Scientific Report 2010-2011

CETFCREPORT SCIENTIFIC

201 2011 – 0

PENNINGTON BIOMEDICAL RESEARCH CENTER RESEARCH BIOMEDICAL PENNINGTON SCIENTIFIC REPORT | 2010–2011

6400 Perkins Road Baton Rouge, Louisiana 70808 Telephone: 225-763-2500 www.pbrc.edu OUR SUPPORTERS 129

* Deceased

010–2011 2

Contractors, LLC Contractors, Group, LLC Group, MichaelRood Mr. Foundation Family Consultants, LLC Ralph and Margi Underwood Margi and Ralph Research Corporate Unilever ChannelWAFB-TV 9 Jr. Nancy and Gerald L. Walter, Inc. International, Watchers Weight Willis-Knighton Health System Winkler Anne and David Bobby and Marsha Yarborough and JohnJanet A. Young Phelps Dunbar, LLP Phelps Dunbar, Mary Pierson Olive Poche’ Shannon and Jim Proctor & Gamble Company EstateReal Services Management LLC Rouge, AssociatesRenal of Baton Ragan and Virginia Richard Jennifer C. Rood and Dr. Beverly and Rory Russell and Mrs.Mr. Sammons Chris Schwab Charitable Fund and MarcyFrank Simoneaux Christel C. and William S. III Slaughter Mrs. and Solomon, Solomon Lee Mr. Southeastern Cardiovascular Stuart General & Company SCIENTIFIC REPORT SCIENTIFIC Mr. and Mrs. Kissam Luther IV Mr. Kraft Foods, Inc. Land O’Lakes Purina Feed Isabel and Clifton C. Lasseigne, Jr. LeBlanc Gordon and Teri Mrs. Jane R. LeBlanc JewelryLee Fine Michaels LEMIC Company Insurance and Alex Lewis CC Institute Health Public Louisiana Anonymous *Mrs. Garvey Paula Manship Mary Beth and Michael H. Martin Supermarkets Matherne’s Inc. McCormick & Company, Rouge of Baton McDonald’s McIlwain and Terri Jim and RobertPriss H. McNeese, Sr. Henry S. Miller K. and Penny Inc. Morgan Keegan and Company, Morgan Stanley Jr. Moyse, *Hermann and Leonard R.Julie II Nachman Nestle’ S.A. Orexigen Therapeutics, Inc. Parnell Harrison Mrs. Ann Ruth M. Patrick Dr. Institute The Peanut Inc. PepsiCo, and Cherie Peters Bill Corporation Petrin

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Sunita Gupta Gerlinda HermannDr. Louis D. Curet D. Louis Calongne Associates and T. Daniel *George A. Daniels Associates & deGravelles Delahaye MichaelT. Mr. Eldredge J. *Mrs. Eleanor S.Juliet Dougherty Inc. Endo-Surgery, Ethicon Inc. Engineers, Evans-Graves FerryMs. Stephanie H. and Jack Jr. Field, Sharon Mary Barrett Fruehan General Mills Grady Crawford Construction Inc. Co., Bob Greer Family Gupta/Mittal Alok and Family: Anonymous C. Richard Rogers and Dr. B. Heymsfield and Mrs. Steven Dr. and Mrs. M. Richard Hill Dr. William R. and Marilyn S. Holman IBERIABANK Donald and Kitty Ingram Corporation Invitrogen Anne Rossi JarrettDr. Inc. Management, Craig Jenny Johnson and Mrs. D. William Dr. Gretchen and Lee Kantrow and KrisJane Kirkpatrick Our Donors – Honoring Lifetime Giving Our Lifetime Honoring – Donors

The Pennington The Pennington University System University System 234-acre campus.

the Louisiana State State Louisiana the and conducts basic, basic, and conducts research. More than than More research. Center is a campus of of a campus is Center Biomedical Research Research Biomedical 500 employees occupy clinical and population and population clinical

several buildings on the the on buildings several

FOREWORD

During the two-year span of time covered by this issue of the Scientific Report, we have achieved several significant milestones. Noteworthy accomplishments include the opening of our new clinical research building, construction of the biomedical imaging center, and development of our new strategic plan – Vision 2015.

We are grateful to the Louisiana State University System Board of Supervisors, the Louisiana Board of Regents, and the Commissioner of Higher Education, most recently Dr. Jim Purcell, for the support they have given to our efforts. We are especially grateful for the support given by LSU Board of Supervisors Chairmen, James W. Moore, Jr., Ben W. Mount, and Garret “Hank” Danos, and System President Dr. John V. Lombardi.

In recent times, we have benefitted from the support of our city’s business and political leaders. I would like to thank Louisiana Governor Bobby Jindal, Louisiana Department of Economic Development Secretary Stephen Moret, and Baton Rouge Area Chamber President and CEO Adam Knapp. Baton Rouge Mayor Melvin “Kip” Holden has also taken a keen personal and professional interest in the Center, and we are grateful for his commitment. We also want to express our thanks to John Davies and John Spain of the Baton Rouge Area Foundation for their support over the years.

Our deepest gratitude goes to the men and women who serve on the boards of our two supporting foundations: the Pennington Medical Foundation and the Pennington Biomedical Research Foundation. Mrs. Paula Pennington de la Bretonne, Chair of the Pennington Medical Foundation, and Mr. William Silvia, CEO of the Pennington Medical Foundation, lead a group of dedicated individuals who have made it possible for the Pennington Biomedical Research Center to break ground on new facilities and to acquire sophisticated equipment and technologies on a regular basis. Mr. Tim Barfield, Chairman of the Board, and Mrs. Jennifer Winstead, President and CEO of the Pennington Biomedical Research Foundation, and their fellow board members are fully engaged in the task of raising unrestricted funds and creating endowed chairs and professorships. We are all extremely grateful for their dedication and hard work on our behalf. To all the donors who are so generous in their response to the requests from the Pennington Biomedical Research Foundation, we give our heartfelt gratitude and thanks.

The progress we have made during these past two years would not have been possible without the dedication of our faculty, staff and management team. Their devotion to our research and education efforts makes the Pennington Biomedical Research Center an inspiring place to work.

Our vision is to lead the world in eliminating chronic disease, and our new mission is to discover the triggers of chronic diseases through innovative research that improves human health across the lifespan. We are helping people live Well Beyond the Expected. We have continued to develop the breadth and depth of our science and our ability to achieve our mission. In this report, you will discover a wide spectrum of research programs and projects. You will also receive a brief introduction to the process leading to new discoveries.

To learn more about our science and our Center, as well as about supporting our mission, please visit us online at www.pbrc.edu.

Steven B. Heymsfield, MD Executive Director

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 1 TABLE OF CONTENTS

Foreward………………………………………………… 1 Nutrient Sensing and Adipocyte Signaling A Message from the Executive Director…………………… 4 Laboratory…………………………………………34 Centers and Institutes…………………………………… 8 Nutrition and Neural Signaling Laboratory………………35 What Are Centers of Excellence?………………………… 9 Nutritional Neuroscience and Aging Laboratory…………………………………………36 Center for Research on Botanicals and Oxidative Stress and Disease Laboratory…………………37 Metabolic Syndrome………………………………10 Protein Deficiency and Developmental Nutrition and Obesity Research Center……………………11 Biology……………………………………………38 Center of Biomedical Research Excellence Protein Structural Biology Laboratory……………………39 (COBRE)……………………………………………12 Regulation of Gene Expression Laboratory…………… 40 Institute for Dementia Research and Prevention…………………………………………13 Stem Cell Biology Laboratory……………………………41 Taste Genetics Laboratory…………………………………42 Basic Research………………………… 14 The William Hansel Cancer Prevention Laboratory…………………………………………43 Overview…………………………………………………15 Ubiquitin Biology Laboratory………………………… 44 Adipocyte Biology Laboratory……………………………16 Aging and Neurodegeneration Laboratory………………17 Clinical Research……………………… 46 Antioxidant and Gene Regulation Laboratory……………18 Overview…………………………………………………47 Autonomic Neuroscience Laboratory……………………19 Behavior Technology: Eating Disorders The Blood-Brain Barrier (BBB) Group………………………20 and Obesity…………………………………… 48 Developmental Biology Laboratory………………………21 Behavioral Medicine Laboratory…………………………49 Epigenetics and Nuclear Reprogramming Behavior Modification Clinical Trials………………………50 Laboratory…………………………………………22 Exercise Biology Laboratory………………………………51 Epigenetics and Obesity Laboratory………………………23 Human Physiology Laboratory……………………………52 Gene-Nutrient Interactions Laboratory……………………24 Inactivity Physiology Laboratory…………………………53 Human Genomics Laboratory……………………………25 Ingestive Behavior Laboratory……………………………54 Immunobiology Laboratory………………………………26 John S. McIlhenny Skeletal Muscle Infection and Obesity Laboratory…………………………27 Physiology Laboratory I……………………………55 Inflammation and Neurodegeneration John S. McIlhenny Skeletal Muscle Laboratory…………………………………………28 Physiology Laboratory II……………………………56 John S. McIlhenny Botanical Research Joint Program on Diabetes, Endocrinology, Laboratory…………………………………………29 and Metabolism……………………………………57 Leptin Signaling in the Brain Laboratory…………………30 Pharmacology-Based Clinical Trials………………………58 Mechanisms of Diabetes Complications……………………31 Physical Activity and Ethnic Minority Neurobiology and Nutrition Laboratory…………………32 Health Laboratory…………………………………59 Neurosignaling Laboratory………………………………33 Preventive Medicine Laboratory…………………………60

2 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER Reproductive Endocrinology and Women’s Library and Information Center………………………… 84 Health Laboratory…………………………………61 Mass Spectrometry…………………………………… 84 Sleep Health Center………………………………………62 Outpatient Clinic…………………………………………85 Women’s Health, Eating Behavior, and Proteomics and Metabolomics……………………………86 Smoking Cessation Laboratory……………………63 Recruitment………………………………………………86 Research Kitchen…………………………………………87 Population Science…………………… 64 Transgenics Core…………………………………………87 Overview…………………………………………………65 Chronic Disease Epidemiology Laboratory…………… 66 Contextual Risk Factors Laboratory………………………67 Administration and Finance………… 88 Overview…………………………………………………89 Health Behaviors and Chronic Disease Laboratory…………………………………………68 Computing Services…………………………………… 90 Healthcare Quality Improvement Laboratory……………69 Facilities Management………………………………… 90 Nutritional Epidemiology Laboratory……………………70 Fiscal Operations…………………………………………91 Physical Activity and Obesity Epidemiology Human Resource Management……………………………91 Laboratory…………………………………………71 Intellectual Property, Legal and Walking Behavior Laboratory……………………………72 Regulatory Affairs…………………………………92 Sponsored Projects………………………………………92 Division of Education………………… 74 Overview…………………………………………………74 Adjunct Faculty………………………… 94

Core Services…………………………… 76 Publications…………………………… 98 Overview…………………………………………………77 Journal Articles 2010………………………………………99 Animal Metabolism and Behavior…………………………78 Journal Articles 2011…………………………………… 109 Biostatistics and Data Management………………………78 Books and Chapters 2010……………………………… 120 Cell Biology and Cell Imaging ……………………………79 Books and Chapters 2011……………………………… 121 Cell Culture………………………………………………79 Clinical Chemistry…………………………………………80 Our Supporters………………………… 122 Comparative Biology………………………………………80 Pennington Medical Foundation……………………… 122 Dietary Assessment………………………………………81 A Message from our Foundation Chair………………… 123 Energy Metabolism………………………………………81 Pennington Biomedical Research Foundation Exercise Testing……………………………………………82 Board of Directors……………………………… 124 Genomics…………………………………………………82 A Message from the Chairman of the Board…………… 125 Imaging/MRS……………………………………………83 Chairs and Professorships……………………………… 126 Inpatient Clinic……………………………………………83 Our Donors – Honoring Lifetime Giving……………… 127

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 3 A MESSAGE FROM THE EXECUTIVE DIRECTOR Steven B. Heymsfield, M.D.

IT HAS BEEN ALMOST TWO YEARS since I Excellence (COBRE). In assumed the role of Executive Director at the Pennington addition, we are pleased Biomedical Research Center. Since then we have worked to house the Institute for collectively on developing Vision 2015 – a plan to advance Dementia Research and Pennington Biomedical through three transforming Prevention (IDRP). You strategies, all aimed at discovering the triggers of chronic will read more about disease and improving human health at every stage of these entities later in this report. life. We have introduced a new vision: to lead the world in Economic Impact eliminating chronic diseases and a new mission - to discover the triggers of chronic diseases through innovative research In 2010, the Pennington Biomedical Research Center completed that improves human health across the lifespan. Part of our construction of a new clinical research building. The 90,000 branding effort also includes the slogan or tagline “We square foot building devoted to clinical research stands as a are helping people live Well Beyond the Expected.” testament to our vision of growing our science along three large components: basic research, clinical research and population In this Scientific Report, you will find a description of the basic, science research. The new clinical research facility was the clinical and population research activities as well as the vari- first building on the campus constructed with capital outlay ous other programs of the Center. You will also read about funds from the State of Louisiana. The state’s investment has the core facilities that provide cutting-edge technologies and helped to fulfill the need for additional clinical research space, high quality support to our research enterprise. This report will as our clinical laboratories, staff, and number of participants provide information on the challenges and opportunities and had outgrown the original clinical building. Construction of the the economic impact potential of the Center as we continue to Biomedical Imaging Center, the second building funded by the experience growth in all three program areas. State of Louisiana, began in 2010. This 30,000 square foot building, which is expected to open in the Spring of 2012, will house Organization of the Center advanced imaging systems along with space for support staff As of this writing, the Pennington Biomedical Research Center and other imaging equipment. Pennington Biomedical inves- has about 525 full-time and part-time employees, including 80 tigators will use the new Biomedical Imaging Center resources faculty members and 25 postdoctoral researchers. In addition, to understand evolution of Alzheimer’s disease, the metabolic the Center benefits from the contributions of more than 80 basis of adult-onset diabetes, the CNS mechanisms leading to adjunct faculty members. These scientists are grouped among obesity, and a host of other conditions. These advanced imag- more than 50 laboratories covering basic, clinical, and popula- ing resources will integrate with the Center’s state-of-the art tion science research areas. The research enterprise of the Cen- physiological and metabolic measurement capabilities. ter is also supported by the resources of 19 core facilities. The It is well worth noting that new start-up companies based on administrative organizational structure of the Center has been findings of Center researchers stand to create significant future reorganized to operate more effectively but due to budgetary returns to Pennington Biomedical, the LSU System and the constraints, not all positions are currently filled. state. Among these and perhaps most notably is Esperance The Pennington Biomedical Research Center is home to three Pharmaceuticals, Inc. Investors created this company to take a Centers of Excellence funded by the National Institutes of Health: potent cancer treatment compound from the laboratory bench the Botanical Research Center (BRC), The Nutrition and Obesity to the first human clinical trials. It is significant that when the Research Center (NORC) and the Center of Biological Research FDA reviewed this drug, it was not just examining a new drug

4 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER in an existing category. This compound represents an entirely avenues. In addition, we are exploring ways to self-generate new category of drug which seeks out and destroys cancer cells. new support lines, particularly through opportunities that may Created here by Dr. William Hansel and collaborators, particu- develop in the clinical area. Our team is working with govern- larly Drs. Carola Leuschner and Fred Enright, this new category mental leaders to garner additional institutional support and of drug, if it works as well in humans as in animal models, could spare the Center of further cuts. well save thousands of lives.

Dr. Ken Eilertsen, scientific leader of one of our stem cell research laboratories, created NuPo- tential, LLC, in an effort to capitalize on a new technology for somatic cell reprogramming. This technology may one day allow a physician to treat a patient suffering from damage to an organ (as in type 1 diabetes, Parkinson and the like) by harvesting the patient’s healthy cells from another tissue and “re-programming them” to generate new, healthy cells and tweaking them to assist the dysfunctional organ.

In addition, Dr. Tiffany Stewart received funding from Themelios for the establishment of Body Evolution Technologies, Inc. Body Evolution develops interactive health media based on peer-reviewed research. Treating body image issues can help a wide range of people from obese to thin, including those suffering with eating disorders.

These are just a few examples of scientific ad- Another challenge is that of being able to generate endowed vances that may have considerable impact on medical practice research chairs in the range of $3 to $5 million so that we and health care in the future but may also generate substantial can compete in the national and international market place economic benefits for the state in general and the Center in for the best and brightest scientists. The availability of chairs particular. endowed at these levels will play a key role in the future of the Center. Challenges and Opportunities Competition for the best minds has always been very keen. The most important challenge we face is that of adequate fund- In the case of Pennington Biomedical, there are now sev- ing from the Louisiana Legislature as we continue to feel the eral institutions in the United States that are attempting to impact of budget cuts. The Center’s state general fund budget emulate its success, and a number of these institutions have has been reduced by 19.9% from FY08-09 to FY11-12. During a much larger endowment. We have lost several key faculty this time, new buildings have and are coming online without members during the past several years to other institutions any increase in operating funds to offset basic operating costs. with superior funding. It is therefore imperative that we be in This has resulted in the need to cut funds used to support cer- a position to offer high quality employment opportunities and tain existing programs and we are unable to increase funding generous start-up funds to star faculty candidates, as well as for worthy on-going research. We are working at several levels adequate retention measures to highly successful scientists to secure additional resources for our institution. Obviously, the who have made Pennington Biomedical their scientific home. foundations are continuing their efforts to develop new funding As the governor and legislature scrutinize higher education,

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 5 it is imperative they reflect on the high value return to the throughout their careers. Moreover, they are influential in citizens generated by cutting edge biomedical research. Ad- the world of science as evidenced by the more than 297,000 equate state funding is a critical investment, particularly in an lifetime citations that their research has received to-date in environment where the level of federal funding for biomedical the world scientific literature. scientific research has not increased to any significant extent Our researchers also continue to be recognized in the scientific over the last several years. community for their accomplishments. For example, Dr. Eric Ra- We are in a very strong position as a result of the high quality vussin received the George A. Bray Founders Award at The Obe- of our faculty and a portfolio of research grants and private sity Society’s annual meeting held this past fall; Dr. Kenneth research contracts that is equally as strong. Many of our full Eilertson was named the “2011 University Technology Leader of time scientists at the level of assistant-professor and above the Year” by the Governor’s Louisiana Technology Council; the each bring in more than $500,000 of external research funds American Diabetes Association named Dr. William Cefalu the every year. For example, Drs. Leanne Redman and Corby Editor-in-Chief of Diabetes Care; and Dr. Vishwa Deep Dixit was Martin were awarded a National Institutes of Health UO1 grant chosen by The Gerontological Society of America to receive the to study weight gain during pregnancy in collaboration with 2011 Nathan Shock New Investigator Award. Woman’s Hospital. And a few months ago US Senator Mary Pennington Biomedical is in an excellent position to maintain its Landrieu announced a new competitively awarded $6 mil- leading position and make many contributions because it enjoys lion, three-year grant, from the US Department of Defense to a strong and stable infrastructure combined with strong leader- discover novel ways to increase warfighter resilience, combat ship in each of its major research components. The quality of the readiness, and optimal performance of soldiers. Center’s administrative units and the level of competency and dedi- Pennington Biomedical researchers continue to publish their cation of the employees is another major asset. Moreover, being findings in prestigious scientific journals such as JAMA and able to rely on strong leaders for the BRC, NORC and COBRE center Nature Medicine. Current faculty members have excellent grants as well as for the IDRP initiative bode well for the future. The scientific productivity as shown by the fact that they have quality of the existing facilities and the ongoing expansion of the collectively published approximately 10,000 scientific papers physical plant of the Pennington Biomedical campus constitute

6 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER extraordinary assets that will continue to play a major role in the • Develop a multi-faceted effort to combat the diseases of success of the research enterprise of the Center. aging by offering new approaches to advance healthier aging In addition, the global field of disease prevention research presents a great future filled with exciting opportunities. To take STRATEGY II: Expand Clinical and Public Health Research advantage of these opportunities, our strategy has continued to Efforts to Advance Discoveries that Benefit People in Real be one of investing in cutting-edge basic science areas that will World Healthcare Settings allow us to contribute to the definition of the next generation of • Develop partnerships that translate research discoveries to best practices in public health and preventive medicine. improve patient outcomes Vision 2015 • Expand Pennington Biomedical’s capacity for conducting As the population ages, preserving personal autonomy and a and coordinating clinical trials and longitudinal studies high quality of life has moved into the forefront of the health focused on chronic diseases agenda. It is in preserving a full, healthy life that presents the • Develop an internationally significant pediatric obesity most meaningful opportunity for the Center to make unique research and treatment program contributions. It is evident that one’s genes play a critical role, STRATEGY III: Develop and Harness the Most Progressive, but nutrition and physical activity are also two important deter- Emerging Technology to Facilitate Innovation in Research minants of the rate of decline in overall physical and cognitive independence and in well-being associated with aging. In this • Create one of the country’s first research-focused biomedi- regard, preventing morbidities and remaining free from dis- cal imaging facilities to illuminate the underlying biological ability for a lifetime are of the utmost importance. Prevention processes related to health and disease can and should begin early in life and, as a result, the Center • Translate novel ideas into commercial products and continues to make important investments in developmental services that enhance the health and well-being of people biology, maternal biology and pediatrics. We have a splendid and the scientific and economic vitality of Pennington faculty now probing the mysteries of the body from pre-natal to Biomedical early post-natal through adulthood and to the advanced ages of 80 and 90 plus years. Pennington Biomedical is now uniquely Despite the challenges we face, Pennington Biomedical will qualified to study prevention issues across the lifespan, with continue to grow and make scientific discoveries, recruit out- its broad base in basic, clinical, and population science re- standing faculty, expand our research portfolio, and contribute search and its rapidly expanding efforts in aging and dementia to the economy of the State of Louisiana. research. We will help people live Well Beyond the Expected. Pennington Biomedical will focus its efforts on three trans- formative strategies, all aimed at discovering the triggers of chronic diseases through innovative research that improves human health across the lifespan. Pennington Biomedical will: STRATEGY I: Leverage our Expertise in Nutrition, Physical Activity and Genetics to Enhance Research Programs Aimed at Optimal Aging and Health throughout the Lifespan • Expand Pennington Biomedical’s strong basic science research program in obesity and other chronic diseases to identify and drive novel approaches from theory to practice • Create a personalized health research program that seeks to determine, on an individual basis, a nutrition and exercise prescription to optimize health

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 7 8 CENTERS &INSTITUTES SCIENTIFIC REPORT

2 010–2011 PENNINGTON RESEARCH CENTER BIOMEDICAL CENTERS & INSTITUTES 9 - - 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC developing a better a networks the understanding of developing mol of and functionecules our cells in that manner integrated an in and tissues; enhancing multidisciplinary approaches involving research with complementaryteams expertise better to tackle the complexity asking are questions the and we the research of addressing. problems are disease we

3. 4. on for Botanicals and Metabolic Research The Syndrome Center of Biomedical (COBRE), Excellence Research the Center (BRC), and the Nutrition and Obesity (NORC) the are Center Research Biomedical. They Pennington at of Excellence three NIH Centers theme scientific of the common metabol around organized are three address complementary and all ic disease, components of are of Excellence Centers The Pennington strategy. overall NIH’s expanding to base infrastructure the research and devoted that coursedue in lessen will that providediscoveries will scientific the quality and improve of life. disease the burden of chronic - - -

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON lar events that lead to disease states; disease lead to that events lar building our collective understanding of the precise molecu placing a greater emphasis on mentoring and developing developing and mentoring on emphasis greater a placing facultyour young the discovery who process power now will the future; and in

WHAT ARE WHAT The establishment and support part is of centers of a broader more effectivelystrategy to goal is of the NIH whose overall in people’s improvements into discoveries scientific translate Thehealth. strategy elements of this include: 2. tive effortscenter. tive each within theinvestigators of 1.

Center grants are awarded to institutions with of es groups institutions grants to awarded are Center tablished investigators working in areas of scientific emphasis of Institutes Health. Thedefined all goal of theby National the heart the discovery process at power to is of all NIH Centers scientific advancesby facilitating and enhancingcollabora the CENTERS OF EXCELLENCE? CENTERS Centers of Excellence

CENTER FOR RESEARCH ON BOTANICALS AND METABOLIC SYNDROME

Mission: The mission of the Center for Research on Botani- Our center consists of three cals and Metabolic Syndrome is to pursue an integrated specific research projects, each understanding of the molecular, cellular, and physiological of which evaluates a specific mechanisms by which select botanicals may prevent or re- botanical and assesses the effect verse the development of insulin resistance, the key patho- on pathogenic mechanisms lead- physiologic feature of the metabolic syndrome. ing to the development of insulin resistance. Project 1 investiga- The Botanical Research Center was successfully funded for tors are conducting studies to a second 5-year cycle in 2010 by a grant from the National evaluate mechanisms of action Institutes of Health. Our center is a collaborative effort be- by which selected extracts of tween the Pennington Biomedical Research Center and the William T. Cefalu, M.D. Artemisia sp. modulate insulin Director Biotechnology Center for Agriculture and the Environment at receptor signaling and insulin sen- Rutgers University. sitivity in both animal and early-phase human studies. Project The theme of our center is “Botanicals and Metabolic Syn- 2 investigators are focusing on mechanisms by which selected drome.” The metabolic syndrome has traditionally defined Artemisia sp. extracts and Hypericum perforatum L. (St. John’s a condition whose major features consisted of obesity, insulin wort) affect adipocyte development, adipokines, and insulin resistance, development of type 2 diabetes, and accelerated car- action. Project 3 investigators are evaluating how Asclepias diovascular disease. The development and appearance of other incarnata modulates central mechanisms controlling appetite traditional risk factors, e.g., hypertension, dyslipidemia, and and energy expenditure as a means to improve overall energy nontraditional risk factors, e.g., inflammation, coagulopathy, balance and weight. Our projects are supported by a Botanical are also associated with the condition. Because of the stagger- Core and an Administrative Core. A unique aspect of our center CENTERS & INSTITUTES ing increase in the prevalence of obesity that has now reached is the formation of the Integrative Biology Core, which includes epidemic proportions and the fact that the components of the an Animal Research Subcore and the Analytical Chemistry metabolic syndrome have become increasingly prevalent in Subcore, comprising a clinical chemistry/stable isotope compo- children, this syndrome continues to represent one of the most nent and a proteomic/metabolomic component. This combines important public health problems facing society today. As such, state-of-the-art in vivo metabolic phenotyping with detailed ex the study of botanicals and their effects to modulate pathologic vivo proteomic and metabolomic profiling of serum and tissue processes as part of the metabolic syndrome has become even samples to provide highly integrated metabolic signatures of more important since the inception of our center. the pathophysiology of insulin resistance and its resolution by botanical extracts. As such, each project is supported by Goals cutting-edge technologies that include metabolomic profiling, proteomic assessments, and bioaccessibility determinations. The scientific goal of our center is to provide a comprehensive evaluation of specific, compelling hypotheses about the Collaborative institutions and faculty within the Center for molecular, cellular, and physiological mechanisms by which Research on Botanicals and Metabolic Syndrome include: botanicals can modulate the development of the underlying Pennington Biomedical Research Center: William T. pathophysiologic mechanisms of, and attenuate the develop- Cefalu, M.D.; Zhong Q. Wang, M.D.; Jacqueline Stephens, Ph.D.; ment to, metabolic syndrome. To accomplish our goal, our Thomas Gettys, Ph.D.; Jennifer Rood, Ph.D.; Randall Mynatt, center has encompassed the disciplines of nutrition, plant Ph.D.; Phillip Brantley, Ph.D.; William Johnson, Ph.D.; Indu Khet- chemistry/characterization, metabolism, physiology, endocri- erpal, Ph.D. nology, molecular and cellular biology, and genetics and has Biotech Center, Rutgers University, New Brunswick, NJ: spanned both the basic and clinical sciences. Thus, our interdis- Ilya Raskin, Ph.D.; David Ribnicky, Ph.D. ciplinary approach has allowed for a comprehensive evaluation of botanicals on pathogenic processes by evaluating multiple North Carolina State University Plants for Human cellular mechanisms of action. Health Institute: Slavko Komarnytsky, Ph.D.

10 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER Centers of Excellence NUTRITION AND OBESITY RESEARCH CENTER

Mission: The mission of the Nutrition and Obesity Research the entire age span, from gesta- Center (NORC) is to facilitate and promote collaborative and tional and perinatal development multidisciplinary interactions that will foster new research through childhood and adoles- ideas and enhance the translation of basic nutritional cence, to young and middle-aged research findings into the clinical arena and ultimately into adults up to elderly individuals. practical application. The resources of the NORC assist The NORC has chosen “Nutritional Programming: Environ- investigators at the center and mental and Molecular Interactions” as a central focus to Louisiana State University (LSU) in

develop. This focus is based upon convincing basic science and Baton Rouge and at the LSU Health CENTERS & INSTITUTES clinical data indicating that an interaction between genes and early Sciences Center in New Orleans to Eric Ravussin, Ph.D. Director life environmental conditions is important in the development of address the pathophysiology and obesity and the different facets of the metabolic syndrome. Our molecular mechanisms leading to aim is to focus the NORC’s efforts around this emerging and impor- different facets of the metabolic syndrome. These research pro- tant theme. By keeping this tight focus and avoiding too broad an grams are conducted to instigate the effects of gender, racial, and approach, the NORC is more likely to produce meaningful syner- ethnic background within a context of cultural factors. gies and expansion of research efforts including basic, clinical, and The NORC is bringing to the established research base at Pennington population science. Biomedical a structured system to provide core services to: The National Institutes of Health (NIH)-funded research base on (a) support the research base, and which the NORC has been established includes basic and clinical (b) promote novel investigation around our chosen theme of research addressing the most prominent causes of morbidity and molecular mechanisms of nutritional programming induced by mortality in the United States related to nutritionally induced environmental factors. chronic diseases, many of them linked to obesity. The NORC’s platform includes three scientific cores: a Molecular Mechanisms The NORC provides a mechanism to enable both Pennington Core (genomics and cellular imaging), very closely related to Biomedical and the NIH to maximize the effect of research funding. a Human Phenotyping Core (characterization of phenotypes As of November 2011, there were more than 120 members of the predisposing to obesity and the metabolic syndrome and behav- NORC, including people outside of Pennington Biomedical. One ioral interventions to counteract those), and an Animal Models of the most successful programs provided by this Center Grant is and Phenotyping Core (generation of genetically engineered the yearly funding of Pilot and Feasibility grants to implement new animal models and a battery of instruments to phenotype them). innovative research around our theme of nutritional programming. Furthermore, the NORC supports clinical investigation address- Such studies allow young investigators to collect adequate prelimi- ing the etiology of nutritionally induced chronic diseases across nary data as the basis for larger NIH research grants. Since the start of the NORC in 2005, a total of 34 Pilot and Feasibility grants have been awarded from our funding (approximately $20,000-30,000/ each award). From these projects, 15 grants have been submitted and 7 have been funded (5 NIH, 2 American Diabetes Association). For more information on the NORC, visit http://norc.pbrc.edu/

The mouse T-maze was developed at the Pennington NORC from the Stone T-maze for detection of learning and memory impairments in mouse models. It is unique in requiring the mice to wade through water to “escape” to a dry, dark goal box, which minimizes noncompliance since the mice are motivated to get out of the water. At the same time, since mice are wading rather than swimming, it can be successfully completed by old or motor-impaired mice. It also measures learning without use of food rewards, providing a clear measure of learning independent of differences in food-based motivation that may be characteristic of certain mouse models of obesity.

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 11 12 CENTERS & INSTITUTES critical to regulation of energy homeostasis andtheassociated to critical homeostasis regulation ofenergy questions fundamental is pursuing each project techniques, Using in vivo drial in biogenesis adipocytes. in obesity, programming toepigenetic regulation ofmitochon sis, tomechanisms inflammatory linked adipogenesis, to mechanismsneural ofglucose homeosta sensing andenergy translational from to ranging questions address approaches cellular, of combination a employing are who molecular, and faculty junior outstanding from fiveprojects COBRE supports independence by Pennington Biomedical investigators. The mentored andpromising totors will be who young scientists disease accomplished bybolic recruiting investiga senior to enhance COBRE researchmeta The ison leading an effort to as “metabolicsyndrome.” referred collectively of pathologies anddiabetes are central ofacluster elements Obesity diabetes. has and8%ofthepopulation overweight obese, adult-onset or is population clinically either oftheU.S. two-thirds Nearly disease. mechanismsthe underlying ofmetabolic to make that discoveries is fundamental technology needed of and (2) thecutting-edge with providingouryoung scientists will power who process,ing theyoungdiscovery scientists ment that places emphasis strong on mentoring and develop (1) acollaborative fostering research andinteractive environ PenningtonThe COBRE is Biomedical accomplishing by goal its BIOMEDICAL RESEARCHEXCELLENCE CENTER OF metabolic disease. ofthecellular mechanisms andmolecular ofunderstanding mass in Louisiana our ofscientists advancing on focused Researchcal Excellence (COBRE) thecritical is to expand Mission: SCIENTIFIC REPORT The mission The ofthePennington Center ofBiomedi Key COBRE personnel, April 2011. April Key COBRE personnel, From Salbaum, Thomas Gettys, David Burk, Burk, David Gettys, Thomas Salbaum, left to right, Drs. Zhanguo Gao, Ji Suk Ji Gao, Zhanguo toDrs. right, left David McDougal, and Greg Sutton. Greg and McDougal, David Chang, Heike Muenzberg, Michael

2 010–2011 , in vitro vivo vitro in ex and ------Institutes Health. of theCenterfrom National Research of Resources the at National PenningtonThe COBRE Biomedical by agrant is supported http://cobre.pbrc.edu/ disease. ofmetabolic problem national to ofsolutions thediscovery health theexpanding port ourmany upon build successes ongoing as we continue to sup five isyears to next five goal during the Our of the years COBRE. that we have duringthefirst research infrastructure developed outstanding the of (d) and utilization research, enhancing institutionalwith in colleagues clinical/translational engaged collaborative for interactions newopportunities and fostering ofCenter strengths existing ments investigators, (c) developing disease research that in metabolic comple engaged faculty pendent funding, (b) recruiting junior outstanding and senior inde to sustainable them mentoring and institution the within from junior faculty outstanding (a) developing and retaining by obesity/diabetesin research engaged investigators productive mass the critical of expand five second are further years to COBREnington in its Biomedical aims of the specific Pen The ment ofobesity. develop the tissue massduring linked to ofadipose theexpansion disease ofmetabolic pathologies PENNINGTON RESEARCH CENTER BIOMEDICAL - - Director Ph.D. Thomas Gettys, - - - INSTITUTE FOR DEMENTIA RESEARCH AND PREVENTION

Focus: This Institute is currently involved in a wide range of of these research efforts or who studies aimed at understanding the causes of brain aging and would like to participate are identifying the basis by which aging promotes the develop- encouraged to contact the IDRP ment of dementia, focusing on lifestyle choices as modulators at 1-877-276-8306 or to e-mail of cognition. Secondary emphasis is understanding the basis [email protected] for more for frailty and falls in elderly with and without dementia. details.

A central focus of the Institute for Dementia Research and The IDRP is supported by Prevention (IDRP) is obtaining information from our grow- private philanthropy as well as a

ing registry of over 1,600 individuals from Louisiana, aged combination of National Insti- CENTERS & INSTITUTES 60 and over, who receive annual neurocognitive exams and tutes of Health, private founda- Jeffrey Keller, Ph.D. Professor a host of other evaluations focused on understanding the tion grants, and pharmaceutical Capital One/Edward basis of brain aging and dementia. Studies use cutting-edge industry sponsored studies. G. Schlieder Chair analysis in laboratory and free-living settings and will soon be incorporating neuroimaging. An increasing number of Pennington Biomedical faculty and faculty from institutions Director: Jeffrey Keller, Ph.D. around the country are working with the IDRP to increase Faculty: Timothy Church, M.D., M.P.H., Ph.D.; William the impact of its efforts. This is accomplished by using our Gahan, M.D. (Adjunct); Corby Martin, Ph.D., Valerie registry and existing data to secure more grants and pub- Myers, Ph.D.; Catrine Tudor-Locke, Ph.D. lications in multiple areas of geriatric research. In addition to these efforts, the IDRP conducts a variety of clinical trials Research Team: Robert Brouillette, M.S., Heather focused on dementia treatment, participating in major Foil, B.S., Ciara Foster, B.S., Leslie Guillory, B.S., pharmaceutical industry-sponsored trials as well as lifestyle Danielle Nye, B.A., Allie Piehet, B.S., Sandy Hyatt interventions. Those interested in learning more about any

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 13 BASIC RESEARCH

14 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER At Pennington Biomedical Research Center, Basic Science con- Our faculty in Basic Science have made important findings in tinues to be a thriving and world-class research program. At the multiple areas of research, and these findings will have a signifi- heart of the Basic Science research efforts is a focus on under- cant impact on the future of medicine and health. Work in Basic standing how metabolic dysfunction contributes to the genesis Science at Pennington Biomedical has identified the triggers and progression of the chronic diseases that affect us today. responsible for regulating hunger, increased our understanding It is now clear that the metabolic dysfunction associated with of the mechanisms by which dietary fat and obesity mediate conditions such as obesity and diabetes is intimately involved disease, and helped to uncover in many of the worst diseases of the 21st century including the process by which com- cardiovascular disease, immune disorders, cancer, and demen- munication between the brain tia. Given the long-standing history of the Center as a leader and peripheral tissues occurs. in metabolism research, we are perfectly suited to increase the Importantly, efforts in Basic

understanding of the interconnected nature of chronic diseases. Science have also identified BASIC RESEARCH The theme of metabolic dysfunction is the thread that unites the basis for complications of the diverse expertise and scientific interests in the Basic Science metabolic disorders such as program, and it is one of the major factors distinguishing the diabetes, providing insight as to work at Pennington Biomedical. the origins of diabetic neuropathy and diabetic nephropathy. The Basic Science program at Pennington Biomedical has add- Jeffrey N. Keller, Ph.D. Studies in these areas are of the Professor ed several key faculty to strengthen our repertoire in the areas highest impact because of the Capital One/Edward G. of oxidative stress, neuroscience, cell signaling, and physiology. Basic Science core laboratories, Schlieder Chair The addition of these faculty not only brings new state-of-the- which provide our faculty with Associate Executive Director for Basic art and cutting-edge research programs to the Center, but also the most cutting-edge genom- Research allows us to leverage this expertise with our existing scientists ics, proteomics, transgenic to increase the impact of ongoing research. Coupled with animal, animal behavior and continued investment by Pennington Biomedical into the Basic phenotyping, and cell bioim- Science core facilities, the Pennington Biomedical Basic Science aging technologies available. program is well poised to continue to build off of our suc- These findings are vital in cesses. And we have had multiple successes, including the fact moving from “bench to bedside,” taking our findings from the that since the last reporting period we have renewed our Na- laboratory to the clinic in order to develop new and important tional Institutes of Health (NIH) grants for the Center of Biomed- treatments for chronic diseases. ical Research Excellence (COBRE) and the Center for Research on Botanicals and Metabolic Syndrome, providing another 5 years The landscape of Basic Science research continues to change of vital support. The ability to renew these center grants during and evolve. With the tightening of NIH budgets and constant a period of intense competition for NIH dollars is a tremendous improvements in science and technology, our researchers must testament to the Basic Science researchers involved in each of continue to be the best, brightest, and most equipped in order these large programs. Our scientists continue to be prolific in to succeed. Our scientists continue to secure competitive fund- their publications (over 200 peer-reviewed publications since ing at every level, ranging from independent investigator grants the last report), with the highest tier of journals represented. to large center grants. And while the environment will remain This includes a publication by multiple Pennington Biomedi- challenging, we believe that we will continue to be successful. cal scientists in Nature (led by Dr. Vishwa Deep Dixit’s research This assurance comes from our long track record of success, the team). Our Basic Science faculty have demonstrated their commitment of Pennington Biomedical to continuing to grow a innovation by generating multiple patent applications and suc- strong Basic Science program, the collaborative spirit of our cessful business grants, with Dr. Kenneth J. Eilertsen receiving faculty, and the unique resources and expertise that allow us to the University Technology Leader of the Year Award from the delineate the role of metabolic dysfunction as a trigger for the Louisiana Technology Council. diseases of today.

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 15 16

BASIC RESEARCH Adipocyte BiologyLaboratory Professor Jackie Stephens, Ph.D. to We regulate expression. gene have andproteinsecules that to bind can DNA the nucleus. STATs are signalingboth mol STATs activated, When factors. move into andgrowth ofhormones by avariety which are activated factors, transcription we have theSTAT studied oflatent family particular, In cellular processes. important lators ofdevelopment andseveral other regu thatmaster are factors transcription sion. Hence, on ourresearch has focused expres gene maintenance ofadipocyte and induction involved are the in and that regulate thedifferentiationfat factors scription of cells significantly tranadvanced identification by of hasthe been contribute to disease ofhow adipocytes understanding The syndrome. metabolic diseases including and obesity,genesis T2DM, ofmetabolic level and determines how these cells contribute to the patho cells offat at themolecular basic functions studies Laboratory Biology Adipocyte The adipocytes. from secretion hormone of themechanisms that control lipid storage, insulin and action, related result in disorders dysregulation andits tension. Obesity 2diabetes (T2DM), cardiovascular disease,of type andhyper disease cells offat thedevelopment for andamajor risk factor is theprimary body. in thewhole Obesity homeostasis energy sitive cells that make contribute hormones to theregulation of insulin-sen These organisms. vertebrate in homeostasis energy arecells highly specialized that play amajor in role Adipocytes molecular pathogenesis of type 2diabetes. pathogenesis oftype molecular cellular pathways involved the in to understand approaches FOCUS Hormones in circulation bind cell surface receptors SCIENTIFIC REPORT and result in the activation of STATs, of activation the in result and are which signaling molecules that can move into the into move can that molecules signaling : The goal of this laboratory is to ofthis use goal biochemical The laboratory nucleus to control gene expression. gene to control nucleus

2 010–2011 - - -

- - - - and Metabolicand Syndrome. Center forResearchby the from funds NIH-supported Botanicals on Research by grants this and in the isfrom NIH supported laboratory diseases. metabolic by individuals affected in that botanicals could have toeffects identify hope beneficial by undergraduates. LSU haveies We conducted largelybeen stud These function. thatbotanicals regulate can adipocyte Syndrome at Penningtonand Metabolic to identify Biomedical We theCenter with are also working Research Botanicals for on the National Institutes ofHealth (NIH). in 1996 by andis started funded This project andT2DM. obesity and may contribute underlying to the understanding defects homeostasis energy regulating mechanisms into molecular the leadto insights will hopefully animal. studies in thewhole Our cells fat from andmodulatesecreted overall insulin sensitivity revealed that STAT5 also ofproteins can control theexpression have recent experiments most Our synthesis andinsulin action. have shown how STAT5 regulates associated lipid with genes development in mammals. Inmature cells, fat weto adipocyte recently shown that proteins, ofthese one STAT5A, contributes Zhao, B.S.; Ursula A. White, Ph.D. White, A. Ursula Zhao, B.S.; Peng B.S.; Boudreau, Anik Ph.D; J. Richard, Allison Team:Research Jackie Stephens, Ph.D. Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL - BASIC RESEARCH 17 - - - - - 010–2011 2

There are many modulators of cognitive decline these how understanding and aging, during factors work together delay to or promote cognitive decline and dementia a major is focus laboratory. Keller the of SCIENTIFIC REPORT SCIENTIFIC Sleep Gait Stress Activity Physical Faculty: Ph.DJeffrey Keller, Research Team: Kalavathi Dasuri Linnea Ph.D, Freeman Ok Ph.D, Sun Fernadez-Kim B.S., Le Zhang Ph.D tion) to understand to function cognitive a how across altered tion) is spectrum a variety use We of experimen and disease. aging of tal models, molecular studies, study to and neuroimaging this important These of research. area efforts only not will imporbe understanding in tant the role of diet and obesity regulating in interventions novel to lead brain pathogenesis, may but also of age-related Alzheimer’s for as the treatment such dementias disease. These efforts science basic theby Institute complemented are founded and (IDRP), for and Prevention Dementia Research The IDRPconducts Jeffrey Keller. a directed longitudinal Dr. by 60 Louisiana, study in aging over 1,800 individuals more in than examining the causes of dementia and increased falls in the identification the tests for new developing as well as elderly, study This of early dementia. comple is aging of longitudinal conducts also Dr Keller the JoLamar mentia, Dementia Study. a variety based exercise including for dementia trials of clinical directing as for well Alzheimer’s as trials trials, clinical clinical companies. pharmaceutical with major disease laboratory supported NIH is from the in this grants by Research and NIDDK, NIA the by Lamar from the and Family, philanthropy and Foundation, benefactors. other Coypu pathology. In addition, our studies use classic and innovative and innovative In addition, our studies classic use pathology. func motor memory, (learning, behavior of animal analysis a longitudinal study by with activemented individuals in de - - - Body Fitness COGNITION Composition Lifestyle Nutrition This laboratory is currently involved in a wide in range laboratoryThis involved currently is :

Depression

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS of studies aimed understanding at causes the of brain ag ing and identifying the promotes aging which by the basis of brain pathologydevelopment as such and brain diseases Alzheimer’s disease. Our studies look a variety at of pathologies oxida including taneous adipose (tissue under the skin). Our current National Our National current under skin). the taneous adipose (tissue (NIH)-funded of Health Institutes studies focus on the role of oxidative stress in mediating neuropathology and dementia, as directly as well looking in adipose the role of visceral at tissue mediating specificaspects cognitive and of brain pathogenesis decline. tive stress, inflammation, vascular and Alzheimer’s pathology, The studies our laboratory in look the molecular of at basis on brain focusing the effects aging, obesitydiet-induced of promotes adipose which by tissue and the mechanisms (DIO) understand designed are to Studies brain and pathology. aging metabolicwhat disorders or factors from are adipose tissue Our dementia. and neuropathology mediating for responsible studies build upon published previously that work showing adipose) of the abdomen withfat organs associated (visceral brain mediating in dysfunction more involved is subcu than JeffreyPh.D. Keller, N. One/Edward Capital G. Schlieder Chair, Professor, ExecutiveAssociate Director for Research Basic Aging and Neurodegeneration Laboratory Neurodegeneration and Aging 18

BASIC RESEARCH Antioxidant Regulation Laboratory andGene Professor Ye,Jianping M.D. Inflammation stimulates angiogenesis in adipose tissue during adipose in stimulates angiogenesis Inflammation that: in thebody. (a) suggest homeostasis studies energy Our tissueplays andmaintenance in adipose arole remodeling of in obesity.largely unknown Itis inflammation not clear whether of inflammationthe literature,remain activities thebeneficial of inflammation beendocumentedwellhave tivein effects andPPARγ. such as C/EBPs, tion factors nega Although these mechanism molecular involvesThe suppression oftranscrip expression. andsuppressiontion oflipolysis, ofadiponectin stimula degeneration, ofadipocyte induction of adipogenesis, cellular The acids. mechanism is related to inhibitionand fatty inflammationin glucose disorders metabolic is with associated sitetissue ofchronic inflammation is aprimary obesity.in The liver, Adipose atherosclerosis. and fatty syndrome, metabolic 2diabetes,genesis ofmany chronic diseases including type patho the to inflammation contributes Obesity-associated several laboratories including ours. several laboratories including ours. mation in thebody, which have uncovered in been by studies believe that of inflam this is duetoactivities thebeneficial theconcept. evidence in clinicalsolid We trials to support provide to able havebeen not approaches anti-inflammation insulin However, of sensitivity. pathogenesis the in activities detrimentalinflammation its for wellbeen documented has regulate insulin inflammation resistance? Obesity-associated (1) inflammation Whyoccur does obesity?in (2)Howdoes FOCUS SCIENTIFIC REPORT : Our research is conducted to address two questions: to research two address Our is conducted Assistant Professor Assistant Zhangou Gao, Ph.D.

2 010–2011 - - - - - tional Institutestional the American HealthAssociation. and of Diabetes Research by grants this in the isfrom Na supported laboratory we believe that thedevelopment for is it ofobesity. required obesity. We call this condition “inflammation resistance,” and leading toto risk expenditure, an increased promoteof energy able be not will inflammation the inflammation, of this activity to theresponse loses thebody If metabolism. tion ofenergy regula the in role playsimportant an inflammation associated in thebody. have results that These us to obesity- led propose that prevents accumulation fat expenditure increase in energy mechanism The is diet. related ahigh-fat to fed antance when andinsulin resis linesboth oftransgenic miceobesity from obesity. Chronic inflammationprotects models dietary in these of models mouse oftwo studies phenotype sion from is derived tissue. adipose by produced This conclu cytokines flammatory and mediated is by in impact broad has a activity inflammatory in tissue thisremodeling, effect local from diture.the Different by expen stimulationnance ofenergy homeostasis ofenergy inflammation activity, In thesecond contributes to the mainte gators field. in the obesity acceptedhypoxia has been by an increasing ofinvesti number is concept enhanced.tissue The ofadipose factors of angiogenic infiltration Macrophage elevated,expressionis and adipocytes. in apoptosis and of lipolysis induction inflammation by chronic toresponse thehypoxia. Additionally, hypoxia contributes to such as HIF-1α factors oftranscription activation in andNF-kB tissue after adipose in induced inflammation is tissue. The pressure in adipose in oxygen leads to areduction that obesity tissue We remodeling. ofcompensatory the process reported signals theangiogenic tissue in in adipose response to amplify inflammationhypoxiafrom derived a is effect, In thefirst surplus in obesity.energy to responses the feedback thebody’s represent activities two in themaintenance These expenditure homeostasis. ofenergy (b) and energy tissue enhances expansion; quick inflammation M.S.; and Hongyun Lu, Ph.D. Hongyun and M.S.; Zhao, Zhiyun B.S.; Jing, Xiaoting M.S.; Hao, Zheng Yong Ph.D.; Ye, Xin Lee, Han Ph.D.; Zhang, Jong B.S.; Team:Research Ph.D. Ye, Gao, Jianping Zhangou M.D.; Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL ------BASIC RESEARCH 19 ------010–2011 2

Faculty: Richard Rogers, Ph.D.; Gerlinda Barnes, Maria Ph.D.; Hermann, Ph.D.; David McDougal, Ph.D. Research Team: Eduord Viard, Ph.D.; Tina H.T. VanMeter, Live cells recorded from cells Live a brainstem identifi the showing preparation slice cation of glia and (astrocytes) neurons plus the effect of a thrombin-like pep tide (SFLLRN) to activate glial cells. (A) activate to glial cells. (A) tide (SFLLRN) intracellular tracks dye green Calcium calcium changes in both neurons and glia, while the vital stain sulforhoda (C) astrocytes. only stains (B) mine discrimination shows image Merged between astrocytes and neurons. illustrates the effect(D) to SFLLRN of neurons. and astrocytes both activate neurons first; activated Astrocytes are neurons Astrocytes activate follow. through the release of glutamate. SCIENTIFIC REPORT SCIENTIFIC David McDougal, Ph.D. David Instructor tion. This discoverytion. supports This neurons and cells glial the idea that form a complex functional network bodily regulates functions. that laboratory supported NIH. is from the in this grants by Research rameters also regulate the sensitivity of glial cells to chemical stimula to the sensitivityrameters regulate cells also of glial evoke changes in cellular activity. These of the brainstem cellular in areas changes evoke outsideare the blood-brain barrier and therefore can be affected the brain generated or the periphery either within in thrombin by found thrombinin response that We and thrombin- injury. to peptides produce applied the brainstem gastriclike stasis. to effect this by a the brainstem was mediated within Surprisingly, have interaction betweennovel cells and neurons. Glial cells glial long supporters been passive of as thought the cells of neurons, responsible for long-distance electrical By using communications. foundlive-cell we methods laboratory, imaging developed this in thrombinthat first activates in cells the glial brainstemby causing re to cells the release of stored turn, in causes calcium. This, glial onto transmitter normally a neuronal substance, lease glutamate, the reflex in the of regulation stomach. involved neurons adjacent caus activated, then are neurons eventually These gastric-control of gastric motility. ing a shutdown The cell-mediated reflex discoveryis of a glial autonomic a be very important may that one phenomenon, new completely the generalin understanding the brain detects of how and reacts injury following chemical agents cells or released theto other by onset of disease. fibers direct afferent vagal that determined that have In addition, we based control physiological on pa in changes autonomic in changes - - - Maria Barnes, Ph.D. Assistant Professor Gerlinda Hermann, Ph.D. Associate Professor This laboratory This determin in generally interested is :

the basic neural circuitry involved in the control of digestive neural circuitrythe basic the control in involved processes, activated infec cells during immune which by the mechanism tion, cancer, radiation, or autoimmune disease cause autonomic disease or autoimmune radiation, cancer, tion, fail, to of the stomach regulation detect the brainstem in cells glial which by the mechanisms responsible for neurons turn and in regulate chemical signals control, autonomic of metabolic responsible forthe regulation the mechanism heat production the by brainstem, and chemosensory functions nerve cranial of afferents.

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS • • ing how the brainstem controls autonomic functions autonomic to controls related brainstem the ing how metabolic homeostasis. behavioral and • • • tion between autonomic in changes in resulting and neurons glia a function as control of disease. endocrinologist and the famousEighty physician ago, years observedHarvey bleeding Cushing that closed often head injuries produced gastric ulcers severe caused a complete primarily by theory original His motility. that was of gastrointestinal suppression increases pressure intracranial caused in head trauma by directly activated parts in involved of the brainstem shown of digestion. the regulation later It was whilethat intracranial pressure does not control failed autonomic with well correlate general in does. trauma of the stomach, That bleeding severe is, caused transplant by trauma, and burns accident severe surgery, with failed correlated autonomic highly all are of the stomach. control suspected a critical of component We that the blood-clotting cascade, thrombin, might be responsible for the connection between The portion failure. autonomic and trauma responsible forof the brainstem autonomic concentration a high of the gut has control can act thrombin which through of sites to Present activities include investigations of: activitiesPresent investigations include This work has been has work This supported grants five by thefrom National Some publications of our more recent (NIH). of Health Institutes communica neuroscience: with new to a phenomenondeal is that Richard Rogers, Ph.D. Professor Autonomic Neuroscience Laboratory Neuroscience Autonomic 20

BASIC RESEARCH The Blood-Brain (BBB)Group Barrier Harris J. Chustz Chair Chustz J. Harris Professor, Companies/ United M.D. J. Kastin, Abba mation, and autoimmune diseases. anotherin obesity, component oftheBBB, neuroinflam cytes, Lately,substances. we have ofastro focusingtherole on been harmful from brain the protecting while functions regulatory in engaged that andbrain is actively thebody between interface lies in theconcept studies of these that is adynamic theBBB cerebral endothelial cells composing the significance The BBB. also in the but elicit crosssignalingonly theBBB, transduction volved. Inrecent years, we showed that small these proteins not in mechanisms the way describing the in leading we still are and have (CNS) system central nervous effects, in theperiphery theconcept ago,Several wethat decades pioneered peptides 2. 1. in feeding behavior.in feeding involved to related peptides/polypeptides particularly trol, in neuroendocrinethe con endothelia BBB and astrocytes lism by neuroinflammation. andmetabo flow well as theregulation ofcerebral blood by way as oftheBBB, functions late system central nervous FOCUS

lational regulation in astrocytes. astrocytes. in regulation lational intracellular signaling mechanisms thetrans for responsible and and cellular assays factors to determine themetabolic tive astrogliosis? studies transport We BBB are conducting as a result of reac nonspecific or selective leptin system astrocytic the in receptors? changes leptin the Are astrocytic andother challenges to theCNS upregulate obesity How do miceleptin receptor in generated ourlab. knockout ofastrocyte-specific thephenotype tomethods characterize electrophysiological, immunohistological, and biochemical use behavioral, in vivo studies Our activities. astrocytic havemodulates signaling that leptin challenges shown hypoxic and excitotoxic Inaddition, of gliotransmitters. determineproduction sionmarkers and the of inflammatory leptin receptor, ourcell theexpres test culture approaches cephalomyelitis. To oftheastrocytic elucidate thefunctions diet-induced and autoimmune experimental obesity en mice in with is upregulated receptor leptin that the shown We have functions. ofleptin signalingRole astrocyte on SCIENTIFIC REPORT responses to scratch injury in culture, which is further modulated by metabolic by metabolic modulated is further which culture, in to scratchresponses injury : factors from the serum of mice with diet-induced obesity (DIO) and by (DIO) leptin. and obesity diet-induced mice with of serum the from factors Astrocytes (green) and microglia (red) show different reactive and migratory migratory and reactive (red) (green) different show microglia and Astrocytes This laboratory investigates laboratory This Professor Ph.D. M.D., Pan, Weihong

Italso investigates of therole 2 010–2011

how cytokines modu cytokines how Cancer Institute, Jiaotong University Shanghai from Professor Visiting Tu, Ph.D. Hong M.D., ------4. 3. Institutes Health. of by the National is supported Research the in BBB Group 5.

receptor knockout micereceptor in generated ourlab. knockout autoimmune encephalomyelitis in endothelial-specific leptin experimental determine or diet theoutcome of ahigh-fat andtheautoimmune response? ofobesity opment We will What is ofendothelial therole leptin signaling in thedevel leptin receptorknockout mice.and the astrocyte-specific inhibitors activity astrocyte achieved by theuse ofselective andcognition. This metabolism, will be including feeding, tor neuronal on leptin signaling andbehavioral outcome, leptin recep or the astrocytic activity of astrocytic Effects nals theperiphery. from sig neuroinflammatory in theeventthe BBB of signaling by modification of principle important an illustrates This challenge. IL15 to in response aTNF andfate of determine thecompartmentalization further of cerebral IL15 signaling. will studies Cellular trafficking regulator ofIL15 in endothelia and afacilitator production endothelia?bral We have positive shown that is astrong TNF modulateIL15 TNF endogenous How does incere trafficking PENNINGTON RESEARCH CENTER BIOMEDICAL Instructor P.Kirsten Ph.D. Stone, - M.S. Ph.D.;Jayaram, Yuping Wang, Bhavaani Ph.D.; Mishra, Pramod M.S.; Hsuchou, Hung Team:Research Ph.D. P. Kirsten Ph.D.; M.D., Stone, Tu, Hong Ph.D.; M.D., Pan, Weihong M.D.; J. Kastin, Abba Faculty: - - - BASIC RESEARCH 21 - - in 010–2011 2

Birth Defects Research SCIENTIFIC REPORT SCIENTIFIC processes, such as neural tube closure and development of the of the and development processes, neural tube as closure such identified en the visceral In the past have we 2 years, skeleton. that shows evidence Emerging and disease. environment, tional for long-lasting consequences has development intrauterine adverse that exposures such of the individual, raise the health metabolic including life, in syndrome later for disease the risk The knowledgeand obesity. gained from our studies help will diet during pregnancy birth maternal prevent optimize to to defects health. long-term promote and doderm as the major site of expression for folate receptor 1, the the 1, of expression receptor doderm for site folate major the as primary embryo the developing transporter to prior of folate to G. the James awarded was work This Wilson neural tube closure. Society for the Best of the Teratology Paper Award Publication published the scientific journal in identifiedto folate; respond recently genes that also We 2009. energy to metabolism. provide they a link intriguingly, dietary respondGenes to that the placenta in factors as such identifiedlab. in recently our also were content fat and protein the in placenta These inflammation genes there is suggest that an by be aggravated may this diabeticin pregnancies, and that influences diet also adverse diet. The of maternal composition for neural tubethe risk defects stages earlier at development, in between a complexindicating interplay gene expression, nutri Faculty: Kappen,Claudia Ph.D.; T. Michael J. Salbaum, Ph.D. Research Team: JacieMacGowan; Xiaoying Zhang,M.D.; Claudia Kruger,Ph.D. ------J. MichaelJ. Salbaum, Ph.D. Associate Professor The laboratory goal understand of this to is ma how :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS ternal factors, ternal nutrition during pregnancy, as or diseases such encoded the genome information realized in shape how is a three-dimensionalinto living during organism develop ment. bryos known be is critical to exposed signaling diabetes. Wnt to embryogenesis under its but in role development, normal for unknown. is diabetes There emerging is of maternal conditions nutrition and metabolism, to signaling links that evidence Wnt obesity. as such conditions pathological and normal under both established also Therefore, we a mouse model for obesity dur Our efforts genes demonstratedthat the in yearshave past 2 em in misregulated are pathways signaling belonging Wnt to Neural tube defects rich in is a diet that by can be prevented leafy present in vitamin. acid is Folic essential an folic acid, green vegetables and folate-fortified products;cereal and grain folic acid. Despite multivitamin supplements contain also many underlyingits the mechanisms the beneficial wide use, effectof poorly supplementation are folate understood. In fact, re it has man development and are well-suited to identify to well-suited and are development man critical dietary factors interacting as genes. well as known be also is a strong to which factoring pregnancy, risk for neural tube defects. but that a panacea, not is folate been that mice cently in shown neural tube promote defects,it can even the need highlighting mouse investigate models using to are for more We research. the effects supplementationkey morphogenetic on of folate pass both gene-gene and gene-environment interactions, but gene-gene both but pass gene-environment interactions, and been these in latter interactions have of the genesfew involved Our model date. to identifiedusessystem diabetic pregnancies a well-known is diabetes causing the mousein since teratogen, primarily heart and neural tube defects. pregnancies In humans, a higher by accompanied are diabetes maternal by complicated for defects, congenital risk with heart defects and neural tube defects particularly infants in exposed common maternal to diabetes. Therefore, mouse our models reflect hu processesin It is generally believed that developmental programs developmental encom generally believed that It is Claudia T. Kappen, Claudia T. Ph.D. Professor Developmental Biology Laboratory Biology Developmental 22

BASIC RESEARCH Epigenetics andNuclearReprogramming Laboratory Associate Professor Associate Ph D. J. Eilertsen, Kenneth used cellsconditions raregenetic with used patients such as from success first The usingiPS cells a disease study ina to dishPetri of drugs. clinicalentities, trials” andto to develop thousands test “virtual new drug identify to side effects, possible for drugs to screen what is apatient’s killing Companies neurons. are using iPScells cells, may it to possible understand cells be non-diseased with ferentiated into cells. neural comparing thebehavior ofALS By into iPScells.reprogrammed iPScells redif The thenbe can a patient amyotrophic with lateral sclerosis (ALS) be andcan by agiven disease.from cells obtained For skin example, be can thecellsand thencoax thetissues to become that are affected make aresearcher Thus, can iPScells apatientstudying. from are they disease whose patients from cellspluripotent directly tomake researchers for develop of supply possible an it ample cells stem ethical issues also but associated embryonic with anyto become cell in thebody. cells These avoid thenumerous induced pluripotent (iPS) stem cells, which have thepotential called state allows adult cells to converted to be an embryo-like to as “reprogramming.” referred technology Reprogramming cally. recent with to possible advances in a be This appears at discovery histori an earlierpeutic done timethan has been aim isOur to into patients put andthera ofdrug theprocess Petri dish. atdiabetes that (T2D) apatient givealook in a researchers 2 ofchronic models diseases suchnovel as type laboratory FOCUS SCIENTIFIC REPORT : The goal of this laboratory is to ofthis develop goal andvalidateThe laboratory

2 010–2011 mouse ear cells. Panel C: Neurons (arrows) differentiated from mouse iPS mouse cells. from (arrows) C: Panel cells. Neurons ear mouse differentiated cells (arrows) iPS of adult B: Panel cells. from Colonies ear generated mouse A:Panel Adult mice. from obtained cells adult of redifferentiation and reprogramming of example An - - - Recent key in ourlab developments include: reveal secrets. diseases these iswith andiPScells unprecedented, likely will to unlimited work with quantities patients ofcells from derived ofthecell (patient). donor mental history Still, theopportunity environthe how well isand it unknown theiPScells will reflect andenvironmental ofgenetic they amix result factors, from and Alzheimer’sbecause disease.are difficult diseases These in usingested iPScells diseases such complex as T2D to study which involve involving lab is Our asinglemutation. gene inter spinal muscular atrophy (SMA) familial or (FD), dysautonomia Board of Regents IndustrialProgram. Ties Regents of Board Research by thisgrants in is funded the from laboratory Louisiana 4. 3. 2. 1. D. Ph. Gao, Ru Team:Research Ph D. J. Eilertsen, Kenneth Faculty:

thought in theepigenome not to exist andredundancies previouslywith is associated efficiency (PEM), memory epigenetic reprogramming which limits mechanism ofa Identification contributing persistentto andT2D obese including ofpatient types, cellsprogenitor avariety from human to muscleDevelopment ofmethods reprogram colony formation of iPScell inhibitors improves theefficiency deacetylase Demonstration that epigenetic priming using histone to iPScells (see figure) the Gtl2 imprinted andare efficientlyreprogrammed locus express that ears mouse cellsDemonstration from obtained PENNINGTON RESEARCH CENTER BIOMEDICAL - - BASIC RESEARCH 23 - - ) - gene gene Sfrp5 - Mest 010–2011 2

hydrolase family a/b in growth in and develop is strongly is associated with ), an imprinted gene known imprinted an ), in adipose tissue after adipose in tissue feed in the adipose tissue of adult of adult the adipose in tissue Mest Mest Mest belongs to the the to belongs SCIENTIFIC REPORT SCIENTIFIC Mest ). Studies are under way to determine the mechanisms the mechanisms determine to under are way Studies ). Bmp3 Schematic represents potential roles for mesoderm-specific transcript protein protein transcript mesoderm-specific for roles potential represents Schematic within resides adipocyte. MEST the in expansion mass fat modulating in (MEST) lipid enhance may it where complex (ER)/Golgi reticulum endoplasmic the storage the accelerating by synthesis of lipid nascent droplets and mature (NLDs) an as function MEST of catalytic putative the Alternatively, (MLBs). bodies lipid epoxide hydrolase may lead conversion to of epoxyoctadecanoic and epoxye Fatty derivatives. diol respective their to EETs) and (EpOMEs acids icosatrienoic acid epoxide derivatives have been shown effect to cellular signaling, possibly (PPARs). receptors proliferator-activated peroxisome with interaction through Mesoderm ( specific transcript the largest showed epigenetic be by mechanisms, regulated to gene in expressionvariation (~80-fold) among adipose tissue depots the mice. in fat mass expansion under conditions of positive energy balance. energy expansion of positive balance. fat mass under conditions either that identifyOur to goal is the epigenetic mechanism(s) directly or indirectly Mest regulate the catalytic determine and to mice function of MEST and how dietary fed expansion excess mice fat mass in it regulates fat. developed recently a mouse these in aid studies, have we To model can be selectively that used to knock out the in adipose tissue. gain weight with associations Other significant show genes that co-regulated are mice in with a high-fating mice diet. signaling These genes the Wnt include antagonist secreted frizzled-related 5 ( sequence protein ment, and we have shown that Mest that shown have and we ment, and the osteogenic antagonist bone morphogenetic 3 protein ( of these genes regulation and the coordinated in involved expansion role their demonstrate an adipose in in to tissue obesogenic These biological studies environment. unravel will mechanisms associated with variations of adiposity, and identify therapeutic as can targets be that evaluated for the pathways obesity. of treatment laboratory supported Na is from the in this grants by Research of proteins and is expressed only from the paternal allele. Many expressed and is Many of proteins only from allele. the paternal establishedstudies a role for have of Health. tional Institutes Faculty: Robert A. Koza, Ph.D. Research Team: Justin Manuel - - - - The laboratory goal identify of this to is epigenetic :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS determinants of obesitydeterminants metabolic and related disorders. velopment can “permanently” an alter individual’s susceptibility obesityto very metabolic life, and related disorders little later in knownis about cause these the biological that mechanisms contributions epigenetic identify potential these To changes. characterized inbred an mouse have strain we that obesity, to obesity in variations large rise to when gives among individuals Global analyses conditions. environmental controlled in reared and with low of gene expression of mice the adipose in tissue after gain being weight high fed energy-rich, an high-fat diet genesassociated with for of the identification several allowed These include mice. obesity in individual among variations in involved genes and genes, developmental genes, imprinted angiogenesis, vascularization, and cytoskeletal signaling, Wnt organization. Because individual within mice our inbred mouse population are essentially genetically identical, hypothesize we of must underlie the regulation epigenetic mechanisms that with the devel associated some of the genes or gene pathways opment of obesity model. this in oping the disease and the nutritional and physical environment. environment. physical and nutritional the and disease the oping status nutritional during de that shown studiesAlthough have tions betweentions devel genetic predisposition to individual’s an Obesity a complex is metabolic syndrome caused interac by Robert A. Koza, Ph.D. Assistant Professor Epigenetics and Obesity and Epigenetics Laboratory 24

BASIC RESEARCH Laboratory Interactions Gene-Nutrient Associate Professor Associate Ph.D. Mynatt, Randall chondrial accumulationchondrial Additionally, oflipotoxic metabolites. may metabolism themito ariseabnormalities in from fuel that data suggest These oflipotoxicand excretion metabolites. cellular export the in increases were insulin on sensitivity tine carni of supplemental Concomitantfeeding. thebenefits with insulinof impaired diabetes, andhigh-fat aging, genetic action: models mouse three in insulin improved sensitivity mentation investigationsOur supple carnitine have that found dietary capacity. improve mitochondrial and signaling insulin in increase an wouldto lead metabolites lipotoxic in reduction this that and export mitochondrial increased and oxidation increased muscle skeletal via within lipid metabolites would reduce carnitine that posits pothesis acids the from mitochondria.oxidized fatty partially possibly and acetyl-CoA L-carnitineof is to transport the mitochondria Another function for β-oxidation. important L-carnitinerequire of across theinner membrane transport for ofL-carnitine. major functions are at two least acids Fatty sized sources. endogenously There or obtaineddietary from L-carnitine is aconditionally essential nutrient that is synthe tion. which are potent inhibitors ofglucose utiliza and acetyl-CoA, (LC-CoA)the cellular acyl-CoAs concentrations oflong-chain improve would by reducing glucose disposal supplementation speculatedthat has andit carnitine been drial membranes, across mitochon moieties ofacyl in theshuttling role a critical L-carnitine diabetes. of plays therapy adjunctive for approach attractive lation in skeletal muscleavery andliver represents such as L-carnitine,supplements, that ameliorate lipid accumu mulation oflipids in skeletal muscle andliver. use ofdietary The accu andtheectopic associated obesity with which is strongly is insulin resistance, prediabetes of hallmark the disease, and 2diabetes is (T2D) aprogressive It isthat well type established stand the basis of obesity and type 2diabetes. andtype thebasis ofobesity stand cellularstudies, physiology, to under studies andnutrition techniquescombining in mice, engineering genetic clinical FOCUS SCIENTIFIC REPORT : This laboratory utilizes an integrative approach approach integrative an utilizes laboratory This Instructor Jingying Zhang, Ph.D.

