DOCSLIB.ORG

Research Article Antidermatophytes from Bioactive Secondary Metabolites of Local Streptomyces Spp

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Research Article Antidermatophytes from Bioactive Secondary Metabolites of Local Streptomyces Spp Journal of Innovations in Pharmaceuticals and Biological Sciences JIPBS www.jipbs.com ISSN: 2349-2759 Research article Antidermatophytes from bioactive secondary metabolites of local Streptomyces spp. Ahmed A. Hamed1, Mohamed S. Abdel-Aziz1, Mohamed Fadel1*, Mohamed F. Ghali2 1Microbial Chemistry Department, National Research Centre, 33 El- Bohouth St. Dokki- Giza- Egypt- P. O. 12622. 2Botany Department, Faculty of Science, Zagazig University, Egypt. Abstract Dermatophytes were considered as pathogenic fungi that invade human skin, nails and hairs and cause dermatomycosis. One hundred and eleven were isolated from both marine and terrestrial Egyptian habitats were screened for their ability to produce bioactive secondary metabolites with antidermatophytic this has been done by cultivating them on ISP2 liquid media for 10 days. The antdermatophytic activity was tested for the produced extracts by well-diffusion methods. Extracts from isolates A1, A3, A10, G5 and P4 exhibited potent antidermatophytic activity against trichophyton mentagrophytes (RCMB 09285), Microsporumcanis (RCMB 07321) and Microsporumgypseum (RCMB 07336). These potent isolates were identified by studying their morphological, physiological and biochemical characteristics and microscopical studies. Key words: Antidermatophytes, Bioactive secondary metabolites, Streptomyces. *Corresponding Author: Mohamed Fadel, Microbial Chemistry Department, National Research Centre, 33 El- Bohouth St. Dokki- Giza- Egypt- P. O. 12622. 1. Introduction Natural products (secondary metabolites) Actinomycetes, the cultivable group of considered the most important source of Gram-positive, filamentous bacteria from potential drug leads to be used diverse ecological niches, they are in drug discovery [1], furthermore there is saprophytes and are responsible for the an urgent need to discover new antibiotic degradation of complex biopolymers [5]. to counter and reverse the spread of Actinomycetes considered as one of the antibiotic resistant pathogens [2] and to most important suppliers of antibiotics. combat life-threatening diseases [3]. Even Further, they can produce an array of though there are a considerable progress secondary metabolites, many of which within the fields of chemical synthesis and have antibacterial or antifungal engineered biosynthesis of antimicrobial properties. In fact, most antibiotics compounds, nature still remains the most developed from human pharmaceutical important source for new antibiotics [4]. use are produced by actionmycetes, and ©JIPBS, All rights reserved Mohamed Fadel et al., JIPBS, Vol 3 (2), 155-165, 2016 many being derived from Streptomyces sp. Collection of marine and soil samples [6]. Streptomyces is the most predominant Marine Samples were collected from genera representing about 57% of the different locations of the Red sea (Ghardga), total soil actinobacteria population], and Marine. Ain Sokhna Sediment and Ras Sedr is widely distributed in terrestrial and sediment. Terrestrial samples are collected aquatic habitats [7]. This member of the from Mansoura governorate, National order Actinomycetales with complex life research, Dokki, Giza NRC and Pyramids cycle involving three stages of zone, Giza soil. All the samples were kept in differentiation. It is thought that the sterile tubes in refrigerator at 4oC for morphological and physiological further analysis. differentiation and onset of secondary metabolites production result from Isolation of Streptomyces spp. common elements of regulation [8]. Streptomyces spp. were isolated by serial Dermatomycosis is a skin mycotic disease dilution technique [14] in which Sample of skin caused by Dermatophytes. were added to a sterile saline solution Pathogenic fungi, which invade the (0.85% NaCl) in a percent of 1:10(w/v or keratinized and cutaneous areas of the v/v) starch casein (SCA) of the following body (nail, hair and skin). Most of them constituents (g/l): Soluble Starch (10); belong to the Hyphomycetes but several KNO3 (2.0); Casein (0.3) MgSO4.7H2O are now known to have perfect states (0.05); FeSO4.7H2O (0.01); CaCO3 (0.02); (teleomorphs) in the family agar, 20.0 and distilled water (1000 ml) in Gynmoascaceae of the order Eurotiales case of soil isolation and filtered aged sea [9,10]. The dermatophytes are water in case of marine isolation the pH was represented by three genera: adjusted to 7.2. Proper antibacterial Microsporum, Trichophyton, and (Penicillin G) and antifungal (Nystatin) Epidermophyton on the basis of agents were added. After sterilization at morphology of their macro and 121oC for 20 min. the media were poured microconidia. The teleomorphs of the into Petri dishes and left over night to Microsporum and Trichophyton spp. are prevent moisture film [15]. 