Lab.2. Thin Layer Chromatography
Lab.2. Thin layer chromatography Key words: Separation techniques, compounds and their physicochemical properties (molecular volume/size, polarity, molecular interactions), mobile phase, stationary phase, liquid chromatography, thin layer chromatography, column chromatography, retardation factor, elution, chromatogram development, qualitative and quantitative analysis with chromatography techniques, eluotropic series, elution strength. Literature: D.A. Skoog, F.J. Holler, T.A. Nieman: Principles of Instrumental Analysis; 637 - 718 Search on www pages “Thin-layer chromatography principles” For example: MIT Digital Lab Techniques Manual you find on http://www.youtube.com/watch?v=e99nsCAsJrw&feature=player_detailpage Basic equipment for modern thin layer chromatography: www.camag.com/downloads/free/brochures/CAMAG-basic-equipment-08.pdf other examples: en.wikipedia.org/wiki/Thin_layer_chromatography www.chemguide.co.uk/analysis/chromatography/thinlayer.html www.wellesley.edu/Chemistry/chem211lab/Orgo_Lab_Manual/Appendix/Techniques/TLC/th in_layer_chrom.html Theoretical background Chromatography is the separation technique in which separated solutes are distributed between two phases: stationary and mobile. The first phase can pose a layer of sorbent/adsorbent (0.1 to 0.25 mm in thickness) fixed to a carrier plate made of glass, plastic or aluminum (used in technique named as thin-layer chromatography, TLC) or placed inside of a steel tube as a column bed (used in a technique named as high-performance liquid chromatography, HPLC, or generally in column liquid chromatography, LC). The second phase, mentioned above, constitute liquid or gas phase. Various organic (e.g. methanol, hexane, acetone) and inorganic (e.g. water) solvents or their mixtures (e.g. acetone and Lab.2. Thin layer chromatography hexane, methanol and water) can be used as the mobile phases.
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