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Zinc and Cadmium in Paper (Reaffirmation of T 438 Cm-96)
WI 050114.01 T 438 DRAFT NO. 5 DATE July 27, 2006 TAPPI WORKING GROUP CHAIRMAN J Ishley SUBJECT CATEGORY Fillers & Pigments Testing RELATED METHODS See “Additional Information” CAUTION: This Test Method may include safety precautions which are believed to be appropriate at the time of publication of the method. The intent of these is to alert the user of the method to safety issues related to such use. The user is responsible for determining that the safety precautions are complete and are appropriate to their use of the method, and for ensuring that suitable safety practices have not changed since publication of the method. This method may require the use, disposal, or both, of chemicals which may present serious health hazards to humans. Procedures for the handling of such substances are set forth on Material Safety Data Sheets which must be developed by all manufacturers and importers of potentially hazardous chemicals and maintained by all distributors of potentially hazardous chemicals. Prior to the use of this method, the user must determine whether any of the chemicals to be used or disposed of are potentially hazardous and, if so, must follow strictly the procedures specified by both the manufacturer, as well as local, state, and federal authorities for safe use and disposal of these chemicals. Zinc and cadmium in paper (Reaffirmation of T 438 cm-96) (no changes were made since last draft) 1. Scope and significance 1.1 This method maybe used for the determination of cadmium and zinc either in paper or in highly opaque pigments. Zinc is usually present in zinc oxide, zinc sulfide, or as lithopone (a combination of zinc sulfide and barium sulfate), which is occasionally used in filled paper, in paper coatings and in high-pressure laminates and wallpaper. -
Npgrj Nprot 406 2517..2526
PROTOCOL Identification and analysis of essential Aspergillus nidulans genes using the heterokaryon rescue technique Aysha H Osmani, Berl R Oakley & Stephen A Osmani Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, USA. Correspondence should be addressed to S.A.O. ([email protected]) Published online 29 December 2006; corrected online 25 January 2007 (details online); doi:10.1038/nprot.2006.406 s In the heterokaryon rescue technique, gene deletions are carried out using the pyrG nutritional marker to replace the coding region of target genes via homologous recombination in Aspergillus nidulans. If an essential gene is deleted, the null allele is maintained in spontaneously generated heterokaryons that consist of two genetically distinct types of nuclei. One nuclear type has the essential gene deleted but has a functional pyrG allele (pyrG+). The other has the wild-type allele of the essential gene but lacks a functional pyrG allele (pyrG–). Thus, a simple growth test applied to the uninucleate asexual spores formed from primary transformants can natureprotocol / m identify deletions of genes that are non-essential from those that are essential and can only be propagated by heterokaryon rescue. o c . The growth tests also enable the phenotype of the null allele to be defined. Diagnostic PCR can be used to confirm deletions at the e r molecular level. This technique is suitable for large-scale gene-deletion programs and can be completed within 3 weeks. u t a n . w w INTRODUCTION w / / : One of the most fundamental pieces of information regarding the non-essential gene is deleted, the resulting strains are able to grow p t t function of any gene is whether the gene is essential or not. -
Detection of Acid-Producing Bacteria Nachweis Von Säureproduzierenden Bakterien Détection De Bactéries Produisant Des Acides
(19) TZZ ¥ _T (11) EP 2 443 249 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.: of the grant of the patent: C12Q 1/04 (2006.01) G01N 33/84 (2006.01) 19.11.2014 Bulletin 2014/47 (86) International application number: (21) Application number: 10790013.6 PCT/US2010/038569 (22) Date of filing: 15.06.2010 (87) International publication number: WO 2010/147918 (23.12.2010 Gazette 2010/51) (54) DETECTION OF ACID-PRODUCING BACTERIA NACHWEIS VON SÄUREPRODUZIERENDEN BAKTERIEN DÉTECTION DE BACTÉRIES PRODUISANT DES ACIDES (84) Designated Contracting States: (74) Representative: Isarpatent AL AT BE BG CH CY CZ DE DK EE ES FI FR GB Patent- und Rechtsanwälte GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO Friedrichstrasse 31 PL PT RO SE SI SK SM TR 80801 München (DE) (30) Priority: 15.06.2009 US 187107 P (56) References cited: 15.03.2010 US 314140 P US-A- 4 528 269 US-A- 5 098 832 US-A- 5 164 301 US-A- 5 601 998 (43) Date of publication of application: US-A- 5 601 998 US-A- 5 786 167 25.04.2012 Bulletin 2012/17 US-B2- 6 756 225 US-B2- 7 150 977 (73) Proprietor: 3M Innovative Properties Company • DARUKARADHYA J ET AL: "Selective Saint Paul, MN 55133-3427 (US) enumeration of Lactobacillus acidophilus, Bifidobacterium spp., starter lactic acid bacteria (72) Inventors: and non-starter lactic acid bacteria from Cheddar • YOUNG, Robert, F. cheese", INTERNATIONAL DAIRY JOURNAL, Saint Paul, Minnesota 55133-3427 (US) ELSEVIER APPLIED SCIENCE, BARKING, GB, • MACH, Patrick, A. -