100 µl from called nannizia and arthroderma each dilution were used to inoculate a Petri respectively, however an asexual form of dish of 10 cm diameter containing about 20 Epidermophyton floccosunt is ml medium and the inoculums was spread unknown[11]. As the dermatophytes have on the dish top using a sterilized glass rod. developed resistance to antimycotic drugs The inoculated plates were incubated at [12], there is an urgent need to discover 28oC for 7-14 days and noticing any growth. safe and cost-effective antidermatophytes The isolation of streptomycetes based on drugs [13]. This study is undertaken with their special morphological characteristics the aim of isolating, cultivating and (deep sitting colonies, sporulation, screening of Streptomyces spp. Extracts characteristic color, etc.) The plates that for their ability to work as showed countable single colonies were antidermatophytic agents. The potent selected and purified by a streak plate Streptomyces isolates were further technique. identified by studying the morphological, cultural physiological as well as the Fermentation and extraction biochemical characteristic. Streptomyces spp. were cultivated on ISP2 medium (g/l): Glucose (4.0), Yeast extract 2. Materials and Methods (4.0), Malt extract (10.0). The pH was 156 Mohamed Fadel et al., JIPBS, Vol 3 (2), 155-165, 2016 adjusted to 6.8. One liter volume of Streptomyces species according to Shirling Erlenmeyer flasks each containing 200 ml of and Gottleib [18-20]. Alternatively, the ISP2 medium were inoculated with 5 ml strains were rather identified using the spore suspension. The inoculated flasks Bergey’s Manual of Determinative were incubated on a rotary shaker with 150 Bacteriology [21] and Bergey’s Manual of rpm rotation at 30ºC for 10 days. The Systematic Bacteriology [22]. Streptomyces cells were removed by centrifugation at 5000 rpm for 20 min, and 3. Results and discussion the culture supernatants were extracted using ethyl acetate Table (1), the ethyl Results acetate phase was evaporated till dryness Isolation of actinomycete One hundred and and used for further studies. eleven of actinomycetes was isolated from different marine and terrestrial habitats Antidermatophytic activity including Ghrgada marine sea, Ras Sedr The antidermatophytic activity of ethyl sediment, Ain Sokhna. Results in Table (1) acetate extracts were tested using the agar indicate Highest number of strains were well diffusion method [16], 100 µl of each isolated from Hurgada sea water (20 %) extract culture was placed in a well made followed by Ras sedr sediments (16 %) and with a sterile cork borer on Sabouraud soil isolates from Mansoura (17 %). dextrose agar plates (pH 5.6) seeded with the test fungal cultures Trichophyton Table 1. Distribution and percent of mentagrophytes (RCMB 09285), streptomyces spp. isolated from different Microsporum canis (RCMB 07321) and marine and terrestrial localities. Microsporum gypseum (RCMB 07336). The Isolates Percentage plates were incubated at 28oC and observed Location account incidence (%) for antibiosis after 3-4 days [17] Results are Marine sea water shown in Table (2). Amphotericin B was 22 20 % Hurgada used as a control. Twenty-one isolates were Marine algae 13 12 % primarily selected as they exhibited Ras Sedr 18 16 % interesting antidermatophytic activities. sediments Ten isolates (A1, A3, A8, A10, A12, G5, P2, Ain Sokhna 13 12 % P4, P7, P9) exhibited antidermatophytic sediment activity by a clear zone of inhibition against Marine algae 2 5 4 % all the test dermatophytes. While nine Soil from 19 17 % isolates (B13, B17, F5, F7, F12, F20, S1, Mansoura Soil from NRC S5, S14) exhibited antidermatophytic 10 9 % activity by a clear zone of inhibition against garden Microsporumcanis (RCMB 07321) and Pyramids soil 11 10 % Microsporumgypseum (RCMB 07336) and Total isolates 111 100 % two isolates(H3, H10) exhibited antidermatophytic activity by a clear zone Antidermatophytic activity test: Result in of inhibition against (Trichophyton Table 2 revealed that twenty-one isolates mentagrophytes (RCMB 09285) and exhibited antidermatophytic activities. Ten Microsporumcanis (RCMB 07321). extracts from. (A1, A3, A8, A10, A12, G5, P2, Taxonomic identification: The diagnostic P4, P7, P9) showed antidermatophytic properties of the strain were compared activity against all test dermatophytes: with those reported for identification of Trichophyton mentagrophytes (RCMB 157 Mohamed Fadel et al., JIPBS, Vol 3 (2), 155-165, 2016 09285), Microsporum canis (RCMB 07321) antifungal activity against V. ceratosperma and Microsporum gypseum (RCMB [29]. Taxonomic identification of the most 07336).While the most potent streptomyces potent actinomycetes strain (P4) has been spp. extracts were (A1, A3, A10, G5 and restricted
