection Act 1972 because of their decreasing population. The present study is an attempt to identifiy the Snakes through database by using Correlation Techniques of image processing. Object detection and recognition based on image processing is vastly concentrating field in research. The motivation for this study is to build data based system for detecting and recognizing snakes through scales for the animal biodiversity researchers. The animal detection and recognition is an important area which has not been discussed. Technology used in this research can be modified further to use in applications such as security and monitoring purposes etc.

Keywords:Snake, MAT LAB, Identification.

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Determination of the prevalence of Babesia DNA in the blood samples of Goat from Aurangabad by PCR.

V.S. INGLE, V.R. MORE. V.S. INGLE: Research scholar, Zoology Dept, Govt. college of Arts & Science, Aurangabad. V.R. MORE: Associate prof. Zoology Dept., Govt. college of Arts & Science, Aurangabad.

ABSTRACT Babesiasis is a zoonotic disease caused by Babesia species. Babesia species are protozoan parasites that parasitize the red blood cells of domestic animals and humans causing anemia in the host. The purpose of current study was to determine the presence of Babesia DNA in the blood samples obtained from goats from Aurangabad, Maharashtra, India. Blood samples were collected from 100 Goats from randomly selected herds located in the main livestock production regions of Aurangabad. PCR was used to detect babesia DNA in the blood samples whereby an amplified band size of 428 bp was considered positive for Babesia. The results indicate that 2% of the blood samples were positive for Babesia species.

Keywords: Babesia spp, Goat, PCR, Zoonotic.

Department of Botany, Deogiri College, Aurangabad. Page 103

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E-69

Labelling of Spider with DNA barcode”

Nagawanshi M N* Khedkar G D**

P.G Dept. of Zoology, Deogiri College Aurangabad (MS) India

** PHCDBBDS, Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad

ABSTRACT

COI gene of mitochondria used to identify unique sequence for DNA barcode. Spiders are found to habitat specific. Morphological characters are misguiding due to moulting stages of juveniles. Molecular signatures contribute assigning spiders as true species and provide guidelines for researchers. We have identified spider species belonging to different families.

Department of Botany, Deogiri College, Aurangabad. Page 104

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E-70

Nutrient Dependent Microfaunal Diversity At Masoli Reservoir, Parbhani (M.S.), India

S. M. Yeole M. S. P. Mandal’s, Shri Shivaji College, Parbhani 431401

ABSTRACT

Water is the elixir of life. Most of the biochemical reactions that occur in the metabolism and growth of the living cells involve water, which has often been referred to as the universal solvent. Water is not only a major component but also the best solvent and a medium on which all organisms depend for their existence. Indeed the economics of all life processes and living communities are hinged on this most vital element. Zooplankton are microscopic free swimming animalcule components of an aquatic habitat which are primary consumers of phytoplankton. These provide the main food item of fish and can be used as indicators of the trophic phase of water body. In the present investigation, microfaunal study of Masoli reservoir has been estimated to form a link between nutrient levels and diversity of zooplankton. Study has been carried out for the period of one year i.e., from February 2018 to January 2019 on monthly basis. The findings are discussed in full length paper.

Keywords:- Zooplankton diversity, Nutrient level, Masoli reservoir.

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“HEMIPTERA: DISTRIBUTION, OCCURRENCE, TYPE OF DAMAGE AND SYMPTOMS ON MULBERRY (MORUS ALBA L.)” Avhad Sunil. B. &Hiware Chandrashekhar. J Assistant Professor, Department of Zoology, Annasaheb Vartak college of Arts, Kedarnath Malhotra College of Commerce, E.S. Andrades College of Science, road, Palghar (M.S), India. & Professor, Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad (M.S), India

ABSTRACT Mulberry (Morus spp.) leaf is the only natural food for the silkworm, Bombyx mori L. It is a perennial, evergreen, luxuriant crop cultivated in all types of soils, both under rainfed and irrigated conditions. So far, over 300 insect and non-insect species of pests are known to infest mulberry in varying intensities during different stages of the crop and seasons. The present investigation shows taxonomical study on 9 species Hemipteran insect pests namely Aonidiella qurantii, Dysdercus singulatus, Empoasca flavescens,Maconellicoccus hirsutus, Nezara viridula L.,Oxyrachis tarandus and Tessaratoma javanica,Erthesina acuminata Dallas, 1851 and Hotea curculionoides of mulberry garden from various sites in Aurangabad district, Maharashtra, India. In present work during study period some important Hemipteran pests of mulberry are described with respect to distribution, occurrence and type of damage and symptoms.

KEYWORD: Taxonomy, Distribution, Damage and symptoms.

Department of Botany, Deogiri College, Aurangabad. Page 106

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In Vitro Effect of household pesticides and heavy metals on the growth of Azotobacter chroococcum and other microbes isolated from sewage irrigated fields.

Dr.Mrs. Jadhav P.N.

Head, Department of Microbiology, Deogiri college, Aurangabad (M.S.), India 431001

______

ABSTRACT

In each ancient civilization there are signs of attempts for utilization of sewage for irrigation of agricultural crops. This practice has centuries long tradition in many countries in the world. In India sewage farming is practiced in many cities and towns and investigations on utilization of sewage for crop production have been done in India and abroad. In the present study effect of sewage components such as household pesticides and heavy metals was studied on the growth of Azotobacter chroococcum isolated from sewge irrigated fields. It was observed that phenyl highly reduces the number of Azotobacter chroococcum. Moreover heavy metals such as Mgso4, Feso4,Znso4,Hgcl2 appeared to be highly toxic to A.chroococcum.Agno3 was highly inhibitory when compared with other metals.

Keywords: Sewage irrigation, household pesticides , Azotobacter, heavy metals.

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“POPULATION FLUCTUATION OF HELICOTYLENCHUS STEINER, 1945 IN RELATION TO SOIL ABIOTIC FACTOR IN MULBERRY FIELD”

Avhad Sunil. B&Hiware Chandrashekhar. J Assistant Professor, Department of Zoology, Annasaheb Vartak college of Arts, Kedarnath Malhotra College of Commerce, E.S. Andrades College of Science, Vasai road, Palghar (M.S), India. & Professor, Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad (M.S), India

ABSTRACT In the present study, the monthly population fluctuation of Helicotylenchus Steiner, 1945 was observed in relation to soil temperature, moisture and pH in a mulberry (Morus alba L.) field with immense economic importance in the sericulture. The objective of study is to understand the influence and effect of these soil abiotic factors on the population of these plant parasitic nematodes and Correlation coefficients (r) between mean population Helicotylenchus spp. and different soil abiotic factors in Aurangabad Mulberry garden.

KEY-WORDS:Helicotylenchus Steiner 1945,Population fluctuation, soil abiotic factors.

Department of Botany, Deogiri College, Aurangabad. Page 108

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E-74

Leidynema appendiculata (Leidy,1850) Chitwood,1932. (Oxyuroidea: Thelastomatidae) found infecting Cockroaches Periplaneta americana (Dictyoptera:Blattidae ) from Aurangabad city,(MS), India.

Gavali S.V. Dr. Ashok Mote*,Dr. S .N. Borde**

*Sant Ramdas college Ghansavangi,Ta.Ambad,Dist.Jalna **Dept. of Zoology, Dr .Babasaheb Ambedkar Marathwada University Aurangabad.

ABSTRACT

The present study is carried out to detect and isolate the intestinal entomoparasitic nematodes from Periplaneta americana. Cockroaches were collected from different residential areas of Aurangabad, identified and dissected. Nematode found infecting hind gut of cockroaches Periplaneta americana resembles to those of Leidynema appendiculata in all their morphological features. Females were found 0nly . Measurements and camera Lucida drawings are also given.

Department of Botany, Deogiri College, Aurangabad. Page 109

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E-75

Morphological Studies of Chilodonella Cyprini(Moroff 1902) from freshwater fishes

T.T. Shaikh

ABSTRACT

In the present study morphology of Chilodonella cyprinid from fresh water fishes have been described, occurring in particularly on the gills and in a later stage on its skin. The relative prevalence of this protozoan parasite among different fresh water fishes from Aurangabad were analysed. Overall percentage of prevalence for the whole year was 41.55 %. This parasite showed highest prevalence in the months of November to February, moderate in July to October whereas minimum prevalence was recorded in the March to June.

Keywords: Chilodonella cyprini , gills, fishes, prevalence

Department of Botany, Deogiri College, Aurangabad. Page 110

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ON A NEW SPECIES OF GENUS DAVAINEA (BLANCHARD, 1891) FROM GALLUS DOMESTICUS AT GANGAPUR, AURANGABAD, M.S., INDIA.

Dr. Ishrat Vasi Shaikh* Department of Zoology, Abeda Inamdar Senior College, Azam campus, Camp, Pune - 411001 (India).

ABSTRACT The present paper contains the description ofD.govindi, a new species of genus Davainea(Blanchard, 1891). Seven specimen of cestode parasite collected were flattened, preserved in 4% formalin, stained with Harris Haematoxylin, dehydrated through various alcohol grades, mounted in DPX and whole mount slides were prepared for detailed anatomical studies. The drawings were made using camera lucida. All measurements are in millimeters, unless otherwise indicated. The present species differs from earlier reported species in having large, oval scolex with foursuckers; numerous rostellar hooks in two circles; testes 40 –50 (45) arranged in single field; cirrus pouch medium, elongated, obliquely placed, ovary large, central, distinctly bilobed, each lobe with 5-7 blunt acini; ootype, large, oval, compact, post-ovarian; vitelline glandtriangular, compact, post-ovarian.

Key Words: Cestoda, D. govindi n. sp., Gallus domesticus, Gangapur, Aurangabad.

Department of Botany, Deogiri College, Aurangabad. Page 111

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E-77

On A New Species of Genus Tetragonocephalum (Shipley & Hornell, 1905)(Cestoda: Lecanicephalidea) From Dasyatis Walga from Dist. Raigad (M.S) India

Vasant Dongare and Yogesh Reddy

S.M. Art's Commerce & Science College, Poladpur Ta. Poladpur Dist. Raigad (M.S) India

Dept of Zoology, Deogiri College, Auranagabad (M.S) India

ABSTRACT

In the present study a new species of cestode parasite Tetragonocephalum shriwardhanensis n. sp. from Dasyatis bleekeri was described at Shriwardhan, Dist. Raigad

West coast of (M.S) India, in the period of March 2017 to May 2018. The scolex consisting of anterior rounded knob and a posterior collar like cushion, its quadrangular in shape,mature segments are almost squarish in shape broader than long, slightly broader, with convex lateral margins, testes are preovarian and upper side of the proglottids. The cirrus pouch is large in size oval in shape, cirrus is coiled like long structure broad at the anterior end, narrow at the posterior end, ovary is compact, dumb-bell shaped; each lobe is large placed at posterior end of the segments,uterus is zigzag tube like structure spread to the posterior end of the proglottid,

Key Words:Tylocephalum, Dasyatis Walga, Raigad

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Department of Botany, Deogiri College, Aurangabad. Page 113

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ANALYSIS OF HAIR SAMPLE OF SOME WILD ANIMALS IN RELATION WITH DIAMETER AND MEDULLARY PATTERNS USING MICROSCOPIC TECHNIQUE

Yogesh Reddy and A.B. Pardeshi

PG Dept of Zoology, Deogiri College, Aurangabad (M.S) India Email: [email protected]

ABSTRACT

As the wild animals poaching are increasing, it becomes task of forensic experts to ascertain their individuality. The microscopic investigation of animal hair samples may provide valuable information helpful for both biology and forensic laboratory science. In the present investigation a microscopic studies of hair samples of some wild animal were evaluated. Hair samples collected were investigated for cuticle, cortex and medulla. The obtained result of medullary index can be use as valuable tool for species identification of wild animals, victim in poaching and wild life crimes. The technique used in this study can be easily, quickly and economically applied in routine investigation, keeping the time required identifying a sample at the minimum, this are essential prior to DNA analysis.

Key Words: Wild animal hairs, Microscopy, Medullary index, Diameter

Department of Botany, Deogiri College, Aurangabad. Page 114

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E-80

ISOLATION, CHARACTERIZATION AND INSECTICIDAL POTENTIAL OF BACILLUS SUBTILIS AND ALLOIOCOCCUS OTITIS AGAINST HELICOVERPA ARMIGERA HUBNER.

Kalane V. G1., Jadhav S. M.2, Jadhav P. N.3 and Pardeshi A. B.4 1,3,4P. G. Department of Zoology,Deogiri collage Aurangabad 2 Dept. of Zoology, S. P. College, Pune

ABSTRACT

Helicoverpa armigera Humbner is one of the most destructive pest of cotton. Damage caused in spite of various control methods, the discovery of new entomopathogenic bacterial species and isolates bearing insecticidal traits against novel targets is needed in the near future. In this study, isolation, characterization ofAlloiococcus otitisand Bacillus subtiliswas assessed for insecticidal activity against Helicoverpa armigera. Bacterial isolation were performed from dead and alive larvae of Helicoverpa armigera, bacterial isolates were characterized based on their morphological and biochemical characteristics. The application of concentration were conducted with five different bacterial concentrations (8.5 x 107 cfu/ml, 12.2 x 107 cfu/ml, 16 x 107 cfu/ml, 20.2 x 107 cfu/ml and 24.4 x 107 cfu/ml) of the active cells and for insecticidal activity 1 ml of bacterial suspension were performed on the third instar larvae of Helicoverpa armigera with the leaves of cotton plant.

The sub lethal and median lethal dose of Bacillus subtilis was LD10 = 31.61 cfu/ml and

LD50 = 113.9 cfu/ml at 96 hrs and for Alloiococcus otitis was LD10 = 89.73 cfu/ml and LD50 = 209.6 cfu/ml at 96 hrsagainst third instar larvae. Result revealed that, the mortality increase with increase in concentration of bacterial populations. The highest mortality of Helicoverpa armigera is found by bacterial strains,Bacillus subtilisat 20.2 x 107 cfu/ml and for Alloiococcus otitis at 24.4 x 107 cfu/ml. Stastical variance, 95% confidence limits and regression equations are presented. Key-words:Alloiococcus otitis, Bacillus subtilisand Helicoverpa armigera.

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INFLUENCE OF PHYTOECDYSTEROID ISOLATED FROM DALBERGIA SISSOO ON ECONOMIC PARAMETERS OF SILKWORM, BOMBYX MORI L.

Barge S. B., Vetal D. S., Nagare G. J., and Pardeshi A. B.

P. G. Dept. of Zoology, Deogiri College, Aurangabad, Maharashtra

ABSTRACT The phytoecdysteroid isolated from the plant, Dalbergia sissoo Roxb. and were tested against 4th and 5th instar larvae of silkworm for improving the performance of growth and cocoon characteristics of silkworm, Bombyx mori L. The various concentrations of Dalbergia sissoo plant extract (5, 10, 15, 20, 25 mg/ml.) were administered to 4th and 5th instar silkworm with mulberry. The larval weight, cocoon characteristics were influenced by various concentration of plant extract. The intensity of influence was depends on the time and dose exposure. The plant extract at 25mg/ml. concentration resulted higher larval growth and increased cocoon weight. The mean larval weights, relative growth rate of silkworm, Bombyx mori were increased. The average pupa weight, shell weight, shell ratio and silk filament length were also increased with this supplementation of plant extract over the control. In the present study the plant extract of Dalbergia sissoo have growth promoting effect in silkworm which helps to improve the performance of silk in Bombyx mori. Keywords: Plant extract, Bombyx mori, parameters.

Department of Botany, Deogiri College, Aurangabad. Page 116

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E-82

Histopathological Changes in Labeo rohita Exposed to Mercury with Banana Peel as Bioadsorbent

S. A Shaikh

Department of Zoology, Deogiri College, Aurangabad, 431005 (MS)

ABSTRACT

An increase in water pollution due to severalheavy metals released from industries and pesticide from the field is a threat to aquatic organisms. Among aquatic organisms fishes are the good source of protein and vitamins to human being, they are eaten by humans and this heavy metals through food chain, gets transferred to the humans.The present work deals with the study of natural products which are usually consider as waste such as banana peels, has been used as good adsorbent of heavy metals and pesticides. Banana peels contain nitrogen, Sulphur and carboxylic acid, the acid are responsible for the peels ability to blends with toxic elements andremove from the water.In the present experiment it was observed that the effect of heavy metals mercury in the tissue of fishLabeorohita getreduced when banana peels used as abioadsorbent.

Keyword:: Mercury, banana peel, bioadsorbent

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BIOPESTICIDAL ACTIVITYOF CASSIA TORA AGAINST RED COTTON BUG, DYSDERCUS CINGULATUS FAB.

Gadewad M. G.1, Jadhav S. M.2 and Pardeshi A. B.2, 1,3Dept of Zoology, Deogiri College, Aurangabad 2Dept. of Zoology, S. P. College, Pune

ABSTRACT

Insecticidal effect of methanol and ethyl acetate leaves extract of Cassia tora were studied against Dysdercuscingulatus. The plant leaves were dried, powdered and extracted in soxhlet apparatus in methanol and ethyl acetate solvent for 24 hrs. The adult red cotton bug, D. cingulatuswere exposed to various concentration and percent mortality were recorded after 96hrs

The insecticidal activity of leaves extract of Cassia tora were LD10= 4.871μg/gm.

LD50= 8.500μg/gm., LD90=13.09μg/gm., LD99= 13.71μg/gm. in methanol and LD10=

2.597μg/gm., LD50= 6.096μg/gm., LD90= 13.05μg/gm., LD99 = 13.98μg/mg. in ethyl acetate. Results revealed that the mortality increase with increase in concentration of the plant extract. The ethyl acetate solvent extract showed more insecticidal property againstDysdercuscingulatus. Stastical variance, 95% confidance limits and regression equations are presented.

Keywords:Dysdercuscingulatus,Cassia tora, mortality.

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Natural saponin in Sapindus emarginatus controls the protein loss in Phaseolus aconitifolius grains caused by Callosobruchus chinensis

Bajad P. N.1, Pardeshi A. B.2 and Pagore V. P.3

2Dept. of Zoology, Deogiri College, Aurangabad

1,3Shri. Muktanand College, Gangapur

ABSTRACT Saponin containing Pericarp of Sapindus emarginatus fruit were tested for their effect on Callosobruchus chinensis. It is most widespread and dreadful pest. The whole grain of Phaeseolus aconitifolius were found to contain 23.3 % protein, while the infected hollow grains had reduced protein contents. On exposure to ethanol, methanol and aqueous extracts of Sapindus emarginatus, the percent protein loss in total grains at the said concentrations was highly reduced indicating the saving of the proteins against the damage by Callosobruchus chinensis. Protein loss was also minimized in different concentrations of ethanol, methanol and aqueous extracts. keywords: saponin, Callosobruchus chinensis, Phaeseolus aconitifolius

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E-85

TO STUDY THE ENDOPARASITES IN FRESHWATER FISHESMASTACEMBELUS ARMATUS, CHANNA GACHUAIN MAHARASHTRA, INDIA.

Laxmi Indani and S. A. Shaikh

Dept. of Zoology, Deogiri College, Aurangabad, 431005 (MS)

[email protected]

ABSTRACT

Fishes are nutritional food for human being since human utilizes these fishes in their diet, it is important that fish should be healthy and free of infection. In the present work taxonomical study was done of some endoparasite from freshwater fish Mastacembelus armatus and Channa gachua collected from the local fish market of Aurangabad. It was observed the both freshwater fish species were infected with endoparasites which were further identified as cestode:genus Senga and trematode genus Azygia.

Keywords:Mastacembelus armatus, Channa gachua, Cestode, Trematode

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E-86

Revision of the Genus Xiphocephalus and Description of Xiphocephalus arnatakaensis (Apicomlexa: Eugregarinida : Stylocephalidae) From Tenebrio molitor (Coleoptera: Tenebrionidae), In Jalna district, Marathwada, M.S. India

Kanse V. S.*, Nagawanshi M.N **, Pardeshi A.B.*** and Chondekar R.P.**** *Asst. Prof. Dept. of Zoology, Deogiri College, Aurangabad , 431004 (M.S.) **Asso Prof. Dept. of Zoology, Deogiri College, Aurangabad , 431004 (M.S.) ***Prof. and Head .Dept. of Zoology, Deogiri College, Aurangabad , 431004 (M.S.) ****Asso. Prof. Dept. of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004 (M.S.)

ABSTRACT Xiphocephalus is revised, xiphocephalus karnatakaensis (Apicomplexa: Eugregarinida) is described from Tenebrio molitor(Coleoptera: Tenebrionidae) collected from the atDaithana, ta-Partur, District Jalna, Marathwada, Maharashtra State, India. Developing trophozoites solitary :Epimerite is long, xiphoid, anterior end is sharp and pointed. It measures about 16.8 to 21.2 um (18.2 um) in length and 4.6 to 9.2 um (7.5um) in width. Daimerite is long, tube like. Epimerite - diamerite septum is clearly marked. Protomerite is broad and spherical, cytoplasm is brown with large granules. Deutomerite is elongated and cylindrical. Anterior end is wide, posterior end is slightly tapering. Gamont of this species is very long, broad and cylindrical structure. It measures about 612.0 to 1368.0 um (908.16 um) in length. In mature gamont epimerite is absent. Protomerite is spherical to hemispherical. Deutomerite of this species is very long, broad and cylindrical structure. It is solitary with thick granular cytoplasm which is dark brown in colour. Protomerite - deutomerite septum is clearly marked. Shape of nucleus is ellipsoid. It is centrally to posteriorly placed. Gametocyst is large spherical to oval structure with large spherical to oval nucleus. It measures about 144.6 X 162.7 um. Oocyst of this species is stone or seed shaped. It measures about 9.44 X 12.0 um. Association or syzygy of this species is lateral. All measurements are in micron. Keywords:Apicomlexa,Gregarines, Xiphocephalus, karnatakaensis, Coleoptera: Tenebriodae Eugregarinida Gametocysts and Oocysts etc.

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E-87

Prevalence and seasonal rate of infestation of helminth parasite in fresh water fish in Aurangabad region (MS) India

Jyoti shirsat and V.R More

Department of Zoology, Government College of Arts and science Aurangabad (MS) India.

Email Id: [email protected]

ABSTRACT

A study was undertaken to investigate the prevalence, mean intensity, abundance and f seasonal rate of infestation of helminthes s parasites in fresh water fish Mastacembalus armatus .association with a nematode species . In all 151 specimens of Mastacembalus armatus were examined , out of which 89 were found infected by nematode species , The site occupied by the parasite is mid gut and hind gut region of intestine and liver but in heavily infected fish the parasite occur throught the length of gut . The study reveals that helminthes show maximum prevalence and seasonal rate of infestation in summer season(75%) followed by winter season (56.6%)and minimum in rainy season (40.47%).

Keywords : Helminthes , infestation, intensity ,prevalence, nematodes

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E-90

Preliminary studies on composition and diversity of beetles (Insecta :Coleoptera) in Nashik district, Maharashtra, India. GuruleSachin1, Supriya Vivek2* and PawarKajal3

1, 2, 3 MVP’s K.R.T. Arts, B.H. Commerce and A.M. Science (KTHM) College, Nashik 422 002. 1 [email protected] Mobile No.: 9764215025 2 * Corresponding author: [email protected] Mobile No.: 8698455146 3 [email protected] Mobile No.: 7507729164

Abstract Diversity of coleopteran beetles is assessed from Nashik district during August 2015 to February 2018 using random sampling method. Preliminary inventory of beetles has been prepared during study and total 32 genera recorded pertaining to 10 families of order Coleoptera viz. Scarabaeidae, Coccinellidae, Tenebrionidae, Chrysomelidae, Meloidae, Buprestidae, Carabidae, Cerambycidae, Elateridae and Dytiscidae. Of the total families recorded in the present study Scarabaeidae was found to be dominating with 11 genera and 12 species; while three genera each recorded from families Coccinellidae, Tenebrionidae and Chrysomelidae. Of the total 32 coleopteran insects recorded 17 insects were identified at species level while 13 insects were identified up to genus and given morphospecies label.

Keywords: Coleoptera, Beetles, Nashik, Diversity, Checklist

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Theme F Ethnobotany, Biopesticide and bioactive compounds

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Ethno veterinary medicine from Hingoli district, Maharashtra India

Chavan R.T.

Toshniwal Arts, commerce and science college, dist. Hingoli

ABSTRACT

The Hingoli division is dominated by several tribal groups. The ethnic communities like Andh, , Banjara and pangul inhabit in Hingoli regions. Hingoli region of Maharashtra rearing of cattle, Goats, Buffalo, sheep, fowls etc. are common practice among the tribal society. The animal disease is treated by the application of herbal drugs as the ethnic communities have their own system of herbal veterinary medicine practiced since time immemorial. The common cattle disease is anthrax, ectoparasites, helminthiasis, constipation, diarrhea, dysentery, mastitis, FMD etc. during this field study 20 ethno veterinary prescription have been recorded in which 20 plants species belonging to 10 families have been used. In the present paper scientific and vernacular name of the plants the made of preparation of medicine have been given. The use of plants reveals their interest in ethnomedicine and further research on these species could lead to the discovery of novel bioactive molecules for efficient management of diseases.

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F-1 a

Significance of indigenous medicinal plant and their utilisation

Prof. Bhagwan M. Waghmare

Botany Research Centre, Department of Botany, Maharashtra Mahavidyalaya, Nilanga. Dist. Latur.

*Department of Botany, Baliram Patil Mahavidyalaya, Kinwat Dist. Nanded*

ABSTRACT

The present investigation includes underground plant parts such as,tubers, roots, rhizome and corm of 10 indigenous ethno-medicinal plants were utilized for management of pathogenic diseases and the common ailments of peoples which caused by pathogenic microbes. Underground parts of the ethno-medicinal plants were collected from Kinwat forest which were belongs to Amorphophallus campanulatus, A. sylvaticus, Corollocarpus epigaeus, Habenaria grandifloriformis and Root of Asparagus racemose, Celosia aregentea, Cryptolepis buchnanii, leea macrophilla, Radermachera xylocarpa, Curcuma pseudomonatana and their significant ethno-medicinal knowledge was gathered during the field exploration trips with various tribal vaidus, Janta, healers and regular BAMS practitioners as well as elderly people in and around the Kinwat habitats and the results were recorded. The Kinwat region is very rich for ethano-medico-botanical point of view and it is essential to know about the important to the Identification,documentation,conservation, protection and scientific utilization of the medicinal plants for phyto-social importants. The medicinal plants belongs to different families and their parts, citation and socioimportance are remarkable according to the screened parts of tested plants. The parts of screen plants are highly beneficial for management of the common aliments of human being such as curmicide, apetiser, hipatospllenomegalis, aprodiac, against snak ebite ,rhemutism also dysentery.Whereas,root part of some of the plants are highly significant to cure Simillarly ,root of the Leea psychiatric and heamophilic disorder also aphrodisiac dermotherapeutic, and vermicide. macrophilla used as anti-cancer,anti-viral and other common dieases of the human being.

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F-2

Pharmacognostic studies of Sphagneticola trilobata[L.] Pruski. Asteraceae

Sangeeta S. Sutar

Department of Botany Sir Sayyed College, Aurangabad

ABSTRACT

Sphagneticola trilobata is a perennial herb, it belongs to the tribe. Heliantheae of family Asteraceae. S. trilobata has traditionally been used to treat infections, indigestion, hepatitis and piles, it shows antibacterial and antifungal activity. To standardize and differentiate the species anatomical features such as anatomy of nodes, petioles and leaves and dermatological parameters were screened. In addition to these parameters the species were screened phytochemically for major chemical groups such as volatile oil, starch ,proteins, tannin, saponins, fats, glucosides and alkaloids.

Keywords: Pharmacognosy, Sphagneticola

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F-3 Survey of Aquatic and Submerged Aquatic Medicinal plants from Rahati area of Parbhani. (MS)

Kondekar Chaitali and Dr. Syed Sabiha

Department of Botany, Shri. Shivaji College, Parbhani, (MS) India.

ABSTRACT

The aquatic plants are used as natural as well as traditional medicinal source from ancient time. Each ethnic community have their own and unique health care system refers as “Ethno-therapeutics”. In Rig-Veda it is mentioned that, the plant part like root, stem, leaves, fruit and rhizomes used in treatment for preventing diseases and used as an ointment (Shankar and Mishra 2012). The current paper deals with study of selected aquatic and submerged aquatic medicinal plants like Cyperous rotundus L., Commelina benghalensis Linn., Bacopa monnieri Linn., Typha angustifolia L., Eichhornia crassipes (Mart) Solms and Nymphea mexicana Zucc.,etc. used by local peoples of Rahati area to cure various ailments.

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F-4

Review: Ethanobotanical properties of Turmeric in Maharashtra

Sunita Bhosle, Smita Basole and Savita Sukte

Balbhim Arts, Science and Commerce College, Beed

ABSTRACT

Ethanobotany is a recent branch of Life science leading to various disciplines such as anthropology, archeology, botany, ecology, economics and medicine, religious, cultural and several other disciplines. Seeing the Indian history it is found that Ayurveda plays major role in our country, herbal drugs are found to used very popularly not only in India but worldwide it is accepted. Today Ayurveda and scientific investigations are going hand in hand and working for new achievements. Turmeric (Curcuma longa and Curcuma aromatica Salisb.) has been described in Ayurveda, as treatment inflammatory diseases and is referred by different names in different cultures, active principle called Curcumin or diferuloylmethane, a yellow pigment present in turmeric (curry powder) has been shown to exhibit numerous activities. Curcumin is the major component found in Turmeric and is interested area in between the researchers. The present study was aimed to review the ethanobotanical properties,phytochemical and pharmacological properties of turmeric plant. The most important part of turmeric is rhizome part widely used by different tribal communities. Turmeric exhibits biological actions, which include anti-inflammatory, anti-diabetic, analgesic, antibacterial, anti-fungal, anti-protozoal, anti-ulcer, hypocholesteremic activities also turmeric has shown anti- cancer effect induced mainly mediated through induction of apoptosis and many more medicinal values. Maharashtra state in India ranks sixth in area under turmeric cultivation. In Maharashtra Sangali, Satara, Hingoli, Nanded, Parbhani are the major turmeric growing districts. , a city of Maharashtra, is second only to Erode in size and importance as a production and trading site for turmeric.

Keywords: Ethanobotany, Ayurveda, Turmeric, Curcumin, Rhizome, Medicinal

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F-5

Efficient Synthesis of 2, 4, 5- Triarylimidazoles: A Bioactive Molecule

Arshia Parveen

Department of Chemistry, B.Raghunath ACS College, Parbhani-431401.

ABSTRACT

Ceric ammonium nitrate catalyzed efficient synthesis of bioactive 2,4,5- triarylimidazoles using benzil or benzoin, aromatic aldehydes and ammonium acetate at room temperature with excellent yields short reaction time and simple work up. The molecule shows excellent antimicrobial activity.