2 010–2011 The carnitine hy carnitine The ------Shawna Wicks, Ph.D.; Bolormaa Vandanmagsar, Ph.D Bolormaa Ph.D.; Wicks, Shawna B.S.; Tamra Ph.D.; Haynie, Mendoza, Kimberly B.S.; Ding, Dieyun M.NS.; Burmudez, Estrellita B.S.; Steven Bond, Team:Research Zhang,Ph.D. Ph.D., Jingying Mynatt, Randall Faculty: fat diet. However, diet. fat the increasea moderate inmass fat mice when ahigh- were fed (CRAT) to in muscle led ofCRAT in mice. reduction The activity acetyltransferase carnitine of manipulation is the carnitine tal acids tothe of supplemen benefit overall fatty efflux chondrial ofthecontribution ofmito theextent Key to understanding T2D. with associated metabolism in reversing theabnormalitiesoffuel and utilization glucose insulin-stimulated improving at effective is carnitine that theinitialprovide dietary “proof ofconcept” results These efflux. tolerance acylcarnitine andincreased improved with glucose in concert perturbations these reversed supplementation whereascarnitine oral metabolism, drial fuel were mitochon associated rats aberrant with severely obese levels in insulin Low carnitine and resistance. dysfunction drial causative as tomitochon is insufficiency suspected carnitine can AssociationInstitutes the Diabetes National and Health. of Research by grants this in the isfrom Ameri supported laboratory CRAT homeostasis. in mitochondrialenergy as akey enzyme of therole data support These muscle glucose for homeostasis. ofCRAT role adirect andsuggesting in to obesity secondary indicatingof diet, that insulin resistance mice in these is not glucose values andwere responsive less to insulin irrespective PENNINGTON RESEARCH CENTER BIOMEDICAL Crat knockout mice had higher knockout blood - - - - - BASIC RESEARCH 25 - and LIPC,

010–2011 CETP, 2

max training response alleles. alleles. response training max : We examined: We

2 max training responses across nine predic nine across responses training max

2 SCIENTIFIC REPORT SCIENTIFIC max-adjusted VO

2 gene to be associated with maximum weight loss after loss be gene with maximum associated to weight FTO gene loci contribute significantly to plasma HDL-cholesterol HDL-cholesterol to plasma significantly loci contribute gene tor SNP score categories in HERITAGE whites. A predictor categories score tor SNP constructed was in score HERITAGE using 21 coded was SNPs. SNP based Each on the number of high VO Age-, sex-, and baseline VO The number of subjects within each SNP score category is indicated inside each histogram histogram each inside indicated is category score SNP each within subjects of number The There threefold a is bar. range between those who carry the lowest number of favorable number. highest the carry who those and alleles consists of over 8,500 of over consists extracted DNA samples. diluted and Genetics studies on obesity and metabolic comorbidities using genome-wide exome GWAS, state-of-the-art (e.g., techniques sequencing, deep be will resequencing) performed on PCLS subjects. Swedish Obese Subjects Study (SOS) identified or genes from candidate SNPs of associations the and HDL-cholesterol weight with in changes GWAS through after bariatric surgery found two in patients. SOS SNPs We in the the in SNPs found that also We banding surgery. LPL were genes these however, obese morbidly individuals; in levels surgery-induced with changes. associated not the to contribute to Genomics LaboratoryThe Human continues and participates Study projects specific in QuebecGWAS Family consortia. and MAGIC INSHAPE, pursued the GIANT, by the laboratory published44 peer-reviewed and 2011, In 2010 papersoriginal and 7 book chapters. laboratory supported NIH from is the in this grants by Research Heartand American the Association. Faculty: Claude Bouchard, Rankinen, Ph.D., Tuomo Ph.D. Research Team: Mark Sarzynski, Ph.D., M.S., Jessica Watkins, B.S., Kathryn B.S., Cooper, Diana Holmes, M.S. ------: The The HERITAGE Family Study began Study Family The HERITAGE Tuomo Rankinen, Ph.D. Rankinen, Tuomo Associate Professor : We examined the associations of examined of the associations : We transport, hypoxia, and mitochondrial metabo mitochondrial and transport, hypoxia, + /K This laboratory investigates the genetic and mo laboratoryThis investigates + :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS lecular basis of the response to a physically active a physically lifestyle, of the response to lecular basis emphasizing cardiorespiratory endurance, cardiovascular and typedisease, factors, risk 2 diabetes the genetic as well as obesityof its and comorbidities. background molecular and The Pennington Center Longitudinal Center Study (PCLS) Pennington The lism are associatedlism with symptom-limited exercise test duration seven in SNPs found that we adults. in years Conversely, 20 over modify not did genes candidate fitness and/or blood pressure betweenthe associations baseline fitness or of BMIand risk CARDIA in years 20 hypertension participants. over PCLS cohort subjects of all been consists screened who have at data The central 1994. Biomedical since clinic the Pennington subjects partici 14,000 base includes approximately who have studies. clinical in pated The Genomics Human Laboratory has established a DNA bank for the PCLS cohort, currently which HERITAGE Family Study: Family HERITAGE funded In been and has National the by continuously 1992 in The Genomics Human Laboratory cohorts on several relies to pursue its goalthe of defining andgenetic of molecular basis complex traits. results from Recent are four of these resources described here. stitutes of Health (NIH). Onefind of the most interesting recent (NIH). stitutes of Health single nucleotide polymorphisms (SNPs) from candidate genes genes from candidate (SNPs) polymorphisms nucleotide single well years,as 20 cardiorespiratorywith in changes over fitness of gene-by-fitness the associations as and gene-by-BMIinterac CARDIA Fitness Study Fitness CARDIA ings of HERITAGE relates to the genomic predictors to relates of the ability of HERITAGE ings cardiorespiratory increase to regular to response fitnessin A genome-wide performedexercise. study association (GWAS) used HERITAGE in on the sample descent of European of families a panel genomic and revealed 320,000 markers of more than for the genetic component of these accounted markers 21 that of the ability maximaloxygen increase response to in to uptake regular exercise. This illustrated is in the figure. the CARDIA in years 20 hypertension with incident tions over skeletal to genes related at alleles found that We Study. Fitness Na muscle Claude Bouchard, Ph.D. Barton, John W. Sr. Professor, Chair in Genetics and Nutrition Human GenomicsHuman Laboratory 26

BASIC RESEARCH Laboratory Immunobiology Associate Professor Associate D.V.M., Ph.D. Dixit, Deep Vishwa The two major areas two ofinterest are: ofDr.The laboratory Dixit’s obesity. related to aging andinflammation dysfunction immuneand that andimmune systems contribute metabolic to between theinteractions studies Laboratory Immunobiology The decline of immune function. ofimmunedecline function. thatmechanisms thedisruption ofspecific mayprevent the thehealthy evidence emerging suggests lifeThe span. extend ofnaïve to Tcells and enhance in theelderly theproduction fort ofexcessive thecauses address in thymus lipogenesis in an ef andhis collaboratorsDixit are aggressively research pursuing to lack ofnaïveto Tcells. theprolonged with cope Dr. Therefore, is unable thebody vaccination because failures in theelderly and later infections, risk in increased itself lifeofcancers, with naïve Tcells. ofprotective This manifests process production into tissue, lowand isadipose transformed whichin results very healthy individuals, greater than 75% ofthymus is dysfunctional lab that demonstrates by 45 years in ofage metabolically aging ofseveral Dr. recent other organs. data The from Dixit’s Intriguingly, aging ofthethymus health even precedes in good as welife span by age. all keeping equallystrong organ systems thehealthy andextend aging research is to compress morbidity of goal An important persons. immune surveillance in elderly clinical condition that contributesan intractable to reduced mental and, phenomenon for and biology to puzzling date, to naïve produce Tcells remains age advancing with afunda Senescence Immune 2. 1. in aging andobesity. FOCUS

and dementia. and chronic diseases like diabetes, cardiovascular disease, cancer, aging-associated and obesity- leads that inflammation to Inflammation: driven metabolically origin of the identify To elderly. the in immunity to enhanceprotective approaches thymus from andto ofnaïve developdecline Tcell production Immune senescence: To discover mechanisms that the cause SCIENTIFIC REPORT : This laboratory focuses on immune cell dysfunction immune on cell focuses dysfunction This laboratory : The diminished ability ofthethymus diminished: The ability

2 Instructor Youm, Ph.D. Yun-Hee 010–2011 - - tional Institutestional the Coypu Health and of Foundation. Research by grants this in the isfrom Na supported laboratory diseases. againstof drugs obesity-related related lab’s to theDixit may discovery leadto thedevelopment Research, Medicine, NatureMedicine, Immunology, Cell Research, journals including in severalmentaries high-impact findings these addition, were highlightededitorial in com journal, in aleading biomedical published recently were findings These animals. experimental in diabetes 2 type prevent and can insulin resistance NLRP3 inflammasome obesity. Dr. research team the Dixit’s has shown that blocking in inflammation of regulator and sensor molecular important to discover thefirst among thatNLRP3 the inflammasome is an is ofinflammationThis mediators. laboratory the production immune bythat recognized specific regulate“sensors” ers” area is to discover “danger theendogenous signals” “induc or theoverall Therefore, cancers. ofDr. goal research in this Dixit’s severalger disease, diseases like dementia, and diabetes, heart andaging.This chronic inflammation known trig to obesity is inelevated are cytokines tissue levels pro-inflammatory and of Inflammation prevent obesity-induced damage to pancreas and protect against diabetes. panels).left-hand Right-hand panels show that blocking the NLRP3 inflammasome can insulin undergo inflammation-induced fibrosis blue(sky color as indicator of fibrosis, produce that islets pancreatic obesity, advanced in that showing pancreas of Histology M.S. McDaniels, Sarah B.S.; Chavis, Diane B.S; Albarado, Diana B.S.; Ravussin, Anthony Ph.D.; Grant, Ryan Team:Research D.V.M., Youm, Dixit, Yun-Hee Ph.D.; Ph.D. Deep Vishwa Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL which suggested that which suggested additional research in humans : It has been established that thecirculating established : Ithas been Nature Medicine and Circulation Circulation Nature Nature - - . In - - BASIC RESEARCH 27 - Stud b) 010–2011 2

Investigation of the mechanism of the mechanism Investigation SCIENTIFIC REPORT SCIENTIFIC The beneficial properties harnessedbe may Ad36 of : Although Ad36 is associated with an improved lipid lipid improved with an associated is Ad36 : Although To treat diabetes (U.S. patent obtained) patent (U.S. diabetes treat To To determine whether Ad36 contributes to obesity to contributes human in whether determine Ad36 To nonalcoholic fattynonalcoholic pending) (patent disease liver adults and children To reduce accumulation of liver fat in conditions such as as such fat conditions in of liver accumulation reduce To To develop a vaccine to prevent Ad36-induced obesity Ad36-induced prevent to vaccine a develop To

ies with Ad36 are providing a template to determine the role of the role determine of to providing a template are ies with Ad36 obesity microbes human in candidate other and metabolism. Federal laboratory from the funded is in grants this by Research the LLC, Emergency Initiatives, Health Management Vital Agency, of Human and the Nutrition, Advancement for the Mathile Institute Board. Egg American ii. Rationale: Objectives: Ad36 and adiposity and Ad36 Objectives: i. ii. Rationale increased adiposityhumans, in profile and glycemic have can adverse effects.several health - the caus determining Hence, obesity human in role preventive of Ad36 and developing ative are important.measures Objectives: & glycemic Ad36 control new therapies based develop on the action of E4orf1To protein: i. infectionis with Ad36 However, control. glycemic improve to the use developing are impractical we Instead, a treatment. as of its E4orf1 a therapeutic as agent. protein Additional implications: (a) signaling of action modulators new of cell of E4orf1 revealing is with broader the obesity in interest field. and diabetes ( Faculty: Ph.D.; Dhurandhar, Vijay Hegde,Nikhil Ph.D. V. Research Team: Olga Dubuisson, Ph.D.; Rashmi MD, Krishnapuram, Ph.D.; Grant Williams; Paige M. Kennedy - - -

- - - : : In addition to increasing increasing : In addition to Vijay Hegde, Ph.D. Instructor : We showed that Ad36 causes causes Ad36 that showed : We The laboratory goal understand of this to is the :

OCUS PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON F metabolic pathways altered in obesity in altered metabolic certainpathways by infections. Extensive studies as models, conducted well as animal using the E4orf1 that pro indicate and tissues, cells or human animal E4orf1 protein identified of Ad36 as the candidate mentally infected nondiabetic as well as diabetic animals and infectedmentally and diabetic as animals nondiabetic well as who livers. are their in Humans accumulation reduces lipid significantly betterglycemic infected have naturally with Ad36 control compared uninfected to individuals. necessary is adiposity increase to of Ad36 tein and sufficient and disposal. glucose improve to Ad36 improves glycemic control glycemic improves Ad36 glycerides. In humans, natural Ad36 infection with associated Ad36 is natural In humans, glycerides. hypolipidemia. obesity relative and in experi robustly control glycemic improves Ad36 adiposity, Ad36 increases adiposityAd36 The primary of the Infection interest Obesity and Laboratory is effective obesity or treat to discover prevent and to strategies understanding of complete more A comorbidities. its related the varied etiological factors obesity the human to contributing specific lead to and treatments epidemicbetter may manage important regard, if of a subset of this ment In this disease. obesity caused certain is by infections, the strategies required for its con differ cause-specificmay and treatment prevention approaches. conventional available currently from the siderably have obesity-promoting pathogens past two the In decades, 10 been described, our reports including of the first virus, human The a description is of past following typeadenovirus (Ad36). 36 objectives current as well direc as and research discoveries, tions. obesity experimentally in rats, and infected mice, chickens, and reduces serum cholesterol primates nonhuman and tri Nikhil V. Dhurandhar, Ph.D.Nikhil V. Associate Professor Infection Obesity and Laboratory 28

BASIC RESEARCH Inflammation andNeurodegeneration Laboratory Associate Professor Associate Annadora Bruce-Keller, Ph.D. tween IDRP faculty and clinical departments at Health theLSU andclinical departments faculty IDRP tween have clinical collaborationthese studies be extensive fostered this information on from patient gained based registry. Indeed, novel interventions dementia for have recently developed been to designed test annual Several research screenings. projects of>1,600registry than individuals, more with 97% for returning now has alongitudinal IDRP The andnutrition. as metabolism such risk factors and dementia oflifestyle research theroles on aging brain to cutting-edge spearhead in 2008 was established search andPrevention (IDRP) at Pennington which Biomedical, clinical theInstitute arena.with Re Dementia Shefor is working Dr. has recently moved this Bruce-Keller research into the (red).plaques to amyloid proximity NOX-positive in close (black) neurons AD. shows in figure The dysfunction and injury brain mediating in inflammation, and oxidativestress both produces which NAPDH called oxidase (NOX),delineating ofan enzyme therole PROJAD patients. 4(“Aβ on is andNOXfocused in MCI andAD”) cells, in cultured in transgenic ADthology mice, andin human of this themechanisms grant ofamyloid are pa to understand investigatorswith ofKentucky. at theUniversity objectives The AD researchfunding at Pennington in collaboration Biomedical theNationalA large grant Institutes from ofHealth (NIH) is syndrome, diabetes, and diet-induced obesity. disease (AD) andneuroAIDS, as well ofmetabolic as in models ation andcognitive impairment in diseases such as Alzheimer’s how inflammationto use to initiates understand neurodegener tools are all transgenic animal These putand inducible models. in thedesign ofnovel andimplementation cell type-specific in theanalysiscells, ofanimal as behavior welland as expertise niques to manipulate andmeasure signaling redox in cultured Inthatimpairment. time, numerous shehas tech developed and cognitive injury,brain neurodegeneration, in inflammation Dr. has of over in thestudy 20 Bruce-Keller years ofexperience and how such inflammation disrupts brain function. brain disrupts inflammation such how and inflammationdysfunction caused by diseaseis metabolic and FOCUS SCIENTIFIC REPORT : This laboratory seeks to understand how brain to understand seeks This laboratory

2 010–2011

- - - - - Research by the from funds NIH. this in is supported laboratory (NIH). Health review currently at patients, under HIV theNational Institutes of in ofinsulin therole frailty on resistance in age-related project Sciences Center. This thedevelopment includes ofaresearch Pepping Jennifer M.S.; Knight, G. Alecia M.S.; Gupta, Sunita Team:Research Annadora Bruce-Keller, Ph.D. Faculty: that NOX may be important in mediating neuronal and cognitive dysfunction in AD. in dysfunction cognitive and neuronal mediating in important be may NOX that (red) and plaques to amyloid proximity close in are (black) neurons NOX-positive to NOX identify and senile plaques (dense amyloid deposits). Images suggest that processed then brain, AD human from derived tissues formalin-fixed from prepared NAPDH oxidase (NOX) in Alzheimer’s disease (AD). Histological sections were PENNINGTON RESEARCH CENTER BIOMEDICAL BASIC RESEARCH 29 ------L. extracts to sp.

010–2011 improve insulin improve L. increases 2 we have identi

A. dracunculus Artemisia L. action regulates insulin at in vivo, and vivo, in genus representing both closely and both representing genus . dracunculus L. (Russian tarragon) for itsability tarragon) en L. to (Russian SCIENTIFIC REPORT SCIENTIFIC which extracts which by vivo in of Artemisia sp. Faculty: Cefalu, M.D.;William Zhong T. M.D. Wang, Research Team: Ph.D.; Xian Zhang, B.S.; Yu, April Ph.D.; Stull, Yongmei Diana Obanda, Ph.D ment to improve insulin resistance in human subjects. human in resistance dem We insulin improve to ment Our lab, as part of Pennington Biomedical’s National Institutes National part as Biomedical’s Our lab, of Pennington on and for Botanicals Research (NIH)-funded of Health Center of botanical active also is the investigation Metabolic in Syndrome, aspects and other resistance supplements on insulin of metabolic extract an syndrome. funding cycle, evaluated In the initial we of dracunculus Artemisia action. botanical identified insulin This originally was hance from a screening of extracts activity for hypoglycemic the most as mice in supple nutritional a of development the for candidate promising a well-characterizedonstrated that extract of receptor regulates signaling the at insulin cellular 5011) PMI (called level and increases sensitivity insulin fiedand modulated proteins novel several intracellular pathways the extract.by the demonstratedthat our studieshave Specifically, A which by mechanism the cellular level may bethe level may secondary cellular modulating to negative regula PTPases, i.e., and reducing lipid signaling, receptor tors of insulin provided In addition, target we in evidence tissues. intermediates dietarythat supplementation with A. dracunculus whole-body action early-phase insulin in studies. human expanded have two in First, areas. major basedOur investigations botanical screening efforts of our center’s to identifyon the success selected other including are botanical we leads, promising other members of the Artemisia diverse their how unclear it remains species related distantly since biochemical and taxonomical characteristics Second, related. are with of state-of-the-art use metabolomicand proteomic profiling provide in-depth to expanded have investigations we techniques, of action mechanisms of the cellular analysis and comprehensive operative and enhance modify lipid cellular metabolism whilesimultaneously modulating negative regulators receptor of signaling, insulin i.e., complementary as PTPases, liver and muscle skeletal in compo insulin these which botanicals enhance by nents of the mechanism sensitivity metabolic the progression to and attenuate syndrome. laboratory supported NIH is from the in and this grants by Research Foundation. Coypu the sensitivity. Thus, the primarysensitivity. objective projects of our current to is the combined effectsevaluate of selected - - - - -

. Caloric restriction in vivo in Zhong Wang, M.D. Wang, Zhong Instructor and has identified a specifica identified has and vivo in

The primary of our laboratory study mission to is the :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS chemicals that may prove valuable in addressing the underlying addressing in valuable prove may that chemicals metabolic obesity, to contributing parameters pathophysiologic syndrome, and type 2 diabetes. cellular mechanismsoperativecellular in insulin-sensitive that tissues on a whole- resistance of insulin the development to contribute phyto evaluate In addition, to body our goal is humans. in level One most for of the patients with goals type desirable treatment 2 diabetes increase sensitivity to is insulin dietary beenOur has lab include evaluating that interventions the evaluating addition in to minerals, of trace the intake altering effectspecific on phytochemicals. withcollectivedate Our to work the trace has mineral demonstrated chromium (Cr) that mineral this action enhance insulin may and lipids myocellular observations that combined the Thus, ing strategies to improve insulin resistance by pharmacologic or by resistance insulin improve ing strategies to supplementation representsnutritional a very attractive approach of type treatment the to 2 diabetes. supplementation. Cr to phenotype responsive is that humans in subject demonstratedthat phenotype,have we Cr not Specifically, provided evidence have We response. status, clinical determines receptor signaling in enhance insulin skeletal Cr may that muscle effectaction Cr’s and that theat whole-body insulin on is level enhancedrelated sensitivity to insulin in muscle, and not secondary production. hepatic glucose enhanced to In pursuit of mechanisms intracel reducesCr effect, demonstratedforthat this have we Cr and that and liver muscle skeletal human in content lipid lular modulates negative regulators receptor of signaling insulin (protein preclinical in muscle skeletal in tyrosine PTPases) phosphatases, i.e., studies. provide a strong Cr PTPases for by explor modulated rationale are and exercise greatly improve insulin resistance, but it is difficult butit is resistance, insulin improve greatly and exercise design lifestyle long-term these Therefore, changes. maintain to actions linking on Cr’s metabolism lipid mechanisms ing the cellular beneficial effect overall molecules Cr’s and signaling to oncarbo current proposed. as metabolism humans in Thus, the lab’s hydrate Cr modulates preciselyby which to definefocusthe is mechanism the as muscle in signaling receptor metabolismlipid and insulin of actionprecise mechanism for Cr. William T. Cefalu, M.D. William T. Douglas L. Sr. Manship Professorship in DiabetesProfessorship John S. McIlhenny Botanical Research Laboratory Botanical Research McIlhenny S. John 30

BASIC RESEARCH Leptin Signaling intheBrain Laboratory Assistant Professor Assistant Ph.D. Heike Münzberg-Grüning, neurons regulate energy expenditure and feeding behavior. and feeding neurons regulate expenditure energy in galanin deletion LepRb neurons to demonstratethat Gal-LepRb hypothalamus andthebrainstem. We mice generated genetic with andlateral 2). neurons are in found thedorsomedial Gal-LepRb galanin oftheneuropeptide (Gal) (Figure by their co-expression neurons another ofLepRb population characterized We further weight regulation. body sociated as and action leptin thermoregulatory that mediate mechanisms We theneuronalandinvolved circuits are currently exploring food intake. ficientweight affecting without body to reduce hypothalamus inis thedorsomedial suf thatfound leptin action features thatexpenditure, we promote should weight Indeed, loss. tissue adipose Brown is to able generate heat andincrease energy tissue (Figure 1). adipose brown the via action leptin regulatory thermo that hypothalamus mediates dorsomedial the in neurons We recently discovered asubpopulation ofLepRb-expressing homeostasis. tion ofenergy populations to contribution LepRb regula ofthese an important large neurons, populations thus of suggesting LepRb-expressing andcontain expenditure to and energy regulate circuits feeding known lateral are hypothalamus and dorsomedial the example, For and their relative contribution to overallis unknown. leptin action neurons have studied, not been ofLepRb-expressing majority The in role overallleptinspecific action. their dissect and populations neuronal LepRb-expressing distinct of thefunction to explore is it important propriate leptin actions, to interventions inapIn order pharmacological these enable for offalling leptin levels. because sensation hunger increased obtained dieting from evokes energy-saving mechanisms and are insensitive to leptin therapy. Conversely, weight loss body However, have patients obese already most high leptin levels and receptor (LepRb). in humansnull leptin its for or rodents or obesity gia andmorbid regulator balance, ofenergy by as severe demonstrated hyperpha tissue, white adipose from produced isLeptin, akey ahormone energy expenditure, and body weight. and body expenditure, energy intake, theregulation on offood neurons andtheir impact target FOCUS SCIENTIFIC REPORT : The goal of this laboratory is to ofthis investigate goal The laboratory novel leptin

2 010–2011 ------Supervised UndergraduateSupervised Research [SURE]). Experience ProgramExperimental to Stimulate Competitive Research [EPSCoR], Science (Louisiana Louisiana FoundationReagents and of Board Research Center,the Nutrition Obesity and as wellas the National Health, the Center Research Biomedical of Excellence (COBRE), and Research by Institutes the National this in is supported laboratory of populations. LepRb specific of these tracingneuron-specific the neuron to identify connecting circuits neurons, and(c) virus-driven mechanisms ofLepRb-expressing regulatory to models identify mouse subpopulations in reporter LepRb of specific ior,( b) visualization behav and feeding energy homeostasis in subpopulations LepRb-expressing contribution ofthese to determine the LepRb deletion) neuronal inhibition, stimulation, neuronal neurons (e.g.,LepRb of perturbation cific clude: (a) neuron-spe in These neurons. LepRb-expressing of subsets the specific investigate to tests tools andbehavioral genetics molecular We use several circuits).reward behavior (e.g., food mogenesis) or feeding tissue ther adipose (e.g.,penditure brown ex energy regulate neurons Gal-LepRb which by mechanisms the exploring We arecurrently Sarah Gettys Sarah Tu-Anh Bui; Kelly Nguyen; B.S.; Laque, Amanda Team:Research Ph.D.Heike Münzberg-Grüning, Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL - - - - - regulate brown adipose tissue thermogenesis. that neurons of (red tissue stain) indicator adipose an as brown the tracer viral from atranssynaptic, with labeled are that hypothalamus (green stain) dorsomedial the in Figure 1: DMH. rons). fornix, fx= vDMH=ventral DMH, dDMH=dorsal neuropeptide galanin (green stain) (Gal-LepRb neu the of co-expression by their (DMH) characterized are hypothalamus (PFA) area perifornical dorsomedial and pSTAT3, leptin-induced as ized stain) red the in nuclear Figure 2: A subpopulation of LepRb neurons (visual- We identified a subset of LepRb neurons neurons of LepRb a subset We identified - BASIC RESEARCH 31 - - 010–2011 2

experiments, high SCIENTIFIC REPORT SCIENTIFIC can Diabetes Association and the National Institutues of Health. and National Diabetesthe Institutues Association can in dorsalin neurons. In in root ganglion vitro glucose-exposed transfected cells Schwann cultured human stress, si-RNA com reduced oxidative displayed with NHE-1 non-transfectedpared with si-RNA-transfected non-silencing or figure). (see cells Laboratory of Diabetes Complications The Mechanisms also is the roles of peroxynitrite evaluating in and protein involved stress, reticulum diabetic endoplasmic in as well as nitration, neuropathy and other diabetic complications. laboratory supported Ameri is from the in this grants by Research Representative Western blot analyses of NHE-1 (A), 4-hydroxynonenal aducts (C) and and 4-hydroxynonenal aducts (C) (A), NHE-1 of analyses blot Western Representative siRNA-transfected non-sicencing and siRNA-transfected, NHE-1 and (E), proteins nitrated human Schwann cultured cells mM or in mM 1 - non-transfected 5.5 30 glucose. cells transfected culturedcultured cells 2 - NHE-1 glucose; mM 3 - non- glucose; in in 5.5 5.5mM transfected cultured cultured cells cells in in mM mM 4 - NHE-1-transfected 30 30 glucose; transfected 5 - cells glucose; with non-silencing cultured siRNA mM Mean glucose. in 5.5 ± vs. vs. non-transfected cultured cells **p<0.01 mM ##p<0.01 glucose; n = 3. inSEM, 5.5 non-transfected cultured cells in mM 30 glucose. Faculty: Ph.D. G. Obrosova, Irina Research Team: Sergey Lupachyk, Ph.D.; Ph.D.; Pierre Watcho, Hanna Shevalye, M.Sc.; Roman Stavniichuk, M.Sc.; B.Sc. Oleksandr Obrosov, ------The laboratory goal understand of this to is the patho -exchanger-1 (NHE-1), which plays a key role in control role control in a key plays which (NHE-1), -exchanger-1 : + /H

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS tions of diabetes, especially of diabetes, neuropathy. tions genesis of and identify of and genesis new therapeutic targets for the complica In another project,In another and activation overexpression found that we of Na creased sorbitol pathway activitycreased sorbitol pathway the aldose by inhibited and is reductaseincreased foundthat also We fidarestat. inhibitor phosphorylation clearly MAPK, is ERK, of p38 SAPK/JNK and inhibition nerve 12/15-LO manifest ciatic in of diabetic mice. gene deficiencycounteracted diabetes- with CDC or 12/15-LO and ERK, MAPK phosphoryla but SAPK/JNK, not p38 associated were obtainedglucose-exposed high in findings tion. Similar suggests which rel cells, clinical Schwann cultured human of our work.evance We previously demonstrated previously important an We role for the 12/15-li poxygenase (12/15-LO) in nerve conduction deficit, small nervesmall in deficit, conduction poxygenase (12/15-LO) sensorynerve fiber dysfunction, large of atrophy and axonal Our fibers(DPN). diabeticin peripheral myelinated neuropathy suggest mice new studies streptozotocin-diabetic in C57B16/J results from DPN in in pathway activationthta the 12/15-LO of of intracellular pH, contribute to DPN in both in type DPN to pH, contribute type 1 and of intracellular 2 diabetes. Our experiments performed were streptozotocin- in in mice, diabetic rats, rats, ZDF and leptin-deficient(ob/ob) cariporide,as used we the specificwhich of NHE-1 inhibitor leads mechanism this found that We mice. NHE-1+/- in as well dysfunction, small neurovascular nerveto slowing, conduction We and diabetic pain. neuropathic sensory fiber neuropathy, important an plays of the NHE-1 upregulation found that also role the intraepidermal in nerve sen small of fibermarker a loss, sory with inhibition nerve fiber degeneration,NHE-1 andthat cariporide least at partially restored variable this caused (i.e., sensorysmall Our nervefuther studies fiberregeneration). through DPN to contributes upregulation NHE-1 that revealed end-productadvanced glycation and oxida formation (AGE) tive-nitrosative stress. Cariporide treatment reduced concentra stress.tive-nitrosative Cariporide treatment of methylglyoxal-derivedtions 4-hydroxynonenal AGE, adducts, and nitrotyrosine as nerve well as sciatic in cord, and spinal 4-hydroxynonenal adduct immunoreactivities nitrotyrosine and Irina G. Obrosova, Ph.D. Professor Mechanisms Complications Diabetes of 32

BASIC RESEARCH Neurobiology andNutritionLaboratory George H. Bray Professor Ph.D. Berthoud, Hans-Rudolf hypothalamus and their projections tohypothalamus theventral tegmental and their projections intake.of food Specifically, in neuronsthe laterallocated orexin controls overriding ofmetabolic in thereward-driven tant role balanceregulation and energy may ofappetite play an impor hypothalamic to involved neuronsbe known peptidergic in the ofthis nucleus to parts that from strated anatomical projections behavior, in reward-driven role crucial its for we have demon nucleus accumbens andmice, in rats area abrain recognized Using manipulation chemical of potential addiction. ofthe food interested in pathways theneural andbehavioral manifestation reward behavior, intake, food increased andobesity. We are (4) combinations mechanisms ofthese alterations cause in food state, or obese of the (3) foods, effects energy-dense secondary (2) rewardbrain system, to palatable, repeated exposure high- that (1) hypotheses competing the in differences predisposing We environment are testing the and lifestyle. themodern with availability,food palatability, and associated energy density control by increasedoverpowering systems ofhomeostatic in at ofthebrain therole we are looking project, In one with the increasingly obesogenicenvironment. maininterface the representing brain, emotional and warding, thecognitive, with interact hypothalamic circuits re regulatory signals interestedWe in how metabolic andthe are particularly syndrome. 2diabetes, andthemetabolic ment ofobesity, type balance,tion ofenergy andtheir involvement in thedevelop controlmechanisms ofappetite, regula ofnutrient detection, FOCUS SCIENTIFIC REPORT Our laboratory has interest ageneral in theneural laboratory : Our

2 Instructor Ph.D. Zheng, Huiyuan 010–2011 - - - - - pharmaceutical industry. InstitutesNational by Healthprivate and of the from funding Research by grants this in the isfrom supported laboratory drugs. available currently than effective that andbehavioral approaches are pharmacological moreless” leadto should “knife “how surgeries bariatric work” Knowing changes in thegut. andfunctional we are assessing structural involved choice, intake, in energy and food and meal patterns, vein, andliver in changing functions neural hepaticportal tract, gastrointestinal the in chemosensors and mechano- spinal and vagal of role possible We arethe testing effects. the beneficial about bringing in brain to the input sensory, vagal particularly balanceenergy controls. We are also ofneural, testing therole intake and offood aspects andhedonic regulatory homeostatic variousareas on brain lating involved guthormones in the ofaltered circu is dueto theaction surgery weight loss after Wesurgeries. are testing thehypothesis that thesustained sleeve gastrectomy lar Roux-en-Y andvertical bypass gastric surgeries,in bariatric particu 2diabetes after andtype obesity hormonal and neural mechanisms responsible for reversing thepowerful identifying we haveIn another started project, environmentobesigenic behavior. appetitive on ofthe to andpharmaceuticalioral theimpact strategies lessen to generate newbehav are expected studies ofthese results induced in the nucleusThe accumbens. effects feeding for sary to neces be seem system, rise to dopamine themesolimbic neurons dopamine thatarea, nucleus amidbrain harboring give Townsend, B.S.; Michael Mumphrey, B.S.; Abby Hué Abby Mumphrey, B.S.; Michael Townsend, B.S.; Leigh Robbie B.S.; Patterson, M. Laurel Ph.D.; Mariappan, Nithya Ph.D.; Lenard, Natalie Ph.D.; Shin, C. Andrew Team:Research Ph.D.;Hans-Rudolf Berthoud, Huiyuan Zheng, Ph.D. Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL - - - - - BASIC RESEARCH 33 - - 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Faculty: Ph.D. Morrison, Christopher Research Team: Denise Fernandez, B.S.; Scott Ph.D.; Reed, D.V.M., Henagan, Ph.D. Tara In addition to the regulation of energy intake, a large body large a of energy the regulation intake, In addition to supports of literature intake. a specific of protein regulation High-proteindiets food low-protein diets suppress while intake increase food will and voluntarily animals intake, self-select adequate an between consume they diets multiple ensure to meet to intake protein balance they How of protein. amount that indicate data Our recent need unclear. currently protein is acid leucine actsthe amino food suppress the brain in to intake and it that does so in part influencing by the same populations the effects mediate that of brain neurons other and of leptin leucine that Theseindicate data figure). (see signals nutritional work, our current and in are we of protein, signal be a key may specificallytesting whether representsleucine a physiological and selection. inter intake also are of protein regulator We between and energy ested the relationship intake in protein dietary in and whether alterations or the neural intake protein detect that can be protectmechanisms protein used to against obesity. laboratory supported Nation from is the in this grants by Research Medical Foundation. Pennington of and Health the by al Institutes - - - The goal of this laboratory is to investigate neural neural The laboratory goal investigate of this to is :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS mechanisms that control food intake in response to changes changes response food in to control intake that mechanisms status. nutritional in ute to weight gain and obesity. gain weight to ute ance their need their ance for macro- multiple and micronutrients against this the uncertainties is How of foodand quality. availability Laboratory The Neurosignaling task accomplished? complicated whereby the brain detectsfocuses on the mechanisms changes food regulate to information status nutritional in and uses this and selection.intake circuits One the neuronal of focus is area leptin as hormones such by molecules utilized signaling and and Our insulin. data demonstrate voluntarily lean that animals a following food their reduce and rapidly lose weight intake seem animals lean words, In other period gain. weight of forced adaptive this that Our gain. indicate data weight excess resist to reduction in food impaired is intake lack that leptin in animals signaling, suggesting intact that leptin required signaling is that Considering for effective an gain. weight defense against obesity these with signaling, of brain a loss associated leptin is observations action of leptin the loss contrib suggest that may The regulation of food intake is oneThe of the most regu of food is regulation essential intake latory Free-feeding must bal phenomena biology. in organisms Christopher Morrison, Ph.D. Morrison, Christopher Associate Professor Neurosignaling Laboratory Neurosignaling 34

BASIC RESEARCH andAdipocyteNutrient Signaling Sensing Laboratory Professor Ph.D. W. Gettys, Thomas humans with metabolic syndrome. Our recent studies make recent Our studies a syndrome. humans metabolic with oxidation fat hepaticlipid contentincreased andreduced in ity. We methionine restriction recently showed that dietary enhanceinsulin and sensitiv health, metabolic of biomarkers that responses ofphysiological reduce adiposity,series improve ahighly produces integrated methionine restriction Dietary syndrome. metabolic and acluster called ofcomorbidities development lipid metabolism, ofinsulindisordered resistance, and accumulation tissue ofexcess linked adipose is to strongly ofachronic balance, positive energy is theproduct Obesity MethionineDietary Restriction Enhances Insulin Sensitivity. Current Investigations tissue-specific insulin sensitivity. sensitivity. insulin tissue-specific restriction that improve overall and signals generated by dietary methionine the of translation and detection in and communication networks involved Anatomical illustration of the tissues restriction to enhancement ofinsulinrestriction sensitivity. signalingderlying methionine mechanisms that link dietary FOCUS SCIENTIFIC REPORT : The goal of this laboratory is to ofthis investigate goal The laboratory theun

2 010–2011 - - tutes American Association. HealthDiabetes and of Research by grants this in Insti the from is lab National supported differentetiologies? insulin preexisting resistance reverse of methionine restriction beneficial? equally (c)dietary Will be acids amino essential of other methionineto or restriction specific would restriction methionine of methionine sensed? (b)effects Are thebeneficial are: (a)in thelaboratory in is How andwhere thereduction addressed being significant unanswered most questions The is enhancementresponses aprofound ofinsulin sensitivity. responses to insulin. resultnet specific The of these coordinated tissue- amplify and lipids circulating tissues to reduce ripheral pe in effects direct through insulin enhances and sensitivity balance on through energy centrallyadiposity mediated effects reduces methionine restriction compelling that case dietary Van, B.S.; Yagini Joshi, B.S.; Manda Orgeron; Cory Cortez Cory Orgeron; Manda Yagini B.S.; Joshi, Van, B.S.; Nancy Stone, Ph.D.; Kirsten Ph.D.; Suk Chang, Ji Ph.D.; Plaisance, Eric Team:Research Ph.D. W.Thomas Gettys, Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL - - BASIC RESEARCH 35 - - - - 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Faculty: Ph.D. Barnes, J. Maria Research Team: Stewart, J. Lyndsey B.S. sity of MOR is significantly increased in the arcuate nucleus; and and nucleus; sityincreased thearcuate of MORin significantly is nucleus MOR colocalized the arcuate are within on neurons (2) agouti-related Y, the orexigenic (neuropeptide contain that and anorexicprotein) (pro-opiomelanocortin) peptides that energy homeostasis. a role maintaining in play The possibility contribut population are receptor exists this in changes that development the potentiate receptors opioid mu increased er The resultsof obesity. obtained from these studies can provide a target potential attenuate and to the increased overeating fat preference observed who obese. are humans in laboratory supported Na from the is in this a grant by Research of Diabetes, and Kidney Digestive Diseases. tional Institute Data from our laboratory have demonstrated that within the fromData the within demonstrated our laboratory that have opioid a populationof mu receptors is there nucleus arcuate animals population makes receptor Activation of this (MOR). food their and selectivelyincrease intake dietary their change fat, in independent a diet high original of their preference to dietary Our receptor laboratory preference. this in interested is obese, the den animals make when we population because: (1) and increased fat preference observed the overeating ing to in obese animals. The studies our laboratory in (1) determine: designed are to obese in increase opioid causes mu what receptors to animals; opioid mu which receptorsmake by mechanisms potential (2) wheth (3) and hyperphagic fat preference; increase and animals Panel A: RepresentationPanel A: of the area of the brain (medial that arcuate used was to nucleus) identify the mu opioid receptors. Panels B, and C, High-power D: magnification(40x) of the arcuate nucleus. Panel B shows a neuronimmunostained for Agouti-gene Related Peptide Panel a representative C is of a neuron that positively is immunostained(AgRP). for mu opioid receptors. Panel D shows an overlay of panels B and which C, demonstrates that mu opioid receptors are located on AgRP neurons within the arcuate nucleus. - The laboratory goal identify of this to is and char :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS acterize the changes within the central nervousacterize the central within changes the system that obesity. diet-induced of development the to contribute Feeding and energy behavior homeostasis in organized are nervousa complex, the central fashion in system hierarchical about Information metabolic sensed status is via a (CNS). variety an is The of hormonal hypothalamus and neural signals. of the nucleus important The of these arcuate signals. integrator of particular is Neural the circuits interest. within hypothalamus both orexigenic contain and anorexic nucleus peptides arcuate important an been play to shown have role energy in that homeostasis. Maria Barnes, J. Ph.D. Assistant Professor Nutrition and Neural Signaling Laboratory Signaling Neural and Nutrition 36

BASIC RESEARCH Nutritional Neuroscience andAging Laboratory Professor Ph.D. Ingram, K. Donald of CR, including improved motor and cognitive function. We including improvedof CR, motor are andcognitive function. unhealthy similar diets, to consequences theeffects of high-fat against improves to andprotection MH diets insulin function centrations ofthe sugar (MH). mannoheptulose Addition of containing high con islead compound an avocado extract stepThe in glucose metabolism thefirst ofhexokinase, tors we ourcurrent focus, are examining inhibi As in CR. observed toglucose in order metabolism induce cellular responses have several exploring been compounds designed to inhibit dieting. ofrequiring without CR We would thebenefits provide “CR Inprinciple,these mimetics” bymechanisms CR. activated cellular stimulate pathways signaling can to invoke protective research to evaluate pharmaceuticals andnutraceuticals that ficult to maintain. address thisTo challenge, we have initiated in animals, may dif diets such be stringent observed those (CR)paralleling restriction mightcalorie health provide benefits in humans have conducted Although studies indicated that this improve function. and disease andpathology,age-related enhance responses, stress levels normal below 50% markedly increase lifespan, reduce to 30% reduced calories with nutritious diets models, rodent Invarious aging, on longevity, andfunction. diets of low-calorie we focus, have investigatingeffects been primary thebeneficial playing nutrition our with amajor role. As factors, vironmental en and genetic interacting multiple through isAging regulated brain andbehavioralbrain function. disease andpromote late-life aging andage-related retard velop interventions nutraceutical andpharmacological that FOCUS SCIENTIFIC REPORT : The goal of this laboratory is to ofthis discover goal The laboratory andde

2 010–2011 - - - - - Research Center (NORC). Institutesthe National Health sponsored of Nutrition Obesity and CenterNational Alternative and forComplementary and Medicine, America, Juvenon, Inc., the Association North of Wild Blueberry Research,Foundation forMedical the Association, Alzheimer’s the Research by grants this in the isfrom Glenn supported laboratory brain glucose and metabolism. memory on cial effects acetyl-L-carnitine and alpha-lipoicacid, benefi by its exploring supplementJuvenon, containinginvestigation ofthedietary toIn addition nutritional interventions, up an we are wrapping signaling pathways required to provide long-term protection. thecellular andidentify response ofthehormetic the kinetics Currently, to map weattempting are products. many botanical exercise, of CR and as well as theeffects to explain proposed is andit agrowing research field protection, creases long-term this to that to response term short-term stress used describe in days, we oxidative noted increased “Hormesis” stress. ofanimals theresponses 2 only for diet examined theBB on Paradoxically, we when antioxidant systems. upregulated and radicals of oxygen production differentmodels decreased by to 12 we noted greatly in attenuated pathophysiology weeks, 6 for was diet fed theBB When ofhypertension. models rodent (BB: 2% by blueberries supplementedweight)of adiet with in effects beneficial the weexamining are Medicine, Veterinary Dr. Francis, of Joseph Professor at Associate School theLSU in collaboration with conducted In other nutritional studies which we have shown that CR attenuate can pathogenesis. ofAlzheimer’s including transgenic models models, disease, in ourfindingsMH of severalto other rodentcurrently extending Carrie M. Elks, Ph.D.; Jennifer Dowden, M.S. Dowden, Jennifer Ph.D.; Elks, M. Carrie Team:Research Ph.D. Ingram, K. Donald Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL

is the the is - - BASIC RESEARCH 37 ------010–2011 2 electron spin electronspin

SCIENTIFIC REPORT SCIENTIFIC Faculty: Krisztian Ph.D. Stadler, Research Team: Cleland B.S.; Ellen Howard, Ruggiero Christine Application of a novel immuno-spin trapping approach in vivo in renal tissues. The green green The tissues. renal in vivo in approach trapping immuno-spin novel a of Application kidneys mouse high-fat-diet-fed in oxidation protein and radicals protein shows staining accumulating in the glomeruli after weeks of feeding. 16 The spin trap DMPO adminis is before mice to tered then sacrifice, an anti-DMPO antibodyis applied on to fixedtissues localize protein radicals. The detection The approach with is confocal microscopy. allows us “visualize”to radicals for the first time space vivo in in and time. Electron spin resonance (ESR) spectroscopyElectron been (ESR) has resonance spin gold the standard characterize to these free radical processes, it de as the sensitivity while mary of a reactive species unambiguously, identification the makes approach trapping immuno-spin novel of the targets localization and their possible. the cell within of toxic the accumulation how (a) in interested are we Currently, lipid metabolites interferes with receptor the signaling insulin duringpathway hyperglycemia, hypertension, and heart failure and participates in the development resistance; (b) of insulin overload lipid and stress oxidative mitochondrial renal how and pathology how redox imbalance obesity; in to relate and (c) lipotoxicity (albumin-bound fatty affects acids, ceramides, etc.) bioenergetics as such kidney various in and redox cells balance podocytes cells. tubular or proximal Founda laboratory funded Pennington is in the this by Research tects or the radicals radical adducts their through not directly, fingerprintmarkers. use in theof vivo Through methodologies able are trapping, and spin we (EPR) resonance directlyto and specifically detectincreased free radical produc bodycom In addition, the cells. with tissues, tion in and fluids, confocal and immunological (e.g., techniques of EPR bination a detailed for immunohistochemistry), the search microscopy, and the localizationsources and their of reactive intermediates can The be of uniqueness achieved. targets or proteins) (lipids allows trapping spin vivo in with spectroscopy combined EPR identify to us free radical metabolites and the participating pri a pilot by of Health, National from the Institutes a grant by tion, of Diabetes and and Digestive from a National Institute grant and Obesity Nutrition Kidney Center Diseases-supported Research project, Board of Regents and a Louisiana program Supervised by Award. Experiences (SURE) Research Undergraduate - -

No DMPO HF 16HF week + DMPO 1DMPO g/kg

The laboratory goal understand of this to is the role : LF 16LF week + DMPO 1DMPO g/kg Secondary only Secondary

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS of free radicals—reactive oxygen and nitrogen species—in and symptoms, with diseases an of various the development emphasis on diabetes, obesity, resistance. and insulin Diabetes mellitus is a chronic metabolic a chronic and oxida is condition, Diabetes mellitus The Oxidative Stress and Disease Laboratory and Disease The Stress Oxidative focuses on the exact a rolethe in can free play that radical mechanisms ultimately conditions, above-mentioned the of pathogenesis contributing resistance insulin to or leading damage, tissue to metabolic and imbalance, complications. tive stress oftentive has been of the pathogenesis in implicated radical production Free the disease. been has demonstrated in both type 1 and type the to and possibly 2 diabetes contributes diverse through mechanisms. of complications development the detailed responsible for the patho mechanisms However, of certaingenesis metabolic diabetic complications, imbalance resistance,and are insulin still largely unknown. Krisztian Ph.D. Stadler, Assistant Professor Oxidative Stress and Disease and Laboratory Stress Oxidative 38

BASIC RESEARCH Protein andDevelopmental Deficiency Biology Assistant Professor Assistant Ph.D. Sutton, Gregory malnourished mothers. mothers. malnourished from offspring in T2D for risk the modulating in rhythm cadian cir ofaberrant therole in utero,circadian rhythm andidentify that influence targets molecular the to identify seeks laboratory ing cardiovascular disease later andT2D in life. Work in my utero that resulting have offspring risk develop for increased ofahealthy lifespan, malnutrition with in part is an important in theWesterndisease known state World. Maternal Nutrition Type acknowledged widely ofthemost 2diabetes in (T2D) one protein deficiency utero.in protein deficiency to inexposed fetuses syndrome development ofmetabolic to the contributes rhythm circadian aberrant how understand is to ofmy andthegoal processes, laboratory ous biological Circadian rhythm plays asignificant Circadian rhythm in numer role FOCUS: SCIENTIFIC REPORT

2 010–2011 - - - Center. Research Association,Nutrition NIH/NIDDK Diabetes Obesity and Research by thisgrants in is funded the from laboratory American mothers. of malnourished in theoffspring to prevent observed theT2D we drugs hope standard with Rev-erba targeting By function. genes involved in inflammation, lipogenesis and mitochondrial throughtabolism theregulation ofdownstream ofanumber is linkedfollowing maternal to me malnutrition. Rev-erba likely as themost candidate risk T2D increased for laboratory (Rev-erba), gene ourby identified circadian rhythm has been disease. riskAdditionally, metabolic increased for specific one that inhave offspring profileand an altered circadianrhythm uteroin results thatmalnutrition suggest protein deficiency ofmaternal using model arodent ourlaboratory Data from Armand V. B.S. Armand Albarado, Diana Centanni, B.S.; Team:Research Ph D. Sutton, Gregory Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL - BASIC RESEARCH 39 -

------using 010–2011 2

Panel A: ElectronPanel A: micrograph image of amyloid fibrils. separation CE Panel B: of amyloid A-beta individual fibrils.PanelCapturedC: and fibrils amyloid concentrated dielectrophoresis. gradient by using a vivo mousein Artemisia dracunculus Artemisia SCIENTIFIC REPORT SCIENTIFIC

(C) primary and culture muscle skeletal human These resistance. studiesmodel providing essential are of insulin understand to data of action the mechanism of botanical extracts in improving sensitivity insulin the at molecular level. the from grants supported by is laboratory this in Research Medical Pennington NIH, the the and of Regents, Board LSU Foundation. teomics technologies, we have demonstrated that modulation of modulation demonstrated that teomics technologies, have we transportinflammatory are thecentral and GLUT4 pathways fac enhances PMI-5011 by which mechanism cellular torsthe defining sensitivity are insulin states in of metabolic We dysregulation. spectrometry-based mass utilizing now targeted proteomics and metabolomics in vitro approaches test these pathways to L., termed PMI-5011, improves insulin action. global pro insulin Using improves L., termed PMI-5011, structural These broadly information. are techniques applicable misfolding and ag with protein associated diseases various to gregation. and botanicals for assessment metabolomic and Proteomic syndrome. metabolic parameter pathophysiologic the major is resistance Insulin defines metabolicthat syndromeand typediabetes. 2 Insulin a prediabetic it typically is as resistance condition, presents 5 to of type prior the onset and diagnosis years to and 2 diabetes 10 factor dis a contributing is of cardiovascular the development in eases. A variety modu of botanical postulated are treatments to the exact these which by mechanism action; however, insulin late extracts In collabora unclear. is modify levels insulin and glucose on for Botanicals and Metabolic Research tion with the Center Biomedical, funded In the National Pennington by at Syndrome which by mechanisms evaluating are we (NIH), stitutes of Health a well-characterized extract ethanolic of Faculty: Ph.D. Kheterpal, Indu Research Team: Scherp,Peter Ph.D.; Jennifer De Guzman,Ph.D., Ginger M.S.; Ku, Jacob Myers, M.S. ------, but in vitro (B) Protein aggregation and abnormal aggregation and Protein

(A)

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS: The laboratory goal and of this the development is application of analytical study to techniques and biophysical biology. disease tion kinetics and provide insight into the extenttion kinetics into of secondary and provide insight or these concentration methods do allow not structure; however, structures aggregate of various manipulation the amyloid within mixture. and applying developing now are capillary Therefore, we electrophoresis and microfluidics-based (CE) separation methods Uni with State researchers LSU and Arizona collaboration at (in We have developed spectrometry-based mass have We approaches to characterize the structure fibrils, oligomers, amyloid of and monomers. aggrega follow These to tools methods excellent are ing an array of technologies to achieve complete understanding complete of technologies achieve array ing an to of the aggregation reaction. of populations interrogation rapid gentle and allow versity) that CE separates species the mixtureof aggregate within figure). (see detection and shapes, online and sizes of various aggregates scatter laser-light fluorescence, laser-induced with absorbance, further provides anisotropy fluorescence laser-induced and ing, the specific structures of to cytotoxicspecies, theirrelationship of toxicity the mechanism unknown. and are formation, amyloid species Each aggregate formed of the different duringamyloid properties chemical and physical unique canformation have pro technique cannot single A toxicity. for differential viding a basis are utiliz we species;thus, aggregate of the different resolve each tissue deposition of normally soluble proteins are associated with associated deposition are of normally solubletissue proteins Par disease, Alzheimer’s (e.g., amyloid-associated diseases >25 develop Oligomeric that aggregates diabetes). disease, kinson’s been in implicated have during the course formation of amyloid be cytotoxic. to considered and are pathogenesis disease Amyloid heterogenous mixtures are aggregates of oligomers Amyloid disease biology. biology. disease Amyloid Indu Kheterpal, Ph.D. Indu Assistant Professor Protein Structural Laboratory Biology Protein 40