Load more

Recommended publications
  • Streptomyces Sannurensis Sp. Nov., a New Alkaliphilic Member of the Genus Streptomyces Isolated from Wadi Sannur in Egypt
    African Journal of Microbiology Research Vol. 5(11), pp. 1329-1334, 4 June, 2011 Available online http://www.academicjournals.org/ajmr DOI: 10.5897/AJMR11.200 ISSN 1996-0808 ©2011 Academic Journals Full Length Research Paper Streptomyces sannurensis sp. nov., a new alkaliphilic member of the genus Streptomyces isolated from Wadi Sannur in Egypt Wael N. Hozzein1,2*, Mohammed I. A. Ali3, Ola Hammouda2, Ahmed S. Mousa2 and Michael Goodfellow4 1Chair of Advanced Proteomics and Cytomics Research, Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia. 2Botany Department, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt. 3Botany Department, Faculty of Science, Cairo University, Giza, Egypt. 4School of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK. Accepted 19 April, 2011 The taxonomic position of an actinomycete isolated from a soil sample collected from Wadi Sannur in Egypt was established using a polyphasic approach. The isolate, which was designated WS 51T, was shown to have chemical and morphological properties typical of streptomycetes. An almost complete 16S rDNAgene sequence of the strain was generated and compared with corresponding sequences of representative streptomycetes. The resultant data confirmed the classification of the strain in the genus Streptomyces but also showed that it formed a distinct phyletic line within the 16S rDNAStreptomyces gene tree. The organism was most closely associated to the type strains of Streptomyces hygroscopicus, Streptomyces malaysiensis and Streptomyces yatensis but was readily separated from them using a range of phenotypic properties. It is proposed that strain WS 51T (= CCTCC 001032T = DSM 41834T) be classified in the genus Streptomyces as Streptomyces sannurensis sp.
    [Show full text]
  • Improved Taxonomy of the Genus Streptomyces
    UNIVERSITEIT GENT Faculteit Wetenschappen Vakgroep Biochemie, Fysiologie & Microbiologie Laboratorium voor Microbiologie Improved taxonomy of the genus Streptomyces Benjamin LANOOT Scriptie voorgelegd tot het behalen van de graad van Doctor in de Wetenschappen (Biochemie) Promotor: Prof. Dr. ir. J. Swings Co-promotor: Dr. M. Vancanneyt Academiejaar 2004-2005 FACULTY OF SCIENCES ____________________________________________________________ DEPARTMENT OF BIOCHEMISTRY, PHYSIOLOGY AND MICROBIOLOGY UNIVERSITEIT LABORATORY OF MICROBIOLOGY GENT IMPROVED TAXONOMY OF THE GENUS STREPTOMYCES DISSERTATION Submitted in fulfilment of the requirements for the degree of Doctor (Ph D) in Sciences, Biochemistry December 2004 Benjamin LANOOT Promotor: Prof. Dr. ir. J. SWINGS Co-promotor: Dr. M. VANCANNEYT 1: Aerial mycelium of a Streptomyces sp. © Michel Cavatta, Academy de Lyon, France 1 2 2: Streptomyces coelicolor colonies © John Innes Centre 3: Blue haloes surrounding Streptomyces coelicolor colonies are secreted 3 4 actinorhodin (an antibiotic) © John Innes Centre 4: Antibiotic droplet secreted by Streptomyces coelicolor © John Innes Centre PhD thesis, Faculty of Sciences, Ghent University, Ghent, Belgium. Publicly defended in Ghent, December 9th, 2004. Examination Commission PROF. DR. J. VAN BEEUMEN (ACTING CHAIRMAN) Faculty of Sciences, University of Ghent PROF. DR. IR. J. SWINGS (PROMOTOR) Faculty of Sciences, University of Ghent DR. M. VANCANNEYT (CO-PROMOTOR) Faculty of Sciences, University of Ghent PROF. DR. M. GOODFELLOW Department of Agricultural & Environmental Science University of Newcastle, UK PROF. Z. LIU Institute of Microbiology Chinese Academy of Sciences, Beijing, P.R. China DR. D. LABEDA United States Department of Agriculture National Center for Agricultural Utilization Research Peoria, IL, USA PROF. DR. R.M. KROPPENSTEDT Deutsche Sammlung von Mikroorganismen & Zellkulturen (DSMZ) Braunschweig, Germany DR.
