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Issue Full File Bazı Etanolik Propolis Örneklerinin α-Glukosidaz ve α- Α-Glucosidase and α-Amylase Inhibition of Some Ethanolic Amilaz İnhibisyonu Propolis Samples Nimet BALTAŞ Nimet BALTAŞ Güneybatı Ege Bölgesinde Nosema Türlerinin Mikroskobik Mıcroscopıc and Molecular Detectıon of Nosema Sp. in the ve Moleküler Olarak Belirlenmesi Southwest Aegean Region Serengül Kartal, Rahşan İvgin Tunca, Okan Özgül, Kemal Serengül Kartal, Rahşan İvgin Tunca, Okan Özgül, Kemal Karabağ, Hasan Koç Karabağ, Hasan Koç Akarisit Amitraz ve Fluvalinat'ın Bal Arısı Apis mellifera'nın Effect of Miticides Amitraz and Fluvalinate on Üreme ve Verimliliğine Etkisi Reproduction and Productivity of Honey Bee Apis Mellifera Rustem A. ILYASOV, Sooho LIM, Myeong Lyeol LEE, Hyung Rustem A. ILYASOV1,2*, Sooho LIM1 Myeong Lyeol LEE1, Wook KWON, Alexey G. NIKOLENKO Hyung Wook KWON1*, Alexey G. NIKOLENKO Efficacy Of Essential Oils of Thymbra capitata L. and Tunus'ta Toplanan Thymbra capitata L. ve Mentha pulegium Mentha pulegium L. Collected in Tunisia on Larvae of L. Esansiyel Yağlarının Galleria mellonella L. Üzerine Etkisi Galleria mellonella L. Sarra NCIBI, Abir BEN AMOR, Faten BEN ABDELKADER Sarra NCIBI, Abir BEN AMOR, Faten BEN ABDELKADER Tunceli Ballarının Coğrafi İşaret Çalışması Geographical Indication Study of Tunceli Honeys Aslı ÖZKÖK, Ömür GENÇAY ÇELEMLİ, Golshan ZARE, Aslı ÖZKÖK, Ömür GENÇAY ÇELEMLİ, Golshan ZARE, Çiğdem ÖZENİRLER, Nazlı MAYDA, Kadriye SORKUN Çiğdem ÖZENİRLER, Nazlı MAYDA, Kadriye SORKUN Kestane (Castanea sativa) Balı Örneklerinin Botanik Confirmation of botanical origin and total pollen numbers Orijinlerinin Doğrulanması ve Toplam Polen Sayıları of chestnut (Castanea sativa) honey samples Aslı ÖZKÖK, Nesrin ECEM BAYRAM Aslı ÖZKÖK, Nesrin ECEM BAYRAM Arıcılık Faaliyetleri Etkisi Altında Düzce Bal Arısı The Investigation of Variations in Düzce Honey Bee Popülasyonlarındaki Varyasyonların Morfometrik Yöntem ile Populations Under The Influence of Beekeeping Activities Araştırılması by Using Morphometric Method Songül BİR, Meral KEKEÇOĞLU Songül BİR, Meral KEKEÇOĞLU Sri Lanka Siddha Medicine'de Pediatrik Tedavilerde Balın The Role of Honey in Pediatric Treatments in Sri Lankan Rolü Siddha Medicine Pholtan Rajeev Sebastian RAJAMANOHARAN, Saravanan Pholtan Rajeev Sebastian RAJAMANOHARAN, Saravanan VIVEKANANDARAJAH SATHASIVAMPILLAI VIVEKANANDARAJAH SATHASIVAMPILLAI Arı sokmalarını etkileyen önemli şifalı bitkiler: Sistematik The Most Important Medicinal Plants Affecting Bee Stings: derleme çalışması A Systematic Review Study Aliasghar MANOUCHEHRI, Pegah SHAKIB, Fakher Aliasghar MANOUCHEHRI, Pegah SHAKIB, Fakher BIGLARYAN, Mohammadreza NAZER, Mohammad DARVISHI BIGLARYAN, Mohammadreza NAZER, Mohammad DARVISHI Bal Arılarında Probiyotik Bakterilerin Kullanımı Use of Probiyotic Bacteriae in Honey Bees Ayşe Ebru BORUM Ayşe Ebru BORUM Arı Poleni: Antioksidan Etkisi Bee Pollen: its Antioxidant Activity Hidayet TUTUN, Muhammet Mükerrem KAYA, Melike Sultan Hidayet TUTUN, Muhammet Mükerrem KAYA, Melike Sultan USLUER, Hatice Ahu KAHRAMAN USLUER, Hatice Ahu KAHRAMAN Bal Arısı Zehrinin Kompozisyonunu ve Üretim Miktarını Factors Affecting the Composition