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Proceedings of the Third Symposium of The PROCEEDINGS OF THE THIRD SYMPOSIUM OF (!r' THE INTERNATIONAL WORKING GROUP ON r PLANT VIRUSES WITH FUNGAL VECTORS it Editors: J.L. Sherwood and C.M. Rush West Park Conference Centre, Dundee, Scotland August 6-7, 1996 ISBN 0-92619502-6 1996 American Society of Sugar Beet Technologists 800 Grant, Suite 500, Denver, CO 80203 Printed in U.S.A. PREFACE The International Working Group on Plant Viruses with Fungal Vectors IIWGPVFV) was formed in 1988 at Kyoto, Japan, with Dr. Chuji Hiruki as the chairperson. The goal of the working group is to provide a forum to facilitate international collaboration and multidisciplinary research on plant viruses with fungal vectors. Thus, topics at symposia have included a) biology of viruses with fungal vectors, b) biology of fungi that transmit plant viruses, c) interaction between these viruses and vectors, and d) epidemiology and control of diseases caused by plant viruses transmitted by soilborne fungi. Symposia of the working group have been held at the Biologische Bundesanstalt (BBA) in Braunschweig, Germany (19901, McGill University in Montreal, Canada (1993) and, most recently, at The West Park Conference Centre, University of Dundee, Dundee, Scotland (1996). This volume serves as a record of material presented at this most recent meeting for use by members of the IWGPVFV and for those with an interest in the activities of the IWGPVFV. As the IWGPVFV is a totally volunteer group, the success of its meetings is a result of the hard work and contributions of the local organizing committee and sponsors. Those responsible for the success of the most recent meeting are listed on the next page. The names and e-mail addresses of the current program committee are also listed. Please contact a member of the program committee if you wish to be included in any future mailings of the IWGPVFV. The next symposium is scheduled for 1999. John L. Sherwood Chairperson, IWGPVFV SPONSORS LOCAL ARRANGEMENTS Stratagene Cloning Systems Mrs. Fern Watt Stratagene Limited Dr. Lesley Torrance 140 Cambridge Science Park Milton Road Cambridge CB4 4GFR, UK Members of the Virology staff at SCRl Adgen SAC Diagnostic Systems for assistance during the meeting. Watson Peat Building Auchincruive PROGRAM COMMITTEE Ayr KA6 5HW, UK Dr. Mike dams The Scottish Seed Potato Development [email protected]. uk Council Dr. Charlie Rush 4 Brewery Court cm-rush@tamu. edu Haddington Dr. John Sherwood EH41 4DG, UK plpajls@osuunx. ucc. oksta re. edu Tesco Stores Ltd SESSION CHAIRPERSONS PO Box 73 Baird Avenue Characterization I Dryburgh Industrial Estate C.M. Rush, G.C. Wisler Dundee Characterization II DO1 9NF, UK E.J. Cartwright, R.H.A. Coutts The Gatsby Charitable Foundation Mycology 9 Red Lion Court R.N. Campbell, E. Ward London Resistance EC4A 3EB, UK J. Chen, J.L. Sherwood Scotlab Sequence and Genome Analysis I Kirkshaws Road B. Reavy, L. Torrance Coatbridge Detection Lanarkshire ML5 8AD, UK U. Merz, J.L. Sherwood British Sugar plc Epidemiology Holrnewood Hall M.J. Adams, P. Delfosse Holrne Sequence and Genome Analysis II Peterborough PE7 3PG, UK M.A. Mayo, R.A. Naidu Bio-Rad Laboratories Ltd Bio-Rad House Maylands Avenue Hemel Hempstead Herts HP2 7TD, UK Contents Page Preface ............................................................................................................................ .3 Acknowledgements ............................................................................................................ 4 Contents ........................................................................................................................... 5 Characterization Barley mild mosaic virus: deletions, duplications and transmission. .......................................... 9 E.J. Cartwright, A. Rosner, E. Peerenboom, H.-H. Steinbiss, J.F. Antoniw and M. J. Adams, Synthesis, expression and location of the tobacco necrosis Necrovirus strain D p7a movement protein ............................................................................................ 13 R.H.A. Coutts and S.K. Offei The localization of the functional sequence on RNA4 of beet necrotic yellow vein virus (BNYVV) related to fungus transmission by inoculation with infectious RNA4 and its mutants transcripted in vitro. ....................................................17 C.G. Han, D.Y. Wang, J.L. Yu, D.W. Li, L.L. Yang, Z.N. Cai and Y. Liu The genome organization and RNA sequence of cucumber leaf spot virus, a tombus-like virus. ...................................................................................................21 J.S. Miller, H. Damude, M.A. Robbins, R.D. Reade and D.M. Rochon Characterization of a poorly transmissible cucumber necrosis virus mutant. .............................25 M.A. Robbins, R.D. Reade, and D.M. Rochon Similarities between beet soilborne mosaic virus and beet necrotic yellow vein virus RNAZ nucleotide sequence and genomic organization. ............................................29 C.M. Rush, K.