    [Show full text]
  • Production of an Antibiotic-Like Activity by Streptomyces Sp. COUK1 Under Different Growth Conditions Olaitan G
    East Tennessee State University Digital Commons @ East Tennessee State University Electronic Theses and Dissertations Student Works 8-2014 Production of an Antibiotic-like Activity by Streptomyces sp. COUK1 under Different Growth Conditions Olaitan G. Akintunde East Tennessee State University Follow this and additional works at: https://dc.etsu.edu/etd Part of the Biology Commons Recommended Citation Akintunde, Olaitan G., "Production of an Antibiotic-like Activity by Streptomyces sp. COUK1 under Different Growth Conditions" (2014). Electronic Theses and Dissertations. Paper 2412. https://dc.etsu.edu/etd/2412 This Thesis - Open Access is brought to you for free and open access by the Student Works at Digital Commons @ East Tennessee State University. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of Digital Commons @ East Tennessee State University. For more information, please contact digilib@etsu.edu. Production of an Antibiotic-like Activity by Streptomyces sp. COUK1 under Different Growth Conditions A thesis presented to the faculty of the Department of Health Sciences East Tennessee State University In partial fulfillment of the requirements for the degree Master of Science in Biology by Olaitan G. Akintunde August 2014 Dr. Bert Lampson Dr. Eric Mustain Dr. Foster Levy Keywords: Streptomyces, antibiotics, natural products, bioactive compounds ABSTRACT Production of an Antibiotic-like Activity by Streptomyces sp. COUK1 under Different Growth Conditions by Olaitan Akintunde Streptomyces are known to produce a large variety of antibiotics and other bioactive compounds with remarkable industrial importance. Streptomyces sp. COUK1 was found as a contaminant on a plate in which Rhodococcus erythropolis was used as a test strain in a disk diffusion assay and produced a zone of inhibition against the cultured R.
    [Show full text]
  • Thèse Présentée Par : KITOUNI Mahmoud En Vue De L'obtention Du Diplôme De : DOCTORAT D'etat En : MICROBIOLOGIE APPLIQUEE
    République Algérienne Démocratique et Populaire Ministère de l’Enseignement Supérieur et de la Recherche Scientifique Université Mentouri-Constantine Faculté des Sciences de la Nature et de la Vie Département des Sciences de la Nature et de la Vie N ° d’ordreN° : 84 IT.E / 2007 SERIE : 05 ISN / 2007 Thèse présentée par : KITOUNI Mahmoud En vue de l’obtention du Diplôme de : DOCTORAT D’ETAT en : MICROBIOLOGIE APPLIQUEE Intitulée : Isolement de bactéries actinomycétales productrices d’antibiotiques à partir d’écosystèmes extrêmes. Identification moléculaire des souches actives et caractérisation préliminapréliminaireire des substances élaborées Membres du jury : Mr BENGUEDOUAR A. Professeur Président Univ. Mentouri-Constantine Mr BOULAHROUF A. Professeur Directeur de thèse Univ. Mentouri-Constantine Mr BOIRON P. Professeur Examinateur Univ. Lyon 1 Mr KARAM N. Professeur Examinateur Univ. Essania-Oran Mr BELLAHCENE M. Maître de Examinateur Univ. Mostaganem Conférences Mr HADDI M.L. Maître de Examinateur Univ. Mentouri-Constantine Conférences Session 2007 REMERCIEMENTS Ce travail a été réalisé au laboratoire de génie microbiologique et applications de l’Université Mentouri de Constantine. Que Monsieur Abderrahmane Boulahrouf (Professeur à l’Université Mentouri de Constantine) trouve ici l’expression de ma très vive reconnaissance pour avoir accepter la responsabilité de ce travail. Je remercie Monsieur Amar Benguedouar (Professeur à l’Université Mentouri de Constantine) de m’avoir fait l’honneur de présider mon jury de thèse. Mes remerciements vont également à Monsieur Patrick Boiron (Professeur à l’Université Claude Bernard Lyon 1) pour la confiance et l’accueil chaleureux qui ma réservé à Lyon, ses précieux conseils et d’avoir accepté de se déplacer à Constantine pour participer à ce jury.