and Production Amount Etkileyen Faktörler of Honey Bee Venom Tuğçe ÇAPRAZLI, Meral KEKEÇOĞLU Tuğçe ÇAPRAZLI, Meral KEKEÇOĞLU Citation/Atıf: Baltaş, N. 2021. α-glucosidase and α-amylase inhibition of some ethanolic propolis samples (Bazı Etanolik Propolis Örneklerinin α-Glukosidaz ve α-Amilaz İnhibisyonu). U. Arı D./U. Bee J. 21:1-7, DOI: 10.31467/uluaricilik.877301 ARAŞTIRMA MAKALESİ / RESEARCH ARTICLE Α-GLUCOSIDASE AND α-AMYLASE INHIBITION OF SOME ETHANOLIC PROPOLIS SAMPLES Bazı Etanolik Propolis Örneklerinin α-Glukosidaz ve α-AMilaz İnhibisyonu NiMet BALTAŞ Recep Tayyip Erdogan University, Faculty of Arts and Sciences, Department of Chemistry, Rize, TURKEY, ORCID No: 0000-0003-4748-0665, E-posta: [email protected] Geliş Tarihi / Received: 09.02.20201 Kabul Tarihi / Accepted:18.03.2021 DOI: 10.31467/uluaricilik.877301 ABSTRACT Propolis is a natural product, and it is of a great interest due to the possible uses of non-synthetic supplements in improving metabolic disorders. To support this claim, the current study was designed and presented. In this study, six propolis extracts obtained froM different location of Turkey were investigated to prove the beneficial therapeutic properties such as inhibition potent against some enzyMes and levels of antioxidant. IC50 results of α-glucosidase (0.208-0.426 Mg/mL) and α-amylase (0.487-0.938 mg/ML) were found the variable range. Moreover, antioxidant results of them were given to support the inhibition degrees. According to the total phenolic (TPC) and antioxidant data, S4 was noted as the Most efficient saMple. Future studies are needed to investigate the biological effects of propolis, but the ultiMate evaluating showed that it could be a significant source thanks to its nutritional and clinical potential. Keywords: α-glucosidase, α-amylase, EnzyMe inhibition, Antioxidant, Propolis ÖZ Metabolik bozuklukların iyileştirilMesinde sentetik olMayan takviyelerin olası kullanıMı nedeniyle, doğal bir ürün olan propolis büyük ilgi görMektedir. Bu iddiayı destekleMek için Mevcut çalışMa planlandı ve ortaya kondu. Bu çalışmada, Türkiye'nin farklı bölgelerinden elde edilen altı propolis ekstratının tedavi aMacıyla kullanılabilMesi adına faydalı özelliklerini ortaya koyMak için bazı enziMlere karşı inhibisyon etkileri ve antioksidan seviyeleri araştırıldı. α-glukozidaz IC50 sonuçları 0,208-0,426 mg/mL ve α-amilaz IC50 sonuçları 0,487-0,938 Mg / mL aralığında bulundu. Ayrıca, inhibisyon etki derecelerini destekleMek için antioksidan aktivite sonuçları da verildi. ToplaM fenolik (TPC) ve antioksidan verilerine göre, S4 en veriMli örnek olarak kaydedildi. Propolisin biyolojik etkilerini araştırmak için ileride yapılacak yeni çalışmalara ihtiyaç vardır, ancak sonuç olarak, propolisin beslenMe ve klinik potansiyeli olarak dikkate değer bir kaynak olabileceğini gösterMektedir. Anahtar KeliMeler: α-glukozidaz; α-aMilaz; EnziM inhibisyonu; Antioksidan; Propolis GENİŞLETİLMİŞ ÖZET arı ürünüdür. Kovanı enfeksiyonlardan koruma amaçlı arıların ürettiği propolisin rengi reçinenin Amaç: Propolis bal arıları tarafından ağaçlardan, ve kaynağına bağlı olarak açık sarıdan koyu bitkilerin tomurcuk, yaprak, gövde ve salgılarından kahverengiye kadar değişebilir. Propolisin