CHO H O O N R` or CAN, NH4OAc N 0 OH CH3CN, RT R' 3 2 1a 1b Schem-1

The imidazole ring system is of particular interest as a bioactive molecule because it is a component of histidine that produces histamine in metabolic process. The potency and wide applicability of the imidazole pharmacophore can be attributed to its hydrogen bond donor-acceptor capability as well as its high affinity for metals which are present in many protein active sites. Triaryl Imidazoles are used as a photosensitive material in photography. In addition they are of interest because of their antimicrobial activity, herbicidal, analgesic, fungicidal, anti-inflammatory and antithrombotic activities.

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F-6

Effect Of Parthenium Hysterophorus Linn. OnSeed Germination, And Studies The Antimicrobial Activities.

S. Kshirsagar1, V. Bhise1, D. Gaikwad2, P. Solunke2, R. Wakde3*, Dr K. Prashar4

1,2Students in Biotechnology, B.R. Barwale College, Jalna. (431213) 3*Assistant Professor, Department Of Biotechnology, B.R. Barwale College, Jalna. (431213) 4Principal, B.R. Barwale College, Jalna. (431213)

ABSTRACT

Parthenium(Parthenium hysterophorus L.) has become an invasive woody plant in recent times in many part of the world affecting the agricultural productivity.Hence we carried out to evaluate the effect of different part of the plant leachate on seed germination and metal tolerating capacity of the seeds against iron, mercury and nickel during germination. The extracts obtained from aerial parts of P. hysterophorus was evaluated for antimicrobial activity against bacterial species (Escherichia coli) and fungal species (Aspergillus niger, Fusarium oxysporum). In the present study show the importance of weeds can be used to obtain growth factors easily in aqueous medium and that can be utilized for producing new bioactivity compounds having antibacterial and antifungal activity.

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F-7

Medicinal uses of Plants by Indigenous people of Mahu Taluka, Nanded District, Maharashtra, India.

Vijigiri Dinesh G.

Department of Botany Shri Renukadevi Arts, Commerce and Science Mahavidyalaya, Mahur, Dist: Nanded, (M. S.)

ABSTRACT

The present Medicinal uses of plants by indigenous people of Mahur Taluka resulted in the information on the plants used in treating many diseases. For which about 41 plants species belonging to 40 Genus and 28 families are used. Of these, maximum species belongs to Fabaceae with 5, Verbaniaceae with 4 species, Caesalpiniaceae and Apocynaceae with 3 species. Information gathered from Mahur taluka indicates that the indigenous and other village people of this region have good knowledge of plants in treating different ailments, but their continuous and progressive exposure to modernization may result in extinction of the rich heritage of knowledge in the course of time. Majority of preparation are from Leaves and Stem bark. Method of preparation of medicine and its application. Among the plant parts used in different formulations, Leaves are profusely used which is followed by Leaves, Stem, Seed, Root and fruit.

Keywords: Mahur, Medicinal Plants, Indigenous.

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F-8

An efficient L-proline promoted synthesis and antimicrobial study of 2,5- disubstituted-1,3,4-oxadiazoles

A.V. Nakhate1*, S. V. Shinde2 1Marathwada Institute of Technology, Aurangabad – 431010. 2Pratibha Niketan College, Nanded – 431604

ABSTRACT The carbon compound which contains nitrogen atom in their cyclic rings attains significant importance because of their prevalence in natural products. Also, due to their various pharmacological properties. They also possesses optoelectronic and photochemical properties. In particularly, heterocycle of five membered rings are of great interest due to its inherent biological activity. Among all, 2,5-disubstituted-1,3,5-oxadiazoles are the privileged scaffolds in different areas of material science, polymer, pesticidal and medicinal. Oxadiazole and its derivatives act as an anticancer, benzodiazepine receptor agonists, analgesic, antimicrobial, diuretic and tyrosinase inhibitos. Many derivatives of oxadiazoles are in late stage clinical trials including zibotental and furamizole. On the other side, raltegravir, an antiretroviral drug for the treatment of HIV infection, has been launched into the pharmaceutical world market nowadays. We have developed a simple and highly efficient method for a two component condensation of aromatic aldehyde and acyl hydrazide with L-proline (10 mol%) as catalyst in ethanol (20 mL), was stirred at reflux temperature. The significant advantages existing by this method are inexpensive and readily available catalyst, simple procedure and high yield of products. The derivatives are tested for its antibacterial and antifungal properties.

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F-9

SYNTHESIS OF 1H-INDAZOLES USING LEMON PEEL POWDER AS A NATURAL, GREEN AND EFFICIENT CATALYST UNDER ULTRASOUND IRRADIATION

Sunil U. Tekale, Satish U. Deshmukh, Ajit K. Dhas and Rajendra P. Pawar

Department of Chemistry, Deogiri College, Station Road, Aurangabad - 431 005 (Maharashtra), India

ABSTRACT A series of bioactive 1H-indazoles was synthesized from 2-substituted aromatic aldehydes and hydrazine hydrate using lemon peel powder as a green and efficient natural catalyst. The reported method affords good yield under ultrasonic irradiation.

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F-10

Base catalysed one pot green synthesis of Tetrahydrobenzo[b]pyran

Vijay P. Pagore Department of Chemistry, Shri Muktanand college, Gangapur, District-Aurangabad, Maharashtra

ABSTRACT

Base catalyzed green protocol has been developed for one-pot three-component condensation of aldehydes, malononitrile and dimedone for the synthesis of biologically important moiety tetrahydrobenzo[b]pyran derivatives. The reaction afford moderate to excellent yield of products. Benzopyrans are basic backbone of various types of polyphenols and widely found in natural alkaloids, tocopherols, flavonoids, and anthocyanins. These compounds are also industrially important as cosmetics, pigments and potentially biodegradable agrochemicals.

R O CN

O O O NH2 (1) (2) (3)

Department of Botany, Deogiri College, Aurangabad. Page 135

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F-11

Green Synthesis of Pyran Derivatives Using Lemon Peel Powder as a Natural Catalyst and their Antimicrobial Activity R. D. Ingle, C. S. Patil, S. R. Bembalkar, M. V. Katariya and R. P. Pawar Department of Chemistry, Deogiri College, Station Road, Aurangabad - 431 005 (Maharashtra), India

ABSTRACT A green and efficient protocol for the synthesis of bioactive pyran annulated heterocycliccompounds at room temperature in ethanol has been developed using lemon peel powderas a catalyst. This method provides operational simplicity and environmentally benign green approach.

Keywords: Lemon peel, Green synthesis, Pyran, Antimicrobial etc

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F-12

Ethanobotanical Significance of some Medicinal plants from family Cucurbitaceae and Asclepiadaceae

Shweta Kamble, Shamal Mali, Dnyaneshwar Mane and B.M. Waghmare Botany Research Centre, Maharashtra Mahavidyalaya, Nilanga. Dist. Latur (MS), India.

ABSTRACT ‘Ethanobotany’ and it is defined as, a totally natural and traditional relationship and an interaction between man and his surroundings. It is applied to the natural and direct relationship with plant and people at any level of antiquity, primitiveness. It is clear from the literature, the numerous medicinal plants were found in Kinwat Forest. Therefore, Ethano- medico-botanical studies of medicinal plants of family Cucurbitaceae and Asclepiadaceae were carried out with their ethono-medical importance. Therefore, the four members such as, Citrullus colocynthis, Corallocarpus epigaeus, Diplocyclos palmatus, Trichosanthes tricuspidata of family Cucurbitaceae and the two members of the family Asclepiadaceae such as, Ceropegia bulbosa, Pergularia daemia has been selected for the study of their practical application.

Citrullus colocynthisseed powder show antidiabetic property. Fruit and fruit pulp is used in constipation, fever, worms, stomach ache, elephantiasis, dyspepsia, ulcer, asthma and bronchitis. Roots are used in malarial fever.Corallocorpus epigaeus tubers are used as medicine in snake bite, in rheumatism and dysentery. Dried seeds of Diplocyclos palmatus are found to be induced pregnancy. Seed powder given to increase ovulation. Plant is a tonic, aphrodisiac, thermo genic, anti-inflammatory. Various parts are used in headache, paralysis of tongue, colic pains, constipation, snake bite and increase the sperm quality. Trichosanthes tricuspidata root paste is useful in diarrhoea and diabetics also used in acidity and stomach complaints. Roots are cathartic cures bronchitis, headache. The whole plant is purgative, laxative, cooling, digestive, anthelminthic, carminative, emetic and antipyretic. The fruits are useful in skin diseases, burning sensation, leprosy, ulcers and general weakness. Ceropegia bulbosa tubers are sweet and used as an energetic tonic, digestive and aphrodisiac. It cures cold and eye diseases. Pergularia daemia latex is applied on the affected portion for fifteen days found to be regenerating the hairs. Leaves have anti-inflammatory properties. It is use in gynaecological problem.

Keywords: Ethanobotany, Kinwat Forest, Cucurbitaceae,Asclepiadaceae

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F-13

BioprospectingPentapteras of Genus: Terminalia L. of Maharashtra state, India for Antioxidant activity.

Salve, A. P. & Dhabe, A. S.

Dept. of Botany, DoshiVakil Arts & G. C. U. B. Science & Commerce College, Goregaon, Raigad; [email protected] Dept. of Botany, Dr. B. A. M. U., Aurangabad; [email protected]

ABSTRACT In India, perception of “Bioprospecting” is having its fundamentals from Ayurveda as Traditional Ethnobotanical Knowledge (TEK) that fuels bioprospecting. Focusing this Petaptera’s (Section: TerminaliaRoxb.) of Genus: Terminalia L., collected from Maharashtra state, India, were Bioprospected for Antioxidant activity. Taxonomically, TerminaliaalataRoth.,Terminaliaarjuna(Roxb. Ex DC.) Wight &Arn. andTerminaliatomentosa Wight &Arn. constituted to the Section: PentapteraRoxb. of Genus: Terminalia L. and were experimented for 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Free Radical Scavenging Activity (FRSA) by Blois method & Nitric Oxide (NO) FRSA by

Nims et al. method for calculating Half maximum Effective Concentration (EC50) and its inverse called as Antiradical Power (ARP). All accessed FRSA for Terminaliaspp. obeyed Concentration dependent expression following Non- linear progression for Ethanolic Bark extracts (EBE)- DPPH absorbance (at 517 nm), EBE- NO absorbance (at 540 nm), quantal concentration dependent % DPPH scavenging curves and concentration dependent % NO scavenging curves, recorded through UV- Vis dual beam Spectrophotometer. These results were expressed as % Free Radical Scavenged (% FRS) for three replicates (n= 3) and their values were expressed as mean ± S. D. (standard deviation). These concentration dependent radical scavenging curves were processed using Origin Pro 8.5 Keywords: Genus: Terminalia L., PentapteraRoxb., Antioxidant activity, Ethanolic Bark extracts (EBE), 2,2-Diphenyl-1-picrylhydrazyl (DPPH) Free Radical Scavenging Activity

(FRSA), Nitric Oxide (NO), Half maximum Effective Concentration (EC50) and Antiradical Power (ARP).

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F-14 STUDY OF ETHENOBOTANICAL PLANT'S USED ON CALCULUS (Kidney and Galbladder stone problems)

Ladda R.G.

ACS College Gangakhed.

ABSTRACT

A stone in the kidney (or lower down in the urinary tract). Also called a kidney stone. The stones themselves are called renal caluli. The word "calculus" (plural: calculi) is the Latin word for pebble. Renal stones are a common cause of blood in the urine and pain in the abdomen, flank, or groin. Kidney stones occur in 1 in 20 people at some time in their life. The development of the stones is related to decreased urine volume or increased excretion of stone-forming components such as calcium, oxalate, urate, cystine, xanthine, and phosphate. The stones form in the urine collecting area (the pelvis) of the kidney and may range in size from tiny to staghorn stones the size of the renal pelvis itself.In present paper local traditional medicinal plants which are used as anti-calculus are collected by collecting information from folk peoples, traditional practitioners and arranged alphabetical order with botanical name, family and parts used.

Keywords : Calculus, traditional medicinal plants.

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Theme - H Microbiology and Aerobiology

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H-1

Aerobiology in the transmission of Infectious Diseases through Droplet air Infection.

Dr.Sanjay A. Kamble1*, Dr. M.S. Kharate2

1* Department of Botany, Vinayakrao Patil College Vaijapur-423701, Dist. Aurangabad, Maharashtra, India

ABSTRACT

The branch of Pathology, Aerobiology plays a fundamental role in the transmission of infectious diseases. As infectious disease and infection control practitioners continue employing contemporary techniques. It deals with the computational fluid dynamics to study particle flow, PCR methodologies to quantify particle concentrations in various settings, and epidemiology to track the spread of disease), the central parameters and variables affecting the airborne transmission of pathogens are becoming better known. The study reviews many of these aerobiological parameters (e.g., particle size, particle type, the duration that particles can remain airborne, the distance that particles can travel, and meteorological and environmental factors), as well as the common origins of these infectious particles, through transmission of infectious diseases through droplet air infection.

Keywords: Aerobiology, Droplets, Air Infection, Particle Count.

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H-2

Determination of Potential Allergenic Fungal flora and its

Clinical Reflection in Urban and Rural Areas of of Maharashtra

R.M. Kadam Assistant Professor and Head Dept. of Botany, Mahatma Gandhi Mahavidyalaya, Ahmedpur

ABSTRACT

Aerobiology is a science, which deals with the source of organisms or the other materials and their release into the atmosphere, transport, deposition and their impact on various kinds of living beings like plants, animals and human beings. Such studies are also known as "microbiology of the atmosphere".Increased awareness of environmental biopollution has led to the monitoring and studying the aspect of biopollution. Both at national and international levels health problems like allergy, asthma, pulmosis, rhinitis, pulmonary disorders, and skin diseases prove the important of study of bioarosoles. Importance of aerobiology in plant pathology and human health hazards is well recognized.The present investigation is carried out to know the concentration of allergenic fungal spores in the atmosphere and to correlate fungal spore concentration and allergic diseases.The aerobiological investigations records 58 types,out of these, 3 belonging to Phycomycetes, 13 to Ascomycetes, 4 to Basidiomycetes, 33 to Deuteromycetes and 5 to other types. Deuteromycetes was the dominant group in both seasons. Its contribution in first and second was 67.49% and 74.21% respectively, followed by Basidiomycetes (14.57% and 11.77%), Other types (9.39% and 6.99%), Ascomycetes (6.87% and 5.02%) and Phycomycetes (1.65% and 1.98%). The present investigation would be useful for evolving and efficient mechanism to analyze the allergic diseases caused by the fungal spores in different seasons.

Department of Botany, Deogiri College, Aurangabad. Page 142

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H-3

Indoor Air Quality and Health Impacts

V.S. Nagpurne Dept. of Botany, Maharashtra Udayagiri Mahavidyalaya, Udgir

ABSTRACT

It is generally recognized that Indians spend 70% or more of their time indoors. Despite this, relatively little research has been done on the quality of air in our homes, schools, recreational buildings, restaurants, public buildings, offices, or inside vehicles. Indoor air quality can be defined as the totality of attributes of indoor air that affect a person's health and well-being.Common health problems that result from exposure to poor indoor air quality include: sensory and skin irritation; neurotoxic symptoms; hypersensitivity and odour and taste symptoms.In the present study, in order to determine mycoflora, indoor air samples were taken in twenty buildings located in the Udgir city within a 1-year period between January 2018 to December 2018. Samples fromdifferent locations from the corridors, rooms of buildings in all were taken for each period. Within the period of the study, in indoor air samples, 245 microfungus colonies were counted. Thirty two species were identified that belong to 14 genera as a result of the identification of isolates obtained. We found a significant relationship between dampness and high fungal spore count. The study revealed high prevalence of predominantly allergenic fungal spores in the indoor environment.

Keywords: Indoor Air quality, Mycoflora.

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ANTIBACTERIAL ACITIVITY OF SOME FRUIT EXTRACT AGAINST Xanthomonas tagetes Rangaswami and Sanne Gowda

Sanghamitra V. Arak and R.J. Sawant

Research centre in Botany Shri Muktanand College, Gangapur -431109

ABSTRACT

The bacterial leaf spot was caused by pathogen Xanthomonas tagetes Rangaswami and Sanne Gowda It is one of the disease of Tagetes erecta L. The pathogen mainly attacks on leaves of the plant and yellow brownish spots appear on leaf. In present investigation the antibacterial activity of some fruit extracts were tested against this pathogen. Fruits also have different antimicrobial activities. Five Fruit crude extract namely Acacia nilotica L.,Capsicum annum L., Coriandrum sativum L. Foeniculum vulgare Mill. and Trachysperm amni,(L) Spargue were used for the experiment. Coriandrum sativum L. has given excellent result in experiments. The experiments has been done by using cup-plate method to examine the antibacterial activity. This study will be helpful for effective biological control of bacterial leaf spot disease of Tagetes erecta.

Keywords: Antibacterial activity, fruit extract, Xanthomonas tagetes Rangaswami and Sanne Gowda

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The Role Of Epicoccum as a biopollutant regarding asthma

S. W. Bhivgade, S.N. Sangekar and U.S. Salve* Department of Botany , Yogeshwari mahavidyalaya , Ambajogai (M.S.) *Department of Botany ,Swa. Sawarkar mahavidyalaya , Ambajogai (M.S.)

ABSTRACT

Human beings are known to suffer from major allergens such as fungal spores, pollen grains and house dust present in the atmosphere. Inhalation of air borne fungal spores is the main causative factor for respiratory allergic diseases in human beings.Indoor survey of aeromycoflora was performed by using Roto Rod air sampler ,petriplate exposure and eosinophil count of asthmatic patient in Ambajogai.The highest concentration of Epicoccum spores was recorded in the house of patient . ‘F’ (3.02%) in the month of Feb2002.Epicoccum spores are potential sensitizes in human being and their prevalence is important for diagnosis and environmental management of asthma .

Key Words:Epicoccum, asthma, eosinophill count, Aeromycoflora.

Department of Botany, Deogiri College, Aurangabad. Page 145

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H-6

AEROMYCOFLORA OVER SUNFLOWER FIELD

G.M. Pathare and P.S.AnarseAnandrao Dhonde Alias Babaji College Kada Amolak Science College Kada Tal – Ashti , Dist- Beed (M.S) India

ABSTRACT

Present paper deals with aero mycological investigation were carried out by using Volumetric Continuous Tilak Air Sampler over Sunflower fields at Kada for two different seasons Kharif and Rabi seasons of the years 2003and 2004.in Kharif seasons 58 and in Rabi seasons 67air borne components were trapped and identified .All the trapped air borne mycoflora types have been categorized under Zygomycotinia ,Ascomycotina ,Basidiomycotina and Deutromycotina . Hypel fragments ,Insect parts ,Pollen grains and Protozoan cyst were included under Other types .In kharif seasons Cladosporium dominated the airspora composition and contributed 26.02% ,followed by Alternaria 13.75% ,Rust spore 7.79% ,Curvularia 6.20%, etc .In rabi season also Cladosporium dominated the airspora composition and contributed 24.62% followed by Alternaria 7.00%, Periconia 6.85%, Smut 6.00%, Hypel fragments etc.

Keywords:Airsampler, Airspora ,Mycoflora ,Fungal diversity.

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Theme I Physiology and Biochemistry

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The Quantitative Measurement of Leaf Chlorophylls and Carotenoids from some leguminous fodder crops by Spectrophotometry

Rajput R. D., Solanke S. N*.and Patil R. P Department of Botany, Shri Muktanand College, Gangapur (M.S.)

ABSTRACT The present work is carried out on the basis of comparison of extraction of leaf chlorophylls viz. Chlorophyll-a, Chlorophyll-b and total chlorophyll and Carotenoids from cultivated some leguminous fodder crops by using 80% Acetone as extracting method (Arnon, 1949) and the study is concerned with the concentrations of leaf pigments found between the treated and control leguminous fodder crops.

Keywords:Quantitative Measurement, Spectrophotometry, Chlorophylls, Carotenoids, Leguminous Fodder crops, Extraction, Solvent.

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CHANGES IN CORIANDER (Coriandrum sativum L.) LEAF JUICE DURING STORAGE WITH REFERENCE TO THE BIOCHEMICALS

U. S. Salve

Department of Botany, Swa. Sawarkar Mahavidyalaya, Beed-431122 (M. S.)

ABSTRACT

In India, leaves of coriander (Coriandrum sativum L.) are used frequently in curries. It is cultivated in almost all the states as an important subsidiary crop. The tender, stem, leaves and flowers of this crop have a pleasant aromatic odour and are the richest source of vitamin A and C. Attempts have been made during present investigation to point out biochemical changes associated with the storage of leaf juice upto 48 hours. Leaf juice was extracted from green foliages of Coriander (Coriandrum sativum L.) and stored for 48 hours. Studies on biochemical changes during storage revealed that the dry matter (DM), Ph, nitrogen (N) content and chlorophyll content in the juice gradually decreased and increase in the amount of lactic acid is observed when the juice was stored upto 48 hours.

Key Words: Coriander, Leaf Juice, Biochemical changes, Lactic Acid, Chlorophyll content.

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EFFECT OF ELECTRICITY ON SEED GERMINATION OF CERTAIN CROP PLANTS

R. U. Bhagyawant and Mukundraj B. Patil*

*Department of Botany, Late Ramesh Warpudkar Arts, Commerce and Science college, Sonpeth, Dist. Parbhani

ABSTRACT:

Experiments were carried out to study effect of electricity on seed germination of(Wheat) Triticum aestivum, (Carrot) Daucus carota and (Groundnut) Arachis hypogeal. For this 12 pots were taken for each seed type and grouped into four sets each containing three pots. Twenty seeds of Triticum aestivum, and Arachis hypogea and 25 seeds of Daucus carota were used for sowing in each pot of respective set. While keeping all the parameters constant 3V, 6V and 9V electricity was supplied for 10 min daily to the experimental Pots while three (control) was not supplied with electricity. Number of seedling emerged out of soil where counted and percentage of germination was calculated. In Groundnut highest percentage of germination (90%) was recorded in pots supplied with 6V electricity followed by pots receiving 3V electricity which shows 80% seed germination. In wheat highest percentage of germination was recorded in pots receiving 3volt as well as 6volt electricity. In Daucus carota highest percentage of germination (77%) was recorded in pots receiving 3volt electricity followed by (76%) in pots receiving 6volt electricity. From this experiment it was concluded that seeds germinated under electric stimulus (3V and 6V) shows highest seed germination in all three crops but it was decreased with increased intensity (9V).

Key words: Electricity, Percentage germination, Wheat, Carrot, Groundnut etc.

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I-4

Biochemical Studies in Hybanthus enneaspermus (L.) F. Muell.

Medicinally Important Plant.

Prashant Patankar and *Sanjay R. Biradar *Head, Department of Botany, B.S.S.A.S.C. College, Makni-413604 Dist. Osmanabad, [M.S.] India.

ABSTRACT:

Hybanthus enneaspermus (L.) F. Muell.(Violaceae) is one of the important plant used in the treatment of anemia and has no toxicity as per earlier reports of fertility. Plant is used in the treatment of different kinds of diseases such as acute renal injury, diabetic complications, analgesic and anti-allergic, anti-proliferative and antioxidant etc. The plant also showed biologically active biochemical macro and micro elements.Present study were carried out for the elemental analysis of different parts of Hybanthus enneaspermus such as root, stem and leaves. The experiment were carried out to analyze 15 different elements viz., Total Nitrogen (N), Total Phosphorous (P), Total Potassium (K), Calcium (Ca), Magnesium

(Mg), Sodium (Na), Protein, Sulphur (SO4), Copper (Cu), Iron (Fe), Zinc (Zn), Manganese (Mn), Boron (B) and Aluminum (Al). Results of present study revealed that Hybanthus enneaspermus is a rich source of nutritional elements.

Key words: Hybanthus enneaspermus, biochemical, Protein, nitrogen

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EFFECT OF CHEMICAL PRE-TREATMENTS ON SEED GERMINATION AND SEEDLING GROWTH OF SYZYGIUM CUMUNI (L.) SKEELS.

Rajesh Shrirangrao Gaikwad Department of Botany, Swami Vivekanand Senior College, Mantha- 431504. Dist. Jalna (Maharashtra) India.

ABSTRACT

The present study was carried out to investigate the influence of different growth regulators (IAA+ IBA and IAA+ NAA) on seed germination and seedling growth of Syzygium cumuni. It was observed that IAA + IBA at 600 ppm favoured maximum seed germination. On the other hand shoot length and root length was maximum at IAA+ NAA at 400 ppm .

Key words:Syzygium cumuni , germination, shoot length and root length .

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Ultrasound Induced Germination of Seeds

Shirsat T.T, Jondhale M.B., Kulkarni S.G Department of Biophysics, Govt. Institute of Science, Aurangabad-431004 MS

ABSTRACT

The uses of ultrasound in scientific and medical fields have been revolutionary owing to its beneficial effects. The present study explores the ultrasound exposure induced alterations in the germination of the moth bean seeds. In the present research work ultrasound exposure to moth beans seeds(two sets dry seeds and wet seeds ) were exposed to different frequencies (5MHz, 7MHz, 9MHz) & duration (5min,3min) at 8oc.The overall growth characteristics of the exposed seeds as compared to control seeds were monitored to calculate the germination percentage. The germination was stimulated by ultrasound depending on frequency and time of exposure.

Key words: Ultrasound, Seed germination

Department of Botany, Deogiri College, Aurangabad. Page 153

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Efficiency of Phytohornes on Vigour Index of Vignaradiata (L.) Wilczek

Var.

Lata Dattu Done and Avinash Ade

Department of Botany, Arts, Science and Commerce College, NaldurgTq. Tuljapur Dist. Osmanabad – 413602 (M. S.) India.Department of Botany, Savitribai Phule University, Pune-411007 (M .S.) India. ABSTRACT

Vignaradiata (L.) Wilczek Var. belong to family Leguminaceae, sub- family, papilionaceae. In Maharashtra, the green gram grown as a pulse crop. It is one of the most ancient legumes of India and is still an important crop. It rich in protein and commonly called as ‘dal’. The response on seed germination of Vignaradiata (L.) Wilczek Var. was investigated on IAA, GA, SA and 2, 4-D, in different concentrations viz., control (000) 500, 1000, 1500 and 2000 ppm respectively. The higher percentage of seed germination was occur in SA in high concentration at 1500ppm (90%) followed by IAA (80%). The plant length and root length were significantly increased in all concentrations of IAA, GA, and SA. 2,4-D did not show any significant difference. The shoot length was moderate in all hormones except GA. The number of leaves, fresh weight and biomass did not variable as compared to control. The result showed that SA and IAA increased seed germination, plant length and root length. As cell elongation hormone the GA showed increased in plant length, shoot length and root length.

Key words: Vignaradiata (L) Wilczek Var.,hormone, IAA, GA, SA, 2, 4-D.

Department of Botany, Deogiri College, Aurangabad. Page 154

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I-8

PREPARATIONS OF VERMIN WASH ITS EFFECTS ON PLANT GROWTH AND DEVLOPMENT OF RED SPINACH (Amaranthus dubius)

D. Rajesh 1 2 and Dr. Ahmed Abdul Haleem Khan 1*

1. Department of Botany Telangana University, Nizamabad 2. Department of Botany, GDC-Bichkunda, , Telangana state.

ABSTRACT

The present paper focused on the leafy vegetable species. For the growth and development of red spinach in India. Vermin wash is good organic manure as a source of almost all the essential nutrients is an important factor on crop growth. Vermin wash plays important role in the plant growth and development when applied to soil and spread foliage. It is excellent growth promoter as bio-pesticides in recent days the vermin wash is used as liquid manure. The increasing use of chemical fertilizers to grow vegetables such as red spinach has caused numerous problems related to the environment and human health. We are prepared two concentration of vermin wash, like 25% concentration and 50% concentrations. Experiment through pot culture and study was conducted to find out the effect of vermin wash on red spinach plant. In pots we used red soil. After 15 days of germination spraying was done. With following concentrations 25%, 50% and with out vermin wash (controlled). The parameters such as plant height, shoot length, root length, number of branches, number of leaves, leaf length and breadth of leaf where measured. Pot culture studies were carried out to find out the effect of vermin wash spray on the growth and development of red spinach. Red spinach growing was calculated and observed under all treatments (15, 20, 25) days. Red spinach were grown significantly higher when compared to control group of plants. The maximum growth of leaves and plants found with the spray concentration of 25% vermin wash sprayed on 15, 20, 25 days after germination. In 50% concentration plant growth inhibits. The growth of control plants (without spray of vermin wash) found to be poor. So vermin wash normal concentrations increase the plant growth and development. Key words: Amaranthus dubius,vermin wash, growth and development , pot culture.

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Theme J

Phyto chemistry Phytogeography and Bio- physics

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ANTIBACTERIAL, ANTIOXIDANT AND PHYTOCHEMICAL ANALYSIS OF RIPE AND UNRIPE BANANA PEEL

*Vaishali Dange and 1Anruni Mohanty *Assistant Professor, Institute of Biosciences and Technology, M.G.M. University, Aurangabad 1PG Student, Institute of Biosciences and Technology, M.G.M. University, Aurangabad

ABSTRACT The banana peel processing was optimized by drying temperature. The extract was obtained by different solvent with respect to time for extraction. The drying process was done at room temperature for several weeks. The ground banana peel samples were extracted with different solvents viz. 80% ethanol, 70% acetone and distilled water. The extraction was performed by using magnetic stirrer for 30 minutes. Phenolic content was found highest in aqueous ripe sample with concentration of 84μg/ml and lowest in ethanol unripe with 13μg/ml. Disc diffusion method used to verify the existence of antimicrobial activities on different microbial isolates like E. coli, S. typhi, Salmonella Shigella, E. faecalis, P. fluorescens, and B. subtillis, Staph all. Results were obtained in acetone extract against P. fluorescens with zone of clearance having 27mm diameter whereas less zone of clearance was observed in ethanol extract against E. faecalis .with diameter of 11mm.Thin Layer Chromatography is used to determine the alkaloids and vitamin (E, C) in different extracts, Vit. C and tannic acid were spotted followed by high Performance Liquid Chromatography that is used to determine the bioactive compounds in banana peel extract. Salicyclic acid was detected in both the ripe and unripe aqueous samples using HPLC technique.

KEYWORDS: Antioxidant, Antibacterial, phytochemical, Banana peel, TLC, HPLC.

Department of Botany, Deogiri College, Aurangabad. Page 159

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Detailed quantification of base metals and precious metals of scrap PCB of mobile phones by FE SEM/EDAX and Inductively Coupled Plasma-Optical Emission Spectroscopy method.

1Sonawane P.M and 2Gupta S.G.

1Department of Biotechnology, Government Institute of Science, Aurangabad. 2 Director, Government Institute of Science, Aurangabad.