    [Show full text]
  • Review Article
    International Journal of Systematic and Evolutionary Microbiology (2001), 51, 797–814 Printed in Great Britain The taxonomy of Streptomyces and related REVIEW genera ARTICLE 1 Natural Products Drug Annaliesa S. Anderson1 and Elizabeth M. H. Wellington2 Discovery Microbiology, Merck Research Laboratories, PO Box 2000, RY80Y-300, Rahway, Author for correspondence: Annaliesa Anderson. Tel: j1 732 594 4238. Fax: j1 732 594 1300. NJ 07065, USA e-mail: liesaIanderson!merck.com 2 Department of Biological Sciences, University of The streptomycetes, producers of more than half of the 10000 documented Warwick, Coventry bioactive compounds, have offered over 50 years of interest to industry and CV4 7AL, UK academia. Despite this, their taxonomy remains somewhat confused and the definition of species is unresolved due to the variety of morphological, cultural, physiological and biochemical characteristics that are observed at both the inter- and the intraspecies level. This review addresses the current status of streptomycete taxonomy, highlighting the value of a polyphasic approach that utilizes genotypic and phenotypic traits for the delimitation of species within the genus. Keywords: streptomycete taxonomy, phylogeny, numerical taxonomy, fingerprinting, bacterial systematics Introduction trait of producing whorls were the only detectable differences between the two genera. Witt & Stacke- The genus Streptomyces was proposed by Waksman & brandt (1990) concluded from 16S and 23S rRNA Henrici (1943) and classified in the family Strepto- comparisons that the genus Streptoverticillium should mycetaceae on the basis of morphology and subse- be regarded as a synonym of Streptomyces. quently cell wall chemotype. The development of Kitasatosporia was also included in the genus Strepto- numerical taxonomic systems, which utilized pheno- myces, despite having differences in cell wall com- typic traits helped to resolve the intergeneric relation- position, on the basis of 16S rRNA similarities ships within the family Streptomycetaceae and resulted (Wellington et al., 1992).
    [Show full text]
  • Computer Assisted Classification and Identification of Actinomycetes
    Computer Assisted Classification and Identification of Actinomycetes Jongsik Chun (B.Sc. Microbiology, Seoul National University, Seoul, Korea) NEWCASTLE UNIVERSITY LIERARY 094 52496 3 Thesis submitted in accordance with the requirements of the University of Newcastle upon Tyne for the Degree of Doctor of Philosophy Department of Microbiology The Medical School Newcastle upon Tyne England-UK July 1995 ABSTRACT Three computer software packages were written in the C++ language for the analysis of numerical phenetic, 16S rRNA sequence and pyrolysis mass spectrometric data. The X program, which provides routines for editing binary data, for calculating test error, for estimating cluster overlap and for selecting diagnostic and selective tests, was evaluated using phenotypic data held on streptomycetes. The AL16S program has routines for editing 16S rRNA sequences, for determining secondary structure, for finding signature nucleotides and for comparative sequence analysis; it was used to analyse 16S rRNA sequences of mycolic acid-containing actinomycetes. The ANN program was used to generate backpropagation-artificial neural networks using pyrolysis mass spectra as input data. Almost complete 1 6S rDNA sequences of the type strains of all of the validly described species of the genera Nocardia and Tsukamurel!a were determined following isolation and cloning of the amplified genes. The resultant nucleotide sequences were aligned with those of representatives of the genera Corynebacterium, Gordona, Mycobacterium, Rhodococcus and Turicella and phylogenetic trees inferred by using the neighbor-joining, least squares, maximum likelihood and maximum parsimony methods. The mycolic acid-containing actinomycetes formed a monophyletic line within the evolutionary radiation encompassing actinomycetes. The "mycolic acid" lineage was divided into two clades which were equated with the families Coiynebacteriaceae and Mycobacteriaceae.