çok çeşitli toplanan maddelerin başlarında bulunan salgı fenolik ve flavanoid maddeler içermesi sebebiyle bezlerinden salgıladıkları enzimlerle işleyerek eski yıllardan beri geleneksel tıpta birçok hastalığın ürettiği çeşitli miktarlarda esansiyel ve aromatik tedavisinde kullanıldığı bilinmektedir. Yapılan birçok yağlar, balmumu ve reçine karışımı içeren doğal bir Uludağ Arıcılık Dergisi – Uludag Bee Journal 2021, 21 (1): 1-7 1 ARAŞTIRMA MAKALESİ / RESEARCH ARTICLE bilimsel çalışmada propolisin antibakteriyel, antiviral, Sonuç: Bu çalışmada, Türkiye’nin farklı illerinden antioksidan, antienflamatuar, antifungal, antitümör temin edilen propolis örneklerinin yüksek ve antiülser gibi birçok biyolojik aktivitelere sahip antioksidan aktiviteye sahip olduğu, α-amilazın ve α- olduğu gösterilmiştir. Bilinen birçok hastalığın glukozidazın enzimatik aktivitesini engellediği tedavisi hastalık ile ilişkili enzimlerin inhibisyonu gözlendi. Önemi her geçen gün daha da iyi yada tam olarak aktivitesinin durdurulmasıyla anlaşılmakta olan ve ender bulunan geniş mümkün olmaktadır. Bu çalışmada, propolisin sahip spektrumlu bir antibiyotik sınıfında adından söz olduğu farmakolojik özelliklerinden yola çıkarak Tip- ettiren propolisin, antidiyabetik doğal bir ürün 2 diyabet ile yakından ilişkili olan α-amilaz ve α- olabileceği söylenebilir. glukozidaz enzimleri üzerine inhibisyon etkisi ve antioksidan aktiviteleri in-vitro olarak incelendi. INTRODUCTION Gereç-YönteM: Ağustos 2019 yılında Türkiye’ nin 6 farklı ilindeki (Ankara, Kars, Giresun, Erzurum, Propolis is a resinous substance produced by Düzce, Zonguldak) Arı Yetiştiricileri Birliği’nden honeybees (Apis mellifera) from various leaf buds propolis örnekleri temin edildi. Propolis örneklerinin and plant exudates, which is used to seal and repair %70’lik etanol içerisinde ekstraktları hazırlandı. unwanted open spaces in the hive. Also, it is superior Hazırlanan propolis öneklerinin diabetes mellitus ile to other bee products because of its crucial yakından ilişkili olan, özelllikle ağız ve midede bioactivity content such as antioxidant, antimicrobial, nişastanın sindiriminden sorumlu α-amilaz enzimine anticarcinogenic, antimutagenic etc. (Baltas et al. ve bağırsaklarda disakkaritlerin sindirimini 2016, Miguel et al. 2014). gerçekleştiren α-glukozidaz enzimine karşı inhibitor Nowadays and from ancient times, people have etkisi incelendi. Her iki enzim için IC50 değerleri (ortamda var olan enzim aktivitesini yarıya düşüren used complementary therapies to protect and cope propolis konsantrasyonu) belirlendi. Pozitif kontrol with different diseases. Diabetes mellitus (DM) is a (standart ilaç) olarak akarboz kullanıldı. Ayrıca, metabolic disorder containing multiple etiologies can ekstraktaların toplam fenolik madde miktarı Folin– be characterized by chronic postprandial Ciocalteu metodu kullanılarak gallik asit eşdeğeri hyperglycemia with disturbances of carbohydrate, fat, and protein metabolism. The results of this cinsinden belirlendi. Propolis örneklerinin serbest metabolic disorder defects general imbalance radikal temizleme aktiviteleri ABTS [2,2 -azino-bis (3-etilbenzotiyazolin-6-sülfonik asit)]
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