ABSTRACT

With advances in mobile technology, the obsession for the latest mobile gadgets is increasing which is further acting as a major contributor towards the generation of e-waste. So, the current study was focused to UNLOCK the value of metal content of scrap mobile printed circuit boards (PCB). For this purpose, the investigative study was done using Field Emission Scanning Electron Microscopy (FESEM) to analyse the topology of 0.5 mm particle size of Electronic Scrap Material (ESM) of PCB of scrap mobile phones and Energy Dispersive X-ray Analysis Spectroscopy (EDAX) was done to assure the presence of base and precious metals in it. Later, the confirmatory study was more focused on the determination of multi-element content by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) method. ICP OES method which is a highly sensitive and accurate method was done using 0.5 mm fraction of PCB of mobile phones. The study revealed, the presence of base metals Co < Mg < Pb < Zn < Ni < Al < Cu and precious metals Ag < Pt < Au. The analysis showed among base metals Cu to be present in highest concentration i.e. 244.303 g/ Kg and gold to be present as 1106.6 mg/Kg.

Keywords: E-waste; Printed Circuit Boards; FE SEM; Inductively Coupled Plasma; EDAX.

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J-2

PHYTOCHEMICAL PROFILE OF CURCUMA INODORA Blatt. RHIZOME EXTRACT

Anand S. Jadhao1 and Anil S. Bhuktar2

1 Department of Botany, B. S. Patel Arts, Commerce and Science College, Pimpalgoan Kale, Ta. Jalgoan (Jamod), Dist. Buldhana, Maharashtra (India). 2 Department of Botany, Vivekanand Arts, Sardar Dalipsingh Commerce and Science College, Samarth Nagar, Aurangabad, Maharashtra - 431 001 (India)

ABSTRACT Curcuma inodora Blatt. (Zingiberaceae), rhizome used in the treatment of Psychosomatic disorders, constipation, in muscular pain, also have anti-inflammatory, antipyretic and wound healing activity. Methanolic rhizome extracts screened for detection of phytochemical constituents by using HRLC-MS technique. Metabolites analysis by ESI-Q- TOF-MS revealed presence of 14 major compounds namely 1alpha,25-dihydroxy-26,27- dimethyl-20,21,22,22,23,23-hexadehydro-24a- homovitamin D3, Trp Gln Trp, N-(1-methyl- 2-hydroxy-2-phenyl-ethyl) arachidonyl amine, Arg Lys Ile, Methylprednisolone succinate, (6R)-vitaminD3 6,19-(4-phenyl-1,2,4-triazoline-3,5-dione)adduct, Prednicarbate, N- Palmitoyl-L-serine phosphoric acid, 1-(9Z,12Z,15Z-octadecatrienoyl)-2- (4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol, enzonatate(2,5,8,11,14,17,20,23,26- Nona oxaoctacosan-28-ylp-(butylamino)benzoate, (17E)-1alpha,25-dihydroxy- 26,27- dimethyl - 17, 20,22,22,23,23-hexade hydro -24a- homovitamin D3, Salmeterol, 1,2- ditetradecanoyl-sn- glycero-3-phospho-(1'-sn- glycerol) and Digitoxin. This report is the first of its kind to analyze chemical constituents of Curcuma inodora using HR-MS. In addition to this, results of HRMS profile can be used as pharmacognostical tool for identification of Curcuma species. Key words: Curcuma inodora, Phytochemical,HRLC-MS

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SEASONAL VARIATIONS OF PHYTOCHEMICAL VALUES OF AEGLE MARMELOS

V. B. Kadam

P.G. Department of Botany & Research Centre, K.T.H.M. College, Nasik – 422002

ABSTRACT

The seasonal variation of total ash, acid soluble ash and acid insoluble ash have been investigated leaves , wood, bark and root of Aegle marmelos , which are medicinally important.Comparative account of total ash, acid soluble ash and acid insoluble ash content of bark showed high level of total ash in summer season (14.900 %) and low level of total ash content in root during monsoon season(6.700 %) . The water soluble ash showed higher level in bark during (5.475 %) and lower in root of monsoon (2.100 %). Comparative account of water insoluble ashin leaves showed higher (11.125 %) in monsoon and lower in the root during monsoon( 4.600 %) as compare to other seasons.

Key Words: Total ash, water soluble ash, water insoluble ash, Aegle marmelos

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J-4

‘HPTLC’ an important tool for quantification of herbal product: A case study

Sutare M S, Department of Botany Yashwantrao Patil Science College, Solankur Tal. Radhanagari Dist. , Shivaji University Kolhapur

ABSTRACT

Large number of population prefer the Ayurvedic or herbal medicines to treat the various ailments. Herbal medicines are available in three forms as raw plant material or processed plant material or medicinal herbal product but mixtures have several chemical components. Therefore, it is very important to know the specific component having potential for treatment. High-performance thin layer chromatography (HPTLC) is one of the sophisticated instrumental techniques for qualitative and quantitative analysis of the herbs and herbal drugs. Hence, a case study has performed to quantify vasicinone component from Adhatoda zeylanica.

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J-5

Histochemical Investigation of Madhuca indica Gmel S.S.Tambe*, V.B.Kadam

*Department of Botany Arts, Science and Commerce College ManmadKTHM College Nashik

ABSTRACT

The present work is under taken with a view to analyze, similarities and dissimilarities in morphological, anatomical, microscopically, physicochemical. These plants are commonly available and medicinally useful in this geographical area and this study would form a foundation for understanding the pharmacological and therapeutically effectiveness of these varieties. One of such resources is folk medicines. Systematic screening of them may result in the discovery of novel effective compounds. This Research Article Histochemical investigation of Madhuca longifolia these plants have many folk medicinal used.

KEY WORDS: Hisochemistry, Folk Medicine, Madhuca indica

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Evaluation of Antibacterial Activity of Leaf Extract of Jasminum Officinale Against Oral Pathogens in Ulcer Treatment

Sopan A. Shinde and Vanita U. Pochhi Shri Shivaji Science and Arts College Chikhli, Dist Buldana, 443201 (MH)

ABSTRACT

Medicinal Plants are endowed with phytochemicals that are vital to counter various metabolic disorders like Oxidative damage in cells causing various degenerative diseases. Hence, the present study deals with the assessment of antioxidant activity and phytochemical screening of the aqueous extract of Jasminum officinale leaves. From ancient times, plants have been used in traditional medicines for treatment of different ailments. Medicinal plants is one of the richest bio resources for traditional and folk medicines till date. Jasmine is botanically known as Jasminum officinale or Jasmininie and belongs to the olive family of Oleaceae. Literature report suggest that Jasmine is analgesic, antidepressant, antiseptic, expectorant, aphrodisiac, sedative, stomachic, diuretic, depurative, astringent, stimulating, anti-oxidizing, anthelmintic and anti-inflammatory in nature. The objective was to study antibacterial activity of Jasminum officinale extracts against mouth ulcer causing organisms. The antibacterial activity has been studied against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis & Enterococcus faecalis by agar well diffusion method. Leaves extract of J. officinale give effective results against oral pathogens causing mouth ulcer. Acetone and Ethanol extracts displayed a good antibacterial activity. The phytochemical studies revealed presence of Carbohydrates, Proteins, Steroids, Alkaloids, Flavonoids, Phenols, Saponins, Glycosides and Tannins. J. officinale may prove to be effective medicine for the treatment of ulcer

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J-7

A Study of Phyto-Alkaloid and their therapeutic uses in treating neurological problems with certain Homeopathic Medicines

Dr. P. Latha

Department of Botany, Girraj Government College (A), Nizamabad, T.S. ABSTRACT

The chemical substances obtained from plants are called phyto-chemicals, the natural substances and plant products. Because of these substances and products of plants, medicinal value of the plant is derived due to their therapeutic effect on various parts of the human body after consumption. The various phyto-chemicals in plant are classified into groups such as Carbohydrates, Lipids, Alkaloids, Glycosides, Tannins and Tannic acid, Oils, Resins, Gums and Vitamins. The Alkaloids are bitter-tasting and often toxic substances. These are classified based on their different modes of action as drugs in homeopathic medicine. This paper studies the uses of Homeopathic medicines, Aconitum napellus, Atropa Belladona, Hyoscyamus niger and Conium maculatum in treating the neurological problems due to their alkaloid substances in plants.

Keywords: Phyto-chemicals, Alkaloids, Homeopathy, Neurological.

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J-8

Quantitative investigation of phytoconstituents present in Albizia julibrissin Durazz.methanolic leaf and bark extract.

Wankhade, M.S.

Department of Botany, Sunderrao Solanke Mahavidyalaya, Majalgaon.Dist.-Beed (MS).431131.

ABSTRACT

Albizia julibrissin Durazz.isa medium sized unarmed tree, 5-10 m tall, leaves 4-8 cm long found in outer Himalayan part of the India. The plant is traditionally used in Chinese medicine to treat the depression and anxiety. Pod extract of the plant shows antioxidant and antibacterial activity. Bark extract is applied to bruises, ulcers, abscesses, boils, hemorrhoids and fractures, and has displayed cytotoxic activity. Bark and root water extract showed anticancerous activity. Present investigation was designed for quantitative estimation of bioactive constituents present in Albizia julibrissin Durazz.methanolic leaf and bark extract.The methanolic extracts of the plants leaves and bark were screened for the presence of various phytoconstituents such as steroids, alkaloids, terpenoids, glycosides, flavonoids and carbohydrates. Quantitative estimation results showsleaves extract has (2.41 mg/g) alkaloid,(0.321 mg/g) carbohydrate,(0.55 µg/ml) protein, (0.55 mg/g) phenols,(0.23 mg/g) flavonoids, (0.97 mg/g)saponins, and (0.092 mg/g) tannins. While bark extract showed (5.32 mg/g) alkaloid, (0.207 mg/g)carbohydrate,(0.95 µg/ml) proteins,(0.68 mg/g) phenols, (0.38 mg/g) flavonoids, (0.53 mg/g) saponins and (0.12 mg/g) tannins.

Key words: Albizia julibrissin Durazz., Quantitative estimation, Phytoconstituents.

Department of Botany, Deogiri College, Aurangabad. Page 167

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J-9

Qualitative analysis and TLC Screening of various Stem extract of medicinally important plant Cissampelos pareira Linn.

Sarika V. Lokhande and *Sanjay R. Biradar

*Head, Department of Botany, B.S.S.A.S.C. College, Makni-413604 Dist. Osmanabad, [M.S.] India.

ABSTRACT

Cissampelos pareira Linn. belongs to the Menispermaceae family is a sub-erect or climbing herb, known as Laghupatha in Indian traditional medicine. A very variable, lofty, slender, dioecious, perennial climber this plant commonly distributed throughout tropical and sub- tropical India Cissampelos pareira contains a group of phytochemicals secondary metabolite. Isoquinoline alkaloids mostly present. The present studied was the qualitative analysis of stem part of plant Cissampelos pareira. In this plant various phytochemical was present. Thin layer chromatography was screening of phytohemical stem part of plant using various mobile phase for the separation of compounds.

Key word: Secondary metabolites, TLC, Mobile phase and Cissampelos pareira.

Department of Botany, Deogiri College, Aurangabad. Page 168

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J-10

Pharmacognostic and preliminary phytochemical evaluation of the bark of dalbergia sissoo roxb. SHAIKH M. D., DHOTRE R. S.,R,P.PATIL & M. A. KARE* Department of Botany, Pratishthan Mahavidyalaya, Paithan, Dist- Aurangabad (M.S.) India

ABSTRACT Dalbergia sissoo DC.is belongs to family Fabaceae. It is known as North Indian rosewood. It is reported to be useful in many conditions like fever, ulcers, digestive disorders, anti diabetic and skin diseases. Bark of the plant is used in Ayurveda system of medicine. They require standardization before it enters into the market. The present study was aimed at Pharmacognostic and preliminary phytochemical evaluation of the bark of Dalbergia sissoo. The pharmacognostic investigations were carried out in terms of organoleptic, microscopic and physical parameters,behaviour of bark powder towards chemical reagents,Physico- chemical Evaluations, Phytochemical screening. The dried bark powder was subjected to cold maceration using hexane, alcohol, petroleum ether, chloroform, benzene, methanol, ethanol and water. The bark powder was subjected to a preliminary phytochemical screening to detect the different chemical principle. The phytochemical evaluation revealed the presence of flavonoids, glycosides, tannins, phenols. Keywords: Dalbergia sissoo, Phamacognosy, Photochemistry.

Department of Botany, Deogiri College, Aurangabad. Page 169

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J-11

ANTIMICROBIAL POTENTIAL AND PHYTOCHEMICAL SCREENING OF LEAVES AND FRUITS OF SOLANUM THORVUM (SWARTZ). A MEDICINALLY IMPORTANT PLANT Ugandhar.T1, Venkateshwarlu.M2, Odelu. G3, Rajendra prasad.B4 and Anitha devi.U5 1Department of Botany, Govt. Degree College Mahabubabad -506101, 2Department of Botany University 3College Kakatiya University W arangal-505006, 3Department of Botany, Govt. Degree College Parkal 4506164, 4Department of Botany, UCS, Saifabad, Osmania University, Hyderabad-500004 5Department of Botany Govt. Degree College Peddapally-505172

Back Ground: The present study designed for antimicrobial potential and phytochemical screening of leaves and Fruits of Solanum torvum (Swartz)belongs to the family Solanaceae it is an Important Medicinal Plant. The plant has been used in the folklore system of medicine for the treatment of Asthma, Diabetes and hypertension. Objectives: To evaluate the antimicrobial potential activity, hydrogen peroxide radicals scavenging activity, reducing power, the total phenolic and flavonoids contents, and antioxidant and antifungal activities of methanol, ethanol and water extracts of leaves and fruits of Solanum thorvum.(Swartz). Methods: Methanol, ethanol and water extracts were evaluated against four Gram positive and Gram negative bacterial isolates (Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiellapneumoniae, Bacillus subtilis) and two fungal strains (Aspergillusfumigatus and Aspergillusflavus). Methanol extract at different concentrations was tested for antimicrobial potential and phytochemicals were determined by using spectrophotometric method. Results: The total phenolic content was (40.859±0.017) mg gallic acid/g in the leaves of L. camara, while the total flavonoids were (53.112±0.199) mg/g dry weight. Methanol leaves and fruits extract of Solanumthorvum.(Swartz) showed maximum antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa and was also effective against other bacterial strains as compared to ethanol and aqueous extracts of leaves and fruits. The methanol leaf extract of Solanumthorvum.(Swartz)exhibited significant inhibition (71%) and (66%) against Aspergillusfumigatus and Aspergillusflavus respectively. Conclusions: The methanol extract of the Solanumthorvum.(Swartz) leaves and fruits effective against selected bacterial and fungal strains. Its phytochemical contents have broad antimicrobial properties and the plant might be a novel source of antimicrobial drug.

Keywords: Methanol, ethanol, Antimicrobial, Phytochemicals Solanumthorvum

Department of Botany, Deogiri College, Aurangabad. Page 170

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J-12

Phytochemical studies of Ipomoea clarkei Hook. f.

S. M. Sangle*, S. V. Dongre and A. S. Dhabe Department of Botany, Rajaram College, Kolhapur Kolhapur – (616 004), Maharashtra, India* Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431001, Maharashtra, India

ABSTRACT

Ipomoea is the chief genus in convolvulaceae family it comprises over 500 species. It is a massive and diverse group of flowering plant with common names containing morning glory, kangkung, bindweed etc.Ipomoea clarkei. is rare annual herb, climber and bears golden-yellow flowers. It is described under endangered category.Present study work carried out by phytochemical studies by using HR-LCMS technique. Leaves of the plants were washed with distilled water and dried under shade and grinded with the help of mortar and pestle into a fine powder. Powdered sample were run for Soxhlet extraction for 76 hours and extracted material was to chromatographic HRLC- MS techniques. Analysis of Ipomea leaves shows the presence of secondary metabolite which belongs to a class of Fatty acid, Sterioisomer, Carbaxomide, Metabolite, Fatty acid, Anti ulcer drug, Glycoside, Enzyme, Pigment xanthonoid, Glucuronide, Metabolite. The major and minor compounds are 2,15- dihydroxy-pentadecylic acid, Monoethylglycylxylidide 2,3,4Trihydroxybenzylhydrazide, Acetaminophen-cysteine, methyl-10 hydroperoxy8E,12Z,15Z-octadecatrienoate, Teprenone, Bilirubin, Ouabain, Ramipril glucuronide, Gambogic acid, Cholic acid glucuronide, Sulfoglycolithocholate, 1-Phenyl-2-(diethylamino)-1propanol.

Department of Botany, Deogiri College, Aurangabad. Page 171

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J-13

PHYTOCHEMICAL PROFILE OF CURCUMA INODORA Blatt. RHIZOME EXTRACT

Anand S. Jadhao1 and Anil S. Bhuktar2

1 Department of Botany, B. S. Patel Arts, Commerce and Science College, Pimpalgoan Kale, Ta. Jalgoan (Jamod), Dist. Buldhana, Maharashtra (India). 2 Department of Botany, Vivekanand Arts, Sardar Dalipsingh Commerce and Science College, Samarth Nagar, Aurangabad, Maharashtra - 431 001 (India)

ABSTRACT

Curcuma inodoraBlatt. (Zingiberaceae), rhizome used in the treatment of Psychosomatic disorders, constipation, in muscular pain, also have anti-inflammatory, antipyretic and wound healing activity. Methanolic rhizome extracts screened for detection of phytochemical constituents by using HRLC-MS technique. Metabolites analysis by ESI-Q- TOF-MS revealed presence of 14 major compounds namely 1alpha,25-dihydroxy-26,27- dimethyl-20,21,22,22,23,23-hexadehydro-24a- homovitamin D3, Trp Gln Trp, N-(1-methyl- 2-hydroxy-2-phenyl-ethyl) arachidonyl amine, Arg Lys Ile, Methylprednisolone succinate, (6R)-vitaminD3 6,19-(4-phenyl-1,2,4-triazoline-3,5-dione)adduct, Prednicarbate, N- Palmitoyl-L-serine phosphoric acid, 1-(9Z,12Z,15Z-octadecatrienoyl)-2- (4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycerol,nzonatate(2,5,8,11,14,17,20,23,26- Nona oxaoctacosan-28-ylp-(butylamino)benzoate, (17E)-1alpha,25-dihydroxy- 26,27- dimethyl - 17, 20,22,22,23,23-hexade hydro -24a- homovitamin D3, Salmeterol, 1,2- ditetradecanoyl-sn- glycero-3-phospho-(1'-sn- glycerol) and Digitoxin. This report is the first of its kind to analyze chemical constituents of Curcuma inodora using HR-MS. In addition to this, results of HRMS profile can be used as pharmacognostical tool for identification of Curcuma species. Key words: Curcuma inodora, Phytochemical,HRLC-MS

Department of Botany, Deogiri College, Aurangabad. Page 172

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J-14

Phytochemical analysis and Antibacterial activity of Dolichandrone falcata (Seem), againstselected Human Pathogens. Madhuri M. Deshmukh and Navnath G. Kashid Dept of Botany, Baburaoji Adaskar Mahavidyalaya Kaij, Beed (MS) 431123.

ABSTRACT

The present study aim is identification of maximum phytochemicals and its antibacterial effect against some selected multidrug resistant human pathogens. The selected plant Dolichandrone falcata Seem. Showedpresence of alkaloids, phenolic, Flavonoids, terpenoids, tannins, steroids but absence of mucilage, Tryosine and Tryptophan in all solvent extracts. The plant extracts proved to be very potent against selected human pathogens, which are resistant to multidrugs. The results obtained by antibacterial activity showed equivalent antibacterial zone of inhibition to standard antibiotic Amoxicillin.

Keywords: Dolichandrone falcata, Phytochemistry, Antibacterial activity,

Department of Botany, Deogiri College, Aurangabad. Page 173

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J-15

Evaluation of TLC and GCMS activity of Dolichandrone falcata (Seem). Madhuri M. Deshmukh and Navnath G. Kashid Dept of Botany, Baburaoji Adaskar Mahavidyalaya Kaij, Beed (MS) 431123.

ABSTRACT

In present investigation thin layer chromatography proved presence of different biomolecules. The Methanol extract showed most potent activity among all, so it is tested by using GCMS analysis. Ten major compounds found in methanol extract. In our GCMS analysis observed that 1'-Hydroxy-4,3'-dimethyl-bicyclohexyl-3,3'-dien-2-one(C14H20O2), showed 18.68 % peak area which was highest in all identified ten compounds. The p-Cresol,

2-ehtyl (C9H12O) showed least concentration having peak area 1.45 and retention time was

17.3. The 1'-Hydroxy-4,3'-dimethyl-bicyclohexyl-3,3'-dien-2-one(C14H20O2) might responsible for effective antibacterial activity. Keywords: Dolichandrone falcata, TLC, GCMS analysis.

Department of Botany, Deogiri College, Aurangabad. Page 174

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J-16

Pharmacognostic studies on leaf drug Butea monosperma (Lamk.) Taub. Rupali Biradar 1 and Vikas Gambhire 2 1 Department of Botany, Indraraj Arts, Commerce and Science College, Sillod, Aurangabad 2Department of Botany, Govt. College of Arts and Science, Aurangabad

ABSTRACT

Butea monosperma (Lamk.) Taub. is a tall deciduous tree belonging to family Fabaceae. Its leaves are medicinally exploited to treat several diseases and disorders. Being an important ayurvedic drug it is deliberately adulterated. Adulteration directly effects on quality of drug. Pharmacognostic studies in this leafy drug are carried out to standardize and detect the adulteration in it. Pharmacognostic studies include details of trichomes, stomata, epidermal characteristics and anatomical features of leaves. Physical characters of leaf powder like colour, odour, taste and phytochemical characters like Dry matter (DM), Bulk density, Nitrogen (N), Water soluble nitrogen (WSN), Crude protein (CP), Crude fat (CFat), Crude fibres (CF), Total Ash (TA), Acid insoluble ash (AIA), Acid soluble ash (ASA), Water insoluble ash (WIA), Water soluble ash (WSA), Calcium (Ca), Reducing sugars, Non- reducing sugars, Total sugars, Cellulose, Gross energy (GE), Phosphorus (P) and Extractive values in Water, Acetone, Butanol, Chloroform, Diethyl Ether, Ethyl alcohol, Methanol, Petroleum ether, Propanol, Toluene were also undertaken. The above parameters can be applied in combination to standardize this leaf drug.

Key words-Standardization, Leaf drug, Phytochemical parameters, Adulteratio

Department of Botany, Deogiri College, Aurangabad. Page 175

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J-17

PHYTOCHEMICAL ANALYSIS OF GULWEL TINOSPORACORDIFOLIA (WILD) MIERS. LEAF.

Hirve B.J.

Dept. of Botany Baburaoji Adaskar Mahavidhyalaya Kaij Dist. Beed (M.S.)

ABSTRACT The main objective of current investigation was to study the qualitative preliminary analysis of Tinospora Cordifolia leaf, which is a trial to protect the plant, as leaf regrowth occurs quickly when compared to steam. Gulwel is a very renowned medicinal plant for its versatile pharmaceutical properties. For finding several compounds qualitative phytochemical analysis is very important. Five different solvents viz; water, ethanol, methanol, chloroform and petroleum ether were used to obtain extracts of leaves. These extracts were used for qualitative preliminary phytochemical analysis using standard chemical tests. Data indicates the presence of flavonoids, alkaloids, proteins, phenolic compounds, phytosterols, cardiac glycosides and tannins. Most of the phytochemical components were found in methanolic and Aqueous extracts due to high solubility of active compounds of Tinospora cordifolia leaf in this solvent as compared to other solvents. The presence of these secondary bioactive phytochemicals signifies the importance of these medicinal plants as an efficient source of therapeutic agent. This medical plant is used in traditional medical practices in Beed District. (M.S.)

Keywords: Tinospora cordifolia, phytochemicals, medicinal pant, leaf extract.

Department of Botany, Deogiri College, Aurangabad. Page 176

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J-19

Phytochemical analysis of Bolusanthusspeciosus (Bolus) Harms.

Vidya N.Mhaske1, Namrata A.Gadalkar2, Rohini N.Karjule3, Supriya R.Gawade4 and Sagar S.Bawake5 Post Graduate Department of Botany Ahmednagar College, Ahmednagar

ABSTRACT

Bolusanthusspeciosus is a flowering plant of the family Fabaceae. It is an exotic species grown in Ahmednagar College campus. It is only one individual in Maharashtra grown for its aesthetic value. The plant has medicinal values so the interest was developed in its phytochemical analysis to find out the desired compounds. Different solvents such as water, alcohol, acetone & methanol were used for extraction. All the extracts were subjected to qualitative tests. The phytochemical analyses showed presence of various secondary metabolites in leaves & bark of the plant. The leaf extract showed presence of carbohydrates, starch, protein, flavonoids, alkaloids, reducing sugar and tannin, while tannin was not detected in aqueous extract. The bark extract made in various solvents exhibited presence of flavonoids, alkaloids, tannin and protein.

Keywords:Bolusanthus, Phytochemical, Medicinal, Secondary metabolites.

Department of Botany, Deogiri College, Aurangabad. Page 177

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J-20

Biological mediated silver nanoparticles and investigation of antibacterial, antioxidation potential with qualitative profiling

Ramteke N. M., Salve P.K., Vinchurkar A.S., Kulkarni S.G. Department of Biophysics, Govt.Institute of Science, Aurangabad-431004 MS

ABSTRACT

In this work, we report the synthesis of silver nanoparticles using aloe Vera and vitamin E, piper and nutmeg extract and evaluate its synergistic antibacterial efficacy and antioxidant potential . The synergistic antimicrobial activity of silver nanoparticles with commercially antibiotic against gram negative and gram positive bacteria was evaluated. The antioxidant against potential of synthesized silver nanoparticles was evaluated by DPPH, FERRIC CHLORIDE radical scavenging antioxidant assays and qualitative confirmed by TLC. UV -Visible spectral peak at 440nm and colour change from colourless to brown colour confirmed the formation of AGNPs. Fourier transform infrared spectroscopy (FTIR) revealed the functional group of aloe Vera, piper nutmeg and vitamin E that was responsible for the reduction and capping process of nanoparticles. Piper and nutmeg extract synthesized AGNPs showed good synergistic antimicrobial against gram negative even better than some of the antibiotic used as positive control, they also showed antioxidant potential.

Department of Botany, Deogiri College, Aurangabad. Page 178

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J-21

Ultrasound Induced Germination of Seeds

Shirsat T.T, Jondhale M.B., Kulkarni S.G Department of Biophysics, Govt. Institute of Science, Aurangabad-431004 MS

ABSTRACT

The uses of ultrasound in scientific and medical fields have been revolutionary owing to its beneficial effects. The present study explores the ultrasound exposure induced alterations in the germination of the vegetable seeds. In the present research work ultrasound exposure to vegetable seeds(two sets dry seeds and welt seeds ) were exposed to different frequency(5MHz, 7MHz, 9MHz) & duration(5min,3min) at 8oc.The overall growth characteristics of the exposed seeds as compared to control seeds were monitored to calculate the germination percentage. he germination was stimulated by ultrasound depending on frequency and time of exposure.

Department of Botany, Deogiri College, Aurangabad. Page 179

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J-22

PHYTOCHEMICAL AND PROXIMATE DETERMINATION FROM LEAF OF Cajanus cajan (L.) Millsp. Kolhe P.H, A. Gawate and Patil, R. P*. U.G and P. G. Department of Botany, Deogiri College, Aurangabad 431005 (M.S.)

ABSTRACT

The leaf samples of the plant Cajanus cajan L. were selected for the determination of phytochemical and proximate compounds. The results procured showed the presence of compounds viz., Crude protein, crude fat, crude fibre, carbohydrates, alkaloids, flavonoids, tannins and saponin. In proximate determination the results obtained, showed the presence of crude protein 19.9%, crude fat, 3.10%, crude fibre 8.15%, NFE 61.78%. The phytochemical viz., saponin and other flavonoids have been observed. A.O.A.C (1970) Methods were followed for the determination of proximate constituents. There were variations in the concentrations of, crude protein, crude fat, crude fibre, total ash, acid insoluble ash, nitrogen free extracts.

Keywords: Phytochemical determination, Proximate compounds, Saponin, Flavonoids, crude protein.

Department of Botany, Deogiri College, Aurangabad. Page 180

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J-23

Phytochemical and Anatomical Studies in Amaranthus blitum subsp. oleraceus (L.) Costea

S. N. Sangekar* and V. D. Devarkar**

*Department of Botany, Yogeshwari Mahavidyalay Ambajogai, Dist. Beed **Department of Botany, Shri Chhatrapati Shivaji College, Omerga Dist. Osmanabad

ABSTRACT

Amaranthus blitum subsp. oleraceus (L.) Costea (Amaranthaceae) is a weed commonly occurring in crop fields of Latur region. It is usually collected as wild vegetable with medicinal value. This plant is studied for its macro morphology, anatomy and phytochemistry. Pharmacognostical screening for moisture content, ash analysis for various minerals viz. Potassium, Magnesium, Calcium, Phosphorus; Reducing and non-reducing sugars; Crude proteins & Free amino acids and Alkaloids were also done. Detailed results were discussed in the present paper.

Keywords:Amaranthus blitum subsp. oleraceus (L.) Costea, Phytochemistry, Pharmacognosy, Anatomy

Department of Botany, Deogiri College, Aurangabad. Page 181

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Theme L.

Plant Pathology, Phycology and Cryptogamic Botany

Department of Botany, Deogiri College, Aurangabad. Page 182

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L-1

Effect of Phormidium mucosum Extracts on Growth and Development of Certain Legume Crop Plants

Yadav S.G.

Department of Botany Shivaji Mahavidyalaya Renapur Dist.Latur: 413527 (MS) India

ABSTRACT

Since historical period the algae are used as food, fodder and fertilizer. The algae are known to contain different bioactive compounds. It is observed that several algae have stimulatory effects on seed germination and plant growth. The bioactive compounds present in algae have antimicrobial properties. The biochemicals which are present in algae improve seed germination, seedling development and increases plant tolerance to environmental stresses. Algae are important members of plant world and several of them are significant to man in many ways. They are beneficial in the field of Agriculture, Industry, Medical Science, Space research, Bio-diesel production and Bioremediation. They gain importance in the modern time not only as an alternative potential source of protein for man but also as the primary source of food for aquatic animals. Algae are also considered as rich source of fats, vitamins, minerals and antimicrobial compounds. It was also observed that the algae are found to be stimulatory for the seed germination and plant growth. In the present investigation, extracts of Phormidium mucosum were prepared in different solvants and used to study their effects on seed germination of three legumes viz.Cicer arientinum (Chickpea), Phaseolus radiata (Green-gram) and Vigna mungo (Black-gram). The minimum percentage of germination was recorded in toluene extract (60-65%) which was similar to control and maximum percentage was recorded in acetone extract (95-100%) and followed by hot water, chloroform and petroleum ether (80-85%).The cold water, methanol, and ethanol algal extracts showed good percentage (70-75%).Present investigation reveals that, Phormidium mucosum contains certain growth promoting substances which enhances the seed germination.