    [Show full text]
  • Dactylosporangium and Some Other Filamentous Actinomycetes
    Biosystematics of the Genus Dactylosporangium and Some Other Filamentous Actinomycetes Byung-Yong Kim (BSc., MSc. Agricultural Chemistry, Korea University, Korea) Thesis submitted in accordance with the requirements of the Newcastle University for the Degree of Doctor of Philosophy November 2010 School of Biology, Faculty of Science, Agriculture and Engineering, Newcastle University, Newcastle upon Tyne, United Kingdom Dedicated to my mother who devoted her life to our family, and to my brother who led me into science "Whenever I found out anything remarkable, I have thought it my duty to put down my discovery on paper, so that all ingenious people might be informed thereof." - Antonie van Leeuwenhoek, Letter of June 12, 1716 “Freedom of thought is best promoted by the gradual illumination of men’s minds, which follows from the advance of science.”- Charles Darwin, Letter of October 13, 1880 ii Abstract This study tested the hypothesis that a relationship exists between taxonomic diversity and antibiotic resistance patterns of filamentous actinomycetes. To this end, 200 filamentous actinomycetes were selectively isolated from a hay meadow soil and assigned to groups based on pigments formed on oatmeal and peptone-yeast extract-iron agars. Forty-four representatives of the colour-groups were assigned to the genera Dactylosporangium, Micromonospora and Streptomyces based on complete 16S rRNA gene sequence analyses. In general, the position of these isolates in the phylogenetic trees correlated with corresponding antibiotic resistance patterns. A significant correlation was found between phylogenetic trees based on 16S rRNA gene and vanHAX gene cluster sequences of nine vancomycin-resistant Streptomyces isolates. These findings provide tangible evidence that antibiotic resistance patterns of filamentous actinomycetes contain information which can be used to design novel media for the selective isolation of rare and uncommon, commercially significant actinomycetes, such as those belonging to the genus Dactylosporangium, a member of the family Micromonosporaceae.
    [Show full text]
  • Taxonomic Characterization of Streptomyces Strain CH54-4 Isolated from Mangrove Sediment
    Ann Microbiol (2010) 60:299–305 DOI 10.1007/s13213-010-0041-4 ORIGINAL ARTICLE Taxonomic characterization of Streptomyces strain CH54-4 isolated from mangrove sediment Rattanaporn Srivibool & Kanpicha Jaidee & Morakot Sukchotiratana & Shinji Tokuyama & Wasu Pathom-aree Received: 19 January 2010 /Accepted: 9 March 2010 /Published online: 15 April 2010 # Springer-Verlag and the University of Milan 2010 Abstract An actinobacterium, designated as strain CH54-4, wall chemotype I with no characteristic sugar, and type II was isolated from mangrove sediment on the east coast of the polar lipids that typically contain diphosphatidyl glycerol, Gulf of Thailand using starch casein agar. This isolate was phosphatidylinositol, phosphatidylethanolamine, and phos- found to contain chemical markers typical of members of the phatidylinositol mannoside. Members of the genus Strepto- genus Streptomyces: This strain possessed a broad spectrum myces are widely distributed in soils and played important of antimicrobial activity against Gram-positive, Gram- role in soil ecology (Goodfellow and Williams 1983). They negative bacteria and fungi. In addition, this strain also are prolific sources of secondary metabolites, notably showed strong activity against breast cancer cells with an antibiotics (Lazzarini et al. 2000). −1 IC50 value of 2.91 µg ml . Phylogenetic analysis of a 16S The search and discovery of novel microbes for new rRNA gene sequence showed that strain CH54-4 forms a secondary metabolites is significant in the fight against distinct clade within the Streptomyces 16S rRNA gene tree antibiotic resistant pathogens (Bernan et al. 2004) and and closely related to Streptomyces thermocarboxydus. emerging diseases (Taylor et al. 2001). One strategy is to isolate novel actinomycetes from poorly studied habitats to Keywords Mangrove sediment .