-B.G. Scholthof, S.K. Manohar and G.B. Heidel BNYVV readthrough domain analysis: a KTER motif is important for transmission of the virus by Polymyxa betae .........................................................................33 C. Schmitt, T. Tamada, M. Saito, H. Guilley, K. Richards and G. Jonard Characterisation of UK isolates of barley yellow mosaic virus. ................................................37 Nongnong Shi, M.J. Adams, Jianping Chen, T.M.A. Wilson, S.A. MacFarlane and J.F. Antoniw Strain differences between isolates of barley yellow mosaic virus in China ............................... 41 Nongnong Shi, Jianping Chen, M.J. Adams, Fengtai Zhu, Zhiqiang Wang, T.M.A. Wilson, S.A. MacFarlane and J. F. Antoniw Analysis of RNA1 of wheat spindle streak mosaic bymovirus (WSSMV). ................................. 45 A. Sohn, S. Leclair and H.-H. Steinbit3 Evidence that beet necrotic yellow vein virus RNA-5 is involved in symptom development of sugar-beet roots ..........................................................................................49 T. Tamada, T. Kusume, H. Uchino, T. Kiguchi and M. Saito Comparative molecular analyses of several BNYVV and BSBMV-related furoviruses infecting sugarbeet. ...........................................................................................53 G.C. Wisler, H.-Y. Liu and J.E. Duffus Sequence and genome analysis Studies on potato mop-top virus replication ..........................................................................57 M. Arli, B. Reavy and L. Torrance Evidence that readthrough of the potato mop-top virus coat protein gene occurs in plants and that the readthrough domain is present at one extremity of some particles. ................................................................................................61 G.H. Cowan, L. Torrance, and B. Reavy Nucleotide sequences of beet soil-borne virus RNAs 2 and 3. .................................................65 Renate Koenig, I-llrich Commandeur and Andrea Kaufmann The genome organization of broad bean necrosis virus (BBNV) and heterogeneity of the genus Furovirus. ..................................................................................69 Lu Xiaoyun, Yamamoto Shinya, Tanaka Yuzuru, Hibi Tadaaki and Namba Shigetou The nucleotide sequence of RNA-1 of Indian peanut clump virus complicates its taxonomy but offers broad spectrum diagnostics. .............................................................13 J.S. Miller, S.V. Wesley, R.A. Naidu, D.V.R. Reddy and M.A. Mayo The nucleotide sequence of Indian peanut clump virus RNA 2 .................................................77 R.A. Naidu, J.S. Miller, M.A. Mayo and A.S. Reddy Complete nucleotide sequence of wheat yellow mosaic bymovirus genomic RNAs. ..................81 Shigetou Namba and Satoshi Kashiwazaki Mycology Fungal vectors in the tombusviridae. ....................................................................................85 R.N. Campbell Factors affecting the release of primary zoospores from cystosori of Spongospora subterranea assessed using monoclonal antibody ELlSA test. ..........................89 N. Fornier, A.A. Powell and P.J. Burgess Molecular studies of variation amongst isolates of Polymyxa graminis and Polymyxa betae. ..........................................................................................................93 E. Ward and M.J. Adams Cytology Ultrastructural studies of resting spore development in Polymyxa graminis ...............................97 Jianping Chen, Zhiqiang Wang, Jian Hong, C.R. Collier and M.J. Adams Watercress yellow spot virus (WYSV): cytopathic alterations suggest it is a member of the tombusviridae ...........................................................................................101 C.M. Clay and J.A. Walsh Microscopical observations on release and morphology of and host infection by primary zoospores of Spongospora subterranea f .sp. subterranea .......................................I05 U. Merz The p37 of soilborne wheat mosaic virus (SBWMV) moves from cell to cell in wheat via plasmodesmata ...................................................................................................109 X. Zhu, B. Ding, and J.L. Sherwood Detection, etiology and control Effects of cultivar and sowing date on the incidence of barley mosaic viruses and on yield. ......................................................................................................................1 13 M.J. Adams. R. Overthrow and M.F.F. Carver The presence of Spongospora subterranea f.sp. subterranea in
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