KEY WORDS:Phormidium mucosum, seed germination, algal extracts, legumes

Department of Botany, Deogiri College, Aurangabad. Page 183

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L-2

Isolation and Identification of endophytic fungi from chilli (Capsicum annuum L .) plant.

Deshmukh P.D. Department of Botany Late Shankarrao Gutte Gramin Arts, Commerce and Science College, Dharmapuri. Tq- Parli (v.) Dist – Beed.

ABSTRACT The objectives of the research were to study the diversity of leaf , root and stem endophytic fungi of the chilli. Endophytic fungi were isolated from different parts of chilli which are collected from farmers field in local area. These fungal isolates show maximum diversity on PDA and Czapek’s dox agar medium. Total 32 fungal pathogens were found from leaf, stem and root of chilli plant. Among them 10 species were common in all three parts as Aspergillus niger, Aspergillus flavus, Cladoporium sp., Curvularia lunata, Trichoderma polysporum, Penicillium sp., Alternaria alternata, Phoma sp., fusarium moniliformae and Rhizoctonia solani respectively. But some species were specific and found in only leaf as Aureobasidium pullulans, Gliomastix, Gleosporium sp., Stemphyllium sp., etc. Keywords: Endophytic fungi, Chilli (Capsicum annuum, L.) , Isolation , Identification etc.

Department of Botany, Deogiri College, Aurangabad. Page 184

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L-3

Studies on Antagonistic Activities Of Endophytic Fungi Against Soil Borne Pathogens Associated From Leguminous Plants.

Shinde S.Y Department of Botany Late Shankarrao Gutte Gramin Arts, Commerce and Science College, Dharmapuri. Tq- Parli (v.) Dist – Beed.

ABSTRACT

The choice of host plant is of critical importance when working with endophytic fungi. The aim of this study was isolating and charaterizing the antagonistic activities of endophytic fungi. 50 species belonging to 20 genera of endophytic fungi were isolated and identified from the roots of three leguminous plants ( Pigeon pea, broad bean and alfalfa) on PDA and Water agar at 28 ± 20C. Aspergillus (20 sp.), Fusarium (15 sp.) , Penicillium (20sp.) were the dominant genera of which Fusarium oxysporum, fusarium solani, fusarium moniliformae, Penicillium notatum, Penicillium funiculosum , Aspergillus flavus, Aspergillus fumigatus, Aspergillus tubingenesis were the most prevalent. The endophytic fungi of pigeon pea and alfalfa were rich in fungal counts (230 & 210 and 235 & 190 colonies/20 sample on PDA and Water agar respectively.) compared with broad bean (140 & 105 colonies). All isolated fungi to Ascomycota and Deteuromycetes. Keywords: Endophytes, Pigeon pea, Alfalfa, Broad bean, Roots, Fungal isolates etc.

Department of Botany, Deogiri College, Aurangabad. Page 185

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L-6

EFFECT OFANTIBIOTICS ON PROTEASE AND LIPASE PRODUCTION IN SEED -BORNE FUNGI OF SOYBEAN

KESARE U.T. and Ashok Chavan

Department of P.G. studies in Botany, Adarsh senior College Omerga , Dist. Os.bad. Department of Botany,DR.B.M.U.Aurangabad.

ABSTRACT

During the process of biodeterioration, seed mycoflora produce enzymes to degrade protein, carbohydrate and oil. These enzymes called as hydrolytic enzymes. The enzymes which degrade proteins are called protease and enzymes which degrade oil are lipase. It is observed that total thirty species of fungi were isolated from ten varieties of soybean.It is also observed thatAmpicillin completely inhibits the activity of protease production except A. alternata, same results were found with streptomycin except A. glaucus.

KEYWORDS: Antibiotics, Protease,Lipase,Fungi

Department of Botany, Deogiri College, Aurangabad. Page 186

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L-4

ENZYMATIC ACTIVITY OF FUNGI ASSOCIATED ON FRUIT ROT OF COCCINIA INDICA (WIGHT AND ARN).

V. S. Chatage and U. N. Bhale

Department of Botany, Kai. Rasika Mahavidyalaya, Deoni, Tq. Deoni, Dist. Latur,- 413519, (M.S.) India.Research laboratory, Dept. of Botany, Arts, science and Commerce College Naldurg, Tq. Tuljapur Dist.Osmanabad – 413602 (M.S.).,

ABSTRACT

Enzymatic activity e.g. cellulose, amylase, lipase by cup plate and pectinase enzyme by viscomertric method of fungal pathogens causing Ivy gourd fruit rot disease in rot was determined. In- vitro studies; fruit rot pathogens viz., Macrohomina phaseolina and Geotrichum candidus. M. phaseolina showed maximum cellulose activity (26mm). Maximum cellulase activity was showed by G. candidus (30mm). Amylase activity was also maximum enzymatic activity of M. phaseolina (35mm). However, G. candidus (20mm) had a little significant amylase activity. Also recorded significant lipase activity followed by M. phaseolina showed maximum lipase activity (30mm). Amylase activity was showed inG. candidus (18mm)and Pectinase, The extracellular pectinase activity by M. phaseolina (89.94 %) and G. candidus (74.30%) activity after 30mins.

Key words: Coccinia indica, Macrohomina phaseolina and Geotrichum candidus cup plate method, enzymes ect.

Department of Botany, Deogiri College, Aurangabad. Page 187

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L-5

Study of Biddulphiaceae member of Diatoms along the coast of Gholvad and Tarapur, Maharashtra.

Gogari Pankaj K

Department of Botany, N.B. Mehta(V) Science College, Bordi, India.

ABSTRACT

A study of Diatom flora was carried from June 2016 to May 2018 from Gholvad and Tarapur coast of Maharashtra. Samples were collected once in a month before sunrise. Samples were preserved and treated by acid treatment method. Permanent slides were prepared from preserved samples. Photos of diatoms were taken using Nikon research microscope(E100) and Nikon Camera(D7200). Diatoms were identified with the help of standard books and monographs.Out of all species identified Biddulphiaceae members are presented in the current paper.

Key words: Gholvad, Tarapur, Diatoms, Biddulphiaceae.

Department of Botany, Deogiri College, Aurangabad. Page 188

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PRESENT STATUS OF BACTERIAL BLIGHT ON POMEGRANATE FROM OF MAHARASHTRA (INDIA)

B. D. Pandit and N. A. Ghanawat Department of Botany, Yashvantrao Chavan Institute of Science, Satara - 415 001 (M.S.) India

ABSTRACT Pomegranate (Punica granatum L.) is very important in international market as table fruit because of its medicinal uses and nutritive values. Its popular varieties viz. Bhagawa, Ganesh, Mrudula is reported from Pune district. Successful cultivation of pomegranate in recent years has met with bacterial blight caused by Xanthomonas axonopodis pv. punicae is a major disease. A survey has been conducted to check the incidence of disease in Pune District of (M.S.) India. The disease symptoms were recorded on leaves, flowers and fruits of the plant. The disease incidence was recorded from region viz. Shirur, Baramati, Indapur, Purandar and Daund. The disease was found to be severe at Korhale (Baramati), Gulunche (Purandar), Shetphalgadhe (Indapur), Patas (Daund) and Shikrapur (Shirur). The study through light on present status of Bacterial blight on Pomegranate

Keywords: Pomegranate, Bacterial Blight, Xanthomonas axonopodis pv. punicae (Xap).

Department of Botany, Deogiri College, Aurangabad. Page 189

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L-9

Impact of Aeroallergens: on Human Health Hazards.

Kalpana P Ghoshal. Department of Botany, Dhote Bandhu Science College,(M.S.) India.

ABSTRACT

The incidence of allergic diseases is increasing gradually and is a global burden affecting socio economic quality of life. Aeroallergens play a major role in the pathogenesis of respiratory allergic diseases, particularly asthma and rhinitis. Bacteria, fungal spores, pollens, dust mites, insects debris, animal epithelia ( Bioaerosols, and airborne particles) are of perticular importance as triggering factors. Pollens and fungal spores are well studied as allergens amongst all other aeroallergens and are important source of pollinosis.An Aerobiological survey (of ) was carried out exploring through a linked aerobiological work. About 10 pollen grains and various fungal spores were identified and were found responsible causing respiratory allergic diseases like sneezing, hey fever, watering of eyes and nose, rinitis asthematic trouble etc. Aspergillus species were dominant component of the aerospora followed by Penicillium, Curvularia, Cladosporium, Alterneria, Chaetomium, Trichoderma, Mucor and Rhizopus.Alianthus pollen were dominant followed by Riccinus, Xanthium, Amaranthus, Eucalyptus and members of Poaceae.

Key words: Aeroallergens, Pollen, Pollinosis

Department of Botany, Deogiri College, Aurangabad. Page 190

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L-12

SENSITIVITY OF DIFFRENT FUNGICIDES AGAINST DRY ROT OF POTATO

Wakle G.L.

Department of Botany - R.M.I.G. College, Jalna

ABSTRACT

Potato ( Solanum tuberosum L.) is most nutritive food crop. It is native o South America and cultivated all over the world. It get infected by various disease. Among these, dry rot of Potato caused by Fusarium coeruleum (Lib) sacc. is most importatnt disease that cause heavy loss in yield. The sensitivity of different fungicides was tested against Fusarium coreuleum (Lib) sacc. The fungicides i.e. Carbendazim, Calaxin, Captan, Captafol, Difoltan, Dithane M-45, Hexathir, Thiophynate methyl and Thiram Was terted against Fusarium coreuleum (Lib.) sacc. Among these fungicides, Carbandazim Shows maximum and Calaxin and Thiram shows minimum precent control efficacy to Fusarium coreuleum (Lib.) sacc.

Department of Botany, Deogiri College, Aurangabad. Page 191

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L-11

Antimicrobial activity of crude extracts of endophytic fungi isolated from Ventilago denticulata *Rahul K. Dhabale*, Bhagwan M. Waghmare and Suvarna T. Kadam *Department of Botany, Bhagwantrao Arts& Science College Etapalli Dist. * Botany Research Centre, Department of Botany, Maharashtra Mahavidyalaya, Nilanga. Dist. Latur. Maharashtra (India)

ABSTRACT

Endophytic fungi are eukaryotic organisms that live inside plant tissues with symbiotic association. Recently they have been recognized as a valuable source of novel bioactive metabolites for Biotechnological importance as antibiotics, anti-oxidants, anti- cancerous compounds, enzymes, vitamins, texturing agents and pigments. Therefore, different parts of Ventilago denticulata were subjected to the isolation of endophytic fungi such as Trichoderma koningiiand Fusarium oxysporum were screened for antimicrobial activity against Bacillus cereus, Staphyllococcus aureus, Candida albicans, Pseudomonas aeruginosa and Escherichia coli. Also employed for the production of secondary metabolites, which are extracted withethyl acetate and screened for antimicrobial activity by Agar well diffusion method. The results are indicating that, the extract of Trichoderma koningiifound to be effective for maximum inhibition of the Pseudomonas aeruginosa followed by Bacillus cereus, Staphyllococcus aureus and Escherichia coli except Candida albicans. The extract of Fusarium oxysporum proved their maximum inhibition activity against Bacillus cereus followed by Candida albicans, Staphyllococcus aureus and Escherichia coli. except Pseudomonas aeruginosa.

Department of Botany, Deogiri College, Aurangabad. Page 192

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L-13

Study of some diatoms from shivana Takali dam of Kannad District Aurangabad. (Maharashtra)

Anil A. Kshirsagar

UG & PG Department of Botany, Shivaji Arts, commerce and science college kanad.District Aurangabad.

ABSTRACT

The paper deals with study of different types of diatoms present in Shivana Takali dam of Kannad. Shivana Takali dam is an earth fill dam located on ‘River Shivana’ from different sites of dam 11 genera and 16 species of diatoms are observed. During the frequent visits from three different sites Navicula closterium is reported highest in number while Neidium affine repoted lowest in number.

Keywords: Diatom types, water bodies, shivana Takali dam, Kannad.

Department of Botany, Deogiri College, Aurangabad. Page 193

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Department of Botany, Deogiri College, Aurangabad. Page 194

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L-14

Antifungal activity of Vvitex Negudo Linn and Buteamonosparm aginest alternaria hibiscicum Wakle G.L. Department of Botany - R.M.I.G. College, Jalna

ABSTRACT

Hibiscicum abelmoschus Linn. (Kasturi Bhandi) is important medicinal plants, used to control many human and animal diseases and different dis orders. The Seeds and leaves, pods and roots are use ful and have economic important, that are used in manufacture of perfumes, brewing and pharmaceutical industries. The seed are useful for manufacturing perfumes and scants, incent stacks so are very important and has economic value. This economically important plant get affected by various pests and diseases other pathogen are also affecfed like bacteria Viruses and aphides among These, fungi are plays major role for distraction and of seeds.In order to control the leaf spot disease Hibiscicum abelmoschus Linn. Caused by Altarnaria hibiscicum different wild medicinal plant parts were used The phyto exteract of Calutropis procera are useful to reduce the fungal groth of Alternaria hibiscum significately

KEYWARDS : Hibiscus abelmoschus Ambrtee, phyto extract.

Department of Botany, Deogiri College, Aurangabad. Page 195

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L-15

FUNGICIDAL EFFICACY ON FUSARIUM OXYSPORUMCAUSING ROOT WILT OF CHICKPEA

Ingale S.T. and Patale S.S.

Smt.S.K. Gandhi Arts, Amolak Science and P.H Gandhi Commerce College, Kada, Tq. Ashti, Dist. Beed. 414202 (M.S.) India ABSTRACT

Fusarium oxysporum is a soil born fungal pathogen and it is attack on several crops and caused diseases. In the present study tested in vitro potential of two different fungicides i.e., Mancozeb and Carbendazim was evaluated against Fusarium oxysporum. Out of these two fungicides Mancozeb has marked minimum inhibitory effect on Fusarium oxysporum than Carbendazim. Carbendazim is more effective and inhibited the growth of the pathogen.

Key words: Chickpea, Fusarium oxysporum, Fungicides, Inhibition.

Department of Botany, Deogiri College, Aurangabad. Page 196

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L-16

STUDIES ON PHYTOPLANKTON DIVERSITY OF GODAVARI RIVER WATER

Ishwar.B.Ghorade1 and Kirti S. Niralwad2 1Departmen of Environmental Science Kohinoor Arts, Commerce & Science College Khultabad, Aurangabad 2Department of Chemistry Nutan Mahavidhyalay Selu,

ABSTRACT

The term plankton refers to those microscopic aquatic forms having little or, no resistance to the water current and are free floating and suspended in open or pelagic waters. The planktonic plants are referred to as "Phytoplankton (microscopic algae and Bacteria) occurs as unicellular, colonial or filamentous forms. The Phytoplankton sampling and physicochemical parameters on monthly basis were carried out for a period of two years from Godavari River water. Standard keys and other literature were used for identification of different species. Four major groups of phytoplankton (Chlorophyceae Bacilloriophyceae, Cynophyceae and Euglenophyceae.) were studied for diversity and seasonal abundance. Among the groups of phytoplanktons, the population density showed variations due to their adaptability to seasonal changes in water quality. Some plankton population disappeared at a specified period and reappeared during other period. This disappearance may be due to the fact that some species occur in spores, under favorable conditions spore germinate and appear as plankton. Assessment of river water bodies with reference to species diversity of flora was done in three different seasons’ summer, monsoon and winter.

KEYWORDS:Godavari River Water, Water quality, Phytoplankton diversity.

Department of Botany, Deogiri College, Aurangabad. Page 197

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L-17

ADDITIONS OF ZYGNEMATACEAE TO THE MARATHWADA REGION OF MAHARASHTRA

Yadav S.G. and Sabale C.G.

Department of Botany Shivaji Mahavidyalaya Renapur Dist.Latur: 413521 (MS)

ABSTRACT

While working on phycological studies of Bhandarwadi minor irrigation project during January 2016 to December 2017 the author came across some interesting members of zygnemataceae i.e.Mougeotia (08), Zygnema (05) and Spirogyara (08)

Key words:Zygnemataceae ,Marathwada,Maharashtra.

Department of Botany, Deogiri College, Aurangabad. Page 198

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L-18

CYANOBACTERIAL DIVERSITY OF BEVERAGE INDUTRIES WASTE WATER

Talekar S.M. and Baglane A.D.

P.G. Department of Botany, Mrs. K.S.K. College, Beed, Dist-Beed (431122)

ABSTRACT

The Zum Zum cold-drink industry is one of the well known beverages industry located in south part of Beed city. The waste water of cold-drink industry is contaminated due to use of chemicals for manufacturing of products. Beverages Industry waste water shows a variety of algal diversity. Cyanobacteria were dominant in Beverage Industry waste water along with members of Chlorophyceae, Bassilariophyceae and Eugleonophyceae. During the period of June 2017 to May 2018 a total 14 genera and 41 species were recorded Chroococcus 2 ,Gloeothece 1, Aphanocapsa 3 , Aphanothece 3, Synechococcus 1, Rhabdoderma 1, Merismopedia 1, Spirulina 4, Oscillatoria 10, Phormidium 7, Lyngbya 3, Schizothrix 2, Plectonema 1, Scytonema 2.

Department of Botany, Deogiri College, Aurangabad. Page 199

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L-19

Fungal Endophytes Associated with the Indian laburnum (Cassia fistula L.)

Ashfaque M. Khan, Jawed Shaikh and Harshdeep B. Sartape Department of Botany, Maulana Azad College of Arts, Science and Commerce, P.O. Box No-27, Aurangabad (MS)

ABSTRACT

Endophytes are unknown symbionts that live in plant tissues without indicating their presence. The association of endophytes with plants is well established. They not only help in water and mineral uptake but also play an important role in metabolic activities of certain plants. Endophytes play an imperative role to maintain the health of plants, as they can protect or prepare the plant against abiotic and biotic stresses and help in enhancing growth and yields. In present investigation about endophytes in plants, Indian laburnum (Cassia fistula L.), a flowering plant found mostly in India and South Asia belonging to legume family of angiosperm was used. Traditionally the leaves of plant are used for skin diseases, burning sensation, dry cough, dysentery, inflammation, fever etc. Isolates from leaves collected from Aurangabad district of Marathwada region in India showed the presence of fungal endophytes. Fungal strains like Phyllosticta fallopiae, Colletotrichum cobbittiense and Diaporthe brasiliensis was found.

Key words: - Fungal Endophytes, Indian Laburnum, Bioactive compounds.

Department of Botany, Deogiri College, Aurangabad. Page 200

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L-20

Use of Some Plant Extracts as Safe Fungicides against Selected Phytopathogenic Fungi

Gagrepatil V. A. & *Vanmare D. J.

Assistant Professor, Department of Botany, K.K.W.Arts, Sci. and Com College Pimpalgaon Baswant , Maharashtra, India *Professor, Department of Botany, Vivekanand College, Aurangabad, Maharashtra, India

ABSTRACT

The ability of different natural plant extracts was carried out to find out their effects on the fungi mostly some phytopathogenic fungi like Alternaria alternata , Fusarium oxysporum and Aspergillus flavus. In vitro studies were carried out to test the antifungal activity of selected four plant extracts. The results shown that plants extracts had a strong antifungal activity with significant inhibition on the growth of the all tested fungi. Extracts of Azadirachta indica and Jatropha curcas were the most effective to inhibit the growth of the tested fungi. Different concentrations of plant extract of A. indica were studied on the growth of Aspergillus flavus and Alternaria alternata. Findings of this study confirmed that selected plant extracts can be used as natural safe fungicides as biocontrol for phytopathogenic fungi, thus reducing the dependence on the synthetic chemical fungicides. Azadirachta indica extract, which was found to be the most efficient extract, might be a shows potential agent for controlling these fungi.

Keywords: Fungi, Plant extracts, Biocontrol, Fungicide, Phytopathogenic

Department of Botany, Deogiri College, Aurangabad. Page 201

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L-21

ISOLATION AND SCREENING OF SOIL FUNGI ON DIFFERENT CULTURE MEDIA

Patil T.V. and Pangrikar P.P. Department of Botany, R.B. Attal college of Arts science and commerce, Georai, Beed.(MS)

ABSTRACT

In the present investigation an attempt has been made to isolate the soil fungi from different localities. For isolation of fungi soil samples was collected from the different fields in pre sterilised zip lock bags. Soil samples were cultured on the different culture media like Potato dextrose agar, Rose Bengal agar, Czapek dox agar and Glucose nitrate agar by serial dilution method. The culture plates were incubated at 300c temperature and PH was maintained 6.5 for seven days. Fungi were sub cultured to obtain pure culture after getting pure cultures screening of fungi were done on different media for different parameters and fungi were identified by standard protocol.

Keywords: Rose Bengal agar, Czapek dox agar, PH .

Department of Botany, Deogiri College, Aurangabad. Page 202

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L-22

SOME SPECIES OF GENUS SELAGINELLA P. Beauv. FROM MAHARASHTRA

Neel R. S. and Bhuktar A. S.

Department of Botany, Vivekanand Arts, Sardar Dalipsingh Commerce and Science College, Aurangabad

ABSTRACT

Authors are engaged in studies on fern species from Maharashtra since last eight years. They have reported 77 fern species from various localities of Maharashtra (Neel et. al. 2019). After gone through literature it was found that, there is a least work carried out on pteridophytes in Maharashtra. During investigation they came across four species of genus Sellginella P. Beauv. in which two, S. keralensis Dixit and S. repanda (Desv.) Spring are reported first time from Maharashtra. Earlier references available are, Bhuskute (1995) reported S. ciliaris (Retz.) Spring from , Masal and Dongare (2010) have reported S. delicatula (Desv.) Spring from district, Jadhav et. al. (2011) have reported S. delicatula (Desv.) Spring from Koyana wildlife sanctury and Shaikh et. al. (2014) have reported S. delicatula (Desv.) Spring and S. tenera (Hook & Grev.) Spring from region of Maharashtra. In present investigation authors have reported five species (* S. ciliaris on the authority of Bhuskute (1995)) of genus Sellaginella from Maharashtra state. Details will be presented in paper.

Key words- genus, Sellaginella, Maharashtra.

Department of Botany, Deogiri College, Aurangabad. Page 203

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L-23

Fungal Spores of Soybean field, Rajuri (N), Dist. Beed.

J. J. Kshirsagar, G.L. Pachkore, A.N. Dharasurkar

Department of Botany, Vasantdada Patil College, Patoda, Dist.Beed. (M.S)

ASTRACT

Fungal survey was carried out in the Soybean (Glycin max L.) field for a period of Kharip Seasons from 25nd June 2019 to 30th September 2019. For trapping the fungal spores. Tilak air sampler was used. During the investigation the aeromicroflora population includes large number of fungal spores, pollen grains, inscect parts etc. The result showed incidence of varieties of fungal spores in the environment. In this investigation 61 spores were identified during the period of survey. In the Kharip seasons the most dominant spores were Cladosporiun (14.28%) Alternaria (11.37%), Cercospora (1.28%), Curvularia (7.32%), Helminthosporium (7.50%), Periconia (1.20%), Rust spores (5.98%), Nigrospora (4.97 %).

Key word : Soybean field, Tilak air sampler, fungal spores.

Department of Botany, Deogiri College, Aurangabad. Page 204

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L-24

Algal flora of Darna river water, Nasik (M.S)

Yogita S. Patil 1 , H.A. Thakur 2, B.N. Zaware 3, R.Y.K. Science College Nasik

ABSTRACT Fresh water resources like River encompasses are different types of autotrophic phytoplanktons like algae. Algal floras are important biotic components of aquatic habitat. They do determine the tropic status and the quality of water of river and reservoirs. A definite interrelationship exists between various factors such as light, turbidity, nutrient level, planktons in relation to productivity of river. The present research paper, quantitative analysis studies of four groups of algae namely Chlorophyceae, Cyanophyceae, Bacillariophyceae and Euglenophyceae were made for two years at six sampling sites at Darna River; Nashik.

Keyword: Algal flora, quantitative analysis, Darna River

Department of Botany, Deogiri College, Aurangabad. Page 205

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L-25

Invitro Antifungal Efficacy Of Spondias pinnata Kurz. Against Colletotrichum falcatum Went. Causing Red Rot Disease In Sugarcane

Aarti M Patil, Sadat. M. Quazi and Seema. M. Sathe Department of Botany, Maulana Azad College, Aurangabad (MH)

ABSTRACT

The present study was undertaken to evaluate invitro antifungal activity of Spondias pinnata Kurz. against Collettrichum falcatum Went., a pathogen causing red rot disease in sugarcane. The pathogen was isolated from the infected samples of sugarcane stems and identified on the basis of morphological and cultural characteristics using standard literature. The plant Spondias pinnata Kurz. is reported to show very strong antifungal potential against the tested pathogen. The highest mycelial inhibition (88.8%) of the pathogen was achieved by Acetone extract followed by (83%) inhibition by ethylacetate extract and (77.7%) inhibition by ethanol extract. Preliminary phytochemical analysis of the plant extracts showed the presence of secondary metabolites such as alkaloids, carbohydrates, cardiac glycosides, flavonoids, phenols, tannins, steroids and saponins ( only in ethanol extract) while terpenoids and proteins/aminoacids were absent in all the extracts. HPTLC analysis of the extracts was conducted for further separation and standardization of the phytochemicals.

Key words: Red rot, C. falcatum, S. pinnata, antifungal activity, phytochemical analysis.

Department of Botany, Deogiri College, Aurangabad. Page 206

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L-26

Fungal Airspora over Cajanus cajan L.

Nilam Tupe and Suchita Rajurkar

ABSTRACT

Present investigation deals with the fungal airspora over Cajanus cajan L. field. The study was carried out by using Volumetric Tilak Air Sampler in two kharif seasons i.e. 1st July 2017 to 23rd Dec 2017 and 1st July 2018 to 26th Dec 2018. During the study 51 airborne components were collected including insect parts, mycelium, protozoan cyst, pollen grains. Cladosporium and Rust spores were dominant in both the season. Variations were seen among the fungal spores with respect to seasonal changes. The concentrations of fungal spores were compared with meterological parameters.

Keywords: aerobiology, Cajanus cajan L., fungal spore, meteorological

Department of Botany, Deogiri College, Aurangabad. Page 207

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L- 27

Biochemical value changes in infected Banana fruit from Jalgaon district (MS).

Sadhana Salve and Suchita Rajurkar

ABSTRACT

Banana is an important fruit crop in India. Banana fruit plays a vital role in human nutrition by providing the essential growth factors such as vitamins and minerals in human daily diet. During ripening, starch contents are gradually converted to sucrose, glucose and fructose. Semi ripened and ripened fruits are susceptible to attack by microorganisms because of their high moisture and rich nutrients. The banana fruit was infected by Verticillium theobromae, Fusarium moniliforme, Cladosporium musae and Fusarium oxysporum. These fungal pathogens reduced biochemical quantity of banana fruit such as dry weight, crude fiber, ash, crude protein, reducing sugar, calcium, potassium, phosphorous and nitrogen.

Keywords: Banana fruit, Biochemical, fungal pathogens.

Department of Botany, Deogiri College, Aurangabad. Page 208

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L-28

Isolation and identification of endophytic fungi from Solanum nigrum

R. B. Allapure

Associate Professor and Head

Dept. of Botany, Maharashtra Udayagiri Mahavidyalaya, Udgir.

ABSTRACT Medicinal plants are a rich source of natural products used to treat many diseases; therefore, they are the basis for a new drug discovery. Plants are capable of generating different bioactive secondary metabolites, but a large amount of botanical material is often necessary to obtain small amounts of the target substance.Endophytes are able to produce highly diverse compounds, including those from host plants that have important biological activities. In the present work isolation of endophytic fungi was carried out.Twenty five isolates of endophytic fungi were collected from the leaves of Solanum nigrum Linn. All isolates wereidentified based on colony morphology and examination of spores and fruiting bodies using stereo and light microscopes. Twenty isolates belonging to 05 genera were recorded, viz. Cladosporium sp., Trichoderma sp., Monilia sp., Fusarium sp., Penicillium sp,while 07 strains were unidentified. The dominant genera found were Monilia sp. and Fusarium sp. Thus, there seemed to be a significantdifference in the genera of endophytic fungi from Solanum nigrum Linn.

Keywords: Solanum nigrum Linn , endophytic fungi.

Department of Botany, Deogiri College, Aurangabad. Page 209

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L-29

Assessment of Morphological Characteristics, Growth and Sporulation of the field isolate Magnaporthe oryzae ch1.

Kalpana Kulkarni and Swati Peshwe

ABSTRACT

Rice is the most important staple food crop after wheat. India is one of the largest producer of rice accounting yearly 20% of the world’s population. Magnaporthe oryzae is the most devastating pathogen causes blast disease in rice. It is among ten top most important fungal and bacterial diseases in plants and are found in almost every paddy field where rice is grown. It has been reported in recent years about its occurence as a wheat blast as well. Magnaporthe oryzae is an infectious fungus and is distributed worldwide across more than 85 countries. This fungus attacks rice host at any growth stage of rice plant parts or infect rice grain after harvest causing considerable losses in the quantity and quality of the produce. Various strategies are being deployed to control disease on field.

Blast fungus is highly evolving and hence characterization of the fungus in laboratory is very crucial aspect in the study of blast disease in depth. In the present study, different carbon, nitrogen and mineral sources were used in combination as media components so as to understand nutritional requirements of this phytopathogen. Natural and synthetic media formulations were assessed for mycelial yield using broth media. Radial growth pattern was th observed on 11 day of inoculation on solid media. Dextrose, yeast extract and K2HPO4 were found to play a key role in the growth of this fungus in laboratory. In natural media, Oat meal agar served to better media for growth and sporulation as compared to potato dextrose agar and Soyabean Casein agar. Triseptate spores were observed under haemocytometer. Total viable count was performed for sporulation on media plates. Morphology of fungal mycelia on various media plates showed varied patterns of septation and melanization which reveals role of pigmentation in the sporulation.

Keywords:Blast disease, phytopathogen, rice, mycelial yield, melanization.