FS/2018 ORGANIC CHEMISTRY I LAB CHEM 226 (2219) Instructor
FS/2018 ORGANIC CHEMISTRY I LAB CHEM 226 (2219) Instructor: Terry Bone 120-B Schrenk Hall [email protected] 341-4820 http://www.mst.edu/~tbone 9:00-10:00, Tu-Th, or by appt. Cynthia Bolon 213 Schrenk Hall [email protected] 341-4439 OBJECTIVES: Course objectives are to develop facility with performing laboratory techniques involving the handling of organic chemicals safely and the keeping of proper records of experiments conducted in the laboratory. Crystallization, distillation, extraction and chromatography are emphasized as separation and purification techniques. Melting points, boiling points, and refractive indices are used routinely as measures of purity. Safe handling of chemicals and proper disposal of waste products are priority goals in this course. To help in attaining such goals, the first week of the semester is devoted to SAFETY in the chemistry laboratory. For the remainder of the semester, most of the experiments are conducted on a micro scale. BOOKS: 1. 100 pg. set Organic Chemistry Laboratory Notebook 2. “Organic Chemistry Laboratory Laboratory Techniques, 2nd ed”. Available as free pdf download at https://open.umn.edu/opentextbooks/BookDetail.aspx?bookId=369 3. “Microscale Techniques for the Organic Laboratory, 2nd ed”, (MTOL), Mayo, Pike, Butcher and Trumper, John Wiley & Sons, Inc.,2001 (on reserve at library circulation desk-currently out of print but available on Amazon) 4. “Right to Know pocket guide for School & University Employees”, Genium Publishing Corp., 1990. (optional) (#1 can be purchased from the book store) LAB SYLLABUS: Originally prepared by Prof. S. B. Hanna, modified by D. E. Hoiness, T.Bone. GENERAL GUIDELINES SAFETY Goggles must be worn at all times in the lab. -
EWRE Lab Regulations
Department of Civil & Environmental Engineering University of Massachusetts College of Engineering University of Massachusetts September 2012 CONTENTS UNIVERSITY OF MASSACHUSETTS ......................................................................................................................... 1 CONTENTS ...................................................................................................................................................................... 2 A. LABORATORY SAFETY AND SECURITY ........................................................................................................... 1 1. GENERAL RULES .......................................................................................................................................................... 1 2. EMERGENCY ................................................................................................................................................................ 2 3. DISPOSAL OF CHEMICALS ............................................................................................................................................ 2 a. What chemical wastes are hazardous or non-hazardous? ...................................................................................... 2 b. Disposal of hazardous wastes ................................................................................................................................. 2 4. FUME HOODS .............................................................................................................................................................. -
Harvard Biolabs Stockroom
THE HARVARD BIOLABS STOCKROOM Biolabs Basement—B060 Biolabs Bldg ‐ 16 Divinity Ave Phone: 617.495.2385 Monday ‐ Friday: 8:00 am ‐ 4:50 pm* *Closed daily 12:00 ‐ 1:00 pm The Harvard Biolabs Stockroom The Harvard BioLabs Stockroom Biolabs Basement—B060 The current Harvard Biolabs Stockroom was created in collaboraon with Operaons and Facilies at FAS and VWR Internaonal to provide researchers with quick, convenient access to the most frequently ordered laboratory supplies, enzymes and biologicals. Products are sourced from VWR core suppliers, as well as other popular manufacturers such as Qiagen, NEB, Falcon and Corning. Researchers using Harvard funds for payment are eligible to purchase from the Stockroom. To ensure accurate billing, shoppers should be prepared to provide idenficaon and up‐to‐date grant informaon at the request of Stockroom personnel. All non‐stockroom orders should be placed on‐line via HCOM or by calling VWR at 866‐229‐9967 “Call Ahead” ‐The Stockroom offers walk‐ in service. However, you may want to “call ahead” to 617‐495‐2385 so the VWR associate can ensure availability of the products requested. VWR will provide a receipt detailing all items on the order. Only products in stock will be included. Back orders will not be accepted. Backordered products should be ordered as a new transacon when stock arrives. Check with VWR at 866‐229‐9967 or HCOM for availability. VWR manages the 190 and 200 proof tax free ethanol program for Harvard University. Ethanol can be purchased by the gallon(s) or pint in the Stockroom. The on‐campus stockroom is intended to meet immediate needs for less‐ than‐case quanty products; for larger orders it is recommended purchases be made on line via HCOM, by calling 866‐229‐9967 or by e‐mailing [email protected]. -
