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RAD23A Antibody Cat RAD23A Antibody Cat. No.: 29-716 RAD23A Antibody Antibody used in IHC on Human Lung at 4.0-8.0 ug/ml. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Dog, Human, Mouse, Rat Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human RAD23A. TESTED APPLICATIONS: ELISA, IHC, WB RAD23A antibody can be used for detection of RAD23A by ELISA at 1:62500. RAD23A APPLICATIONS: antibody can be used for detection of RAD23A by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate PREDICTED MOLECULAR 40 kDa WEIGHT: September 25, 2021 1 https://www.prosci-inc.com/rad23a-antibody-29-716.html Properties PURIFICATION: Antibody is purified by protein A chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: RAD23A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: RAD23A ALTERNATE NAMES: RAD23A, HHR23A, MGC111083, HR23A ACCESSION NO.: NP_005044 PROTEIN GI NO.: 4826964 GENE ID: 5886 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References RAD23A is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.The protein encoded by BACKGROUND: this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. REFERENCES: 1) Ju, D. (2006) Biochem. Biophys. Res. Commun. 341 (2), 648-652. ANTIBODIES FOR RESEARCH USE ONLY. September 25, 2021 2 https://www.prosci-inc.com/rad23a-antibody-29-716.html For additional information, visit ProSci's Terms & Conditions Page. September 25, 2021 3 https://www.prosci-inc.com/rad23a-antibody-29-716.html.
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