Department of Botany, Deogiri College, Aurangabad. Page 210

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L-30

Diversity of Wood rotting Aphyllophorales from Bhoom tahsil (Osmanabad district) Maharashtra

F. H. Chouse1, V. P. Mali2

1 Milliya Junior College of Arts and Science, Beed – 43112 (MS) India, 2 J. Watumull Sadhubella Girls College, – 01 (MS) India,

ABSTRACT

Bhoom tehsil comes under the territory of Osmanabad district which is located between 18°27'32.2812" North Latitude and 75°39'41.6700" East Longitudes. The survey area was located on and was found mostly rocky in formation having cultivated forest areas. Being barren, the land was dried except for the rainy season. The damage of the trees was mostly due to the wood spoilage caused due to wood rotting macro fungi. After monsoon, these organisms revive and begin to produce spores very quickly. Even though these fungi have been considered as destroyer of dicotyledonous wood, being the member ecosystem they may have positive role in recycling process of decomposed wood into humus.The present research survey was carried out during July 2014 till December 2017. Around more than 36 specimens were collected found on different host of angiospermic trees. All the wood deteriorating lignicolous macro fungi belonging to order Aphyllophorales fall under the 14 taxa. Species enumeration shows that Phellinus were abundant followed by Ganoderma and Trametes, Scytinostroma. Frequently occurring hosts were Acacia arabica, Leucaena leucocephala, Gliricidia sepium, Mangifera indica, Tamarindus indica,Azadirachta indica, etc. Phellinus badius and Ganoderma lucidum was also observed as dominating representative of these areas. Species like Duportella tristicula, Irpex vellereus,Loweporus tephropours, Navisporus floccosus, Podoscypha petalodes, Porostereum spadiceum, Pyrofomes albomarginatus and Trametes leolina, Trametes variegate, Pellinus allardii are rarely found due to unfavourable condition for the development.

Key Words : Wood rotting fungi, Bhoom, Aphyllophorales

Department of Botany, Deogiri College, Aurangabad. Page 211

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L-31

Mycoflora Isolation Form White Pumpkin (Cucurbita pepo L.)

Anil U. Kulkarni & Shrimant A. Survase

Department of Botany, Lal Bahadur Shastri Senior College, Partur Dist. Jalna 431501, (M.S.) India

ABSTRACT

The word “Pumpkin” is derived from the Latin word pepo, which is a botanical term for fruit with a hard rind and fleshy interior. Commonly, any Cucurbita fruit with an orange color is considered a Pumpkin; however, Cucurbita cultivars with white fruit, such as ‘Lumina’ and ‘Cotton Candy’ are also considered pumpkins. In the United States, any cultivar of C. pepo L.used for baking, food stock, or Halloween jack-o-lanterns is considered a pumpkin. The disease is caused by a fungal pathogen that infects many different vegetable hosts, survives in our soils indefinitely, and spreads quickly within and among fields in seasons with typical midwestern temperatures and rainfall patterns. Symptoms on Pumpkins often are discovered first on the surface of mature fruit, but vines of infected plants eventually collapse and die. The soft rot associated with a rapidly expanding area of white, cottony mold on any part of the fruit is a very characteristic symptom of the disease. Present investigation had been made on the isolation of fungal biodiversity from the white pumpkin .The sample were put on PDA media, isolation and identification were made with help of standard literature. Ten dominating fungi were isolated from various sample of pumpkin. Fungi like Aspergillus niger, A. flavus; Fusarium, Phytopthora and Penicillium were dominated. Post harvesting, fruits shows maximum fungal biodiversity.

Key Word: Pumpkin, PDA, Fungal Biodiversity.

Department of Botany, Deogiri College, Aurangabad. Page 212

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L-32

In Vitro Dominance of Bacillus amyloliquefaciensRRR15for Microbiological Control of Sclerotium rolfsii Sacc., A Stem Rot Pathogen of Groundnut

R. R. RAKH * L. S. RAUT **and S. M. DALVI# * Department of Microbiology, Shri Guru Buddhiswami Mahavidyalaya Purna (Jn.) – 431511 ** Department of Microbiology, Sant Tukaram College of Arts and Science, Parbhani – 431401 # Department of Botany, Shri Guru Buddhiswami Mahavidyalaya, Purna (Jn.) – 431511

ABSTRACT Stem rot disease caused by Sclerotium rolfsii Sacc. is one of the most important disease of groundnut causing major crop losses. To pursuit for the effective Bacillus spp. as microbiological control of Sclerotium rolfsii Sacc., 189 Bacillus spp. were isolated from different rhizospheric niches of healthy plants, and primarily screened in vitro for the antagonistic activity against Sclerotium rolfsii, by dual culture technique. During the primary screening, Bacillus spp. RRR15,found highly effective in killing the phytopathogen, Sclerotium rolfsii in Vitro. The Bacillus spp. RRR15, effectively slaughter the growth of phytopathogen, Sclerotium rolfsii whose percent inhibition recorded as 87.5. The Bacillus spp. RRR15 later identified as Bacillus amyloliquefaciens RRR15 by 16 S rRNA sequencing.

Key words: Groundnut, Stem rot, Sclerotium rolfsii, Bacillus amyloliquefaciens RRR15.

Department of Botany, Deogiri College, Aurangabad. Page 213

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L-33

Investigation of Phosphate Solubilizing Bacteria (PSB) from Rhizospheric Niches of Healthy Plants

SANJAY M. DALVI*, V. N. KADAM and R. R. RAKH#

* Department of Botany, Shri Guru Buddhiswami Mahavidyalaya, Purna (Jn.) – 431511 # Department of Microbiology, Shri Guru Buddhiswami Mahavidyalaya, Purna (Jn.) – 431511

ABSTRACT

Rhizospheric niches of healthy plants play an important role in the maintenance of health and growth of plant either by providing valuable nutrients or protecting plant from soil borne pathogens. The aim of present study was to use the rhizospheric niches of healthy plants to isolate efficient phosphate solubilizing bacteria. The soil samples from rhizospheric niches of Neem, Soya bean, Bavanchya and Tur were collected from different fields in the sterile polythene bags and brought to the laboratory. All the rhizospheric soil samples were tested for phosphate solubilizing bacteria on Pikovskaya’s agar by serial dilution method. Among the rhizospheric soil samples screened, rhizospheric niches from the Soya bean shown highest phosphate solubilizing bacteria, 114 than the other rhizospheric soil sample. The rhizospheric niches of Tur, Neem and Bavanchya have shown 47, 07, and 02 phosphate solubilizing bacteria. Over all 170 phosphate solubilizing bacterial isolates were isolated from different niches. Out of which 4 isolates, RRR18, SMD36, SMD38 and SMD40 were found to produce more than 5 mm zone of tri-calcium phosphate solubilization on Pikovskaya’s agar plates after 9 days of incubation.Out of these 4 bacterial isolates, two bacterial isolates, namely SMD 38 and SMD 40when quantitatively tested, showed maximum P solubilization on 7th day in PKV broth supplemented with tri – calcium phosphate as 444 μg/ml and 421 μg/ml respectively.

Key Word: Rhizospheric niches, Pikovskaya’s Agar, Phosphate Solubilizing bacteria.

Department of Botany, Deogiri College, Aurangabad. Page 214

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L-34

ALGAL FLORA OF MAIZE FIELD SOIL IN OF MAHARASHTRA.

Milind J. Jadhav and Swati G. Wagh*

Department of Botany, Sir Sayyed College, Roshan Gate area, Aurangabad-431001 (M.S.) India.

*Department of Botany, Shri. Baneshwar Arts, Commerce and Science College, Burhannagar, Tal.And Dist.- Ahmednagar-414002 (M.S.) India.

ABSTRACT

Soil algae are one of the significant components of soil microflora. They play an important role in fertility of soil. Blue green algae fixes atmospheric nitrogen and increases the fertility of soil. The cultivated field ecosystem provides a favourable environment for the growth and development of algae. Present research work deals with the study of algal flora of Maiza (Zea mays L.) field, located in Shrirampur tehsil area of Ahmednagar district of Maharashtra. In order to study algal flora of maize field, algal samples from moist places of field were collected at regular intervals from July 2017 to October 2017. Bold’s basal medium was also used to culture algae from soil of maize field. Collected and cultured algal samples were observed with the help of standard literature on algae. A total of 47 species under 29 genera belonged to Chlorophyceae, Bacillariophyceae and Cyanophyceae were recorded. Cyanophycean algal taxa were found dominant in the soil of maize field. Gloecocystis gigas, Gloeocystis major, Chlorococcum humicola, Chlorella vulgaris, Nitzschia palea, Aphanothce nidulans, Aphanothece saxicola, Oscillatoria obscura, Phormidium jenkelianum, Phormidium molle, Phormidium usterii, Microcoleus acutissimus, Microcoleus lacustris and Plectonema gracillimum were found abundant. Algal flora of maiz field is rich and it is in diverse form.

Key words: Algal flora, maize field, soil.

Department of Botany, Deogiri College, Aurangabad. Page 215

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L-35

Influence of post-harvest fungi on biochemical changes of selected edible nut seeds stored in commercial markets in Marathwada Suvarna T. Kadam, Bhagwan M. Waghmare and Rahul K. Dhabale Botany Research Centre, Department of Botany, Maharashtra Mahavidyalaya, Nilanga (MS)-India

ABSTRACT

The role of fungi which were associated with edible nuts and their influence on change in biochemical studies have been carried on some widely consumed nuts namely, almond, cashew nut and pistachio nut which were collected from different market places of Marathwada region of Maharashtra. Fifteen samples of each nut were utilized for isolation of fungi by using. It was possible to isolate, the fifteen fungal species belonging to six genera. Percent frequency of fungal incidence was varied in almond, cashew nut and pistachio nut from which highest count of Aspergillus niger was observed in pistachio nut followed by cashew nut (100% and 73% respectively) and Rhizopus species was found about 80% in almond.Ten species of isolated fungi were utilized in order to know their role in biochemical change in proteins, fats and carbohydrates in almond, cashew nut and pistachio nut. The results were remarkable to note that the highest degradation of protein, fat and carbohydrate were found in cashew nut as compared with almond and pistachio nut.

Keywords:- Aspergillus niger, nuts, post-harvest, Rhizopus species.

Department of Botany, Deogiri College, Aurangabad. Page 216

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L-36 Prevalence of Bacterial Pathogens in Vegetables Commonly Used as Salads

Sayed Rizwan A and Bagmar Chitra Department of Microbiology Sir Sayyed College of Arts, Commerce and Science Aurangabad, Maharashtra, India - 431401

ABSTRACT

Microbiological control is very important in food and hotel industries to prevent food borne diseases. The present research work, therefore, was undertaken to assess the total viable count and bacteriological quality of salad vegetables collected from several places of Aurangabad, Maharashtra, India. A total of 200 vegetable salad samples were collected and examined for enteric pathogens grown on EMB (Eosin Methylene Blue) S-S (SalmonellaShigella) and MacConkey’s Agars and found that 60 vegetables (30.0%) were culture positive. The food pathogens were identified by the conventional methods. Incidence of Shigella was found maximum (10.5%) followed by Salmonella sp. (10.0%) whereas the E. coli noted slightly less incidence that is 9.l5 %. Among all the collected salad vegetables, carrot was the highest contaminated. Key words : Food borne diseases, vegetables, Shigella, Salmonella, E. coli.

Department of Botany, Deogiri College, Aurangabad. Page 217

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L-37 CHLOROPHYCEAN ALGAL FLORA OF LONAR CRATER LAKE

RAFIULLAH M. KHAN1 AND MILIND J. JADHAV2

1Department of Botany, Kohinoor College, , Dist. Aurangabad -431101(M.S.) India.

2Department of Botany, Sir Sayyed College, Roshan Gate, Aurangabad -431001(M.S.) India.

ABSTRACT

Lonar lake is a natural water body. It is situated in of Maharashtra state. is believed to be originated due to meteoritic impact. The Lonar lake water appears to be saline due to high concentration of dissolved solids and total suspended solids.In present study algal samples from selected sites of Lonar lake were collected at monthly intervals, October 2015 to September 2017. Acid washed collection bottles were used for the collection for algal samples. In present study 11species under 10 genera have been identified and recorded. Algal genera such as Gloeocystis gigas, Gloeocystis major, Chlorella valgaris and Coelastrum microporum were found dominant. Unicellular and colonial taxa of green algae were recorded.

Key words: Chlorophycean algae, salinity and alkalinity, Lonar lake.

Department of Botany, Deogiri College, Aurangabad. Page 218

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L-38 Natural Saponin in Sapindus Emarginatus Controls the Protein Loss In Phaseolus Aconitifolius Grains Caused by Callosobruchus Chinensis

Bajad P. N., *Pardeshi A. B. and Pagore V. P.

ABSTRACT Saponin containing Pericarp of Sapindus emarginatus fruit were tested for their effect on Callosobruchus chinensis. It is most widespread and dreadful pest. The whole grain of Phaeseolus aconitifolius were found to contain 23.3 % protein, while the infected hollow grains had reduced protein contents. On exposure to ethanol, methanol and aqueous extracts of Sapindus emarginatus, the percent protein loss in total grains at the said concentrations was highly reduced indicating the saving of the proteins against the damage by Callosobruchus chinensis. Protein loss was also minimized in different concentrations of ethanol, methanol and aqueous extracts.

Keywords: saponin, Callosobruchus chinensis, Phaeseolus aconitifolius

Department of Botany, Deogiri College, Aurangabad. Page 219

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L-39 Qualitative study of Zooplankton in relation to anthropogenecity at Nathsagar Wetland of Paithan Taluka of Aurangabad District

Rahul Mahamuni and Kshama Khobragade* *Head, Department of Environmental Science, S.B.E.S. College of Science, Aurangabad – 431 001 (M.S.) India.

ABSTRACT:

The present Nathsagar wetland is situated 40 kms south of Aurangabad at Paithan. This town is situated on the right bank of river Godavari and is at latitude 19o39’19” north and longitude 75o26’2” east. . Nearby downstream area of area lot of anthropogenic activities takes place especially near Eknath Maharaj Temple throughout the year ritual ceremonies lead to extraction of water table by siltation. Also the agricultural activities add up to the level of pressure to the clean biota thereby causing disturbance in the life cycle of various aquatic creatures, phytoplankton and zooplankton. Wetland is partially polluted by domestic sewage besides washing and bathing purposes. Qualitative study of zooplankton in the Nathsagar wetland of Paithan Taluka of Aurangabad District of Maharashtra state was studied during January 2019 to December 2019. Among the Zooplanktonicgroups, Rotifers found frequently followed by Copepoda, Ostracoda and Cladocera during the year 2019.

Key words: Zooplankton, Frequency, Wetland and Anthropogenecity.

Department of Botany, Deogiri College, Aurangabad. Page 220

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L-40 Inhibitory Activity of Syzygium cumini L. Leaf Extracts on Fungi 1Satpute S.B. and Vanmare D.J. 1Dept. of Biology, ShivChhatrapati College, N- 3, Cidco, Aurangabad, Maharashtra, India Department of Botany Vivekanand Arts, Sardar Dalipsingh Commerce and Science College, Samarthnagar, Aurangabad (M.S.) 431001.

ABSTRACT From Vedic era to save the crops from the attack of pests and diseases, peoples used crude chemical, animal and plant materials. Biological control is cost effective, eco-friendly and an innovative. Synthetic chemicals cause severe and long-term environmental pollution and are highly and acutely toxic and can even cause cancer in humans and wild animals. Also, pathogens may become resistant to many of these chemicals. The aim of the present work was to determine inhibitory activity of Syzygium cumini L. leaf extracts on fungi. The efficacy was studiedby poisoned food technique against pathogenic fungi. The leaf extract was used at various concentrations viz., 10-100%, while the five pathogenic fungi under investigation were Fusarium incarnatum, Alternaria citri, Colletotrichum musae, Colletotrichum sp. and Gibberella avenaceum. Among the fungi maximum growth inhibition was observed in case of Gibberella avenaceum (66.54 %), followed by Fusarium incarnatum (65.04 %), Alternaria citri (55.64 %), Colletotrichum musae (26.02%) and Colletotrichum sp. (8.96 %) at 100% concentration. The differences in percent inhibition among the fungi as well as due to various concentrations of leaf extract were statistically significant (p=0.01). Keywords:Syzygium cumini L., Alternaria citri, Gibberella avenaceum, Fusarium incarnatum, Colletotrichum musae.

Department of Botany, Deogiri College, Aurangabad. Page 221

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L-41

EFFECT OF DIFFERENT ENVIRONMENTAL CONDITIONS ON THE ANTIMICROBIAL ACTIVITY OF PSEUDOMONAS FLUORESCENS AGAINST ALTERNARIA ALTERNATA(FR.) KEISSLER CAUSING FRUIT ROT OF POMEGRANATE

Bharade Sunita S.

Department of Botany, Badrinarayan Barwale Mahavidyalaya, Jalna. Affiliation: Dr. Babasaheb Ambedkar Marathwada University, Aurangabad. (M. S.) Email- sunita..bharade [email protected]

ABSTRACT

Fruit rot disease of Pomegranate (Punica granatum L). is caused by Alternaria alternata. During present investigation Aa-21 resistant isolate of this pathogen was used. Present investigation was undertaken to find out the antimicrobial potential of Pseudomonas fluorescensin vitro. Four environmental conditions were testes with different concentration and ranges , exhibited significant mycelial growth inhibition of A. alternata. Physical factors such as temperature and PH influence the activity of biocontrol agent , At 250C Percent inhibition was (84.77 %) , at PH 5.0 ( 84.77%) , at 30gm carbon source percent inhibition was ( 82.85%), and at 3gm nitrogen source it was (80.18%). Decrease in concentration of carbon and nitrogen source there is decrease in growth of resistant isolate, carbon and nitrogen have been established as essential elements in the infection process of plant pathogens.

Keywords : Alternaria alternata, Fruit rot, Pseudomonas fluoresence, Pomegranate, Resistant isolate, Percent inhibition, environmental conditions.

Department of Botany, Deogiri College, Aurangabad. Page 222

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L-42

Study of Coscinodiscus Phytoplankton diversity of Tarapur and Gholvad coast of Maharashtra

Pankaj K. Gogari

HOD Department of Botany, N.B. Mehta Science college, Bordi, Maharashtra -401701

ABSTRACT

Diatoms were collected for a period of 2 years from June, 2016 to May 2018. Tarapur is 115 km North of Mumbai at Latitude: 19.850N Longitude: 72.700E latitude. Tarapur atomic Power Station is 2 km from this site. Gholvad is situated 140 km North of Mumbai at 20°5'31"N Longitude 72°43'57"E Latitude. In all 14 species of Coscinodiscus are reported from both coasts.

Keywords: Diatom, Phytoplankton, Tarapur, Gholvad, Centrales, Pinnales.

Department of Botany, Deogiri College, Aurangabad. Page 223

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L-43

Efficacy of fungicides against Stemphylim blight and Purple blotch of Garlic (Allium sativum L.)

A.R Kolte and S.S Patale

S.K. Gandhi Arts, Amolok Science and P.H Gandhi Commerce College Kada. Dist- Beed. ABSTRACT

Garlic (Allium sativum L.) is an herbaceous plant belong to family Alliaceae grown for its edible bulbs and leaves. It is a most important vegetable, spice and medicinal crop produced on large scale throughout the world. Garlic is a good source of vitamins, carbohydrates, fibers, protein, moisture and minerals. It is one of the important commercial vegetable crops cultivated in India. The crop is attacked by various fungal diseases which result in deteriorating the quality and also yield losses. In the present study, in vitro study was conducted for evaluating efficacy of some fungicidesin different concentrationsfor the management of Stemphylium blight and Purple blotch of garlic. Carbendazim showed maximum inhibition against Stemphylium vesicarium (96.06%)and Alternaria porri (92.9%) inhibition followed by Mancozeb (85.86% inhibition ), Benomyl (83.96 % inhibition) against Stemphylium vesicarium and Benomyl (80.9% inhibition) and Mancozeb (73.36% inhibition) anginst Alternaria porri. Copper oxychloride less effective against both fungal pathogens Stemphylium vesicarium (79.86% inhibition)andAlternaria porri (72.46% inhibition).

Keywords: Garlic, Pathogenic fungi, fungicide.

Department of Botany, Deogiri College, Aurangabad. Page 224

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L-44

Phytoplankton Seasonal Abundance in Relation to Physico-Chemical Features in Mahapoli Lake, Bhiwandi, Maharashtra

Rafatunisa nahri Sir Sayyed College,Aurangabad (m.s)India.

ABSTRACT Physico-chemical analysis and Phytoplankton survey of the Mahapoli Lake was carried out on monthly basis for the period of one year from February-2016 to January-2017. PH determined alkaline nature of the lake ranging 7.2 to 7.9, Dissolved oxygen 4.2-7.77mg/l was recorded. Alkalinity (97 to 183mg/l), total Hardness (69 to 142 mg/l), chlorides (45 to 63 mg/l) and the nutrients like phosphates and nitrates were at maximum in summer and minimum in rainy season. During the study period, out of 19 algal genera, Cyanophyceae with 6 genera, Chlorophyceae with 9 genera and Bacillariophyceae with 4 genera were recorded. The highest numbers of Phytoplankton were recorded in post monsoon and lowest in monsoon.

Keywords: physico-chemical, Phytoplankton, Cyanophyceae, Chlorophyceae-

Department of Botany, Deogiri College, Aurangabad. Page 225

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L-45

Biosynthesis and characterizations of silver nanoparticles from Aspergillus

Taur S. A. and Vanmare D.J. Dept. of Botany, Vivekanend college, Aurangabad ABSTRACT

Development of eco-friendly procedures for the synthesis of metal Nanoparticles through biological agents is evolving. In the present study five fungi were isolated from soil, seed and different deteriorated plant parts. The isolated are Aspergillus flavus, A.terreus, A.oryzae, A.nidulans, and A.nigar. These fungi were induced for the extracellular biosynthesis of silver nanoparticles (Agno3).The characterizations of silver nanoparticles was carried out by visual and photometric methods.

Keywords – Silver-nanoparticles, UV spectroscopy, Aspergillus

Department of Botany, Deogiri College, Aurangabad. Page 226

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L-46

Effect of Phormidium mucosum Extracts on Growth and Development of Certain Legume Crop Plants

Yadav S.G.

Department of Botany Shivaji Mahavidyalaya Renapur Dist.Latur: 413527 (MS) India

ABSTRACT

Since historical period the algae are used as food, fodder and fertilizer. The algae are known to contain different bioactive compounds. It is observed that several algae have stimulatory effects on seed germination and plant growth. The bioactive compounds present in algae have antimicrobial properties. The biochemicals which are present in algae improve seed germination, seedling development and increases plant tolerance to environmental stresses. Algae are important members of plant world and several of them are significant to man in many ways. They are beneficial in the field of Agriculture, Industry, Medical Science, Space research, Bio-diesel production and Bioremediation. They gain importance in the modern time not only as an alternative potential source of protein for man but also as the primary source of food for aquatic animals. Algae are also considered as rich source of fats, vitamins, minerals and antimicrobial compounds. It was also observed that the algae are found to be stimulatory for the seed germination and plant growth. In the present investigation, extracts of Phormidium mucosum were prepared in different solvants and used to study their effects on seed germination of three legumes viz.Cicer arientinum (Chickpea), Phaseolus radiata (Green-gram) and Vigna mungo (Black-gram). The minimum percentage of germination was recorded in toluene extract (60-65%) which was similar to control and maximum percentage was recorded in acetone extract (95-100%) and followed by hot water, chloroform and petroleum ether (80-85%).The cold water, methanol, and ethanol algal extracts showed good percentage (70-75%).Present investigation reveals that, Phormidium mucosum contains certain growth promoting substances which enhances the seed germination.

KEY WORDS:Phormidium mucosum, seed germination, algal extracts, legumes

Department of Botany, Deogiri College, Aurangabad. Page 227

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L-47

Studies on fungal foliar diseases of Adulsa (Adhatoda zeylanica Medic) B.M.Kareppa, Head of Botany & Biotechnology Dept., Dnyanopasak (DSM) College, Parbhani-431401 (MS), India.

ABSTRACT

Adulsa (Adhatoda zeylanica Medic). is a sub-herbaceous and evergreen medicinal plant viz. indigenous to India. It is commonly known as Adhatoda, Adulsa, Vasaka, etc. This plant grown as fencing and as an ornamental plant in pots. The utilization of this plant has been known since ancient time and is being used in different formulations of Ayurveda. Since 200 yrs, roots and leaves of this plant are being used to cure cough, rheumatism, malaria, fever, chronic fever, intrinsic hemorrhage, asthma, leprosy, skin diseases, piles and many respiratory disorders. It is also reported as an expectorant, abortificiant, antimicrobial, antitussive and anticancerous. Hence, this plant has great demand in market in the form of crude drugs. In India, the total demand is above 500 t/yr viz. congregated from wild populations. The leaves, which have great medicinal importance are infected due to attack of fungal pathogens i.e. Alternaria alternata causing leaf blight; Aecidium adhatodae and Chnoospora butleri causing leaf rust; Colletotrichum dematium causing anthracnose, while Cercospora adhatodae, Colletotrichum capsici, Drechslera speciferum, Phoma vasicae and Corynespora cassicola causing leaf spot. Therefore, these fungal pathogens are responsible for decrease in active ingredients of plant, hence selected for detailed studies. The present investigation deals with Survey of fungal diseases.

Department of Botany, Deogiri College, Aurangabad. Page 228

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L-48

HITHERTO ON UNREPORTED MACROFUNGI FROM AURANGABAD (M.S.) INDIA Vishal Wakode and Sangeeta Ahuja Department of Botany, Sir Sayyed College, Aurangabad

ABSTRACT: Present study deals with the exploration and enumeration of diversity of macrofungal species were recorded from Aurangabad, Maharashtra. This region extends over an area of 139 km2 lies and still unknown due to unawareness and less attention towards this subject.The floristic composition of this region has been studied earlier by several workers, but the fungus which forms an important part of the ecosystem has been largely neglected in a biodiversity studies. The investigation was first time carried out in this region to explore macrofungi which have socio-economic and medicinal importance. The survey was conducted from June 2016 to December 2018 in six different locations which having dry, deciduous, scrub type of forest vegetation with barren rocky patches scatter all over the region. A total number of 44 macrofungal specimens in 23 genera belonging to 17 families were first time recorded in this region. Dominating species belong to genera Termitomyces followed byVolvariella, Agaricus, Coprinellus, Leucocoprinus etc.The detailed report of the study has been presented here.

Keywords: Macrofungi, Diversity, Habitats.

Department of Botany, Deogiri College, Aurangabad. Page 229

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L-49

STUDY THE EFFECT OF SUBSTRATE AND NON SUBSTRATE MEDIUM ON THE PECTINASE ACTIVITY OF FUNGI FROM VEGETABLES WASTE

*Balwan W. Kamble, *Shrimant A. Survase, *Anil U. Kulkarni and **Ashok M. Chavan *Department of Botany, Lalbahadur Shastri Sr. College, Partur Dist. Jalna. 431501. **Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad – 431004 (M.S.) India

ABSTRACT

Many plants cell wall contain pectin and it degrades by the microorganisms for their growth. Pectinase have been used in several conventional industrial processes over the years, such as textile, plant fiber processing and tea. In order to study the production of pectinase by ten dominating fungal species associated with vegetable waste were tested on substrate (pectin nitrate) and non substrate (glucose nitrate) medium. Pectinase activity was determined by viscometrical method. The result revealed productions of pectinase were tested on substrate and non substrate broth medium. The fungi were subjected to different time intervals and pectinase enzymatic activity representing percent loss of viscosity. The highest pectinase activity was obtained by Penicillium notatum, Trichoderma viride and Aspergillus niger as compare to other fungi like Aspergillus flavus, A. fumigatus, Alternaria alternata, Curvularia lunata, Fusarium oxysporum, Penicillium spp. and Rhizopus stolanifer at different interval time 20, 40, 60 min.

Key word: Substrate, non substrate medium, Ostwald viscometer, fungi, pectinase activity, vegetable waste.

Department of Botany, Deogiri College, Aurangabad. Page 230

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L-50

Isolation and screening of some Aphyllophorales for production of Laccase

Rangnath G. Kanthale and Aparna S. Taware

Dept. of Botany, Deogiri College, Aurangabad.

ABSTRACT-

Some Aphyllophorales were collected from different regions and identified by using standard keys. Malt extract agar plates were used for pure culture of these Aphyllophorales. Pure cultures were screened for production of laccase with different substrates like ABTS, Tannic acid and Napthol. Ganoderma and Phellinus were tested positive for production of laccase.

Keywords- Aphyllophorales, Laccase, ABTS.

Department of Botany, Deogiri College, Aurangabad. Page 231

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L-51

FUNGAL ENDOPHYTES : POTENTIAL SOURCE OF INDOLE ACETIC ACID Narwade P. S. and Taware A. S. Dept. of Botany, Deogiri college Aurangabad

ABSTRACT: In the present study endophytic fungal strains isolated from Cyanodon dactylon were examined for their potential to secrete phytohormone such as indole acetic acid (IAA). Out of 15 isolates 10 isolates exhibited synthesis of IAA .The ability of endophytic fungal isolates in producing hormones IAA varied from 30µg/ml to 260µg/ml. One of the strain CRP2 produced higher amount of IAA which was 260µg/ml whereas Cladosporium produced lower amount which was 30 µg/ml.

Keyword: Endophytic fungi, Cyanodon dactylon, Indole acetic acid.

Department of Botany, Deogiri College, Aurangabad. Page 232

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L-52

Diversity of endophytic fungi from Dalbergia sisso.

Dipali B. Tribhuvan*, Aparna S.Taware and Ravi P.Patil

ABSTRACT

Endophytic fungi isolated from leaves and stems of Dalbergia sisso were studied to determine the total diversity of endophytes. leaves and stems samples were collected from 8 different locations of Aurangabad region. Total 135 endophytes were isolated. Colonization frequency and dominance of these 135 endophytes were calculated out of which, five endophytes were dominant i.e. Phoma sp. (11.11%), Alternaria sp.(9.63%), Aspergillus flavus (7.41%), Aspergillus sp.3 (7.41%)and Hbl2(UI) (14.81%). Inpresent investigation, Hbl2 (UI) showed highest colonization frequency and dominance in the plant.

Keywords:Dalbergia sisso, Endophytes, Dominance, Colonization frequency.

Department of Botany, Deogiri College, Aurangabad. Page 233

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L-53

Antifungal activity and phytochemical analysis of ethyl acetate extract of endophytic fungi isolated from Citrus limon.

Manisha R. Survase* and Santosh D. Taware Mahatma Gandhi Mission, Institute of Biosciences and Technology, Aurangabad.

ABSTRACT

Twenty-two endophytic fungi were isolated from leaves of Citrus limon. The antifungal activity of endophytic fungi isolated from Citrus limon was tested against plant pathogenic fungi Alternaria alternata, Penicillium notatum, Fusarium oxysporum and Trichoderma viride by agar well diffusion method. Endophytic fungal extract showing highest antifungal activity was used for molecular characterization and phytochemical analysis. Cladosporium cladosporioides was identified after BLAST analysis which showed maximum zone of 30 mm against Alternaria alternate followed by Trichoderma viride (20 mm), Penicillium notatum (16 mm) and Fusarium oxysporum (15 mm). Phytochemical screening of extract revealed the present of alkaloids, flavonoids, glycoside, terpenoid, phenol, carbohydrate and phenolic compounds.

