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Bijvoet Center Progress Report 2014-2015

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Bijvoet Center Progress Report 2014-2015 Bijvoet Center for Biomolecular Research Progress Report 2014-2015 “Discovering the Molecular Basis of Life” Table of Contents Contact Details Bijvoet Center Information 2UJDQL]DWLRQ 6FLHQWLILF2XWSXW *UDQWVDQG$ZDUGV %LMYRHW6FKRRO Principal Investigators $QQD$NKPDQRYD 0DDUWHQ$OWHODDU 0DUF%DOGXV &HOLD%HUNHUV 5ROI %RHOHQV $OH[DQGUH%RQYLQ *HHUW-DQ%RRQV ,QHNH%UDDNPDQ (HIMDQ%UHXNLQN *HUW)RONHUV )ULHGULFK)|UVWHU :LOOLH*HHUWV 3LHW*URV $OEHUW+HFN &DVSHU+RRJHQUDDG (ULF+XL]LQJD %HUW-DQVVHQ $QWRLQHWWH.LOOLDQ 7RRQGH.URRQ /RHV.URRQ%DWHQEXUJ 6LPRQH/HPHHU 0DUWLQ/XW] 1DWKDQLHO0DUWLQ 0DGHORQ0DXULFH :DOO\0OOHU 5RODQG3LHWHUV +ROJHU5HKPDQQ 6WHIDQ5GLJHU 5LFKDUG6FKHOWHPD 0DUF7LPPHUV 0DUNXV:HLQJDUWK Introduction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´GLVFRYHULQJ WKH 0R KROGVLJQLILFDQWSRWHQWLDOIRUGHYHORS 5REGH9ULHVRI WKH0HGLFLQDO&KHP OHFXODU%DVLVRI /LIHµ,QWKHODVWWZR PHQWRI IXWXUHFDQFHUWUHDWPHQWV LVWU\JURXSDQG/L;XHRI WKH105 \HDUV ZH KDYH VHHQ WKH UHVHDUFK LQ VSHFWURVFRS\ JURXS $OVR IURP WKH WKHFHQWHUKDVEHHQPRYLQJWRZDUGV 6HYHUDOPHPEHUVRI WKH%LMYRHW&HQW 105 JURXS 0DUNXV :HLQJDUWK UH XQGHUVWDQGLQJ PROHFXODU DVSHFWV RI HU DOVR UHFHLYHG SUHVWLJLRXV DZDUGV FHLYHGLQD9LGLJUDQW$Q1:2 OLIH DW LQFUHDVLQJ OHYHOV RI FRPSOH[ DQGJUDQWVLQWKHSDVWWZR\HDUV,Q 9LFL JUDQW ZDV DZDUGHG WR 0DGHORQ LW\DOOWKHZD\WRZDUGVLQWDFWOLYLQJ WKH 5R\DO 1HWKHUODQGV $FDGH 0DXULFHDQGDQ(5&6WDUWLQJ*UDQW FHOOV%\H[SDQGLQJLQWRQHZUHVHDUFK P\RI $UWVDQG6FLHQFHVDZDUGHG&H ZDVDZDUGHGWR%HUW-DQVVHQ7KH(X GLUHFWLRQVZHKDYHEHHQDEOHWRFRQ OLD%HUNHUVZLWKWKH+HLQHNHQ<RXQJ URSHDQ8QLRQDZDUGHGDODUJHJUDQW VROLGDWH RXU OHDGLQJ SRVLWLRQ LQ WKH 6FLHQWLVWV$ZDUGIRU%LRFKHPLVWU\ IURPLWV+RUL]RQSURJUDPPHWR PROHFXODUOLIHVFLHQFHVDQGVWUXFWXUDO DQG %LRSK\VLFV IRU KHU UHVHDUFK LQWR D SURMHFW FRRUGLQDWHG E\ 5ROI %RH ELRORJ\7KLVKDVDOVRUHVXOWHGLQQX WKH ZRUNLQJV RI WKH SURWHDVRPH D OHQVFDOOHGL1(;7WRSURYLGHDFFHVV PHURXVDZDUGHGJUDQWVDQGWKHDGGL VWUXFWXUH WKDW EUHDNV GRZQ SURWHLQV WR VWUXFWXUDO ELRORJ\ WHFKQRORJLHV WLRQRI QHZH[SHUWLVHWRWKH&HQWHU LQ ELRORJLFDOFHOOV ,Q DGGLWLRQ 0DUF WRRWKHUUHVHDUFKHUVLQ(XURSH$QG %DOGXV UHFHLYHG WKH *QWKHU /DXN WKHUHZHUHPDQ\PDQ\PRUHJUDQWV (DUO\ SURI &DVSHU +RRJHQ LHQ 3UL]H WKDW UHFRJQL]HV FXWWLQJ DZDUGHGWRWKH%LMYRHW&HQWHULQWKLV UDDG DQG SURI $QQD $NKPDQRYD RI HGJHH[SHULPHQWDOUHVHDUFKLQ105 SHULRGDVGHVFULEHGLQWKLVUHSRUW WKH 'HSDUWPHQW RI %LRORJ\ EHFDPH LQ ,Q $QWRLQHWWH .LOOLDQ DVVRFLDWH PHPEHUV RI WKH %LMYRHW ZDVDZDUGHGWKHSUHVWLJLRXV(%6$ :LWKDVWURQJUHFHQWSDVWOHW·VZRUN &HQWHU,QWKHVDPH\HDUSURI*HHUW $YDQWL SUL]H IRU RXWVWDQGLQJ FRQWUL RQ PDNLQJ WKH FRPLQJ \HDUV DQ -DQ%RRQVDJO\FRPLFVDQGFKHPLFDO EXWLRQV WR WKH XQGHUVWDQGLQJ RI OL RWKHUPLOHVWRQHLQWKHKLVWRU\RI RXU ELRORJ\H[SHUWZDVDSSRLQWHGMRLQWO\ SLG ELRSK\VLFV ,Q $XJXVW WKH FHQWHU ZLWK WKH 8WUHFKW ,QVWLWXWH IRU 3KDU $PHULFDQ&KHPLFDO6RFLHW\KRQRXUHG PDFHXWLFDO6FLHQFHVDVDQHZSURIHV $OEHUW+HFNZLWKWKH¶$&6)UDQN+ VRUDW8WUHFKW8QLYHUVLW\7KLV\HDU )LHOGDQG-RH/)UDQNOLQ$ZDUGIRU SURI)ULHGULFK)|UVWHUDQGGU7VYL\D 2XWVWDQGLQJ $FKLHYHPHQWV LQ 0DVV =HHY%HQ0RUGHKDL WZR H[SHUWV 6SHFWURPHWU\· DQG SUHVHQWHG WKH LQ FU\RHOHFWURQ PLFURVFRS\ ZLOO ¶$UWKXU & &RSH 6FKRODU $ZDUG· WR MRLQRXUFHQWHU7KHVHQHZDGGLWLRQV *HHUW-DQ%RRQV(DUO\&DVSHU WR WKH %LMYRHW &HQWHU ZLOO IXUWKHU +RRJHQUDDG ZDV DZDUGHG WKH VWUHQJWKHQRXUVFLHQWLILFSRVLWLRQDQG ,%52.HPDOL,QWHUQDWLRQDO3UL]HIRU WKHILUVWFROODERUDWLYHSXEOLFDWLRQVDUH 5HVHDUFK LQ WKH )LHOG RI %DVLF DQG DOUHDG\HPHUJLQJ &OLQLFDO1HXURVFLHQFH Prof. dr. Marc Baldus Dr. Reinout Raijmakers 6FLHQWL¿F'LUHFWRU 0DQDJLQJ'LUHFWRU Bijvoet Center for Biomolecular Research 1 Contact Details Postal address 6FLHQWL¿F$GYLVRU\%RDUG Bijvoet Center for Biomolecular Research Padualaan 8 Prof.dr. J.F.G. Vliegenthart (chairman) Utrecht University Emeritus of Utrecht University, NL Padualaan 8 (room O604) Prof.dr. A. Bax 3584 CH Utrecht NIH, Bethesda, USA The Netherlands Prof.dr. J. Johnson, The Scripps Research Institute, La Jolla, USA Prof.dr. K. Wilson Contact details University of York, UK Dr. Reinout Raijmakers Managing Director Bijvoet Center for Biomolecular Research Visiting addresses Tel.: +31 30 253 6804 E-mail: [email protected] .UX\WJHERXZ [email protected] Padualaan 8 3584 CH Utrecht Website Bijvoet Center The Netherlands www.bijvoet-center.eu %ORHPEHUJHQJHERXZ Enter via Kruytgebouw Padualaan 8 3584 CH Utrecht The Netherlands 'DYLGGH:LHGJHERXZ Website PhD programme MLS Universiteitsweg 99 www.bijvoet-center.eu/phd-programme/ 3484 CG Utrecht The Netherlands Executive Board Prof. dr. M. Baldus (Scientific Director) Prof. dr. A.J.R. Heck Stratenum Prof. dr. P. Gros Prof. dr. I. Braakman Universiteitsweg 100 Prof. dr. A. Killian 3584 CG Utrecht Prof. dr. G.J. Boons The Netherlands Dr. R. Raijmakers (Managing Director) Colofon Design by Reinout Raijmakers, photography by Ivar Pel, Bas van Breukelen, Reinout Raijmakers, and others. 2 Bijvoet Center for Biomolecular Research Bijvoet Center for Biomolecular Research 3 Organization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iomolecular Mass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² WKH 'HSDUWPHQWV RI &KHPLVWU\ DQG IURP¶FHOOVWRDWRPV·²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|UVWHUZLOOVWDUWDVWKHQHZKHDGRI 7KH &U\VWDO DQG 6WUXFWXUDO &KHP EUDQHV DUH LQYROYHG LQ WKH PRGH RI WKH&U\R(0JURXS7KHJURXSIXU LVWU\ JURXS SHUIRUPV UHVHDUFK LQWR DFWLRQ RI GUXJV WR[LQV DQWLELRWLFV WKHU LQFOXGHV SULQFLSDO LQYHVWLJDWRUV WKH WKUHHGLPHQVLRQDO VWUXFWXUHV RI DQG DP\ORLG IRUPLQJ SURWHLQV 3ULQ :DOO\0OOHUDQG:LOOLH*HHUWV PROHFXOHVZKLFKGHILQHWKHPROHFXODU FLSDO LQYHVWLJDWRUV LQ WKLV JURXS DUH LQWHUDFWLRQVDQGUHDFWLRQVWKDWXQGHU $QWRLQHWWH .LOOLDQ 7RRQ GH .URRQ Timmers group OLH FRPSOH[ FKHPLFDO DQG ELRORJLFDO DQG(HIMDQ%UHXNLQN 7KH UHVHDUFK RI 0DUF 7LPPHUV IR SURFHVVHV 7KH PDLQ UHVHDUFK OLQHV FXVHVRQWKHLQWHUSOD\EHWZHHQFKUR DUHLQWKHILHOGRI SURWHLQFU\VWDOORJ &HOOXODU3URWHLQ&KHPLVWU\
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