Photograpmc MATERIALS CONSERVATION CATALOG
PHOTOGRAPmC MATERIALS CONSERVATION CATALOG The American Institute for Conservation of Historic and Artistic Works Photographic Materials Group FIRST EDmON November 1994 INPAINTING OUTLINE The Pbotographlc MaterIals CODServatioD Catalog is a publication of the Photographic Materials Group of the American Institute for CODBervation of Historic and Artistic Works. The Photographic MaterIals CoDServatioD Catalog is published as a convemence for the members of the Photographic Materials Group. Publication in DO way endorses or recommends any of the treatments, methods, or techniques described herein. First Edition copyright 1994. The Photographic Materials Group of the American Institute for CODBervation of Historic and Artistic Works. Inpa........ 0utIIDe. Copies of outline chapters of the Pbotograpble MaterIals CoaservatloD Catalog may be purchased from the American Institute for CODBervation of Historic and Artistic Works, 1717 K Street, NW., Suite 301, Washington, DC 20006 for $15.00 each edition (members, $17.50 non-members), plus postage. PHOTOGRAPIDC MATERIALS CONSERVATION CATALOG STATEMENT OF PURPOSE The purpose of the Photograpbic Materials Conservation Catalog is to compile a catalog of coDSe1'Vation treatment procedures and information pertinent to the preservation and exhibition of photographic materials. Although the catalog will inventory techniques used by photographic conservators through the process of compiling outlines, the catalog is not intended to establish definitive procedures nor to provide step-by-step recipes for the untrained. Inclusion of information in the catalog does not constitute an endorsement or approval of the procedure described. The catalog is written by conservators for CODSe1'Vators, as an aid to decision making. Individual conservators are solely responsible for determining the safety, adequacy, and appropriateness of a treatment for a given project and must understand the possible effects of the treatment on the photographic material treated. -
A Re-Evaluation of the Filter Paper Method of Measuring
A RE-EVALUATION OF THE FILTER PAPER METHOD OF MEASURING SOIL SUCTION by RIFAT BULUT, B.S.C.E. A THESIS IN CIVIL ENGINEERING Submitted to the Graduate Faculty of Texas Tech University in Partial Fulfillment of the Requirements for the Degree of MASTER OF SCIENCE IN CIVIL ENGINEERING Approved August, 1996 Eos I ^^^ ACKNOWLEDGMENTS UhO 1^ would like to express my sincere and deep appreciation to Dr. Warren Kent Wra> for his guidance, endless encouragement, and assistance throughout the course of this study. I also wish to thank Dr. Priyantha W. Jayawickrama for his kindK consentmg to serve on my thesis committee. 1 would sincerely like to thank Mr. Hsiu-Chung Lee for his wholehearted cooperation during performing of the experiments and guidance. My sincere appreciation to Mr. Brad Thomhill, Mario Torres, and Drex Little for their help in providing equipment for the experiments. Finally, 1 wish to thank my family and friends for their support and encouragement throughout the whole study period. n TABLE OF CONTENTS ACKNOWLEDGMENTS ii ABSTRACT v LIST OF TABLES vi LIST OF FIGURES vii CHAPTER L INTRODUCTION 1 1.1 Problem Statement 1 1.2 Statement of Objectives 4 1.3 Research Approach 6 11. SEARCH OF THE TECHNICAL LITERATURE 7 2.1 Soil Suction Concept 7 2.2 The Filter Paper Method 14 2.2.1 Historical Background ofthe Filter Paper Calibration 15 2.2.2 Working Principle ofthe Filter Paper Method 22 2.2.2.1 Principle of Total Suction Measurements 23 2.2.2.2 Principle of Matric Suction Measurements 25 2.2.3 Calibration Technique of Filter Paper 26 2.2.3.1 Total Suction Calibration 26 2.2.3.2 Matric Suction Calibration 29 2.2.4 Performance of Filter Paper Method 33 m. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Bacterial Survival in Microscopic Surface Wetness Maor Grinberg†, Tomer Orevi†, Shifra Steinberg, Nadav Kashtan*