Keywords – Citrus limon, Endophytic fungi, Antifungal activity, Phytochemicals.

Department of Botany, Deogiri College, Aurangabad. Page 234

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L-54

ALGAL FLORA OF MAIZE FIELD SOIL IN AHMEDNAGAR DISTRICT OF MAHARASHTRA. Milind J. Jadhav and Swati G. Wagh*

Department of Botany, Sir Sayyed College, Roshan Gate area, Aurangabad-431001 (M.S.) India. *Department of Botany, Shri. Baneshwar Arts, Commerce and Science College, Burhannagar, Tal.And Dist.- Ahmednagar-414002 (M.S.) India.

ABSTRACT

Soil algae are one of the significant components of soil microflora. They play an important role in fertility of soil. Blue green algae fixes atmospheric nitrogen and increases the fertility of soil. The cultivated field ecosystem provides a favourable environment for the growth and development of algae. Present research work deals with the study of algal flora of Maize (Zea mays L.) field, located in Shrirampur tehsil area of Ahmednagar district of Maharashtra. In order to study algal flora of maize field, algal samples from moist places of field were collected at regular intervals from July 2017 to October 2017. Bold’s basal medium was also used to culture algae from soil of maize field. Collected and cultured algal samples were observed with the help of standard literature on algae. A total of 47 species under 29 genera belonged to Chlorophyceae, Bacillariophyceae and Cyanophyceae were recorded. Cyanophycean algal taxa were found dominant in the soil of maize field. Gloecocystis gigas, Gloeocystis major, Chlorococcum humicola, Chlorella vulgaris, Nitzschia palea, Aphanothce nidulans, Aphanothece saxicola, Oscillatoria obscura, Phormidium jenkelianum, Phormidium molle, Phormidium usterii, Microcoleus acutissimus, Microcoleus lacustris and Plectonema gracillimum were found abundant. Algal flora of maize field is rich and it is in diverse form.

Key words: Algal flora, maize field, soil.

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Microalgal Response to Gamma Radiation and Heavy Metals Kulkarni S.G.1*, Pawar S.T.2 and Puranik P.R.3 1Department of Biophysics, Govt. Institute of Science, Aurangabad 431004, MS, India 2Department of Microbiology, Tuljaram Chaturchand College, Baramati 413102, MS, India 3School of Life Sciences, KBC North Maharashtra University, Jalgaon 425001, MS, India

ABSTRACT

Interaction between biology and radiations has been a research area since long time. Among the various radiations, ionizing radiations are known to cause lethal effects on biological system. Higher doses of gamma rays have been extensively used for the sterilization of food and other materials. However, the low dose of gamma irradiation may not be lethal. Various cells show different mechanisms to deal with low doses of gamma irradiation. In recent years low dose of gamma has gained importance in improving microbial strains. Photosynthetic cyanobacteria are most primitive entities and bear capacity to develop various ways to cope up with abiotic stresses; both ionizing as well as non-ionizing radiations and heavy metals. The present study was aimed to understand the interactions between cyanobacteria and abiotic stresses mainly due to gamma radiation and heavy metals. Cyanobacterial cultures were exposed to various doses of gamma (20, 40, 60, 80 and 100 Gray). The growth parameters such as dry weight and chlorophyll content were checked for gamma exposed cultures. In each batch 3 tubes each of 2 cultures (total 6) were exposed to gamma source for 10 min (15 Gy). On exposure the cultures were inoculated in BG-11 medium and kept for incubation at 25C under illumination of fluorescent tubes in cyanobacterial laboratory. Cultures were further tested for their tolerance to cadmium, zinc, copper, chromium, iron and manganese (0-20 mg/l). The tolerance level differed with type of metal and culture. Microlalgal cultures viz. Chlorella sp., Spirulina sp NMU-101, Oscillatoria claricentrosa NMU-102, Synechococcus sp. and Synechocystis sp. NMU-177 exhibited highest tolerance for Cadmium, Zinc, Copper, Manganese, Chromium and Iron. Zinc, manganese and iron increased the tolerance of microalgae to gamma radiation when irradiated in presence of metals. The results indicated that heavy metals help in increasing the tolerance of microalgae to gamma radiation.

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SEASONAL VARIATIONS OF PHYTOPLANKTON IN AMBALWADI RESERVOIR AT PARLI – V.

Munde V.R.

Late, Laxmibai Deshmukh Mahila College, Parli- V.

ABSTRACT

Present investigation deals with the qualitative information on seasonal variations of phytoplankton in Ambalwadi reservoir at parli –Taluka. Phytoplankton plays an important role as primary production i.e. food for herbivorous animals. Phytoplankton acts as biological indicators of water quality and its pollution. The present investigation was carried out on Ambalwadi reservoir for period of one year from June 2017 to may 2018. The samples are collected from the surface water by filtering 100 liters of water through plankton net having a mesh size of 30 m. during investigation18 species of Phytoplankton’s belonged to four classes were identified.Phytoplanktons are representing microscopic algal communities of open water as a major element in aquatic biota. Phytoplankton is the pioneer of an aquatic food chain. The productivity of an aquatic environment is directly correlated with the density of phytoplankton. The population of Phytoplankton in any aquatic system is biological wealth of water for fishes and constitutes a vital link in the food chain. Algae contribute a part of the food chain in the aquatic environment and any change in its growth and composition will alter the other living sources including the fishes. Phytoplankton response immediately to the surrounding changes and hence it indicates the water quality. Aquatic environment supporting Hydrophytes vegetation is endowed with luxurious growth of algae. Considerable work has been carried out in India about systematic, survey, distribution, Periodicity, and ecology of algae in several habitats. The density of plankton in a water body determines the stocking rate of fishes because they are the chief source of food of many economically important fishes plankton, due to its key role in ecosystem of the environment, is directly related to the fish catch potential of a reservoir. Phytoplankton plays an important role in the biosynthesis of organic matter in an aquatic ecosystem. It serves for all living organism of water body as food of fishes. Phytoplankton also plays key role in the ecosystem of the environment. The healthy aquatic ecosystem depends on the abiotic properties of water and the biological diversity of the ecosystem (Harikrishananet.al. 1999). The Phytoplankton and Zooplankton are always inversely proportional in an aquatic environment because the Zooplankton feed on the Phytoplankton.

Keywords: -Phytoplankton, Water quality, Seasonal variations, Ambalwadi reservoir.

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Phytoplankton Diversity of various lakes in Aurangabad City, (MS), India. 1. Yogita L. Padme 2. Kshama S. Khobragade 1Dept. of Environmental Science,Dr. Babasaheb AmbedkarMarathwada University,Aurangabad- 431001, M.S., India . 2S.B.E.S. College of Science, Aurangabad - (431001), M. S, India

ABSTRACT

Phytoplankton study provides a relevant and convenient point of focus for research on the mechanism of eutrophication and its adverse impact on an aquatic ecosystem. Algal flora constitutes a vital link in food chain whose productivity depended on the quality of water at a given time. The planktonic study is a very useful tool for the assessment of water quality in any type of reservoir which contributes to understand the basic nature and general economy of the lake.Aurangabad city, a district in Maharashtra State, is one amongst the fastest growing cities in Asia. Aurangabad is one of the cities from Marathwada region with historic significance where the oldest lakes like; Nehru Lake, Harsul Lake and Salim Ali Lake are placed.Salim ali lake supplied water under the ancient water supply scheme of ‘Nahar-e-Ambari’. Originally, the area of the pond was 54 acres. It reduced to 34 acres when it lost 20 acres in the construction of Aurangabad-Jalgaon road.Nehru Lake is situated in Nehru Udyan of N-8 CIDCO area, which is one of the oldest lakes in this historical city. The Harsul Lake is located North-East of the Aurangabad city. It is 2 km away from Harsul jail. Catchment area of Harsul lake is 16054 Acers. It was constructed in 1954 and inaugurated on 5th June 1956. Water supply by Harsul Lake to Aurangabad city is about 1,00,0000 MCD.In present research work the diversity of phytoplankton, their distribution and abundance in Nehru Lake, Harsul Lake and Salim Ali Lake were studied. It is observed that, Bacillariophycea, Euglenophyceae and Dinophyceae were found maximum at all the three lakes. This indicates all the three lakes were polluted by organic pollution Keywords: Algae, Phytoplankton diversity, water body, abundance.

Department of Botany, Deogiri College, Aurangabad. Page 238

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Integrated management of Gloeosporium ampelophagum causing Anthracnose of grapes.

S.S. Chavan and V.C.Khilare. Department of Botany S.G.R.G Mahavidyalaya, Paranda, Osmanabad-413502 Department of Botany, Vasantrao Naik Mahavidyalaya, Aurangabad- 431003.

ABSTRACT

Grape is one of important cash crop grown in our country. India is one of the major grape and wine producing country in world. In India, Maharashtra ranks first in grape production as well as in grape cultivation. Grapes are affected by various fungal diseases. Along with powdery mildew and downy mildew, anthracnose of grapes is also one of the major and severe problem to the grape cultivatiours. Anthracnose of grapes is caused by Gleosporium ampelophagum. It is a serious disease in orchards of grapes in Maharashtra State. Altogether 37 isolates were collected from different grape growing districts of Maharashtra to study the morphological variation. All the isolates were screened with different fungicides to check the fungicide resistance. To break the fungicide resistant various biocontrol agents were also used for study. Biocontrols like Gliocladium virens, Trichoderma atroviride -1, T. atroviride -2, T. atroviride -3, T. atroviride-4, T. harzianum, T. koningii, T. pseudokoningii, T. viride, Chaetomium globusum, Penicillium notatum,Penicillium spp. were used in integrated management. Among them T. koningii was found to be effective in both in vitro and in vivo conditions . Gliocladium virens was also effective. Other biocontrol agents used in this study were less effective and gave PCE less than 50. Suitable biocontrols were used for the control of G. ampelophagum in laboratory as well as in field condition.

Department of Botany, Deogiri College, Aurangabad. Page 239

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Effect of Fungicides on seed borne mycoflora of mung bean (Phaseolus aureus Roxb.)

Lakde H. M.*, Aithal S. V. 1

Department of Botany, Degloor College Degloor. Udgir road Degloor Tq Degloor Dist Nanded-431717 Department of Botany, V.Dhunda Maharaj Degloorkar College Degloor Near Government Hospital, Degloor Tq Degloor Dist Nanded - 431717

ABSTRACT Seed samples of mung bean seeds were collected from local area and tested for seed mycoflora by Agar plate method and Blotter method. Twelve fungal species by Agar plate method and fifteen fungal species by Blotter paper method have been isolated. The fungi isolated were Alternaria alternata, Aspergillus candidus, Aspergillus flavus, Aspergillus fumigatus, Rhizopus stolonifer, Colletotrichum capsici, Rhizoctonia solani, Penicillium notatum, Fusarium moniliforme, Cladosporium cladosporoides, Curvularia lunata, Chetomium globosum, Macrophomina phaseolina, Fusarium oxysporum and Phoma exigua. The effect of six fungicides i.e. Thiram, Captan, Mancozeb, Vatavax, Carbendazim and Thiophenate mythel was treated on seed mycoflora of mung bean. Thiram was found to be most effective as compared to other fungicides.

KEY WORDS: Seeds of Mung bean, Mycoflora, Fungicides.

Department of Botany, Deogiri College, Aurangabad. Page 240

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Isolation of seed borne mycoflora of Pigeon pea and its in vitro evaluation by some known bioagents

Aithal S.V. 1, Lakde H.M. 2 and Sherikar V.G. 2

1 Dept. of Botany, V.D.M.D.College, Degloor (M.S.) 2 Dept. of Botany, Degloor college, Degloor (M.S.)

ABSTRACT

Due to hazardous effect of chemical fungicides, search for safer alternative to control the pathogen is better choice. This led to trials on the use of bioagents to control the pathogen. In the present study in vitro antagonistic activity using the dual culture technique and paper disc method was undertaken to assess the potential of Trichoderma harzianum, Trichoderma viride and Pseudomonas fluorescens. It showed inhibitory effects on some common pathogens of Pigeon Pea namely Aspergillus flavus, Aspergillus fumigates, Alternaria alternata Fusarium oxysporum M. Phaseolina Rhizoctonia solani. The test antagonists grow faster than the pathogen and produced inhibition zones thereby limiting the growth of the pathogen. Maximum growth inhibition of Fusarium oxysporum (81.8%), Macrophomina phaseolina (84.2%) Aspergillus flavus (74.53%) and Aspergillus fumigatus (77.77%) were found with the use of Trichoderma harzianum in dual culture and P.fluorescens in paper disc. In in vitro tests, Trichoderma harzianum, Trichoderma viride and Pseudomonas fluorescens showed significant differences in inhibiting the colony growth of the said fungal pathogens.

Key-Words: Antagonistic activity, Trichoderma harzianum, Trichoderma viride and Pseudomonas fluorescens, pathogens

Department of Botany, Deogiri College, Aurangabad. Page 241

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ISOLATION, CHARACTERIZATION AND ANTIBACTERIAL PROPERTY OF PHYCOCYANIN Shaikh Sarwar C.,Wadekar V. N.,Kulkarni S.G.

Department of Biophysics, Government Institute of Science, Aurangabad.431004 (M.S.) ABSTRACT The present investigation aimed to extract phycocyanin from microalgae spirulina plantesis, collected from Lonar Lake. The isolation of phycocyanin is achieved by enriching the biomass in modified Zarrouk’s medium at pH 8.6. The crude extracted was treated with lysozyme and purified by ammonium sulphate precipitation.Phycocyanin isolated was confirmed by its absorption peak at 615 nm and by chromatographic fractionation.The Phycocyanin extracted,was screened for antibacterial spectrum against various bacterial strains explores its ability as a drug as well as nutrient.

Key words: Phycocyanin, Spirulina plantensis, Antibacterial activity, modified Zarrouk’s medium

Department of Botany, Deogiri College, Aurangabad. Page 242

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L-62 ISOLATION, CHARACTERIZATION AND ANTIBACTERIAL PROPERTY OF PHYCOCYANIN Shaikh Sarwar C.,Wadekar V. N.,Kulkarni S.G.

Department of Biophysics, Government Institute of Science, Aurangabad.431004 (M.S.) ABSTRACT The present investigation aimed to extract phycocyanin from microalgae spirulina plantesis, collected from Lonar Lake. The isolation of phycocyanin is achieved by enriching the biomass in modified Zarrouk’s medium at pH 8.6. The crude extracted was treated with lysozyme and purified by ammonium sulphate precipitation.Phycocyanin isolated was confirmed by its absorption peak at 615 nm and by chromatographic fractionation.The Phycocyanin extracted,was screened for antibacterial spectrum against various bacterial strains explores its ability as a drug as well as nutrient.

Key words: Phycocyanin, Spirulina plantensis, Antibacterial activity, modified Zarrouk’s medium

Department of Botany, Deogiri College, Aurangabad. Page 243

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FUNGAL DISEASES OF SOME TREES FROM LONAR, DIST. BULDHANA ( M.S.) INDIA

NAVALSINGH J. TODAWAT AND P.B.PAPDIWAL

Moreshwar Arts, Science and Commerce College, Bhokardan, Dist-Jalna M.S.-431114.

ABSTRACT

The fungal diseases appear in the monsoon season in the Lonar forest range. During this surveyed, 08 plants found to be infected by fungal pathogens leading to foliage diseases, the plants were viz. Azadirachta indica, Annona squamosa, Bauhinia variegata, Cocos nucifera, Justicia adhatoda, Musa spp., Tectona grandis, and Terminalia spp. On this host plants, The species of fungi viz. Alternaria alternata,ciliochorella indica, chaconia tectonae, colletotrichum psidii, elsinoe annonae, pestalotia versicolor and phyllachora bauhiniae. The symptoms and season of occurrence were noted in the field itself.

KEY WORDS: Fungal diseases, symptoms, Alternaria, Maharashtra

Department of Botany, Deogiri College, Aurangabad. Page 244

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Induction of PR-Protein in Tomato against Fusarium wilt by using Biotic and Abiotic Elicitors.

Mustafa M. Dandu and Sopan B. Chavan

Department of Botany Arts, Science and Commerce College, Badnapur Dist. Jalna (MS) India-431202

ABSTRACT

Tomato is one of the most important fruit vegetable crop in the world, but biotic and abiotic stress are major constraint in production, due to these stress huge losses in production as well as productivity of tomato in world was observed. Fusarium wilt of tomato is soil born disease caused by Fusarium oxysporum sp. lycopersici . Tomato plant have inbuilt resistance capacity against pathogen. PR-protein and Phytoalexin is inbuilt resistance in plant, Present studies focused on visual screening Resistant and susceptible cultivar of tomato against Fusarium wilt disease and its correlation with PR-Protein from the infected field of tomato, it was observed that in Sucesptble and resistant cultivar of tomato showed level of PR-Protein and Phytoalexin are directly positive correlated to disease incidence. So it is important to induction of PR-Protein and Phytoalexin level by using elicitors.

Keyword:- Tomato, Fusarium oxysporum sp. lycopersici , Disease incidence, PR-Protein, Elicitors etc.

Department of Botany, Deogiri College, Aurangabad. Page 245

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Foldscopic Studies of the fungal endophytes of the anticancer plants N.M.ghangaonkar Department of Botany, C.T.Bora College, Shirur

ABSTRACT

Foldscope is new low-cost paper microscope developed by a team led by Manu Prakash, an Assistant Professor of Bioengineering at Stanford School of Medicine,USA. It can be assembled from a punched sheet of cardstock, a spherical glass lens and few Magnetic couplers. cost less than US$1 to build. It is affordable microscope that would be versatile and sturdy enough to work in field conditions. A Light Emitting Diode along with battery that powers the LED is an accessory. Smart phones can be attached with it to take photographs of the microorganisms.The Foldscope weighs 8 grams and comes in a kit that provides magnification at 140X. In present study compound microscope is replaced by foldscope to study the fungal endophytes of anticancer plants. The isolation and identification of the fungal endophytes was done by the foldscope and found that Aspergillus flavus, fusarium oxysporum, Alternaria terrus Aspergillus niger, Mycelia sterilia, Aspergillus Phomopsis sp. Penicillium, Aspergillus sp. Cladosporium sp.Mycelia sterilia, Aspergillus sp. Cheatomium sp. endophytes Were isolated from Solanum virginianum, Murraya koenigii , Oscimum sanctum , Adathoda vasica, Curcuma longa, Catharanthus roseus, Nothapodytes nimmoniana anticancer plants.

Department of Botany, Deogiri College, Aurangabad. Page 246

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Population Dynamics of soil mycoflora of red rot infected sugarcane field incited by Colletotrichum falcataum

Archana R. Mukhedkar1 and U.N.Bhale*1

1Department of Botany,Shikshan Maharshi Dnyandeo Mohekar Mahavidyalaya Kalam Dist. Osmanabad 413507,Maharashtra, India 2Research Laboratory, Department of Botany, Arts, Science and Commerce College, Naldurg, Tq. Tuljapur, Osmanabad 413602,Maharashtra, India

ABSTRACT

Soil is a complex ecosystem has physicochemical parameters that hold enormous number of living organisms. The present study deals with the in soil fungal population of traditional sugarcane field in Maharashtra and region. Soil fungi in sugarcane field need to improve knowledge of diversity of these micro fungi in sugarcane growing area .The mycoflora were isolated by using soil dilution technique and soil plate technique on Potato Dextrose Agar and supplemented by suitable antibiotic such as penicillin and streptomycin. Identification andcharacterization of the mycoflora were made with the help of authentic manuals of fungi. Different species belonging to Ascomycetes, Phycomycetes and Deuteromycetes were isolated. Ten samples of rhizospheric soil were collected from different localities & varieties.The dominant species i.e. Aspergillus niger Aspergillus flavus, Aspergillus fumigates , Aspergillus nidulans, Aspergillus terreus, Penicillium chrysogenum, Penicillium notatum, Trichoderma viride, Trichoderma harzianum, Fusarium oxysporum, Fusarium solani,Curvularia clavata, Curvularia lunata, and Rhizopus stolanifer were isolatedand characterized percent frequency of the mycoflora were statistically analyzed. Key words: RhizosphereSoil, Fungal population, sugarcane,Colletotrichum falcataum, dilution plate technique

Department of Botany, Deogiri College, Aurangabad. Page 247

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STATUS OF ARBUSCULAR MYCORRHIZAL FUNGAL (AMF) ASSOCIATION IN CHRYSANTHEMUM SPECIES

R.S.Narayankar1 , V.R.Kumbhar, and U.N.Bhale2*

1Dept of Botany, D.B.F. College of Arts & Science, Solapur-413002,Maharashtra 2Research laboratory, Department of Botany Arts, Science and Commerce College Naldurg, Tq. Tuljapur. Dist. Osmanabad-413602, MS, India ABSTRACT

Chrysanthemum is a perennial herb is an ornamental plant of Asteraceae with great variety flowers having different colours of cultivars of the same through out the world along with some edible and much more medicinal uses. Arbuscular mycorrhizal fungal (AMF) having present in cortical cells of different plants which increases the minerals like phosphorus absorbing capacity of plants is also associated. Chrysanthemum species found showing different types of root colonization (89%) such as root colonization like arbuscles, vesicles and hyphal types are observed. and a variety of AM fungal spores. Study carried out the large spore density (1052/100g soil) and AM fungal propogules are Glomus and Acaulospora species.

Department of Botany, Deogiri College, Aurangabad. Page 248

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Antimicrobial activity of crude extracts of endophytic fungi isolated from Ventilago denticulata *Rahul K. Dhabale*, Bhagwan M. Waghmare and Suvarna T. Kadam *Department of Botany, Bhagwantrao Arts& Science College Etapalli Dist. Gadchiroli* Botany Research Centre, Department of Botany, Maharashtra Mahavidyalaya, Nilanga. Dist. Latur. Maharashtra (India)

ABSTRACT

Endophytic fungi are eukaryotic organisms that live inside plant tissues with symbiotic association. Recently they have been recognized as a valuable source of novel bioactive metabolites for Biotechnological importance as antibiotics, anti-oxidants, anti- cancerous compounds, enzymes, vitamins, texturing agents and pigments. Therefore, different parts of Ventilago denticulata were subjected to the isolation of endophytic fungi such as Trichoderma koningiiand Fusarium oxysporum were screened for antimicrobial activity against Bacillus cereus, Staphyllococcus aureus, Candida albicans, Pseudomonas aeruginosa and Escherichia coli. Also employed for the production of secondary metabolites, which are extracted withethyl acetate and screened for antimicrobial activity by Agar well diffusion method. The results are indicating that, the extract of Trichoderma koningiifound to be effective for maximum inhibition of the Pseudomonas aeruginosa followed by Bacillus cereus, Staphyllococcus aureus and Escherichia coli except Candida albicans. The extract of Fusarium oxysporum proved their maximum inhibition activity against Bacillus cereus followed by Candida albicans, Staphyllococcus aureus and Escherichia coli. except Pseudomonas aeruginosa.

Department of Botany, Deogiri College, Aurangabad. Page 249

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STUDY OF PHYLLOSPHERE FUNGI OF SOME ORNAMENTAL

PLANTS

Mahadik S.C and Linge A.S

Ramkrishna Paramhansa Mahavidyala,Osmanabad., Venkatesh Mahajan Senior College,Osmanabad.

ABSTRACT

The present investigation was performed to observe the phyllosphere fungi of some ornamental plants.The leaf surface is exposed to air currents .With the air currents certain microscopic objects are carried to leaf surface. Ornamental plants are commercially important grown in either open field or under glass house condition. .Fungi are found every where, leaf surface provides a suitable environment for microbial growth. Phyllosphere fungi where isolated from Hibiscus rosa sinensis and Ixora coccinea , fungal species isolated were Aspergillus ,Curvularia and Fusarium .

Key words: Ornamental plants ,phyllosphere,fungi

Department of Botany, Deogiri College, Aurangabad. Page 250

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Allergenic fungal spore monitoring in some intramural sites of Aurangabad city (MS). Rajurkar S.K. & Nagwanshi M. N. Department of Botany, Deogiri College Aurangabad ABSTRACT Intramural environment provides congenial atmosphere for allergens as well as microorganisms. Their Contribution are more than outside due to low temperature and moist humid climate. For this study experiment was carried out from 1st Jan 2015 to 31st Dec 2015with the help of samplers and petriplate expose method at five places of Aurangabad city. Altogether 26 fungal types were recorded out of which 08 are allergenic, 08 are deteriorating while remaining are saprophytic fungi.16 species of 08 genera are potentially allergenic. Alternaria alternata, A. tennis, Aspergillus fumigatus, A. flavus, A. niger, Cladosporium tenuissimum, C. cldosporioids and Chaetomium globosum, Chaetomium cellulolyticare found to be dominant allergens.

Key wards: Allergens, Aspergillus, Deteriorating fungi, etc.

Department of Botany, Deogiri College, Aurangabad. Page 251

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Antimycotic Activity of Plant Extracts on Fusarium oxysporum f.sp. udum causing wilt Disease in Pigeonpea [Cajanus cajan (L. ) Mil.sp.]

V. Jalander and B.D. Gachande* Department of Botany, Telangana University, Nizamabad (T.S.) * Department of Botany, N.E.S. Science College, Nanded (M.S.)

ABSTRACT

Pigeon pea (Cajanus cajan (L.) Millsp.) is one of the major legume crop grown in the semi arid tropics. It is second most important food legume of India. It is cultivated in about 50 countries of Africa, Asia and the America, variety of uses like food, fodder, fuel wood, rearing lac insects, hedges, wind breaks, soil conservation, green manure, roofing and so on. Besides this, cultivation of pigeon pea suffers from many diseases among them wilt disease caused by Fusarium oxysporum f.sp. udum is very serious. Wilt disease is an important soil borne disease of pigeon pea causing significant reduction in yield.Plants are act as a potential source for secondary metabolites, such as flavanoids, terpenoids, alkaloids, tannins, phenols, steroids etc.In the present study, the pathogenic fungus was isolated from infected roots of pigeon pea plants. The in vitro efficacy of different plant leaf extracts (aqueous and methanolic) prepared from different plants such as Artabotrys odoratissimus R. Br., Adhatoda vasica Ness., Emblica officinalis Gaertn., Calotropis gigantia (L.) R. Br., Withania somnifera (L.) Dunal., Punica granatum L. Dalimb., Azadirachta indica A. Juss., Passiflora idulis Sims., Ipomea cornia Jacq. and Ocimum sanctum L. were tested for their antimycotic activity to control wilt pathogen of pigeon pea. Different concentrations 5, 10, 15 and 20% (aqueous and ethanolic) of plant extracts were used in this study. All the plant extracts showed significant reduction in the growth of pathogen. Among the different extracts 20% of Azardiachta indica was found most effective followed by Calotropis gigantia, and Ipomea cornia.

Key words: Wilt disease, Pigeon pea, Plant extracts.

Department of Botany, Deogiri College, Aurangabad. Page 252

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Allergenic fungal spore monitoring in some intramural sites of Aurangabad city (MS). Rajurkar S.K. & Nagwanshi M. N. Department of Botany, Deogiri College Aurangabad

ABSTRACT Intramural environment provides congenial atmosphere for allergens as well as microorganisms. Their Contribution are more than outside due to low temperature and moist humid climate. For this study experiment was carried out from 1st Jan 2015 to 31st Dec 2015with the help of samplers and petriplate expose method at five places of Aurangabad city. Altogether 26 fungal types were recorded out of which 08 are allergenic, 08 are deteriorating while remaining are saprophytic fungi.16 species of 08 genera are potentially allergenic. Alternaria alternata, A. tennis, Aspergillus fumigatus, A. flavus, A. niger, Cladosporium tenuissimum, C. cldosporioids and Chaetomium globosum, Chaetomium cellulolyticare found to be dominant allergens.

Key wards: Allergens, Aspergillus, Deteriorating fungi, etc.

Department of Botany, Deogiri College, Aurangabad. Page 253

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SEASONAL VARIATIONS OF PHYTOPLANKTON IN AMBALWADI RESERVOIR AT PARLI – V.

Dr. Munde V.R. Late, Laxmibai Deshmukh Mahila College, Parli- V. [email protected]

ABSTRACT

The present investigation deals with the qualitative information on seasonal variations of phytoplankton in Ambalwadi reservoir for period of one year from June 2018 to may 2019. The samples are collected from the surface water by filtering 100 liters of water through plankton net having a mesh size of 30 m. during investigation 15 species of Phytoplankton’s belonged to four classes were identified. This samples are collected from the surface water by filtering 100 liter of water through plankton net having mesh size of 30 m.

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L-84

Comparative studies of Synechocystis salina under different physical and chemical parameters. 1R.A Bagwan, 2K.D. Gopale, 3A.N Salve

2Department of Botany Modern college of Arts, Science and Commerce, Shivajinagar Pune 05 1 & 3Department of Botany Government Institute of Science, Aurangabad Nipat Niranjan Nagar, Aurangabad Caves Road, Aurangabad- 431004(M.S.) INDIA ABSTRACT

The experiment was perform to evaluate culture and growth performance of genus Synechocystis salinaWislouch. Two different growth media i.e. Natural Sea water and f/2 were selected for the comparision.The culture flask containing 250ml media were inoculated 50ml of anexic culture of Synechocystis salina. The culture flask were incubated at 24 ± 2 0c for 16 hours light and 8 hours dark photoperiodFive different pH of the media ranging from 5 to 9 was maintained. The growth rate of Synechocystis salinawas analyzed by cell count method with the help of haemocytometer at an interval of 48 Hours. At the end of 17 days of maximum growth was achieved in f/2 media at pH7 (172 cells/ml) and sea water media at pH 6 maximum growth was achieve at pH 6 on 11 days. (60.6 cells/ml) thus from the culture it is clear that the influence of chemical compound on the growth Synechocystis salinais clearly observed.

Department of Botany, Deogiri College, Aurangabad. Page 255

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L-85

STUDIES ON POWDERY MILDEW OF SOME FOREST PLANTS IN GAUTALA FOREST, KANNAD, INDIA Kantule A.P.and Pawar S.M.*. Research guide, Department of Botany, Shivaji college , kannad (M.S.) Corresponding author’s email: [email protected]

Abstract The leaf samples of the plant ficusreligosa and Euphorbia heterophyllawere collected in phytopathological survey of forest plants. The survey showed the presence of fungal disease Powdery mildew on many plants . . In phytopathological survey from septeber –november 2016, showed the presence of fungi Erysiphae that causes powdery mildew on some tropical trees of forest. Detailed description ,illustrations and comments are provided

Keywords: Phytopathological survey, erysiphae, Gautala forest , India

Department of Botany, Deogiri College, Aurangabad. Page 256

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L-86

ENZYMATIC ACTIVITY OF FUNGI ASSOCIATED ON FRUIT ROT OF COCCINIA INDICA (WIGHT AND ARN).