    [Show full text]
  • Phylogenetic Study of the Species Within the Family Streptomycetaceae
    Antonie van Leeuwenhoek DOI 10.1007/s10482-011-9656-0 ORIGINAL PAPER Phylogenetic study of the species within the family Streptomycetaceae D. P. Labeda • M. Goodfellow • R. Brown • A. C. Ward • B. Lanoot • M. Vanncanneyt • J. Swings • S.-B. Kim • Z. Liu • J. Chun • T. Tamura • A. Oguchi • T. Kikuchi • H. Kikuchi • T. Nishii • K. Tsuji • Y. Yamaguchi • A. Tase • M. Takahashi • T. Sakane • K. I. Suzuki • K. Hatano Received: 7 September 2011 / Accepted: 7 October 2011 Ó Springer Science+Business Media B.V. (outside the USA) 2011 Abstract Species of the genus Streptomyces, which any other microbial genus, resulting from academic constitute the vast majority of taxa within the family and industrial activities. The methods used for char- Streptomycetaceae, are a predominant component of acterization have evolved through several phases over the microbial population in soils throughout the world the years from those based largely on morphological and have been the subject of extensive isolation and observations, to subsequent classifications based on screening efforts over the years because they are a numerical taxonomic analyses of standardized sets of major source of commercially and medically impor- phenotypic characters and, most recently, to the use of tant secondary metabolites. Taxonomic characteriza- molecular phylogenetic analyses of gene sequences. tion of Streptomyces strains has been a challenge due The present phylogenetic study examines almost all to the large number of described species, greater than described species (615 taxa) within the family Strep- tomycetaceae based on 16S rRNA gene sequences Electronic supplementary material The online version and illustrates the species diversity within this family, of this article (doi:10.1007/s10482-011-9656-0) contains which is observed to contain 130 statistically supplementary material, which is available to authorized users.
    [Show full text]
  • Insights Into the Variation in Bioactivities of Closely Related Streptomyces Strains from Marine Sediments of the Visayan Sea Against ESKAPE and Ovarian Cancer
    marine drugs Article Insights into the Variation in Bioactivities of Closely Related Streptomyces Strains from Marine Sediments of the Visayan Sea against ESKAPE and Ovarian Cancer Edna M. Sabido 1 , Chuckcris P. Tenebro 2 , Dana Joanne Von L. Trono 2 , Carmela Vannette B. Vicera 2 , Sheeny Fane L. Leonida 1, Jose Jeffrey Wayne B. Maybay 1 , Rikka Reyes-Salarda 2,3 , Diana S. Amago 1 , Angelica Marie V. Aguadera 1 , May C. Octaviano 1, Jonel P. Saludes 1,4,5,6,* and Doralyn S. Dalisay 2,3,5,6,* 1 Center for Natural Drug Discovery and Development (CND3), University of San Agustin, Iloilo City 5000, Philippines; ednasabido@usa.edu.ph (E.M.S.); sfleonida@usa.edu.ph (S.F.L.L.); jjmaybay@usa.edu.ph (J.J.W.B.M.); damago@usa.edu.ph (D.S.A.); aaguadera@usa.edu.ph (A.M.V.A.); moctaviano@usa.edu.ph (M.C.O.) 2 Center for Chemical Biology and Biotechnology (C2B2), University of San Agustin, Iloilo City 5000, Philippines; ctenebro@usa.edu.ph (C.P.T.); djvtrono@usa.edu.ph (D.J.V.L.T.); cvicera@usa.edu.ph (C.V.B.V.); rikkareyes@usa.edu.ph (R.R.-S.) 3 Department of Biology, College of Liberal Arts, Sciences, and Education, University of San Agustin, Iloilo City 5000, Philippines 4 Department of Chemistry, College of Liberal Arts, Sciences, and Education, University of San Agustin, Citation: Sabido, E.M.; Tenebro, C.P.; Iloilo City 5000, Philippines 5 Trono, D.J.V.L.; Vicera, C.V.B.; Tuklas Lunas Development Center, University of San Agustin, Iloilo City 5000, Philippines 6 Balik Scientist Program, Department of Science and Technology, Philippine Council for Health Research and Leonida, S.F.L.; Maybay, J.J.W.B.; Development (PCHRD), Bicutan, Taguig City 1631, Philippines Reyes-Salarda, R.; Amago, D.S.; * Correspondence: jsaludes@usa.edu.ph (J.P.S.); ddalisay@usa.edu.ph (D.S.D.); Aguadera, A.M.V.; Tel.: +63-33-503-6887 (J.P.S.); +63-33-501-0350 (D.S.D.) Octaviano, M.C.; et al.