BASIC RESEARCH ExpressionRegulation ofGene Laboratory Associate Professor Associate Ph.D. Salbaum, J. Michael onic epigenome, are the principal target ofmaternal target onic are epigenome, theprincipal diabetes. regulation, ofgene such as theembry properties that system andsuggest to aloss of“regulatoryprecision” in theembryo levels. We expression of gene that posit maternal diabetes leads but alsoleads genes cific increasegeneral variability to ain the levels changes in causes nottheexpression betes only ofspe correlate this for we element: that found variable maternal dia that now suggest we studies haveOur molecular a identified variation. an require elementofbiological nations phenotypes these for of incompletepenetrance,expla and phenotypes of definition matches Such apattern theclassical defect. thebirth with is afflicted ofthelitter milieudiabetic ofthedam, afraction only to despiteous model: in sharing themouse theexposure mother. ofa diabetic obvi offspring most individual is Thisfact not do arise in every such defects as tube neural defects Birth etiology. molecular one may than includemore defects tube neural of diabetes-induced andimplies that across theboard genes thepathology defect allonstratesneural tube that maternal not affect diabetes does are deregulated genes by maternal This diabetes. dem defect tube neural ofthese one-third play defects; in tube neural arole thatto genes are known therearepresent, approximately 400 At embryos. in maternalin their expression diabetes-exposed We thatgenes have are altered more than now identified 2000 in theembryo. expression alters gene duringpregnancy betes is hypothesis thatThe this for maternal research project dia defects. tube of neural on the early developing and system the nervous pathogenesis with afocus embryo, the how maternalstand diabetes affects is goal to Our under defects. severe cause can birth pregnancy development: maternal diabetes embryonic during also affects individual,but diabetescompromise thehealth oftheafflicted 2diabetes severely not only 1andtype type Both worldwide. isDiabetes amajor health concern in theUnited States and expression in the embryo. the in expression andtionship gene diabetes, nutrition, between defects, birth FOCUS: FOCUS: SCIENTIFIC REPORT The goal of this laboratory is to understand therela is to ofthis understand goal The laboratory

2 010–2011 ------by theInstitutes National Health. of Research this in is supported laboratory to the developing embryo. precisionthat is detrimentallatory so regu diabetes leads to thedegraded ofhow maternal thequestion pursue andto etiology defect in birth role genes on their based hypervariable (see figure).a defect We have these begun now to characterize without embryos than patterns variability gene different very show defects tube neural with levels, expression on embryos while indistinguishable based defect: tube aneural without or with pregnancies diabetic from embryos to distinguish tool as adiagnostic used could be variability increased tes. Infact, ofdiabe induction andgenetic chemical both with in models We expression ofgene this have increase in variability observed tube defects cluster together, as shown by the brighter colors. together,brighter by the cluster shown as defects tube yellow higher and blue depicting representing lower than the mean. Embryos with neural with mean, the from deviation of magnitude the signifies intensity color The defect. tube (NTD) (no with NTD) without and pregnancies aneural diabetic from embryos in ability vari expression gene of analysis abioinformatics of representation 2:Figure Graphical bifida. spina with 1:Figure embryo early An (1) Ph.D. Zhang, Xiaoying MacGowan; Jacalyn Team:Research Ph.D. Salbaum, J. Michael Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL - (2) - - BASIC RESEARCH 41 - - 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Faculty: Jeffrey M. Gimble, M.D.,Ph.D. Research Team: Katie Hamel, B.S.; Forum Shah, B.M.E.; Vik Singh; Caasy Thomas-Porch, B.S.; Xiying M.D. Wu, and the LSU Department Mechanical of Engineering, well as the following including centers and international national as Memorial Hopkins, Johns Columbia, Harvard,universities: MIT, and Tufts. The laboratorySloan-Kettering Tulane, Cancer Center, activelyis pursuing the role biological of circadian mechanisms in regulating the metabolism of adipose and tissue bone. These transcriptomics and molecular approachesstudies at involve have The outcomes levels. and tissue cell the stromal/stem for the treat for of chronotherapy the use implications potential metabolic osteoporosis,and related of obesity, ment disorders. National from the laboratory funded is in this grant(s) by Research and Pen the Initiative, Maryland the of Health, Cell Stem Institutes nington Biomedical Research Foundation. Biomedicalnington Research - - The laboratory goal further of this to is the char :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS acterization and understanding and adult of adipose tissue metabolic, for circadian, stem and regenenerative cells medi cal studies. The Stem Cell Biology Cell Laboratory The Stem both a clinical maintains focus its in science and basic efforts. research translational The from adipose cells laboratory’s stromal/stem on work adult andtissue bone marrow tissue-engineering has potential and regenerative medical applications. The laboratory continues characterization, new approaches isolation, the develop to to and cryopreservationusing differentiation, cells of stromal/stem both vitro in models. vivo and in includes collaborations This the LSU School at Medicine of Veterinary with investigators Jeffrey M. Gimble, M.D.,Ph.D. Professor Stem Cell Biology Laboratory Biology Cell Stem 42

BASIC RESEARCH Taste Laboratory Genetics Associate Professor Associate Ph.D. (Smith) Richards, K. Brenda short-chain acyl-CoA dehydrogenase ( acyl-CoA short-chain ofoxidation, inactivation agenetic with using strain amouse acid by fatty signals from are preferences affected how food understanding on is intake.focused alter food Another project acidavailability may offatty over which aspect exists troversy yet con epidemic, to treat targets theobesity pharmacological pathways as acidmetabolic may serve within fatty Enzymes strains, and biological relevance. parental tionalbetween annotation, expression differential gene several on analysis, criteria including bioinformatics based func determined be will contained withinregion therefined QTL mapping interval composite ing panel; data analysis is now waySNP under genotyping us genic–by–recipient F2 cross using custom ahigh-density, mapping genetic in a con fine-structure out we carried the responsible genes, of the QTLthe location and identify cosegregatestraits. with the food-intake activity Chr 17 QTL and surprisingly showed that increased physical associatedwith the effects the phenotypic retained strain ment ofChr 17 (donor) asecond from DNA This congenic strain. (recipient) strain ofone thegenome possessing andasmall seg ofinterest, acongenic wechromosomal region strain developed carbohydrateincreased intake. andtotal calorie To isolate this (Chr) chromosome of mouse 17 that encompasses theQTL for on a region are focused aquantitativeon Current scale. efforts measured associatedDNA aphysical with phenotype, or trait controlsintake.multiple genetic food on AQTL of is aregion intakecalorie in mammals, evidence thusfor providingstrong quantitative loci first trait (QTL)carbohydrate,fat, for and total ences in humans. Toward the identified this aim, ourlaboratory howvariation genetic contributes prefer tous food understand in animals will help phenotypes or traits is these for responsible that variation sequence DNA influential the Finding choices. food various among carbohydrate to they eat choose selecting when in theamount and offat widely Similar mice to vary people, intakes. contributing to macronutrient andtotal energy factors netic FOCUS SCIENTIFIC REPORT : The goal of this laboratory is to identify thege is to ofthis identify goal The laboratory

2 010–2011 . Positional candidate genes Acads ). ). Acads T encodes o resolve resolve o ------associated with the taste of protein alone. taste the protein of with associated C/P or F/P, respectively. Equalizing the protein content in both choices prevents problems bohydrate/protein (C/P) or a fat/protein (F/P) containing mixture 78/22% of energy from tional Institutestional Health. of Research by grants this in the isfrom Na supported laboratory acidoxidation andeating behavior. fatty link between putative the as mechanisms to cellular energy-sensing pointed involved genes on pathwaysstudies in energy-sensing have Follow-up in thebrain. expression gene on diet of ahigh-fat that and CREB signaling in neurons. Together these findings suggest or diet: oxidative mitochondrial phosphorylation, dysfunction, top-scoring pathways significantly most modified by genotype Pathway Ingenuity diet. low-fat Analysis revealed thethree ofAcads+/+ those mirror diet a high-fat mice to-deficient that thetranscriptionalofAcads responses Acads+/+ inof thebrain analysis expression gene ofglobal amicroarray acidoxidation intake, andfat we performed fatty with associated mediators molecular the understand better choice. acids To alters food in theoxidation offatty enzyme toward carbohydrate, that demonstrating theloss ofaspecific consumption food fatdeficient away andmicefrom shift - choice, Acads a dietary offered When acids. fatty short-chain responsible forthe mitochondrial enzyme of beta-oxidation Nutrient intake is assessed in adult mice provided with a choice between two diets: acar diets: two between achoice with mice provided adult in is assessed intake Nutrient Jacob Simon, B.S. Lisa M.S.; DiCarlo, Ginger Robertson, B.S.; Team:Research (Smith) Ph.D. K. Richards, Brenda Faculty: -deficient mice may be protected against the effects againsteffects micethe mayprotected be -deficient Acads PENNINGTON RESEARCH CENTER BIOMEDICAL mice fed either a high-fat or low-fat diet. We diet. low-fat or found mice ahigh-fat either fed control mice a fed Acads-/- and and - - BASIC RESEARCH 43 - - 010–2011 2

Figure 1. Injections of EP-100, a conjugate of LHRH InjectionsFigure 1. of EP-100, and a lytic peptide, decrease tumor weights of PANC- 1 xenografts in nude A further mice. decrease occurs stimulat follicle with pretreated are mice the when weights Tumor are compareding hormone (FSH). a baselineto value obtained necropsy by of a group of tumor bearing prior mice the beginning to of tumor represent baseline the Bars above treatment. growth; bars below the baseline represent tumor vs. baseline). P <0.001 +++, P<0.05; regression (+, SCIENTIFIC REPORT SCIENTIFIC Faculty: William Hansel, Ph.D.; Sita Aggarwal, Ph.D. Research Team: Theodore (Adjunct); Rajasree Ph.D. Gauthier, Solipuram, Ph.D.; Qingxia Mack Ray Wang; all animals (follicle stimulating hormone, FSH) to the culture the culture to hormone, FSH) stimulating (follicle animals all LHRH medium increased expression controls of the gene that expression cells. cancer receptor pancreatic in observationThis carry led to us out experiment an test the to before of the LHRH- pretreatment administration FSH idea that pancreatic bearing human nude in mice lytic peptide (EP-100) tumors the number increase of LHRH cell cancer would recep tors and result in a more a in effectivetors and result the in treatment. shown As to aloneat the be Whencase. administered proved this figure, bearing and 0.2 mice to mg/kg) of twoeach (0.02 doselevels tumors, pancreatic the LHRH-lytic human peptide (EP- PANC-1 caused at decreasesnecropsy. weights in tumor significant 100) caused further with FSH Pretreatment decreases tumor in alone or FSH with saline (vehicle) treatment weights. In contrast, tumor growth. continued allowed These significant results are because effective no really are for cancer treatments pancreatic currently available. laboratory supported from is Esperance in this grants by Research Foundation. Family and Inc., Pennington the Pharmaceuticals, Sita Aggarwal, Ph.D. Instructor The laboratory goal prolong of this to and is :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS improve the quality of life of cancer patients by developing the quality developing patients by of cancer of life improve effective therecurrence and to treatments methodsprevent after cancer of treatments. The efficacy of the LHRH-lytic ison dependent peptide drugs the number of LHRH receptors expressed cell on the cancer membrane. In a laboratory experiment, adding that noticed we reproduction in pituitary control the of one hormones that Current Project Current In previous studies, our team developed a new class of drugs developed studies, of drugs In previous a new class our team (lytic proved that effective peptide conjugates) in targeting and tumors in metastases and in cells destroying cancer human in luteinizing as of a hormone, such These conjugates test mice. lytic a such and peptide, hormone-releasing hormone (LHRH), cancer the molecules on hormone receptor to bind Phor21, as (the guided be missiles to likened membrane. They may cell destroy that lytic peptide) (the warheads bearing hormone) membrane. Thesethe cell drugs effectively andtarget destroy prostate, and breast, pancreatic cancers ovarian, testicular, in the nude mouse model. One of the LHRH-lytic peptides now is trials. 2 clinical being Phase tested in William Hansel, Ph.D. Professor The William Hansel Cancer Prevention Laboratory Prevention Cancer Hansel William The 44

BASIC RESEARCH Ubiquitin BiologyLaboratory Assistant Professor Assistant Beth Floyd, Ph.D. production of other proteins needed for lipid andcarbohydrate for ofother proteins needed production as the“masterprotein that switch” functions in regulating the ubiquitin system enzymes that alter PPARγ stability and activity that alter PPARγ andactivity enzymes ubiquitin system stability asmall of set to identify technology interference RNA on based method anovel we screening developed high-throughput task, To accomplishthat in adipocytes. determine PPARγ this activity the specific components of the ubiquitin-proteasomesystem to has focus identifying now Our turned in adipocytes. activity regulation ofPPAR TZD-dependent for is necessary system ubiquitin that functioning a and system, ubiquitin-proteasome ofPPARgion pathway.some Using we that found approaches, there these peroxisome proliferator-activated receptor gamma (PPAR gamma receptor proliferator-activated peroxisome the on depends ent to obesity. formation ofadipocytes The imbalance consequencesphysiological oftheenergy inher (fat cells) Adipocytes as hypertension. play acentral in the role 2diabetes, andcardiovascular diseases such type syndrome, is associated thedevelopment with ofmetabolic Obesity PPAR PPAR in adipocytes. metabolism have how cellularto used dissect approaches andmolecular PPAR between this connection Tothe ubiquitin-proteasome in system adipocytes. understand show that studies PPAR Our functions. ofproteins, this making pathwaydestruction central to cellular pathway timed highly conserved thecarefully for responsible proteasome ubiquitin-proteasome The system. is system a theubiquitin- called ofenzymes set bydetermined acomplex intracellular ofmost proteins, such stability as PPARThe 2diabetes. utes to type maynew offer insightscontrib intoand stability how obesity PPAR thelink between indication that understanding (TZDs). alter drugs ediones PPAR These thiazolidin the drugs, antidiabetic classof prescribed monly proteasome regulation of protein stability andactivity. proteasome regulation ofprotein stability is influenced by ubiquitin- formation andfunction adipocyte FOCUS SCIENTIFIC REPORT γ �� is oftheubiquitin-protea by recognized components : The goal of this laboratory is to understand how is to ofthis understand goal The laboratory γ responsible for binding TZDs is by recognized the TZDs binding for responsible

2 γ 010–2011 activity and degradation, we degradation, and activity �� γ �� is thecellular ofacom target �� γ γ activity is regulated by is regulated activity activity and stability, an stability, and activity γ activity activity γ

, is is , γ ), a a ), - γ - - - - - Metabolic Syndrome. the Pennington Center Biomedical forResearch Botanicals on and can Association, Diabetes Institutes the National Health, of and Research by grants this in the isfrom Ameri supported laboratory diabetes. 2 such as type disorders and metabolic obesity link between the in understanding is factor proteasome acritical system and strengthen biology the idea that the ubiquitin-adipocyte our findings indicate of component Siah2 as an important Taken ofPPARγ in adipocytes. together,determines theactivity to that theidea Siah2 support lending adipocytes, forming for We that also Siah2 learned is necessary in adipocytes. sensitivity insulin that Siah2 and affects PPARγ with adipocytes, in acts ofPPARγ activation for that by TZDs, Siah2is necessary inter a protein Siah2. called year, Inthepast that we learned Siah2 we identified, are currently centered oftheenzymes one on studies Our uitin ligases that alter PPARγ in adipocytes. stability over screening 225 ubiquitin ligases, 5 weubiq After identified drug development. for targets attractive are particularly enzymes these because theubiquitin on ligases we focused Inourscreen, in adipocytes. Lauren Carter M.S.; Kirk-Ballard, Heather Kilroy,Gail B.S.; Team:Research Floyd, Ph.D. Beth Faculty: extent asextent elimination of PPARγ, the “master switch” for forming adipocytes. elimination of ubiquitin ligase Siah2 reduced formed to the number of adipocytes the same In contrast, development. adipocyte on effect no (NT) had protein known any target not does siRNA that of dye. ared with Introduction were stained adipocytes formed fully ment, to determine whether a specificprotein is required for Informing thisadipocytes. experi used be can protein a“targeted” of RNA (siRNA) production stops interfering that Small Control siRNA PPAR PENNINGTON RESEARCH CENTER BIOMEDICAL γ siRNA Siah2 NT - - - - Basic Research 45

2010–2011

Clinical Research, and and Research, Clinical Science. Population nineteen has The Center laboratories Service Core million $20 and over Opened in 1988, Opened in 1988, Biomedical Pennington laboratories 52 now houses Center’s span the that three programs Research, - Basic in technologically equipment. advanced SCIENTIFIC REPORT SCIENTIFIC PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON CLINICAL RESEARCH

46 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER At Pennington Biomedical Research Center, Clinical Research is Since the inception of our first clinical studies in 1992, the a vital component of our enterprise. Our strategy to produce ex- strong infrastructure outlined above has resulted in an enviable cellence in research results begins with support from the incep- record of completed research. As shown in the figure, this ap- tion of the research question and continues through support for proach has resulted in more than 18,000 patients being enrolled the analysis of results and publication. This is not a do-it-your- in more than 400 studies. self model. In the 19 years since our first clinical study, we’ve developed a systematic approach to Clinical Research support. Two major shifts in direction We provide peer-review services for every study proposal by are upcoming for our Clinical assigning anonymous reviewers. Our Institutional Review Board Research operations. While we provides online templates and processes research protocols in a are now established in adult timely manner. The Scheduling Committee assures that funding research operations in a new CLINICAL RESEARCH is in place for all of the 11 Core service providers and schedules Clinical Research Building, reno- the study so that we can maximize efficiency of our Inpatient vations are under way for a new and Outpatient Units. Our clinic assigns a study coordina- outpatient pediatrics wing. We tor, and our Clinical Biostatistics and Data Management Core plan to use this new wing to ini- and Recruitment Core are engaged early in the launch of the tiate a programmatic approach study. We provide ongoing monitoring via our Record Library to childhood obesity. Another new direction will be reflected Donna Ryan, M.D. services. There are intranet applications to allow investigators Associate Executive in closer ties to the Louisiana to track participants and their data in real-time from investiga- Director for Clinical tors’ desktop computers. Our Biostatistics and Data Manage- State University School of Research ment Core is available to assist with analyses. There are online Medicine in New Orleans, as applications available to assure that all investigators are up to we build collaborative relation- date for training in privacy and HIPAA compliance issues, in ships and focus ever more on a Good Clinical Practices, and in Human Subjects Protections. The broader range of disease states overall approach at Pennington Biomedical is one of systematic on which to focus our research and uniform processes and a commitment to the highest quality efforts. possible in the conduct of Clinical Research.

# Telephone # Screening # Enrolled Studies Year Contacts Visits % Females % Minority in Studies % Females % Minority Started 1992 1962 771 67% 13% 255 61% 15% 5 1993 2543 1124 59% 20% 575 58% 20% 14 1994 3742 1423 49% 37% 451 57% 24% 15 1995 6020 2005 49% 52% 574 48% 22% 7 1996 4799 1432 54% 25% 628 44% 19% 13 1997 4261 1667 63% 32% 612 56% 23% 11 1998 5282 2218 68% 33% 783 73% 22% 25 1999 4537 1686 61% 26% 618 58% 22% 13 2000 5458 1933 76% 32% 731 69% 24% 24 2001 4432 1707 76% 39% 804 77% 37% 26 2002 4388 1836 69% 35% 999 69% 36% 24 2003 6224 2391 71% 41% 1394 72% 37% 29 2004 6741 2502 67% 44% 1429 66% 38% 36 2005 8576 3775 57% 39% 1723 64% 35% 16 2006 6984 2213 67% 39% 1189 68% 36% 35 2007 7501 1974 67% 42% 1011 67% 35% 32 2008 16876 2199 68% 43% 1556 66% 38% 30 2009 16490 1934 64% 28% 1011 59% 23% 25 2010 13032 2483 58% 27% 1790 58% 22% 39 TOTAL 129,848 37,273 18,133 419

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 47 48

CLINICAL RESEARCH Behavior Technology EatingandObesity Disorders Laboratory: Assistant Professor Assistant Ph.D. M. Stewart, Tiffany Department of Defense. ofDefense. Department an ongoing, ten-year collaborativePBRCbetween and the effort at PBRC(H.E.A.L.T.H.) was developed andrepresents program Healthythe Eating, Activity, and Training Lifestyle Headquarters of theWeight Project, Measurements and Standards Soldiers for Weight Measurements and Standards for Soldiers. behaviors. (e.g.based Smartphones) interventions changes in for health clinic-based, and internet/technologically advanced mobile- This of researchcommunity-based, involves testing theefficacy image disturbance.ment obesity, ofeating andbody disorders, theassessment, for prevention,behavioral approaches andtreat BehaviorThe Technology research conducts on Laboratory the Female Athlete Triad (i.e., low energy availability/disordered dangerous in increases female it riskpecially athletes for because eatingdisordered female athletes among is prevalent, andis es that Research suggests risk factors. EDin reducing andobesity prevention in the modifications of onset ED and small lifestyle theuse ofa supports of EDs has considerable health public significance.Research prevention disorders, associated these with andmortality ity cost oftreating eating (EDs) and disorders thesubstantial morbid Athletes. Female in Intervention Weight Healthy fighting advanced force.technologically andimprove combat readiness our for oftheMilitary all branches to result to in dissemination program oftheH.E.A.L.T.H. expected tested in theLouisiana Army National Guard(LANG). England (Ft. andNew Reserves), NC, Bragg, and is being projects pilot andtestedin deployed two lation has health been program, iswherever considered they apopu are H.E.A.L.T.H. in the world. use it can members andtheir family Soldiers Smartphone) so (e.g. andis “portable” technology interactive Internet, ting edge ing overall incorporates cut H.E.A.L.T.H. healthgoals. and fitness in reach to programming includes members aid Soldier’s family also H.E.A.L.T.H. combat readiness, andWarfighterperformance. in maintaining healthyaid Soldiers status, weight fitness status, and body image disturbance on health behaviors. image health disturbance on behaviors. and body weight eating for vanced programs disorders, management, FOCUS: To ad investigate oftechnologically FOCUS: theimpact SCIENTIFIC REPORT Healthy Weight Tiffany Stewart, Ph.D. Stewart, Tiffany Faculty: The H.E.A.L.T.H. program ist designed program H.E.A.L.T.H. The

2 010–2011 (HW) program targeting targeting program (HW) This study is This study Given the As a part apart As - - o - - - - - Ventures. Defense, of andThemelios Department Health,of the U.S. Research in this laboratory is funded by the National Institutes prevention students. to populations, e.g. large scale university Fraternity, Delta Delta tocluding Delta with deliver eating disorder in ofpartnerships is in anumber BE currently engaged platform. Emer.ge reside on adherence. programs These environment to network reinforcea social learning andpromote andassessment programs toolsment. are The integrated within image, eatingandweightincluding body disorders, manage apps)phone, ipad ofhealth that range behaviors awide address (e.g. applications, andhealthmedia e-games Internet, Smart i.e. including interactive, learning programs experiences, digital offlineapproaches into online/digital evidence-based adapts BE by andventure angel investors. at funded discovery capital PBRC) and is an entrepreneurial venture (formed as aresult ofscientific health behavior technology to commercialize evidence-based Body Evolution Technologies (BE). collegiate 500 intervention among female athletes in foursites. of HW the controlled trial torandomized theeffectiveness test osteoporosis) andsubsequent injury. is acluster This study eating, menstrual disorders, and decreased mineral bone density/ to this work. this to dedication their Ph.D. for Williamson, &Donald M.D. Ryan, Donna Acknowledgements: Walker, B.S. Zeno, Jonathon Verdis M.S., Walden, Heather Tavernit, Sarah B.A., Switzer, B.A., RD, Michael LDN, M.S., Ragusa, Shelly B.S., Paul Mounts, Stat., App. M.S. Han, Hongmei, M.A., Hall, Lindsay M.A., Davis, Allison B.S., Bouillion, Jeremy Ph.D., Ray Allen, M.S., Acharya, Archana, Team:Research PENNINGTON RESEARCH CENTER BIOMEDICAL LANG H.E.A.L.T.H. and Body Evolution Technology Applications Technology Evolution Body and H.E.A.L.T.H. LANG BE is acompanyBE formed , an e-health, online online e-health, an , - - - CLINICAL RESEARCH 49 - - - - 010–2011 2

Faculty: Ph.D.; Brantley, J. Phillip Ph.D.; Champagne, Catherine David Harsha,Ph.D.; ValerieH. Myers, Ph.D. Research Team: Patti Katherine Boyd; Cash; Erma Levy; Megan McVay, M.A.; Jose Silgado, M.A. SCIENTIFIC REPORT SCIENTIFIC Valerie H. Myers, Ph.D. Instructor Figure 1. PharmacyFigure 1. Costs its association with cardiovascular disease and diabetes, obe and diabetes, its disease with cardiovascular association sity’s economic burden to individuals and societysity’s individuals economicburden to enormous. is Members Office of our laboratory with the Louisiana collaborate to the state-managedof Group Benefitsinsurer, (OGB), health study the feasibility and effectiveness of medicalproviding obese severely to treatments adults.and surgical In onestudy, obeseseverely members 40 and randomly volunteered, were selected surgery loss for insurance-covered weight (WLS). Several studied, medical were total in the change including outcomes and pharmacy expenditures and the subcategories of medi cal and pharmacy expenditures obese in following individuals compared theseWLS. also costs expenditures to We severely in non-pharmacy Total obese members receiving not WLS (n=911). medical for WLS costs lower non-WLS were patients comparedto patients beginning post-surgery four years and lasting through pharmacy for WLS costs lower were Total post-surgery. six years participants but these two lower at and three post-surgery, years costs remained However, figure). (see costs maintained not were agents, agents, for antidiabetic and dys antihypertensive lower lipidemic agents through all six post-surgery years under study. six post-surgery agentslipidemic all through under years study. laboratory supported Na from is the in this grants by Research the through of Louisiana of and Health State the tional Institutes Group of Office Benefits. ------David Harsha, Ph.D. David Associate Professor The Weight Loss Mainte Loss The Weight Catherine Champagne, Ph.D. Champagne, Catherine Associate Professor The goal of this laboratory is to investigate weight weight The laboratory goal investigate of this to is :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS ment and examine and impactment their on biomarkers and health outcomes. loss techniques that promote long-term manage promote that weight techniques loss Weight Loss Maintenance Trial: Maintenance Loss Weight Adults: Obese Severely of Management Weight and obesity of overweight rates rank Louisiana’s Along with among States. the highest the United in nance (WLM) trial was a three-phase, multicenter randomized (WLM) a three-phase, was trial nance multicenter long- strategies for maintaining alternative comparing trial loss. Our laboratory weight term sites one was of four clinical eligible if were they were Persons participating the study. in and on medicationsoverweight/obese for hypertension and/or program. loss Dur weight a six-month I was dyslipidemia. Phase II, participantsing Phase random I were who Phase in lost > 4 kg (IT) Internet-based conditions: one of threeized maintenance to personal monthly treatment, contact or a self-directed (PC), (SD) III, participants SD In Phase months. for 30 condition con control tinued without further intervention, while PC participants were contin or (PC-Control) re-randomized further no to intervention Results (n=489). more for months 30 ued (PC-Active) intervention beenpublished. I, During mean from I and II have Phase Phases During kg. the next II), (Phase months 30 7.9 was loss weight difference adjusted (mean SD PC less than in was regain weight Over the next 30 -0.43). to -2.8 CI 95% kg; of -1.6 months 30 at PC-Active in kg 1.0 increased by weight mean III), (Phase months difference adjusted (mean 0.5 PC-Control in and by kg Mean weight p = 0.54). 2.7; to -1.4 CI 95% of 0.6 kg; those in originally kg -3.2 66 was at change months difference (mean SD in kg PC and -1.6 to assigned 77% Overall, p = 0.04). -0.1; to -3.0 CI 95% kg; of -1.6 PC remained below to assigned of those originally = 0.002). (p SD in entry 63% compared to weight These suggestafter 30 months, findings that initial an provided intervention of a behavioral no continuation the nextover benefitadditional weight on 30 months. after the majority 5 years, of individuals However, a weight maintained lostwho successfully weight level. below initial their Phillip Ph.D. Brantley, J. Professor Behavioral Medicine Laboratory Behavioral 50

CLINICAL RESEARCH Behavior Modification Clinical Trials Boyd Professor Boyd Bray DSE (p <0.001). had ofILI participants Agreater proportion in ILI increased by 20.9% versus fitness Mean years. 5.8% in < 0.001) at year 1. three over somewhat This thenext faded (DSE) andeducation group (p0.7% in thediabetes support intervention (ILI)the intensive group was versus 8.6% lifestyle incidence major for cardiovascular weight events. The loss of ofan intensive weightfects loss intervention thetimeto on 2diabetes evaluated ef thelong-term type with patients 16-center clinical randomized trial in overweight and obese Trial Diabetes) in Health for (Action AHEAD Look way. under This ofthetrial has phase gotten just in thevarious treatment groups by using accelerometry.activity toPennington thelevel examine of was funded Biomedical, this for trial, study involvingoutcomes An ofdiabetes. ancillary to 2014 refunded trialbeen benefited).The has eryone to follow group (i.e., ev to oftheinitial those and corresponded lifestyle outcomes study, theconversion rates were similar in all groups intervention. Fromioral thetimetrial was converted to an behav extra group getting andtheintensiveformin lifestyle remaining treatment on met with metformin taking people was converted into with outcomes-intervention amixed study were results these announced, thetrial weight2.5% After loss. rate to diabetes by 31% asmall significant andproduced but slowed theconversionacross several ethnic Metformin groups. 58%; were responses similar andwomen, men as well among as weight theincidence ofdiabetes andreduced byof their body an averageto lost theinitial of7% program intensive lifestyle during randomized the phase, double-blind, those assigned 13th has now completed its study year. Intheinitial years 3.2 total of22 centers across theUnited States are involved. This glucose impaired with tolerance andahigh risk A ofdiabetes. andwomension men to diabetes in ofover apopulation 3,000 therate on placebo or ofconver versus metformin program randomized trialdouble-blind, compared an intensive lifestyle Diabetes Prevention Program Outcomes Study: effects of points. weightloss on end multiple effects to achievemodification weightloss and the evaluation of the FOCUS SCIENTIFIC REPORT : This unit focuses on behavior, Thison unit focuses andactivity diet, Professor Champagne

2 010–2011 Instructor Myers This This Professor Ryan : This This : - - - - - Institutes Health. of Research by thisgrants in is funded the from laboratory National remains to determined. be acuity in themental this whether is also reflected improved mobility; It is have anticipated who weight that lost people will have andmovement abilities oftheparticipants. ing thememory involving thePennington Center in 2009. was Itis funded test through 2013. refunding totrial has its led study An ancillary < 0.001) 7.3% versus from to 7.2% success in DSE. The ofthe MeanHgbA1cmedicines. 7.3% from dropped to 6.6% in ILI (p inreductions diabetes, hypertension, and lipid-lowering Erma Levy, R.D.; Barbara Cerniauskas, R.D.; Michelle Begnaud, R.D. Begnaud, Michelle R.D.; Cerniauskas, Barbara Levy, R.D.; Erma R.N.; Arceneaux, Jennifer R.N.; Dragg, Amber Strate, R.N.; Allison Team:Research McIlhenny Endowed Chair in Health Wisdom Professor Professor Greenway,Frank L, M.D., M.D., Ph.D., Ph.D., George A. Bray, A. M.D., George Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL Professor, Clinical Research for Director Associate Executive Professor Greenway Professor ; Timothy Church, M.D., M.P.H., Ph.D., M.D., Church, ; Timothy ; Valerie Myers, Ph.D., Ph.D., Myers, ; Valerie Boyd Professor Boyd Associate Professor Associate Gupta ; Alok Gupta, M.D., M.D., Gupta, ; Alok ; Catherine Champagne, Champagne, ; Catherine Instructor Professor, S. John ; Donna H. Ryan, Ryan, H. ; Donna Professor Church Associate ; - CLINICAL RESEARCH 51 - - - - P- - 31 - - 010–2011 2

Faculty: FACSM, Ph.D., Earnest, Conrad Timothy Church, M.D., Ph.D.; M.P.H., Ph.D. Johannsen, Neil Research Team: Melissa Lupo, B.S. SCIENTIFIC REPORT SCIENTIFIC tive, amateur cyclists and triathletes (see figure). cyclists figure). (see amateur triathletes and tive, of antioxidants Astaxanthin, levels high has which and the ability fat metabolism increase mice, in to a phytochemicalis best known for its a feed as use for farm-raisedadditive salmon, giving the fish their pink flesh, butisalso abundant in crawfish. One on studies focusing the of our current now is extracts green of black and examination tea on performance.exercise exhibit and green Black tea andantioxidant anti-inflammatory properties been some in studies,that, indepen shown have dently to improve exercise recovery. Though black exercise recovery. improve dently to of action, mechanisms similar have and green tea characteristics be unique may that have also they The goalcomplementary. of the TEA to is Study affects combination examine this how exercise that damage and muscle performance, recovery, bouts intense of exercise. accompany may laboratory funded NIH, is in the this by Research Health. and Kemin Institute, SportsGatorade Science Neil Johannsen, Ph.D. Instructor ued spectroscopy exploration resonance magnetic of using ( MRS) to exploreMRS) The to examina energy muscle skeletal pathways. important is involved those individuals tion of these pathways to aging- various sportin tied to is and health and pursuing overall and mortality-related processes. disease to One is of our goals the necessarybuild validate and examine various equipment to performed of work for the amount accounting by groups muscle task, work muscular a given in along with a more repeatable and perform to way valid these assessments. Nutrition Supplementation: discovered we study, In a recent compound supplementation known with a carotenoid that as astaxanthin increased cycling performance in local, competi covery. The results of this pilot study pilot The interval results of this demonstrated that covery. severaltraining improve parameters may resis insulin related to 30 seconds to ~2-minutes, followed by an equal an period by followed of re ~2-minutes, seconds to 30 and metabolictance syndrome better recom traditional than mendations. Interestingly, the interval included group training no Interestingly, mendations. training of the traditional dropouts ~20% while from study, the droppedgroup out. the contin is of our lab interest A current Technologies: New - - - - TimothyChurch, M.D., M.P.H., Ph.D. John S. McIlhenny Endowed Professor, Wisdom Health in Chair During the past 2 years, we have explored During the past have we 2 years, The laboratory goal understand of this to is the :

Data show that cyclists supplementing with astaxanthin improved their cycling performance during during performance cycling their improved astaxanthin with supplementing cyclists that show Data km timea 20 trial an by average of two minutes. By comparison, ingesting traditional carbohydrate typically improves 40 km time trial performance 40 to seconds. 30 by

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS effects nutritionandand on of metabolism, obesity, exercise performance physical to and health. relate they fatigue as The Exercise Biology Laboratory the Exercise comprises Testing Program. The Exercise Testing and Wellness Center, Fitness Core, provides assessments reliable of physiologic, cardiorespi Core ratory, and muscular strength parameters related to exercise strength exercise muscular and parameters to related ratory, moni exercise performance. provides accurate The Center Fitness the use of intervalthe use vs. steady-state training traditional aerobic (NIH)-funded of Health Institutes a National through training lasting exercise periods of intense Intervalgrant. involves training Exercise Training: toring for studiestoring requiringand specific interventions, exercise Biomedical help Pennington to place in Program is the Wellness lifestyles. adoptemployees healthy The Exercise Biology Labora tory tasked with exploring also is of improving means various performanceexercise new technolo training, exercise through supplementation. nutrition and gies, Conrad Earnest, Ph.D., FACSM Associate Professor Exercise Biology Laboratory Biology Exercise 52

CLINICAL RESEARCH Human Physiology Laboratory in Diabetes and Metabolism Chair Gordon L. Professor,Douglas Eric Ravussin, Ph.D. (CT) techniques. (CT) emission positron or tomography (PET)/computed tomography thermography using infrared either mass on and activity mones andthyroid hor ofcold overfeeding, exposure, the impact balanceenergy in adult humans. at We looking are therefore tissueadipose may in theregulation role of have an important Recently, fourmajor have manuscripts that suggested brown determinants. molecular its and insulin sensitivity ofweight gain thedeterioration on of at to theimpacts look We up theresults thedata andwriting are analyzing pocytes. adi hyperplasic or hypertrophic had similar but fat total body who in 35 study lean andoverweight participants overfeeding completed an just eight-week 40% Physiology Laboratory regulators of adipocyte hypertrophy/hyperplasty. The Human are important to differentiate – andtheir capacity adipocytes to –preadipocytes precursors that theavailabilityofadipocyte tissue Moreover, adipose tocutaneous expand. we hypothesize ofthesub is associated theinability with obesity hypertrophic sites,pic whichin results insulin resistance. We hypothesize that andecto leads in offat visceral to deposition depots increased tissue adipose totaneous accommodate fat excessive dietary Itis ofthesubcu abnormalities. hypothesized that theinability Fat metabolic with individuals. is gain associated among varies andhyperplasty hypertrophy ofadipocyte obesity. degree The contribute to theaccumulationperplasty) tissue ofadipose in size (hy andnumber (hypertrophy) Increases in adipocyte restriction in humans nonobese restriction on biomarkers of longevity. ofcaloric research to in aging designed assess theimpact andto perform masstions andactivity; adipose brown on ofenvironmental and/orimpact interven pharmacological to muscles; investigate skeletal in the mechanisms molecular cellular and on emphasis with abnormalities, metabolic and tissue in adipose obesity expansion relationships between FOCUS SCIENTIFIC REPORT :

The goal of this laboratory is to ofthis goal The laboratory

Assistant Professor Assistant Yourka Tchoukalova, M.D., Ph.D.

2 010–2011

understand theunderstand Instructor Ph.D. Johannsen, L. Darcy - - -

- - - - from pharmaceuticalfrom nutrition and companies. Institutesal institutional Health, of an grant, private and contracts Research by grants this in the isfrom Nation supported laboratory toin intervention. response pharmacological by deuterium enrichment in DNA andadipocytes preadipocytes recently R01of awarded a5-year thekinetics grant to study vs. peripheral obesity.sity Furthermore, Dr. Tchoukalova was continue in central tissue obe to adipose on focus kinetics by Dr. ofanewgroupestablishment led Tchoukalova will who An exciting development oftheHuman is Physiology the Lab March in 2012. intervention the finished just are of24 followed over aperiod participant last months. Our bothgreatertreatment in scientific armsinterest. Participants theintervention of receiving ofsubjects thenumber maximize tion ratio in favor oftheCR to in intervention order is applied the CR libitum intervention an or ad assigned to either atotalwith enrollment of225 participants multicenter, controlled trial (RCT) randomized parallel-group, University, Tufts University, andDuke is University. a study The ofCR in collaboration Washington with anewstudy conducting (CR) humans, we restriction in are nonobese nowof caloric toandsafety designed assess thefeasibility (CALERIE) study sessment ofLong-Term IntakeReducing of of Energy Effects phase of the As Comprehensive completing thefirst After tissues. these ofhypoxia in impacts andmolecular thephysiological to study techniques liver. have studies state-of-the-art These combined tissue, and muscle,adipose skeletal in insulin on ia sensitivity ofhypox Another endeavor oftheimpact in ourlab is thestudy Instructor Ph.D. Bajpeyi, Sudip PENNINGTON RESEARCH CENTER BIOMEDICAL Blaine Masinter, B.S. Masinter,Blaine B.S. B.S.; Lagrange, Jamie B.S., Gaubert, Mindy M.S.; M.S., Zhang, Zhengyu B.A.; B.S., D. Jeffrey Covington, Team:Research Ph.D. Ph.D.; Sudip Bajpeyi, Johannsen, L. Darcy Ph.D.; Tchoukalova, M.D., Yourka Ph.D.; Ravussin, Eric Faculty: control group. A2:1 alloca - - - - - CLINICAL RESEARCH 53 - - 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Faculty: Marc Hamilton, Ph.D.; Robert L. Newton, Ph.D.; Zderic, Ph.D. W. Ted Research Team: B.S.N., M.S. Hamilton, D.G. Electromyography signals originate (EMG) from skeletal muscles that are used when not sitting and are analogous the electrocardiography to signal for the heart. (EKG) As shown, human leg muscles specialized for standing and light activity are immediately “turned off” when sitting but are capable of working for many more hours per day even when people exercise. to exerting not themselves are cal used for treating atherogenic dyslipidemia depends on the the on depends dyslipidemia atherogenic treating for used cal inactivity. a These of physical to leading are amount concepts paradigm shift experts public for health how recommend physi cal activity prevention. disease for chronic laboratory supported U.S. is from the in this grants by Research the of Health, Department National the Institutes of Agriculture, G. Heart Schlieder American Coca-Cola, and Edward the Association, Foundation. Educational we found that the amount of time spent sitting of time spent the amount hazardous was found that we for metabolic processes whether or people not have exercised. We learned the effectiveness that even common of a pharmaceuti ------Ted W. Zderic, W. Ph.D. Ted Instructor Robert L. Newton, Ph.D. Assistant Professor The goal of this laboratory is to eliminate the disease disease the The laboratory goal eliminate of this to is :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS processes caused by sedentary living. sedentary living. by caused processes Experimental studies subjects human in and laboratory animals be very to in the proving are clinical trials guiding efficient for most effectivethe that direction. revealed team by our Research physiological effectssitting of largelytooindependent are much of body effects and significant weight, meta relevant clinically on ing enough.” This area has promise for reversing the trends for the trends for promise reversing has area This ing enough.” inac physical metabolicchronic caused excessive by conditions ing the metabolic sitting by processes regulated and very light have inactivity. We comparedcontractions to muscles skeletal in focused on mediating the the processes muscle skeletal in heavily inactiv during physical and glucose lipids metabolism of plasma pears better out spread low-intensity to contractions throughout the day. of inactivity day just a single within evident are bolic outcomes Oneintervention. most of the important from concepts the basic inactivity physical studiesscience that is the biological not is equivalent little of too two trials, And in human exercise. recent Inactivity of medical emerging an new area research, physiology, of thinking about way the medical of consequences a novel is and the solutions for a “sedentary lifestyle.” The often work distinct is exercis fromfocuses much” too “sitting on how “not The research in this laboratory involves both basic and clinical and clinical both laboratory basic The this in research involves been identifying have We regard molecularscience. mechanisms tivity. In the Inactivitytivity. translating are we Laboratory, Physiology to mostin order findings efficiently identify research our basic the lifestyle hazards can best the health caused that changes prevent inactivityby schools, One workplace, the venues. in other and of recom exercise traditional to contrast in that is the new concepts it require taking for exercise, that time outmendations of the day sedentary be simply replace possible to may time with very light excit is This activityphysical the normal day. without interrupting beyond be the expecteding because improved may health the in majority of people our society in who do exercise. not These studiesity. forunderstanding insights offer exercise why the perfect not is inactivity, but it physical ap rather to antidote Marc Hamilton, Ph.D. Professor Inactivity Physiology Laboratory InactivityPhysiology 54

CLINICAL RESEARCH Ingestive Behavior Laboratory Associate Professor Associate Ph.D. Martin, K. Corby is (see thenutilized to figure). guidetreatment delivery Most adherence, which dietary to graphs are used quantify The diet. of weight they over will lose they to timeif adhere aprescribed to theamount create that patients individual graphs for depict are used models These change duringunder- and overfeeding. an individual’sematical that models accurately weight predict State University, the develop IBL helped and validate math acollaboration Ph.D.,Through Diana with Thomas, at Montclair weight. intake, andbody food expenditure, energy interventions andpharmacological on ofbehavioral (lifestyle) cacy near real timewhile theytheeffi live also IBL tests at home. The in which remotely intake measuresMethod, ofpeople thefood Remoteand validated Photography Food the smartphone-based conditions. and For free-living ratory example, the IBL developed inlabo both (activity) intake expenditure ment andenergy offood (IBL) specializesin themeasure Behavior Laboratory Ingestive The dietary adherence. graph. When patients are non-adherent, intervention strategies are used to promote the on depicted zone the is in weight body their when adherent considered are Patients delivery. treatment guide and adherence dietary isto used quantify graph loss A weight activity levels, andpromote weight management. activity of interventionsreducefood intake, to increase the efficacy andto develop andtest intakeregulation andactivity offood FOCUS SCIENTIFIC REPORT :

The goal of this laboratory is to understand the is to ofthis understand goal The laboratory

2 010–2011

- - - - U.S. Department of Agriculture of through contracts and Department withU.S. industry. Research by this grants in isthe funded and the from laboratory NIH Baton Rouge. close collaboration with colleagues Woman’s from Hospital in randomizedfunded controlled trial, which will conducted in be NIH- of an five part ventionas years testedover will be thenext inter ofthe SmartMoms Pennington efficacy The Biomedical. in collaboration Dr. with andLeanne Thomas Ph.D., Redman, of was andit developed SmartMoms, is Thislines. called approach national weightpregnant women meet gestational gain guide the technology. Additionally, was adapted theapproach to help dissemination commercialization of andlarge-scale to explore vention weight to promote loss long-term maintenance and Plans inter oftheSmartLoss way are under to theability test intervention. with the SmartLoss satisfied were very ofparticipants ratings showed that majority thevast satisfaction waist circumference to compared thecontrol group. Further, user- pressure and improvements in blood Loss group also experienced (~9% and ~1% weight, ofinitial respectively).Smart body The in thehealth informationweight control than group participants more group lost in theSmartLoss months, three Over participants SmartLoss intervention to track adherence. weight graphs werein the used previously described The phone. smart via to participants data these on based recommendations to homes clinicians, thensent treatment who participants’ from weight,tive exercise, intake body data were andfood transmitted not visit thecenter treatment. for group, IntheSmartLoss objec while they lived at home; they did information smartphone via control group. groupstreatment Both received health or advice healthLoss information intervention an or attention-matched to were theSmart weight randomized either adults and obese IBL’s Inthis controlled trial, randomized previous work. over the weight which built upon SmartLoss, loss intervention called e-health of an and testedtheefficacy recentlythe IBL developed by theNational Institutes funded ofHealth (NIH), astudy During recommendations. gain to national weight helppregnant women meet gestational recently, andvalidated amathematical was developed model L.D.N., R.D.; Desti Shepard, M.A.; Heather Walden, M.S. M.S. Walden, Heather M.A.; Shepard, Desti R.D.; L.D.N., M.S., Ragusa, Shelly R.D.; L.D.N., Pennington, Gina Miller, Ana M.A.; M.A.; Hall, Lindsay M.S.; Davis, Allison Team:Research Ph.D. Martin, K. Corby Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL ------CLINICAL RESEARCH 55 - - - - 010–2011 2

Faculty: Sudip Bajpeyi, Darcy Ph.D.; Johannsen, Ph.D.; Robert Noland, Eric Ph.D.; Ravussin, Ph.D. Research Team: Charmaine Virgile Ph.D.; Tam, Lecoultre, JenniferPh.D.; Zhang, M.S., M.S.; JeffreyD. Covington, B.S., B.A, B.A.; Zhengyu Zhang, M.S., M.S.; Mindy Gaubert, B.S.; Jamie Lagrange, B.S.; Blaine Masinter, B.S. , Ph.D. SCIENTIFIC REPORT SCIENTIFIC Robert Noland Assistant Professor Representative image of lipid (green) and fiber type (red) staining in myotubes (left) and (left) and myotubes in staining (red) fiber and type (green) lipid of image Representative skeletal muscle tissue (right). adipocytes. and study This past the within completed was year, with collaborated process. in for publication We is analysis data and Bret Goodpaster Italy) (University (Ancona, Drs. of Cinti Saverio Pittsburgh). mitochondrial defects muscle Skeletal of level ACTIV. and a high IMCL are often associated with resistance insulin in sedentary obese typeand/or (T2DM) patients. mellitus 2 diabetes The purpose of the ACTIV mitochondrial capacity and contrast study compare to was spectroscopy resonance magnetic by IMCL and [MRS]) (measured from sedentary in with biopsy) muscle controls (measured healthy a family history or without obese in of diabetes, (FH-) subjects, (FH+) subjectsin also with T2DM, trained We endurance athletes. and in weeks) (~3 effects the investigated training short-term of exercise on skeletal muscle oxidative capacity, sensitivity, and insulin IMCL capacity and low people was In T2DM, mitochondrial with content. action. (in myotubes with in insulin Lipid measured correlated not directly measured with IMCL associated not was fromvitro) skeletal retained important myotubes clini However, vivo). (in tissue muscle sensitivity, function, insulin phenotypescal mitochondrial as such (see figure). fitness and physical Robert studies on the roleDr. of nutrition in Noland initiate will production muscle the skeletal of acylcarnitines its and impact on resistance. insulin Metabolomics profiling analyses revealed that models resistance of diminished insulin display free carnitine levels acylcarnitine in Dietary elevations with concomitant accumulation. whole-body homeo improved glucose supplementation carnitine stasis function and mitochondrial models of and human animal in impaired and these tolerance, glucose improvements are associ acylcarnitines. of excess with increased elimination ated Current studies directed are establishing the direct toward mechanisms adaptations. beneficial these for responsible supported National lab is from the Institutes in this grants by Research pharmaceuti funding, from various institutional of and Health, grants cal corporations. corporations. cal ------Darcy L. Johannsen, Ph.D. Assistant Professor Oxidative The purpose protocol of this Sudip Bajpeyi, Ph.D. Instructor

The laboratory goal understand of this to is and charac : Metabolic flexibilityto oxidation theabilityfuelis match to

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS tance, typetance, and aging. mellitus, 2 diabetes terize the molecular mechanism(s) of mitochondrial dysfunction the molecular terize mechanism(s) resis insulin obesity, to its and muscle skeletal in relationship ibility. ibility. Skeletal muscleavailability. metabolic inflexibilityhas been impli be because resistance of a mismatch the origin in of insulin cated muscle increased to leading oxidation and tween availability lipid metabolic This inflexibility accumulation. lipid is proposedlipid to be of impaired density mitochondrial muscle a consequence to functionand/or in insulin-resistant individuals. The influence of morebe may lipid density metabolic flexibility on to mitochondrial conducted a We increased. is demand oxidative lipid when evident study the reliance on fat investigate to oxidation in individuals with In response to capacity. mitochondrial muscle skeletal and low high prolonged moderate-intensity, a during demand same energy the the same was the on fat in oxidation the reliance session, exercise be not may two accumulation lipid muscle groups, suggesting that capacity. mitochondrial muscle by determined cell size Fat and overfeeding. EAT: characterize to was the morphological and metabolic characteris ectopictics predisposing to of both adipose tissues and muscle fat before resistance and afterdeposition weeks and insulin eight of overfeeding that overfeeding. is will hypothesis The overarching increase ectopicsignificantly resistance depositionandinsulin fat and decrease muscle oxidative capacity in individuals with hyper trophic adipocytes with hyperplasic individuals more in so than Over the past few years, our group has conducted cellular, molecu Over the past conducted has our group years, few cellular, lar, and functional studies samples muscle skeletal in obtainedlar, from FLEX, BARIA, and PRISM, EAT, studies: MITO, clinical the following the metabolic, assess to a wide of assays use CALERIE array I. We and physiological characteristicsmitochondrial, muscle. of skeletal function.muscle skeletal on Effectsaging of MITO: capacity been decrease has muscle skeletal with in to age shown of intramyocellular increased accumulation to be related and may mitochon fewer ceramides), (DAG, metabolites fat or (IMCL) lipid type of oxidative loss dria, and a preferential fibers. 1 muscle The purpose study of the MITO further these to was investigate relationships in elderly and young individuals. immunohisto Using chemistryin elderly higher IMCL found significantly we techniques, compared with young individuals but no difference in thenumber of type 1 or type fibers. 2 muscle flex density metabolic on mitochondrial muscle of Role FLEX: in Diabetes and Metabolism and Diabetes in Eric Ravussin, Ph.D. Ravussin, Eric DouglasProfessor, L. Gordon Chair John S. McIlhenny Skeletal Muscle Physiology Laboratory I Muscle Physiology Skeletal JohnMcIlhenny S. 56

CLINICAL RESEARCH John S.McIlhenny Skeletal MusclePhysiology II Laboratory Skeletal Muscle Physiology Assistant Professor, of Director Ph.D. Civitarese, E. Anthony to caloric restriction (CR). restriction to caloric (KO) Knockout ofPARL protein in the thought genes toprincipal response(s) mediate themolecular esis andupregulate theprotein content ofSIRT1, of the one myotubes to was able induce mitochondrial biogen primary damage to Conversely, mitochondria. ofPARL expression in of mitochondrial mass and energetics and elevated oxidative in PARL reduction siRNA-mediated protein in results alowering T2DM. with individuals andsubjects in themuscle ofelderly ed protein is correlated to mitochondrialcontent anddownregulat protease PARL. hasrhomboid shown that PARL laboratory Our of theorganelle. Inmammals, by the is fusion regulated in part and fission) for maintainingpivotalareprocesses function the tion func muscle of skeletal on PARL P.I.M.A-2: effects The (i.e.,skeletalsarcopenia) muscle fibers age.old in uncoupling andoxidative damage andeventually to loss of canProlonged lead ROS to production severe mitochondrial ROS production. tion leads to incrementaldamage andfurther ensue can ROS in produc which increased cycle destructive and proteins, lipids,mtDNA, aself-perpetuating andmembrane damage cause ROS can to As (ROS) species production. oxygen capacity, reduced mitochondrial content, and elevated reactive insulin loweroxidation muscle resistance, skeletal with ated diseases, and some cancers. Both aging Both cancers. diseases, andsome 2diabetes mellitus (T2DM), Alzheimer’s andParkinson’sas type component ofmany associated aging, with such pathologies Why mitochondria? is Mitochondrial an important dysfunction disease. metabolic for drug-mimetics in thearea ofpeptide has program a vigorous thelaboratory BioPharma L.L.C., Exergy P.I.M.A-2 andE3-L-R (see below). studies Incollaboration with the from obtained in skeletal muscle samples studies functional is II currently c Laboratory formed newly The ing, and(2) development targets. preclinical ofdrug 2diabetes insulinmellitus,wasting, andag resistance, type relationship andits to muscle anddysfunction al remodeling mechanism(s) themolecular and characterize ofmitochondri FOCUS SCIENTIFIC REPORT . Recent work shows that mitochondrial remodeling (fusion : The goals of this laboratory are: goals (1) ofthis The laboratory to understand John S. McIlhenny S. John Skeletal Physiology Muscle onducting cellular,onducting molecular, and

2 010–2011 and T2DM are associ are T2DM and ------a combinatorial space.a diseases,in or as single either agents metabolic for therapeutics andproteins peptides as potential for tion is to utility identify explora ofthepreclinical goal The skeletal muscle metabolism. agonists in withdevelop selective peptide hormone-mimetics Drug-Mimetics transcription. DNA CR-mediated and in relation to CR, metabolism, ofR-0 thefunction understand to better WeT2DM. are currently KO-model generatingarodent in aging and observed widely defects two metabolism, redox improvemore, can we have glucose and that demonstrated R-0 Further humans. in states insulin-resistant in downregulated is upregulated by CR and evidence that R-0 thefirst and provide exciting are data Preliminary regulation. gene and function, (name that is coded) regulates processing, DNA histone“R-0” II McIlhenny S. John Iand Skeletal Physiology Muscle Laboratories mechanism(s) as to theunderlying is uncertainty still The ofCR. cardiovascular disease, as well as overweight andobesity. There cancer, diseases including T2DM, ment ofage-associated and StudyE3-L-R insulin resistance. regulation, mitochondrial and the development function, of PARL,relationships weight between ofaging, body biomarkers of overexpression muscle-specific with the study PARL and to Tg oftheP.I.M.A is objective to phenotype study miceprimary (see ofmitochondria figure). theinternalchanged structure The oxidativedamage, and insulin increased signaling, impaired muscle oftransgenic (Tg) mice lowered mitochondrialcontent, Lene Hjelle, M.Ph. Hjelle, Lene Team:Research Ph.D.Anthony Civitarese, Faculty: (+) (+) Representative transmission electron microscopy of cross-sectional slices of PARL positive muscles at high (x20,000) magnification have discoverednewly a identified E3 ubiquitin called ligase (Panel A) (Panel PENNINGTON RESEARCH CENTER BIOMEDICAL and PARL and (-) negative ( . CR has been shown to. CR hassuppress thedevelop been . We have to program analog an active . Panel B Panel ) mitochondria in transgenic mice - - - CLINICAL RESEARCH 57 ------010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Meghan Greeley (Joint Program Coordinator),

Angela Charron, RN (Research Coordinator); TimAngela Charron, RN (Research Coordinator);

The program is focused on training at all levels, and and The program levels, focused all is at on training Robert Dubin, MD; Kamran Rasul, MD

foods. Training: students and house officersare trainedvariety in a of settings: public clinics, clinics, private didactics, discus group and small care and outside vital are reading components Patient of sions. the endocrine experience. opportunities training Research are The Program based of the Joint component in available. also the Division of EndocrinologyNew Orleans (i.e., and Metabolism of the Department of Medicine the LSUHSC School at of Medi pleased forCouncil offer two is Graduate to Accreditation cine) for Endocri accredited fellowships Medical (ACGME) Education Diabetes,Metabolism and # 1432112193). (Program nology, evaluated. In addition, the program has trials that are evaluating evaluating are that the program trials has evaluated. In addition, on mono currently and are diabetes had who have individuals therapy with a single agent, combination therapy with several The conducting program also is therapy. oral agents, or insulin presents diabetes how studies a in with the goal of evaluating Vietnamese). minority (e.g., the New population in Orleans area The interests of the program research basic Research: Basic been interdisciplinary dedicated approaches meta to to have bolic research, with particular interest in evaluating mecha of action nutrients which interventions nisms and nutritional by sensitivityenhance insulin in muscle and adipose In this tissue. faculty laboratory basic in involved regard, the program’s are investigation and mechanisms operable in both models animal subjects. and human being currently evaluated Interventions include fatty acids, the mineral trace and chromium, specific bioactives their as to effect in action.modulatingcellular insulin In addition, experiments being are conducted the determine to of dietary the oral in sensing involved fat animal in mechanisms models the important and investigate to relationship between feeding and reproductive behaviors, particularly investigating neuropeptides affect of energy-dense that consumption the Research Staff: Research Allerton, (Research MS Bench, Eli Associate); (Research BS Associate) Staff: Administrative Sehzad Kristina McMichael SooklallCoordinator), (Fellowship (Business Manager) Fellows: ------in New

Jeffrey Gimble, MD,

Robert Richards, MD; Gabriel

William T. Cefalu, M.D. William T. Our areas of expertise Our areas and include the diagnosis The Joint Program on Diabetes, Endocrinology, and and Endocrinology, Diabetes, on Program The Joint :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS tise in endocrinology and clinical research at Pennington endocrinology in tise Pennington at research and clinical Biomedical been has aligned with the medical care provision efforts training and education endocrinologyand fellowship Program. The a seamless Joint creating LSUHSC,at thus jointly who is MD, Cefalu, T. led William Program is Joint by the facultyappointed to and whose both institutions time at dividedallocation is between the two approxi at institutions 50% effortmately each. at The faculty Program the of Joint either or both institutions. to are appointed Metabolism is a single academicMetabolism a single program is two across distinct Program the Joint Specifically, campuses of the LSU System. effortrepresents a collaborative betweenPennington the Rouge Baton and the LSU in Biomedical Center Research (LSUHSC) School of Medicine Center Sciences Health Orleans. The purpose of the Joint Program is to enhance the Orleans. the The purpose enhance to Program is of the Joint research, education, and medical care in diabetes, endocri both so institutions, as metabolism and aligning by nology, and of resources, sharing facilitate collaboration, promote to of efforts. duplication avoid Therecognized research exper The program is heavily involved in many many in Research: TheClinical involved program heavily is type evaluating trials clinical and its compli 2 diabetes related ment, osteoporosis screening, and management and treatment treatment and management and screening, osteoporosis ment, disorders.of thyroid patients who evaluate that trials have regard, we In this cations. prediabet very considered are process (i.e., the disease early in and for whom lifestyleic) being is intervention and nutritional Clinical Care: Clinical The Program provides Joint a wide range of services including supervision, resident and both care, studentpatient teaching, research. and clinical basic and endocrine of diabetes treatment abnormalities. Our clinics and diabetes obesity andcomprehensive treat offer evaluation PhD; TimPhD; Donna PhD; Church, MD, Ryan, MD; Eric Ravussin, Frank PhD; MD; AlokGreenway, Gupta, MD; Catherine Gettys, Champagne, Tom PhD; BethPhD; Indu PhD; Kheterpal, Floyd, Don PhD; Ingram, Zhong PhD; Wang, MD Faculty (Adjunct) based at Pennington Biomedical: Chiefof Joint Program: Faculty based at LSUHSC-New Orleans: Uwaifo, MD; Karen Friday, MD; Primeaux, DeSilva, Stefany Uwaifo, MD; MD; Karen Taniya PhD; Friday, Lan Melendez, Yvonne PhD; Luu, Chi MD; Scharalda Jeanfreau, DNS, FNP-BC Joint Program on Diabetes, Endocrinology, and Metabolism Endocrinology, Diabetes, on Program Joint 58

CLINICAL RESEARCH Clinical TrialsPharmacology-Based Professor Frank Greenway, M.D. ingredient. This extract was given to volunteers, andtheir blood Thisingredient. extract flower known amountwas created of active root the with a theballoon from An extract andmenses. pregnancy for only vessels safe, since be should newblood healthy need adults vessel growth Inhibition of blood in rodents. to obesity reverse inhibitors have vessel growth that theability suchknown blood vessels in thelaboratory, ofnewblood is andit the growth We kimchi. that flowercalled demonstrated theballoon inhibits in theKoreanof radishandused that in diet afood is pickled type a flower rootballoon ofthe The is efficacy. supplements’ the demonstrating by studies tising claims supported be must andadver are regulated as supplements foods, herbal Dietary vessel disease (see figure). andblood and arisk heart for diabetes with associated closely that is most fat the abdomen, that treatment orlistat resulted inside offat in the adecrease thetreatment for aprescription ofobesity.without We showed approved adrug trial oforlistat, IV aphase We performed to by patients thewounds. inulcers diabetic delivering oxygen to show that study nitrite will helptoproof-of-concept heal foot could preventrelease, plana was not elevated. oxygen We next which were andthat safe methemoglobin, nitrite in theblood formulation. Werelease andaslow-release that found levels of ofnitrite, forms Itrial,In aphase we an testedtwo immediate approval (phase IV). to trials large (phaseIII), drug-approval to trials after ofdrugs tive, to (phaseII), ofthedrug trials dose determining theproper trials is determiningthedrug effec whether proof-of-concept in man (phase used been I),newhas adrug time first to early in which those is it pharmaceutical the trialsOur from range Current Projects herbal supplements, foods, and medical devices. andmedical foods, supplements, herbal in theareas ofpharmaceutical development, dietary obesity FOCUS SCIENTIFIC REPORT : The outpatient on clinicalThe focuses trials program Associate Professor Associate M.D. Gupta, Alok

2 010–2011

Boyd Professor Boyd Bray, A. M.D. George - - vate grants and contracts Research in this unit is by supported multiple public and pri and stimulate economic development in Louisiana. improve epidemic, health, public theobesity on positive impact will have novel anddevices.Wea that foods, efforts these hope supplements, herbal research into newpharmaceuticals, dietary cal’s Outpatient Clinic is through addressing this need unmet treatment. Pennington medical lence andhas good no Biomedi that is is problem aserious growing in preva Obesity ulcers. complications medical such asfoot diabetic andits of obesity outpatient thetreatment on clinicalThe focuses trials program treatmentas a obesity. for extract flower root balloon of the andto dose theeffect test thebest to identify are planned studies Further vessel growth. inhibitors ofblood takenwhen orally, unlike we have other foods with identified into thebloodstream andabsorbed was safe tory. extract The vessel formation in to thelabora was able inhibit newblood Aimee Yoches, Yu, L.P.N.;Aimee Ying M.S. B.S.; Windham, Aubrey B.S.; Thibodaux, Jolie Strate,Allison R.N.; Tance B.S.; Sonnier, Smith, B.S.; Marisa R.Ph.; Sides, Charles Reed; Lura J.D.; R.D., Rebello, Candida R.D.; L.D.N., Rajapho, M.P.H.; Rachal, Dawn Vimala B.S.; Phillips, Kimberly Perrault; Jennifer Marcell; Kimberly R.N.; L.P.N.; Mancuso, Susan Lockett, Monica Ph.D.; Liu, Ann B.S.; Lingle, Melissa R.N.; Prarie, La Stacie B.A.; L.P.N.; Jolivette, Pamela Johnson, Carolyn R.N.; Ihrig, Jana Frances Hutchinson; R.Ph.; Hazlett, Claire R.N.; Harrington, Lauren R.N.; Hamilton, Debbie Guy; Linda R.D.; L.D.N., Fahr, Gildersleeve, Bethany Janet B.S.; B.S.; Eldredge, Angela R.D.; L.D.N., Dragg, Crow, Amber Mavis L.P.N.; Currier, Leslie R.N.; B.S.; Cox, Lauren Burnett; Beth Mary R.D.; Boley, L.D.N., Karen B.S.; Bayham, Brooke R.N.; Arceneaux, Jennifer R.Ph.; Angelle, P.A.; Monce, Patricea Ronald (Clinic Director); R.D. Shipp, Mandy Team:Research M.D. Woltering, Eugene Ph.D.; White, Marney Ph.D.; Whisenhunt, Brooke M.D.; Ph.D., Raum, William M.D.; Paige, John M.D.; McClelland, John Ph.D.; Zhijun Liu, M.D.; LeBlanc, Karl M.D.; Hausmann, Mark M.D.; Guillot, Thomas Bellanger,M.D.; Drake Faculty: Adjunct PENNINGTON RESEARCH CENTER BIOMEDICAL Director for Clinical Research for Director Professor, Associate Executive M.D. Ryan, Donna . Donna Ryan, M.D. Ryan, Donna Bray, A. M.D.; George M.D.; Gupta, Alok Frank Greenway, M.D.; Faculty:

- - - - CLINICAL RESEARCH 59 - - - - - 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Faculty: Robert L. Newton, Ph.D. Jr., Research Team: Desti Shepard, Ph.D. existing from data Jackson the epidemiologi an Heart Study, cal study of cardiovascular disease risk in African-American in cal risk study disease of cardiovascular participants. of these A subset participants accelerometers wore providing objective of activity measures as were they while cal inactivity factors. risk with cardiovascular In the long run, we between be the relationship will assess able to these measures and actual occurrence. disease cardiovascular of be maintenance technology in utilizing assist also Our to is lab sessed for cardiovascular disease risk factors. risk sessed disease for cardiovascular allow will data This between the association assess to us activity physical and physi across ubiquitous becoming are phones Mobile change. havior technology being and in utilized increasingly is the country, tech utilizes developed that intervention an We care. health activitynology addresses as of physical the lack well as the in The children. P-Mobile be will intervention delivered nation’s parents, viato their mobile with phone, the goalincreasing of activity physical will the recommended We to levels. children’s between child a designated recruit the ages who families have old years or either obese. who overweight is The of 6 and 10 be will intervention conducted the course of three months. over The P-Mobile technology and related be will intervention adapted African-American to populations. laboratory supported is from the in this grants by Research Unit Research Nutrition Clinical the of Health, National Institutes and Coca-Cola the Center, Biomedical Research of Pennington Foundation. - - - The laboratory goal study of this to is the impact of :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS physical activityphysical and inactivity minor of ethnic on the health ity populations. ity populations. Our lab is currently focused currently Our is lab on studying different several aspects of inactivity the Sedentary through African- in Behavior the energy assessing of cost are We study. Americans (SeBA) typical sedentary reading, TV, behaviors, watching including and typing, body to The composition. relationship and their provide objective to goal is of SeBA on data the energy of cost activities for African-American adults because metabolic rates also are We studying the differamong populations. ethnic relationship between objectively measured inactivity and risk utilize will we study, factors this For disease. for cardiovascular creased physical activitycreased physical and increased inactivity been have levels be independent to shown factors risk of for the development and diabetes, disease, cardiovascular including diseases chronic been African-Americans has It also that shown obesity. spend less time in activity and more time in inactivity recom is than mended. Thus, African-American adults prime are targets for studying between the relationship activity/inactivity physical and chronic disease. find effective to goal is ultimate The lab’s activity physical increase strategiesbehavioral to decrease and sedentary minorities. ethnic in behavior African-Americansdisproportionately suffer from health various De diabetes. and hypertension, obesity, including conditions, Robert L. Newton, Ph.D. Jr., Assistant Professor Physical Activity Minority Ethnic and LaboratoryHealth Physical Preventive Medicine Laboratory

Faculty: Timothy Church, M.D., M.P.H., Ph.D., Neil Johannsen, Ph.D.