V. S. Chatage and U. N. Bhale

Department of Botany, Kai. Rasika Mahavidyalaya, Deoni, Tq. Deoni, Dist. Latur,- 413519, (M.S.) India.Research laboratory, Dept. of Botany, Arts, science and Commerce College Naldurg, Tq. Tuljapur Dist.Osmanabad – 413602 (M.S.)[email protected],

ABSTRACT

Enzymatic activity e.g. cellulose, amylase, lipase by cup plate and pectinase enzyme by viscomertric method of fungal pathogens causing Ivy gourd fruit rot disease in rot was determined. In- vitro studies; fruit rot pathogens viz., Macrohomina phaseolina and Geotrichum candidus. M. phaseolina showed maximum cellulose activity (26mm). Maximum cellulase activity was showed by G. candidus (30mm). Amylase activity was also maximum enzymatic activity of M. phaseolina (35mm). However, G. candidus (20mm) had a little significant amylase activity. Also recorded significant lipase activity followed by M. phaseolina showed maximum lipase activity (30mm). Amylase activity was showed inG. candidus (18mm)and Pectinase, The extracellular pectinase activity by M. phaseolina (89.94 %) and G. candidus (74.30%) activity after 30mins.

Key words: Coccinia indica, Macrohomina phaseolina and Geotrichum candidus cup plate method, enzymes ect.

Department of Botany, Deogiri College, Aurangabad. Page 257

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L-87

Standardization of Mushroom Cultivation on

Agro-wastes in Marathwada Region

D.K. Kirwale and D.S. Mukadam Dept. of Biology,VivekanandCollege,Aurangabad (M.S.). *Ex.Prof. & Head, Dept. of Botany, Dr. B.A.M.U.Aurangabad (M.S.)

ABSTRACT Mushroom Cultivation Technology at present is highly developed both for better nuturitional values and high yield. Commercially mushroom cultivation has been found viable in all respects. Mushroom cultivation is an essential and very much useful attempt for recycling a large quality of agro- wastes. Mushrooms are expected to provide proteins to ever increasing population in future. Marathwada region has a great potential due to favorable wheather conditions and labour. Mushroom cultivation is gaining momentum world wide because of its excellent nutritional value. It provides a protein-rich low caloric diet and has beneficial effects on heart patients. Oyster mushroom pleusotus are fruiting bodies of fungi belonging to group Basidiomycotina they are found to be very good source of balanced diet. between meat and vegetables. It can be our ideal small-scale industry for gainful employment.

Keywords: Agro-wastes, oyster mushroom, Marathwada region.

Department of Botany, Deogiri College, Aurangabad. Page 258

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Theme-M

Taxonomy and Plant Systematic

Department of Botany, Deogiri College, Aurangabad. Page 259

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M-1

SOME NEW EXOTIC PLANT RECORDS TO THE FLORA OF MAHARASHTRA

Vinod Raghoji Jogdand

Department of Botany, . RFNS,Senior science college Akkalkuwa'Dist Nandurbar.(M.S.), India

ABSTRACT

During the exploration in Maharashtra, the authors has collected some species which are exotic ones. After scrutiny of the literature, N -Tissue culture biotechnology and nano technologyas Browallia speciosa Hook. (Solanaceae); klenia stapeliiformis (E.Philips) Stapf. (Asteraceae);Klenia petraeus (R.E.Fr) C.jeffrey (Asteraceae) and Corepsis auriculta L . (Asteraceae). These species are reported to be new records to the flora of Maharashtra. Present paper deals with their description, phenology and ecological notes which help to depict taxonomic identity.

Key Words: Exotic plants, new records, Flora of Maharashtra.

Department of Botany, Deogiri College, Aurangabad. Page 260

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M-2

Taxanomy and Diversity of Coriolopsis From the Nanded District of Maharashtra (M.S.) India. Raibhole U.K. Head Department of Botany, Shivneri Mahavidalaya Shirur Anantpal, Latur Marathwada India.

ABSTRACT

Coriolopsis is the genus from order Aphylophorales only valid species have been reported from India but the present study reports 3 species, the species are each described & the fruit bodies spore are illustrated.

Keyword : Aphyllophorales- Polyoraceae Marathwada, Nanded District.

Department of Botany, Deogiri College, Aurangabad. Page 261

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M-3

NUT MORPHOLOGY OF THE GENUS SCIRPUS (CYPERACEAE): SEM STUDY IN BEED DISTRICT, MAHARASHTRA, INDIA.

Sudhir Solanke*., Raffique Shaikh. ** & Ravikiran Sawant. *** Dept. of Botany, Shri Muktanand College, Gangapur, Dist. Aurangabad. *&*** Dept. of Botany Milliya College, Beed. **

ABSTRACT

Eight species of the genus Scirpus (Cyperaceae) in the study region. Nuts have been studied under scanning electron microscope to know the detailed micromorphological features. It was found that morphological characters can be grouped into two main types such as i) Nuts having trigonous and ii) biconvex, shining distinctly reticulate. It was found that 5 species having characters referable to types (i) Marshy, lands, ditches, ponds, tanks, rice- fields, along banks of water courses, and 3 species having characters of category (ii) are plants that usually grow in rare in marshy and wet grass lands, along banks of rivers and margins of tank.

Key words: Scirpus (Cyperaceae), Nuts, morphology, SEM

Department of Botany, Deogiri College, Aurangabad. Page 262

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M-5

Retaining the dominancy by local flora over foreign in Marathwada region.

Momin R. K.*

Department of Botany, Milliya Senior College, Beed. Maharashtra ( INDIA)

ABSTRACT

Parthenium hysterophorous is a flowering plant belongs to the family Asteraceae. It is native of American tropics common name include Santa, Maria Santa Maria feverfew white top weed, famine weed. In India it is commonly called as Carrot grass or Gajarghass. It is Poisonous species due to which allergic asthma, itching and different type of ailments occurs in human beings. In the present paper biological control of parthenium by Cassia uniflora species has been observed in all parts of Marathwada region in Maharashtra as retaining the dominancy by local flora over foreign.

Department of Botany, Deogiri College, Aurangabad. Page 263

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M-6

Pollen morphology of Mimosoideae family from Manchippa Reserve Forest, Nizamabad district, Telangana state.

P.Ravi Raj and A. Vijaya Bhasker Reddy

Applied Palynology Research Laboratory, Department of Botany, Nizam College (A), Osmania University, Hyderabad, Telangana State, India – 500001.

ABSTRACT

Pollen morphological studies were carried out in 23 plant species of Mimosoideae family from Manchippa Reserve Forest, Nizamabad district. The pollen characters studied are type of pollen units, size, shape, aperture and ornamentation types. Spheroidal, prolate spheroidal, euprolate, sub prolate, oblate and oblate spheroidal type shapes of the pollen grains are recorded. Aperture types noticed are inaperturate and trizonocolporate. Ornamentations found are psilate, microreticulate, granulate, verrucate, foveolate, punctuate - foveolate. Individual monad grain size of polyads helps in the identification of species. External morphology of this family shows diversity in habit such as herbs, shrubs and trees. Bipinnately compound leaves, armed and unarmed plants. Inflorescence 2 or 3 coloured or concolourous. Flowers are in racemose spikes and globose heads, stamens are definite and indefinite Pods curved/ spirally twisted or straight. The pollen morphology of Mimosoideae also shows diversity in type of grains, size, shape, apertures and ornamentation which aids in the identification of taxa and useful in taxonomy and Palynological studies of Nizamabad district in future.

Key words: Palynology, Manchippa reserve forest, mimisoideae.

Department of Botany, Deogiri College, Aurangabad. Page 264

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M-8

Common Names of Coconut (Cocos nucifera L.Arecaceae): Some Indications On Its Nativity. Patil 1D.A. and S.B.Shisode2 1.Post-Graduate Department of Botany, S.S.V.P.S’s L.K. P.R.Ghogrey Science College, -424005 (Maharashtra), India 2. Post-Graduate Department of Botany,Loknete Vyankatrao Hiray Arts, Science and Commerce College, Panchvati,Nasik- 422003 (M.S.) India.

ABSTRACT

Common or vernacular names of plants are christened in vernacular language by common peopleover a long past. These names emerge out from the mist of times and are passed by word of mouth over generations. Such names are therefore enriched with the experiences, observations and wisdom of the concerned human society. These hidden secretes are hardly earthed by the scientific community, particularly by botanists, as they have perforce been forced to follow the rules of ICBN. Coconut palm is widely distributed and hence received a vast array of names worldwide. Moreover, its nativityhas remained a subject of debate and object of many investigations they are hence analysed etymologically in this communication. Its nativity, history and migration apart from the root words for coining names are highlightened. Some hard evidences have been also comparatively discussed to lend support while arriving at its nativity as Indo-Pacific vis-a-vis Asian.

Key Words: Common names, Coconut, Cocos nucifera, Etymology.

Department of Botany, Deogiri College, Aurangabad. Page 265

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M-9

Psychoactive Plants in the State of Maharashtra (India)

D.A. Dhale* and D. A. Patil

Post-Graduate Department of Botany, SSVP Sanstha’s, L. K. Dr. P. R. Ghogery Science College, Dhule-424 005, (M.S.) India

ABSTRACT

The vegetable kingdom is a treasure-trove of biodynamic constituents and being used for sustenance of mankind. Some plant species are psychoactive occurring naturally or found under cultivation in Indian region. They are employed in psychiatry or in medicine even by aboriginal societies. Researchers have documented them but the literature is scattered in various print forms. The present account is an overview of the past works restricted to the state of Maharashtra. Total 225 plant species belonging to 174 genera and 77 families of angiosperms, gymnosperms and pteridophytes are documented for the said state a fair segment (64 species) of which is found under cultivation for various purposes. Properties and utilities of the psychoactive plants are provided for each species along with their literary sources. This overview will be useful for the research work psychiatry medicine and biodiversity planners in future.

Key Words: Psychoactive Plants, Maharashtra state

Department of Botany, Deogiri College, Aurangabad. Page 266

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M-10

TAXONOMIC STUDIES ON WEED DIVERSITY OF MARATHWADA REGION OF MAHARASHTRA STATE

R.D. Gore & V.S. Chatage*

Department of Botany, Walchand College of Arts and Science, Solapur (MS), India *Department of Botany, Kai. Rasika Mahavidyalaya Deoni, Dist- Latur (MS), India

ABSTRACT

The unwanted plant species growing in ordinary environmental conditions are popularly known as weeds. Diversity of weeds in agricultural field is mainly depend on agricultural practices such as irrigation pattern, topography, use of fertilizers, socio economic condition, spraying and diversity of habitats. Weed diversity in agricultural field having dual importance, firstly most of weeds are used a source of cattle feed and secondly various weed species are traditionally used in ayurvedic practices, in the wake of that weeds may resolves enormous loss of crop yield and low yield may directly affect on farmers economy. Now a day’s farmers are using more herbicides in crop field to control the major threat from weeds because they know weeds are dwelling the fungi, bacteria, thrips and variety of insects. Mostly the weeds are categorized in belong to the family Commelinaceae, Poaceae, Cyperaceae etc. are more dominant over the present herbicides, but they can only eradicated by following traditional practices. Generally, they are primary competitors for the crops, lawn, orchard, gardens etc. hence these are the hazardous to creating major problem which decrease the economy, hence the proposed study carried out in 2016-2019 to study weed diversity of the Marathwada Region of Maharashtra State. During the field extensive field exploration we collected the 168 weed species from Marathwada region in respect of detailed morphology, flowering and fruiting season, weed type, present occurrence, ecological notes and medicinal importance. Among them about 20 weed species are rare from agricultural fields. The weeds of the region generally classified into two categories viz. monsoon weed and autumn weeds. Weed flora is having luxurious growth with habitual diversity in monsoon season due to enough rainfall and availability of moisture in soil but in autumn they are weaker and showing less diversity. Key Words: Weed Science, Flora, Marathwada, Agriculture, Ayurveda, Maharashtra.

Department of Botany, Deogiri College, Aurangabad. Page 267

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M-14

STUDY OF SOME MEDICINAL PLANTS IN SOEGAON, AURANGABAD (M.S.) INDIA

Deepak Devilal Pardhe Department of Botany, Sant Dnyaneshwar Mahavidyalaya Tal. Soegaon, Dist. Aurangabad

ABSTRACT

Maharashtra state flora abounds in medicinal plants which can be called as storehouse as it covers varied geographical area and phytogeographical area. Soegaon is situated at 20.60 North latitude, 75.620 East longitude and 375 meters elevation above the sea level. There are a number of advantages of associated with using herbal medicines as oppose to pharmaceutical products. Medicinal plants have been identified and used throughout human history. In present investigation 15 medicinal plants were collected from a various places which are abundant in Soegaon, Aurangabad. The importance of collected medicinal plants is discussed in this investigation.

Keywords: Medicinal plants, diversity, geographical area, advantages

Department of Botany, Deogiri College, Aurangabad. Page 268

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M-15

SOME NOTEWORTHY PLANT RECORDS TO THE FLORA OF (MAHARASHTRA) INDIA

Kakpure M. R. *, More U. A and Muneshwar A.S. Assistant Professor, Department of Botany, Late R. B. Arts, Commerce & Smt. S. R. Bharti Science, College, Arni Dist. Yavatmal – 445103.

ABSTRACT Yavatmal district is situated in the eastern part of Maharashtra State between 190.28’ and 200.48’ N latitudes and 770.19’ and 790.07’ E longitudes. Botanically, the vegetation of Yavatmal district was explored by Karthikeyan S. and Anand Kumar (1993). They reported 579 species spread under 365 genera and 98 families. The Present investigation deals with the report of 03 noteworthy plant species [viz. Tecomella undulata (Sm.) Seem, Tephrosia pumila (Lam.) Pers.and Typhonium venosum (Dryand. ex Aiton) Hett. & Boyce.belonging to 03 families reported for the first time to the flora of Yavatmal district which provides the information about morphology, phenology and occurrence of 03 additional plant species for future work.

Key words: New addition, plant species, families and Yavatmal district.

Department of Botany, Deogiri College, Aurangabad. Page 269

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M-16

ANATOMICAL STUDIES IN TEPHROSIA VILLOSA (L.)PERS.

Tukaram Gitte and Arvind Dhabe*

Dept. of Botany, Vaidyanath College, Parli-Vaijnath, Dist. Beed-431515

*Dept. of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431004

ABSTRACT

Tephrosia tinctoria Pers. is a species belonging to family Fabaceae. The juice of leaves given in dropsy, fresh roots are considered hypoglycaemic. Present study deals with morphology, stem and leaf anatomy, micromorphology and maceration studies so as to standardize the species. The data will also be useful in taxonomic characters value addition

Keywords:Tephrosiavillosa, anatomy, maceration, Fabaceae.

Department of Botany, Deogiri College, Aurangabad. Page 270

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M-17

Study and Enumeration the medicinal plant of tribal areas, Nasik District

ZIYA ANSARI , Y. S. PATIL

S. P. H. Mahila Mahavidyalaya, Camp, Dist. NAshik

ABSTRACT

In the Nasik district, 21.75 % area covered by forest eastern side of Nasik such peth, surgana, Igatpuri region. During survey of this region, collecting the medicinal plants, properly enumeration and study their medicinal used. The source information was collected from tribal people, cowherds sand Shepherds. The collected information is done popper documentation.

Keyword: medicinal Plant, Nashik district

Department of Botany, Deogiri College, Aurangabad. Page 271

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M-18

MORPHOLOGICAL ANATOMICAL AND PHYTOCHEMICAL STUDIES ON CLEMATIS HEYNEI M. A. RAO.

S. T. Chavan

Rashtramata Indira Gandhi College, Jalna (M. S.) India.

ABSTRACT

Clematis heynei M. A. Rao have been used for medicine for long times. Clematis heynei is wild as well as ornamental species abundance occurring in India. These plants have been used in the indigenous system of medicine for a long time. The morphological and anatomical studies reveal that there are not able difference in the plant habit, leaves, stem, root and flowers among this plant. Morphology and anatomical study also helps for correct identification and nomenclature of plant. Anatomical studies included observation of transverse sections of root, stem, leaves and petiole to understand the nature of different cell arrangements in these parts. The chemicals present in root, stem and leaves drugs are analyzed physically as well as chemically by qualitative and quantitative parameters. In present study reveals morphology, anatomy, medicinal properties and Phytochemical studies of the plants for the correct identification, authencity of drugs. The details are presented in paper.

Key words:Clematis heynei,morphology, anatomy, medicinal uses and phytochemical.

Department of Botany, Deogiri College, Aurangabad. Page 272

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M-19

Dermatological studies of genus Alysicarpus Desv. Shantanu Chavan1, Rupali Taur & Dillep Pokle2 1Dept of Botany, Sant Dnyaneshwar Mahavidyalaya Soegaon, Dist Aurangabad. 2Dept of Botany, Dr Babasaheb Ambedkar Marathwada University, Aurangabad.

ABSTARACT

Leaves are probably the most varied organs of the angiosperm. Carlquist (1961) has reported the valuable role of leaf anatomy in taxonomy. Irvine (1961), Metcalfe and chalk (1983) reported the use of epidermal characters such as leaf surface, epidermal cell wall pattern, nature of stomata as identifiable aids of some families and genera and sometimes for species. Metcalfe and Chalk (1950) reported a diversity in stomatal types in a tribe, a genus, or a species in the papilionaceae. They mentioned a rubiaceous (paracytic) stomata in Alysicarpus. In the presently investigated Alysicarpus variants revealed three type of stomata i.e. paracytic, anisocytic, anomocytic; the epidermal cell are isodiametric, polygonal, hexagonal, rectangular, irregular and anticlinal walls were observed viz. straight, undulate, curved and slightly wavy.Shah (1969) has revealed that there is a diversity of stomata even on the same species. In the present investigation it was found that the stomata are present on Adaxial as well as Abaxial surface. Paracytic stomata is predominant type which is found in all the 41 taxa under investigation.

Keywords: Dermatological studies, Alysicarpus Desv.

Department of Botany, Deogiri College, Aurangabad. Page 273

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M-20

OCCURRENCE OF SOME COMMON METROPOLITAN TREES IN DEVELOPING CITIES OF MARATHWADA AND BIO- ASTHETIC PLANNING V.B.CHAVAN and L.K.SURYAWANSHI

P.G.Dept.of Botany Science College, Nanded. Maharashtra.

,J.J.T.U.RAJASTHAN.

ABSTRACT

The present paper reports the occurrence of some common metropolitan trees in the developing cities of Marathwada region of Maharashtra state. Out of 10 trees described 5 are reported for the first time in the urban flora of Marathwada. Some of these newly reported plants are Adenanthera pavonia Linn , Artocarpus integrifolia Linn, Diospyros malbarica (Dess) Kostel, Streculia villosa Roxb, Anthocephalus cadamba (Roxb.),Miq. And Kleinhovia hospita L., An emphasis on bio-aesthetic planning and more use of trees therein developing cities in suggested.

Department of Botany, Deogiri College, Aurangabad. Page 274

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M-21

SOME NOTEWORTHY PLANT RECORDS TO THE FLORA OF YAVATMAL DISTRICT (MAHARASHTRA) INDIA

Kakpure M. R. *, More U. A and Muneshwar A.S. Assistant Professor, Department of Botany, Late R. B. Arts, Commerce & Smt. S. R. Bharti Science, College, Arni Dist. Yavatmal – 445103.

ABSTRACT Yavatmal district is situated in the eastern part of Maharashtra State between 190.28’ and 200.48’ N latitudes and 770.19’ and 790.07’ E longitudes. Botanically, the vegetation of Yavatmal district was explored by Karthikeyan S. and Anand Kumar (1993). They reported 579 species spread under 365 genera and 98 families. The Present investigation deals with the report of 03 noteworthy plant species [viz. Tecomella undulata (Sm.) Seem, Tephrosia pumila (Lam.) Pers.and Typhonium venosum (Dryand. ex Aiton) Hett. & Boyce.belonging to 03 families reported for the first time to the flora of Yavatmal district which provides the information about morphology, phenology and occurrence of 03 additional plant species for future work.

Key words: New addition, plant species, families and Yavatmal district.

Department of Botany, Deogiri College, Aurangabad. Page 275

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M-22

SOME NOTEWORTHY PLANT RECORDS TO THE FLORA OF YAVATMAL DISTRICT (MAHARASHTRA) INDIA

Kakpure M. R. *, More U. A and Muneshwar A.S. Assistant Professor, Department of Botany, Late R. B. Arts, Commerce & Smt. S. R. Bharti Science, College, Arni Dist. Yavatmal – 445103.

ABSTRACT Yavatmal district is situated in the eastern part of Maharashtra State between 190.28’ and 200.48’ N latitudes and 770.19’ and 790.07’ E longitudes. Botanically, the vegetation of Yavatmal district was explored by Karthikeyan S. and Anand Kumar (1993). They reported 579 species spread under 365 genera and 98 families. The Present investigation deals with the report of 03 noteworthy plant species [viz. Tecomella undulata (Sm.) Seem, Tephrosia pumila (Lam.) Pers.and Typhonium venosum (Dryand. ex Aiton) Hett. & Boyce.belonging to 03 families reported for the first time to the flora of Yavatmal district which provides the information about morphology, phenology and occurrence of 03 additional plant species for future work.

Key words: New addition, plant species, families and Yavatmal district.

Department of Botany, Deogiri College, Aurangabad. Page 276

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M-23

A BRIEF SURVEY OF SOME MEDICINAL WEEDS

FROM AURANGABAD DISTRICT, MAHARASHTRA.

Sangeeta S. Sutar, Chitra Bagmar

Dept. of Botany, Sir Sayyed College, Aurangabad.

Dept. of Microbiology, Sir Sayyed College, Aurangabad.

ABSTRACT

In Ayurveda and Unani system of medicine, a number of plantsare used for the treatment of diseases. A keen observation at theseplants has shown that a number to them are weeds (an unwanted plantin a particular area) considered by agriculturist, we could also findsuch weeds in a numbers of places in our city Aurangabad. A briefreview of literature for their medicinal uses was carried out. We alsocollected the plants so as to know its dominance in a particular area.The objective of the present study was to make the people aware of themedicinal uses of these weeds which otherwise stand neglected.We collected a total of twenty weeds and studied their medicinaluses. It could also provide a source of additional income to some ofthe farmers if they understand its benefit.

Key words: - Medicinal uses, weeds

Department of Botany, Deogiri College, Aurangabad. Page 277

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M-24

MORPHOLOGICAL ANALYSIS OF SPECIES OF CHLOROPHYTUM USING NUMERICAL ANALYSIS

Smita P. Gudadhe 1, Prashant J. Gadge 2* Varsha S. Dhoran3 and Varsha N. Nathar4

1*Arvindbabu Deshmukh Mahavidyalaya, Bharsingi, Department of Botany, Tq. Narkhed, Dist. Nagpur - 441305, Maharashtra. 2*Department of Botany, Arts, Science and Commerce College, Badnapur, Dist. Jalna - 431202, Maharashtra. 3-4 Department of Botany, Sant Gadge Baba Amravati University, Amravati - 444602, Maharashtra.

ABSTRACT This study examines interrelationship between species of Chlorophytum on the basis of morphological data. In all seven species were collected from varied region of Maharashtra (India) and identified. For each sample 25 qualitative and 15 quantitative characters were scored and data were subjected to numerical taxonomy analysis using MVSP 3.22 software. All the species can be studied by clustering based on morphological characters. The result of the present study clearly indicates that there are 2 clades in phenogram. In group one three species viz. C. nimmonii, C. comosum and C. bharuchae, from these C. comosum and C. bharuchae found closely related on the other hand, second group consists of four species namely C. arundinaceum, C. borivilianum, C. laxum and C. tuberosum, in this C. arundinaceum, and C. borivilianum located on separate clade and C. laxum and C. tuberosum grouped together. Along with cluster analysis, principle component analysis (PCA) and principle coordinate analysis (PCO) was also carried out, which clearly support the result obtained by cluster analysis. Among the 40 characters studied the characters like Ch1, Ch5, Ch12, Ch13, Ch14, Ch31 and Ch37 found important in the present study.

Keywords:Chlorophytum, Numerical taxonomy, Phenogram, PCA, PCO.s

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M-25

Comparative Studies of Tribulus terrestris and T. rajasthanensis Bhandari & Sharma of Zygophyllaceae Pathak M P1 and Kulkarni G B2 Department of Botany, JES R.G. Bagdia Arts, S.B. LakhotiyaCommerceand R. Bezonji Science College, Jalna- 431203 (M.S.)

ABSTRACT A comparative study has been carried out on specimens of a number of species in the Marathwada region of Maharashtra, in India. To find out whether the Tribulus species with identical characters reported from above regions are indeed same or different in morphologically and anatomically. There is slight variation in anatomically in root, stem and leaf of Tribulus terrestris and T. rajasthanensis Bhandari and Sharma. Similarly morphologically also have some minute variation in shape, size and structure of plant organs viz leaf, stomata, flower etc. The root and stem showed the presence of calcium oxalate crystals in cortex and pith region, sclerenchymatous patch on outer side of vascular bundle. Leaf showed the presence of small vascular bundles in the lamina and large curved vascular bundle in mid rib region. Leaf showed the presence of anisocytic type of stomata and unicellular, uniseriate, unbranched, conical and pointed trichomes with swollen base.

Key words: Tribulus terrestris, T. rajasthanensis, anatomy, trichome, stomata, calcium oxalate crystals

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M-26

Campus Flora of Shri Shivaji College Kandhar of Taluka Kandhar, District Nanded, Maharashtra State, India. Rizwan Younus Khan1*, N. J. M. Reddy2, Mulani R. M.3, Vijigiri Dinesh4, Abrar Baig5 and Jadhav Arunkumar H.6 *1Ph.D. Research Student in Botany, SRTM University, Nanded. 2Professor and Principal at Shri Renukadevi College, Mahur, Dist. Nanded. 3Professor in Botany at School of Life Sciences, SRTM University, Nanded. 4Asst. Prof. in Botany, Shri Renukadevi College, Mahur, Dist. Nanded. 5Asst. Prof.(C.H.B.) in Botany, Shri Shivaji College, Kandhar, Dist. Nanded. 6Ph.D. Research Scholar in Botany at School of Life Sciences, SRTM University, Nanded.

ABSTRACT Shri Shivaji College of Arts, Commerce and Science is situated on the bank of famous lake of Kandhar “Jagatung Sagar” in Navrangpura area of Kandhar Taluka, Nanded District, Maharashtra. College compus is very beautiful and biologically diverse. Therefore it is necessary for Botanist to know and eneumerate the plants present in the college campus. The present paper deals with floristic account of flowering plants and provides a preliminary analysis of the flowering plants of Shri Shivaji College Kandhar. During present investigation a total of 177 plant species have been reported out of which 03 plant species are of three different families of Gymnosperms and 174 plant species are of Angiosperms. 174 angiospermic plants species belongs to 140 genera and 64 families.

Keywords: Flora, Campus, Kandhar.

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Theme-N

Tissue Culture Biotechnology and Nanotechnology

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IN VITRO MULTIPLICATION STUDIES IN SPILANTHES ACMELLA L. (MURR.)

Algabri Ahmad and Narayan Pandhure Tissue culture Laboratory, Department of Botany Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431001, India

ABSTRACT

During the present investigation, protocolfor in vitro shoot multiplication of medicinal plant Spilanthes acmella L. (Murr.)was developed using axillary buds. The axillary buds explants were aseptically inoculated on Murashige and Skoog (MS) with various concentration and combinations of growth hormones such as auxins and cytokinins. Multiple shoot formation was recorded on MS medium fortified with 1.0 mg /L of IAA along with various concentration of BAP. Rate of regeneration was higher using 1.0 mg /L of IAA along with 3.0 mg/L of BAP with stem node as an explant. Keyword:Spilanthes acmella, Shoot, Multiplication.

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TISSUE CULTURE AND PHARMACOGNOSTICAL STUDIES IN DATURA INOXIA MILL.

Pallavi Prashad and Narayan Pandhure **Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431001

ABSTRACT

Datura (Datura inoxia , Family- Solanaceae) is non-traditional medicinal plant. Datura inoxia has many medicinal properties like anodyne, antispasmodic, hallucinogenic, hypnotic and narcotic etc. Datura inoxia used in the treatment of insanity, fevers with catarrh, diarrhoea, scabies, piles, ulcers, colds, asthma, Cardiac disorders, Impotency, Antispasmodic, Malaria, Baldness and skin diseases. The plant contains several alkaloids, the most active of which is scopolamine. It is also useful in respiratory ailments, rheumatism, elephantiasis, insanity, ear ache and eye diseases. During the present investigations efforts have been made to establish protocol for in vitro propagation and Pharmacognostic screening of this important medicinal plant. Keywords: Datura inoxia Mill, In vitro studies Phytochemistry.

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ANTIMICROBIAL ACTIVITY OF IMPORTANT MEDICINAL PLANT ADANSONIA DIGITATA Shilpa Chavan, Ashwini Kasbe and Narayan B. Pandhure

Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad 431004, Maharashtra, India

ABSTRACT

Seed extracts of Adansonia digitata (Seed) was analyzed for their antimicrobial activities against human pathogens Salmonella typhi, Escherichia coli, Bacillus subtilis and Staphylococcus aureus as human pathogens and plant pathogens Aspergillus spp., Alternaria spp., Penicillium spp. and Fusarium spp. These pathogens were subjected to Aqueous and ethanolic extracts of seeds. The phytochemical analysis was carried out for the detection of primary and secondary metabolic like alkaloids, steroids, terpeniods, flavonoids, flavones, gallic acid, tannins, catabolic tannins, reducing sugars and while carbohydrates, glycosides, saponins, phenols, proteins and amino acids. Experimental results have been discussed here. Keyword: -Aantimicrobial, phytochemical screening and medicinal plant Adensonia digitata

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Review on GMO Technology Eco-friendly to some Aspects

Sandip R.Rathod

Katruwar Arts, Ratanlal Kabra Science & B.R.Mantri Commerce College, Dist- Parbhani.(MH)

ABSTRACT

Our General Views about Genetical modified organism technology that have some are best applications. They do not have harmful side effects, scientifically its having complicated but practically its benefited to both human being as well as environment. Some people published his work Genetically modified organisms gives us hazardous to humans and Natural environment. GMO give us significant aspect to expanding the natural biodiversity (Genetic Diversity) circuitously. We know about loss genetic diversity due to altering environment such as loss natural habitat, climate change, human interference, natural disasters, breakage food chain etc. to maintain this natural ecosystem for that purpose GMO helps to maintain the genetic diversity in environment.

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“Isolation and screening of endophytic fungi for biosynthesis of silvernanoparticles”

Sayyad Shahim1, Gachande B D1 and Shaikh N F2

1Post Graduate Department of Botany, N.E.S. Science College, Nanded. 2Government College of Arts and Science, Aurangabad. Corresponding author’s E-mail:- [email protected].

