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SDHAF1 Antibody Cat SDHAF1 Antibody Cat. No.: 6879 SDHAF1 Antibody Immunocytochemistry of SDHAF1 in 3T3 cells with Immunofluorescence of SDHAF1 in 3T3 cells with SDHAF1 SDHAF1 antibody at 5 μg/mL. antibody at 20 μg/mL. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse SDHAF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SDHAF1. IMMUNOGEN: The immunogen is located within the last 50 amino acids of SDHAF1. TESTED APPLICATIONS: ELISA, ICC, IF, WB October 2, 2021 1 https://www.prosci-inc.com/sdhaf1-antibody-6879.html SDHAF1 antibody can be used for detection of SDHAF1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL. APPLICATIONS: Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. SDHAF1 antibody is predicted to not cross-react with other SDHAF protein family SPECIFICITY: members. POSITIVE CONTROL: 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate 2) Cat. No. 17-201 - 3T3/BALB Cell Slide Properties PURIFICATION: SDHAF1 Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: SDHAF1 Antibody is supplied in PBS containing 0.02% sodium azide. CONCENTRATION: 1 mg/mL SDHAF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: SDHAF1 SDHAF1 Antibody: LYRM8, LYRM8, Succinate dehydrogenase assembly factor 1, ALTERNATE NAMES: mitochondrial, LYR motif-containing protein 8, SDH assembly factor 1 ACCESSION NO.: NP_001036096 PROTEIN GI NO.: 111038124 GENE ID: 644096 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References October 2, 2021 2 https://www.prosci-inc.com/sdhaf1-antibody-6879.html SDHAF1 Antibody: SDHAF1 (Succinate dehydrogenase complex assembly factor 1) is one of the subunits of the succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain. SDHAF1 resides in the mitochondria, and is essential for BACKGROUND: SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). Unlike the related protein SDHAF2, SDHAF1 is not thought to be a tumor suppressor. 1) Ghezzi D, Goffrini P, Uziel G, et al. SDHAF1, encoding a LYR complex-II specific assembly REFERENCES: factor, is mutated in SDH-defective infantile leukoencephalopathy. Nat. Genet. 2009; 41:654-6. 2) Hensen EE and Bayley JP. Recent advances in the genetics of SDH-related paraganglioma and pheochromocytoma. Fam. Cancer 2011; 10:355-63. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. October 2, 2021 3 https://www.prosci-inc.com/sdhaf1-antibody-6879.html.
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