RESEARCH ARTICLE Bacterial survival in microscopic surface wetness Maor Grinberg†, Tomer Orevi†, Shifra Steinberg, Nadav Kashtan* Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University, Rehovot, Israel Abstract Plant leaves constitute a huge microbial habitat of global importance. How microorganisms survive the dry daytime on leaves and avoid desiccation is not well understood. There is evidence that microscopic surface wetness in the form of thin films and micrometer-sized droplets, invisible to the naked eye, persists on leaves during daytime due to deliquescence – the absorption of water until dissolution – of hygroscopic aerosols. Here, we study how such microscopic wetness affects cell survival. We show that, on surfaces drying under moderate humidity, stable microdroplets form around bacterial aggregates due to capillary pinning and deliquescence. Notably, droplet-size increases with aggregate-size, and cell survival is higher the larger the droplet. This phenomenon was observed for 13 bacterial species, two of which – Pseudomonas fluorescens and P. putida – were studied in depth. Microdroplet formation around aggregates is likely key to bacterial survival in a variety of unsaturated microbial habitats, including leaf surfaces. DOI: https://doi.org/10.7554/eLife.48508.001 Introduction *For correspondence: The phyllosphere – the aerial parts of plants – is a vast microbial habitat that is home to diverse [email protected] microbial communities (Lindow and Brandl, 2003; Lindow and Leveau, 2002; Vorholt, 2012; Vacher et al., 2016; Leveau, 2015; Bringel and CouA˜ ce, 2015). These communities, dominated by †These authors contributed bacteria, play a major role in the function and health of their host plant, and take part in global bio- equally to this work geochemical cycles. -
Advantec Mfs, Inc
CONTENTS TABLE OF CONTENTS i INTRODUCTION ii HOW TO ORDER iii MEMBRANE FILTERS 1 MICROBIOLOGY SUPPLIES 17 LABORATORY FILTER PAPERS 25 SPECIALTY PRODUCTS 35 TEST PAPERS 41 INDUSTRIAL FILTER PAPERS & PADS 45 CAPSULES AND CARTRIDGES 53 VACUUM FILTRATION 83 PRESSURE FILTRATION 103 APPENDIX/INDEX 125 FILTRATION MEDIA & FILTRATION SYSTEMS CATALOG•Volume15 ADVANTEC MFS, INC. 6723 Sierra Court, Suite A Dublin, California 94568 U.S.A. Phone (800) 334-7132 +1-925-479-0625 Fax +1-925-479-0630 E-mail [email protected] URL http://www.advantecmfs.com TRADEMARK ADVANTEC is trademark/registered trademark in Japan and other countries of Toyo Roshi Kaisha, Ltd. and its group companies. TABLE OF CONTENTS MEMBRANE FILTERS pH Test Paper-Strips . .43 VACUUM FILTRATION Introduction . .2 Litmus Paper-Booklets . .44 Introduction . .84 Properties of Membrane Filters . .3 Ion Test Papers . .44 Glass Microfiltration: Support Systems . .85 Mixed Cellulose Esters (MCE) . .4 Chlorine Test Papers . .44 13 mm Glass Microanalysis Holders . .86 Cellulose Acetate . .7 25 mm Glass Microanalysis Holders . .87 Hydrophobic PTFE . .8 INDUSTRIAL FILTER PAPERS & PADS 47 mm Glass Microanalysis Holders . .88 Hydrophobic PTFE with Standard Filter Papers . .46 47 mm Glass Microanalysis Holders Polypropylene Net Support . .9 Fine Particle Filter Papers . .46 – With All-PTFE Seal . .89 Hydrophilic PTFE . .10 Creped Filter Papers . .46 47 mm Glass Sterility Test Unit . .90 Coated Cellulose Acetate . .11 Wet Strength Filter Papers . .47 90 mm Glass Microanalysis Holders . .91 Nylon . .12 High Purity Filter Papers . .48 Filter Flasks and Stoppers . .92 Polycarbonate . .13 High Viscosity Fluid Filter Papers . .48 All-Glass Filtration Assemblies . .93 Prefilters for Membrane Filters . -
Laboratory Manual
International Program UAM-Boston University Laboratory Manual Organic Chemistry I 2013-2014 Departamento de Química Orgánica Ernesto Brunet Romero Ana María Martín Castro Ramón Gómez Arrayás Laboratory Manual Table of Contents ............................................................................... 1 Introduction ............................................................................... 2 Prelab preparation ............................................................................... 2 Notebook ............................................................................. 3 Safety .............................................................................. 3 Laboratory Practices and Safety Rules ............................................................. 4 Accidents and injuries ........................................................................... 5 Fires ............................................................................. 5 Chemical Wastes ............................................................................. 6 Cleaning Responsibilities ............................................................................. 6 Lab cleanliness ............................................................................. 6 Laboratory Equipment ............................................................................. 7 Proper use of glassware ............................................................................. 8 Some techniques in lab experiments Heating, cooling and stirring ............................................................................