Abstract

Now a day’s area of biosynthesis of nanoparticles via myconanotechnology increasing because fungi are ideal candidates in the synthesis of metal nanoparticles, because of their ability to secrete large amount of enzymes with low expenses have been found to be capable of synthesizing nanoparticles. These myconanoparticles have its several applications in the field of medicine and agriculture. In present research we have isolated different endophytic fungi fromhost plantMaytenus emarginata (Willd.) Ding Hou.” from leaf and stem. Isolated endophytic fungi are Nigrospora sphaerica, Alanphillipsia aloeigena, Colletotrichum gloeosporioides and Fusarium brachygibbosum. The isolated endophytes were screened for biosynthesis of extracellular silvernanoparticles (AgNP) by using the mycelium free filtrate incubated with 1 mM silver nitrate solution visually observed over a period of time indicates the bioreduction of silver ions to silver nanoparticles by change in colourless filtrate to brown colour. So from above investigation it is confirmed that isolated fungi can be used for further work to its potential activity in medicine and agriculture. Keywords :- Endophyte, Maytenus emarginata, myconanoparticles & AgNP.

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N-6

IN VITRO CALLUS INDUCTION IN TERMINALIA ARJUNA

RUSHIPATNA TARE AND SANJAY BIRAJDAR* *Department of Botany, Chhatrapati Shivaji College, Omerga.

Abstract

The research aims for standardization of protocol for In vitro callus induction protocol in forest and medicinal plantsTerminalia and Maduka. Terminalia Arjuna (usually simply referred to as Arjuna) is a tree bark that is used medicinally in Ayurveda for the purposes of cardiovascular health pertaining to the heart itself. It has a large variety of bioactive, with the water extract showing promise at improving left ventricle function of the heart without any observable toxicity of side effects when taken at 500mg thrice a day (every 8 hours). There are numerous human studies conducted on Arjuna bark, although many of them are low in sample size. Nevertheless, the water extract appears to be effective in improving cardiac function in persons who have recently undergone cardiac trauma or injury; Myocardial Infarction is the most commonly researched ailment in this regard. Only one study exists on otherwise healthy persons, but Arjuna showed benefit in improving left ventricle function in an exercise test and the benefits may affect a person regardless of health state. Fragile, profuse growth of callus was recorded on MS supplemented with 1 mg/lit 2, 4-D. Key words: Terminalia arjuna, Callus,

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Detailed quantification of base metals and precious metals of scrap PCB of mobile phones by FE SEM/EDAX and Inductively Coupled Plasma-Optical Emission Spectroscopy method.

1Sonawane P.M and 2Gupta S.G.

1Department of Biotechnology, Government Institute of Science, Aurangabad. 2 Director, Government Institute of Science, Aurangabad.

ABSTRACT

With advances in mobile technology, the obsession for the latest mobile gadgets is increasing which is further acting as a major contributor towards the generation of e-waste. So, the current study was focused to UNLOCK the value of metal content of scrap mobile printed circuit boards (PCB). For this purpose, the investigative study was done using Field Emission Scanning Electron Microscopy (FESEM) to analyse the topology of 0.5 mm particle size of Electronic Scrap Material (ESM) of PCB of scrap mobile phones and Energy Dispersive X-ray Analysis Spectroscopy (EDAX) was done to assure the presence of base and precious metals in it. Later, the confirmatory study was more focused on the determination of multi-element content by Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) method. ICP OES method which is a highly sensitive and accurate method was done using 0.5 mm fraction of PCB of mobile phones. The study revealed, the presence of base metals Co < Mg < Pb < Zn < Ni < Al < Cu and precious metals Ag < Pt < Au. The analysis showed among base metals Cu to be present in highest concentration i.e. 244.303 g/ Kg and gold to be present as 1106.6 mg/Kg.

Keywords: E-waste; Printed Circuit Boards; FE SEM; Inductively Coupled Plasma; EDAX.

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Green synthesis of copper nanoparticles for controlof urinary tract infections

1Manisha N. Chalse and 2Aniroodha V. Pethkar

1Department of Biotechnology, Shivchhatrapati college, Aurangabad Maharashtra(431005),India 2Department of Microbiology Gov .Institute of Science, Aurangabad, Maharashtra (431004),India.

ABSTRACT

Research on exploration of for green routes for the synthesis of copper nanoparticles is being carried out with the objectives to overcome the potential health hazards. In the present research facile, ecofriendly copper nanoparticles (CuNpls) were synthesized from copper acetate and aqueous extract of buds of Syzygium aromaticum plant . The CuNpls were characterized by UV-visible spectroscopy, showed surface plasma resonance at 750nm. Fourier transform infrared spectroscopy (FTIR) was utilized to verify about CuNpls surface state, chemical bonds and functional groups constructed in the atom groups apparent on their surface. X-ray diffraction pattern (XRD) revealed that the size of the copper nanoparticles was 23nm. The CuNpls were visualized with an atomic force microscope (AFM) which revealed spherical particles . The synthesized CuNpls were explored as a potent nanomedicine against pathogens responsible for urinary tract infections (UTI) using well diffusion method at the level of 20/mg ml. For this , UTI pathogens exhibiting multi drug resistance(MDR) were procured from clinical samples. The results clearly demonstrated anti- MDR activity of the laboratory synthesized CuNpls. The minimum inhibitory concentration( MIC) of CuNpls was found to be 50µg/ml. The present study highlights synthesis of CuNpls using completely green protocol which is simple rapid and carried out under ambient conditions.

Keywords: Copper nanoparticles, green synthesis, UTI, antibacterial multi-drug resistance.

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Studies on phytochemical characteristics of in vitro callus development of Celosia argentea L.

Yawalkar R. R. 1*, Jadhav D. S1. Kharat A. S.2 and Kulkarni J.A.3

Dept.of Biotechnology, Dr. B.A.M.U. Sub-campus Osmanabad . S. M. Dnyandeo Mohekar Mahavidyalaya, Kalamb, Dist. Osmanabad. School of Life Sciences, Jawaharlal Nehru University, New Delhi

Abstract

Plants being the most abundant on the earth contain many chemicals in them which are used for to synthesize drugs for curing diseases. These chemicals are naturally present in any parts of plant like bark, leaves, flowers, roots, fruits and seed etc. The in vitro callus developed on Murashig and skoog medium. Callus which is a undifferentiated mass of cells under the aseptic conditions. Plants bear simple and spirally arranged leaves, often pinkish or white flowers while fruits are globular and seeds are black in colour. it is also used as ornamental plant and it has medicinal value .plants different parts used in Ayurvedic medicine, phytochemical are secondary metabolites produced by all plants in which some has medicinal uses .phytochemical analysis of Celosia argentea by using various types of extract viz., methanol, chloroform, ethanol were carried out. Study reported that Celosia argentea was found to contain starch, cellulose, flavonoids, saponin, tannin, phenol, terpenoids steroids and alkaloids.

Keywords: Celosia argentea, in vitro, Phytochemical, Cellulose, Flavonoids, Saponin, Callus, Murashig and skoog medium ______

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Solanum xanthocarpum mediated silver nanoparticles synthesis and its antimicrobial potential

Rohini Pungle1, Dr. Arun S. Kharat2*

Department of Biotechnology, Shivchattrapati College, Aurangabad, MS, India School of Life Sciences, Jawaharlal Nehru University, New Delhi

ABSTRACT

Nanomaterials ranging in measurement between 1 to 100 nm have been recently reported to be of great potential for pharmaceutical, medical and cosmetic purposes. There are various physical and chemical methods to synthesis silver nanoparticles which are expensive with increased toxicity making them hazardous . Thus to have a cost effective and ergonomic method for nanoparticle synthesis, plants are look forward . Plants with secondary metabolites have great potential to synthesize nanoparticles. In present study aqueous fruit extract of Solanum xanthocarpum was explored for synthesis of silver nanoparticles (WFNP). The WFNP synthesis was optimized for temperature, pH, substrate (silver nitrate) concentration and plant extract concentration. Synthesized silver nanoparticles were characterized using UV–Visible absorption spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), zeta potential and transmission electron microscopy (TEM). UV- visible absorbance was found at 420-470. TEM analysis showed that the synthesized WFNP have average size of 20 nm. The antimicrobial activity of WFNP was explored against multidrug resistant clinical isolates viz. Escherichia coli, Shigellaspp, Aeronomonas spp, Pseudomonasspp. and Candida spp..MIC ranges in 0.16mg/ml to 2.5mg/ml at 8 McFarland’s standard. The MBC(Minimum bactericidal concentration) was found to be in between 0.312mg/ml to 5mg/ml.

Keywords:Solanum xanthocarpum, Silver nanoparticles, Antimicrobial activity, TEM, XRD

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IN VITRO CALLUS INDUCTION IN TERMINALIA ARJUNA Rushipatna Tare and Narayan B. Pandhure

Plant Tissue Culture Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431001, India.

ABSTRACT The research aims for standardization of protocol for In vitro callus induction protocol in forest and medicinal plantsTerminalia and Maduka. Terminalia Arjuna (usually simply referred to as Arjuna) is a tree bark that is used medicinally in Ayurveda for the purposes of cardiovascular health pertaining to the heart itself. It has a large variety of bioactive, with the water extract showing promise at improving left ventricle function of the heart without any observable toxicity of side effects when taken at 500mg thrice a day (every 8 hours). There are numerous human studies conducted on Arjuna bark, although many of them are low in sample size. Nevertheless, the water extract appears to be effective in improving cardiac function in persons who have recently undergone cardiac trauma or injury; Myocardial Infarction is the most commonly researched ailment in this regard. Only one study exists on otherwise healthy persons, but Arjuna showed benefit in improving left ventricle function in an exercise test and the benefits may affect a person regardless of health state. Fragile, profuse growth of callus was recorded on MS supplemented with 1 mg/lit 2,4-D. Key words: Terminalia arjuna, Callus,

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“Production of silver nanoparticle from fungal mycelium and its antibacterial activity against Pyogenic clinical isolates.”

*Aditi waghmare*, Priti Taware, Poonam Chautmal, Dipeeka Potdukhe, Priyanka Mandal, Onkar Kharat & Savita Kate*

PG Students and Asst.Prof, Shivchhatrapati College, Aurangabad

Abstract

Nanotechnology is emerging field of science which involves synthesis and development of various nanomaterials. Silver nanoparticles are undoubtedly the most widely used nonmaterial among all. In the present study silver nanoparticles was synthesize by using fourteen fungi were isolated from garden soil in PDA at 30°c for 3-4 days. For the synthesis of silver nanoparticles, 1ml of fungal mycelium extract was mixed with 10ml of 1mM silver nitrate, at 75°c. Out of 14 fungal isolates 10 shows proficient silver nanoparticle synthesis from colorless to brown colour. Characterization of synthesize AgNps was done by UV visible spectroscopy, FTIR and Zeta potential. The efficiency of synthesize AgNps as a antibacterial was demonstrated against pyogenic clinical isolates.

Keywords: - Silver Nanoparticle, Fungi, UV, FTIR, Pyogenic bacteria, Zeta potential.

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Isolation, characterization and antibiogram of copper resistant bacterial strain from industrial waste water and soil.

*1Warkhade B.B., 2Goregaonkar S.S., 3Tiwari S.R. *1Department of Biotechnology, Model College Ghansawangi.Dist.Jalna.Maharashtra, India, 431209. 2Institute of Pharmacy Badnapur, Dist. Jalna. Maharashtra, India, 431202. 3Department of Biotechnology, Bhausaheb Deshpande College of Biotechnology, Jalna, Maharashtra, India, 431203.

ABSTRACT

The pollution of the environment with heavy toxic metals is spreading all through the world along with industrial progress. Microorganism have developed several mechanisms to tolerate high concentration of heavy metals, for this purpose screening of copper resistant bacteria has been attempted in these study. Total five copper resistant bacteria were isolated from industrial waste water and soil sample. Based on morphological and biochemical characterization these isolates were identified as Bacillus spp., (S1, W1), Pseudomonas spp.,(S2) and Staphylococcus aureus. (W2, W3).The MIC of the isolates were tested with varying concentration of copper from 50 µg/ml to 1200µg/ml. The antibiogram was done, it was found copper resistant bacteria exhibited high resitant towards group of antibiotics.

Keywords: Copper resistant, Minimum inhibitory concentration, Antibiogram.

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STUDTY OF PLANT TISSUE CULTURE METHOD

S.P. Gaikwad , Head, Dept. Of Botany, Late.Nitin Arts & Science College, Pathri, Dist. Parbhani

ABSTRACT

Plant research often involves growing new plants in a controlled environment. These may be plants that we have genetically altered in some way or may be plants of which we need many copies all exactly alike. These things can be accomplished through tissue culture of small tissue pieces from the plant of interest. These small pieces may come from a single mother plant or they may be the result of genetic transformation of single plant cells which are then encouraged to grow and to ultimately develop into a whole plant. Tissue culture techniques are often used for commercial production of plants as well as for plant research.

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N-16 Optimization of fermentation conditions for L-glutaminase production by marine halotolerant Pseudomonas aeruginosa MM-2

Mohammed Mujahed1, B.M. Kareppa2, Nilesh V More3, S.H. Tarte4 1, 3 & 4 Biotechnology Research Centre, COCSIT, Latur-413531, M.S. India. 2 Head, Department of Biotechnology, D.S.M. College, Parbhani-431401, M.S. India.

Abstract

Optimization of fermentation conditions for improved production of L-glutaminase by Pseudomonas aeruginosa MM2 was carried out. Effect of different physicochemical parameters namely initial pH, temperature, Nacl concentration, L-glutamine concentration, inoculum size and supplementary carbon and nitrogen sources were studied. The isolated marine bacteria Pseudomonas aeruginosa MM2 showed the maximum production of enzyme at pH 8, temperature 35°C , 3% Nacl concentration, 1.5% L-glutamine concentration . The glucose as carbon source has supported the highest yield of L-glutaminase amongst different carbon sources. One of the major use of L-glutaminase is in the food industry as a flavor enhancing agent.

Keywords: L-glutaminase, halotolerant, inoculum size, Pseudomonas aeruginosa.

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Additional Abstracts

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1.

STUDY OF ZOOPLANKTON DIVERSITY IN PASHAN LAKE, PUNE MAHARASHTRA

Dr.A.V.Andhale1 &Dr.Gunwati V.Arak2 Aquatic Biology Lab, Department of Zoology, NowrosjeeWadia College, Pune, Maharashtra, INDIA

Abstract

The present study was carried out on a freshwater lake, located near rapidly developing Pune urban area, in the state of Maharashtra, India. The study includes physical, chemical and biological features of the lake, namely Pashan Lake. Zooplankton is the important component of aquatic flora serve as a major component of aquatic food chain. Also it maintain proper equilibrium between biotic and abiotic components of the aquatic ecosystem. The present investigation deals with study of diversity zooplankton of Pashan lake. The work was carried out for the period of one year that is December 2017 to November 2018. The zooplankton of Pashan lake water is represented by five different groups like Protozoa, Rotifera, Cladocera, Copepoda, Ostracods with 16 different species were identified and recorded in Pashan lake. Rotifera dominant among zooplankton and this indicates the polluted nature of the lake water.

Key words: Zooplankton, Pashanlake and Diversity.

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2.

Some Traditional handicrafts made from plants in the villages of Hajjah District in Yemen.

Hussein* &Dhabe, Arvind S. **

*University of Hajjah Email: [email protected] ** Department of Botany, Dr.BabasahebAmbedkarMarathwada University, Aurangabad, 431004, India.Email:[email protected]

Abstract

Yemen is famous for its many ancient heritage and traditional handicrafts, which have been common for a long time. These works still exist to this day and are based on the plants found in the environment. These traditions have been inherited and made by the generations. This study documented has so far mainly discussed some plants products in the villages of Hajjah District in Yemen. The current paper summarizes the use of some plant as general use. Plant species used by the local people to manufacture their household tools such as baskets, matshats and cords, as well as doors and windows for houses, including use in the manufacture of agricultural tools and others, the most important of these plants such as; Aervajavanica L. (Burm.f.) Juss, Raphiaruffia (Jacq.) Mart, Agavesisalana Perrine, Dracaena serrulata Baker, Cordia Africana lam, EhretiacymosaThonn, Lagenariasiceraria (Molina) Standl, GrewiamollisJuss, Bambusa vulgaris Schrad. This traditional knowledge was documented by interviews with local people, and some of the plant species used through interviews with local specialists. Key words:Traditional handicrafts, Hajjah, Yemen, Hand-manufacture, Plant diversity.

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3

Toxicity impact of Cestrum nocturnumon respiratory metabolism of Fish Clarias batrachus

Jawale C.S. and Singh-Gupta S. Department of Zoology, HPT Arts & RYK Science College, Nashik 422005, Maharashtra. (Corresponding author Dr. Jawale C.S. email: [email protected]) (Contact details: Dr. Jawale C.S. 9422770869, [email protected])

ABSTRACT

The effect of alcoholic extract of C. nocturnumwas observed on Clariasbatrachus and

LC50 value was noted. The animal was exposed to ⅓LC50 value, (0.933 ppm) for 48 hrs.

Oxygen consumption was decreased in the treated fish as compared to the control fish. Reduced SDH and elevated LDH levels were observed in liver, gills and muscles upon exposure to piscicidal extract. This indicates that the piscicidal extract of C. nocturnumleaves impairs the respiratory enzyme activity in Clariousbatrachusand hence has a lethal effect.

KEY WORDS: Cestrum nocturnum, piscicide, LDH, SDH, Clariasbatrachus, Oxygen

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4

A NEW SPECIES OF THE GENUS TYLOCEPHALUM LINTON (1890) FROM INTESTINE OF MARINE FISH EREGOODOO

Ashok mote

Dept. of Zoology, SantRamdas College Ghansawangi

------Abstract

The present communication deals with the new species of the genus tylocephalon Linton (1890) tylocephalonnandeoi. The present new speciesT.nandeoviis having the different character.Scolex large, globular, accessory suckers medium, neck short, mature segment small longer than broad testis small round 77-82 cirrus small, vasdefference thin, short, straight, ovary small bilobed, vagina wide, tubuler, ootype post ovarian, genital pore small oval, vitellaria granular.

Key words- Tylocephalum, Linton (1890), lecanicephalidae, Braun (1900).

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5 Note on Taxonomic Identity of Grewiaheterotricha Mast. from India (Malvaceae -Grewioideae). Vijay A. Paithane & amp; A.S. Bhuktar *

AnandibaiRaorane Arts, Commerce and Science College, Vaibhavwadi, Sindhudurg-4169810and *Vivekanand Arts, S.D. Commerce & Science college, Samarth Nagar, Aurangabad –431002, Maharashtra.

Abstract

Grewia heterotricha Mast. has been treated here as a taxonomic synonym of GrewiaumbelliferaBedd. is found in India. Keywords:-Grewiaheterotricha Mast., new synonym.

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6 Comparative studies of bacterial degradation of azo dye Direct Red 81

Sahasrabudhe Madhuri

Associate Professor, Dept of Microbiology, Maulana Azad College,

Aurangabad, Maharashtra, India

[email protected]

Abstract

Textile and dye industries generate large volumes of waste water. The generation and disposal of insufficiently treated of coloured wastewaters is environmentally hazardous. Such wastewater leads to dye contamination of the environment such as soil and natural water bodies. Coloured wastewater is aesthetically unacceptable and it also affects aquatic ecosystem by decreasing the light penetration and solubility of gases. Some synthetic dyes such as azo dyes are carcinogenic or mutagenic. Biological treatment methods are cheap and offer best alternative as compared to physico-chemical methods.

Microbial decolourization and degradation is environmentally friendly and cost competitive alternative to physico-chemical decomposition processes for the industrial effluents. In the present study, Direct Red 81 was used a model azo dye. Bacteria selected for the study include Enterococcus faecalis, Georgeniasp, Micrococcus glutamicusNCIM 2168, Bacillus cereus and Pseudomonas sp. Effect of various physico chemical factors was studied to detect optima for pH, temperature and dye concentration. Degradation was confirmed by Uv-Vis absorption, TLC, HPLC studies. Enzymes involved in degradation studies were found to be oxidoreductases. Toxicity studies revealed nontoxic nature of the degradation products.

Hence the isolates having ability to degrade and detoxify the dye can be successfully used for treatment of coloured wastewater.

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Screening, Isolation and Detoxifying of hexavalent Cr(VI) by Bacillus

Mhaske S.B. and Kulkarni R. A.

GovernmentCollegeofArtsandScience,Aurangabad,Maharashtra431001GovernmentCollegeofArtsandScience,A urangabad,Maharashtra431001

Abstract

Industrial pollution is generally referred to undesirable out come when factories like paint, electroplating and tannery industries allows harm fulby-product like metals and was teinto environment such as air or water bodies, deposition on land fill sector emission of toxic chemicals into atmosphere. Chronic health issues are high due to soil contaminated with industrial waste. So microorganisms can be used as apotential detoxifires of such metals. Metal containing waste can be biotechnologically treated to either recyclemetal values from the waste or to remove un wanted metal-compound. In this paper species of Bacillus is used to detoxify the hexavalent chromium from the metal containing solution. The isolates were screened by enriching the soil in the Nutrient broth. The cultures were isolated by streaking on the Nutrient agar plates. Later the isolates were exposed to 25-100 ppm concentration of Potassium dichromate solution in Nutrient broth from which 3 isolates were streaked on nutrient agar plates containg 100ppm of Potassium dichromate solution.The identified isolates were used as a potent detoxifiers and further used for chromium detoxification

Department of Botany, Deogiri College, Aurangabad. Page 304

Emerging Trans-Disciplinary Approaches in Bio-Sciences – 2019 ETDAB-2019 ______

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An Insight into E-waste: Exploring its toxicity and preciousness.

1Sonawane P.M and 2Gupta S.G. 1Department of Biotechnology, Government Institute of Science, Aurangabad (M.S), India.2 Director, Government Institute of Science, Aurangabad (M.S), India.

Abstract

According to Associated Chambers of Commerce and Industry of India (ASSOCHAM) 2 million tonnes per annum (TPA) of E-waste is generated by India of which 12% is contributed by telecom equipment alone. Apart from India, other top listed generators of E-waste are United States, China, Germany and Japan. Considering the global rise in e- waste this review is thus intended to understand the two diametric aspects of e-waste. One is its high degree of metal toxicity and the other is the valuable treasure of precious metals that it harbours. The conventional methods of dumping and burning of e-waste is deteriorating the health of our planet. The world we are living in is getting polluted by numerous toxic metals leaching out of e-waste. The sea water, ground water, land and air are getting affected everywhere. E- waste toxicity is majorly exerting negative health effect on the urban poor involved in recycling of ewaste manually. The most vulnerable been women and children. Chromium, Lead, mercury, indium, antimony, molybdenum and vanadium are one of the several toxic and allergenic metals contained in e-waste. Most of these compounds are released during recycling of e-waste. Manual recycling of e-waste by non-formal sector of society is aware of the precious metal content of e-waste but ignorant about its devasting effect on community health and environment. Gold, platinum, palladium, silver and copper are the key precious metals inside the womb of e- waste. Thus, urban mining for pulling valuable metals for economic prospects is becoming popular among most developing countries. This review paper aims to spread awareness among consumers to understand the excess obsolescence generated because of fast replacement of electronic gadgets. As a responsible citizen we must develop a positive attitude in maintaining the health of our planet by reducing, reusing and recycling e-waste. Keywords: E-waste; ASSOCHAM; Gold, Lead; Obsolescence; recycle.

Department of Botany, Deogiri College, Aurangabad. Page 305

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9

Anatomical Studies of T. rajasthanensisBhandari& Sharma of Zygophyllaceae

Pathak M P 1 and Kulkarni G B 2

1. Research Fellow, 2. Associate Professor, Department of Botany,

JES R.G. Bagdia Arts, S.B. Lakhotiya Commerce and R. Bezonji Science College,Jalna- 431203 (M.S.)

Email id: [email protected]

Abstract

A study has been carried out on member of Tribulus species in the Marathwada region of Maharashtra, in India. To find out whether the Tribulusrajasthanensis species showing anyidentical characters reported from above regions are indeed same or different in morphologicallyand anatomically. Anatomy of root, stem and leaf of T. rajasthanensis Bhandari& Sharma have been studied. The root and stem showed the presence of calcium oxalate crystals in cortex and pith region, sclerenchymatous patch on outer side of vascular bundle. Leaf showed the presence of small vascular bundles in the lamina and large curved vascular bundle in mid rib region. Leaf showed the presence of anisocytic type of stomata and unicellular, uniseriate, unbranched, conical and pointed trichomes with swollen base.

Key words:, T. rajasthanensis, anatomy, trichome, stomata, calcium oxalate crystals

Department of Botany, Deogiri College, Aurangabad. Page 306

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10

Analysis of Enzyme Activity during Vermicomposting of Organic Wastes (Corn cob and Cauliflower) employing the Earthworm Eudriluseugeniae

Vishal Bedre1 and BalrajKhobragade*

PG Department of Zoology, Ahmednagar College, Ahmednagar – 414 001 Maharashtra (India) *Corresponding author: [email protected]

1Present address: Department of Zoology, TuljaramChaturchand College of Arts, Science & Commerce, Baramati, Dist. Pune (Maharashtra) India.

ABSTRACT

The quality of vermicompost depends on the substrate acted upon by enzymes in the earthworm gut. The extent of enzyme activity varies as per the substrate and the stage of vermicomposting operation. Keeping this in view, the present study was undertaken to analyse the enzyme activity during various stages of vermicomposting of the corn cob and cauliflower waste using the earthworm species Eudriluseugeniaepopularly knownas ‘African Night Crawler’. Enzyme activity in the gut of earthworm during the composting process was investigated, which revealed that corn cob showed good enzyme activity as compared to cauliflower waste. Enzymatic analysis provided evidence of presence of the enzymes namely amylase, invertase and urease in the gut of Eudriluseugeniae. Quantitative analysis of the enzymes recorded maximum amylase and invertase activity to be 11.2 and 9.6 µg/ml/min and 8.33 and 6.46 µg/ml/min at 30th day interval; whereas, maximum ureaseactivity was found to be 3.8 and 6.1 µg/ml/min at 45th day interval; in the gut content of Eudriluseugeniae fed on corn cob and cauliflower waste, respectively. It is suggested that corn cob waste and cauliflower waste can be used as good organic substrate in vercomposting by the earthworm species Eudriluseugeniae. It can be concluded that ‘vermitechnology’ is an efficient and secure substitute tool having tremendous prospective in organic waste management.

Keywords: Vermicomposting, organic wastes, earthworm gut enzymes, Eudriluseugeniae

Department of Botany, Deogiri College, Aurangabad. Page 307 Deogiri College, Aurangabad Department of Botany National Conference 23-24 December, 2019 ETDAB 2019 Sr. Committee Members Work No. 1. Registration Dr. A. S. Taware To register the delegates on the Committee Dr. Dipti Lilhare spot counter and provide 1. Early Bird Dr. Yogesh Reddy conference kit, maintain the 2. Spot Dr. Anjum Shaikh record. Registration Mrs Nandini Watwe (Name and other details should Ms Pradnya Awate be necessary for providing the Ms. Dipali Mahajan certificate) Ms. Samiksha Pande List will be provided early bird Ms Yogita Patil registration. Ms. Swati Gaikawad 2. Stage Committee Ms. Srushti Kale Felicitation of guest Mr. Aniket Karpe (Collect the materials with Mr. Hanumant Kayande flowers and bouquet) Dr. Varsha Kanase (P.G. Volunteers) 3. Food Committee Dr. Rajesh Shesham To look after Breakfast, Tea, Dr. Kalamse Lunch and Dinner properly Dr. Nisal N.S. served) Dr. Varsha Kanase To provide water as per Ms Sadhna Salwe requirement) Ms Nilam Tupe Mrs Dipali Tribhuvan Mrs Dolly Jain 4. Inauguration Dr. Suchita Rajurkar Anchoring, Felicitation, Vote of Committee Mrs Dolly Jain thanks & instructions, keynote Ms Srushti Kale address, Introduce Chief Guest. 5. Technical Session Introduction of resource person 1. Session I Dr. Suchita Rajurkar and announcement 2. (Session II) Dr. Anilkumar Pardeshi To arrange Chairperson, Co- 3. ( Session III) Dr. Meena Nagawanshi chairman, Rapotiers with kind (Last session Dr. Shahajahan Shaikh permission of Org. Secretary. & valedictory Dr. Dipti Lilhare) *To select Top three awards amongst the presenter.

6. Poster Session 1. Dr. Yogesh Reddy To arrange the poster 2. Dr. Bharat Pandit presentation in the lawn with 3. Dr. Varsha Kanase proper numbering as per 4. Mr. Hanumant Kayande registration.(To collect required 5. Mr. Aniket Karpe material for the same) 6. Ms. Swati Gaikawad *To select Top three awards 7. (Research Scholars) amongst the presenter. 7. Certificate 1. Dr. A. B. Pardeshi To fill proper information of 2. Dr. Aparna Taware attended delegates 3. Dr. Dipti Lilhare To take signature of Convener 4. Dr. Yogesh Reddy and Organizing Secretary. 5. Mr. Bharat Pandit To maintain certificate issue 6. Mr. Aniket Karpe record. 7. Mr. Hanumant Kayande 8. Dr. Sudhir N. Solanke 8. Banner & Food 1. Dr. Kailas Thombare To prepare the banner and food coupons 2. Dr. Yogesh Reddy coupons 3. Dr. Bharat Pandit 4. Mr. Aniket Karpe 5. Dr. Sudhir N. Solanke 9. Conference Kits 1. Dr. Aparna Taware To prepare conference kit with 2. Dr. Dipti Lilhare all materials like bag containing 3. Dr. Yogesh Reddy Conference pad, pen & 4. Dr. Bharat Pandit coupons 5. (Research Scholars) To maintain record and total 6. Mr. Prakash Kedare number of kits 7. Mr. Anil Hiwarale 8. Ms. Padul mam 10. Hospitality 1. Dr. S. N. Helambe Welcome and honorarium (ResourcePersons) 2. Dr. R. P. Pawar To maintain receipt voucher 11. Publicity Dr Anilkumar Pardeshi Press conference, Pre and post Dr. Sudhir N. Solanke conference, Press release and Dr. Maruti Gaikwad news publicity. 12. Technical Mr. Girish Dudhgaokar Projector, Mic, Collar mic,Sound Mr. Shri Krishna Mhaske system, Web site, E-Souvenir Ingle and Vanarse inauguration. 13. Hotel and Stay 1. Yogesh Reddy Hotel contacts and receive the Committee 2. Aniket Karpe calls from delegates. 14. Travel and Vehicle 1.Pravin Kolhe To arrange vehicles. 15. Flower bouquet 3. Pravin Kolhe To Purchase fresh Flower and stage floral 4. Prakash Kedare Bouquet and floral garlands and decoration decorations

Organizing Secretary Convener & Principal