    [Show full text]
  • Biological Activity of Streptomycetes Isolates from Soils of R. Moldova
    Athens Journal of Sciences- Volume 4, Issue 3 – Pages 213-224 Biological Activity of Streptomycetes Isolates from Soils of R. Moldova By Svetlana Burtseva Maxim Byrsa† Iulia Bereziuk‡ Nicola Sasanelli Actinomycetes, including the largest group Streptomyces genus, have practical importance as producers of biologically active substances used in medicine, veterinary, plant breeding and plant protection (antibiotics, vitamins, enzymes, lipids, amino acids, etc.). In the recent years, the National Collection of Nonpathogenic Microorganisms of Academy of Sciences of R. Moldova was enriched with new strains of streptomycetes, isolated from soils of different regions of R. Moldova, which were studied for their morphological and cultural characteristics, ability to synthesize exametabolites that stimulate plant growth and antagonistic activities against plant pests and pathogens. From cernoziom soils from the central part of R. Moldova 236 strains of streptomycetes were isolated and some of them were able to improve seed germination or other morphological or productive characteristics of tomato, tobacco, sugar beet and soybean plants and to completely inhibit growth of phytopathogenic fungi such as Alternaria alternata, A. niger, Botrytis cinerea and Fusarium spp. (9, 10, 12, 14, 44, 66, 185, 190, 196 and 198) and the root-knot nematode Meloidogyne incognita (9, 66 and 205). Therefore, on the base of our findings, bio-pesticide and bio-stimulators could be prepared by metabolites of these moldovian new streptomycetes strains. Keywords: Biocidal activity, Meloidogyne incognita, Phytopathogenic fungi, Streptomyces. Introduction Streptomycetes spp. are Gram-positive filamentous actinobacteria widespread in nature: in the air, in water, on plants and animal remains and especially into the soil environment where they are well adapted.
    [Show full text]
  • Molecular Studies on Some Soil-Streptomyces Strains of Western Region, Kingdom of Saudi Arabia (KSA)
    Vol. 12(19), pp. 2558-2564, 8 May, 2013 DOI: 10.5897/AJB2013.11945 African Journal of Biotechnology ISSN 1684-5315 ©2013 Academic Journals http://www.academicjournals.org/AJB Full Length Research Paper Molecular studies on some soil-Streptomyces strains of western region, Kingdom of Saudi Arabia (KSA) Mohamed, S. H.1,2, Shori Ghadeer, B. O.1 and Sadik, A. S.1,3* 1Department of Biology, Faculty of Science, Taif University, P.O. Box 888, Taif, Kingdom of Saudi Arabia. 2Soil, Water and Environmental Research Institute, Agricultural Research Center, 9 Gamaa st., P.O. Box 12619, Giza, Egypt. 3Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, P.O. Box 68 Hadayek Shubra, 11241, Cairo, Egypt. Accepted 3 May, 2013 Random amplified polymorphic of DNA-polymerase chain reaction (RAPD-PCR) analysis of the DNA extracted from seven Streptomyces strains of western region, KSA was the aim of this study. Partial sequence of 16S rRNA gene of Streptomyces polychromogenes was also attempted. Results show that a total number of 240 amplified fragments were amplified using 10 RAPD-PCR primers (OPA11, OPB10, OPB15, OPC03, OPC07, OPC18, OPD05, OPE05, OPO14 and OPO17). A total number of 97, 73, 88, 79, 100, 108 and 82 fragments were amplified from the DNA extracts of S. polychromogenes, Streptomyces chattanoogensis, Streptomyces lucensis, Streptomyces antibioticus, Streptomyces violans, Streptomyces griseorubiginosus and Streptomyces violaceus, respectively. An obvious variation in the amplified fragments was recorded using the 10 RAPD-PCR primers (31, 27, 17, 19, 23, 29, 24, 26, 23 and 21 fragments for the primers, respectively). The highest similarity (66.7%) was found between S.
    [Show full text]