Timothy Church, M.D., M.P.H., Ph.D. Neil Johannsen, Ph.D. Professor, John S. McIlhenny Endowed Instructor Chair in Health Wisdom

FOCUS: The goal of this laboratory is to understand the role Research Team: of physical activity and weight loss in the prevention and Ronald Monce, P.A.-C.; Kimberly Kramer, M.P.H.; Ruben Rodarte, treatment of chronic diseases such as diabetes, disability, M.B.A., M.S.; Markee Baltazar, B.S.; Nathan Britt, N.P.; Benjamin Butitta, B.S.; Shannon Cocreham, B.S.; Sheletta Donatto, R.D., mental health, cancer, and heart disease C.D.E.; Melissa Lupo, B.S.; Melissa Harris, B.S.; Corbin Lemon, B.S.; Latrica Peters, B.S.; Meghan Duet, M.S.; Heidi Millet, M.P.A.; Chelsea Ashford, B.S.; Johanna Veal, B.S.; Ashunti Pearson, M.S.; In November 2010, the Preventive Medicine Laboratory Gina Billiot, B.A.; Damon Swift, Ph.D.; Ami Parks, M.P.A. published the results of a large National Institutes of Health (NIH)-funded clinical trial titled HART-D in the Journal of the American Medical Association. The goal of the Health Benefits light on this issue. The SPRINT study is examining the risks of Aerobic and Resistance Training in individuals with type 2 and benefits of maintaining systolic blood pressure below 120 Diabetes study was to compare the effects of nine months of mmHg compared to below 140 mmHg in older individuals with resistance training alone, resistance training in combination hypertension. While the population-based data suggest that with aerobic training, and aerobic training alone on hemoglo- lower blood pressure is better, there are remarkably few clinical

bin A1c in sedentary women and men with type 2 diabetes. We trials that have directly addressed this very important issue. found that while resistance and aerobic training alone each had The findings from both of these studies will provide valuable

benefit to hemoglobin 1cA , the combination of resistance and outcomes data that will shape future clinical guidelines related CLINICAL RESEARCH aerobic training had the greatest benefit. This was also seen for to the use of aspirin and setting optimal blood pressure ranges. changes in fitness and fat loss. It is important to note that on In addition to the above, we have ongoing pilot studies examin- average each of the exercise prescriptions took approximately ing the benefits of exercise in individuals with post-traumatic 140 minutes per week. stress syndrome and in reducing depressive symptoms in indi- We are currently in year 3 of the NIH-funded Lifestyle Interven- viduals with dementia. tion and Independence for Elders (LIFE) study, which is examin- Research in this laboratory is supported by grants from the NIH, the ing the role of physical activity in preventing disability in 70- to Coypu Foundation, the Edward G. Schlieder Educational Founda- 89-year-old individuals with existing function limitations. We tion, Coca-Cola, and the Pennington Medical Foundation. are one of eight U.S. study sites and recently completed recruitment six months ahead of schedule as we enrolled our 208th participant. We have partnered with the East Baton Rouge Parish Recreation and Park Commission (BREC) and the Baton Rouge YMCA to create more convenient intervention sites for the participants, and this appears to be working, as demonstrated by our very high compliance and retention rates.

We just started two new NIH-funded multisite trials titled ASPREE and SPRINT. Both studies are focused on preventing cardiovascular disease and dementia. ASPREE is examining the role of aspirin in preventing cardiovascular disease and dementia in individuals without a history of heart disease or stroke. While it is widely accepted that aspirin is of benefit for individuals with a Results from HART-D demonstrating the benefit of nine months of resistance training history of heart disease, the benefit of daily aspirin in individu- alone, aerobic training alone, and the combination of aerobic and resistance training on

als without a history of heart disease remains a topic of great hemoglobin A1c in individuals with type 2 diabetes. debate. The ASPREE study will help shine some much needed

60 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER CLINICAL RESEARCH 61 - - - - - 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC weight and/or insulin action insulin restore normal reproductive and/or weight function with women polycystic in ovary syndrome. an is This important study hopefully will of the development that lead to fosters programmanagement a specificthat reproduc weight tive health for affected women health tive theby syndrome. Hospital Woman’s with strong collaborations The laboratory has Karen Elkind-Hirsch, with Dr. Together Rouge,(Baton Louisiana). Woman’s at Center Research DirectorPh.D., of the Woman’s study conducting Redman in is eight-month an Hospital, Dr. whowomen gestational had a pregnancy in diabetes the within during preg diabetes who develop past Women four months. impaired susceptibility of developing glucose a high nancy have and typetolerance 2 diabetes. The objective of the study implement a structured,called to PRIDE is behavioral-based program focuses on management diet and physical that weight activity postpartum months between and on reten 4 and 12 of type and prevention gain weight 2 diabetes. tion of excess mothers after face Recognizing many the challenges birth, we the efficacy program management evaluating are weight of a traditional a to comparison in telephone the over delivered program. clinic of a pres the receipt Redman is for Dr. development exciting An program management implement a weight to NIH grant tigious and obesefor overweight women. In collaboration pregnant Martin lead the will Redman and Dr. Hospital, Dr. with Woman’s study called Expecting of Management Personalized Success: The study 300 during Pregnancy. follow will Body moth Weight ers and offspringthroughout pregnancyafter andyear for one birth. NIH, from the laboratory funded is in grant(s) this by Research C.B. andthe Botanicals Foundation, the and Pennington Irene and Feasibility Pilot Program. Center Research Faculty: Leanne M. Redman, M.S.Ph.D. Research), (Clinical Research Team: Karen R.D., Boley, L.D.N.; Marisa Smith, B.S.; Elizabeth Frost; Abby Duhé; Sarah Tavernit; Kimberly Phillips - The laboratory goal conduct of this to is clinical :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS and translational research studies better research to understandand translational the impact diet and including of the obesigenic environment, span. the life across health activity,physical on women’s In a newly awarded grant from the National Institutes of Health of Health from Institutes In a newly grant awarded National the Redman conduct will a randomized trial controlled Dr. (NIH), with women polycysticin ovary syndrome. The objective of the study characterize body in called changes to how is PULSE Dr. Redman has a strong interest in women’s health. Her current Her health. current a strong Redman women’s in has interest Dr. polycystic in is research ovary syndrome, gestational diabetes, and pregnancy and local funding received national and has to support her research group. In collaboration with Dr. Diana Thomas, Ph.D., at Montclair State State Montclair at Thomas, Diana Ph.D., with Dr. In collaboration Heymsfield, University Steven including colleagues and other Biomedical, Dr. Martin, of Pennington M.D., and Corby Ph.D., Redman participated of and validation the development in predict and accurately models that under- how mathematical overfeeding individual. the body of a given alter will weight model developed was a mathematical and Most recently, during pregnancy gain weight characterize to healthy validated sustain required to define of energytheamount intake and to new model This gain. designed was gestational weight healthy gestational weight national achieve women pregnant help to gain recommendations. ing in clinical physiology under Dr. Eric Ravussin, and she is an an and she is Ravussin, Eric physiology under clinical ing in Dr. expert physiology and metabolism human in studies and in that activity dietuse and physical (body energy balance regulate to and energy metabolism. weight) Dr. Redman recently founded the Reproductive Endocrinology Reproductive the Endocrinology founded recently Redman Dr. of train years several has She Laboratory. Health and Women’s Leanne M. Redman, Ph.D. Assistant Professor Reproductive Endocrinology and Women’s Health Laboratory Health Women’s and Endocrinology Reproductive 62

CLINICAL RESEARCH Sleep HealthCenter Professor Ph.D. M.D., Pan, Weihong structive sleep apnea will greatly reduce his health risks in vascular and metabolic diseases. diseases. metabolic and vascular in risks health reduce his greatly will apnea sleep structive severe of ob panel). treatment (lower Successful is improved architecture sleep treatment, BiPAP Withsuccessful apnea. sleep isby This caused sleep. severe obstructive movement eye rapid and sleep Stage restorative slow-wave is is in 1(light)sleep;no there sleep the of Most fatigue. daytime and apnea, snoring, of 45 of symptoms and index mass abody with man is a subject human The quality. on sleep obesity of Fig.1. effects the of extreme The vivo electro ex tings ofclinical studies, research, sleep mouse set in ofscientificthe questions in nature, avariety we tackle neuroinflammation, and neurodegeneration.Being translational relatedexistence to recently to obesity, launch several projects PenningtonHealth CenterThe Sleep into Biomedical came to maximize human potential by improving neuroplasticity. neuroplasticity. byimproving potential human to maximize ing ofthecerebralin an mechanisms effort disorders ofsleep advanceto our understand expected findings aretions. The func cardiovascular andmetabolic andimmune systems, neurochemistry, autonomic control system nervous of the neurobehavior, manipulationsdetermine how sleep affect in different diseasessleep and their animal and models to FOCUS SCIENTIFIC REPORT : The focus of the Sleep Health Center oftheSleep focus : The is to phenotype

2 010–2011 - - - - - tutes Health of andother extramural funding is available. Pennington Biomedical Research Center, until National Insti Research in this unit is by supported asmall startup from fund pathological conditions. research tocircadian rhythm ahigh involvement level its with in and assays, to sleep andhistological we bring ological, hope neurobehavioral quantification, and biochemical, electrophysi combination recordings, with telemetry metabolicanalyses, zero. ground labfrom In hasthe sleep progress madesteady anddespite challenges, program, which therewasprior no this was initiated conditions.tal As program at theCenter for controlled experimen rigidly with procedures restriction sleep analysis, andchronic spectrum recording, electrocardiogram lab contains sleep mouse The 10 ofbaseline andis units capable (AASM) level II monitors. ing by devices that Academy Medicine are American of Sleep monitor portable lab, sleep as wellbed as full-spectrum with in atwo- andovernightgist, is performed polysomnography neurolo sleep evaluationsleep by is aboard-certified provided exercise andsleep. Clinical sleep, between andinteractions trials,in obesity interactions endocrine disorders and between human lab collaboratesThe sleep investigators with engaged disease and major depression. andtheir clinicaltion duringsleep significance in Parkinson’s than normal)situations; (4) mechanisms ofocculomotor regula oftheregulation (rather ofpathophysiological understanding pathways ouring rather than to neuronal projection expand regulation by focusingglial on homeostasis cellof sleep signal Parkinson’s disease; (3) revisiting and challenging the theories such as Alzheimer’s disorders diseaseneurodegenerative and multiple sclerosis,for in mice adult-onset with obesity, andin in autoimmune experimental encephalomyelitis (EAE), a model (2) in neuroinflammation, ofsleep role such as thefunctional and how thesignals are integrated system; in an organ whole or manner region-specific andbrain in acellarchitecture type- include: (1) studies Ongoing modulates sleep how acytokine profiling. in thefuture, metabolic of thebrain,and, hopefully magnetic resonance imaging (fMRI) functional analyses, ability combine polysomnographic recording rate with heart vari We analyses. recording,physiological andcircadian also rhythm (Adjunct) Wang, Ph.D. Ph.D.Wei-Hsung (Adjunct); II, Matthews Kenneth (Adjunct); M.D. RPSGT; Foundas, L. Salmon, Anne Maniphanh Team:Research Professor Ph.D., M.D., Pan, Weihong Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL -

------CLINICAL RESEARCH 63 ------010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Faculty: Geiselman, J. Paula Ph.D. Research Team: Megan M.A.; Apperson McVay, Justin Ericson, M.A.; LaVigne; MalloryTara Taylor McGuffey; Miller. and ronutrient content of the craved foods females. of the craved in In addition, content ronutrient reliability scalestest-retest of the three for craving each was for each). strong < .001 (p Bio being is used studiesThe numerous Pennington in FPQ at Insti beingmedical. is used the National at In addition, the FPQ and universities institutions other and at (NIH) of Health tutes Canada, States, country this in the world and around (United Kingdom,United South Africa, and Australia). funded U.S. the was Department by The development FPQ of the of laboratory sup The above-reported is in this Agriculture. research Institute. Science Wrigley ported from the grants by hydrate and fat content (p < .001) were the second predictor were < .001) (p and fat content hydrate variable the entered regression into equation. scales craving for sweet, validated these results have Hence, high-carbohydrate,high-fat and foods with respect the mac to night fast) across the two fast) across night study visits. the that regression multiple analyses revealed Stepwise, for specific females’ cravings and foodssugar fat in high both provided the best predictorcontent for sweet for cravings their the best as well as predictor for cravings their foods < 001), (p No predictor other vari for high-carbohydrate foods ( p < .001). for these regression Cravings equations. into entered ables were also provided specificcontent and foodssugar in fat high both for high-fatthe best cravings foods predictor for the women’s for foods both in complex cravings and high carbo < .001), (p conducted four weeks later to determine test-retest reliability. conducted reliability. four weeks test-retest determine to later for the effects controlled we error reduce variance, of female To sex a 10-hour hormones over status and nutritional (following - - - - The objective laboratory study of this to is the role :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS of fat and other macronutrient intakes and fat preferences in and fat preferences in intakes macronutrient of fatand other food of and bodythe control intake weight. cific foods in which fat content varies significantly andsignificantly varies system cific foodscontent in which fat Women rated their cravings for sweet, cravings their rated high-carbohydrate, and Women high-fat the modified foods. Using Food Preference Geiselman subjects for spe cravings their rated also Questionnaire (FPQ), cally. The primary of the present study validate cally. aims to were food scalescraving with respect the specific to macronutrient reliability foods test-retest assess in of craved and to content validity validityfemales. Concurrent also discriminant and were assessed. content. and protein complex carbohydrates, with sugar, atically were they that the cravings assessments, rated women In all experiencing the present moment. A second was at test visit Food cravings are the most Food are frequently cravings for reason cited non Food cravings are mostFood are often cravings assessed asking by subjects to for sweets, cravings their and high-fat rate carbohydrates, these the types are that shown foods, has research as of foods not these scales have most are typicallythat However, craved. obtained data be interpret able with these to been To validated. the types scales, validate craving critical it to is of foods craved foods using in with respect content, the macronutrient to and varies significantly systemati content macronutrient which compliance with dietary regimens, and research has shown that especially for more cravings, significantly sweets, have females than do males. Paula Geiselman, J. Ph.D. Associate Professor Women’s Health, Eating Behavior, and Smoking Cessation Laboratory Smoking and Cessation Behavior, Eating Health, Women’s POPULATION SCIENCE

64 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER Background

The establishment of Population Science as a research focus public health strategies that can be employed to tackle the was a priority in Pennington Biomedical’s five-year strategic growing problem of childhood obesity. plan, Vision 2010. This goal was realized in August 2007 when The second major public outreach effort initiated by the Popu- the position of Associate Executive Director for Population lation Science faculty is Louisiana’s Report Card on Physical Science was created. In the past four years, we have added five Activity & Health for Children new Population Science laboratories at the Center, in addition and Youth. The primary goal to two existing ones, to complement our research strengths in of the Report Card is to assess Basic and Clinical Science. In addition, we have expanded our the level of physical activity program evaluation efforts, and we currently are the external and sedentary behaviors in POPULATION SCIENCE evaluator for the Louisiana Department of Health and Hos- Louisiana’s children and youth, pitals Tobacco Control Program and the Blue Cross and Blue the facilitators and barriers of Shield of Louisiana Foundation’s Challenge for a Healthier physical activity behavior, and Louisiana grant program. related health outcomes. The Current Status Report Card, which is currently in its fifth year, is an advo- Peter T. Katzmarzyk, Population scientists at Pennington Biomedical are involved cacy tool designed to increase Ph.D., FACSM in research, evaluation, education, and scientific advocacy awareness of the health con- Professor, LPFA Chair activities. Our population scientists are studying health issues cerns of Louisiana’s children. in Nutrition, Associate Executive Director for at the level of the community and population rather than in The main target audience of the Population Science a laboratory, with the overarching mission of improving the Report Card is adult decision health of the population. Our research efforts in Population makers, and through this effort, Science initially focused on nutritional epidemiology and we hope to provide a level of health care delivery. We have recently expanded our focus by accountability on behalf of the adding laboratories in contextual risk factors, walking behav- children and youth of Louisiana. ior, physical activity and obesity epidemiology, comparative effectiveness research, and chronic disease epidemiology. All The Future of the Population Science laboratories contribute to the mis- According to the World Health Organization, about 80% of all sion of the Center and expand upon the research strengths in deaths worldwide are the result of non-communicable dis- Basic and Clinical Science. eases (NCDs), or chronic diseases. On September 19-20, 2011, In addition to our research and evaluation efforts, the Popula- the United Nations held an historic high-level meeting in New tion Science unit has developed two initiatives in education York City to address the growing global burden of NCDs and and scientific advocacy. First, for the past four years, Pen- to challenge the world’s nations to focus on the prevention of nington Biomedical has organized an annual public health NCDs. The situation is even more acute in North America. An conference on the topic of childhood obesity, which is quickly aging population, coupled with an increasing prevalence of becoming the greatest public health challenge facing Ameri- obesity, does not portend well for the health of the popula- ca. During the past three decades, the number of overweight tion. In addition to individual-level behavior changes, large- and obese children has skyrocketed, resulting in a growing scale population shifts in normative behavior are required to number of children facing health consequences that were see real reductions in NCDs and improvements in population traditionally experienced only by adults. This public health health. The population scientists at Pennington Biomedical conference brings together approximately 500 local, national, Research Center are well positioned to make major contribu- and international experts on the topic of childhood obesity, tions to seeing these much-needed health improvements with a focus on prevention. The major goal is to develop through to fruition.

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 65 Chronic Disease Epidemiology Laboratory

Faculty: Gang Hu, M.D., M.P.H., Ph.D., F.A.H.A.

Research Team: Yurong Zhang, M.D., Ph.D.; Yujie Wang, M.Sc.; Wei Li, B.Sc.; Wenting Xie, M.Sc.

Gang Hu, M.D., M.P.H., Ph.D. Assistant Professor

TGDPP: This project is an ongoing three-year lifestyle interven- FOCUS: The goal of this laboratory is to assess the role of tion program among 1,180 Chinese women with gestational dia- lifestyle and other risk factors and their interactions on the betes mellitus (GDM) at least one year after delivery. The specif- risk of chronic diseases including coronary heart disease, ic aims of the TGDPP are: (1) to test the hypothesis that lifestyle stroke, heart failure, diabetes, cancer, and Parkinson’s disease. intervention can reduce incident type 2 diabetes in women with prior GDM; (2) to evaluate the interactions between lifestyle The Chronic Disease Epidemiology Laboratory is collaborating intervention and variations in established genes for glucose, with several large-scale epidemiological studies and clinical insulin resistance, lipids, obesity, and type 2 diabetes in relation trials, including the Louisiana State University Hospital-based to metabolic traits for diabetes in women with prior GDM; and Longitudinal Study (LSUHLS), the FINRISK Study, Tianjin Gesta- (3) to assess the effects of a lifestyle intervention program in tional Diabetes Prevention Project (TGDPP), and Tianjin Children mothers with GDM on their offspring’s health status, including Obesity Study. childhood obesity and metabolic abnormalities.

LSUHLS: Louisiana State University Health Care Services Division Tianjin Children Obesity Study: This ��retrospective longitu- (LSUHCSD) operates seven public hospitals and affiliated clinics dinal study includes 15,928 children aged 3 to 6 years in Tianjin, in different areas in Louisiana. Since 1990, the LSUHSC facilities China. have served about 1.6 million unique patients, representing ap- POPULATION SCIENCE proximately 35% of the Louisiana population. The LSUHCSD Dis- Research in this laboratory is supported by the Louisiana Depart- ease Management Evaluation Database contains administrative ment of Health and Hospitals, the American Heart Association, data, anthropometric and blood pressure measurement data, Merck, EFSD/Chinese Diabetes Society Programme for Collab- laboratory data, treatment data, and clinical diagnosis data. All orative Research between China and Europe, and International these data have been available in electronic form since 1990 for Diabetes Federation (IDF) Bringing Research in Diabetes to Global both inpatients and outpatients. Most patients in LSUHLS have Environments and Systems (BRIDGES). now been followed for more than 10 years.

The FINRISK Study: The FINRISK Study is one of the largest prospective epidemiological studies of chronic disease risk factors in the world. The total sample consists of 62,013 individuals African from Finland (30,031 men and 31,982 women) aged 25 to 74 Total White years. The follow-up is virtually complete through the nation- wide register linkages including the Causes of Death Registry, the National Hospital Discharge Registry, the National Social Insurance Institution’s drug register, and the National Cancer Registry. Follow-up databases have been recently updated, and outcome data as of the end of 2008 are available. Approxi- mately 13,000 deaths, 6,000 coronary heart disease cases, 4,000 stroke cases, 3,500 heart failure cases, 800 Parkinson’s disease cases, and 6,500 cancer cases have been ascertained during the mean 20 years of follow-up. Prevalence of asymptomatic diabetes and total diabetes in LSU hospitals

66 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER POPULATION SCIENCE 67 - - - - 010–2011 2

Conceptual framework that that framework Conceptual how contextual risk illustrates factors shape individual risk give factors, ultimately which rise to disease. from DanielAdapted et al. and 2008) & Place, (Health Med., Sci. & McAtee (Soc. Glass 2006).

SCIENTIFIC REPORT SCIENTIFIC (CVD) Disease Cardiovascular

Faculty: Ph.D. Broyles, Stephanie Research Team: Drazba,Katy M.P.H. T. risk. on specific shed will Results light research from path this screening, impetus for disease an targetedproved environmental efforts.and policy for future and a basis preventive change, study participating also of Our is international lab a large in obesity activity physical and Through children. 10-year-old in of a child’s be will look able we the contributions to at study, this neighborhood,home, school and diet and to environments activityphysical behaviors the world. across Uni State conducted the Louisiana In research at with colleagues ways linking of the neighborhood development ways to environment CVD during a critical risk time period, for im providing tools thus (LSUHSC) Schoolversity Health of Public Center Sciences Health studying are Newin Orleans, we neighborhood the role that parks capital” fostering in “social among play park have users. We parks that shown with social higher capital used more are by people and support activity amounts higher of physical than parks hope extend social capital. with to lower We research this programs parks developing within by surrounding their and neighborhoods social neigh capital increase cultivate to and to borhood activity park physical and use the parks. within laboratory supported is Heart in this American the by Research and Pennington of Health, the National the Institutes Association, Foundation. Research Biomedical Genes Obesity - pressure) Adiposity inflammation) Fat deposition Fat fat Abdominal Moderators (insulin resistance, & HPA Dysregulation& HPA (catecholamines, blood (catecholamines, Energy ExpenditureEnergy & Neuroendocrine loading Neuroendocrine Resting energy expenditure Resting energy Biological & Mediators Maladaptive regulatoryMaladaptive shifts dyslipidemia, oxidative stress, oxidative dyslipidemia, Autonomic Nervous System Autonomic Smoking Alcohol use Alcohol Energy input Energy & Lifestyle (physical activity)(physical Mastery & control Disadvantage (feeding behavior) (feeding Accessible capitals Accessible expenditure Energy Chronic Stress, & Stress, Chronic Health Behaviors Conscious & Non- Conscious Pathways, conscious Perception of environment Perception : The laboratory goal understand of this to is the how

of place, (local attributes Contextual Risk Factors Contextual social & physical environment) social & physical environment food Local of fruits/vegs, availability (e.g., of fast food) presence hazards Psychosocial social crime, (e.g., disorganization) Area deprivation Area crowding) poverty, (e.g., Built environments walkability, connectivity, (e.g., parks or other to access activityphysical facilities)

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS different contexts different live—e.g.,we in which neighborhoods, schools, work, parks, social networks—shape risks disease our outcomes. health and fiableaspects and policytheof social, physical, environments factors risk with individual health linked are that or behaviors. factors on focusing risk are we behaviors and the in Currently, and obesity. (CVD) disease of cardiovascular broad areas One project studying through the biological involves pathways neighborhood development to a child’s which contributes of early of CVD, obesity markers including and diabetes. The frameworkfor research this shown is in the figure. accompanying studying currently are the neighborhoodWe of environments 400 who children took part Pennington completed a recently in project This linking neighborhoodBiomedical study. involves characteristics food like retail outlets, opportunities for physical activity, and neighborhood safety data behavioral and disorder to and cutting-edge early CVD indicate biological that measures In recent years, a new epidemiological years, In recent paradigm has research factors risk traditional emerged that smoking, recognizes that like poor inactivity, and obesity diet, physical shaped themselves are The live. we which in environments the socialby and physical Laboratorygoal of the Contextual Factors Risk identify to is modi Stephanie Broyles, Ph.D. Broyles, Stephanie Assistant Professor Contextual Risk Factors Laboratory Risk Factors Contextual 68

POPULATION SCIENCE Health Behaviors andChronic Laboratory Disease Associate Professor Associate David Ph.D. Harsha, children, adolescents, and young adults. Pennington andyoungchildren, adults. adolescents, Biomedical relatingeconomic factors to the development in of obesity andcomposition, social,psychological, physiological, genetic, body examine of the interactions thatextensively will effort TroopThe Improvement Study is Recruit amulticenter (TRIM) adulthood. into and early childhood throughout development particular, research into ofthis has funded condition’s theroots in base own recruitment andits in general society impact Army, U.S. mightThe to which obesity cognizant ofthedegree musculoskeletal disorders. cardiovascular disease, stroke, diabetes, many and cancers, include conditions associated overweight with andobesity numerous anddevelopment.medical The in early growth ingly is increasing in childhood increas Onset andoccurring adults. countries, including theUnited States, in rates 60% now exceed increasingly over for globally been ageneration,andin many ing populations Rates around theworld. ofoverweight have conditions affect medical pernicious is ofthemost one Obesity and chronic disease risk in children andadults. traits, biomedical behaviors, therelationshipson between FOCUS SCIENTIFIC REPORT : The goal of this laboratory is to ofthis develop goal informationThe laboratory

2 010–2011 - - Department of Defense.Department Research by grants this in the isfrom U.S. supported laboratory thecurrent health ofoverweight. public reducing burden informing interventions andpreventive at aimed programs ment ofoverweight ever created invaluable andwill be in ofthedevelop picture extensive result themost The will be gathered. be also will characteristics community and school, weight. Data neighborhood, on development, andbody relatedand availabilityseasonal/cyclical to growth, factors issues security including commonly less examined such as food environments andeconomic in thesocial whichon they reside, childrenindividual andyouths, willinformation also but yield above. will characterize notoutlined only Information collected annually up to for fouryears andwill submit data in theareas followed1,700 will be participants These youths 3states. from theintensivePhase will II begin examination ofapproximately in 2012.begin to study thefull for protocols thestage andset data collection gatheredin 2011 are being children andadolescents to finalize tionships. Currently in Phase I(pilot), of smaller data on samples relais these offourclinical one sites gathering data to clarify Ph.D. Harsha, David Faculty: PENNINGTON RESEARCH CENTER BIOMEDICAL - - POPULATION SCIENCE 69 - - - 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Faculty: Ronald Horswell, Ph.D. Research Team: Yi, Ph.D.; Besse, Jay B.S.;Yong Xiaobing M.S. Fang, LSUHCSD’s clinical data warehouse (called DMED) to analyti to DMED) warehouse (called data clinical LSUHCSD’s management disease cally support division’s the and evaluate programs,and population programs health including for adult diabetes, chronic kidney asthma, HIV, disease, heart failure, hypertension, management, cancer weight control, tobacco The HQI Laboratory development. home medical and screening, platform,” electronic an developed has a “drilling interface that clinic, rapid of program summarization the site, allows results at supports and that level and physician case-mix and adjustment The HQI analysis. subgroup Laboratory L-DMED, created has also authorized by accessible warehouse, data clinical a limited been has extensively used epidemiological researchers. L-DMED Epidemiology Disease the Chronic Gangby Laboratory Hu) (Dr. Biomedical. Pennington at opportunityIn addition, a largely latent very using in lies de more focused generate tailed to informatics information health The HQI improvement. outcomes hypotheses regarding health Laboratory projects develop approach to pursuing this now is a cost-effective creating algorithm for (1) areas: the following in screening for diabetes and prediabetes conjunction (in with the incorporating (2) Laboratory), Epidemiology Disease Chronic defining retinal diabetic imaging into exam eye protocols, (3) devel and (4) use, targeted hypotheses for reducing tobacco of medical perceptions patient assess oping instrument to an home experiences. laboratory supported is in this an ongoing by Research contract study was Heart The Tele-monitoring Failure LSUHCSD. with Legislature. Louisiana from the LSU to funded a grant by - - Effect of home tele-monitoring program on utilization outcomes among heart failure patients. failure heart among outcomes utilization on program tele-monitoring home of Effect - : The laboratory meth research goal use of this to is

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS ods to determine how to improve the cost-effectiveness improve to of how determine ods to care delivery. health A recent example, with final analysis still in progress, was a in progress, still was analysis example, with final A recent trial” the effectiveness assess to “pragmatic tele- of home formonitoring heart reducing patients in failure adverse events Inpa (emergency department admissions.) visits and inpatient Improving cost-effectivenessImproving delivery of care health requires The Healthcare care tradeoff. cost/quality the health improving LaboratoryQuality (HQI) Improvement goal using pursues this redesigning care delivery (2) algorithms, methods: (1) several existing into technologies new incorporate to how evaluating quality improvement designing (3) deliverycare algorithms, informatics coupled using with programs and projects, and (4) hypotheses. test methods defineand research improvement to done conjunction largely in is work withThis the LSU Health Care Services and its and clinics at hospitals Division (LSUHCSD) population centers. Louisiana major seven of the HQI Laboratory emphasis A major the is cost- improve informatics to of health use effectiveness. The HQI Laboratory usesthe tient stays by heart failure patients are a major cost component heart stays by tient component cost a major patients are failure represents a admission such and each Americanin care, health The HQI high-risk event. Laboratory health avoidable potentially (study Lee Arcement studydesignedthis conjunction in with Dr. LSUHCSD an cardiologist lead for the division’s and clinical P.I.), heart done program. at management The disease failure study, four LSUHCSD randomized heart sites, either patients to failure Home tele-monitoring care. usual or tele-monitoring home and blood weight as subjects (such data monitoring daily had forwarded automatically a nurse to pressure) Results suggest review. for daily care coordinator reduce may tele-monitoring home such that of heart admissions patients by failure inpatient ac be may Theimprovement 60% figure.) (see a modest by companied the number in increase of primary care visits per person. Ronald Horswell, Ph.D. Horswell, Ronald Associate Professor Healthcare Quality Improvement Laboratory Quality Healthcare Improvement 70

POPULATION SCIENCE Nutritional EpidemiologyLaboratory Professor Ph.D. Champagne, M. Catherine ton Biomedical involve dietary counseling efforts. counseling involve efforts. ton Biomedical dietary atPenning projects Several Counseling Activities: Dietary to education promotegroups andnutrition healthier diets. are states involvedthree which focus has used in this project, grains, anddairy, whole within anetables, The adult population. veg fruits, on specifically Americans, for Guidelines the Dietary to strategies on improve in Arkansas leadership adherence to with our from on team working have focused been efforts Other sis vegetables, dairy, on fruits, and grains. whole intakes improved thechildren among due to an empha dietary that Itwas observed lessons. education nutrition and included 6through 13 aged students for lunch andsnacks provided years 2007tion ofsummer from camps through 2010. camps The comple andupon to prior thestart children Arkansas in rural intake informationsissippi. on We dietary have collecting been Louisiana, ofArkansas, region Lower andMis vantaged Delta at improvingin theimpoverished nutrition anddisad directed and out, evaluate interventions design,carry to research effort collaborative, ofan ongoing multiyear part has been Biomedical Pennington Delta: the in Improve Health to Strategies (OPRU) Unit – Research Prevention Obesity Delta The • • • tion andcounseling that improve andhealth. diet is to educa ofthisnutrition Another provide goal laboratory relationship between diet andhealth diet in humanrelationship between FOCUS order to adhere to the dietary targets. targets. toorder to adhere thedietary meal plans exchange or options in asked to follow structured were in protein subjects andfat; treatmentsdiet varying offour LOST POUNDS The trial testedtheweight loss effects individuals. diabetic of tion changes in apopula lifestyle on trial focuses AHEAD Look The will continue changes; this until project 2015.lifestyle DPPfollows have individuals who from successfully made Prevention Diabetes OutcomesThe Study (DPPOS) Project SCIENTIFIC REPORT : Nutritional includes epidemiology

2 010–2011

all studies oftheall studies

populations. populations. ------and the U.S. Army. U.S. the and Health, of Institutes National the Agriculture, of Department U.S. the from by grants supported is laboratory this in Research 2010). ber (March 2010), intervention initial 12 after and (Septem months toprior intervention (September 2009), 6months following baseline points: time three during intakes soldiers’ to capture photography by using food efforts these supported Lab ology remainder six months. after Stafffrom the Nutritional Epidemi the and initially intervention receiving half with facilities, dining Enhance 10 included Healthy study in Soldiers.” Nutrition The Dining Facilities in Military to Practices Serving of Modifying dining“Testing facilitiestitled in its theEfficacy ated astudy initi 2009. NC, Bragg, In2009,August October andearly Fort duringlate phases Candidates andCadre,” in two conducted andPhysiological Strain Forces in Special Expenditure “Energy was thesiteCamp Mackall,titled Forces NC, ofaSpecial study Research Institute ofEnvironmental (USARIEM). Medicine in Army collaboration theU.S. with haveies supported been Soldier Nutritional Epidemiology: Epidemiology: Nutritional Soldier M.P.H., R.D., LDN; Dawn Turner, Dawn LDN; M.P.H., R.D., B.S. Levy, Erma LDN; R.D., Cash, Katherine CDE; LDN, R.D., Cerniauskas, Barbara CDE; LDN, R.D., Begnaud, Michelle B.S.; Afton, Marlene Ph.D.; Allen, Raymond H. Team:Research FADA LDN, R.D., Ph.D., Champagne, M. Catherine Faculty: • ing nutrition informationing nutrition and behavior change messages. utiliz sessions individual and group both by conducting roles key played dietitians/interventionists research Laboratory the American Dietetic Association 2011 in theDecember published volume ofthe trial were changes resulting theWLM from Dietary extension. unfunded two-year a included project the Internetor efforts; contact personal ofeither period phase,second a30-month through a sustained best change sessions be can lifestyle determine how weight loss achieved in1ofintensive phase WeightThe Loss Maintenance to trial was designed (WLM) PENNINGTON RESEARCH CENTER BIOMEDICAL . Nutritional Epidemiology Since 1996, nine stud Journal of - - - - - POPULATION SCIENCE 71 70 70 White Black White Black 60 60 Women Men 010–2011 2

50 50 40 40 Body Mass Index Body Adiposity Index Body index mass 30 30 Body adiposity index SCIENTIFIC REPORT SCIENTIFIC 20 20 Faculty: Katzmarzyk, T. Peter Ph.D., FACSM Research Team: Tiago Barreira, Deirdre Ph.D.; Harrington, Ph.D.; Amanda E. Staiano, Ph.D.; Emily Mire, M.S. 10 10

0 0

10 10 70 70 30 30 50 50 20 20 60 60 40 40

Body Fat, % Fat, Body Body Fat, % Fat, Body Men and women differ in their level of bodyfatness for a given body mass index (BMI) or body adiposity as shown this by figure indexfromscientific a (BAI), paper thatused data from the PCLS and published was in the Journal of the American Medical Association This paper challenged the assertion a superior is that BAI 2011;306(8):828-830). (JAMA measure of body fat compared BMI. to ------The goal of this laboratory is to investigate the the The laboratory goal investigate of this to is :

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS effects fitness, activity, obesity and morbidity on physical of and mortality quantify and to impact their on population health. Research in this laboratory is supported by the National Institutes laboratory supported is in this National the Institutes by Research U.S. the of Department Health, Canadian the of Agriculture, Heart the Foundation Research, and for Health Stroke Institutes Foundation. Biomedical Research of Canada, and Pennington the sponse relationship betweensponse activity relationship physical how and health, activityphysical interacts with obesity factors risk and other in predicting the effect outcomes, health of sedentary on health mod versus television sittingbehaviors as such watching and the Bogalusa Activity Physical dian (PALS), Study Longitudinal Longitudinal Center the Pennington InSight, Heart Study, and Nutrition Health Examina and the National (PCLS), Study The PCLS represents a cohorttion Survey. who of volunteers Biomedi participated studies Pennington clinical in have at cohortcal. This established was better to characterize the subjects follow of the population and to time for health over of a varietythe development problems. of health study This holds of providing important the promise on the information activity,role of physical nutrition, obesity lifestyle and other behaviors on the future of the population, particularly health in Louisiana. It is clear that physical activity physical that clear It is obesity and intricately are conditions. health with numerous associations their in linked about questions remain the shape of the dose-reSeveral We are actively projects are onseveral collaborating We designed to the Cana including unlock the questions answers above, to erate or vigorous physical activity, and how much and which activity, or and which vigorous much physical and how erate types activity of physical should be recommended for optimal benefits.health much Beyond thesequestions, have basic we learn aboutto differences gender ethnic and activ physical in health. and obesity, ity, Peter T. Katzmarzyk,Peter T. Ph.D., FACSM Chair in Nutrition, Associate LPFA Professor, Executive Director for Population Science Physical Activity Obesity and Laboratory Epidemiology Physical 72

POPULATION SCIENCE Walking Behavior Laboratory Associate Professor Associate Catrine Tudor-Locke, Ph.D. tes, health. andoverall cardiometabolic diabe composition, body including outcomes, health important timespent atcadence differentlevelsrelationship and between year, Inthisphysical activity. next we investigating will be the monitored objectively on based reasonableeasewith in data sets identified andrunningbe discriminated can walking, be from strategy. can Puttering asimple pattern-recognition represents conditions free-living under sity, ofcadence thestudy andso intensity. and inten ofwalking Cadence increases speed with day, per steps with and together alone at as ameans ofgetting (steps cadence minute), ofminute-by-minute per of patterns year,past we have to theassessment on focus begun andanalysis include an elementofintensity in their recommendations. Inthe intensity of movement. Physical guidelines worldwide activity of adescription lacking for volume, criticized has andit been Steps day per is ameasurement representing physical activity healthrelation outcomes. to several important research to also answer “how many day per steps are too few?” in our expanding we are lifestyle, of an sedentary overly effects cial thegrowing awareness With populations. ofthedetrimental enough?” in children/adolescents, adults/spe and adults, older “how on many dayconsensus focused per steps are articles weexample, have international ofthree thewriting recently led today. captureper steps accelerometers andpedometers For using behaviors monitoring ofwalking in theobjective leader as aworld itself has established WalkingThe Behavior Laboratory mobility. ofpersonal all of almost types aspect and afunctional ofmany nonautomated apart chores, transportation, personal ofhuman-powered ity. form is walking theforemost Inaddition, physical leisure-time activ reported sistently frequently themost to measure andpromote.important Walking exercise for is con alsobut any lower limb locomotion) thesingle most is probably (most obviously walking, activity ambulatory behaviors, activity ofphysical all types Of across thelife span is strong. lifestyle physically ofa active thehealth benefits science supporting The important healthimportant outcomes. in relationment behaviors ofandmotivationto walking for FOCUS SCIENTIFIC REPORT The goal of this laboratory is to study themeasure is to ofthis study goal : The laboratory

2 010–2011 ------National InstitutesNational Health. of Agriculture, of the and Association, Department the U.S. can Heart Research by grants this in the isfrom Ameri supported laboratory at riskpopulation increased ofcardiovascular disease. relatedpressure in health a topublic messages improving blood pressure. WALKMORE elevatedwomen with will blood inform day per steps +cadence) in overweight/obese, postmenopausal accumulating on dayfocused per steps more andtheother on interventions walking (one pedometer-based oftwo impacts oftherelative ourWALKMORE study awardsociation to support conditions. We As have Heart recently an received American in free-living ofwalking estimate expenditure energy to better andchildren in order adults, older in adults, expenditure energy toies elucidatecadences different and therelationship between gait lengths, stride speed, and cadence. We are conducting stud participants’ research capture that us allows to instantaneously walkway measurement includinged an electronic technologies We original research studies. have to funding support adopt seek and sets, data into existing tap methods, qualitative and titative To achieve thelab’s mission, ofquan werange employ abroad et al., 2009. al., et Tudor-Locke 2004; Tudor-Locke and 2008; al., Tudor-Locke et Bassett, from and Adapted Graduated Step Index. PENNINGTON RESEARCH CENTER BIOMEDICAL Tudor-Locke,Catrine Ph.D. Faculty: - - - - - POPULATION SCIENCE 73 is the fourth 010–2011 2

The 2011 installment of of installment The 2011 ReportLouisiana’s Card Activityon Physical for Children and Health and Youth annual edition of the Report is which Card, guided a Research by Advisory Committee members of composed state. the from across SCIENTIFIC REPORT SCIENTIFIC PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON DIVISION OF EDUCATION

investigate genetic, molecular, behavioral and population THE DIVISION OF EDUCATION administers aspects of obesity. The other grant from the National Center programs in three major areas: (1) training the next generation of Complementary and Alternative Medicine provides training of scientists, (2) producing scientific symposia that focus on in research skills to identify new plant compounds that may Pennington Biomedical’s research efforts and attract world-re- impact metabolic syndrome and its related disorders includ- nowned scientists to our Center and (3) organizing profession- ing diabetes. In addition to research collaboration with faculty al and community education programs to engage the citizens mentors, postdoctoral fellows also attend graduate nutrition of Louisiana and the state’s medical community, as well as to seminars, participate in workshops on grant proposal writing, provide educational outreach to the general public. enjoy presentations by Center faculty and visiting scientists, Postdoctoral Training Programs and participate in responsible conduct of research seminars and The Division directs training programs for Pennington Biomedi- data presentation sessions. cal’s postdoctoral fellows. These programs are designed to Predoctoral Training train fellows to become productive research scientists capable of establishing independent scientific careers in biomedical Students from Louisiana universities and medical schools research. Many of our fellows are sponsored by NIH Institu- receive hands on experience in research and laboratory skills tional Postdoctoral Training Grants. One of these grants is each year at Pennington Biomedical, and the Division assists in funded by the National Institute of Diabetes and Digestive the placement, training, and tracking of student trainees. The and Kidney Diseases and trains fellows to conduct studies that Division of Education recently teamed with LSU Health Sciences

74 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER Center in New Orleans to address the growing need for physi- LSU Agricultural Center cian scientists by jointly acquiring an NIH Institutional Training The Division of Education continues its successful partnership Grant. This grant will allow Pennington Biomedical to provide with the LSU Agricultural Center and its Division of Education, the summer research experience to medical students interested in Louisiana Cooperative Extension Service. The objective of the part- careers in biomedical research focused in the areas of diabe- nership is to provide an effective, efficient means of disseminating tes, obesity and metabolic diseases. In addition to exposure to the latest health and nutrition the activities of Pennington Biomedical research laboratories, information to the people of students receive mentoring from accomplished Pennington Louisiana. PowerPoint presenta- scientists, and receive didactic training in research methods, tions and booklets suitable for ethics, and scientific communication skills. use by extension agents, school DIVISION OF EDUCATION Scientific Symposia Series teachers and the public are contained in the “Pennington The Division of Education collaborates with Center scientists Nutrition Series,” a downloadable to organize symposia designed to help direct the scientific collection on the LSU Ag Center focus of the Center. These conferences draw top international and Pennington Biomedical scientists to Baton Rouge and allow them direct exposure to Research Center web sites. the research efforts of the Pennington Biomedical Research Phillip J. Brantley, Ph.D. Center. As many as 30 visiting scientists join together with LSU Health Care Services Director of Education; John S. McIlhenny Pennington Biomedical scientists at each meeting to pres- Overweight and obesity are Endowed Professor ent data and develop conclusions and recommendations for extremely prevalent among pa- future research on current hot topics in science. Meeting tients attending public teaching proceedings and conclusions are published on the Center web hospitals and their affiliated site and in scientific journals. Recent topics have included: medical clinics in Louisiana. Adiposity in Children and Adolescents: Correlates and Clini- The Division of Education part- cal Consequences of Fat Stored in Specific Body Depots; ners with the LSU Health Care Bariatric Surgery: Do the Mechanisms Hold the Key for Novel Services Division to provide Therapies; and Imaging in Translational Research. weight management instruc- Professional Enrichment/Community Education tion to the faculty and staff of the state’s public medical clinics. This initiative includes training in proven, low cost patient- To promote healthy lifestyles and heighten awareness of directed weight management techniques, counseling methods chronic disease risks, the Division of Education organizes public and program evaluation. events focusing on educational outreach. Two popular annual events include the Irene W. Pennington Wellness Day for Future Goals Women and the Men’s Health Conference sponsored by the In order to promote the training of future biomedical scientists, the Di- Louisiana Men’s Health Organization. These events alone attract vision of Education plans to expand programs for predoctoral trainees, over 1,500 participants and provide them with access to semi- expand our role in medical education to promote the next generation nars, health screenings and exhibits at no charge. of physician scientists, and to reach out to younger academically gifted The Division of Education also provides administrative support Louisiana students in hopes of encouraging them to consider careers for community programs organized by other faculty including in biomedical research. We will achieve these new initiatives while the Public Forums on Dementia Research series, the annual enhancing our existing training opportunities for postdoctoral fellows Childhood Obesity Conference, and community seminars on the and continuing to produce high quality scientific symposia despite health benefits of botanicals. These events provide an excellent the growing challenge of acquiring the necessary funding for such en- opportunity for recruiting study participants and allow Pen- deavors. We will continue to serve the citizens of Louisiana by alerting nington Biomedical to directly contribute to the well-being of them to new discoveries of Pennington Biomedical and offering new the Greater Baton Rouge community. opportunities to participate in the continued success of our Center.

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 75 CORE SERVICES

76 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER CORE SERVICES 77 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC ingestive behavior assessments, imaging procedures using both both proceduresingestive assessments, imaging behavior using MRI assessments and of metabolism and DXA metabolic the in chambers. provide researchers The with Cores Science Basic cutting to edgeaccess technology and very focused technical furtherprocedures to interests. research services their Basic culture cell and tissues, of cells and imaging include microscopy facilities, metabolism animal comparative biology, and animal services Additional of genomics, include the area behavior. proteomics and metabolomics, transgenics. as well The as Science are directed Cores Population providing at researchers statistical to and database expertise.with access addition, In interlibrary the Library offers reference, Center and Information loan and bibliographic processing, instruction among other services.information -

full support research for clinical

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON OVERVIEW OF OVERVIEW

The Pennington Biomedical Research Center provides its Biomedical Center Research The Pennington researchers with state-of-the-art services core designed to ofandtimeliness precision vital technical efficiency, improve procedures needed across research boundaries. services Core basic, and population sci clinical, divided three areas: are into CORE SERVICES CORE ence. The Clinical Cores provide Cores The Clinical ence. studies IRB beginning development, with protocol and trials submission, and budgeting and contract assistance. Clinical services biological include participant recruitment, phlebotomy, specimen collection, processing and analysis, exercise testing, dietary and psychological assessment, The assessment. Center for trials options and offersoutpatient also inpatient both performed services clinical Additional here. include preparation the metabolic by of meals kitchen, collection data and storage medicalin medication records, preparation the pharmacy, in 78 CORE SERVICES Animal andBehavior Metabolism Kidney Diseases.Kidney by Institute the National funded Digestive and and Diabetes of This Research core by the Center Nutrition is Obesity supported well astraining provide in using theequipment. data,as investigators andanalyzing in designing experiments conditioninging, and operant (Skinner boxes). assist Core staff (Pavlovian) classical memory, and learning spatial condition measuringfor cognition includevariousassessing mazesfor include hunger, andanxiety. apparatuses Specialized thirst, measures Motivational endurance. and balance, gait, tion, includelocomo Measuresequipment. ofmotor performance is alsoof behavioral tests available using designed specially using machinesmall for an NMR designed animals. Abattery (fatposition andlean content) measured noninvasively be can (circadian periods daily across rhythms). com Body frequency measured to be can estimate bout meal size andfeeding figure). Usingbehavior other automated feeding apparatuses, (see system ous monitoring24 for animals in anewstate-of-art to this simultane provide technology andexpand to upgrade andwater intake. and food This year,activity thecore was able bohydrate metabolism) fat vs. over time, as well as locomotor quotient (car andrespiratory estimates expenditure ofenergy dioxide to generate andcarbon of theexchange ofoxygen highly specialized equipment provides continuous monitoring For measuring metabolism, rodents. oflaboratory acteristics andbehavioral char ofmetabolic totors range assess awide investiga for This core thetools provides andtechnical support Professor Ph.D. Ingram, K. Donald models. assessment andbehavior in small ofmetabolism animal MISSION SCIENTIFIC REPORT : The missionThe ofthis core is to facilitate the

| Jennifer Dowden, M.S. Staff: Ph.D. Ingram, K. Donald Director:

2 010–2011 in small animals. whole-body metabolism measuring for Apparatus ------Biostatistics andData Management Professor D.William Ph.D. Johnson, extramural researchers. researchers. extramural allowsparadigm the team to intramural both work with and non-Pennington data. This development and data storage that sets may data bothspecific contain Pennington data and creation ofstudy- custom applications expedited for oped data access. team The has desktop devel Web-based via sets data investigators andprovides study-specific study; with and analysis; monitors the data processing throughout each storage filesfor to electronic observation ofdatafer from to researchers with ensure theefficientand accuratetrans to theclinical research team database. The interfaces portal Web-based is thecontinuing development ofaproprietary responsibility primary Their facility. data-coordinating cal as dataacomprehensive managementThe team clini serves relevant to thePennington mission. Biomedical sue independent research and methods in statistical theory to pur and continuing education through expertise current to encouraged maintain is strongly faculty The Sciences. cal ofresearch Population, in Basic, integrity andClinistatistical to ensurerigorous expertise team offers biostatistics The data. in theobserved captured interpretation ofresearch findings that theobjective for are statisticaloutcomes techniques and provide state-of-the-art that databases accurately researchcreate describe electronic overarching Our presentations. ysis, is andsummary goal to anal statistical management, data quality-controlled through formulation design toand execution study efficientresearch laborations that leadto hypothesis transition asmooth from col andData Management Core seeks Biostatistics The Biomedical Research Center.Biomedical at Pennington research objective and enhance reliable andresources to anddata management expertise tistics MISSION PENNINGTON RESEARCH CENTER BIOMEDICAL : The mission The ofthis core is to biosta provide Ruth, B.S.; Vy Nguyen, B.S. B.S. Nguyen, Vy B.S.; Ruth, John M.S.; Stewart, Aimee’ M.S.; Arnold, Jessica B.S.; Murla, Connie Staff: Management Data M.P.H.; Wenting M.S. Xie, McGlone, Meghan M.S.; Han, Hongmei Staff: Biostatistics Ph.D. D.William Johnson, Director: ------CORE SERVICES 79 - - - - 010–2011 2

incubators, a liquid 2 Director: Jeffrey M. Gimble, M.D.,Ph.D. Staff: B.S. Shah, Forum SCIENTIFIC REPORT SCIENTIFIC The mission of this core is to provide cell culture culture provide cell to is core of this The mission : To provide more efficiency, the Cell Culture Core has provide more efficiency, has Core Culture To the Cell

MISSION facilities, equipment, and expertise requir investigators to services.ing such * recently merged with the Cell Biology and Cell Imaging merged Biologyrecently Imaging with the Cell and Cell sole direction the under now is core The combined Core. Burk. David of Dr. The Cell Culture Core is equipped is Core with four certified Culture The Cell Biologi cal Safety Cabinets,CO four humidified tion, freezers). All equipment is operatedequipment is withAll emergency freezers). tion, A closed failure. room of power backup the event power in viral transduction for any or radioactive available is isotope requirefurther studies would that The isolation. facility is avail and is environment access a controlled in maintained nitrogen dewar for cell samplenitrogen for dewar cell cryopreservation, phase and with capture capability, image microscopy contrast refrigera centrifuges, balance, support (pH meter, equipment Biomedical and outside laboratories Pennington all able to on staff a fee-for-service entities Core Culture The basis. Cell andmaintains monitors the instruments on a regular basis accuracy their experiments. culture for for insure use cell to with investigators assist to available In addition, the staffis experimental culture questions regarding cell and design any conduct. The facility hours and per open day is for 24 use on prospective weekends. All users are required undergo to certificationin blood-borne pathogen safety prior regulations specific in authorizationto for entry and operation. Training or processes techniques can culture be providedcell on “as an requested” basis. Jeffrey M. Gimble, M.D.,Ph.D. Professor Cell Culture Cell - - - - - Director: David Burk, Ph.D. Staff: Marilyn Dietrich, M.S.; Shirley Ennis, B.S.,Drury HT (ASCP); Ingram, B.S.; Williams, B.S.D’Andreas The mission of this core is to provide state-of- to is core of this : The mission concentration. concentration. 2 PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON MISSION ed instrumentation and support collection data facilitate to Biomedical and future current Pennington by and analysis staff. and their PIs the-art and flow histological-, bioimaging-, cytometry-relat This core is supported by the Center of Biomedical Research Excel of Biomedical Research supported is Center the by core This The analytical/flow cytometry sectionincludesfluorometric a equipped reader plate with a robotic fluidic system for ad of the platforms on almost all available the within is Training thein development CBBC,assistance foravailable staff and are of experimental collection, data and in and protocols analysis, interpretation. chemical stains. In addition, the core provides access to a laser a laser to chemical stains. provides access In addition, the core microdissection system for precise the collection cells of single fromor whole sectioned tissues materials. formats, vanced kinetic a four-color plate studies multiwell in analyticalhigh-speedand a flow cell sortercytometer, housed hood. flow lamellar a BSLII in funded the by Obesity and Nutrition Center the lence Research of Health. National Institutes The histology/specimen preparation sectionThe houses histology/specimen of the core the necessaryall sectioning, processing, equipment for tissue and staining Biomedical needed researchers. Pennington by processor, tissue automated an to The CBBC provides access cryostats, a vibratome, rotary microtome, a sliding microtomes, and autostainers for both stains traditional and immunohisto David Burk, Ph.D. David Instructor Cell Biology and Cell Imaging Cell and Biology Cell orescent imaging, live cell imaging, ratiometric imaging, total ratiometric total imaging, imaging, cell live imaging, orescent reflectioninternal fluorescence (TIRF) and techniques, whole scanning. Theslide confocal system, the TIRF platform, and one epi-fluorescence systemare capablelive-cell of imaging of proper temperature the maintenance involving techniques and CO The Cell Biology and Cell Imaging Core (CBBC) is loosely is divided (CBBC) Biology Core The Imaging Cell and Cell three sections—imaging, preparation, into histology/specimen and analytical/flow cytometry. Theimaging section includes the necessary two-photon platforms for confocal microscopy, brightfield imaging, 3D epi-fluimaging, confocal microscopy, 80 CORE SERVICES Clinical Chemistry and private sponsors. numerous public Defense, of and Health, Department the U.S. This core by grant(s) is supported Institutes the from National of antioxidant andhigh-molecular-weight status, adiponectin. glutathione,oxidized cadmium, glutathione peroxidase, total include the following: superoxide dismutase, reduced and novel biomarkers. New assays developed during 2010-2011 continues laboratory The to provide development method for Examiners. Medical by the Louisiana are certified phlebotomists ofStateBoard and technologists All medical program. lipid standardization Institute Lung, andBlood and Prevention–National Heart, in theCenters also Disease Control participates for laboratory The Pathologists. ministration ofAmerican and theCollege HealthCareby the Financing andisAd certified practices control assurance follows quality rigorous laboratory The ing clients. Research Institute ofEnvironmental andcontract Medicine, clinical trials, basicassays research, Army totheU.S. support than more 275 core performs The different clinical performed. year,past assays and350,000 were venipunctures over 5,000 the During anddata. ofspecimens management system long-term specimen storage and maintain a comprehensive testing.We chemistry,special andpoint-of-care also provide phlebotomy, accessioning, chemistry, hematology, urinalysis, in thefollowing areas: core services provides The teric tests. assays routinescreening to eso highly from ranging complex a Corecomprehensive offers menu Clinical The test Chemistry Professor Ph.D. Rood, C. Jennifer research. nutritional through wellness and health promoting while foster aclimate andprofessional achievement ofpersonal methodology, accurate provide and results, test andtimely MISSION SCIENTIFIC REPORT : The mission The ofthis core is to develop innovative Lewis; Lisa Jones; Sharon Thomas Sharon Jones; Lewis; Lisa PatrickB.S.; Cotogno, Carlo Milo; B.S.; Donald Steven Naremore, B.S.; Clark, April B.S.; Loftis, (ASCP); Holloway, (ASCP); MT Laura Amanda Tuminello, Conner, (ASCP); MT MT Bridget Graves, MT (ASCP);Margaret Jamie (ASCP); MT (ASCP); MT Lee, Kimmel, Carla Staff: DABCC, Ph.D., FACB Rood, C. Jennifer Director:

2 Stacey Roussel, MT (ASCP); MT Stacey Roussel, Stephen 010–2011 - - - Associate Professor Associate DACLAM Ph.D., DVM, Robert, Barry Comparative Biology sociation for Laboratory Animal Science. Laboratory for sociation materials course on As bybased theAmerican developed animal technicians care training laboratory for isOngoing animal biomethodology, andoccupational health andsafety. including institutional andgovernmental laboratory policies, animal anduse training care covering oftopics range abroad animals attend must All laboratory work with employees who laws currenton toall regulations.and applicable keep staff Training toanimal is vital ensure and care quality thehighest use. and member, animal reviews approve andmust care all laboratory community a and veterinarian, a scientists, of composed Biomedical’s Institutional Animal Careand Use Committee, animals. Pennington anduse oflaboratory care theproper for Regulationsand ofAgriculture Department and theU.S. mals Service PolicyonHumane Care and Use of Laboratory Ani complies theNational with Institutes ofHealth and endorses CBC fully The animal anduse program. care tory highlighting labora ourcommitment to quality thehighest animal care, laboratory for the“goldrepresents standard” Animal International Care and Accreditation ofLaboratory CBC’sThe Assessment for accreditation by theAssociation Pennington scientists. Biomedical use laboratoriesby for testing, surgical, preparatory anddiet behavioral animal procedural, and facilities quarantine and animal housing, receiving, unit providinglaboratory service Core Comparative (CBC)The Biology is a38,000-square-foot tists usinganimal models. tists scien for care, training,veterinary andtechnical support animal housing quality space,est and animal husbandry MISSION PENNINGTON RESEARCH CENTER BIOMEDICAL , The Guidethe for The Care andUse Animals, Laboratory of : The missionThe ofthis core is to thehigh provide . These documents. define These our responsibility Sterba, ALAT;Sterba, Terry, Kayla B.S. Shannon Simmons; Monique Rageur,Julia B.S.; B.S.; Payne, Sarah Ozoral; Suna M.S.; Mercedes, Frank Hsu; Faye ALAT; Louviere, Marleny LAT; Chase, Paula Grimes; Linda Dry; Michelle Staff: RVT, RLATG, CPM Kloster, B.S., Cindy Director: Assistant D.V.M., DACLAM Ph.D., Robert, Barry Director: Cynthia ALAT; Angelloz, Tracy Brown; Animal Welfare Act Public Health - - - - - CORE SERVICES 81 -

- - - - TM - 010–2011 2

Leanne M. Redman, Ph.D. Assistant Professor SCIENTIFIC REPORT SCIENTIFIC The mission of this core is to perform to is core of this : The reliable mission Crystal Traylor, N.P.; Elizabeth Frost, B.S.; N.P.; Crystal Jonathan Traylor, MISSION Staff: M.S. Clement, T. B.S.; Tyler Simmons, and reproducible measurements of energy expenditure and indirect using humans in calorimetry. oxidation substrate Eric Ravussin, Ph.D., Director Douglas L. GordonProfessor, Chair in Diabetesand Metabolism ture. We have 13 portable bedside 13 metabolic carts have We ture. (Deltatrac II tion, and measurement quality and measurement tion, control. The utilizes indirect core calorimetry energy measure to expendi man provide scientific oversight of all assessments, instrumenta all of oversight provide scientific man The overseen operations Energy are daily Metabolism by Core Red and Leanne Drs. whereas Ravussin Eric N.P., Crystal Traylor, and MAX-II) service, in of energy for measurements allow which conditions. resting under oxidation substrate expenditure and and chronic acute measure can also these we devices, Using energy in changes thermogenic metabolism response in to the response as to well as temperature, and ambient compounds dietaryvarious treatments. and exercise Biomedical two has currently whole-roomPennington calorim eters, or metabolic chambers. The room calorimeters used are to components hours, as well as energyassess expenditure 24 over of energy expenditure sleeping as sponta such metabolic rate, activity, Theneous meals. physical and the thermic response to softwarecustomized platform outputs minute-by-minute data effects. of acute for the assessment allowing also chamber Each designed L and was to of 27,000 volume approximate an has for studyprovide a pleasant ambiance participants. undergo the Energy Metabolism will Core the nextWithin year, house a to new space acquire will We renovations. substantial two and metabolic room additional suite calorimeters, rate one with the L) (10,000 L and one smaller of 27,000 size with a similar capacity modify for to allow will which temperature, ambient studies. be will environmental work This conducted mostly by our Biomedical Clement, Engineer. Tyler Mr. body of core provides tem measurement the core Additionally, perature, heart variability, and rate skin temperature infrared by metabolic cart 750 and measurements Approximately imaging. metabolic performed are chamber measurements 150 annually Energy the Metabolismby Core. of Health, supported from National is Institutes grant by core This sponsors. industry and Agriculture, of Department U.S. Energy Metabolism Energy - - - - - Director: Catherine M. Champagne, Ph.D., R.D. Faculty: H. Raymond Allen, Ph.D. Staff: B.S.;Dawn R. Mary Turner, Marlene B.S.Afton, Several research protocols use the the use protocols research Several Some studies collect food frequency The mission of this core is to provide accurate provide accurate to is core of this The mission : medical, using a unique recipe a unique systemmedical, calculation using USDA Food and Nutrient Database for Dietary Studies 4.1 Food and Nutrient Database for DietaryUSDA 4.1 Studies (August 2010) Supplementary or fromliterature information the scientific other reliable food composition tables User-defined foods,data for nutrient theinput of allowing foods needed or recipes menus in appropriate for an which be cannot food found otherwisematch Recipes Bio users input by of the system Pennington at USDA Nutrient Database for Standard Reference, Release 23 Reference, Nutrient Database for Standard USDA 2010) (September PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON MISSION • • • • • information on dietaryinformation study of research partici intakes pants who food keep food records, frequency question dietary menus designs also and/or naires, recalls. core This targets. nutrient specific meeting Catherine M. Champagne, Ph.D., R.D. Professor Food Frequencies. Food tion files to analyzeto tion files dietary 2009-individuals. In of intakes 20,406 on of data dietary lines processed. were intake 2010, a longer period over capture intakes to questionnaires (FFQs) a scannable questionnaire, to converted Currently of time. being is the Block Food Frequency Questionnaire (1992) used, with results exported electronically; approximately processed were during the past FFQs two years. Online 1,085 (NCI) Institute Cancer National and current of this versions development. in are questionnaires Food Diary food the MENu utilizes which Program, composi Food Diary Program. Diary Program. Food tor Margaret C. Margaret tor Moore. Primary sets data used from are the of The count total U.S. Department (USDA). of Agriculture food files foods composition and recipes MENu’s in contained from sources: data the following numbers 27,107, Pennington Biomedical’s nutrient analysis system is Moore’s system Moore’s is analysis nutrient Biomedical’s Pennington named afterExtended its benefac Nutrient Database (MENu), Dietary Assessment and Food Analysis Food and Assessment Dietary 82 CORE SERVICES Exercise Testing Associate Professor Associate Conrad Ph.D. Earnest, testing and exercise performance. exercise and testing muscle biopsiesin associationexercise with to perform ability musculoskeletal jointin most areas. In2009, the we added endurance resistance for ajoint’s throughout ofmotion range tocomputer measure microprocessors torque, power, and with 0to interfaces 1000W. from ranging system Biodex The increments ofsingular is ergometer capable wattage Sport ing patient kg weights up to (750 340 lb). Excalibur Lode The negative/downhill (-10%) accommodat while grades running mph) positive ofimposing both (+25%) andis capable and (0.124 km/h to 40 0.2 from ranging tion capabilities to 24.8 ValiantOur Treadmill andaccelera speed has azero starting constant strength testing velocity perform to dynamometer strength isokinetic also aBiodex includes ergometer. bicycle core The Sport face, Excalibur andaLode (200 long cm; 78.7 inches)and extra treadmill running sur Valiant (105 treadmill a“double-wide” with cm; 41.3 inches) cart for the performance ofVO theperformance for cart TrueOne® aParvoMedics with metabolic core isThe equipped oftheir studies. needs mentation oftesting protocols facilitate thetesting to best the core is to assist investigators thedesign with andimple of goal oftheir research The populations. capacity strength and tigators wish thecardiorespiratory who to characterize Testing Exercise The ofclinical inves theneeds Core- serves ical employees. ical of ourCorporate Wellness Pennington for Program Biomed exercise exercise interventionsportion specific for the and accurateprovide requiring exercise monitoringstudies for aim oftheFitness Center primary is The to performance. spiratory, ofexercise parameters andmuscular strength precise, andreliable assessment cardiore ofphysiologic, : The mission: The ofthis core is to accurate, provide MISSION SCIENTIFIC REPORT

Melissa Lupo, B.S. Lupo, Melissa Staff: Conrad Earnest, Ph.D., FACSM Director: |

2 2 010–2011 max testing, acustom Lodemax ------Genomics Associate Professor Associate Ph.D. Salbaum, J. Michael This core by theInstitutes National is supported Health. of experiments. high-throughput handlingfor liquid robotic Coulter andaPerkinElmer support MultiPROBE® FX II, Biomek quantitative Two aBeckman PCR. robots, perform pipetting are available to array blocks well, well, 384 andlow-density 96 with equipped systems detection sequence 7900HT Biosystems fourApplied detection, nucleic acidfragment 3130xl Biosystems Applied two on For analyzers. genetic analysisClassical andfragment is sequencing performed DNA andAcumenta Literature Lab™ Genomics, software. JMP® GeneSifter®, includeIluminaforms GenomeStudio, Geospiza plat thehigh-throughput tools dataon generated to analyze using theIlluminaprovided BeadArray scanner. Bioinformatics is service Microarray Geospiza. with through acontract able is avail support Bioinformatics equipment. and auxiliary Technologies 5500xl SOLiD™ platform Biosystems Applied (ChIP)chromatin immunoprecipitation analysis using Life a methylation, profiling, small andexpression RNAdetection, vices including genomic DNA and exome sequencing, gene Coresequencing Genomics The ser next-generation offers the biomedical research community ofLouisiana. research community the biomedical and protein to investigators at Pennington and Biomedical qualitative for and quantitativeservices RNA, analysis ofDNA, and instrumentation, training, consultation, expertise, art state-of-the- providingby researchproduction data effective MISSION PENNINGTON RESEARCH CENTER BIOMEDICAL This core seeks to achieve high-quality and cost- to achieve: Thishigh-quality core seeks Genomics Core equipment and data generation. Carmouche B.S.; Diana Holmes, M.S. Holmes, Diana B.S.; Carmouche Richard B.S.; Newman, Susan Staff: Professor Associate Ph.D., Salbaum, J. Michael Director: - - - CORE SERVICES 83 ------

010–2011 2

Crystal Traylor, R.N., A.N.P.-W.H.N.P.; A.N.P.-W.H.N.P.; R.N., Traylor, Crystal Director: William Cefalu, M.D. Staff: Cook, Pam L.P.N.; Braymer, R.N.,Amy Kim Crotwell, Lisa Dalfrey, L.P.N.; F.N.P.; RhondaL.P.N.; Stephanie Hilliard, L.P.N.; Valerie L.P.N.; R.N.; Taylor, Bridget Tatum, Celeste L.P.N.; Waguespack,Toups, R.N.; Linda RA; Jones; Sereda, Gloria Olga LPN Parker, Stockton; LaTonya SCIENTIFIC REPORT SCIENTIFIC

The Inpatient Clinic serves Clinic The Inpatient needs the of clini : cose tolerance testing, pharmacokinetic testing, studies, tolerance cose and other proceduresrelated Dedicated biopsy room and skeletal for adipose tissue biopsies muscle clinicalSatellite chemistry sample-processing and -acces dows, private bath facilities, and telephones and facilities, bath private dows, hyperinsulinemic dedicated rooms for euglycemic Two clamps IV glu testing, roomProcedure tolerance for oral glucose room sioning of food and Room measurement the intake dedicated to selectionmacronutrient equipped Clinic/EatingFully Inpatient Laboratory Metabolic Kitchen TV/ where can for volunteers they watch Lounge/sunroom surfDVDs, admitted games while and play the Internet, to for specific the inpatient protocols research Large pharmacy, nursing includes a remote station that table work and access, Internet/intranet Psychology collection data for comple questionnaire area tion on PCs Seven rooms, with two beds each, for overnight clinical rooms, with clinical Seven two beds for overnight each, been recently stays these and procedures; rooms have comfortably and are renovated furnished win with large MISSION • • • • • • • • • cal investigators for the conduct end cal of advanced investigators clinical points studies metabolism. and diabetes, in of obesity, • py (MRS), and pulmonary function (MRS), py units testing and exercise week. a days 7 24 hours staffedTheday, a is unit sound, 3T magnetic resonance imaging (MRI) and spectrosco and (MRI) imaging resonance magnetic sound, 3T Immediately adjacent facilities: DXA, echocardiography, ultra DXA, facilities: echocardiography, adjacent Immediately The of: consists unit Inpatient Clinic Inpatient William Cefalu, M.D. Douglas L. Manship Sr. Diabetes in Professorship ------Director: B. Heymsfield,Steven M.D. Staff: M.S.;Kori Murray, Randell Deen, Blanca (R); Desharnais, R.T. R.T. Brittany B.S.;(R); Inlow, Kimberly Julia Amant, St. Landry, R.C.T.; R.T. (R) without biopsies: without for whole-body resonance magnetic nuclear in situ TM : The provides state-of-the core art imaging -based coordination of multicenter DXA studies DXA -based of multicenter coordination TM Hologic® QDR-4500A ab energy and GE dual iDXA X-ray of whole-body for the measurement sorptiometers (DXA) density site-specificmineral and bone composition Oasis An EchoMRI (NMR) body composition (NMR) ultrasound/Doppler for cardiac SSA-280 Aplio A Toshiba and and general purpose ultrasonography, carotid imaging, studies reactive artery of post-ischemic (brachial hyperemia flow-mediated dilatation) of peripheral for measurement arterial tone EndoPAT2000 An a 64-slice to (CT) tomography computed Access scanner at Rougethe Baton General Bluebonnet Medical campus Center Assistance with special projects requiring image analysis or ultrasound imaging, images, resonance magnetic of CT, including clinical multicenter trials ercise phosphocreatineercise recovery and maximal (resting rates synthesis) ATP fMRI with appropriate stimulation visual paradigms Proton spectroscopy of intrahepatic Proton for the measurement lipidsand intramyocellular Phosphorus spectroscopy post-ischemic measure and ex to PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON MISSION cal investigators who needcal high-quality investigators of body measures function. and composition • • instrumentation which allows turnkey solutions for clini turnkey allows instrumentation which • • • • • • • • Steven B.Steven Heymsfield, M.D. Executive Director Imaging/MRS This core places in the hands of researchers a variety the hands in places core This of instru mentation and technical services and technical mentation following: the including The core also includes a magnetic resonance imaging (MRI) and (MRI) imaging resonance includes a magnetic The also core spectroscopy and functional (MRS) The con lab MRI lab. (fMRI) a series of special MR scanner, EXCITE Signa® sists of a GE 3.0T ized coils and instrumentation for MRSized coils and fMRI, and clinical In comfort patient and convenience. toward facilities oriented a varietyaddition to of body instrument al scans, image this complement is rates turnover of restingThe ATP measurement researchers allows technique This ed optical spectroscopy. by directly muscle. skeletal to in oxygen measure consumption mitochon the rates, turnover ATP resting with combined When can be directly for the first measured or P/O ratio coupling drial intacttime in muscle. lows researchers to noninvasively make the following measures measures the following make noninvasively researcherslows to of biochemistry 84 CORE SERVICES Library andInformation CenterLibrary graphs are being organized and added as they become available. as they become organized andadded graphs are being and print archive andphoto is in progress; newdocuments Faculty Publicationexisting into Database acomplete electronic Developmentofthe presentations and other signage as needed. research for poster printing service acustom poster provides also exchange. library The paperback andancheckout active emerging technologies. They include a laptop/electronic reader with several that newprograms combine traditional services andInformation Center Pennington The now Library offers scanners) ofsoftware. printers, as welland b&w as avariety hardware with (color offourcomputercomprised workstations and a computerWireless connectivity lab are also available, accessibleand other programs through thePBRC network. resources to these for in addition EndNote, Reference Manager and support provides instruction staff (via EbscoHost.) Library line Full-Text, Science PsychInfo, Abstracts andSocial Biological (via Index ISI), andAgricola, Reports Citation and Journal Med Science/Artsthe Science/Social &Humanities Indexes Citation (via includeMedline andEbscoHost), databases PubMed Library Network. LOUISand Louisiana Library ofMedicine ofLibraries oftheNational Network ing member is arenew library The methods. delivery various document informationalapproximately items requested through 2500 annually provides thelibrary toaddition this collection, concentrated In provided. field is incollection themedical resource Access a week. to print andelectronic aspecialized aday, hours and is accessible to twenty-four them seven days to all employees instruction processing, andbibliographic loan interlibrary toues offer reference, informationservices, contin library the librarian, assistant and director a by Staffed health information resources andservices. by providinglimited community toaccess thelocal to services provides also Pennington andstaff. Center Library The faculty tosigned anticipate to ofall andrespond theinformation needs andInformationare de Center PenningtonThe services Library Director AHIP MLIS, BA, Steib, Lori develop learning, teaching, and research at the Center. andaccess to to support theinformation resources needed faculty, staff,providingBatonby and the Rouge community Center thePennington students, is to Biomedical serve MISSION SCIENTIFIC REPORT :

The mission of the Library andInformation missionThe oftheLibrary

Marilyn Hammond, B.A. Staff: AHIP MLIS, Steib,Lori BA, Director: 2 010–2011 - - - - - Mass Spectrometry Mass Professor Ph.D. Rood, C. Jennifer numerous public and private sponsors. Defense, of and Institutestional Health, Department of the U.S. Research by thisgrant(s) in is funded laboratory the from Na in liver,lipogenesis tissue, adipose andmuscle. to glucose andmethods assessnovo andurine de yl in serum theanalysis for method of3-O-meth phy/mass spectrometry year duringthepast includeagas chromatogradeveloped during euglycemic hyperinsulinemic clamps.techniques New measure 6,6-D2-glucose to determine hepatic glucose output to is used other conditions.currently being This technology ofobesity, indiabetes, and studies ofmetabolism the role acids to explore carbohydrates, aminolabeled acids, andfatty to are used measure instruments stably These detectors. etry massspectrom with Agilent chromatographs gas has three glycolysis. thecore Inaddition, andwhole-body expenditure, are to also used measurewater, total body total daily energy instruments The themeasurementfor expenditure. ofenergy relation to thecommon isotopes, isotopes, such as Oxygen-18of heavy andHydrogen-2, in we accurately can ments, measure andprecisely theamount instru these ofdeuterium. With preparation thesample for Oxygen-18 Hdevices andthree preparation, are used sample Twoto themass spectrometers. for are used gas benches toaddition automated devices preparation interfaced sample in core has The dren. five ratio isotope mass spectrometers, andchil in adults to used hazards ourvolunteers be andcan nolism. they pose isotopes Sinceare nonradioactive, stable atoms, human to metabo as tracers are heavy study or used Stable isotopes, andmetabolism. areas: expenditure energy in two core provides services facility Mass Spectrometry The of stable isotopes. of stable andresources theadministration for andanalysis expertise MISSION PENNINGTON RESEARCH CENTER BIOMEDICAL : The mission The ofthis core is to scientific provide Ph.D.; Valery Hymel, B.S. Hymel, Valery Ph.D.; Xu, Feng B.S.; McCown, Paige M.S.; Broussard, Evest Toth,Bruce M.A.; Staff: DABCC, Ph.D., FACB Rood, C. Jennifer Director: 16 Oxygen and Oxygen 1 Hydrogen, Hydrogen, ------CORE SERVICES 85 - 010–2011 2

Faculty: Alok Gupta, M.D. SCIENTIFIC REPORT SCIENTIFIC Staff: Mandy Shipp, R.D. (Director, Outpatient Ronald Monce, Clinic); P.A.-C.;Patricea Angelle, R.Ph.; Jennifer Arceneaux, R.N.; Brooke Bayham, B.S.; Karen L.D.N., Boley, R.D.; Mary Beth Burnett; Lauren .R.N.; Cox, Leslie B.S.;Currier, L.P.N.; Mavis Crow, Amber Dragg, L.D.N., R.D.; Angela Eldredge, B.S.; B.S.; Janet Fahr, Bethany Gildersleeve, L.D.N., R.D.; Linda Guy; Debbie Hamilton, R.N.; Lauren Harrington, R.N.; Claire Hazlett, R.Ph.; Frances Hutchinson; Jana Ihrig, R.N.; Carolyn Johnson, Pamela L.P.N.; Jolivette, B.A.; Stacie La Prarie, R.N.; Melissa Lingle, B.S.; Ann Liu, Ph.D.; Monica Lockett, Susan Mancuso, L.P.N.; R.N.; Kimberly Marcell; Jennifer Perrault; Kimberly Phillips, B.S.; Dawn Rachal, M.Ph.; Vimala Rajapho, L.D.N, R.D.; Candida Rebello, R.D., J.D.; Lura Reed; Charles Sides, R.Ph.; Marisa Smith, Sonnier, B.S.; Tance B.S.; Allison R.N.; Strate, Jolie Thibodaux, B.S.; Aubrey Windham, B.S.; M.S. Ying Aimee L.P.N.; Yu, Yoches, Director: Frank Greenway, M.D. Adjunct Faculty: Drake Bellanger, M.D.; Thomas Guillot, M.D.; Mark Hausmann, M.D.; Karl LeBlanc, M.D.; Liu, Zhijun Ph.D.; John McClelland, M.D.; John Paige, M.D.; William Raum, Ph.D., M.D.; Brooke Whisenhunt, Ph.D.; Marney White, M.D. Woltering, Eugene Ph.D.; foundations. The Outpatient Clinic participates in multicenter participates Thefoundations. Outpatient Clinic multicenter in products. new industry develop with to collaborates and trials Most of the studies performed Biomedical relate Pennington at obesityto diabetes, including or its complications, associated abnormal fat metabolism, blood high and atheroscle pressure, new research developing now are We disease. vascular rotic programs and sleep aging in abnormalities. ------Alok Gupta, M.D. Associate Professor - PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON MISSION supports: The Clinic Outpatient by trials clinical ing involves visits in the clinic for those who pass the initial for those who visits pass the initial the clinic in ing involves Core. Recruitment the by screening telephone screening volunteers and collecting research data. Screen Frank Greenway, M.D. Greenway, Frank Professor Outpatient Clinic Outpatient eral government, industry, and The Outpatient Clinic employs employs The Outpatient Clinic 44 two people: director, a clinic aphysicians, assis physician 13,032 there were In 2010, 2,483 screenings, telephone 1,790 and visits, screening subjects randomized clinical into new clinical There 39 trials. were with fundingtrials from the fed tory space. and office core nursetant, eleven coordinators, studyeleven five coordinators, dietitians, a project coordinator, a medical with librarian records two assistants, four secretarial personnel, entry data a supervi and a postdoctoral fellow, sor, three part-time pharmacists. ergy X-ray absorptiometry (DXA), nuclear magnetic resonance resonance magnetic absorptiometry nuclear ergy X-ray (DXA), and ultrasound rooms. There optical spectroscopy, is (NMR), for strength testing Core and fitness, Exercise an Testing also locker facilities.including Thelabora second flooreating an has The Outpatient Clinic is on the first is Theand Outpatient Clinic second floors of thenew Biomedical Building, Research Pennington Clinical four-floor occupies 90,000which exami Therefeet 18 square are of space. on the firstThe also Imaging/MRS is en floordual Core has and nation rooms, 2 electrocardiogram/procedurenation rooms, 3 private phlebotomy laboratory 6 with phlebotomy a rooms, interview and a locked chairs, and weight, a room height for measuring pharmacy storage room. There a medical is library records with three space, office physician and three administrative offices, recruiting offices, and reception/waiting areas. 86 CORE SERVICES Proteomics andMetabolomics new experimental methods. to scientists with develop techniques andworks analytical provides a significantamount ofstaff consulting regarding core The applications. andmetabolomics proteomics geted andtar global both for support to bioinformatics provide packages core ofsoftware tions. also The maintains avariety liquid chromatography andseveral worksta robotic systems, seven five with mass spectrometers, is equipped facility The diseases. of prognosis diagnosis for used that and be can molecules and to identify mechanisms by which diseases treatments for are effective, the ofdiseases, thecauses to understand toused understand disease.measurements are These aparticular for important that have molecules to found be biological been more or investigation detailed more for ofone used tools also be can disease. These aparticular for whichlearn are important to in order in asingle experiment ofmolecules thousands tools measurement enable These ofchanges in molecules. to identify, quantify, biological detection andcharacterize chromatography mass with inspectrometric conjunction such as multidimensional technologies separation ful liquid utilizes power Core facility andMetabolomics Proteomics urine,molecules) tissues,andcell The from cultures. blood, proteins, (peptides, lipids, andsmall molecules of biological specializesin theanalysis facility ofdiseases.The the causes to tools investigate andtechnologies array ofcutting-edge attors Pennington an with andaround theworld Biomedical Core investiga provides andMetabolomics Proteomics The Assistant Professor Assistant Indu Ph.D. Kheterpal, biological studies. studies. biological and biomedical for andmetabolomics proteomics to apply andresources to researchers enable and technical expertise MISSION SCIENTIFIC REPORT : The mission The ofthis core is to scientific provide

Ginger Ku, M.S.; Jacob Myers, M.S. Myers, Jacob Ku, M.S.; Ginger Staff: Indu Kheterpal, Ph.D. Faculty: 2 010–2011 - - - - Recruiting Director M.B.A. Rodarte, Ruben phone screens and over scheduled screening visits. 2,600 In 2010 and2011, over Core 21,000 conducted theRecruiting which for they originally called. the study clinical trials in theevent that thecriteria for they not do meet matching to alternative them seamlessly while participants which system, allowsscreen to recruiters potential screen phone to enhance is process theelectronic therecruiting ofthetools developed One follow-up. andtrack to expedite developed application, message-tracking into an electronic istem. that Informationsubsequently inputted from system to avoicemail are directed sys of available callers recruiters, thevolume when ofcalls thenumber exceeds periods During evenly available them across recruiters. calls distributing and by processing large system volumestional ofphone phone ofatradi thecapability expands AUCD system recruiters. call centerenabled by andis ateam operated ofsixfull-time (UCD)- Call aUniform Distributor with core isThe equipped marketfacilitate testing. and efficacy to measure metrics thenecessary provides system tracking cluding online An and advertisement- email advertisements. Meanwhile, novel more have methods employed, in been mailing. newspaper, direct and include television, utilized accrual Traditional process. that mediums are advertisement available resources, and developing tools to streamline the increasing advertisement, of innovative methods establishing goal Our is to accelerate of recruiting. by recruitment ficiency advancement has ef takenand place to improve theefficacy Sincethe public. ofthecore theformalization in 2007, much Core to is Recruiting clinical dedicated promoting The trials to rates. clinical for trials to accelerate accrualservices participant MISSION PENNINGTON RESEARCH CENTER BIOMEDICAL : The goal ofthis goal The core is to recruitment provide

Peters, B.S.; Maria Sanchez, B.S. Sanchez, Maria B.S.; Peters, JamesB.S.; Edmonds, Latrica B.S.; Beech, Jessica B.S.; Bella, Grace B.S.; Dahmer, Brenda M.P.A.; Parks, Ami Staff: M.S. M.B.A., Rodarte, Ruben Director: - - - - CORE SERVICES 87 - - - - 010–2011 2

strive to provide Director: Randall Mynatt ,Ph.D. Staff: Jingying Zhang, Ph.D.; Estrellita Bermudez, M.NS.; Bond, Steven B.S.; Dieyun Ding, B.S. SCIENTIFIC REPORT SCIENTIFIC The mission of this core is to provide controlled provide controlled to is core of this The mission : MISSION manipulation of gene investiga expression facilitate and to manipulation function. understanding gene in tors Microinjection of mutant mouse embryonic stem cells into mouse blastocysts. mouse into cells stem embryonic mouse mutant of Microinjection The Transgenic Core currently produces genetically currently engi Core The Transgenic neered as for faculty Biomedical, well mice as Pennington at pronu institutions. The utilizes other at core investigators microinjectionclear and embryonic technologies stem cell We gene expression control mice. in to services available. commercially are prices below those at that a is cores mostother over offer we that The feature unique service turnkey well for a price generating knockout at mice a full-time cost. By having person below commercial devoted and construction design the we to of the targeting vector, efficient, an money-saving created service Penning have for Biomedicalton investigators. Biomedical Association an has for Assessment Pennington of Laboratoryand Accreditation Care International- Animal approved facility animal capable of housing and providing necessarycare for the number of mice work. for transgenic The barrier portion facility of the animal 3,000 has square feet capable use for transgenic 6,000 of housing There mice. a 400-square-footis the barrier within lab for wet embryo manipulation. Randall Mynatt, Ph.D. Assistant Professor Transgenics - - - Marlene Afton, B.S.; Kelly Director: CourtneyBrock, R.D., L.D.N. Staff: Atteberry, R.D., L.D.N.;Ellen Broussard; Gina Castelluccio, B.S., Lorraine Eames, Betty L.P.N., Fisher; Annette Hutchinson; Renee Puyau, R.D., L.D.N.; Rachel Romaine, B.S.; B.S.; Dawn Turner, Ashley Williams, B.S. This mission of this core is to support to is core nutrition of this mission This : PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON MISSION al research as an integrated component of the Pennington of the Pennington component integrated an as research al preparing, designing, and by Biomedical Center Research serving meet to study-specific meals and criteria produce results. scientific valid Courtney Brock, R.D., L.D.N. Director Research Kitchen Research The Research Kitchen Core is located is onThe the second Kitchen Core floor Research of state-of-the-art a Building. It is Research the Clinical facility equipped is provide that quality to service for ideal and is conducting simultaneous study protocols. Approximately can be per The prepared day core’s meals 225 the facility. in program precisely to plan analysis a nutritional use dietitians and recipesmenus meet to the requirements study of each the clinic into integrated The Kitchenprotocol. is Research Biomedical, and itsscheduling system dieti Pennington at The a director employs core who oversees menu planning, food production, of the operation, management and daily while research dietitians are responsible for managing the dietaryspecific of component studyResearch protocols. specialists and a food service prepare and serve worker the research-designated diets. Meal monitors with partici sit cians workcians directly with principal investigators as they plan study and design Theresearch Kitchen protocols. Research works and Ingestive Behavior Clinic closely with Inpatient the collecting these entities, to providing meals Laboratory, food and waste, food and plate weighing provision by data intake Database. Central Center’s the these into data entering participants that ensure pants being times are to meal during a users by The oversight Kitchen receives Research compliant. committee. ADMINISTRATION & FINANCE

88 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER The Pennington Biomedical Research Center has maintained maceutical companies, the food industry, non-profit health its capability of expanding and growing its scientific, clinical, organizations such as the American Diabetes Association, the and population science research efforts over the past several American Heart Association and the American Cancer Soci- years of economic recession. Now in the third year of a five ety, and various other businesses and not-for-profit entities. year $71 million capital construction and renovation cam- Current researchers have been increasingly successful in paign, a new 90,000 sq ft Clinical Research Center has recently obtaining industry awards and the level of private grants and opened and a state of the art 30,000 sq ft Biomedical Imaging contracts recently exceeded Center is soon to be completed. In all, over 320,000 sq ft of $10 million annually.

new and renovated space position Pennington Biomedical to ADMINISTRATION & FINANCE State Appropriations remain current and competitive in the research arena both nationally and internationally. Unrestricted state dollars are used to fund pilot studies Funding for the center has remained relatively stable despite that result in new grants and shrinking state appropriations due to the variety of funding contracts, as seed money to sources utilized - federal grants, private grants and contracts, recruit new research faculty and state research grants. Additionally, the Pennington and to build new research Biomedical Research Foundation and the Pennington Medical programs at the Pennington Foundation provide funding for periodic capital projects and Mark Alise, Ph.D., M.B.A. Biomedical. The state of Associate Executive ongoing operations. With such diversified funding sources, Louisiana receives a return on Director for Pennington Biomedical has been able to maintain a high level Administration & Finance this investment through an of productivity and research activity in the face of the difficult inflow of research dollars from economic environment. sources outside of the state Federal Grants while also creating new jobs and new wealth in Louisiana. Pennington Biomedical is a continuing success story in at- In the last fiscal year, Pen- taining federal research funding and has consistently been in nington Biomedical received the $20 million range for federal awards over the last three approximately $14 million in years. A large portion of this federal funding comes from the unrestricted state funding, down from a peak of $17 million National Institutes of Health (NIH) grants through a highly three years ago. competitive review process, and the Pennington Biomedical’s research scientists are extremely successful in competing with Outlook for the Future top researchers across the country. Shrinking state appropriations have challenged the Pen- The United States Department of Defense also continues its nington Biomedical Research Center to creatively expand its long-standing research relationship with the Pennington horizons to leverage the unique human and physical resources Biomedical Research Center. In past years, this research has at its disposal. With state of the art facilities and world class delved into a number of facets of military nutrition and fit- research faculty, the major goal and focus will be to generate ness. This relationship continues to be very important to the new partnerships, programs and revenue streams that will en- growth and success of the center with the recent award of a $6 able Pennington Biomedical to remain financially secure and million dollar grant to research optimal war fighter nutrition. successful in realizing its vision for the future.

Private Research Grants and Contracts

Pennington Biomedical has long been recognized by private industry as a premier clinical and basic research institute, resulting in private grants and contracts established with phar-

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 89 90 ADMINISTRATION & FINANCE Computing Services opportunity to in join theCenter’s ofexcellence. pursuit opportunity by andgladly welcome ourinstitution science produced the training ofourstaff. be with proud We associated theare to possible, by thetechnical competent ing themost support We needs. also these place much emphasismeet ensur on to solutions technological and design timely andimplement ofourresearchers, needs theinformation technology identify the Center’s We work continuously research activities. to ment, we to are able improveand enhance overall efficiency administrativefor staff.By enriching the environcomputing theburden anddecrease ourfaculty for orative opportunities intothem offeringsorder in to increase ourtechnology collab to integratevances andplaces ahigh value opportunities on ad ofnewtechnological keeps abreast Computing Services Education, andInfrastructure. Computing, Nutritional Computing, Technical and Support groups: Administrative fourfunctional through its support research andbusiness at operations theCenter. Itprovides ofthe facet every is focus supporting on Computing Services’ Director Guy LaVergne, CCNP B.S., Singh, B.S., Timothy Stafford, B.A., Ying Ying Wu, Ph.D. B.A., Stafford, Timothy B.S., Singh, Sanjana B.S., Parfait, Jennifer B.S., Nguyen, Tim M.C.S., Moss, Vyaisha B.S., LeBlanc, Eric B.S., Landry, Justin Kelly,John M.C.S., M.S., Holmes, Diana B.S., Hannaman, Jeff B.A., Clint Duffy, B.S., Davenport, Franklin B.S., Burt, Spencer Buchanan,CNE, Barry B.S., Brakel, Jason B.S., Acosta, Stephen M.S., Acharya, Turner,B.S., Archana Daniel B.S., Russell, Andrew B.S., Lassalle, Claire Ph.D., Ray Allen, M.B.A., Gravois, Cherie Staff: CCNP LaVergne,Guy B.S., Director: customized application development.customized application collaborative technologies, tools and edge in cutting support FOCUS SCIENTIFIC REPORT Computing Services provides exceptional provides technical : Computing Services

2010–2011

- - - - employees, contractors and external visitors. for tags issuing and parking andmonitoring badges ofID basis fourhour theyear throughout the andfor a twenty providingfor areresponsible officers coverage on Security ofproperty. visitors ofemployees, andtheprotection being andwell is thesafety for responsible department security The through this department. arerelocations movescoordinatedalso personnel andoffice ment avalue with of$1,000 furniture for more. or All requests all moveable andtracking equip tagging andfor packages, and is anddelivering shipping, all for receiving, responsible processes all deliveriesing department made to the Center receiv The projects. million Outlay in Capital construction than thedesign ofmore andimplementation $50supervising Presently, are administering personnel and department additions for facility and renovations. activity construction andmonitors design supervision overallprovides project Residence Center; andsecurity. Facilities Management also control; management oftheon-site and receiving; property shipping reception andmailing services; custodial services; grounds maintenance; services; repairs; utility and equipment maintenance building environmentalor control ofthefacility; is responsibilities with charged theinteri for department The Director Blanchard,Jerry NCARB RA, B.Arch., Facilities Management Research CenterResearch campus. maintenance theentire for Pennington services Biomedical FOCUS Quebedeaux, Lionel Smith and Develine West. Develine and Smith Lionel Quebedeaux, Brandon Doiron, JenniferSteven Heckert, Kirby, Karen Keisha Borel, La Bertrand, Scott Department: Security Receiving Department: BarrettMabile Dwayne Lambert, Wesley. Ken and Smith Charles Saurage, worker), Palmer, James Brett (student Miles Katie Marks, Bryan Paul Johnson, Johnson, Cornelius Clinton Jarrett, Jackson, Jerrol Gentile, Andrew Cynthia Findley,Faucheaux, Gardner, Ronald Diane Fentress, Walter Farr, Corkern, Barbara Adam Broussard, Arthur B.A., McDonald, Robin B.S., Lejeune, Darryl A.I.A., RA, ARCH., B. Walter Legett, B.S., Jordan, Laura B.A.S., Hughes, Marilyn Staff: NCARB RA, B.Arch., Blanchard, Jerry Director: PENNINGTON RESEARCH CENTER BIOMEDICAL : Facilities and operation Management provides - - - ADMINISTRATION & FINANCE 91 - - - - 010–2011 2

Sharon Hebert, B.A., Jason Director: Gena Doucet, M.B.A. Staff: Hymel, B.S., Courtney Henson, B.A., Remy Allen, J.D. SCIENTIFIC REPORT SCIENTIFIC Human Resource Management is committed to committed is Management to Resource Human : Gena Doucet, M.B.A. Director FOCUS providing Pennington Biomedical with a qualified, trained providing Pennington workforceworking by a partner as to with management recruit qualified and retaindiverse a highly workforce. Human Resource Management Resource Human petitive benefitcompensation and programsas appropriate components of the department’s key large are activities. The HRM department responsible for personnel overseeing is all benefitand employee programsoffered to faculty and staff vision, dental, health, partas all including of the LSU System, and dismemberment, long term death accidental life, term flexible the offerings, disability insurance term long and care the options, account spending care dependent and spending tuition exemption program, retirement planning options, and program. assistance employee of an provision ensur dedicated also to is Management Resources Human local and federal, with state, all employment ing compliance laws. The department provides and efficient effective support ser recruitment, as employ areas such in management to vices benefits,ment, reporting, immigration, and andretention of facultyreward and staff. and procedures, policies, all implementing and Developing productive positive, a programs promote which and fair work com for facultyenvironment and fair, designing and staff, - - Thomas Blalock, B.S., Director: M.S. Mougeot, Monica Staff: Yvette Brunswick, Joey Cyrus, B.S., Niki Hays, B.S., Yvette LeBlanc, M.B.A., Diane Lowery, John McClanahan, B.S., Jessica Perkins, B.S., Hillary M.S., Polito, Annette Stacy Potter, Sullivan, B.A., Matt Zylicz, M.S. Fiscal Operations provides sound fiscal fiscal sound provides Operations Fiscal

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON FOCUS management of the Center’s financial assets, ensures assets, financial ensures of the Center’s management compliance with Federal and laws regulations, and State enables timely procurement and delivery of goods and services, and oversees of business-related a multitude functions and services. Monica Mougeot, MS Mougeot, Monica Director Fiscal Operations managers provide individualized financial funding and clinical procured of research the management by Biomedical Detailed faculty. of ac Pennington management The services management Operations provided Fiscal by post-award payable, include accounts payroll, purchasing, contracts management, financial audit, budget preparation collec and audit, reimbursement travel monitoring, and counting and reportingcounting requirements of grants and contracts Operationsthemthe Fiscal affords opportunityby focusto on funded of their the science research. tion of university Operations prepares Fiscal also revenues. and financialall reporting accounting Pennington for the federal, and state, all to relative Biomedical Center Research industry funding. Fiscal Operations Fiscal Facilities Management Facilities 92 ADMINISTRATION & FINANCE Intellectual Property (IPLRA) Property Intellectual Director Anne Jarrett, Ph.D., J.D., M.B.A., L.L.M., M.P.H. mittee, and all other compliance components. (IRB), theInstitutionalwith ReviewBoard Com theBiosafety is HIPAAIPLRA for responsible closely Compliance andworks atprograms theCenter. and as theliaison officesand to Biomedical other regulatory as thecompliance also officeforIPLRA functions Pennington in theUnited States business and worldwide. partnerships toand copyrights, andto license develop technologies these newinventionsproperty, anddiscoveries, including patents is to commercialize Pennington Biomedical’s intellectual development economic mission of The IPLRA oftheOffice ance functions for theentire for center.ance functions compli and regulatory legal, research, of commercialization (IPLRA) oversees involving activities economic development, FOCUS SCIENTIFIC REPORT Intellectual Property, Legal and Regulatory Affairs Affairs LegalandRegulatory Property, : Intellectual

2 010–2011 Warren, B.S. Staff: M.B.A., M.P.H. L.L.M., J.D., Ph.D., Jarrett, Anne Director: Betty Rushing, Janice Janice Rushing, Betty - - external funding. funding. external stewardship andfacilitate proper of toOperations support and Fiscal Affairs LegalandRegulatory Property, Intellectual with closely works tions Projects andfoundations. Sponsored through private corpora funded and clinical trial projects governmentstate, as andlocal well agencies, as research for liaisons awarded representativesby with grants for federal, as serves staff Projects Sponsored The awarded projects. administering and information, proposal processing and preparing opportunities, funding locating in assisted also are researchers Pennington Biomedical lations requirements. and award regu and interpretation of sponsor modifications, post ration and negotiation, subrecipient reporting, monitoring, review and approval, budgetdevelopment, contract prepa include proposal Projects by Sponsored provided services The researchers. Biomedical Pennington of competitiveness to enhancethe Projects sored regulations andfederal andstate allows Spon requirements, sponsor of current opportunities, information funding on research andclinicalsponsored trials, as well as provision post award management of andnon-financial Pre-award Director CRA B.A., Ward, Winona Sponsored Projects administration of externally funded projects. funded administration ofexternally in acquisitionthe and staff and assistanceand to faculty FOCUS PENNINGTON RESEARCH CENTER BIOMEDICAL : Sponsored Projects provides information, provides advice, Projects Sponsored Shelley, B.S. Celeste B.A., Johnson, Danielle Staff: CRA Ward,Winona B.A., Director: Katie Atkinson, B.A., B.A., Atkinson, Katie - - - - Sponsored Projects ADMINISTRATION & FINANCE

At Pennington Biomedical Research Center, our new mission is to discover the triggers of chronic diseases through innovative research that improves human health across the lifespan.

PENNINGTON BIOMEDICAL RESEARCH CENTER SCIENTIFIC REPORT | 2010–2011 93 ADJUNCT FACULTY

“In addition to our own researchers, Pennington Biomedical benefits from the talents, abilities and experience of over 85 adjunct faculty. These adjunct faculty members represent more than 30 universities and research institutes from around the State of Louisiana and the world.”

— Steven B. Heymsfield, M.D., Executive Director

94 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER ADJUNCT FACULTY 95 * deceased * 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Institution Institute Research Weis Medicine Veterinary School University of State Louisiana Medicine Tropical Health and School University of Public Tulane Videoscope SurgeryAdvanced and Kidney Diseases, and Digestive Institute of Diabetes National The Institutes of Health National University Tulane of SouthUniversity Carolina Center NeuroMedical The Rouge Clinic Baton The Southern University University Tulane Scripps Research University State Louisiana Hospital Children’s University Tulane University Tulane Rand Corporation - Monroe of Louisiana University LSU Health Sciences Center Ochsner Medical Center, LLC Biomedical, DiLorenzo Health Sciences Center University State Louisiana Institute Health Research Woman’s Center Agricultural University State Louisiana Health Sciences Center University State Louisiana Medicine Veterinary School University of State Louisiana Clinic of Louisiana Caring Center SeniorLake Care Health Sciences Center University State Louisiana Tourism and Recreation DepartmentLouisiana of Culture, PBRC Title Professor Associate Professor Professor Assistant Instructor Professor Professor Professor Professor Instructor Professor Professor Professor Professor Professor Professor Professor Assistant Professor Professor Professor Assistant Professor Assistant Professor Associate Professor Professor Professor Professor Associate Instructor Professor Associate Instructor Instructor PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Adjunct Employee George Argyropoulos, Ph.D. Argyropoulos, George D.V.M. Ph.D., Baker, David Ph.D. Bazzano, Lydia M.D. Bellanger, Eric Drake FRCP MD, H. Bennett, Peter M.D. Gerald Berenson, P.E.D Blair, Steven M.D. Bolter, John F. M.D.* Bombet, Leon Ph.D. Brown, Sandra Ph.D. Bunnell, Bruce Ph.D. Burris, Thomas Ph.D. Caprio, T. John M.D. Stuart Chalew, Ph.D. M.D., Chen, Wei Ronald Clisham, M.D. Paul M.P.H. M.D., Deborah A. Cohen, FACSM Ph.D., Lisa A. Colvin, Dasa, M.D. Vinod Ph.D. M.D., Daniel DiLorenzo, Robert Dubin, M.D. Karen Elkind-Hirsch, Ph.D. Ph.D. Finley, W. John M.D. Foundas, Anne Ph.D. Joseph Francis, M.D. Fraser, Warren Gahan, M.D. W. Patrick M.D. Earl James Garitty, J.D. Julia George, 2010-2011 ADJUNCT FACULTY ADJUNCT 2010-2011 96 ADJUNCT FACULTY 2010-2011 ADJUNCTFACULTY (CONTINUED) Roy Martin, Ph.D.Roy Martin, Ph.D.Pamela Martin, Nathan Markward, Ph.D. Lan ChiLuu, Ph.D. Luftig,Ronald Ph.D. Jennifer Lovejoy, Ph.D. Lopez,Mandi Ph.D., D.V.M. Zhijun Liu, Ph.D. Lilian Levitan, Ph.D. Natalie Leonard, Ph.D. Lefevre,Michael Ph.D. M.LeBlanc,Monique Ph.D. LeBlanc,Karl M.D. Carl J. Lavie, M.D. Carol Lammi-Keefe, Ph.D. Leslie Ph.D. Kozak, Kennedy,Betty Ph.D. Keenan, Ph.D.Michael Anita S.Kablinger, M.D. Glenn Jones, Ph.D. Hu, Ph.D.Houchun Harry Julie Holden,M.D. Heiman,Ph.D.Mark Hausmann,M.D.Mark Ph.D. Hasek, Barbara Thomas Guillot, M.D. Jimmy Guidry, M.D. Gordon, M.D.Stewart David Glancy, M.D. EmployeeAdjunct SCIENTIFIC REPORT

2 010–2011 Professor Assistant Professor Assistant Professor Instructor Professor Professor Associate Professor Professor Instructor Instructor Professor Instructor Professor Professor Professor Professor Instructor Associate Professor Professor Associate Professor Assistant Professor Instructor Associate Professor Associate Professor Instructor Assistant Professor Assistant Professor Professor Professor PBRC Title University ofCalifornia -Davis Behavioral Medicine Blue Cross/Blue ShieldofLouisiana Louisiana State University HealthSciencesCenter Louisiana State University HealthSciencesCenter Alere Wellbeing,Inc. Louisiana State of University School Veterinary Medicine Louisiana State University Agricultural Center National InstitutesofHealth The National InstituteofDiabetes andDigestive Diseases, andKidney LadyoftheLakeOur College Utah State University Southeastern Louisiana University The Surgeons Group ofBaton Rouge and John OschnerHeart Vascular Institute Louisiana State University Polish Academy ofSciences Louisiana Boards School Association Louisiana State University Louisiana State University HealthSciencesCenter Louisiana State University HealthSciencesCenter CaliforniaUniversity ofSouthern Tulane University Health NuMe The Surgeons Group ofBaton Rouge Baton Community Rouge College LadyoftheLakeOur ofHealth&Hospitals Louisiana Department Louisiana State University HealthSciencesCenter Louisiana State University HealthSciencesCenter Institution PENNINGTON RESEARCH CENTER BIOMEDICAL ADJUNCT FACULTY 97 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC Institution University State Louisiana Medicine Veterinary School University of State Louisiana Health Sciences Center University State Louisiana Health Sciences Center University State Louisiana Health Sciences Center University State Louisiana University State Northwestern Medical University School Verona Health Sciences Center University State Louisiana University Rutgers University Rutgers M. Hebert Center Law Paul University State Louisiana Health Sciences Center University State Louisiana University Columbia University Goteborg Institute Research Translational Health Sciences Center University State Louisiana University Tulane University State Louisiana University State Montclair - USDA Center Southern Research Regional Health Sciences Center University State Louisiana Health Sciences Center University State Louisiana GmbH Medistat University State Louisiana University Missouri State University Yale University State Louisiana Health Sciences Center University State Louisiana Center - Agricultural University State Louisiana PBRC Title Professor Associate Professor Assistant Professor Professor Associate Professor Associate Professor Professor Associate Professor Assistant Professor Professor Professor Instructor Professor Assistant Professor Professor Professor Professor Professor Assistant Professor Instructor Professor Associate Professor Instructor Professor Associate Professor Assistant Professor Assistant Professor Professor Professor Assistant PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Adjunct Employee Kenneth Matthews II, Ph.D. Matthews Kenneth D.V.M. McLaughlin, Ph.D., Leslie Nelson, M.D. Steve Ochoa, M.D. Augusto M.D. Paige, T. John Ph.D. Curtis Phifer, M.D. Pietrobelli, Angelo Ph.D. Primeaux, Stefany Ilya Raskin, Ph.D. Ph.D. Ribnicky, David M.P.H. III, J.D. Richards Edward Rodriguez, Ph.D. Paulo M.D. Shen, Wei M.D. Lars Sjostrom, Smith, M.D. Steve Melinda Sothern, Ph.D. Ph.D. Srinivasan, Sathanur K.,Laura Stewart, Ph.D. Diana Thomas, Ph.D. R.D. Ph.D., Tussing-Humphreys, Lisa M.D. Gabriel Uwaifo, Ph.D. Julia Volaufova, Michaela Vossen, Ph.D. Michael Welsh, Ph.D. Whisenhut, Brooke Ph.D. Marney White, Ph.D. Donald Williamson, Ph.D. M.D. Woltering, Eugene Ph.D. Jolene Zheng, 2010-2011 ADJUNCT FACULTY (CONTINUED) FACULTY ADJUNCT 2010-2011 PUBLICATIONS

98 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER PUBLICATIONS 99 Curr Nutr Am J 2010; 2010; Prog Mol Mol Prog 2010; 010–2011 2 2010; 107(8):3693-7.

Am J Clin Nutr 2010; | 2010; 77(5):407-17.

J Health Poor Care 2010; 32(4):497-507. 2010; 13(6):319-24. 2010; 125(5):900-5. J Clin Endocrinol Metab Prog Mol Biol Transl Sci MolProg Biol Transl Antioxid Redox Signal Redox Antioxid Mt Sinai J Med Age (Dordr) Pediatrics 2010; 49(1):22-30. 2010; Proc Natl AcadU S A Sci SCIENTIFIC REPORT SCIENTIFIC Popul Health Manag Health Popul 2010; 6(12):657-8. Free Radic Biol Med Biol Radic Free 2010; 94:1-8. 2010; 21(4):1184-93. 2010; 21(1):51-7. 2010; 22(5):675-9. 2010; 2010; 59(2):323-9. 2010; 62(4):484-94. Byerley LO, Leamy L, Tam SW, Chou CW SW, and Ravussin E. Development L, Leamy Byerley Tam of LO, a serum profile for healthy aging. 12(12):1371-82. Bruce-Keller AJ, White Ingram CL, Gupta DK, S, Knight Pistell AG, PJ, Morrison and CD Keller JN. activity NOX in brain aging: exacerbation by high fat diet. A recurringButler problem AA with and Kozak the LP. analysis of energy expenditure in genetic models expressing lean and obese phenotypes. Diabetes Butler MK, Kaiser M, Johnson Besse J, J and Horswell R. Diabetes mellitus disease management in a safety net hospital system: translating evidence into practice. LeeByerley SH, Redmann LO, S, Culberson C, Clemens M and Lively MO. Evidence for a novel serum factor distinct from glycoprotein zinc alpha-2 that promotes body fat loss early in the development of cachexia. Cancer Bruce-Keller AJ, Gupta S, Parrino TE, Weidner Knight Ebenezer AG, PJ, AM, H, LeVine 3rd, Keller JN and MarkesberyWR. activity NOX is increased impairment. cognitive mild in Brown DE, Warrington Mautz M, Allen WJ, L,HA, Tefft Gotshalk L and Katzmarzyk Relation between PT. protein levels and C-reactive body sample multiethnic a composition in school of Hawaii. children in Biol Hum CardiovascularBrown and Broussard SC, Geiselman risk in African T. PJ American women attending historically black colleges and universities: the role of dietary patterns and food preferences. Underserved Broyles S, Katzmarzyk Srinivasan Bouchard SR, PT, Chen C, W, Freedman andDS Berenson GS. The pediatric obesityepidemic continues unabated in Bogalusa, Louisiana. 95(4):1634-43. Bouchard C. Definingthe geneticarchitecture the of predispositionto obesity: challenging a but not insurmountable task. 91(1):5-6. Bouchard C. Genetics and genomics of obesity: current status. Boschmann M, Engeli Gorzelniak S, Moro C, Luedtke K, A, Adams F, Rahn G, Mahler A, Dobberstein K, Kruger A, Schmidt S, Spuler S, Luft FC, Smith SR, Schmidt HHand Jordan LMNA J. mutations, skeletal muscle lipid metabolism, and insulin resistance. Sci Biol Transl Bouchard C. Genes and obesity. Preface. 94:xiii. ClevelandBranca ZI, Fubara B, CS, Kumar RT, Maronpot RR, Leuschner C, Warren WS and Driehuys B. Molecular MRI for sensitive and specific detection of lung metastases. Bray GA. Soft drink consumption and obesity: it all about is fructose. Lipidol Opin Bray GA. Obesity: maintenance of weight loss: setting our goals higher. Endocrinol Rev Nat Bray GA. Medical therapy for obesity. Brain Brain Eur J Clin 2010; 2010; Diabetes 2010; 7:38. Appetite Surg ObesSurg Relat Dis 2010; 42(12):2189-96. 2010; 2010; 88(1):191-201. Int J Technol Assess Health Int J Technol 2010; 34(5):919-35. 2010; 1317:100-7. J Int Soc Sports Nutr J Neurosci Res Brain Res Brain Int J Obes (Lond) Med Sports Sci Exerc 2010; 103(10):1433-41. 2010; 91(1):289S-92S. Am J Clin Nutr 2010; 2010; 27(4):250-5. Br J Nutr Adv Ther Adv 2010; 1345:146-55.

2010; 26(1):62-70. 2010; 2010; 64(9):917-23.

2010; 1355:70-85. PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Care Albu JB, Heilbronn LK, Kelley DE, Smith SR, Azuma K,Berk ES, Pi-Sunyer FX and Ravussin E. Metabolic changes following diet and a 1-year exercise intervention in patients with type 2 diabetes.

