PLOS COMPUTATIONAL BIOLOGY EDUCATION Ten quick tips for homology modeling of high- resolution protein 3D structures 1,2 1,2 1,2 Yazan HaddadID , Vojtech AdamID , Zbynek HegerID * 1 Department of Chemistry and Biochemistry, Mendel University in Brno, Brno, Czech Republic, 2 Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic *
[email protected] Author summary The purpose of this quick guide is to help new modelers who have little or no background in comparative modeling yet are keen to produce high-resolution protein 3D structures a1111111111 for their study by following systematic good modeling practices, using affordable personal a1111111111 computers or online computational resources. Through the available experimental 3D- a1111111111 structure repositories, the modeler should be able to access and use the atomic coordinates a1111111111 for building homology models. We also aim to provide the modeler with a rationale a1111111111 behind making a simple list of atomic coordinates suitable for computational analysis abiding to principles of physics (e.g., molecular mechanics). Keeping that objective in mind, these quick tips cover the process of homology modeling and some postmodeling computations such as molecular docking and molecular dynamics (MD). A brief section OPEN ACCESS was left for modeling nonprotein molecules, and a short case study of homology modeling Citation: Haddad Y, Adam V, Heger Z (2020) Ten is discussed. quick tips for homology modeling of high- resolution protein 3D structures. PLoS Comput Biol 16(4): e1007449. https://doi.org/10.1371/ journal.pcbi.1007449 Introduction Editor: Francis Ouellette, University of Toronto, Protein 3D-structure folding from a simple sequence of amino acids was seen as a very difficult CANADA problem in the past.