Adarkwah and Gandjour CC A. Cost-effectiveness of angiotensin- converting enzyme blockers II receptor inhibitors in angiotensin and newly diagnosed type 2 diabetes in Germany. JOURNAL ARTICLES 2010 ARTICLES JOURNAL Anton SD, MartinAnton SD, CK, Han H, Coulon S, Cefalu Geiselman WT, P and Williamson DA. Effects of stevia, aspartame, andsucrose on food intake, postprandial and levels. satiety, insulin and glucose Beavers KM, FC, Hsu Isom S, Kritchevsky Goodpaster SB, Church T, B, Pahor M and Nicklas BJ. Long-term physical activity and inflammatory biomarkers in older adults. Andersen NE, Karl JP, CableAndersen SJ, Williams Rood KW, AJ, NE, JC, Karl Young JP, VitaminLieberman D status HR and in female McClung military JP. training. combat personnel during Res 59(3):627-33. Amici Cuccioloni M, M, Cecarini Angeletti V, M, Barocci S, Rossi G, Fioretti E, Keller JN and Eleuteri AM. Interplay between proteasomes 20S and prion proteins in scrapie disease. PurperaBabic MN, Banfield T, BerthoudBW, HR and Morrison CD. Innervation neurons. receptor-containing leptin skeletal of muscle by Res Brain Barnes MJ, Argyropoulos G and Bray GA. Preference for a high fat diet, but not hyperphagia following activation of mu opioid receptors is blocked in AgRP knockout mice. Barnes MJ, Rogers Meter RC, Van MJ and Hermann GE. Co-localization of TRHR1 and LepRb receptors on neurons in the hindbrain of the rat. Bellanger DE, Hargroder and Greenway AG FL. Mesenteric venous afterthrombosis laparoscopic gastrectomy. sleeve 2010; 6(1):109-11. Antikainen R,Bidel Pukkala S, Hu G, E, Jousilahti Hakulinen P, T and Coffee J. consumption andTuomilehto colorectalrisk of cancer. Nutr Blackburn GL, Wollner S and HeymsfieldSB. Lifestyle interventions for the treatment of class III obesity: a primary target for nutrition medicine obesity the epidemic. in Booth A, Amen RJ, Scott M and Greenway FL. Oral dose-ranging developmental toxicity study of an herbal supplement (NT) and gallic acid in rats. 55(1):37-43. Arguin H, Sanchez M, Bray GA, JC, Lovejoy Peters JC, Jandacek RJ, A.Chaput JP Impact and Tremblay of adopting a vegan diet or an olestra supplementation organochlorine results on plasma from concentrations: two pilot studies. Aronne LJ, S, Moreno Tonstad M, Gantz I, Erondu N, Suryawanshi S, Molony C, Sieberts S, Nayee Meehan J, Heymsfield Shapiro AG, D, SB, Kaufman KD and Amatruda JM. A clinical trial assessing the safety and efficacyinverse agonist, ofCB1R taranabant, in obese a overweight and patients: a high-dose study. 100

PUBLICATIONS 2010 Journal Articles endothelial co-cultures. endothelial co-cultures. adipocyte/ of three-dimensional function hyperinsulinemia on lipolytic of Effects DL. Kaplan Gand Vunjak-Novakovic JM, Gimble JH, Choi regeneration. tissue soft for engineering tissue Adipose DL. Kaplan Gand Novakovic JP, KG,Rubin Marra K, Lee Yoo JM, Gimble JJ, Vunjak- JH, Choi 16(6):1048-54. programme. management an evidence-based in diabetics 2 type among visits of ED use inappropriate influencing Factors R. Horswell Rand Culbertson L, Myers C, Campbell SJ, Chiou 2010; 121(22):2398-406. States adults. United among study aprospective pressure: blood reduced with is associated beverages sugar-sweetened of consumption Reducing LJ. Appel and PJ, CA Anderson JD, Ard BC, Batch Elmer CM, Champagne PH, Lin C, Loria DC, Mitchell B, Caballero L, Chen two’. ‘big the beyond looking of importance the obesity: and overweight adult for factors Risk JP, Despres JP, A, Chaput Tremblay Cand A. Bouchard Astrup AM, Sjodin Biol Chem by CRM1. export nuclear of modulation A-dependent kinase protein by NT-PGC-1alpha of Regulation function and localization subcellular P, TW. Huypens JS, Chang Gettys Y, Aand Zhang Kralli C, Black Metabolism mellitus. 2diabetes type with subjects in supplementation chromium to response physiologic and metabolic the of Characterization B. Wang ZQ Newcomer CK, and Martin JM, Martin XH, Zhang RA, P, J, Pinsonat WT, Rood Cefalu Anderson L, O, Levitan J, Sereda Qin applications. clinical hormones: incretin of role physiologic WT. The Cefalu medicine. of art A1C the WT. TranslatingCefalu practice: “Nondiabetic” to clinical levels adults. young in development risk artery aerobic fitness on the development of incident hypertension: coronary and activity physical of associations Joint S. Jr., Sidney Band Sternfeld PJ, Schreiner CE, Lewis TS, DR, Church Jacobs NS, Evans MR, Carnethon with and without metabolic syndrome. women and men in activity and/or physical diet low-fat from protein C-reactive in PM YoungChanges and Ridker ML, DR. Stefanick SM, Camhi trial. controlled randomized and/or exercise diet alow-fat from fat body in changes and syndrome YoungMetabolic and PT DR. Katzmarzyk ML, Stefanick SM, Camhi risk from childhood. metabolic index-specific mass body adult Predicting GS. Berenson and W, C Chen SR, Bouchard PT, Srinivasan S, Broyles Katzmarzyk SM, Camhi 9. to adulthood. childhood from clustering factor risk PT. Tracking cardiometabolic of Katzmarzyk SM and Camhi Reprod Biol trial. controlled arandomized ewes: in secretion gonadotropin and function ovarian on conjugates peptide fragment-lytic betaCG of dose lowtherapeutic of a effects The PM. Bartlewski Wand Hansel EC, Callery ExercSports men. in mortality all-cause and factors health positive of Effect SP, Hooker X, Sui W, JC, Byun SN. Sieverdes Blair CD, and Lee TS Church SCIENTIFIC REPORT 2010; 285(23):18039-50. 2010; 2010; 10(3):195-213. 2010; 2010; 59(5):755-62. 2010; 42(9):1632-8. 2010; 2010; Assoc Osteopath J Am 110(3 2):S8-S14. Suppl 2010; BRev Eng Part Tissue 16(4):413-26. Diabetes Metab Syndr Relat Disord Syndr Relat Metab Obes Facts Tissue Eng Part CMethods Eng Part Tissue 2010; 59(8):1868-9. 2010; Obesity (SilverObesity Spring)

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2 010–2011 Metabolism Int J Pediatr Obes JPediatr Int Hypertension 2010; 8(2):165-72. 2010; J Eval Pract Clin 2010; 16(5):1157-65. 2010; 2010; 18(3):548-54. 2010; 59(1):54-61. 2010; 2010; 56(1):49-55. 2010; 5(2):122- Circulation 2010; 2010; Med Sci Med J

Diabetes (ACT NOW) study. NOW) (ACT Diabetes of Prevention for Now Actos the in at baseline tolerance glucose impaired in tolerance FD TripathyStentz and glucose of D. Determinants Reaven P, R, Ratner N, Musi S, Mudaliar AE, DC, Schwenke Kitabchi RR, TA, Henry S, Buchanan Clement GA, Bray MA, Banerji RA, DeFronzo 18(2):414-6. in healthy, individuals. activity overweight, system nervous autonomic on restriction caloric 6-month of Impact CT. Pennington Eand Ravussin CK, Martin EA, Moreira L, Jonge de Res Educ Health 2diabetes. type with individuals in behaviour sedentary and activity group intervention on pedometer-based physical cognitive-behavioural A I. Tudor-Locke B, Bourdeaudhuij De Cand Deforche K, Greef De 49(8):1290-7. 2010; proteins. synthesized newly of insolubility and stress proteasome inhibitor oxidative for treatment:implications after toxicity to acute neurons of vulnerability Selective JN. Keller Aand Blasio Di E, Gavilan RM, SO, Uranga PJ, Ebenezer Fernandez-Kim L, K, Zhang Dasuri 48(10):1330-7. response to aging and Alzheimer disease. disease. Alzheimer and to aging response in hydrophobicity protein Increased JN. Keller and WR Markesbery AJ, P, SO, Bruce-Keller Ebenezer K, Dasuri Fernandez-Kim L, Zhang myotubes. primary human in uptake glucose and capacity (AMPK)gamma(3) protein kinase activated oxidative in increased results AMP- encoding gene the of mutation R225W occurring Naturally ME. Harper Rand McPherson RS, Beanlands R, Dent D, M, Kendall Lafontaine JN, DaSilva RA, deKemp SC, Thomas C, Aguer SR, Costford SA, Crawford Youth among Levels study. Activity Physical Canadian the youth: for collection data pedometer of treatment and Process C. Cameron Sand Tudor-Locke CL, Cragg Craig C, 42(9):1639-43. 2010; CANPLAY. Exerc Sci Sports Med epidemiology of youth pedometer-determined physical activity: Descriptive and JM C. Griffiths Tudor-Locke C, Cameron CL, Craig 298(1):E117-26. humans. in by exercise is induced NAMPT muscle Skeletal SR. Smith and KE Conley SA, Jubrias TS, Church H, Xie SC, Thomas DC, Albarado M, Pasarica S, Bajpeyi SR, Costford 34(6):522-32. 2010; Study. Mind Wise the children: for program prevention drug tobacco, and alcohol, Aschool-based SM. Patterson Mand Kulesza CJ, Rash MS, Businelle DE, Kendzor DA, Williamson AL, Copeland PARL. protease rhomboid mitochondrial the and insulin signaling by muscleoxidative of skeletal Regulation capacity MW, E. Hulver Ravussin SR and Smith GR, W, Collier Cefalu K, Walder L, Mandarino CB, Newgard N, J, Lefort Finlayson C, TC, Moro Becker A, RP, McMillan L, Kerr-Bayles S, Pierce Carling PS, MacLean AE, Civitarese 42(4):708-16. study. C-reactive INFLAME the protein: reduce not does loss weight without T, Exercise SN. Blair Rand Ross Saunders RQ, CP, Rodarte EL, Priest Earnest TS, AM, Church Thompson JAMA trial. controlled arandomized 2diabetes: type with patients in levels hemoglobin on A1ctraining CP. resistance and aerobic Earnest of Effects Aand Thompson L, Sparks RQ, V, Rodarte Myers M, CR, Nauta Mikus K, W, Johnson Kramer N, Johannsen S, Cocreham SN, Blair TS, Church 2010; 53(9):1986-97. 2010; 2010; 304(20):2253-62. PENNINGTON RESEARCH CENTER BIOMEDICAL 2010; 25(5):724-36. 2010; 2010; 42(3):430-5. 2010; Exerc Sci Sports Med Diabetologia Am J Physiol Endocrinol Metab JPhysiol Endocrinol Am 2010; 2010; Exerc Sci Sports Med 2010; 53(3):435-45. 2010; Free Radic Biol Med Obesity (SilverObesity Spring) Cell Metab Free Radic Biol Med 2010; 11(5):412-26. Res Ther Cognit Diabetologia 2010; 2010; 2010; 2010; 2010; 2010;

PUBLICATIONS 101 Asia 2010; 2010; 2010; 2010; 010–2011 2010; 477(3):129- 2

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2010; 18(10):2047-54. Neurosci Lett Neurosci J Med Internet Res Internet Med J 2010; 103(1):38-42. J Clin Endocrinol Metab 2010; 104(7):1074-9. 2010; 30(4):E57-63. Obesity (Silver Spring) Obesity (Silver 2010; 34(5):886-91. 2010; J Diabetes 2010; Technol Sci 4(3):723-32. Br J Nutr Br J Nutr Obesity (Silver Spring) Obesity (Silver SCIENTIFIC REPORT SCIENTIFIC Med Decis Making Decis Med 2010; 18(8):1646-51. 2010; 19(1):31-42. 2010; Int J Obes (Lond) Am J Clin Nutr 2010; 92(5):1089-93. Galgani JE, Greenway FL, Caglayan S, ML, Wong Licinio J and Ravussin E. loss-induced prevents metabolic weight replacement Leptin adaptation congenital in leptin-deficient patients. 95(2):851-5. Galgani JE and Ravussin E. Effect of dihydrocapsiate on resting metabolic rate in humans. Galgani JE, Ryan DH and Ravussin E. Effect of capsinoids on energy metabolism in human subjects. Galgani JE, de Jonge L, Rood JC, AA Smith SR, and Ravussin Young E. Urinary excretion: C-peptide a novel alternate measure of insulin conditions. physiological sensitivity in BradstreetGallagher Thornton D, W, JC, He TE, Yu Q, J, Burke Wang HeymsfieldJ, SB, RivasVM and Kaufman R. Quantitative magnetic resonance fat measurements in humans correlate with established methods but are biased. A,Gallant AR, Perusse L, Tremblay Bouchard C, Despres JP and Drapeau The Three-FactorV. Eating Questionnaire and BMI in adolescents: results from the Quebec family study. Gandjour population A. preferences Theoretical v. patient foundation of in calculating QALYs. Gandjour A. Investment in quality improvement: maximize how to the return. Health Econ 18(9):1852-7. 37(6):726-37. Freitas RM, Jordan Lipoic J and Feng D. acid effects on monoaminergic system after pilocarpine-induced seizures. 33. Frohlich M, Grayson WL, Marolt Gimble D, JM, N and Kregar-Velikonja G. Bone graftsVunjak-Novakovic engineered fromadipose- human derived stem in cells perfusion bioreactor culture. 16(1):179-89. K,Fujiwara Domichi Tsuzaki S, Matsuoka Kotani K, M, Brantley Y, PJ, Kajii E and SakaneSano N. Y, Dietary salt reduction in rural patients with albuminurea using family and community support: the Mima study. Med Fam 2010;Pac 9(1):6. Appel LJ, KL, ChampagneFunk VJ, Bauck Stevens A, CM, Brantley PJ, Harvey-Berino Jerome GJ, KennedyCoughlin Hollis Dalcin J, J, AT, JF, Myers VH, Samuel-HodgeBM, Lien LF, LP and C, WM. Vollmer Svetkey Associations of Internet Use With Website Change Weight in a Long- term Loss Maintenance Weight Program. 12(3):e29. Gabriele JM,Stewart TM, Sample A, AB, Davis Allen R, Martin CK, Newton and WilliamsonRL, Jr. DA. Development of aninternet-based obesity programprevention for children. Galgani JE, de Jonge L, Most MM, Bray GA and Smith SR. Effect of a 3-day high-fat feeding period on carbohydrate balance and ad libitum energy intake in humans. Fountaine RJ, Taylor AE, Mancuso JP, Greenway SmithFL, Byerley LO, Fountaine AE, Mancuso RJ, JP, Taylor SR, MostMM and Fryburg DA. Increased food intake and energy expenditure following administration of olanzapine healthy to men. Spring) Obesity (Silver Freedman DS, Katzmarzyk Dietz PT, WH, Srinivasan and SR Berenson GS. The relation of BMI and skinfold thicknesses risk to factors among young and middle-aged adults: the Bogalusa Heart Study. Ethn 2010; 2010; Endocrinology 2010; 22(3):839-48. 2010; 2010; 33(3):175-85. Eur J Appl 2010; Physiol J Clin Endocrinol Metab 2010; 108(6):1497-500. 2010; 7:41. 2010; 22(4):521-8. J Alzheimers Dis 2010; 45(6):479-89. J Appl Physiol Fam CommunityFam Health Lipids 2010; 3(5):454-61. 2010; Nutr Metab (Lond) Metab Nutr 2010; 14(4):982-91. 2010; 28(12):1355-9. Curr Opin Immunol Opin Curr 2010; 5(7):1294-311. 2010; 2010; 65(3):284-91.

2010; 20(4):383-9. 2010; PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Dixit Thymic VD. fatness and approaches enhance to thymopoietic fitness in aging. Deiuliis JA, Liu LF, BeluryDeiuliis MA, JA, Liu LF, Rim JS, Shin S and Lee K. Beta(3)- adipose acutely downadrenergic regulates triglyceride signaling lipase brown adipocytes.in 151(6):2547-55. Dubbert PM, Robinson JC, Sung JH, Ainsworth BE, Wyatt SB, Carithers T, Newton Rhudy R, Jr., JL, Barbour Physical K, H, Jr. Sternfeld B and Taylor activity and obesity in African Americans: the Jackson Heart Study. Ebenezer PJ, Weidner AM,Ebenezer LeVine H, 3rd, Markesbery PJ, WR, Murphy MP, Zhang L, Dasuri K, Fernandez-Kim Bruce-Keller SO, AJ, Gavilan E and Keller JN. Neuron specifictoxicity of oligomeric amyloid-beta: role for JUN-kinase and oxidative stress. Do R, Pare G, Bailey Montpetit SD, A, Desbiens S, K, Yusuf Hudson TJ, Bouchard C, Gaudet Perusse L, D, Anand MC, S, Vohl Pastinen T and Engert JC. Fine mapping of the insulin-induced gene 2 identifies a variant associated with LDLcholesterol and total apolipoprotein B levels. Circ Cardiovasc Genet Onur S, Fischer A,Doring Boulay MR, F, Perusse Rauramaa L, Rankinen T, R, Wolfarth B and Bouchard C. A common haplotype and the Pro582Ser polymorphism gene in of the hypoxia-inducible (HIF1A) factor-1alpha elite endurance athletes. Doring Onur FE, S, Geisen Boulay MR, U, Perusse Rauramaa L, Rankinen T, R, Wolfahrt B and Bouchard C. ACTN3 R577X and other polymorphisms are not associated with elite endurance athlete status in the Genathlete study. J Sports Sci Dorsey KB, Thornton JC, HeymsfieldSB and D. GallagherGreater lean and skeletaltissue muscle associated are mass with higher bone mineral in children.content Downey LH, Kennedy Castellanos B, DC, K, Yadrick Threadgill P, Strickland E, Prewitt TE and Bogle M. Capacity building for health community-basedthrough participatory nutrition intervention research ruralin communities. Drel VR, Zhang Lupachyk Delamere J, S, Shevalye W, H, I, Vareniuk Xu NA, Shahidullah M, Slusher B and Obrosova IG. New therapeutic and biomarker discovery for peripheral diabetic inhibitor, neuropathy: PARP nitrotyrosine, tumor and necrosis factor-{alpha}. Dis Blair and SN Church TS. Age attenuatedEarnest response CP, aerobicto women. postmenopausal in conditioning 110(1):75-82. Carnethon MR, Blair and SN Poirier Church TS. P, Autonomic Earnest CP, function and change in insulin for exercising postmenopausal women. Maturitas Elbaz A, X, Wu Rivas Gimble D, JM and Duque G. Inhibition of fatty acid biosynthesis prevents adipocyte lipotoxicity on human osteoblasts in vitro. Mol J Cell Med Esparza-Romero Valencia ME, J, Martinez ME, Ravussin E, Schulz and LO Bennett PH. Differences in insulin resistance in MexicanU.S. and Pima Indians with normal glucose tolerance. 95(11):E358-62. Diekman IsolationEstes Gimble of BO, adipose- BT, JM and Guilak F. derived stem and cells their induction a chondrogenic to phenotype. protocols Nature Journal ArticlesJournal 2010 102

PUBLICATIONS 2010 Journal Articles spermatogenesis. for pad fat epididymal the of W. essentiality Hansel The level over 20 years and weight gain. gain. weight and over 20level years activity physical PJ, ahigh Schreiner Maintaining S. Sidney Kand Liu CE, Lewis M, Carnethon C, Bouchard ML, Daviglus AL, Hankinson antiporter. the cystine-glutamate and oxidase NAPDH of role HIV-Tat release: glutamate microglial elicits AJ. KF, PE, Hauser Bruce-Keller AG, Knapp and JN Knight S, Keller Gupta Res Hypertens profile. risk cardiometabolic adverse an for amarker adults: disease-free in WD. Prehypertension FL Johnson and Greenway M, McGlone AK, Gupta milieu. proinflammatory systemic exacerbated an with correlate adults obese free disease in prehypertension and WD. Prediabetes Johnson AKand Gupta adults. obese in asymptomatic profiles cardiometabolic adverse for markers pragmatic function: endothelial and variability pressure blood in circadian WD.Johnson Abnormalities V, Fand Dhoopati FL, Halberg Greenway G, Cornelissen AK, Gupta 24(5):289-96. changes. weight of independent mellitus Type-2 in fat liver diabetes reduces metformin, not but Pioglitazone, SR. Smith and WD Johnson CK, Martin FL, Greenway GA, Bray AK, Gupta 468(9):2530-40. for musculoskeletal tissue engineering. tissue adipose cells from stem adult Award: Multipotent Andry Nicolas 2010 JM. F,Guilak Gimble and BT, FT BO, Moutos Estes Diekman 24(11):1717-26. of HIV-associated lipoatrophy.indicates persistence participants control and HIV-infected in over 5years by MRI measured tissue adipose P, Regional R. Bacchetti PC, Scherzer Mand Tien S, Shlipak Heymsfield R, J, Jr., Kronmal CE, Lewis Cofrancesco M, Saag C, Grunfeld 12(1):58-63. agents. altering disease and symptomatic as drugs based eserine therapeutics, experimental to Alzheimer products From natural DK. Lahiri Kand JT, Rogers Sambamurti ML, Maccecchini DK, Ingram Yu HW, RE, Holloway Q, Tweedie Becker NH, D, T,Greig W, Luo Utsuki Lancet trial. 3 phase placebo-controlled, a multicentre, double-blind, randomised, (COR-I): adults obese and overweight in loss weight on bupropion plus naltrexone of C-IS. Group Eand DD, Effect J,Dunayevich Kim Erickson M, Guttadauria S, Mudaliar RA, Plodkowski K, Fujioka FL, Greenway Diab Rep obesity. treating for drugs Combination GA. FL Bray and Greenway investigational drugs syndrome. metabolic of treatment the for therapeutics Plant-derived DM. Ribnicky and WT Cefalu I, Raskin BL, Graf Ther cells. tissue-derived adipose using therapies cell-based of translation preclinical and Clinical BA. Bunnell Fand Guilak JM, Gimble incidence. hypertension and depression between relationship the of mediators as duration sleep and Insomnia TG. Pickering and GK Zammit JB, SB, Turner Heymsfield LA, Babiss D, K, Malaspina Posner JE, Gangwisch programs. management disease of cost-effectiveness the to predict Amodel A. Gandjour SCIENTIFIC REPORT 2010; 1(2):19. 2010; 2010; 10(2):108-15. 2010; Am JHypertens Am 2010; 376(9741):595-605. 2010; Endocrinology 2010; 11(10):1107-15. 2010; 2010; 33(9):905-10. 2010; Health Econ Health J Inflamm (Lond) Inflamm J 2010; 23(1):62-9. 2010;

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Neurosci Lett 2 010–2011 JAMA 2010; 19(6):697-715. 2010; Cardiovasc Diabetol Cardiovasc Clin Orthop Relat Res Relat Orthop Clin 2010; 7:36. J Diabetes Complications 2010; 304(23):2603-10. 2010; Current opinion in in Current opinion 2010; 485(3):233-6. 2010; AIDS 2010; 2010; Prog Nutr Prog Stem Cell Res 2010; 9:58. 2010; 2010; 2010; 2010; 2010; Curr Curr 2010; 2010; Heymsfield SB. Meal replacements and energy balance. balance. energy and replacements Meal SB. Heymsfield health. influenceof jointpain, mental and impairment: comorbidity, functional and Obesity and SB MS. Faith Wang D, A, Heymsfield Pietrobelli M, Heo Poor Underserved mortality. and care in disparities gender and race/ethnicity on program management disease astandardized of impact The LM. Arcement Hand Tamariz Li A, R, Palacio L, Horswell B, Lopez K, Hebert J Med South failure. B heart stage screening for peptide B-natriuretic ultrasound, Handheld P, Heidenreich L. R, Arcement Jand Miranda Horswell K, Hebert Metab Guideline. Practice Clinical Society Endocrine an patient: Endocrine and nutritional management of the post-bariatric surgery Still C. Jand Salvador E, Livingston LM, D, Kaplan FL, Greenway Heber 41(2):315-21. 2010; activity. metabolic and locomotor of changes circadian Pan W. Fand IL-15 Halberg B, Robert H, in results deletion receptor Hsuchou GG, Cornelissen-Guillaume AJ, Y,He Kastin RS, Khan Wu X, 30(13):4725-34. memory. hippocampal-dependent transmission and Pan W. X and GABA Guo Z, Interleukin-15 to facilitate is essential receptor PJ, Pistell RS, Khan J, Li Feng Wang AJ, Y, WH, He Kastin Wu H, X, Hsuchou Physiol Regul Integr Comp Physiol states. fasted and fed both in respiration uncoupled increases and TW. methionine Dietary restrictionenhancesGettys metabolic flexibility P, J,Shin Huypens Nand EP, Plaisance VL, Orentreich Malloy RA, Krajcik C, Black NR, Lenard A, Boudreau TM, Henagan LK, Stewart BE, Hasek 2010; 100(1):90-4. 2010; analysis. analysis. population-based regional dialysis--a of LC, Costs Trapp M. Koch Rand W, Rump Rathmann N, G, Giani Chernyak A, Gandjour B, Haastert A, Icks response. insulin tissue systemic andinflammatory resistance adipose exacerbates but ameliorates diet-induced adiposity 6-phosphofructo-2-kinase inducible of Disruption Wu Ye and C. YE, J, RS Chen Chesney J, Chapkin Y,Huo W, Wang Zhang H, H, Li X, Wang Y, Guo Telang Z, Gao H, S, Zhou failure. heart of risk the on ratio waist-to-hip and circumference, waist index, mass body activity, physical of effects Tuomilehto and PT J. Joint P, Jousilahti G, Hu Katzmarzyk R, Antikainen findings. new some for areview disease: Parkinson’s of risk the and Total G. Hu cholesterol barrier. blood-brain across the transport and influencesurocortin development receptor-1hormone in microvessels cerebral during changes Tu Wu X, AJ, Pan W. Hand Kastin H, Corticotropin-releasing Hsuchou 115(5):1288-98. cells. endothelial brain human hCMEC/D3 across onleptin permeation leptin receptor subtypes of astrocytic Role Pan W. and PO Couraud N, Tu Abbott AJ, Joan H, Kastin H, Hsuchou 2diabetes. type with patients for human of inhaled insulin in regimen aTitration (Exubera) treat-to-target J. Rosenstock Dand Lawrence M, PA, WT, Mitnick Cefalu Hollander adults. tall in expenditure energy total mass-specific Reduced requirements: energy human and size Body A. Pietrobelli and SB Heymsfield 2010; 95(11):4823-43. PENNINGTON RESEARCH CENTER BIOMEDICAL 2010; 22(3):301-9. 2010; Biol JHum Am Obesity (SilverObesity Spring) Endocrinology Nephrol Dial Transplant J Biol Chem J Biol 2010; 21(1):264-76. 2010; 2010; 103(7):616-22. Parkinsons Dis Parkinsons 2010; 285(6):3713-21. 2010; 2010; 151(3):1221-7. 2010; 2010; 18(10):2030-8. 2010; 2010; 25(5):1647-52. 2010; 2010; 299(3):R728-39. Diabetes TechnolDiabetes Ther 2010; 2010:836962. Circulation J Neurochem 2010; 121(2):237-44. 2010; J Neurosci J 2010; 12(3):185-91. 2010; J Mol Neurosci Physiol Behav J Clin Endocrinol Endocrinol Clin J J Health Care J Health 2010; 2010; 2010; 2010; Am J Am

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Mayo Clin Proc 2010; 7:7. 2010; 1(3):217-32. 2010; 5(2):e8978. Int J Obes (Lond) Mamm Genome Obesity (Silver Spring) Obesity (Silver Cartilage PLoS One J Int Soc Sports Nutr SCIENTIFIC REPORT SCIENTIFIC 2010; 151(7):3015-25. 2010; 94:75-123. 2010; 2010; 110(2):291-5. 2010; 30(16):5713-23. Med Sports Sci Exerc 2010; 42(1):152-9. 2010; 91(2):366-72. 85(3):239-46. 34(6):1001-10. Leshan RL, Opland Leinninger DM, Louis GM, GW, Patterson CM, Rhodes CJ, Munzberg H and Myers tegmental Ventral MG, area Jr. leptin receptor amphetamine- cocaine-and regulate and to project specifically neurons regulated transcript neurons of the extended central amygdala. Neurosci Levy E, Lastor Y, Wang K and ChampagneLin PH, Miwa S, C. Li YJ, Factors influencing dietaryPREMIER trial the in population. sources protein Assoc Diet Lin X, Racette SB, Lefevre M, Spearie CA, Most M, Ma L and Ostlund TheRE, effects Jr. of phytosterols present in natural food matrices on cholesterol metabolism and LDL-cholesterol: a controlled feeding trial. Eur J Clin Nutr 2010; 64(12):1481-7. Sui X, Church TS, GW, LavieLyerly CJ, Hand GA and Blair SN. Maximal exercise electrocardiographic responses and coronary heart disease syndrome. metabolic mortality with men among Larson-Meyer DE, Redman L, Heilbronn LK, Martin and CK Ravussin E. Caloric restriction with or without exercise: the fitnessversus fatness debate. Lenard NR, Zheng H and Berthoud HR. Chronic suppression of mu- opioid receptor signaling in the nucleus accumbens attenuates obesity rats. in diet-induced of development Larson-Meyer DE, Ravussin E, Heilbronn L and DeJonge L. Ghrelin and peptide YY postpartum in lactating nonlactating and women. Nutr 2010; 34 Suppl 1:S23-7. 2010; Newman S, Chao PM, Mendoza T and Koza RA.KozakLP, The early determines capacity the mice nutritional of environment for adipose tissue expansion modulating by genes of caveolae structure. JL,Kuk Church TS, Blair and SN Ross R. Measurement site and the association between visceral and abdominal subcutaneous adipose tissue with metabolic risk in women. 18(7):1336-40. KG, DiCarloKumar LM, Zuberi J, Volaufova AR and Richards BK. Increased physical activity cosegregates with higher intake of carbohydrate and total calories in a subcongenic mouse strain. Kozak LP and Koza RA.The genetics of brown adipose tissue. Sci Biol Transl Koza RA and Anunciado-KozaKozakLP, R. Brown fat thermogenesis and body weight regulation relevance in mice: humans. to 2):52-63. N,Kumar Rogers Solt LA, PM, Bhattacharyya Y, Wang G, Kamenecka TM, Stayrook KR,Crumbley C, ZE, Gimble Floyd TP. JM, Griffin PR and Burris Regulation of adipogenesis natural by and synthetic REV-ERB ligands. Endocrinology 2010; 5(6):e11015. Kreider L, Campbell RB, Wilborn Taylor B, Almada AL, CD, Collins R, Cooke M, Greenwood M, KalmanEarnest DS, Kerksick CP, CM, Kleiner SM, Leutholtz B, Lopez H, Lowery LM, Mendel R, Smith A, Spano M, Wildman R, Willoughby DS, Ziegenfuss TN and exercise Antonio ISSN J. & sport nutrition review: recommendations. & research Kruger C and Kappen C. Expression of cartilage developmental genes in Hoxc8- and Hoxd4-transgenic mice. Kruger C and Kappen C. Microarray analysis of defective cartilage in Hoxc8- mice. and Hoxd4-Transgenic

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Aging Cell Aging 2010; 11(4):263-7.

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Johannsen DL and Ravussin E. Can increased muscle ROS scavenging keep older animals young and metabolically fit? 18(10):1988-95. Jazwinski SM, Kim S, Li Dai L, J, Bi X, Jiang JC, Arnold Batzer J, MA, Walker DA, LefanteJA, Welsh CM, Myers J, Volaufova L, LJ, Su Hausman DB, Cherry Ravussin KE E, Poon and LW, MA. Welsch Miceli MV, HRAS1 and withLASS1 APOE are associated with human longevity and healthy aging. Johannsen DL, Calabro MA, Rood Stewart JC W, GJ. and Franke J, Welk Accuracy of armband monitors for measuring daily energy expenditure adults. healthy in Inoue S, Ohya Y, Odagiri Y, Takamiya T, KamadaM, Okada T, S, Tudor-Locke Takamiya OdagiriInoue Y, S, Ohya Y, CharacteristicsC and Shimomitsu T. of accelerometry respondents a to mail-based surveillance study. Jackson AW, LeeJackson DC, Sui X, Morrow AW, JR, Church TS, Maslow Jr., AL and Blair SN. Muscular strength inversely is related prevalence to and incidenceof obesity in adult men. 12(6):557-8. Johannsen DL and Ravussin E. Obesity in the elderly: faulty is metabolism blame? to Katzmarzyk Physical activity, PT. sedentary and health: behavior, paradigm paralysis or paradigm shift? Johannsen NM, Blair Priest and SN Church TS. EL, Dixit Earnest VD, CP, Association of white blood cell subfraction concentration with fitness and fatness. Pharm Des Kastin AJ Concepts for and biologically W. Pan active peptides. Katzmarzyk Bray GA, Greenway PT, FL, Johnson Newton WD, RL, Jr., Ravussin E, Ryan DH, Smith and SR Bouchard C. Racial differences in abdominal depot-specific adiposity in white andAfrican American adults. Johnson CA,Kennedy BM, Karanja Kumanyika N, Reams S, Ard P, JD, Charleston JB, Appel LJ, Maurice Overall V and Harsha and minority- DW. focused recruitment strategies in the PREMIER multicenter trial of lifestyle interventions for blood pressure control. Kipnes MS, Hollander P, Fujioka K, Kipnes Lu Gantz Erondu MS, N, Hollander Shentu I, Seck Y, T, P, K, Suryawanshi S, Chou M, Johnson-Levonas Heymsfield AO, SB, Shapiro KaufmanD, KD and Amatruda JM. A one-year study assess to the safety inverseand efficacy agonistCB1R taranabant of overweight the in and obese patients with type 2 diabetes. 2010; 31(1):49-54. Khan C, Kastin RS, Ehrensing RH, Yu AJ, Hsuchou H, He KP Stone Y, and Brain Activation W. Pan Peptide by Pro-Leu-Gly-NH(2) (MIF-1). 2010; 2010 Kheterpal I, Coleman L, G, Ribnicky Ku ZQ, Wang D and Cefalu WT. action extractRegulation insulin an of by Artemisia of dracunculus L. in primary human skeletal muscle culture: a proteomics approach. Phytother Res Metab 12(6):517-31. Kisby GE, Kohama SG, Olivas A, Churchwell M, Doerge Spangler D, E, de Cabo R, Ingram DK, Imhof B, Bao YW. G and Effect Kow of caloric restriction on base-excision repair in the (BER) aging rat brain. Gerontol Brown fat and themyth of diet-inducedKozak LP. thermogenesis. Journal ArticlesJournal 2010 104

PUBLICATIONS 2010 Journal Articles adipogenesis. human and murine of analysis ED.epigenomic Rosen Comparative and ES Lander JM, Gimble Wang L, Xu X, Zhang TS, Z, Mikkelsen Neurochem signaling. Nrf2 decreased for mice: implications aged in impairment oxidative and cognitive stress hippocampal increases diet High fat JN. Keller and AJ Bruce-Keller RM, Uranga MN, Purpera CL, SO, White WD, CD, Y, Liu PJ, Pistell Johnson Morrison DK, Fernandez-Kim Ingram (SilverObesity Spring) exercise. intensity moderate of benefits loss weight the and genotype T, CP, Rankinen Earnest TS, Church JA, FTO Mitchell SN. Blair Xand Sui 160(1):102-8. 2010; J Heart Am mortality. disease cardiovascular on factors health combined of impact The SN. Blair and SP, Hooker X, Sui DB, CD, DC Lee Bornstein Lee JA, TS, Church Mitchell rats. in mortality increases and inducesvacuolization cardiac of 2-deoxy-D-glucose Chronic ingestion JA. Mattison and DK Ingram R, Cabo de DB, Allison M, Lane GS, Roth EL, Spangler S, Poosala S, Kaushik AM, Jr., DL, Sossong Smith RK, Minor 1 diabetes. LY, type of WT. Complications Cefalu and Melendez-Ramirez RJ Richards 50(1):72-87. reflex. light pupillary human the of dynamics response and sensitivity spectral the cells on ganglion retinal photosensitive intrinsically- of PD. influence Gamlin and The DH McDougal Disord Musculoskelet pain. back low chronic for programme walking astructured plus advice and education versus advice and education trial: 2activity Back The DA. Hurley and DG Tudor-Locke I, Baxter C, Bradbury A, Delitto DP, Kerr A, Boyd Tully SR, SM, SM, O’Connor MA, O’Neill McDonough Health accelerometer. ActiGraph the with sensors motion cost 3 low of Tudor-Locke and validity Convergent RS C. Getz TL, JJ, Hart McClain Clin Nutr siteimportant? is measurement risk: cardiometabolic of prediction the for PT. thresholds Waist circumference Katzmarzyk Cand Mason islands. Azores the from children of BMI in changes of Correlates G. Beunen Pand V, Lopes R, Katzmarzyk Garganta A, D, J, Seabra Maia Martins recommendations. ofthe Institute Medicine than energy and fat saturated more containing foods select cafeterias school in Children DA. Williamson and CM Champagne A, Sample H, Jr., RL, Han Newton TM, Stewart MM, LeBlanc JL, Thomson CK, Martin partnership. corporate of effect the and program weight-loss acommercial in retention and O. Weight Khavjou loss and AM Hymel Talamini A, CK, Johnson L, Martin Diabetes Care patients. diabetic in ghrelin and intake energy on pioglitazone of Effect GA. Bray Hand Han FL, Greenway SR, Smith AK, Gupta CK, Martin diabetes: a meta-analysis. a meta-analysis. diabetes: 2 type and syndrome metabolic of risk and beverages sweetened JP, Despres FB. Sugar- Hu WC and GA, Bray BM, Willett Popkin VS, Malik risk. disease cardiovascular and mellitus, 2diabetes type obesity, beverages, FB. Sugar-sweetened Hu and JP Despres GA, Bray BM, Popkin VS, Malik SCIENTIFIC REPORT Circulation 2010; 7(5):662-70. 2010; Int J Obes (Lond) JObes Int 2010; 64(8):862-7. 2010; 2010; 114(6):1581-9. 2010; Endocrinol Metab Clin NorthAm 2010; 33(4):742-4. 2010; Cell 2010; 121(11):1356-64. 2010; 2010; 143(1):156-69. 2010; 2010; 11:163. 2010; 2010; 18(3):641-3. 2010; Int J Obes (Lond) JObes Int 2010; 34(10):1487-93. 2010; Diabetes Care

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2 010–2011 2010; 34(4):742-50. 2010; 33(11):2477-83. 2010; 2010; 39(3):625-40. 2010;J Nutr 140(9):1653-60. 2010; 243(3):332-9. 2010; Vision Res Vision J Phys Act J Phys Act BMC 2010; 2010; Eur J Eur J Pasarica M, Rood J, Ravussin E, Schwarz JM, Smith SR and Redman LM. LM. Redman SR and Smith JM, Schwarz E, J, Ravussin Rood M, Pasarica 25(6):623-30. 2010; receptors. cytokine transporting and transporter inflammation:regulation efflux differential of in LPS-induced NFkappaB vascular cerebral of role The Pan W,AJ. Kastin Yu Hand Hsuchou C, Allergy Clin Immunol adiposity, fitness, andinincident adults. asthma Cardiorespiratory SN. YJ, Cheng TJ, JR, Church Ruiz X, Sui CC Blair Miller DC, and FB, Lee Ortega 177(3):1436-47. 2010; neuropathies. autonomic and peripheral fiber small and large of diabetic in roles Different 12/15-lipoxygenase RE. Schmidt and JL Nadler Vareniuk I, H, Shevalye VR, Drel R, Stavniichuk IG, Obrosova 26(1):135-42. rat in retina. injury on ischemia-reperfusion fidarestat inhibitor reductase aldose CD. the Agardh of AB and Evaluation Y, P, Maksimchyk Pacher IG, Obrosova El-Remessy ML, Smith E, Agardh management. and mechanisms PF. Kador SS and Chung cataracts: IG, Diabetic Obrosova version of the Stone mice. T-maze the of for version motivated awater-escape of Development PJPistell DK. Ingram and mice. C57BL/6 of chronic lopinavir/ritonavir consequences neurologic to administration and Metabolic AJ. Bruce-Keller and JN Keller C, Ruiz I, Kheterpal DK, Ingram RM, Uranga M, PJ,Pistell AG, Domingue Knight S, Gupta 2010; 135(7):1631-5. monomeric (1-40) Abeta peptide by electrophoresis. capillary JP, Moses R, Picou SD. of Gilman Analysis Iand Wellman AD, Kheterpal Phys Act JAging trial. Pilot Elders for Independence and Interventions Lifestyle the in illness of because activity physical of Interruption RA. Fielding Tand Church Walkup MP, ME, WJ, J, Miller Katula AC, Rejeski EM, Phillips King JS, Brach circulating cholesterol. DY regulates and Tschop signaling in the CNS directly Melanocortin MH. MW, RD, Hui Sleeman DiMarchi SC, Benoit SC, Woods WS, Davidson AA, Trevaskis Butler M, JL, Arnold NA, Granholm HE, JT, Wilson-Perez E, Grant PT, Pfluger Patterson R, J, Nogueiras Basford SM, Perez-Tilve D, Hofmann American students: a pilot study. apilot students: American toenvironmental intervention prevent excess weight gain in African- An DA. Williamson Dand Ryan M, Sothern CM, Champagne L, Lewis TM, Stewart CK, Jr., AntonSD, H, RL, Han Martin Newton human SIRT1 expression. expression. SIRT1 human nucleotide variation in a p53-binding sitenutrient-sensitive affects F, Mehdi Asingle- T, YR, K. Kim Irani Rankinen Eand Kumar S, Ravussin SB, Jung CS, Kim Yamamori A, T, J, DeRicco Kumar Hoffman A, TA, Naqvi Nutr pathways Leptin-signaling H. and leptin resistance. Munzberg weight? body human regulates that point set a for evidence there Is SB. Heymsfield Aand Bosy-Westphal MJ, Muller from Azores Islands. Islands. Azores from children in levels activity physical and obesity overweight, of Prevalence JA. Maia PT, and GP AT, Beunen Seabra SA, Pereira Katzmarzyk RG, Silva 95(8):4052-5. impaired insulin impaired of lipolysis. suppression with is associated tissue adipose obese human in oxygenation Reduced 2010; 63:123-32. 2010; PENNINGTON RESEARCH CENTER BIOMEDICAL Antiviral Res Diabetes Metab Res Rev Res Metab Diabetes 2010; 125(1):271-3 e1-5. 125(1):271-3 2010; 2010; 18(1):61-74. 2010; Ann Hum Biol Nat Neurosci Nat Hum Mol Genet 2010; 88(3):334-42. 2010; Am J Health Promot JHealth Am 2010; 37(5):682-91. 2010; 2010; 13(7):877-82. 2010; Neuroscience 2010; 19(21):4123-33. 2010; J Clin Endocrinol Metab Endocrinol Clin J 2010; 26(3):172-80. F1000 Rep Med Cell Physiol Biochem 2010; 166(1):61-72. 2010; 24(5):340-3. 2010; 2010; 2010; Med JMol Int Am JPathol Am 2010; 2:59. 2010; Analyst Forum Forum 2010; 2010;

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Diabetologia 2010; 95(1):88-91. 2010; Ann Med 2010; 5(2):103-10. 2010; 225(2):429-36. 2010; Physiol J Cell 2010; 1802(11):1020-7. Birth Defects Res A Clin Mol Teratol 2010; 3(6):531-8. SCIENTIFIC REPORT SCIENTIFIC 2010; 40(3):294-301. Pharmacol Biochem Behav Pharmacol 2010; 170(2):146-54. 2010; 7:22. 2010; Curr Stem Cell Res Cell Ther Stem Curr Ann BehavAnn Med athletes using a four compartment model as the reference method. (Lond) Metab Feitosa CM,Santos Saldanha IM, de GB, Freitas RL, Souza da Silva EP, R, Ada Feng D and Tome de Freitas RM. EffectsGF, of ubiquinone on enzymatic antioxidant and hydroperoxide concentration activities the in hippocampusrat during pilocarpine-induced seizures. 1315:33-40. R, Ada Santos IM, de Feitosa Freitas Feng Tome CM, D, de Souza GF, RM and Jordan Lipoic J. acid blocks seizures induced pilocarpine by via increases in delta-aminolevulinic K+-ATPase dehydratase and Na+, activity in rat brain. Sarzynski MA, Sternfeld Rankinen B, Grove ML, Fornage T, M, Jacobs DR, Sidney S and Bouchard Jr., C. Association of single-nucleotide polymorphisms candidate genes from with baseline 17 symptom- limited exercise test duration and decrease in duration years: 20 over the Coronary Artery Risk Development Adults (CARDIA) in fitness Young study. Circ Cardiovasc Genet Shevalye P and Obrosova Watcho H, Maksimchyk IG. Poly(ADP-ribose) Y, gene deficiency alleviates diabeticpolymerase-1 kidney (PARP-1) Acta Biophys Biochim disease. Santos DA, Silva AM, Matias CN, Fields DA, HeymsfieldSB and Sardinha LB. Accuracy of DXA in estimating body composition changes in elite Ruchat SM, Rankinen T, Weisnagel SJ, Rice T, Rao DC,Ruchat BergmanWeisnagel SJ, SM, Rice T, Rankinen RN, T, Bouchard C and Perusse L. Improvements in glucose homeostasis in response regular to exercisePro12Ala are influencedby thePPARG variant: resultsStudy. Family from the HERITAGE 53(4):679-89. Ruchat MC, SM, Bouchard Vohl Weisnagel SJ, Rankinen C and Perusse T, L. Combining genetic markers and clinical risk factors improves the risk impaired of assessment metabolism. glucose 206. Ruiz JC, Sherwood Ludlow JW, G, X and Gimble Wu S, Yu JM. Differentiated human adipose-derived cells stem exhibit hepatogenic capability in vitro and in vivo. Ryan DH. Approaching an employee about her weight. 2010; 82(5):524. Ryan DH, Johnson Myers WD, VH, Prather TL, McGlone MM, Rood J, Bray GA, Barootes GuptaBrantley BG, AK, PJ, Elkins BL, Broussard AP, Gaudin DE, Savory RL, Brock Datz RD, G, Pothakamuri SR, McKnight GT, Nonsurgical weight loss forStenlof K and extreme Sjostrom LV. obesity in primary care settings: results of the Louisiana Obese Subjects Study. Med Intern Arch Ryan DH and Kushner R. The state of obesity and obesity research. 2010; 304(16):1835-6. Salbaum JM and KappenC. Neural tube defect genes and maternal diabetes during pregnancy. 88(8):601-11. Salgado AJ, Reis RL, Sousa NJ and Gimble JM. Adipose tissue derived stem secretome: cells soluble factors and their roles in regenerative medicine. Samuel-Hodge Ard LP. JD Gizlice and Svetkey Z, CD, Cai Brantley J, PJ, functioningFamily and weight loss in a sample of african americans and whites.

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PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON 9(1):32-9. Redmon JB, Glick Bertoni H, Connelly AG, S, Glasser Feeney PA, SP, Hesson LA,Greenway Lawlor F, MS, Montez M and Montgomery B. Effect of the look AHEAD study intervention on medication use and related cost treat to cardiovascular disease risk factors in individuals with type 2 diabetes. 2010; 34(9):1427-33. Proietto Rissanen J, A, Harp JB, Q, Erondu Suryawanshi N, Yu S, Jones ME, Johnson-LevonasHeymsfield AO, SB, Kaufman KD and Amatruda JM. A clinical trial assessing the safetyinverse and efficacy agonistCB1R of the taranabant in obese and overweight patients: low-dose study. (Lond) Qualls-Creekmore M and Holmes E, Tong GM. Gastric emptying of enterally administered liquid meal in conscious rats and during sustained anaesthesia. Rankinen T, Roth SM,Rankinen Bray MS, Loos R, T, Perusse L, Wolfarth B, Hagberg JM and Bouchard C. Advances in exercise, fitness, and performance genomics. Redman LM and Ravussin E. Lorcaserin for the treatment of obesity. 46(12):901-10. 2010; (Barc) Drugs Today Redman LM, Rood JD, Veldhuis Smith J, SR, Williamson Ravussin E and D, ThePennington effect CT. of caloric restriction interventions on growth women. and men nonobese in secretion hormone Pistell PJ, Morrison Gupta CD, S, KnightPistell PJ, Keller AG, JN, Ingram DK and Bruce-Keller AJ. Cognitive impairment following high fat diet associated with brain is inflammation. consumption 2010; 219(1-2):25-32. 2010; Henagan TM, Echlin H, Boudreau A,Plaisance Hill KL, EP, Lenard NR, Hasek BE, Orentreich and N Gettys TW. Role of beta-adrenergic receptors in the hyperphagic and hypermetabolic responses dietary to methionine restriction. 299(3):R740-50. Primeaux Barnes SD, MJ, Braymer HD and Bray GA. Sensitivity the to satiating effects of exendinis 4 decreased in obesity-prone Osborne- Mendel rats compared obesity-resistant to rats. S5B/Pl Qualls-Creekmore M and Holmes E, Tong GM. Time-course of recovery of gastric emptying and motility in rats with experimental spinal cord injury. Motil Neurogastroenterol Racette SB, Lin X, Lefevre M, Spearie CA, Most MM, Ma L and Ostlund DoseRE, effects Jr. of dietary phytosterols on cholesterol metabolism: a controlled feeding study. ArgyropoulosRankinen Rao DC T, and G, Bouchard Rice a is T, C. CREB1 strong genetic predictorof the variation in exercise heart rate response Study. regularto Family exercise: the HERITAGE 3(3):294-9. 2010; Teran-Garcia M, Rice Rao T, Rankinen DC and Bouchard T, C. FTO genotype associated is with exercise training-induced changes in body composition. Ravussin E. The presence and role of brown fat in adult humans. Rep Diab 26(8):1018-19. Replogle WH, Barrett GR and Johnson Outcome WD. evaluation of reconstructionACL using patellar tendon allograft. Journal ArticlesJournal 2010 106

PUBLICATIONS 2010 Journal Articles nucleus accumbens. accumbens. nucleus the in antagonism receptor mu-opioid by chronic behavior reward food of suppression Reversible HR. AC, PJ,Shin Pistell Berthoud CB and Phifer Pharmacol model. rat streptozotocin-diabetic long-term in disease kidney (PARP) of polymerase manifestations multiple counteracts inhibition Poly(ADP-ribose) IG. Obrosova Band Slusher Y, Floyd EZ, VR, Drel Xu W, R, Maksimchyk S, Stavniichuk H, J, Lupachyk Zhang Shevalye health 2003. health children’s of survey national youth: U.S. in overweight and activity, physical PT. time, Screen ST, Katzmarzyk and Broyles BL SB, Sisson Baker lifespan. across the differences muscle skeletal SB. Ethnicity-related Heymsfield and LB D, Wang Sardinha Gallagher Z, W, Shen M, AM, Heo Silva 31(10):737-41. athletes. judo elite in changes power and composition Body LB. Sardinha and SB Heymsfield DA, Fields AM, Silva 44. men. of study aprospective in diabetes 2 type of incidence the and fitness cardiorespiratory activity, Physical SN. Blair and GA Hand A, McClain TS, Church DC, Lee X, Sui JC, Sieverdes surgery. Endocrinology bypass gastric Roux-en-Y of model arat in responses hormone induced Meal- HR. Berthoud and DL Townsend Sigalet H, AC,Shin Zheng RL, 59(9):1358-64. humans. in flexibility to metabolic status diabetes and race of contribution WT. The Cefalu and WD Johnson JE, Galgani Stull AJ, women. and men resistant insulin- in obese, improve insulin in sensitivity blueberries Bioactives WT. Cefalu and CM WD, Champagne KC, Johnson Cash Stull AJ, Exerc women. C-reactive on among protein activity physical of doses different CP,of Earnest Effects LK, TS. Church SN and Stewart Blair 49(6):1036-45. 2010; neuropathies. and diabetic prediabetic peripheral and stress 12/15-lipoxygenase of nitrosative in Role IG. Obrosova and JL Stevens VareniukNadler I, H, MJ, Shevalye VR, Drel R, Stavniichuk Stem Cells Dev cells. stem mesenchymal ear adult of immunophenotype and frequency, differentiation characteristics, B. Cell growth and Gawronska-Kozak JM TP, Gimble J, Frazier ES, Chiu BA, Bunnell BG, Staszkiewicz Rowan Sci Sci Med ABiol J Gerontol rats. Fischer-344 in span life and supplementation Metformin DB. Allison and DK Jr., Ingram DL, GS, ElamCF,Smith Roth MA, Lane JA, Jr., Mattison 3(10):38-47. Macaques. Rhesus male of axis neuroendocrine reproductive the on restriction calorie HF. moderate of Urbanski Impact and MA Ottinger GS, Roth DK, Ingram JA, BD, Mattison Sitzmann (Macacamulatta).macaques rhesus young in characteristics semen and production testosterone on restriction calorie moderate of Effects MA. Ottinger and MB Zelinski HF, Urbanski GS, Roth DK, Ingram JA, Mattison EH, BD, Leone Sitzmann syndrome. PT.Katzmarzyk Accelerometer-determined steps/day and metabolic and WD Tudor-Locke TS, Johnson Church C, SM, Camhi SB, Sisson SCIENTIFIC REPORT 2010; 42(4):701-7. 2010; 2010; Biol JHum Am 22(1):76-82. 2010; 79(7):1007-14. 2010; 2010; 38(6):575-82. 2010; JPrevAm Med J Adolesc Health Adolesc J 2010; 19(1):83-92. Neuroscience 2010; 151(4):1588-97. 2010; 2010; 65(5):468-74. 2010; 2010; 140(10):1764-8. 2010; J Nutr 2010; 47(3):309-11. 2010; Biol Reprod

2010; 170(2):580-8. 2010; 2 010–2011 Br J Med Sports 2010; 83(4):635-40. Int J Med Sports 2010; Longev, 2010; Sci, Open Free Radic Biol Med 2010; 44(4):238- 2010; Metabolism 2010; 2010; Med Sci Sports Sci Sports Med Biochem 2010; 2010;

prone OM rats. rats. OM prone than placeshowed the greaterpreference obesity- conditioned cocaine S5B rats Volkow and JK ND. Obesity-resistant Robinson Wang GJ, GA, SD, Bray Primeaux BJ, Anderson M, J, Michaelides Cho R, Kim PK, Thanos 31. tissue gain overfeeding. with adipose of mechanisms in cellular differences MD. Regional Jensen during weightduring loss. intake energy determine to model A computational SB. Heymsfield JA and Levine CK, Martin LA, Redman DA, Schoeller DM, Thomas equations. balance energy physiological in use for model fatmass - mass free fat New SB. Heymsfield and BJ Strauss A, McDougall L, Mayer CK, Martin JA, Levine D, SK, Das Thomas cells. stem adult tissue-derived adipose for acryoprotectant as polyvinylpyrrolidone RV. of Evaluation Devireddy and JM Gimble Wu S, X, Thirumala 2010; Med Eng Regen Tissue 4(3):224-32. medium. freezing tissue of in adipose a serum-free fraction vascular stromal of RV. Cryopreservation Devireddy and JM Gimble S, Thirumala Dev Cells cells. stem adult adipose-derived of cryopreservation for media freezing aserum-free in cryoprotectants the as sulfoxide dimethyl and RV. methylcellulose of Evaluation Devireddy and JM Gimble S, Thirumala transgenic mice through elevated energyexpenditure. in NF-kappaB by resistance insulin and inflammation of Uncoupling Ye Zand Gao M, J. Keenan RJ, Martin R, Mynatt JK, Kim H, Ko Ong HJ, DY, Jung B, Tang J, Gawronska-Kozak T, J, Staszkiewicz J, Yin Zhang physiology before obesity. circadian altered with offspring in results mice C57BL/6J in pregnancy during malnutrition Protein AA. AV Centanni Butler GM, and Sutton cycle. the light during feeding entrainment during to restricted metabolism synchronizing for are required receptors melanocortin-3 system RP, McMillan nervous R, Central MW, Hulver AA. Tschop Butler and MH Perez-Tilve D, JM, Nogueiras Kumar KG,Gimble K, Begriche GM, Sutton Tchoukalova and YD, TchkoniaJL SB, Votruba T, Kirkland N, Giorgadze Spring) differences in adipogenesis innormal-weight humans. depot-dependent MD.and Sex- Jensen and T, JL Thomou Kirkland Tchkonia SB, Votruba C, Tchoukalova T, YD, Koutsari N, Giorgadze model. bypass Roux-en-Y a in adaptation intestinal on hormones trophic systemic and secretions, biliary-pancreatic nutrients, of influence The DL. Sigalet JJ and Holst HR, Berthoud H, Zheng PK, Taqi Chelikani E, Wallace Heuvel E, de LE, 285(7):4637-44. biologic factors. in relation to adiposity-related measures of adiposity anthropometric and absorptiometric x-ray dual-energy of FB. Comparison Hu and WC Willett SB, D, Heymsfield Spiegelman RM, Dam van Q, Sun Spring) independent correlate BMI. of in errors self-reported PT. is an Waist circumference Katzmarzyk and WD Johnson R, Sullivan 2010; 42(5):872-8. Exerc Sci Sports Med mortality. cancer lung on fitness cardiorespiratory of Influence SN. Blair CD and Lee TS, Church JR, Hebert AdamsSA, CE, Matthews DC, Lee X, Sui FASEB J FASEB 2010; 18(10):1875-80. 2010; 18(11):2237-9. 2010; PENNINGTON RESEARCH CENTER BIOMEDICAL 2010; 19(4):513-22. 2010; 2010; 24(3):862-72. Physiol Behav Am J Epidemiol 2010; 16(4):783-92. CMethods Eng Part Tissue 2010; 92(6):1326-31. 2010; Nutr JClin Am 2010; 45(5):987-95. Surg 2010; J Pediatr Endocrinology 2010; 101(5):713-8. 2010; Proc Natl Acad Sci USA Acad Natl Proc 2010; 172(12):1442-54. 2010; Nutr Metab (Lond) 2010; 151(4):1570-80. 2010; 2010; 107(42):18226- 2010; Obesity (Silver Obesity Obesity (Silver Obesity J Biol Chem J Biol 2010; 7:39. 2010; Stem Stem 2010; 2010; J PUBLICATIONS 107

2010; Med 2010; 2010; 2010; Curr Diab Rep Diab Curr 010–2011 2 Am J Primatol

2010; 55(9):2619-35. | Brain Behav Immun Behav Brain

Behav Brain Res Brain Behav Mediators Inflamm Mediators Congest Heart Fail Congest 2010; 100(4):408-16. 2010; 33(4):305-14. J Behav Med 2010; Phys Med Biol Med Phys 2010; 22(4):476-83. Am J Hum Biol 2010; Am J Clin Nutr 2010; 92(6):1369-77. 2010; 34(9):1355-64. Physiol Behav Physiol SCIENTIFIC REPORT SCIENTIFIC 2010; 51(6):1416-23. Int J Obes (Lond) J Am Cardiol Coll 2010; 56(14):1140-8. J Lipid Res diet-induced neuropathy of prediabetes and obesity: effectPMI-5011, of an ethanolic extract of Artemisia dracunculus L. 2010:268547. White CL, Purpera MN, Ballard K and Morrison Decreased CD. food following overfeedingintake leptin-dependent involves leptin- and independent mechanisms. DL, JM, IngramWu Takahashi DK, Mattison JA, Roth G, Ottinger MA and Zelinski MB. Ovarian reserve tests and their utility in predicting response controlledto ovarian stimulation in rhesus monkeys. Williamson Han H, Champagne DA, Anton SD, CM, Allen R, LeBlanc E, Ryan DH, McManus K, Laranjo Loria CM, N, Bray Carey GA and VJ, Sacks FM. Adherence a multi-dimensional is construct in the POUNDS LOST trial. J Behav Med 2010; 33(1):35-46. Williamson Han H, Champagne DA, Anton SD, CM, Allen R, Leblanc E, Ryan DH, Rood McManus J, K, Laranjo Loria CM, N, Bray Carey GA VJ, and Sacks FM. Early behavioral adherence predicts short and long-term weight loss in the POUNDS study. LOST Williams JS, PandarinathanWood L, JT, Janero DR, Lammi-Keefe CJ and Makriyannis A. Dietary docosahexaenoic acid supplementation alters and brain in metabolites select endocannabinoid-system physiological plasma. Wang Y and HuWang G. Individual and joint associations of obesity and physical activity on the risk of heart failure. 16(6):292-9. Antikainen R, Mahonen M, Jousilahti J, P, Tuomilehto Y, Wang Katzmarzyk PT and Hu G. Occupational, commuting, and leisure-time physical activity in relation heart to failure among finnish men and women. Z, HeymsfieldWang SB, Chen ZhuZ, S andPierson RN. Estimation of percentage body fat dual-energy by x-ray absorptiometry: evaluation by composition. elemental human vivo in Z, HeymsfieldWang SB, Ying PiersonZ, GallagherRN, Jr., D and Gidwani S. A approach predicting level to cellular resting energy expenditure: Evaluation of applicability in adolescents. 72(8):672-80. Hsuchou H, Kastin X,Wu He AJ, Y, Rood Essential JC and role W. Pan of receptor in normal anxietyinterleukin-15 behavior. 2010; 24(8):1340-6. X,Wu Kastin AJ, The Hsuchou effects H and W. Pan of IL2Rgamma knockout on depression and contextual memory. 213(2):319-22. Wang Z, YingWang Z, Bosy-Westphal A, Zhang Schautz J, B, Later W, HeymsfieldSB and Muller Specific MJ. metabolicratesmajor of organs mechanistic adulthood: across by model tissues evaluation and of resting energy expenditure. and Cefalu Current ZQ conceptsWang WT. about chromium supplementation in type 2 diabetes and insulin resistance. 2010; 10(2):145-51. Zhang XH, EZ Floyd and Cefalu Human adenovirus WT. Y, Yu ZQ, Wang decreases36 fatty acid oxidation and increases de lipogenesis novo in primary cultured human skeletal muscle promoting by cells Cidec/FSP27 expression. Sui X, Church TS and Blair SN. Sedentary Barry HookerWarren TY, SP, V, behaviors increase risk of cardiovascular disease mortality in men. SportsSci Exerc 42(5):879-85. 2010; Stavniichuk R, Ribnicky DM, P, RaskinWatcho I and Obrosova IG. High-fat

Am J J Neurochem J Int J Behav Nutr Res Q Exerc Sport Med Sports Sci Exerc 2010; 164(1):46-52. 2010; 12(2):167-77. Current gerontology and and gerontology Current Curr SportsCurr Med Rep 2010; 2010; 18(12):2301-10. Integrative biology : quantitative quantitative biology : Integrative 2010; 5(1):51-5. 2010; 4(4):271-76. 2010; 2(7-8):346-53. Diabetes Obes Metab Obes Diabetes Arch PediatrArch Adolesc Med 2010; 24(7):2281-91. 2010; J Appl 2010; Physiol 108(6):1487-96. FASEB J Obesity (Silver Spring) Obesity (Silver 2010:219683. Int J Pediatr Obes 2010; 35(4):580-7. 2010; 39(4):e13-20. 2010; 2010; 7:60. 2010;

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON 2010; 42(12):2244-50. 2010; Uranga RM, Bruce-Keller AJ, Morrison Fernandez-Kim CD, Ebenezer SO, Zhang L, Dasuri K and Keller JN.PJ, Intersection between metabolic dysfunction, brain and aging. fat diet consumption, high Tian Z, Ye T, Zhang X, Liu E, Wang W, Wang P, Liu G, Yang X, Liu Hu G, and G Yang P, Wang Zhang X, Liu W, E, Wang T, Tian Z, Ye Z. SleepYu duration and hyperglycemia among obese and nonobese children aged 6 years. 3 to Prev Med C, JohnsonTudor-Locke WD and Katzmarzyk Accelerometer- PT. determined steps per in U.S. day children and youth. Tudor-Locke C, JohnsonTudor-Locke WD and Katzmarzyk Frequently reported PT. activities intensity by for U.S. adults: the American Time Use Survey. Tudor-Locke C. better to Steps Tudor-Locke cardiovascular health: steps how many does ittake achieve to good health and how confident arewe in this number? Curr Cardiovasc Risk Rep C. A shortTudor-Locke list about what I do and don’t know: about activity. physical of objective monitoring 9(2):71-2. C, BrashearTudor-Locke MM, Johnson WD and Katzmarzyk PT. Accelerometer profiles of physical activity and inactivity in normal weight, overweight, and obese U.S. men and women. Phys Act 2010; 81(1):97-101. RosenstockVilsboll Yki-Jarvinen J, T, Luo E, Musser H, Cefalu Chen Y, WT, Kaufman Amatruda KD, LingB, JM, Andryuk Y, Engel and SS Katz PJ, L. Efficacy and safety of sitagliptin when added toinsulin therapy in patients with type 2 diabetes. Fujioka S, K, GantzWadden TA, Toubro I, Erondu NE, Chen M, Suryawanshi S, Carofano Johnson-Levonas W, Shapiro DR, AO, Kaufman HeymsfieldKD, SB and Amatruda JM. A randomized trial of lifestyle modification achieved with loss weight and maintaining for taranabant low-calorie a diet. Kang LQ, Wan SM, Eng G, Grayson WL, XL, Lu Huo B, Gimble Guo J, XE, andMow Vunjak-Novakovic VC G. Geometric controlof human stem cell morphology and differentiation. macro to nano from biosciences 2010; 114(2):344-61. Uranga RM and Keller JN. Diet and age interactions with regards to cholesterol brain regulation and pathogenesis. geriatrics research C, Marshall Tudor-Locke AL, Craig C, Hagstromervan der Ploeg M, HP, Sjostrom M and Bauman A. Reliability and validity of the international activityphysical questionnaire for walking. assessing Tompkins CL, Cefalu W, Ravussin CL, E, Goran CefaluTompkins W, M, Soros A, Vargas J, Volaufova A and Sothern MS. Feasibility of intravenous glucose tolerance testing prior puberty. to Timmons JA, Knudsen Koch Sarzynski LG, S, Rankinen T, Keller M, Jensen T, Scheele C, NB, Vollaard Nielsen MacDougald S, AkerstromP, OA, Jansson T, TarnopolskyE, GreenhaffPL, MA, van Loon Pedersen LJ, BK,Sundberg CJ, Wahlestedt C, Britton andSL Bouchard C. molecular Using classificationto predict maximal in aerobic capacity gains following endurance exercise training in humans. AR, Feng D andTome Freitas RM. The effects of alpha-tocopherol on hippocampal stress pilocarpine-induced in prior oxidative to seizures. Res Neurochem H, Hsuchou H, Kastin UniqueTu AJ, leptin X and trafficking W. Pan Wu by a tailless receptor. Journal ArticlesJournal 2010 108

PUBLICATIONS 2010 Journal Articles Cytotherapy analyzes. mRNA adipogenic and cytometric cells by flow stem adipose-derived human subcutaneous of characterization and Yield JM. Gimble and AA Polk P, MA, Ptitsyn Yu Dietrich Wu LK, X, G, Scott Med Ann Behav life. of quality health-related on interventions the PREMIER of Effects V, PJ. Myers GJ, Brantley SE and Chae J, Jerome Young Coughlin DR, involution. thymic age-related promotes and adipogenesis ectopic induces treatment and constitutive-PPARgamma activation Thiazolidinedione VD. Dixit and T Leff Youm SR, Smith Yang YH, R, Amin H, 2(6):361-8. 2010; feedback response in obesity and calorie restriction. restriction. calorie and obesity in response feedback a by inflammation: metabolism energy of Ye Regulation JN. Keller Jand inflammation and insulin resistance. systemic for implications diversity: and compromisesTCR repertoire tissueadipose from T cells mediators of proinflammatory production the increases VD. Obesity Dixit F, and RL Mynatt JM, Stephens Greenway JM, Gimble Yang A, Youm Ravussin H, B, Vandanmagsar YH, (Engl) J Med phosphoenolpyruvate carboxykinasetranscription by NF-kappaB. of inhibition mediates CREB and NF-kappaB between corepressor Yan ZG, Ye Gao JH, Weng JP. and JP anuclear of Exchange behavior. and expression, gene and plasticity cellular bulb olfactory of alterations cocaine-induced mitigates MP. restriction Mattson and JL Dietary Cadet GR, Uhl LE, Niklason B, KG,Ladenheim Becker S, Chigurupati F, Hall Scott MR, MT,Xu Perona Mughal X, PJ, Pistell JD, Lathia 151(6):2504-14. inflammation. and mobilization lipid of mice: arole SIRT1+/- the in steatosis to liver leads 1) Sirtuin (Mammalian activity Xu J, Ye Weng Jand Yin F, SIRT1 of J. Lack CA, J, Rivera Zhang Z, Gao barrier. blood-brain the of cells IL15Ralpha novel of endothelial signaling cerebral in variants splicing PanWu W, X, and Stone KP, Expression Y,AJ. Zhang Kastin Hand Hsuchou encephalomyelitis. autoimmune experimental in effects and beneficial shows upregulation interleukin-15 Cerebral PanWu W, X, Y,AJ. He Kastin Hand Hsuchou SCIENTIFIC REPORT J Neurochem Aging Cell 2010; 123(2):221-6. 2010; 12(4):538-46. 2010; 40(3):302-12. 2010; J Neuroimmunol 2010; 9(4):478-89. 2010; 114(1):323-34. 2010; J Neurochem

2 2010; 223(1-2):65-72. 010–2011 J Immunol 2010; 114(1):122-9. 2010; 185(3):1836-45. 2010; Endocrinology Aging (Albany NY) 2010; 2010; Chin Chin

the factor Xa inhibitor apixaban in rabbits. rabbits. in apixaban inhibitor Xa factor the of pharmacodynamics and pharmacokinetics Metabolism, Wongand PC. JM Luettgen B, Xin B, He EJ, Crain Wang N, L, Raghavan D,Zhang K, He Mexican fruit fly (Anastrepha ludens). (Anastrepha fly fruit Mexican the in dependent diet are extract cranberry and oregano an of effects P, Prolongevity Liedo R. Yu JR, Carey Ghaedian S, Zou Band DK, Ingram simulated observations. computer- and real-world of concordance crashes: vehicle motor following injury body upper serious of risk and BMI DB. Allison and PW, F, Laud Pintar A, Shih X, Ma SB JE, W, Kim Shen Zhu S, Heymsfield 42(8):1519-27. 2010; Exerc Sci Sports Med HR. maximum exercise symptom-limited of examination age-predicted Longitudinal Jr. DR, Jacobs and WL Haskell C, Bouchard RS, Crow CE, Lewis MR, PJ, Schreiner B, Sternfeld S, Carnethon Sidney JR, Suarez-Lopez Zhu N, Chem Food Agric elegans. in acids intestinal reduce Caenorhabditis deposition fat fatty short-chain and starch, resistant fermented starch, Resistant R. Martin and JM King A, Prudente R, Manaois CR, Gissendanner RN, Senevirathne F, J, Enright Zheng J, J,Ye Zhou Finley M, F, Keenan J, Greenway Res accumbens is by inhibited Y1R-blockade and MC3/4R- stimulation. nucleus the of activation by mu-opioid induced intake High-fat HR. Berthoud CB and Phifer LM, TownsendAC, Shin H, Patterson Zheng RL, Physiol Regul Integr Comp Physiol intake. of food suppression in leptin-induced projections POMC hypothalamomedullary for role Apotential HR. Jr.MG, Berthoud and KP, GW, Louis CJ, Skibicka Myers HJ, Rhodes Grill LM, Patterson H, Zheng 39(6):1196-211. Communications in Statistics - Simulation and Computation 2010; continuous distributions. zero-inflated with of populations equality for tests six of study WD. Empirical Wu Johnson L, Jand Zhang inhibitor treatment. J Neurochem cells by proteasome neural in PERKkinase of Activation JN. Keller Y and SO, Liu Fernandez-Kim AJ, PJ, Bruce-Keller Ebenezer L, Zhang K, Dasuri 29(1):70-80. 65(1):41-50. 2010; 1350:131-8. 2010; PENNINGTON RESEARCH CENTER BIOMEDICAL 2010; 58(8):4744-8. 2010; PLoS Med PLoS 2010; 298(3):R720-8. 2010; 112(1):238-45. 2010; 7(3):e1000250. 2010; J Gerontol A Biol Sci Med Sci Sci Med ABiol J Gerontol J Thromb Thrombolysis Am J Am 2010; 2010; 2010; 2010; Brain J PUBLICATIONS 109

J Exerc 2011; 2011; 2011; 2011; 010–2011 2 J Appl Physiol Pediatrics

Psychiatr Clin North Am Obesity (Silver Spring) Obesity (Silver 2011; 12(11):1813-26. 2011; 104(4):546-54. 2011; 2011; 300(6):R1266-77. 2011; 2011; 21(6):888-96. 2011; 2011; 286(47):40771-81. 2011; Med Clin North Am Clin Med 2011; 38(2):150-8. Comprehensive Physiology Comprehensive J Biol Chem Physiol Behav Physiol 2011; 105(1):106-19. 2011; 104(1):29-39. Curr Opin Neurobiol Opin Curr SCIENTIFIC REPORT SCIENTIFIC Expert Opin Pharmacother Health Educ Behav Educ Health 2011; 306(8):828-30. 2011; Physiol Behav Physiol Physiol Behav Physiol 2011; 23(4):665-71. JAMA 2011; 39(4):212-7. 2011; 21(2):146-50. 2011; Am Regul J Physiol Integr Comp Physiol Bellanger DE and Greenway FL. Laparoscopic sleeve gastrectomy, 529 cases without a leak:short-term results and technical considerations. Obes Surg Berthoud HR. Metabolic and hedonic drives in the neural control of appetite: who the is boss? Berthoud HR, Lenard NR and Shin Food AC. reward, hyperphagia, and obesity. Berthoud HR and Munzberg H. The lateral hypothalamus as integrator of metabolic and environmental needs: from electrical self-stimulation to opto-genetics. Berthoud HR, and Zheng Shin AC H. Obesity surgery and gut-brain communication. Billes and SK Greenway FL. Combination therapy with naltrexone and obesity. for bupropion Begriche K, Sutton GM and Butler AA. Homeostastic and non- homeostatic functions melanocortin-3 of of receptors control the in metabolism. and balance energy Bouchard C. Overcoming barriers progress to in exercise genomics. Sport Rev Sci Bouchard C, Rankinen T and Timmons JA. Genomics and genetics in the biology of adaptation exercise. to Barreira Harrington TV, DM, Staiano AE, Heymsfield SB and Katzmarzyk Body adiposityPT. index, body mass index, and body fat in white and black adults. Bartfield VJ, Jerome JK, GJ, BC, Batch KennedyStevens Vollmer BM,WM, HarshaAppel D, LJ, Desmond R and Behavioral Ard JD. transitions and weight change patterns within the PREMIER trial. 2011; 19(8):1609-15. Baruth M, Lee DC, Sui X, Church TS, Marcus BH, Wilcox S and Blair SN. Emotional outlook on life predicts increases in physical activity among inactiveinitially men. Begriche K, Levasseur PR, Zhang Rossi J, Skorupa Solt J, LA, D, Young Marks DL, MynattB, Burris TP, RL and Butler AA. Genetic dissection of functionsthe seven-transmembrane a melanocortin-3 the of receptor, G-protein-coupled suggests receptor, roles for central and peripheral receptors in energy homeostasis. 127(5):e1272-9. Bray GA. Medications for weight reduction. 1:1603-48. Bouchard C, Sarzynski MA, Rice TK, Kraus WE, Church TS, Sung YJ, Rao DC Genomic and Rankinen predictors T. of the maximal O uptake response standardized to exercise training programs. 110(5):1160-70. X, Klebanoff Tian Z, Yang Z, Liu E, Yu T, M, Ye Bowers K, Liu G, P, Wang E, Hu G and Zhang C.Yeung Birth weight, postnatal weight change, and risk for high blood pressure among chinese children. 95(5):989-1008. Bray GA and Ryan DH. Drug treatment of obesity. 34(4):871-80. 2011; Brouillette RM, Martin CK, Correa JB, AB, Davis Han H, Johnson Foil WD, HC, Hymel A and Keller JN. Memory for names test provides a useful confrontational naming task for aging and continuum of dementia. Dis Alzheimers

2011; 2011; Obes Rev PLoS One 2011; 2:150-54. J Clin Endocrinol Metab 2011; 74(3):496-503 e3. 74(3):496-503 2011; Basic Res Cardiol Res Basic J Phys ActJ Phys Health 2011; 286(13):11659-71. 2011; 63(8):1111-8. Psychology 2011; 11(2):215-23. 2011; 26(1):143-55. 2011; 2011; 106(4):1822-32. 2011; J Biol Chem Gastrointest Endosc 2011; 129(7):1611-23. J Alzheimers Dis J Pharm Pharmacol Pharm J J Neurophysiol J Int J Cancer 2011; 108(10):1438-42. 2011;

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Araujo JA, Greig NH, Ingram DK, Sandin de J, Rivera C and Milgram NW. Cholinesterase inhibitors improve both memory and complex learning in aged beagle dogs. Artero EG, Lee DC, Ruiz JR, Sui X, Ortega Church FB, TS, Lavie CJ, Castillo MJ and Blair SN.prospective A study of muscular strength and all-cause mortality in men with hypertension. J Am Cardiol Coll 2011; 57(18):1831-7. Arvidsson M, Fitch Hudes D, C and Fleming ML, SE. Tudor-Locke Accelerometer response physical to activity intensity in normal-weight versus overweight African American children. Adarkwah and Gandjour CC A. Cost-effectiveness of angiotensin- converting enzyme inhibitors nondiabetic in advanced disease. renal Expert Rev Pharmacoecon Outcomes Res Adarkwah Gandjour CC, A, Akkerman M and Evers SM. Cost-effectiveness angiotensin-convertingof enzyme of inhibitors for prevention the diabetic nephropathy in The Netherlands--a Markov model. Abuznait AH, Cain C, Ingram Burk D and D, KaddoumiA. Up-regulation beta of accumulation amyloid: P-glycoprotein reducesof intracellular investigation of P-glycoprotein as a novel therapeutic target for Alzheimer’s disease. LifestyleAdams CE, Greenway and Brantley FL PJ. factors and ghrelin: critical review and implications for weight loss maintenance. 2011; 12(5):e211-8. Adams CE, Myers VH, Barbera The role B and of fear Brantley of PJ. negative evaluation in predicting depression and quality of life four years after bariatric surgery in women. 2011; 6(10):e26139. 2011; Agarwal MA, Welsch D, Keller JN and Chronic Francis J. exercise modulates RAS components and improves balance between pro- and anti-inflammatory cytokines in the brain SHR.of 106(6):1069-85. Aggarwal S, Ndinguri Solipuram MW, R, Wakamatsu N, Hammer RP, releasing hormone [DLys(6)]-luteinizing W. Hansel and D Ingram hormone-curcumin conjugate inhibits pancreatic cancer cell growth in vitro and in vivo. 8(5):682-92. Austgen JR, Hermann GE, Dantzler HA, Rogers RC and Kline DD. Hydrogen sulfide augments synaptic neurotransmission in the nucleus of the solitary tract. Ainsworth BE, Haskell WL, Herrmann Meckes SD, N, Bassett DR, Jr., C, GreerTudor-Locke JL, Whitt-Glover J, Vezina MC and Leon AS. 2011 Compendium of Physical Activities: a second update of codes and MET Medvalues. Sports Sci Exerc 2011; 43(8):1575-81. Ajja R, Lee DC, Sui X,Church TS NB. and Usefulness Steven of serum bilirubin and cardiorespiratory fitness as predictors of mortality in men. Am JCardiol Zhang Ukropec J, Anunciado-Koza Bajpeyi J, S, Koza RP, RA, Rogers RC, InactivationCefalu Mynatt WT, of the mitochondrial RL and Kozak LP. transporter)carrier reduces (ATP-Mg2+/Pi physical SLC25A25 endurance and metabolic efficiency in mice. Abell TL, Johnson Kedar A, WD, Runnels JM, Thompson Weeks J, ES, Minocha A and Griswold ME. A double-masked, randomized, placebo- controlled trial of temporary endoscopic mucosalgastric electrical forstimulation gastroparesis. 2011; 96(4):1160-8. Bajpeyi S, Pasarica M, Moro C, Conley K, Jubrias S, Sereda Burk O, DH, Zhang Z, Gupta A, Kjems L and Smith SR. Skeletal muscle mitochondrial capacity and insulin resistance in type 2 diabetes. Journal ArticlesJournal 2011 110

PUBLICATIONS 2011 Journal Articles human adipose-derived stromal/stem cells. cells. stromal/stem adipose-derived human adherent passaging for products Use of animal JM. protein-free Gimble and RL Reis PP, ME, Gomes Carvalho IR, Dias M, Yu Wu X, Dietrich G, Organs Tissues Cells lipoaspirates. human from recovery stem cell adipose-derived on time storage of effect The JM. PP, Gimble and RL Reis Carvalho ME, Gomes Yu Wu IR, X, Dias G, for body contouring and spot fat reduction. reduction. fat spot and contouring body for therapy laser low-level of O, Efficacy Yu FL. Dubuisson Greenway Y and NV, Dhurandhar N, Mashtalir VK, Podichetty TS, Guillot MK, Caruso-Davis health. cardiometabolic Accelerometer-determined and moderate activity intensity lifestyle Tudor-Locke and PT C. WD, Katzmarzyk Johnson SB, Sisson SM, Camhi Health in US adults. activities lifestyle Accelerometer-determined Tudor-Locke and PT C. WD, Katzmarzyk Johnson SB, Sisson SM, Camhi 9(2):119-25. circumference. waist and index mass body of role risk: metabolic and atherosclerosis Subclinical GS. Berenson and W, C Chen PT, ST, SR, Bouchard Broyles Srinivasan Katzmarzyk SM, Camhi 159(2):303-7. adolescents. in index mass body and clustering factor risk PT. cardiometabolic of Prevalence Katzmarzyk SM and Camhi differences. race and sex fat: body total and subcutaneous, to visceral, BMI and circumference waist of PT. relationship The Katzmarzyk SR and Smith DH, Ryan E, Ravussin RL, WD, Newton Johnson FL, Greenway C, Bouchard GA, Bray SM, Camhi Aerobics Center Longitudinal Study. the from findings cancer: prostate of risk and fitness Cardiorespiratory SN. Blair CE and Matthews IM, Lee TS, Church JR, W, Hebert Byun X, Sui Health Act Canadian adults. and chronic among diseases guidelines activity physical meeting between PT. association The Katzmarzyk SN and Bryan Physical Education Days. J Hum Kinet Non- and Education, Physical Children’s Weekend, on Step Counts PW. Darst TA, Hand Brusseau Mars Tudor-Locke KulinnaPH, der van C, children. fifth-grade and fourth- of patterns activity physical segmented PW.Darst Pedometer-determined Hand Mars der van TA, M, Brusseau Tudor-Locke KulinnaPH, Ferry C, 13(1):11-4. Markesbery. to Dr. R. atribute William disease: Alzheimer’s of study the in Lessons JN. Keller and AJ Bruce-Keller Neurobiol Dis APPxPS1 to Abeta(1-42) is mice linked humanized in NOX activation. and MP,Murphy impairment Cognitive JN. Keller St Dand Clair Van LJ, Eldik Davis PR, JM, McMullen TL, AG, Beckett Knight S, Gupta AJ, Bruce-Keller 40(5):522-9. 2011; JPrev Am Med framework. active-living and into apark-use capital social KP, ST, Theall Integrating AL. Broyles AJ, Rung Mowen Jand Gustat Nutr groups. sex and ethnicity mass across relationship mass--fat-free fat of PT. Consistency Katzmarzyk SR and Smith DH, Ryan E, Ravussin RL, WD, Newton Johnson FL, Greenway GA, ST,Bray C, Broyles Bouchard 38(4):492-9. children. school Hawaiian of cohorts two in adiposity of Measures L. Allen and PT Katzmarzyk LA, Gotshalk DE, Brown SCIENTIFIC REPORT 2011; 105(8):1272-6. 2011; 8(3):382-9. 2011; 2011; 8(1):10-7. 2011; 2011; 44(3):317-26. Prev Med Prev Obesity (SilverObesity Spring)

2011; 52(5):358-60. 2 J Phys Act Health J Phys Act 010–2011 Cancer Epidemiol 2011; 27:125-35. 2011; 194(6):494-500. Metab Syndr Relat Disord Syndr Relat Metab Cytotherapy Obes Surg Obes Neuromolecular Med 2011; 19(2):402-8. 2011; 2011; 8(2):279-86. 2011; 2011; 35(1):59-65. 2011; Ann Hum Biol 2011; 21(6):722-9. J Pediatr 2011; 13(5):594-7. 2011; J Phys Act J Phys Act 2011; 2011; J Phys 2011; 2011; 2011; 2011; 2011; 2011; Br J Br inflammation and vascular pathologies. pathologies. vascular and inflammation tissue adipose between link acellular WT. Fractalkine: Cefalu Weight Loss Maintenance trial. trial. Weight Maintenance Loss the during maintenance and loss weight successful with associated intakes Dietary VH. LF, Myers Lien Cand BC, Loria A, Dalcin Funk KL, Batch PH, Lin EJ, ST, LD, KC, Broyles Levy Cash Moran CM, Champagne pilocarpine-induced seizures. seizures. pilocarpine-induced , K(+) -ATPase, after Mg(2+) and hippocampus rat in -ATPase activities lipoic acid and ubiquinone on delta-aminolevulinic dehydratase, Na(+) of effects J. Neuropharmacological Jordan Dand Feng RM, Freitas de 43(3):542-9. revisited. Womersley and Durnin groups: racial in skinfolds by percent fat Gallagher D. Predicting and SB Heymsfield RN, F, Silva-Palacios Z, Pierson Kaleem JC, Wang LE, J, Thornton Davidson rat neuronal and astrocyte cultures: implications for protein misfolding protein for cultures: implications misfolding rat and astrocyte neuronal primary in toxicity analog acid Amino JN. Keller and AJ SO, Bruce-Keller Fernandez-Kim L, PJ, Zhang E, Gavilan Ebenezer RM, K, Uranga Dasuri in toxicoproteomics. modifications revealsunexpected tagging Tabb WM and Sequence Gallagher DL. SR, Pennington BC, Collins DC, Liebler SG, Codreanu MC, Chambers S, Dasari 2011; 36(5):660-70. C-reactive protein in young, healthy females. lowers training resistance and Endurance J, Tuuri LK. Stewart Gand CP, Earnest M, LG, Winchester Zanovec TM, Johnson Henagan LA, Daray obesity. andwith theirassociations physical activity occupation-related in U.S. Trends C. over 5decades Bouchard SN and Blair CK, Martin RQ, Rodarte CP, PT, Earnest Tudor-Locke Katzmarzyk DM, C, Thomas TS, Church Dis Cardiovasc diabetes. and syndrome, metabolic T.Church obesity, in Exercise 2011; 17(9-10):1437-44. cocultures. of adipocyte/endothelial function Lipolytic DL. Kaplan Gand Vunjak-Novakovic JM, Gimble E, Bellas JH, Choi disinhibited eating in behavior adults. on is dependent gain weight and duration sleep short between JP,Chaput JP, Despres association Tremblay Cand The A. Bouchard cell One culture. PLoS in disposal NV. glucose improves that Dhurandhar E4orf1: ligand anovel Vand Hegde R, Krishnapuram N, O, Mashtalir Dubuisson EJ, Dhurandhar 364(12):1104-15. preventiondiabetes glucose tolerance. in impaired for Reaven PD. FB, Nand Musi Stentz Pioglitazone K, Williams RE, Ratner WJ, Mack AE, S, Mudaliar Kitabchi HN, Hodis RR, Henry TA, SC, Clement Buchanan GA, Bray Tripathy M, Banerji D, RA, DC, Schwenke DeFronzo Patient Couns Educ patients. 2diabetes type in behavior sedentary and activity physical on support telephone with program modification behavioral based pedometer- of a effects The IM. Bourdeaudhuij De and JM Kaufman JJ, Tudor-Locke CE, KP, Bouckaert JB, Greef Ruige De BI, Deforche trial. controlled randomized arm athree- patients: 2diabetes type Belgian in activity physical Increasing I. Tudor-Locke B, Bourdeaudhuij De Cand Deforche K, Greef De development of oxidative stress. differentiationand adipocyte differentiationto aging:impaired and links adipose during alterations Proteasome JN. Keller and LI Szweda AJ, P, SO, Ebenezer Bruce-Keller L, Zhang K, Dasuri Fernandez-Kim regulation. and TDP-43 PENNINGTON RESEARCH CENTER BIOMEDICAL PLoS One PLoS 2011; 53(6):412-8. 2011; 6(5):e19657. 2011; 84(2):275-9. 2011; 6(8):e23394. J Neurosci Res Neurosci J Fundam Clin Pharmacol J Am Diet Assoc Diet J Am Int J Behav Med JBehav Int Free Radic Biol Med Chem Res Toxicol Res Chem 2011; 89(9):1471-7. 2011; Sleep Diabetes 2011; 34(10):1291-7. 2011; Appl Physiol Nutr Metab 2011; 111(12):1826-35. 2011; 2011; 2011; Exerc Sci Sports Med 2011; 18(3):188-98. 2011; 2011; 60(5):1380-2. N Engl JMed N Engl 2011; 51(9):1727-35. 2011; 24(2):204-16. 2011; 2011; 25(2):211-6. 2011; Tissue Eng Part A Eng Part Tissue 2011; 2011; Prog Prog

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2011; 2010:604713. 2011; 2011; 7:67-73. 2011; J Gerontol A Biol Med Sci Sci 2011; 29(2):97-105. 2011; 14(6):812-7. 2011; Stem Cells Int Cells Stem 2011; 377(9774):1341-52. 2011; 2011; 35(9):1241-6. 2011; 2011; 20(8):1295-6. 2011; Lancet SCIENTIFIC REPORT SCIENTIFIC 2011; 30(4):290-300. 2011; 2011; 29(5):749-54. 2011; 3:69-81. 2011; Pharmacoeconomics 2011; 21(16):R624-6. 2011; Int J Obes (Lond) Stem Cells Dev Cells Stem Matrix Biol Matrix Stem Cells Stem Curr Biol Curr Clin Pract Epidemiol Ment Health Ment Epidemiol Pract Clin 2011; 286(25):22227-34. 2011; 2011; 18(8):579-86. 2011; 2011; 9(1):1-2. 2011; 2011; 26(8):1694-7. 2011; 2011; 66(11):1211-7. Hornbrook Bauck A, Brantley KL,Funk VJ, M, PJ, Jerome Stevens GJ, Myers VH and Appel L. Development andImplementation of a Tailored Self-assessment in an Internet-based Tool Loss Maintenance Weight Program. Gadde KM,Allison DB, Ryan DH, Peterson CA, B, Schwiers Troupin ML and Effects WW. Day of low-dose,controlled-release, phentermine comorbidities associated and weight on combination topiramate plus in overweight and obese a randomised, adults (CONQUER): placebo- controlled, phase 3 trial. Galgani JE, Smith and SR Ravussin E. Assessment of EchoMRI-AH versus dual-energy absorptiometry X-ray measure to human body composition. Gandjour A. Prioritizing comparative effectiveness research: are drug implementationand equally trials worth funding? 29(7):555-61.2011; Gandjour A. model A transfer to trial-based pharmacoeconomic analyses practice. clinical to Gimble JM. balancing Leptin’s act between bone and fat. Res Gimble JM, Bunnell BA, Casteilla L, K. Jung and JS Yoshimura Phases I-III Cells. Adult Using Stem Clinical Trials Gimble JM, Concise Bunnell review: BA, Adipose- Chiu ES and Guilak F. derived stromal vascular fraction and cells stem let’s cells: not get lost in translation. Gimble stem beyond JM, cells Taking Bunnell BA, Chiu ES and Guilak F. discovery: milestone reporting a the in regulatory of requirements for cell therapy. Biol Chem Gawronska-Kozak B. Scarless skin deficient wound healing in FOXN1 associated mice is with(nude) distinctive matrix metalloproteinase expression. Gimble JM, Bunnell and ZE. BA Floyd Prospecting for adipose progenitor cell biomarkers: biopanning for gold with in vivo phage display. LopezGimble MJ and JM, Vunjak-Novakovic G. Guilak Grayson F, W, Adipose tissue as a stem cell source for musculoskeletal regeneration. Biosci Ed) Front (Schol Cell Gimble JM and ZE. Floyd Metabolism: what causes the gut’s circadian instincts? Flanagan M, Gimble G, S and X, Li S. JM, Wu Xia X, Yu Yao Hu J, therapy. cell for electroporation formulation Competitive Ther Perreault L, Marcovina SM, BrayGA, Crandall Saudek JP, Florez H, Ma Y, Barrett-ConnorCD, E and Knowler WC. Parental longevity and diabetes risk in the Diabetes Prevention Program. Gandjour A, Holler A, Dipl Ges O and Adarkwah Cost-effectiveness CC. of implantable defibrillators aftermyocardial infarction based on 8-year follow-up Health data Value (MADIT II). Gao Z, Zhang Kheterpal J, I, Kennedy Sirtuin J. N, RJ Davis 1 and Ye protein degradation in N-terminal response(SIRT1) persistent to c-Jun activationkinase contributes 1 (JNK1) hepatic to steatosis in obesity.

2011; Int J 2011; PLoS One J Nutrigenet Nutrigenet J 2011; 102(4):448-52. 2011; 2011; 12(5):e438-53. 2011; 2011; 32(11):882-8. Scand J Med Sports Sci Obes Rev J Gerontol A Biol Med Sci Sci Mol Genet Metab Genet Mol 2011; 28(4):629-35. Int J Mol Med 2011; Int J Sports Med J Int Lancet Infect Dis 2011; 11(12):963-9. 2011; 8:6. 2011; 35(11):1363-76. 2011; 152(10):3648-60. 2011; 2011; 4(3):137-45. 2011; 2011; 28(3):187-203. 2011; J Inflamm(Lond) 2011; 2011:820101. 2011; Int J Obes (Lond) Endocrinology

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PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Hypertens Nutrigenomics Dolley G, Boisclair Bouchard ME, Lamarche C, Perusse B, Despres JP, MC.L and Vohl Interactions between dietary fat intake and FASN geneticLDL variation influence peak particle diameter. Dolley Bouchard G, Lamarche C, Perusse MC. L and B, Despres Vohl JP, QTL linked LDL to peak particleInvestigation of LRP8 gene in 1p31 diameter in the Quebec family study. Dixit VD, Yang H, SayeedDixit KS, Yang KS, VD, Stote Rumpler Longo Baer WV, DJ, DL, Mattson Controlled DD. meal frequency MP and Taub without caloric restrictionalters peripheral blood mononuclear cell cytokine production. Dodani S and S, Pankey Sullivan Champagne D, C. HEALS: a faith-based hypertension control and prevention program for African American churches: training of church leaders as program interventionists. Dhurandhar NV. A framework A Dhurandhar NV. for identification of infections that contribute human to obesity. Doring F, Onur S, KurbitzDoring C, Boulay F, MR, Perusse Rauramaa L, Rankinen T, R, Wolfarth B and Bouchard C. Single nucleotide polymorphisms in the myostatin (MSTN) and muscle creatine kinase genes (CKM) are not associated with endurance elite performance. 21(6):841-45. 2011; Zhang Kuchmerovska J, Stavniichuk R,W, TM, Xu Drel VR, Pacher P, Slusher B and Obrosova IG. Poly(ADP-ribose)polymerase inhibition counteracts hypertrophy renal multiple and manifestations peripheral of neuropathy in diabetic Akita mice. Dubuisson Dhurandhar O, EJ, Krishnapuram R, Kirk-Ballard H, Gupta AK, E, Gimble Floyd Hegde JM and PPARgamma- Dhurandhar V, NV. independent adiponectinand uptake glucose increase in in abundance fat cells. Durgan DJ, Tsai JY, Grenett Villegas-Montoya JY, MH, BM, Pat Ratcliffe Tsai Durgan C, WF, DJ, Garvey ME, Nagendran Dyck J, JR, Bray MS, Gamble KL, Gimble JM and ME. suggesting cardiomyocyte Evidence the that Young circadian clock modulates responsiveness of the heart hypertrophic to stimuli in mice. Int Chronobiol Blair and SN Church TS. DoesEarnest age attenuate aerobic CP, Eur J Appl Response. women? postmenopausal in response conditioning Physiol Lupo M, White KM andEarnest Church TS. Effect CP, of astaxanthin on cycling time trial performance. MS, ButrynFaith Fabricatore A, M, Nguyen Wadden TA, AM and depression among associations prospectivefor Evidence SB. Heymsfield and obesity in population-based studies. Elks CM, Reed Mariappan SD, N, Shukitt-Hale B, Joseph JA, Ingram DK and A blueberry-enriched Francis J. diet attenuates nephropathy in a rat model of hypertension via reduction in oxidative stress. 6(9):e24028. Higginbotham Fabricatore AN, Wadden TA, AJ, Faulconbridge LF, Nguyen AM, HeymsfieldFaith MS. SB andIntentional weight loss and changes in symptoms of depression: a systematic review and meta- analysis. Fielding RA, Espeland Blair Rejeski S, MA, Church T, Gill WJ, TM, Guralnik JM, FC, Hsu Katula King J, Kritchevsky AC, SB, McDermott MM, Miller ME, Nayfield S, Newman AB, Williamson D, RomashkanBonds JD, S, Hadley E and Pahor M. The Lifestyle Interventions and Independence for Elders Study: design and methods. 66(11):1226-37. Journal ArticlesJournal 2011 112

PUBLICATIONS 2011 Journal Articles health. health. of Survey Canada/U.S. joint the sedentarism: of Tudor-Locke Markers C. and A Bauman RE, Andersen C, Cameron JM, Griffiths CL, Craig TL, Hart activity in 10-11-year-oldactivity children. physical assessing for instruments objective low-cost, of Evaluation T, Tudor-Locke Brusseau JJ and TL, C. KulinnaMcClain PH, Hart Exerc activity. physical and behavior sedentary of measures subjective and Tudor-Locke and Objective BE C. Ainsworth TL, Hart colitis. chronic of model amouse in tissue and inflammation hypoxia between Relationship MB. Grisham and M Kosloski-Davidson S, Zhang MN, Yadav PR, Watts AS, Carter NR, Harris Sci Sports logger. activity ActivPAL physical the using measurement step and estimates MET of Validation AE. Donnelly and Welk GJ DM, Harrington Appl Physiol Nutr Metab Delta. Mississippi Lower the in guidelines activity physical of translation PT. Step-based Katzmarzyk WD, Rand Allen Johnson DW, BM, Kennedy ST, Broyles Harsha CM, Tudor-Locke Champagne DM, C, Harrington adolescent females. in time sedentary measured objectively of patterns and levels of analysis KP, Dowd Cross-Sectional AE. DM, Donnelly Harrington AKand Bourke 2011;2010. Exerc Sci Sports 43(5):743-52. Med in genomics performance and fitness, exercise, in Advances C. Bouchard Band Wolfarth SM, Roth L, T, Perusse RJ, Rankinen Loos JM, Hagberg events. cardiovascular accelerated apathwayfor adults: healthy in prediabetes and prehypertension WD. Coexisting Johnson and MM Brashear AK, Gupta levels. Diabetes Care D vitamin low with associated are adults healthy in prehypertension and WD. Prediabetes Johnson and MM Brashear AK, Gupta treatment. 2011; Coll Nutr JAm 30(3):178-81. biotin Tomayko Treadwell Kand to high-dose BV. responds taste of Loss Cox L, SR, Smith M, Hausmann E, Ravussin DK, Ingram FL, Greenway treat hypertension. to extract bark Oliver ulmoides Eucommia standardized ofa efficacy and safety the F, testing Greenway trial Aclinical YuA. Z, Liu Gupta Yand interaction. drug F,Greenway Yu Kand apotential Fujioka Y. multivitamins: Vomiting from morbidly cohort. obese a from expression gene liver, stomach, adipose of and genetics the of Asurvey PY, Lum LM. ML, Kaplan EE and Reitman DM, Kemp SB, Schadt Heymsfield C, Wang Molony S, SK, V, Castro B, J, Zhang ZhuJ, Sieberts Beaulaurier C, Suver IJ, Hatoum E, Chudin R, Dobrin DM, Greenawalt 14(6):554-61. 2011; tissue: in adipose anrhythms update. Circadian BA. Floyd Bunnell ZE and AA, Ptitsyn GM, Sutton JM, Gimble Endocrinol tissue metabolism. inadipose and influencesof clocks circadian Prospective Floyd ZE. and AA Ptitsyn BA, Bunnell GM, Sutton JM, Gimble 17(4):332-9. 2011; applications. pharmaceutical medicine: in regenerative cells (ASC) stromal/stem Adipose-derived ME. Nuttall and JM Gimble SCIENTIFIC REPORT 2011; 43(3):449-56. 2011; J Phys Act Health J Phys Act 2011; 29(6):627-33. 2011; 7(2):98-107. 2011; 2011; JTher Am 18(6):453-7. Altern Med Rev Med Altern 2011; 34(3):658-60. Int J Behav Nutr Phys Nutr Act JBehav Int Hypertens Res Hypertens 2011; 36(4):583-5. 2011; 8(3):361-71. 2011; Genome Res

2 2011; 16(4):338-47. Res Q Exerc Sport QExerc Res 010–2011 Inflamm Bowel Dis Bowel Inflamm 2011; 21(7):1008-16. 2011; 2011; 34(4):456-61. Curr Opin Clin Nutr Metab Care Metab Nutr Clin Curr Opin 2011; 8:120. 2011; 82(4):600-9. 2011; 2011; 17(3):742-6. 2011; Med Sci Sports Sci Sports Med Curr Des Pharm Nat Rev Rev Nat J

Clin Nutr composition to height: relevance to height-normalized indexes. body of Scaling A. Pietrobelli and D Thomas M, Heo SB, Heymsfield circumferential indexes. and ranges to normative height? Relevance with associated circumferences body adult Are A. Pietrobelli and M Heo SB, Heymsfield Obes Facts factors. lifestyle and socioeconomic demographic, for controlling after even BMI and intake vegetable and fruit between association Inverse and SB MS. Faith Heymsfield DB, J, Allison Wylie-Rosett RS, Kim M, Heo Metab 2diabetes. type with patients in secretion insulin and stimulation beta-cell on RY,saxagliptin JF.of List and RS Chen Effects CF, Deacon SR, JJ, Mudaliar Duan SL, Holst Schwartz SR, Smith RR, Henry 19(12):2351-60. obesity. for pharmacotherapy phentermine long-term during maintenance and loss weight effects, rate heart and pressure Blood AK. Gupta EC and Westman FL, Greenway EJ, Hendricks program. management aweight in patients in cessation phentermine abrupt of Astudy FL. Greenway and EJ Hendricks 2011; 6(5):e19907. Nutrition Examination Survey Epidemiologic Follow-up Study. and Health National First mortality--the and damage skin Actinic S. Zhu and SB W,He Heymsfield ZhuF, Z, Chen M, Zheng X, Zhao X, Ma Metab JPhysiol Endocrinol Am hypoxia. and insulin, adipogenesis, factors: tissue in by adipose obesity-associated activity J, J, Yun Zhang Yin Z, Ye Zand Gao HIF-1{alpha} of Q, He J. Regulation Health Phys Act behaviors. active and sedentary of measures objective of evaluation free-living and Tudor-Locke JJ and Controlled C. McClain TL, Hart contribution of abdominal obesity. pressure: blood exercise increased and fitness, cardiorespiratory low resistance, Insulin C. Rheaume and JP Despres C, Bouchard A, V, Gaudreault BJ, P, Poirier Arsenault M, Huot Tremblay L, Perusse (Lond) Obes and race/ethnic changes mass index: free inadulthood. differences Gallagher D. and TB Fat- Vanitallie JR, J, Fernandez Albu S, Heymsfield Pi-Sunyer Z, X, Jr., RN, Kaleem J, Wang J, Pierson Thornton HR, Hull Alcohol model. abuse alcohol chronic arat cells in stromal adult of multipotentiality and expansion Impaired MJ. Lopez and S Nelson GJ, Bagby JM, ND, Gimble Spencer NK, Huff adults. adults. American African and white in factors risk cardiometabolic and adiposity PT. Trunk extremity versus Katzmarzyk and DH Jr., Ryan E, Ravussin RL, WD, Newton Johnson FL, Greenway GA, Bray C, Bouchard G, Hu Peptides BBB. across the leptinonleptin receptortransport mutation specific of cell-type Pan W. and IJ Effects Magrisso SS,S, Jr., Chua SB, Obici Stone KP, EN, Tu AJ, Markadakis H, Kastin H, Hsuchou Wang Y, Heymsfield 12(5):e348-61. 2011; changes. composition body phase early of review systematic loss: weight Voluntary MJ. Muller Aand Bosy-Westphal B, Strauss Shen C, W, Martin Peng JZ, AM, Nguyen Thomas D, SB, Heymsfield 94(6):1650-1. 2011; recognized. generally than larger are digestibility energy apparent in differences Individual A. Pietrobelli and SB Heymsfield 2011; 13(9):850-8. 2011; PENNINGTON RESEARCH CENTER BIOMEDICAL Diabetes Care 2011; 32(7):1392-9. 2011; 2011; 93(4):736-40. 2011; 4(6):449-55. 2011; 35(1):121-7. 2011; 2011; 8(6):848-57. 2011; 34(6):1415-8. 2011; 2011; 93(2):302-7. 2011; Nutr JClin Am Hypertension 2011; 45(4):393-402. Obesity (SilverObesity Spring) 2011; JTher Am 18(4):292-9. 2011; 58(6):1036-42. 2011; 300(5):E877-85. Diabetes Obes Obes Diabetes Nutr JClin Am Obes Rev Obes PLoS One PLoS Am J Am 2011; 2011; Int J Int J

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Am J 2011; Menopause 010–2011 J Proteome Res Proteome J 2 PLoS Med Lab Anim 2011;

2011; 34(11):2448-53. 2011; 278(1712):1626-32. 2011; 2011; 36(4):570-6. 2011; 2011; 1367:207-12. Diabetes Care Diabetes Proc Biol Sci 2011; 25(8):1789-95. 2011; in Vitro Toxicol Brain Res Brain SCIENTIFIC REPORT SCIENTIFIC 2011; 300(5):E779-89. 2011; and Bray GA. Olfactory bulbectomy impairs the feeding response to 2-deoxy-D-glucose in rats. Klimentidis Murati Beasley G, YC, GlassTM, Lin GE, HY, Guyton M, Newton Jorgensen W, M, HeymsfieldKemnitzSB,Fairbanks J, L and Allison DB. Canaries in the coal mine: a cross-species analysis of the obesityplurality of epidemics. Kosmiski LA, Scherzer R, HeymsfieldSB, Simberkoff D, Rimland MS, Sidney Biggs S, Shlipak ML and MG, Grunfeld Bacchetti C. Association P, of increased upper trunk and decreased leg fat with 2-h glucose in control and HIV-infected persons. Krishnapuram R, Dhurandhar EJ, Dubuisson Kirk-Ballard O, H, Bajpeyi S, Butte N, Sothern MS, Larsen-Meyer E, Chalew S, Bennett B, Gupta AK, Greenway Brashear FL, Johnson M, W, Reinhart G, Bouchard Rankinen T, Javier J, R, to ZuberiC, Cefalu Ye A and Template Dhurandhar WT, NV. without control reducing glycemic adiposityimprove or dietary fat. Metab Endocrinol Physiol Krishnapuram R, Kirk-Ballard H, Zuberi A and Infectivity Dhurandhar NV. period of mice inoculated with human adenoviruses. 45(2):103-8. JL,Kuk Ardern CI, Church TS, Sharma AM, Padwal R, Sui X and Blair SN. Edmonton Obesity Staging association System: with weight history and mortality Metab Nutr risk. Physiol Appl Kheterpal G, I, G, Ku Coleman Ptitsyn L, Yu AA, and ZE Gimble Floyd JM. Proteome of human subcutaneous adipose tissue stromal vascular fraction versus cells mature adipocytes based onDIGE. 2011; 10(4):1519-27. Kilpelainen Qi L, Brage TO, S, Sharp SJ, Sonestedt E, Demerath E, Mora S, KaakinenAhmad T, M, Sandholt CH, Holzapfel C, Autenrieth CS, Hypponen E, Cauchi S, He M, Kutalik Z, Kumari M, Stancakova A, Meidtner Mangino M, K, Timpson JT, Zillikens Balkau NJ, Song B, Tan Y, MC, Jablonski KA, Garcia ME, Johansson S, Bragg-Gresham JL, Y, Wu Onland-Moret Vliet-Ostaptchoukvan JV, NC, Zimmermann Rivera E, Stringham HM, Silbernagel T, G, Tanaka Kanoni S, FeitosaNV, MF, Snitker S, Ruiz JR, Metter M, Atalay Hakanen Larrad J, M, MT, Amin N, Cavalcanti-Proenca C, Grontved A, Hallmans G, Jansson Kuusisto JO, KahonenJ, M, Lutsey PL, Nolan L, Palla Pedersen JJ, Perusse O, L, Scott RA, TH,Renstrom Ronnemaa Sovio Tammelin Shungin F, U, D, Lakka Uusitupa M, TA, T, Rios MS, Ferrucci L, Bouchard C, Meirhaeghe A, M, M, Fu Walker Borecki Fritsche IB, Dedoussis A, GV, Ohlsson C, Boehnke M, Bandinelli S, van Duijn CM, Ebrahim S, Lawlor DA, HarrisGudnason TB, Sorensen V, TI, Mohlke KL, Hofman A, Uitterlinden Raitakari Lehtimaki J, Isomaa O, B, Njolstad T, Tuomilehto AG, PR, BoeingFlorez JC, H, Laakso Liu S, Ness A, ES, Spector Froguel P, Tai TD, M, Marmot M, Bergmann Ridker C, S, Chasman P, Power Khaw D, KT, Monda Jarvelin KL,Hansen MR, Illig T, T, NJ, Groop Wareham Hu FB, Orho-MelanderLC, M, Franks Ekelund U, PW and Loos RJ. Physical activity attenuates the influence of variantsFTO on obesity risk:a children. adults and 19,268 meta-analysis of 218,166 8(11):e1001116. Kim AR, Lee MS, Shin TS, Hua H, Jang BC, Choi JS, Byun DS, Utsuki T, Ingram Kim and D HR. Phlorofucofuroeckol inhibits A LPS-stimulated the expressions in macrophagesiNOS and via COX-2 inhibition of NF- kappaB, Akt, MAPK. and p38 Dabelea KitabchiKim D, C, Edelstein AE, Hamman SL, RF, Crandall JP, Montez MG, Perreault L, Foulkes MA and Barrett-Connor E. Menopause and risk of diabetes in the Diabetes Prevention Program. 18(8):857-68.2011; King BM, Primeaux Zadeh SD, ML, de Gruiter Ferguson JE, JD, Plant AV

Am J 2011; Curr SportCurr 2011; Free RadicFree Biol 2011; Diabetes 2011; 25(5):1473-80. 2011; Biochem Soc Trans PLoS One 2011; 19(6):1272-8. 2011; Health Educ Res Oncol Rep Oncol 2011; 46(2-3):148-54. 2011; Exp Gerontol 2011; 43(10):1913-9. Obesity (Silver Spring) Obesity (Silver 2011:1-7. 2011; 31(1):41-9. 2011; 124(10):931-8. 2011; 2011; 18(3):90-4. Br J Nutr Am J Med J Appl 2011; Physiol 110(1):46-59. Reprod Toxicol Reprod 2011; 40(5):566-71. 2011; 10(4):217-23. 2011; Med Sports Sci Exerc

2011; 51(9):1636-42.

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Kennedy BM, Bookman Harsha DW, EB, Hill YR, Rodarte Rankinen T, RQ and Challenges Murla CD. recruitment to and retention of African Americans in the gene-environment trial of response dietary to interventions (GET READI) for heart health. 2011; 39(3):833-7. 2011; Katzmarzyk Cost-Effectiveness PT. of Exercise Is Medicine (R). Rep Med Katzmarzyk Bray GA, Greenway PT, FL, Johnson Newton WD, RL, Jr., Ravussin E, Ryan DH and Bouchard C. Ethnic-specific BMI and waist thresholds.circumference Katzmarzyk Champagne Harsha C, CM, Broyles PT, Tudor-Locke ST, KennedyD, BM and Johnson A short-term WD. physical activity randomized trial in the Lower Mississippi Delta. 6(10):e26667. NB, Vollaard Gustafsson Gallagher IJ, Sundberg T, CJ,Keller Rankinen P, Britton SL,T, Bouchard and C, Timmons Koch LG JA. A transcriptional map of the impact of endurance exercise training on skeletal muscle phenotype. Inoue S, Ohya Y, Odagiri Y, Takamiya T, Suijo K, Kamada T, M, Okada Takamiya Odagiri S, Inoue Y, S,Ohya Y, Sociodemographic C and ShimomitsuTudor-Locke T. determinants of activity adults. physical Japanese among pedometer-determined Prev Med N andShimomitsu S, Yoshiike C, Tanaka Tudor-Locke Inoue S,Ohya Y, Time trends forT. step-determined physical activity among Japanese adults. Jackson AS, JanssenI, Sui X, Church TS and Blair SN. Longitudinal changes in body composition associated with healthy ageing: men, aged 20-96 years. Kirk-BallardEilertsenD, H, Wolff L, Liu Zhang F, K Koh GY, Jeansonne DP, Z. Liu and Paclitaxel-induced blocked camptothecin is in by apoptosis human breast and pancreatic cancer cells. Ilnytska O, Stutz AM, Park-York M, York DA, RibnickyIlnytska M, York DM, Stutz O, Zuberi AM, A, Park-York Cefalu WT and Argyropoulos G. Molecular mechanisms for activation of agouti-relatedthe appetite. stimulation of and protein 60(1):97-106. Ingram DK and Roth GS. Glycolytic inhibition as a strategy for developing calorie restriction mimetics. Johnson Brashear WD, MM, Gupta AK, Rood JC and Ryan DH. cardiometabolic improves loss Incremental weight extremely in risk obese adults. Kadiiska MB, Hensley GE, Hatch K, Nyska R, Stocker A, George Jones DP, MM, Thiel Van DH, Stadler K, Barrett Biomarkers JC and Mason RP. of Oxidative Stress ozone Study exposure IV: of rats and its effect on antioxidants in plasma and bronchoalveolar lavage fluid. Med Kappen C, Kruger C, MacGowan J and Salbaum JM. Maternal diet modulates the risk for neuraltube defects in a mouse model of diabetic pregnancy. Kashfi K, Mynatt RL,Park EA Cookand GA. Membrane microenvironment regulation of carnitine palmitoyltranferases I and II. 26(5):923-36. Kennedy BM, Prewitt TE, McCabe-Sellers B, Strickland K, E, Yadrick Champagne CM, McGeeThreadgill BB and P, Bogle ML. Academic partnerships and leaders key emerging from communities in the lower Mississippi Delta a community-based (LMD): participatory research Divers J Cult model. Journal ArticlesJournal 2011 114

PUBLICATIONS 2011 Journal Articles inflammation. inflammation. of markers on training exercise and fitness, cardiorespiratory activity, CP. Earnest and RV physical of Milani TS, Church Impact CJ, Lavie Diabetologia children. lipids of are insulin determinants in resistance prepubertal intramyocellular and Intrahepatic M. Sothern and WT Cefalu S, Chalew B, Volaufova J, E, Bennett Ravussin BR, Newcomer DE, Larson-Meyer variability. pressure blood circadian abnormal induces Dysglycemia AK. Gupta Band Joe WD, Johnson NG, Abraham DH, Kim K, Kumarasamy Gopalakrishnan S, regulation of dopaminergic neurotoxicity. ofregulation dopaminergic microglia: neuroinflammation, and air and primes pollution, activates exhaust P, Kodavanti L, Diesel Duke JA, ML. Johnson Block and MJ Surace T, A, Wagner Taetzsch S, K, Levesque U, Kodavanti LullME, Stadler women. and men in mortality all-cause of predictors as fitness cardiorespiratory and activity physical leisure-time of Comparisons SN. Blair and PT Katzmarzyk U, Ekelund RA, Winett TS, YS, Church FB, Kim Ortega X, Sui DC, Lee Study.Longitudinal Center Aerobics the men: in mortality disease cardiovascular and all-cause on index mass body and fitness cardiorespiratory in changes of effects HW,Long-term Kohl SN. FC, Blair 3rd and PA, Stanford McAuley TS, Church IM, Lee EG, Artero X, Sui DC, Lee Metab Endocrinol Clin of in the changes rhythms. leptin circadian independently restriction by caloric induced adaptation metabolic the of determinant is amajor concentration leptin in fall The V, LM. Lecoultre Redman Eand Ravussin Care Metab Nutr Clin aging. and tissue adipose Brown E. Ravussin Vand Lecoultre 2011; Dis Infect 204(8):1246-55. wasting. SAIDS-associated to alcohol-accentuated may contribute processes catabolic and anabolic Disrupted PE. Molina and WT Cefalu JP, Wang NJ, S, ZQ, Dufour Nelson GJ, LeCapitaine Bagby BJ, Potter and thermogenic capacity during aging. aging. during capacity and thermogenic phenotypes metabolic differentialghrelin and ghrelin receptor exhibit Y. Sun and VD of Dixit Ablations M, Fiorotto Lu X, G, Qin L, Lin X, Ma differenceeffects. sex injuries: crash vehicle motor non-fatal and Obesity ZhuS. and DB PW,Allison SB, F, Laud Pintar W, X, Ma Shen A, Heymsfield Shih JE, Kim function. nerve peripheral accumulation: correlation with adduct tissueneural 4-hydroxynonenal oxidative and stress systemic alleviatesinhibition diabetes-induced PARP IG. Y, Obrosova and VR Maksimchyk H, Drel Shevalye S, Lupachyk 12(62):65-74. 2011; diseases and autoimmunity. inflammatory obesity-related in activation TD. Inflammasome Kanneganti and VD Dixit Lukens JR, Int subjects. white and black, Chinese, among density mineral bone with fat trunk percent and fat body percent of Relationships Zhu S. and SB W, Wu He Heymsfield Z, X, DB, FuMa Lu H, Wang Allison X, Z, 94(2):262-8. to 2009. 2000 from Louisiana in residents income low or middle in diabetes of prevalence Increasing G. Hu and DH J, Ryan J, Johnson Besse W,Li K, Xiao WangR, Y, Horswell L, Chen 119(8):1149-55. 2011; 22(12):3029-35. 2011; SCIENTIFIC REPORT 2011; 54(4):869-75. J Cardiopulm Rehabil Prev Int J Obes (Lond) JObes Int Cardiovasc Diabetol Cardiovasc 2011; 14(1):1-6. 2011; Circulation Br J Med Sports 2011; 96(9):E1512-6. Free Radic Biol Med

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2 2011; 35(9):1216-24. 2011; 2011; 45(6):504-10. 2011; 010–2011 2011; 10:104. 2011; 31(3):137-45. PLoS One PLoS 2011; 2011; Pract Clin Res Diabetes Environ Health Perspect Health Environ 2011; 50(10):1400-9. 2011; 2011; 6(1):e16391. Discov Med Discov Osteoporos Osteoporos Curr Opin Curr Opin 2011; 2011; J J

Technol soldiers. Reserve Army in data fitness physical longitudinal and (H.E.A.L.T.H.) program management weight Internet-based apilot of Efficacy DA. Williamson and DH Ryan TM, Jr., Stewart H, RL, Han Newton adolescents. rural in activity in differences ethnic measured Accelerometry DA. Williamson and LS Webber CK, Martin M, Sothern H, Han RL, Newton adults. African-American and in Caucasian risk factors cardiometabolic adverse of correlates are depots PT. adiposity Abdominal Katzmarzyk Dand Ryan F, Greenway G, Bray WD, C, E, Ravussin Johnson Bouchard RL, Newton detailed view on allometric scaling. rate in humans--a metabolic organs with and theirassociation individual of mass on constitution of A. Effect Bosy-Westphal and SB Heymsfield CC, Gluer W, M, Later I, D, Heller Gehrke Langemann MJ, Muller women. and men overweight in study acalorie-restriction in feeding controlled long-term to adherence Dietary E. Ravussin Jand Howard M, Most EA, Moreira Popul Health system. Manag hospital public a in guideline treatment dependence tobacco service health public States United 2000 the of implementation and Awareness M. Butler Moody-Thomas S, Horswell R, Celestin MD, Dellinger AB, Kaiser M and Mand Kaiser AB, MD, Dellinger Celestin R, Horswell S, Moody-Thomas non-users. and users contraceptive oral in variables eating in fluctuations cycle menstrual and PJ. pathology Geiselman ALand disorder Eating Copeland McVay MA, brainstem. the in interaction neural-glial atypical an of evidence receptors: AMPA via tract solitary the of nucleus the in astrocytes activates stimulation Vagal afferent RC. Rogers and GE Hermann DH, McDougal Sport adolescents. and children Australian in steps determined F. cut-points BMI-referenced for recommended daily pedometer- Tudor-Locke B, Bull Cand J, Giles-Corti Rutherford GR, McCormack diet. low-fat and alow-carbohydrate during appetite and preferences, food cravings, food in GD. BV, Iii Change Foster Sand Miller B, Klein Stein Bailer R, C, JO, Brill Hill PJ, HR, D, Geiselman H, Han Rosenbaum Wyatt CK, Martin Metab Endocrinol Clin expenditure. energy intakeinfluencing by without energy decreasing weight body reduces agonist, receptor a5-HT(2C) Lorcaserin, E. Ravussin SR and Smith CM, Anderson M, J, Sanchez Zhang LM, Redman CK, Martin 110(4):956-63. Physiol 2011; JAppl trials. randomized three of results humans: nonobese of levels activity physical free-living the on restriction JP, calorie of Team and WE Effect Kraus CS. Weiss EP, MA, McCrory SB, Delany Racette L, Lindblad SK, Das CK, Martin 2011; 66(12):1376-83. (LIFE-P). pilot Elders for Independence and Interventions Lifestyle the in disability mobility major of predictors as BMI and strength Muscle M. Pahor ABand Newman JM, Guralnik TM, Gill RA, Fielding AP, TS, Church ME, Marsh Miller WJ, MA, Rejeski Espeland AbetaPP/PS1Dtg mice. ovariectomized in loss neuron attenuates 17alpha-estradiol PR. Mouton Rand Cabo de DK, Manaye KF, AC, Xu Ingram Drew S, G, Kalifa JS, Allard 2011; 82(2):162-7. 2011; PENNINGTON RESEARCH CENTER BIOMEDICAL Nutr Diabetes 2011; 5(5):1255-62. J Neurosci J Obesity (SilverObesity Spring) J Phys Act Health J Phys Act Nutr Clin Pract 2011; 1:e2. 2011; 31(39):14037-45. 2011; 96(3):837-45. Eat Behav J Alzheimers Dis J Alzheimers 2011; 8(2):287-95. 2011; 12(1):49-55. 2011; 26(3):309-15. 2011; 2011; 19(10):1963-70. 2011; PLoS One PLoS 2011; 23(4):629-39. 2011; J Gerontol A Biol Sci Med Sci Sci Med ABiol J Gerontol 2011; 14(2):79-85. 2011; 2011; 6(7):e22732. Res Q Exerc QExerc Res J Diabetes Sci J Diabetes

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2011; Ann Med 2011; J Clin Biochim Lung Cancer Lung 2011; 2011; 33(2):155- 010–2011 Antioxid Redox Redox Antioxid 2

Contemp Clin Trials Fertil Steril Fertil 2011; 13(6):517-22. 2011; Age (Dordr) J Clin Endocrinol Metab 2011; 5(4):368-72. 2011; 6(2):e16957. 2011; Diabetes Obes Metab Obes Diabetes SCIENTIFIC REPORT SCIENTIFIC 2011; 57(6):1094-100. 2011; 2011; 96(2):E312-21. 2011; 1822(4):557-63. 2011; Curr Cardiovasc Risk Rep 2011; 124(5):563-71. 2011; 14(2):275-87. Redman Guillot LM, TS and Greenway Moro FL. C, Y, Dobak Yu J, Association adrenergic agonist corticosteroid and beta-2 of injection in the treatment of lipomas. Redman LM and Ravussin E. Caloric restriction in humans: impact on outcomes. behavioral and psychological, physiological, Signal Reka AK, Krishnapuram Goswami R, MT, and Keshamouni Standiford TJ MolecularVG. cross-regulation between other and PPAR-gamma signaling pathways: implications for lung cancer therapy. 2011; 72(2):154-9. A, PerusseRheaume Bouchard L, Tremblay C, Arsenault C, BJ, Dumas MP, Contributions of cardiorespiratoryPoirier P and Despres JP. fitness and visceral adiposity six-year to changes in cardiometabolic risk markers in apparently healthy men and women. 96(5):1462-8. Richard AJ, Amini ZJ, Ribnicky DM and Stephens JM. Wort John’s St. adipocytes. murine human and in signaling inhibits insulin Acta Biophys Rickman Williamson AD, DA, Martin CK, Gilhooly RI, CH, Stein Bales CW, Roberts S and Das SK. The CALERIE Study: design and methods of an caloric restriction 25% innovative intervention. 32(6):874-81. PsychogiosGuo N, Peng J, Mandal Hau AC, DD, R, Bouatra S, Sinelnikov I, Krishnamurthy R, Eisner R, N, Gautam Xia Knox J, C, B, Young Dong Hollander Z, PedersenTL, E, Huang Smith P, Greiner SR, Bamforth R, F, McManus Goodfriend B, Newman JW, T and Wishart DS. The human PLoS metabolome. serum One Ptitsyn AA and Gimble or false: JM. all genes True are rhythmic. Qiu G, Wan R, Hu J, Mattson MP, Spangler E, Liu S, Yau SY, Lee TM, SpanglerQiu SY, G, R, Wan E, Hu Mattson Liu S, J, Yau MP, Gleichmann M, Ingram DK, So KF and Zou S. Adiponectin protects rat hippocampal neurons against excitotoxicity. 2011; 43(1):1-12. Qi Q, Bray GA,Smith SR, Sacks Hu FB, and Qi L. FM Insulin receptor substrate 1 gene variation modifies insulin resistance response weight-lossto diets randomized in a 2-year trial: the Preventing Overweight Novel Using Dietary Strategies (POUNDS LOST) trial. Circulation Qiu C, Hu G, Kivipelto M, Antikainen Laatikainen R, Fratiglioni T, L, Association J. Jousilahti P and Tuomilehto of blood pressure and hypertension with the risk of Parkinson disease: the National FINRISK Study. Hypertension 65. Rankinen T and Bouchard C. Genetic Predictors of Exercise Training Response. Ravussin E and Galgani JE. The implication of brown adipose tissue for humans. Annu Rev Nutr 2011; 31:33-47. Redman LM, Elkind-Hirsch K and Ravussin E. Aerobic exercise in women with polycystic ovary syndrome improves ovarian morphology body in composition. changes of independent 95(8):2696-9. Redman LM, Huffman KM, LandermanBain JR, LR, Pieper CF, Muehlbauer Wenner BR, RD, MJ, Stevens Kraus VB, Newgard CB, Kraus WE and Ravussin E. Effect of caloric restriction with and without exercise on metabolic intermediates in nonobese men and women. Metab Endocrinol

J 2011; 2011; 2011; Am J Clin Peptides 2011; 8(2):155-64. 2011; 152(4):1661-9. 2011; 6 Suppl 1:42-5. 2011; Curr Pharm DesCurr 2011; 43(3):478-84. 2011; 2011; 43(3):412-23. Am J Health Promot Clin Trials 2011; 12(3):373-85. 2011; 301(2):H555-64. 2011; Endocrinology J Mol Neurosci Mol J 2011; 96(5):E836-40. 2011; Int J Pediatr Obes J Mol Neurosci Mol J J Chromatogr B Analyt Biomed Technol Life Sci 2011; 2(6):49. 2011; Curr Pharm Biotechnol Pharm Curr J Clin Endocrinol Metab 2011; 226(3):843-51. 2011; Am Heart J Physiol Physiol Circ

Stem Cell Res Cell TherStem 2011; 94(3):809-18.

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Plaisance EP, Greenway FL, Boudreau A, Hill KL,Plaisance Johnson EP, Krajcik WD, RA, Perrone CE, Orentreich N, Cefalu WT and Gettys TW. Dietary methionine restriction obese increases in fat oxidation adults with metabolic syndrome. Pietrobelli A, Tosi C, Olivieri F, Lubrano Pietrobelli L and C, N. Fuiano Olivieri fatness From A, to Tosi F, leaness: where did go we wrong? Newton RL, Thomson JL, RauKK, Ragusa SA, Sample Singleton NN, AD, Webber LSAnton SD, and Williamson DA. Psychometric characteristics of process evaluation measures for rural a school-based childhood obesity prevention study: Louisiana Health. 25(6):417-21. Munzberg J, Nohara Waraich RS, Tong H Laque K, Zhang A, Y, Tiano JP, Early-lifeand Mauvais-Jarvis exposure F. testosterone to programs the hypothalamic melanocortin system. 2011; 879(9-10):627-32. Pieper C, Redman L, Racette S, Roberts S, Bhapkar M, Rochon Martin J, C, Das S, WilliamsonKraus W, D and Ravussin E. Development of adherence metrics caloric for restriction interventions. Primeaux QRFP in female SD. rats: effects on high fat food intake and hypothalamic gene expression across the estrous cycle. 32(6):1270-5. Pachon-Pena A, X, Bunnell Wu Vendrell J, G, and BA Gimble Tucker G, Yu JM. Stromal stem from cells adipose tissue and bone marrow of age- donors female distinctmatched display immunophenotypic profiles. Cell Physiol Padilla Whyte Simmons J, GH, Zderic JW, JJ, Davis TW, Hamilton MT, Bowles DK and Laughlin MH. Impact of exercise training on endothelial transcriptional profiles in healthy swine: a genome-wide microarray analysis. Hsuchou X, C, Wu Bouret H, Xu W, Pan SG and Kastin AJ. Astrocytes distribution the modulate in leptin of neuronal and signaling hypothalamus of obese A vy mice. Obrosova IG and Kador PF. AldoseObrosova reductase IG and Kador PF. / polyol inhibitors for diabetic retinopathy. Hsuchou H, Manda VK, Zhang Y and KP, Kastin Stone AJ. W, Pan Cytokine blood-brain modulates signaling barrier function. 17(33):3729-40. Khan Chatu F, X, Wu Kastin Hsuchou H, AJ, W, HalbergPan Zhang F, Y, RS, Robert B and Cornelissen-Guillaume GG. protective Potential role of during inflammation. IL15Ralpha Nutr Picou RA, Kheterpal Minnamreddy I, AD, Wellman M, G and Ku Gilman monomer Analysis of AbetaSD. and and Abeta fibrils (1-40) by (1-42) capillary electrophoresis. Pasiakos SM, McClung HL, McClung JP, Margolis SM,Pasiakos McClung LM, Andersen HL, McClung NE, JP, Cloutier GJ, Pikosky MA, Rood JC, Fielding AJ. RA and Leucine- Young enriched essential amino acid supplementation during moderate steady state exercise enhances postexercise muscle protein synthesis. Pandey AC, Semon JA, Kaushal D, O’Sullivan RP, GlowackiPandey Semon Gimble AC, J, O’Sullivan JA, Kaushal JM D, RP, and Bunnell BA. MicroRNA profiling reveals age-dependent differential expression of nuclear factor kappaB and mitogen-activated protein kinase in adipose and bone marrow-derived human mesenchymal stem cells. Journal ArticlesJournal 2011 116

PUBLICATIONS 2011 Journal Articles obese subjects study. subjects obese Swedish the in surgery bariatric after and before levels HDL-cholesterol with genes candidate GWAS-based of Association C. Bouchard and LM P, Jacobson Carlsson MA, L, T, Sjostrom B, Rankinen Carlsson Sarzynski cases. surgery bariatric SOS the in regain weight and loss weight maximal with genes 11 in candidate markers of obesity Associations LM. Carlsson C and P, Jacobson Bouchard MA, L, T, Sjostrom B, Rankinen Carlsson Sarzynski 17(12):1201-10. 2011; CMethods Eng Part conditions. xeno-free under system culture microcarrier-based a sources: human cells from stem mesenchymal of expansion grade Toward JM. aclinical- Cabral CL and Silva Vemuri S, MC, Boucher AM, LG, Campbell Chase JM, Gimble F,Santos MM, PZ, Abecasis Andrade pregnancy. pregnancy. in diabetes of model mouse a from placenta in differentiation spongiotrophoblast and expression gene Altered C. Kappen and DH Burk Pavlinkova G, NA, Delahaye X, Zhang C, Kruger JM, Salbaum epigenome? the for arole embryopathy: Diabetic C. Kappen and JM Salbaum 2011; 2:S178-87. 8Suppl activity. physical and visitation park on features and conditions park of role J. The Gustat and ST Broyles AJ, Mowen AL, Rung setting. apost-disaster in use park parks: neighbourhood in active being and to Escaping MS. Sothern Jand ST, Gustat Broyles AJ, AL, Mowen Rung Physiol Endocrinol Metab despite evident oxidative stress and mitochondrial ROS production. kidney the in bioenergetics mitochondrial of adaptation initial an induces diet High-fat K. Stadler Eand Cleland M, Ehrenshaft C, Ruggiero TX. Antonio, San from Americans Mexican population: minority U.S. growing arapidly 13 in of pathogens common Seroprevalence HH. Goring R and J, Yolken NV, Blangero B, Dhurandhar Grubbs E, CT, Leach Kraig R, Rubicz of common infections. infections. common of measures serological influence factors Genetic HH. Yolken Goring Rand J, Blangero NV, R, Dhurandhar Duggirala E, CT, Leach Kraig R, Rubicz imaging study.calcium Brain Res vitro in an nucleus: solitary the in hormone thyrotropin-releasing of action the amplifies Leptin GE. Hermann and DH McDougal RC, Rogers 66(1):97-108. Sci 2011; Med energy.of intake reducing of effects long-term the of assessment comprehensive study: CALERIE the of conduct and Design WE. Kraus EC and Hadley KM, Galan S, Romashkan SK, Das SB, Roberts SB, JO, Racette Holloszy LM, Redman CW, E, Ravussin J, Bales Rochon Stem disease. Krabbe’s Cells of model mouse twitcher the in effects anti-inflammatory pronounced cells have stem lineage Mesenchymal BA. Bunnell and JM Gimble SC, Li HP, Leonard ML, YT, Li McCants AF, X, Lin BA, Alvarez ME, Eagle S, Zhang Trygg Scruggs CB, JM, J, Fisher-Perkins Klopf-Eiermann Flaat CB, M, Ripoll 702:299-316. reprogramming using lentiviral-delivered shRNA. shRNA. using lentiviral-delivered reprogramming Screening for epigenetic target genes that KJ. enhanceEilertsen J, Power Rand Staszkiewicz L, Harkins C, Barnes K, Strickler JS, Rim in cycling. prolonged Beans Jelly to Sports alternative acost-effective are raisins Sun-dried LK. CP, CC Earnest Stewart TM, and Cortez Henagan BL, HL, Baker Rietschier BMC ResNotes SCIENTIFIC REPORT Disasters Diabetologia Birth Defects Res A Clin Mol Teratol Mol AClin Res Defects Birth Int J Obes (Lond) JObes Int 2011; 35(2):383-403. 2011; 2011; 4(1):433. 2011; J Strength Cond Res J Clin Endocrinol Metab Endocrinol Clin J Hum Hered 2011; 300(6):E1047-58. 2011; 54(7):1909-20. 2011; 29(1):67-77.

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2011; 1385:47-55. 2011; 72(2):133-41. 2011; 2 010–2011 2011; 25(11):3150-6. 2011; 2011; 96(6):E953-7. 2011; 91(8):770-80. Methods Mol Biol J Phys Act Health J Phys Act J Gerontol A Biol Sci ABiol J Gerontol Tissue Tissue 2011; 2011; Am J Am

and metabolic associations in HIV infection. infection. HIV in associations metabolic and tissue adipose P, Intermuscular C. Bacchetti Grunfeld and MG Shlipak DP, CE, Kotler Lewis SB, M, W, Shen R, Heymsfield Punyanitya Scherzer infection. HIV in mortality all-cause 5-year with associated are adiposity central increased and muscle limb P, Decreased C. Grunfeld and MG Shlipak Bacchetti PC, Tien Lee SB, WG, D, Powderly Heymsfield R, Scherzer technologies. proteomic gel-free and P, Gel-based I. Scherp Kheterpal Land Coleman Ku G, and bariatric surgery. surgery. bariatric and obesity by affected as functions reward Food HR. ACShin Berthoud and Study. Nutrition Jiangsu the from results adults: Chinese among follow-up a5-year in change weight and pattern Dietary G. Yuan Y, Dai Z, Shi G, Hu B, Holmboe-Ottesen ZuoHand Pediatr Obes tissue inof adipose quantification and inmuscle children. intervals Between-slice SB. Heymsfield Mand W,Shen J, Kwak Chen Punyanitya S, Res Food rat. Goto-Kakizaki in control glycemic maternal improves starch Dietary-resistant RJ. Martin Cand Williams A, Raggio MJ, Keenan L, Shen study. CARDIA the individuals: obese and overweight in resistance insulin with is associated mass fat PJ,Jr., extremity Lower S. Schreiner CE, Lewis Sidney Band Sternfeld DR, Jacobs C, Chan AL, Hankinson TR, Church MR, Carnethon Shay CM, 19(2):283-91. the CARDIA Fitness Study. Fitness CARDIA the follow-up: of 20 years after hypertension incident and genes candidate seven from interactions SNP-by-BMI and SNP-by-fitness C. Bouchard Sand Sidney T, M, Fornage B, Rankinen Sternfeld MA, Sarzynski impaired fat oxidation. fat impaired with is associated loss by weight phenotype subclass LDL dense small, of Siri-Tarino Reversal RM. PW, Krauss and AC,GA Woods Bray 51(1):120-30. (Trachemys elegans). turtle slider scripta in the red-eared iris photointrinsic the of sensitivity Spectral DH. McDougal CN and Casey BP, Jr., Selvarajah JR, Fink TE, JF, DA, Blaum GO, Dearworth Sipe Littlefield 66(1):29-37. cities. Indian five in hypertension and prehypertension for factors risk and Prevalence AK. Gupta and VV Muthusamy R, F, Meester de Agarwalo C, SK, Vajpeyee Sergey M, Tumbis Haque R, ZA, Begom K, Amit de S, J, Pella D, Fedacko Ghosh Z, RB, Macejova Singh 25(9):2488-95. athletes. judo elite in strength forearm maximal with distribution fluid and water total-body in changes between Relationship LB. Sardinha and SB Heymsfield DA, Fields AM, Silva rats. in reward food changes surgery bypass gastric Roux-en-Y HR. PJ Pistell H, AC,Shin Zheng Berthoud and Physiol Regul Integr Comp Physiol obesity,induced weight predisposition. leptin, loss, and genetic diet- high-fat by affected as stimuli food oily and sweet of “wanting” and “Liking” HR. Berthoud and LM AC,Shin Townsend Patterson RL, Med 2003-2007. from regions across children geographic school U.S. in behaviors Obesogenic K. Short Dand Brittain S, Broyles SB, Sisson 2011; 1:25-33. 2011; PENNINGTON RESEARCH CENTER BIOMEDICAL 2011; 55(10):1499-508. AIDS 2011; 6(2):149-56. 2011; 25(11):1405-14. 2011; Int J Obes (Lond) JObes Int Obesity (SilverObesity Spring) Methods Mol Biol J Hum Hypertens Obesity (SilverObesity Spring) 2011; 301(5):R1267-80. Int J Obes (Lond) JObes Int 2011; 353:S40-4. Suppl Br JNutr Br 2011; 702:163-90. 2011; 25(8):509-18. 2011; Obesity (SilverObesity Spring) 2011; 19(1):61-8. J Strength Cond Res 2011; 105(7):1047-54. 2011; 19(11):2248-53. 2011; 2011; 35(5):642-51. Acta Cardiol Acta Vision Res Vision Open JPrev Open Mol Nutr Nutr Mol Am J Am 2011; 2011; 2011; 2011; Int J Int 2011; 2011; 2011; 2011; PUBLICATIONS 117

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Cell Physiol Biochem Physiol Cell 2011; 8(3):445-53. 2011; Am J Hypertens Neurogastroenterol Motil Neurogastroenterol 2011; 39(3):235-41. 2011; 11(12):1677-85. J Clin Psychiatry 2011; 8(4):581-6. 2011; 2011; 8:80. 2011; Int J Pediatr Obes Endocrine J Phys ActJ Phys Health 2011; 53(3):178-81. 2011; SCIENTIFIC REPORT SCIENTIFIC J Phys ActJ Phys Health J Epidemiol Community Health Community Epidemiol J Prev Med 2011; 217(1):165-70. 2011; Expert Ther Opin Biol 2011; 5(6):579-99. 2011; Int J Behav Act Nutr Phys Behav Brain Res Brain Behav humans. J Biol Dyn M, Qualls-CreekmoreTong E, Browning RA KN, and Holmes Travagli GM. Experimental spinal cord injury in rats diminishes vagally-mediated gastric responses cholecystokinin-8s. to 23(2):e69-79. MH, GreerTrivedi TL, Church TS, Grannemann Carmody Galper BD, TJ, DI, Henley and SS Blair SN. Exercise Sunderajan as an P, Dunn AL,Earnest CP, a disorder: depressive nonremitted for major treatment augmentation comparison. dose parallel randomized, MH, GreerTrivedi TL, Grannemann Church TS, BD, Somoza E, Blair SN, Szapocznik Stoutenberg J, M, Rethorst Ring C, KM, Warden D, R, Walker Marcus B, Crowell B, Oden KosinskiMorris N and AS, T, Nunes Kyle DW, E. reduction Stimulant intervention using dosed exercise (STRIDE) - CTN study protocol0037: for a randomized controlled trial. 24(2):209-17. CS, BerthoudTam HR, Bueter M, Chakravarthy Geliebter A, MV, Hajnal A, Holst Kaplan J, Raybould L, Pories W, H, Seeley R, Strader A and Ravussin E. Could the mechanisms of bariatric surgery hold the for key novel reportSymposium.therapies? Scientific from Pennington a 2011; 12(11):984-94. CS, Heilbronn LK,Tam Henegar Cowley MJ, C, M, Wong CT, Cowell ClementKaplan K and Baur W, LA. An early inflammatory gene profile in children.visceral adipose in tissue CS, Lecoultre V andTam Ravussin E. Novel strategy for the use of leptin for obesity therapy. Thanos PK, Cho Kim J, R, Michaelides M, Primeaux S, Bray G, GJ Wang Bromocriptine ND. increased operant responding for high Volkow and fat food but decreased chow intake in both obesity-prone and resistant rats. Scribner R, Broyles Q,Theall Chotalia Simonsen KP, S, Yu J, N, Schonlau M Impact of small groupand size on Carlin neighbourhood BP. influences models. multilevel in Tudor-Locke C, BassettTudor-Locke DR, Shipe MF and McClain Pedometry JJ. methods for free-living assessing adults. Tudor-Locke C, AinsworthTudor-Locke BE, R. Washington TL and Assigning Troiano metabolic equivalent values the occupational to census 2002 system. classification Stone KP, Kastin AJ NFkB an unexpected is and W. Pan KP, Stone major mediator of cerebral endothelia. in signaling interleukin-15 28(1):115-24. J and Q, HeX, Yu Ye Liang F, Li M, H, Cai Xu M, Chen X, Bi Y, Zhu Y, Sun W, Inhibition J. Weng of obesity-induced hepatic stress ER early by insulin therapy in obese diabetic rats. Harris EL, Martin E, Ricchiuti WM, Vollmer Meltesen LP, GT, Svetkey Williams G, AppelV, LJ, Bray GA, Winn MP Moore and Conlin PR. TJ, Modulation of the BP response diet to genes by in the renin-angiotensin system and the adrenergic nervous system. Thomas DM, Martin CK, Heymsfield S, Redman LM, SchoellerDA and simple model A Levine JA. predicting change in individual weight Tudor-Locke C, Camhi SM,Tudor-Locke Leonardi C, Johnson Katzmarzyk WD, PT, Earnest and CP Church TS. Patterns of adult stepping cadence in the 2005-2006 NHANES. Tudor-Locke C, Craig CL, Aoyagi Y, Bell RC, C, Craig KA, Croteau CL,Tudor-Locke Aoyagi Y, De Bourdeaudhuij Matsudo Lutes LD, SM, Ramirez- HatanoI, Ewald Y, B, Gardner AW, Marrero Rogers MA FA, and Blair Rowe DA, LQ, Schmidt Tully MD, Forare olderSN. enough? How steps/day many adults and special populations.

PLoS J Phys J Phys 2011; Prev Med Obesity J Diabetes 2011; 19(6):1290- 2011; Osteoporos Int 2011; 116(4):544-53. J Gerontol A Biol Sci 2011; 18(2):280-90. 2011; 2011; 23(2):153-8. 2011; 60(7):941-9. 2011; J Neurochem J Obesity (Silver Spring) Obesity (Silver Curr MedCurr Chem Metabolism Aging Clin Exp Res Clin Aging 2011; 19(9):1731-4. 2011; 40(2):129-39. 2011; 230(1):106-13. 2011; 8:26. Exp Neurol 2011; 19(9):1796-803. 2011; 2011; 5(1):178-87. 2011; 2011; 8 Suppl 2:S188-97. 2011; Obesity (Silver Spring) Obesity (Silver

2011; 6(7):e21068. 2011;

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Sparks LM, Moro C, Ukropcova B, Bajpeyi S, Civitarese AE, Hulver MW, Thoresen GH, Rustan and Smith SR. AC Remodeling lipid metabolism primary human responsiveness in improvingand insulin myotubes. Act Health NewtonSisson SB, Broyles Baker ST, RL, BL and Jr., Chernausek TVs in SD. the bedrooms of children: does it impact health and behavior? 2011; 52(2):104-8. Smith BJ, Sutton G, GM, Goh X, Wu Yu BC, Hebert Pelled G, Gazit T, Z, Gazit Butler D, AA and Gimble JM. Ovariectomy and genes encoding circadian regulatorycore murine bone. in proteins 22(5):1633-9. Smith Mattison DL, Jr., JA, Desmond Kimura RA, M, Roth Gardner JP, GS, Ingram DK, Allison DB and A. Aviv dynamics Telomere in rhesus monkeys: no apparent effect of caloric restriction. Med 2011; Sci 66(11):1163-8. Smith SR, Stenlof KS, Greenway FL, McHutchison Schwartz J, SM, V BD, Berk ES and Kapikian R. Orlistat 60 mg reduces visceral adipose tissue: a 24-week randomized, placebo-controlled, multicenter trial. Spring) (Silver Schwarz JM,Smith TJ, Montain SJ, Rood Pikosky J, MA, Castaneda- Sceppa C, Glickman AJ. E and High Young protein diet maintains glucose production during exercise-induced energy deficit:controlled a trial. (Lond) Metab Nutr One Spencer Gimble ND, JM and Lopez MJ. Mesenchymal stromal past, cells: present, and future. Vet Surg Stadler K. Peroxynitrite-driven mechanisms in diabetes and insulin advances. latest the - resistance Sisson SB, Broyles ST, BakerSisson SB, BL Broyles and Katzmarzyk ST, reading, Television, PT. school-day of correlates time: computer and leisure-time sedentary behavior and relationship with overweight in children in the U.S. Stavniichuk R, Drel VR, Shevalye Kuchmerovska H, Maksimchyk TM, Y, Nadler JL and Obrosova IG. Baicalein alleviates diabetic peripheral inhibition through oxidative-nitrosative of neuropathy stress p38 and MAPK activation. Kastin Rapid AJ, endocytosis C and W. Hsuchou Pan H, Yu KP, Stone of cerebral by endothelia.interleukin-15 Stenholm S, Metter EJ, Roth GS, Ingram DK, DD Mattison JA, Taub Ferrucciand L. between Relationship leptin, insulin, ghrelin, plasma interleukin 6, adiponectin, testosterone longevity and Baltimore the in Aging. of Longitudinal Study Stephens BR, Granados K, Zderic TW, Hamilton MT and Braun B. Effects ofof inactivity 1 day on insulin action in healthy men and women: interaction with energy intake. 5. Stewart expression WC, Pearcy in LA, and ZE Stephens Floyd JM. STAT5A 3T3 promotes cells Swiss adipogenesis in vivo in an athymic mice model system. Stewart TM, Bachand AR, Han H, Ryan DH, Bray GA and Williamson DA. Body image changes associated with participation in an intensive lifestyle weight loss intervention. Stewart T, Han H, Allen RH,Stewart Bathalon T, G, Ryan DH, Newton and RL, Jr. Williamson DA. H.E.A.L.T.H.: efficacy ofinternet/population-based an behavioral weight management program for the U.S. Army. Sci Technol Journal ArticlesJournal 2011 118

PUBLICATIONS 2011 Journal Articles failure in Finnish men and women. women. and men Finnish in failure heart of risk the and consumption Coffee G. Hu and PT Katzmarzyk S, Wang Y, Tuomilehto P, Mannisto J, Jousilahti M, Mahonen R, Antikainen women. and men Finnish among failure to heart relation in factors Lifestyle G. Hu and PT Katzmarzyk Wang Y, Tuomilehto P, J, Jousilahti M, Mahonen R, Antikainen 108(30):12295-300. left-right asymmetry.phenotype-specific cells exhibit mammalian Micropatterned G. Vunjak-Novakovic Taylor M, Y, Park Zhang G, and K, JM Wan Gimble LQ, Ronaldson (SilverObesity Spring) success. long-term with associated factors study: AHEAD Look the in losses Four-year weight MZ. Vitolins and DH Ryan A, Otto J, Krakoff JO, Hill LM, Delahanty JM, Clark RR, Wing RH, TA,Wadden Neiberg 17(2):179-88. 2011; many steps/dayHow adults. enough? are for JC, Spence Teixeira GM, MD,PJ, Schofield SN. Schmidt Blair and MA Tully DA, Rowe JM, Oppert N, Mutrie Y, SM, Hatano B, Matsudo S, Inoue Giles-Corti K, Cocker WJ, De Brown SA, Tudor-Locke CL, Craig Clemes C, adolescents. and many steps/day How children SN. enough? are for Blair S and Tanaka JC, Spence DA, Rowe A, Raustorp TS, Y, Olds Hatano DR, Lubans S, Duncan GM, MW, Cardon S, Belton Beets Tudor-Locke CL, Craig C, instigates obesity-induced inflammation and insulin resistance. instigates obesity-induced inflammasome NLRP3 VD. The Dixit and JM Stephens E, Ravussin RL, Mynatt K, Stadler JE, Galgani Youm B, A, Vandanmagsar Ravussin YH, patients. diabetes 2 type for program modification abehavioral in change activity physical of Mediators I. Bourdeaudhuij De Nand Owen JM, J, Tudor-Locke Ruige B, Kaufman CE, Deforche K, Van D, Greef Dyck De in liposarcoma. activity suppressor reveals MicroRNA-143 characterization and functional sequencing tumor Tuschl RNA Small C, S. Singer Sander T and CR, Antonescu A, Crago A, Viale JM, Gimble Taylor R, A, Sheridan BS, Mihailovic R, O’Connor R, Khanin PL, ND, Decarolis Socci M, Hafner A, Jacobsen E, Brill S, Ugras Ther radix. platycodi standardized of activity antiangiogenic the of study J, Yu F. Twiner Gimble Greenway Z, pilot Liu Yand EM, Pharmacokinetic Med Prev Accelerometer steps/day translation of activity. moderate-to-vigorous TS. Church and PT WD, Katzmarzyk Johnson C, Tudor-Locke Leonardi C, survey. use time american the day: working the on behaviors sedentary and activity physical in PT. spent Time Katzmarzyk and WD Johnson C, Tudor-Locke Leonardi C, USin adults. accelerometer-determined steps/day and other accelerometer outputs PT. between Relationship Katzmarzyk and WD Tudor-Locke Johnson C, Health of wear time. impact accelerometer outputs: of time-stamped profile PT. population U.S. Katzmarzyk and WD Tudor-Locke Johnson C, Study. CANPLAY Surveillance the overweight/obesity: and time, watching and youth’s pedometer-determined steps/day, TV parent-reported children’s Canadian JM. Griffiths Cand Cameron Tudor-Locke CL, Craig C, 8:79. 2011; SCIENTIFIC REPORT 2011; 28(10):857-65. 2011; 8(5):693-8. Int J Behav Nutr Phys Nutr Act JBehav Int 2011; 53(1-2):31-3. Int J Behav Nutr Phys Nutr Act JBehav Int J Phys Act Health J Phys Act Int J Behav Nutr Phys Nutr Act JBehav Int 2011; 19(10):1987-98. 2011; J Occup Environ Med Environ J Occup 2011; 8(3):410-9. 2011;

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2011; 8:105. Circ Heart Fail Circ Heart 2 Heart Cancer Res Cancer 010–2011 2011; 8:78. 2011; 2011; 97(1):44-8. 2011; Proc Natl Acad Sci USA Acad Natl Proc Int J Behav Nutr Phys Nutr Act JBehav Int 2011; 71(17):5659-69. 2011; 53(12):1382-7. 2011; 2011; 4(5):607-12. 2011; J Phys Act J Phys Act Nat Med 2011; 2011; Adv

through IL15Ralpha receptor. effects antidepressive exerts and system serotonin Interleukin-15 affects Y, He Stone KP, AJ, RS, Khan Kastin H, Hsuchou PanWu W. and MS X, Cash weight. is losing who and is gaining who children: in changes weight body of study Longitudinal DW. Harsha TM and WD, Stewart Johnson H, Han DA, Williamson disease. Krabbe of model a murine of progression on injection cell stromal mesenchymal intrastriatal of Effect PJ. Pistell BA and Bunnell JM, Gimble X, Zhang BA, Trygg Scruggs CB, JM, J, Fisher-Perkins J, Klopf-Eiermann Dowden B, Zhang H, Londot SE, Wicks 46(5):1562-74. in QALYs. calculating preferences population versus patient of validation Empirical A. Gandjour and EJ Weyler mice. neuropathy in streptozotocin-diabetic onperipheral L., dracunculus PMI-5011, of Artemisia Evaluation of IG. Obrosova extract ethanolic an Watcho P, Tane R, Stavniichuk P, Y, Pand Maksimchyk H, Pacher Shevalye Biochem mice. diet-fed high-fat in muscle content skeletal in carnitine acyl enhance insulin melon signaling bitter and modulate from Bioactives YuWang XH, D, ZQ, WT. Zhang Floyd Cefalu Y, ZE and Ribnicky A, Poulev mice. KK-A(y) in muscle skeletal in expression gene modulates and signaling receptor insulin enhances L. dracunculus Artemisia of WT. extract An Yu I, Cefalu Raskin Yand A, D, Zuberi XH, Zhang Wang ZQ, Ribnicky 23(3):333-8. women. and men between Comparison tissues: and organs major of rates metabolic specific of Evaluation MJ. Muller SB and W, Later M, Heymsfield J, Heller Zhang A, Bosy-Westphal Z, Wang Ying Z, 23(3):333-8. women. and men between comparison tissues: and organs major of rates metabolic specific of Evaluation MJ. Muller SB and W, Later M, Heymsfield J, Heller Zhang A, Bosy-Westphal Z, Wang Ying Z, marrow. bone macaque rhesus from cells derived stem mesenchymal in changes Age-related BA. Bunnell and MA Freitas X, Guan JM, Yu Gimble Wu X, JM, cells. stem adipose-derived murine of Isolation Floyd ZE. and JM YD, Gimble Halvorsen G, Yu Kilroy Wu X, G, Mol Biol stem cells. differentiationof adipose-derived Adipogenic JM. Gimble and BM T, YD, Buehrer Yu Halvorsen Floyd Hebert Wu ZE, G, X, pancreatic damage. obesity-induced against chronic Eliminationprotects inflammasome of the NLRP3-ASC VD. D, Dixit Aand Burk B, Ravussin Vandanmagsar Youm A, Adijiang YH, 38(12):872-8. tissue by chronic inflammation. adipose in differentiation cell stem of Regulation Ye JM. Gimble Jand Diab Rep Ye in chronic role inflammation. J. tissue its Adipose vascularization: Sci Sci Med ABiol Gerontol individuals. uninfected and HIV-infected in is similar decline muscle skeletal C. Age-related Grunfeld and P Bacchetti SB, Heymsfield RA, DP, Kronmal CE, Kotler Lewis R, PC, Tien AS, Scherzer KE, Dobs Yarasheski inhibition of hepatic gluconeogenesis. gluconeogenesis. of hepatic inhibition by rats diabetic in metabolism glucose improves Weng J. Berberine Yan X, Xia Y, J, Shen Tang Y, J, Zhang Yin K, Yang D, Ye H, Liang Jand 2011; 27(3):299-307. 2011; Med JMol Int Biochem J Nutr PENNINGTON RESEARCH CENTER BIOMEDICAL 2011; 702:193-200. 2011; 22(11):1064-73. 2011; 2011; 11(3):203-10. 2011; Aging Cell 2011; 10(1):66-79. 2011; 2011; 22(1):71-8. 2011; 2011; 66(3):332-40. Obesity (SilverObesity Spring) Psychoneuroendocrinology 2011; Physiol 2011; Pharmacol Exp Clin Behav Brain Res Methods Mol Biol Endocrinology PLoS One PLoS Health Serv Res Serv Health 2011; 19(3):667-70. 2011; 6(2):e16556. 2011; 225(2):415-25. 2011; 152(11):4039-45. 2011; 2011; 2011; Biol JHum Am 2011; 702:29-36. 2011; 2011; Biol JHum Am 2011; 36(2):266-78. 2011; 2011; Methods Methods J Nutr J Nutr J Curr Curr PUBLICATIONS 119 - - Am J Clin Nutr 010–2011 2

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2011; 19(8):1568-73. PLoS One 2011; 93(6):1212-9. Am J Clin Nutr 2011; Obesity (Silver Spring) Obesity (Silver SCIENTIFIC REPORT SCIENTIFIC Zhang Z, Hu G, Caballero B, Appel L and Chen L. Habitual coffeecon sumption and risk of hypertension: a systematic review and meta-analy sis ofsis prospectiveobservational studies. Zhou Keenan J, MJ, Losso JN, Raggio AM, Shen L, McCutcheon KL, Tulley Blackman MRRT, and Martin RJ. Dietary whey protein decreases food intake and body fat in rats. Zhu N, Jacobs DR, Sidney S, Sternfeld Jr., B, Carnethon M, Lewis CE, CM,Shay Sood A and Bouchard mass modifies C. Fat the association of with symptom-limitedfat-free mass Coronary the in duration treadmill Artery Risk Development Adults (CARDIA) Study. in Young 2011; 94(2):385-91. Altamirano-RoblesZou S, Liedo P, L, Cruz-Enriquez Morice J, A, Ingram DK, Kaub K, Papadopoulos N and Carey JR. Recording lifetime behavior and movement in an invertebrate model. - - Arch Intern 2011; 31(5):1873-84. 2011; 2011; 301(4):E599-607. 2011; J Neurosci 2011; 152(5):1829-38. 2011; 28(6):652-7. 2011; Endocrinology Am Endocrinol J Physiol Metab

2011; 171(20):1811-8.

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Zhang F, Dong L, Zhang CP, Li B, Wen J, Gao W, Sun S, Lv F, Tian H, F, Sun S, Lv Gao J, Li B,W, Wen Dong L, Zhang CP, Zhang F, X and Hu G. Qi Z, J, Yang Increasing L, Zhang CL, Yu Tuomilehto prevalence of gestational diabetes mellitus in Chinese women from 2008. to 1999 Med Diabet Zhang HenaganJ, Inhibition J. TM, Gao of glyceroneogenesis and Z Ye by histone deacetylase 3 contributes lipodystrophy to in mice with adipose inflammation. tissue Jones M, Faouzi Kerman Louis JC, GW, IA, LaqueZhang Y, A, Nguyen P, RhodesMunzberg and C H. Leptin-receptor-expressing the neurons in dorsomedial median and sympa preoptic hypothalamus regulate area Zhang L, Ebenezer PJ, Dasuri K,Zhang L, Ebenezer Fernandez-Kim PJ, Mariappan Francis J, SO, Bruce-Keller J, N, Gao Z, Ye AJ and Keller JN. Aging associated is with stress implications for adipose adipose in hypoxia oxidative and tissue: function. thetic brown adipose circuits. tissue Zhang Y, Tuomilehto J, Jousilahti P, Wang Y, Antikainen R and Hu G. Y, Life Wang Jousilahti J, P, Tuomilehto Zhang Y, style factors on the risks of ischemic and hemorrhagic stroke. Med Journal ArticlesJournal 2011 120

PUBLICATIONS andChaptersBooks 2010 and Katzmarzyk PT, editors. PT, editors. Katzmarzyk and C In: Bouchard patient. overweight the of Evaluation GA. Bray 2010, p. 244 Wiley-Blackwell, Disease Cardiovascular editors. Association., Heart American and RH In: Eckel reduction. risk CVD and management Obesity GA. Bray ,PA: 2010,Philadelphia Saunders/Elsevier, 99-119. Liver Disease :Pathophysiology, Management Diagnosis, Fordtran’s and Sleisenger editors. Gastrointestinal and LJ, Brandt and LS Friedman M, Feldman MH, In: Sleisenger Obesity. GA. Bray 2010, 1119 xx, Wiley-Blackwell, Chichester, West Sussex: 4th ed., p. editors. C, Cockram Aand Flyvbjerg B, stein Gold RIG, fat? we get In: do Holt how weight: of Control GA. Bray 2010, 327-38.Wiley-Blackwell, and Children editors. WH, Dietz and ID Caterson PG, In: Kopelman weight. body to reduce clinically used Drugs G. Bray 2010, p. 409 Kinetics, Human IL: xxi, Champaign, ed. PT. Obesity and Physical Activity Katzmarzyk Cand Bouchard Science editor. C, Bouchard In: status. current obesity: of genomics and Genetics C. Bouchard Obesity ence: and Genes Translational and Biology Progress Molecular in C. Sci Bouchard Struble MB, editors. T, In: Wilson weight. ahealthy Templeachieving and GA Bray NJ, and understanding Obesity: CM. Bray Champagne and GA 2010, Kinetics, Human IL: 26-29.Champaign, Struble MB, editors. T, In: Wilson assessment. Temple for and GA Bray NJ, methods of overview status: Nutritional GA. Bray and CM Champagne 2010,Humana, 253-74. C and Katzmarzyk PT, editors. PT, editors. Katzmarzyk C and In: intake. Bouchard dietary and level Physical activity C. Earnest sity Obe and Physical PT, Activity editors. Katzmarzyk Cand Bouchard In: obesity. in disease heart and Church TJ. Physical activity 2010,Humana, 227-39. 2010, 240-44. 2010, Obesity and cal Activity PT, editors. Katzmarzyk Cand In: Bouchard mass. lean of maintenance and loss, weight activity, SB. Physical Heymsfield Physicians for Guide Nutrition T, In: Wilson it. of editors. low MB, Temple Struble and GA Bray NJ, and high the pressure: blood and Diet GA. Bray DW and Harsha Obesity and PT, editors. Katzmarzyk Cand In: Bouchard loss. weight pharmacological in activity physical of Role FL. Greenway Elsevier,MA: 2010. editors. R, Dana and JC Besharse DA, In: Dartt pupil. optics: Visual DH. PD McDougal and Gamlin 2010, Kinetics, Human IL: 210-13. Champaign, ed., . 2nd ed., Champaign, IL: Human Kinetics, 2010, Kinetics, Human IL: 281-83. Champaign, . 2nd ed., SCIENTIFIC REPORT : Academic Press, 2010, Press, : Academic 1-8. . 2nd ed., Champaign, IL: Human Kinetics, 2010, Kinetics, Human IL: 226-28. Champaign, . 2nd ed., . 3rd ed., Chichester, West Sussex ; Hoboken, N. J.: N. Chichester, ;Hoboken, West Sussex . 3rd ed., Progress in Molecular Biology and Translational Nutrition Guide for Physicians for Guide Nutrition Nutrition Guide for Physicians for Guide Nutrition . Dallas, TX: American Heart Association ; Association Heart American TX: . Dallas, . 2nd ed., Champaign, IL: Human Kinetics, Kinetics, Human IL: Champaign, . 2nd ed., , 2010.

Physical Activity and Obesity and Physical Activity . Totowa, N.J.: Humana, 2010, Humana, . Totowa, N.J.: 311-17. |

Physical Activity and Obesity and Physical Activity 2 010–2011 Encyclopedia of theEye of Encyclopedia Clinical Obesity in Adults Clinical Obesity Textbook of Diabetes of Textbook Metabolic Risk for Metabolic Physical Activity Physical Activity . Totowa, N.J.: Totowa,. N.J.: . Totowa, N.J.: Totowa,. N.J.: . 9th ed., ed., . 9th . Boston, Boston, . . 2nd ed., . 2nd ed., Physi . 2nd 2nd . . 2nd 2nd . - - . - - and Katzmarzyk PT, editors. PT, editors. Katzmarzyk and C In: Bouchard level. activity physical and T.Rankinen Genetics 2010, 85-88. Obesity and Activity PT, editors. Katzmarzyk Cand behavior. In: Bouchard tary seden and activity physical in differences ethnic U.S. RL. Newton and Cabo R, editors. editors. R, Cabo and AV, In: Everitt DG Couteur SIS, CALERIE. Rattan from mans: Results hu in outcomes behavioral and psychological physiological, on restriction caloric of effect The E. Ravussin Land Redman 2010, Kinetics, Human IL: 73-75.Champaign, C and Katzmarzyk PT, editors. PT, editors. Katzmarzyk C and In: Bouchard oxidation. substrate and Physical activity SR. Smith York: New Springer, 2010.ed., and Katzmarzyk PT, editors. PT, editors. Katzmarzyk and C In: Bouchard obesity. of cost PT. economic The Katzmarzyk editor. editor. C, In: McQueen embryopathy. daibetic in expression gene altered of Analysis JM. Salbaum Cand Kruger Pavlinkova G, C, Kappen 46-59. tor. In: Toth disease. PP, heart coronary and diabetes of treatment edi and prevention the in Tuomilehto Gand exercise Hu and J. Diet 2010, Pub Inc, Science 83-100. Physicians T,Wilson MB. Temple Struble and GA Bray NJ, 196-98. Obesity and ity Physical PT,- Activ editors. Katzmarzyk Cand In: Bouchard tion. transporta of mode and level Tudor-Locke Physical activity CE. 2010. Toth PP. Year The Disorders Lipid in Women in Diabetes Swahn EI. 2010, Kinetics, Human IL: 136-41. Champaign, ed., Obesity PT, editors. Katzmarzyk Cand In: Bouchard peptides. hypothalamic and level CD. Physical activity Morrison 2010, 79-96. Carbohydrates Health Nondigestible Digestive and editors. TM, Paeschke and WR In: Aimutis fibers. fermentable of with consumption associated effects health and hormones gut er Low RJ. Martin Mand Janes R, J, Senevirathene Zhou MJ, Keenan 2010, Kinetics, man 364-67. editors. PT, Katzmarzyk Cand In: Bouchard research. obesity and activity in physical Future C. directions Bouchard and PT Katzmarzyk 2010, Kinetics, Human IL: 53-56. Champaign, diovascular disease. In: editor. Swahn EI, disease. diovascular car riskof the on diabetes of impact gender-specific The G. Hu 393-404. Advances in Medicine and Biology LV, In: Berhardt risk. women’s and stroke Diabetes editor. G. Hu The YearThe Disorders Lipid in PENNINGTON RESEARCH CENTER BIOMEDICAL Comprehensive Toxicology . 2nd ed., Champaign, IL: Human Kinetics, 2010, Kinetics, Human IL: 155-58. Champaign, . 2nd ed., Physical Activity and Obesity and Physical Activity . Totowa, N.J.: Humana, 2010, Humana, 437 p. xxiii, N.J.: Totowa, . . 2nd ed., Champaign, IL: Human Kinetics, 2010, Kinetics, Human IL: Champaign, . 2nd ed., . 2nd ed., Champaign, IL: Human Kinetics, Kinetics, Human IL: Champaign, . 2nd ed., Calorie Restriction, Aging and Longevity and Aging Restriction, Calorie Physical Activity and Obesity and Physical Activity Physical Activity and Obesity and Physical Activity : Nova Science Pub Inc, 2010. Pub Inc, Science : Nova : Clinical Publishing Services, 2010, Services, Publishing : Clinical Physical Activity and Obesity and Physical Activity . Amsterdam: Elsevier,. Amsterdam: 2010. : Nova Science Pub Inc, 2010, Pub Inc, Science : Nova : Clinical Publishing Services, Services, Publishing : Clinical . 2nd ed., Champaign, IL: Hu IL: Champaign, . 2nd ed., Diabetes in Women in Diabetes Physical Activity and Physical Activity Nutrition Guide for Guide Nutrition : Wiley-Blackwell, : Wiley-Blackwell, . 2nd ed., . 2nd ed., . 2nd ed., . 2nd ed., . 2nd 2nd . Physical Physical . 1st 1st . : Nova Nova : ------PUBLICATIONS 121 - - - - - 010–2011 2

| Inc, 2011, 41-58. Inc, 2011,

. Barcelona: Elsevier . Barcelona: Elsevier Espana, Obesity Adipose-Derived : Cells Stem Childhood Obesity: Risk Factors, : Nova Science Pub Adipose-Derived : Methods and Cells Stem SCIENTIFIC REPORT SCIENTIFIC . Boca Raton, Enfield, N.H.: CRCPress; Science . Chichester, West Sussex West England. Chichester, ; Hoboken, NJ: Wiley- . New York, NY: Humana Press, 2011, 17-27. Humana Press, 2011, NY: . New York, . Chichester, West Sussex, UK: Wiley-Blackwell, 2011, ix, 159 ix, Sussex, West 159 . Chichester, UK: Wiley-Blackwell, 2011, Adipokines : Nova Science Inc, Pub 2011. Redman L and Ravussin E. Caloricrestriction and aging. Serrano- In: Rios M, M.and A. editors. OJ G-FJ, Obesity 2011, 349 - 68. Richards RJ, Melendez-Ramirez and CefaluInsulin sensitiz WT. LY ers vs. secretagogues first as line therapy for diabetes: rationale for A andclinical Rizza choice. Vella In: R, editors. Dilemmas inClinical Diabetes pages. Sarzynski M, Rankinen T and Bouchard and family C. studies Twin of training responses. Bouchard In: C, HoffmanEP and IOC Medi cal Commission., editors. Genetic and Molecular Aspects of Sport Performance 110-20. Blackwell, 2011, Segel CM. Childhood Obesity: Factors, Risk Health Effects,Preven and tion Interleukins adipokines. as KP. Stone Preedy In: VR and Hunter RJ, editors. ix, 448 p. Publishers, 2011, Obesity, J. inflammation andYe the metabolic syndrome. In: Ser rano-Rios M, M. and editors. A. OJ G-FJ, Espana, 2011, 169-88. Espana, 2011, X, G, ZE, Wu Halvorsen Floyd Yu YD and Gimble JM. Isolation of human adipose-derived stem from cells lipoaspirates. Gimble In: JM and Bunnell BA, editors. Protocols X, G, ZE, Wu Hebert Floyd HalvorsenYu Buehrer YD, T, BM and Gimble JM. Adipogenic differentiation of adipose-derived cells.stem GimbleIn: JM and Bunnell BA, editors. . New York, NY: Humana Press, 2011, 193-200. Methods and Humana Protocols Press, 2011, NY: . New York, X. Z, The Hu G and Yang prevalenceYu of overweight and obesity and related metabolic abnormalities among children and adoles cents in China. Segel In: CM, editor. Prevention and Effects, Health - - - . Boca . . New New . . Chich Genetic and . Heidelberg: Springer, Springer, Heidelberg: . . New York: Humana. New York: . Chichester, West Sussex, West . Chichester, UK: . 4th ed.. 4th Newtown, Handbooks PA: in Health . Barcelona: Elsevier Espana, 2011, 247-66. . Barcelona: Elsevier Espana, 2011, Obesity . Barcelona: Elsevier Espana, 2011, 47-64. . Barcelona: Elsevier Espana, 2011, . Boca Raton: CRC Press, 2011, xxxiv, 370 p., xxxiv, of plates. 4 p. 370 . Boca Raton: CRC Press, 2011, Contemporary Diagnosis and Management of Obesity of and Management and Diagnosis Contemporary A Guide Obesity to and Metabolic the : Originsand Syndrome . New York, NY: Humana Press, 2011, xv, 473 p. 473 xv, Humana Press, 2011, NY: . New York, Genetic and Molecular Aspects of Sport Performance Obesity Aesthetic Medicine : Art and Techniques

. Enfield, New Hampshire: Science Publishers, 2011, xii, 434p. xii, . Enfield, New Hampshire: SciencePublishers, 2011,

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON ester, West Sussex West Englandester, ; Hoboken, NJ: Wiley-Blackwell, 2011, 177-84. York: Springer, 2011, 741-55. 2011, Springer, York: Hsuchou H, He and W, Y Pan Kastin AJ. Astroglial leptin receptors and obesity. Preedy In: VR and Hunter RJ, editors. Adipokines Raton, Enfield, N.H.: CRC Press; Science Publishers, 2011, ix, Raton,448 Enfield,p. N.H.: CRCPress; Science Publishers, 2011, SarzynskiRankinen T, M and Bouchard C. Genes and response to training. Bouchard In: C, HoffmanEP and IOC MedicalCommission., editors. 2011, 255- 63. Kappen C. Vertebrate Hox genes and specializations in mammals. In: Evolu Animal in DeSalle R and Schierwater B, editors. Transitions Key tion Martin Chellino CK, A and Correa McClernon Food J. cravings: FJ, a central construct in food weight intake behavior, loss, and the neurobiology of appetitive Preedy In: behavior. VR, Watson RR and Martin CR, Nutrition and Food editors. Handbook Behavior, of Bouchard C, Hoffman E and IOC MedicalCommission. Greenway and Gutierrez FL B. Mesotherapy solutions for inducing lipolysis and treating Prendergast cellulite. In: and Shiffman PM MA, editors. ing the to obesity epidemic? Serrano-Rios In: M, M. and A. OJ G-FJ, editors. AspectsMolecular Sports of Performance p. Wiley-Blackwell Pub., 2011, Bray GA. Syndrome Metabolic the Care Co., 2011. Bray GA. Treatment Bray GA. guidelines Current on the identification,evaluation and treatment of overweight and obesity. Serrano-Rios In: M, M. and OJ A. editors. G-FJ, Champagne CM. Nutrition for the diabetic child. Ferry In: RJ, editor. Management Obesity of Pediatric and Diabetes 265-74. Press, 2011, Gimble JM and Bunnell BA. Adipose-Derived : Methods and Cells Stem Protocols Bouchard C. Genetics of obesity: are genetic differencescontribut Books and Chapters 2011 Books Chapters and OUR SUPPORTERS

PENNINGTON MEDICAL FOUNDATION

Board of Trustees: Ex-Officio

Paula Pennington de la Bretonne, Chair Steven Heymsfield, M.D. Executive Director, Pennington Jake L. Netterville, Vice Chairman Biomedical Research Center

G. Lee Griffin Secretary Charles Lamar Richard Lipsey William Hodgkins John V. Lombardi, Ph.D. Claude B. Pennington III President and Daryl B. Pennington, Sr. Chief Executive Officer Raymond G. “Skipper” Post William L. Silvia, Jr. William B. Richardson, Ph.D. Larry H. Hollier, M.D. Christel C. Slaughter, Ph.D.

122 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER POPULATIONOUR SUPPORTERS SCIENCE 123 010–2011 2

SCIENTIFIC REPORT SCIENTIFIC

Paula Pennington de la Bretonne la de Pennington Paula Chair Dear Friends, faithfully fulfilled the Medical have Foundation of the Pennington The Trustees grandparents of the funds being provided they by stewards astute of my intentions a world into Biomedical Center Research of the Pennington for development the leader the fieldand in diabetes obesity of research. Through carefulmanagement, program in support million for Pennington $165 provided has over the Foundation provide will Foundation the year, Biomedical its fiscal current In the inception. since its mission. accomplishing in the Center assist to million $3 over the funds provided donation, the Foundation the original by Over since the years the construction in resulted one-half and equipping of over have feet square million and support providedof research has Pennington addition, In the Foundation space. Biomedical with vital funding scientists, for research endowed start-up packages unrestricted faculty, for research funds development for its and Executive Director, support other numerous opportunities for state unavailable. funds which were Biomedical in focused has Pennington on assisting the Foundation Recently, The program. Foundation commercialization and transfer technology a developing providedhas funds for experts leading the field in intellectual of property workand with Center the to visit Center of the staffdiscoveries programsto to bringdevelop designedthe scientific and Center Foundation extraordinarily is This public the marketplace. importantto from two perspectives. First, bringing by the public, peoples’ to laboratories from Chronic and clinical improved. research the are lives discoveries unknown. previously were which Second, or the commercialization managed ways in prevented are diseases are importantexceedingly that streams revenue these significant in of technologies provides potentially stressful the state funded economic times. Through the leadership and the Legislature, of Governor Jindal into space of existing research and the renovation clinical Center, Building, Imaging Research a new Clinical As opportunities into these ideas facilities becomea childhood available, obesity put innovative to center. accelerate. will citizens action for the benefitLouisiana’s of and I served Copping together Trustees as Dr. Copping. Allen marks year of Dr. the passing this On a sad note, Biomedical about the Pennington passionate was Copping Dr. its 1980. in inception since of the Foundation President as and more importantly, both a Trustee as and, vision grandfather’s He shared my Center. Research during he instrumental and protecting was University guiding in the Center System, State of the Louisiana to be one BiomedicalCenter Pennington Research theconsidered Coppingalways precarious fiscaltimes. Dr. wit, direction wisdom, gentle his miss will advice. and all We grandestof his accomplishments. Sincerely, A MESSAGE FROM OUR FROM A MESSAGE FOUNDATION CHAIR FOUNDATION PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON PENNINGTON BIOMEDICAL RESEARCH FOUNDATION BOARD OF DIRECTORS OUR SUPPORTERS

Officers Directors Ex-Officio Directors

Tim A. Barfield, Jr. Clay M. Allen Steven B. Heymsfield, M.D. Chairman J. Herbert Boydstun Executive Director, Pennington J.S. “Si” Brown, III Biomedical Research Center John B. Noland J. Terrell Brown John V. Lombardi, Ph.D. Immediate Past-Chair Joseph “Jay” H. Campbell, Jr. President, LSU System Maxine Cormier Melvin “Kip” Holden J. Gerard “Jerry” Jolly Robert W. Daigle Mayor-President, Vice-Chair Paula P. de la Bretonne East Baton Rouge Parish C. Brent McCoy Paul M. Haygood Adam Knapp Treasurer C. Kris Kirkpatrick President/CEO, Baton Rouge Area Chamber Luther “Luke” C. Kissam, IV Annette D. Barton Charles A. Landry Janet L. Olson Secretary Carl S. Luikart, M.D Chair, Annual Fund for Excellence 2010-2011 Kevin R. Lyle Myron E. Moorehead, M.D. David A. Winkler Chair, Annual Fund for Elizabeth “Betsy” S. Nalty Excellence 2011-2012 George D. Nelson, Jr. Nanette Noland Jennifer G. Winstead President/CEO, Pennington Kevin P. Reilly, Jr. Biomedical Research Foundation Kevin P. Reilly, Sr. * Includes members from J. Brad Jewell Elizabeth Querbes Sammons January 1, 2010 until Chief Financial Officer, December 31, 2011 John Spain Pennington Biomedical Research Foundation

124 SCIENTIFIC REPORT | 2010–2011 PENNINGTON BIOMEDICAL RESEARCH CENTER POPULATIONOUR SUPPORTERS SCIENCE 125 010–2011 2

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Tim Barfield Chairman Board of the Dear Friends, pleased I am Biomedical Foundation, Research As of the Pennington chairman generosity the again once helping that of our donors to share contributed to has its lead the world in to work continue Biomedical Center Research the Pennington eliminating chronic diseases. Biomedical retain important enable Pennington to help donations scientific Private leaders gifts These research. and advance philanthropic vital are help to and support projects, pilot the health in chairs endow and professorships, and invest and well-being locally of citizens, and worldwide. Our donors helped also make to provided and and 2011, targeted recruitments faculty research of key 2010 in proceedbridge to research their funding existing while allowed to scientists that funding. outside for competing is Biomedical Center Research The taking the Pennington research at place more are grateful important the We to individuals, companies, and than ever. Biomedicaldiscovering fulfill of its helping Pennington are mission that foundations human improves that research the triggers innovative through diseases of chronic the lifespan. across health Sincerely, A MESSAGE FROM THE FROM A MESSAGE CHAIRMAN OF THE BOARD OF CHAIRMAN PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON 126

OUR SUPPORTERS Endowments Eminent Scholar: Phillip Brantley,Eminent Scholar: Ph.D. CoypuDonor: Foundation Trust StaufferMcIlhennyJohn Endowed Nutrition in Professorship M.D. Cefalu, William Eminent Scholar: Manship, L. Douglas Sr.Donor: Manship,Douglas L. Sr. Endowed Professorship in Diabetes ENDOWED PROFESSORSHIPS Ph.D. Eminent Peter Scholar: Katzmarzyk, LouisianaDonor: Public Facilities Authority LPFA Endowed Chair in Nutrition Ravussin, Eric Ph.D. Eminent Scholar: Educational Schlieder Foundation Edward G. Donor: Endowed Chair Gordon inandMetabolism Diabetes L. Douglas Eminent StevenM.D. Scholar: Heymsfield, PenningtonDonor: Foundation Medical Bray, A. George Jr. Endowed Chair Super in Nutrition Keller,Eminent Jeffrey Scholar: Ph.D. National Hibernia Bank One, formerly Capital Educational Schlieder Foundation Edward G. andDonors: Endowed Chair Schlieder G. NationalHibernia Bank/Edward Ph.D. M.D., Kastin, Eminent Abba Scholar: UnitedDonor: Companies Financial Corporation United Endowed Companies/Harris Chair J. Chustz Eminent Scholar: Jacqueline Stephens, Ph.D. Pennington “Doc” C.B. Donor: Claude B. Pennington, Jr. Endowed Chair ENDOWED CHAIRS (AS OFDECEMBER31,2011) (AS OFDECEMBER31,2011) SCIENTIFIC REPORT

010–2011 Eminent Scholar: Richard Rogers, Ph.D.Eminent Richard Rogers, Scholar: CoypuDonor: Foundation Trust Neuroscience McIlhenny S. John Endowed in Professorship Nutritional Eminent Ph.D. Scholar: Hans-Rudolf Berthoud, Bray and and Mitzi George FriendsDonors: Bray Endowed H. Professorship George Eminent Scholar: Under Recruitment andP.Noland, Foster Bailey B. andJamesVirginia J. and John III, Bailey Laura Donors: Disease Prevention Foster Endowed Bailey Fairfax Chair in Heart Eminent Claude Ph.D. Bouchard, Scholar: VariousDonors: (Friends andFamily W. ofJohn Sr.) Barton, W.John Sr. Barton, andNutrition Endowed Chair in Genetics Eminent Timothy Church, Scholar: M.P.H., M.D., Ph.D. CoypuDonor: Foundation Trust McIlhennyJohns S. Endowed Chair in Health Wisdom Ph.D. Kappen, Claudia Eminent Scholar: Foundation Michigan Community Southeastern for IreneDonors: W. Pennington & C.B. Foundation and the and theRisk ofObesity Pennington M. Peggy Cole Endowed Chair in Maternal Biology Eminent Scholar: Under Recruitment Foundation oftheLake Lady Our Donor: Diabetes EdanaMarie Corcoran and Endowed Chair in Pediatric Obesity PENNINGTON RESEARCH CENTER BIOMEDICAL POPULATIONOUR SUPPORTERS SCIENCE 127 * Deceased 010–2011

Educational Research Foundation Science of Advancement PARTNER and above $25,000 The Advocate Inc. Company, Agritech Ajinomoto American Medical Assoc. - Coca-Cola Rouge Baton Nan and Herb Boydstun Buquet & Leblanc, Inc. Louisiana of Digestive Foundation Health The Dow Company Chemical Gene and Duke Sylvia Anonymous The John Galt Fund Hernandez H. Mrs. John and Mr. Foundation BartonThe Family John W. Company Kellogg Kraft Foods North America, Inc. Latter Inc. & Blum, / C.J. Brown Realtors Long Law Firm, LLP CharitiesLouisiana Trust McMains Foundation Inc. J.Womack, Milton Anonymous Noesis Data, LLC Nanette Noland for the Foundation Orentreich Joanie and Allen Penniman The Charitable Pew Trusts Inc. Pfizer, LLC Placid RefiningCompany, Group Resources Hicks/ Provident Steve Jerry* Schwing and Chuck Group Inc.The Shaw Page andWilliam L. Silvia, Jr. The Josef Memorial Fund Sternberg Moo and Martin Svendson Phenex Pharmaceuticals AG Phenex Pharmaceuticals Phytomedics, Inc. *Norma Raiford Jean Inc. Roche Laboratories, RoyOMartin Foods Company Slim Fast and JerryJohn G. G. Turner Fischer University Inc. of Colorado Foundation, PartnersWestlake Hospital Woman’s SCIENTIFIC REPORT SCIENTIFIC

Company Fund Company of Louisiana Medical Center Fund Memorial Mr. and Mrs. Bill Dixon Mr. Lamar III and Mrs. W. Charles Mr. Foundation Patsy and Lawrence D. Adcock Patsy and Lawrence D. Inc. Pharmaceuticals, Amylin Laura Bailey III J. and James Nan and Tim Barfield Annette Barton D. Mitzi M.D. and George A. Bray, Brown Mary Terrell and J. Kay for ObesityCenters & Education Research CIGNA Foundation Coates and Mrs. Dudley W. Mr. Rouge of Baton MedicalColumbia Center Lev Dawson Bretonne de la P. and Paula Jacques Dr. ExxonMobil GeneralHealth System Guaranty Corporation Anne and Bill Hise Chemical S. - Uniroyal Simon Hovey PHILANTHROPIST and above $50,000 Benjamin KleinpeterD. Charles and Josephine Lamar Hancock Bank and Trust Company Company Hancock Bank and Trust William Hansel Dr. Corporation – BASF Knoll Pharmaceutical Lamar Advertising Company and Don Lyle Pat McCoy W. Ruth and *Charles *Margaret C. Moore Our Lady of the Lake Regional *Bert S. Turner and Sue Mr. and Mrs. Charles W. Lamar III and Mrs. W. Charles Mr. BallooningLouisiana Foundation R.Kevin Lyle Sr. *Douglas L. Manship, Mars, Incorporated Medical University of South Carolina The Support Milford Wampold Inc. Nutrition 21, Rouge of Baton Ochsner Medical Center Anonymous

PENNINGTON BIOMEDICAL CENTER RESEARCH PENNINGTON Premier Bank andPremier LNB ChustzHarris J. Foundation Southeastern Michigan Foundation

formerly Bankformerly One, City National Bank, formerly Hiberniaformerly National Bank Laura and James Bailey J. Laura III B. andVirginia John B. Noland Bailey Foster *P. in memoryin Brown L. Heidel of Albemarle Foundation Amedisys, Inc. Loretta M. M. and Edward Downey LillyEli and Company Anonymous Gordon & Mary Foundation Cain PREMIER BENEFACTOR PREMIER above and $100,000 PREEMINENT BENEFACTOR PREEMINENT and above $250,000 of Louisiana and Blue Shield Blue Cross Charles Lamar Family The Foundation Reilly Family Authority PublicLouisiana Facilities United Companies Financial Corporation/ The Coca-Cola Company Corporation Entergy JPMorgan Chase Bank John W. Barton, Sr. John W. OneCapital

Alta and John Franks Foundation Bailey and Noland Families PRESIDENT’S CIRCLE above and $500,000 Virginia B. and JohnVirginia B. Noland Our Lady of the Lake Foundation Community Foundation for for Foundation Community Trust Foundation Coypu G. Edward Schlieder Educational and C.B. Pennington Irene W.

PENNINGTON SOCIETY PENNINGTON above and $1,000,000 and *C.B. Pennington *Irene W. Foundation Area Rouge Baton *ImogeneN. Brown Our Donors – Honoring Lifetime Giving Our Lifetime Honoring – Donors 128

OUR SUPPORTERS Donors–HonoringLifetimeOur Giving Guaranty Broadcasting Company of Broadcasting Guaranty Griffin andLee Barrie Greer, S. Sr.*Patricia and*Robert GlaxoSmithKline Lynnette andTommy Frazer Fisher Charles Pam and Equitas Capital Advisors, LLC Ellison, andDavid M. Jr.Kathryn Edgen Murray Corporation Aglonie DiVincenti, Jr. S. andMichael Corporate Management ofBaton Cormier Maxine Children’s Hospital The Cassidy M. William and Laura Drs. ofBaton Bank Rouge Business First Bruce Foods Corporation LLP Wilson, & Sachse Breazeale, Marilyn Braymer Braymer, Douglas H. Ph. D. Jane T.Mrs. Boyce Dr. Claude andMonique Bouchard Jeanie andDavid Bondy Dr. Drake E. Bellanger CenterBaton Medical General Rouge Anonymous Anonymous Associated Food Stores J. Gallagher Risk ManagementArthur Inc. Amgen, theMove on America Corporation ALaw Allen &Gooch, LLP Adams Reese, and InternationalABIC Consultants Abbott Anne andHerschel $10,000 above and PATRON Wurtele Joanna and*MiltonMargaret C. J. Womack WHLC Architecture W.R. Grace&Company Anonymous Turner Group, LLC Industries ToupsKay M. andRoland Takeda North Pharmaceuticals SCIENTIFIC REPORT Baton Rouge, LLC Rouge, Inc. Chagnon Inc. Services, Inc. America,

010–2011

*Mr. D. Joe Smith andMrs. Steven D. Smith, M. R. and Amerique Servier Janice andFrank Sadler Edwin Lupberger and M.D. Ryan, H. Donna Mr. Kevin P. andMrs. Reilly, Jr. APAC Netterville, Postlethwaite & Post, Jr. G. andRaymond Bryan Architects Post Phillips Gary and Claudia Inc. Contractors, Performance Pamlab, LLC Inc. Novo Nordisk, the for Association American North Noland Nanette FoundationThe Mr. Nolan James M. Council Dairy National Miss Teen USA -Miss Universe LynchMerrill Grigsby Johnson “Boo” Merice The &Co.,Merck Inc. Nutritionals McNeil McLaurin Lee Phyllis and *John McIlhenny S. McCoy Brent Bird Perkins CancerMary Center Dr. Roy J. Martin andMrs. Martin Jonathan and Maggie Louisiana Machinery Foundation, Louisiana Diabetes Susan andRichard Lipsey Jr. andWalterAnne C. Legett, E. Anonymous LLP KPMG, Kadair, andRoyMargo G. M.D. WalkerJones Jolly andJerry Donna Johnson Dr. A. Sheldon andMrs. CuretJean H. FundResearch Medical for J.B. James Construction Bank Investar Hise Richard andDebra Hill, andJohn Cindy Jr. Ava and Cordell Haymon Joffa Braymer, Joffa M.D. Study ofObesity Organization Foundation Favre Fund Research Lipe &

PENNINGTON RESEARCH CENTER BIOMEDICAL

Margaret C. Womack Hart C. Margaret William Wright, H. Jr.. Womack *Barbara andChuckJennifer Winstead Trust Charitable Montan E. William Wilkinson Ann Market, Inc. Foods Whole WBRZ-TV 2 Channel USDA Plantation Club University ArmyU.S. Research Institute of *Charlotte Thompson M. and*J.*Ida Frank Terrell, Jr. Taylor Firm Law Porter Mr. Sullivan Leonard andMrs. Place St. James LLC Capital, Source Crestar FinancialCorporation Crestar Covidien Health CharitiesCommunity ofLA Communications Channel Clear SocialCity Magazine Patricia Cheramie L. Ph.D., Champagne, R.D. Catherine Cefalu William and Elizabeth Inc. PharmaceuticalCatalyst Partners, Union Credit Campus Federal Jr.Mr. Campbell, J.H. andMrs. Jr. Campbell, S. andJohn Rosemary Calhoun RuthS. *Mrs. BoyceMelanie andJohn Beskin Jeanelle Mechanical Brothers Bernhard CenterBaton Cardiology Rouge Barton Scott Mary Jr. Barton, andJohn Peggy VernonBarrett, & Montgomery, LLC Princeton andDadie Bardwell Foundation Arthritis Stress Analytic ofClinical Association American Saurage, Jr. N. Alma andH. Lee The Fund Anonymous $5,000 above and PACESETTER Environmental Medicine and MS and Contractors Endocrinologists * Deceased

OUR SUPPORTERS 129

* Deceased

010–2011 2

The Pennington The Pennington University System University System 234-acre campus.

the Louisiana State State Louisiana the and conducts basic, basic, and conducts research. More than than More research. Center is a campus of of a campus is Center Biomedicatl Research Research Biomedicatl 500 employees occupy clinical and population and population clinical

several buildings on the the on buildings several

CETFCREPORT SCIENTIFIC

201 2011 – 0

PENNINGTON BIOMEDICAL RESEARCH CENTER RESEARCH BIOMEDICAL PENNINGTON SCIENTIFIC REPORT | 2010–2011

6400 Perkins Road Baton Rouge, Louisiana 70808 Telephone: 225-763-2500 www.pbrc.edu