DOCSLIB.ORG

SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems Dipòsit Legal: T. 1717-2011 ADVERTIMENT. La consulta d’aquesta tesi queda condicionada a l’acceptació de les següents condicions d'ús: La difusió d’aquesta tesi per mitjà del servei TDX (www.tesisenxarxa.net) ha estat autoritzada pels titulars dels drets de propietat intel·lectual únicament per a usos privats emmarcats en activitats d’investigació i docència. No s’autoritza la seva reproducció amb finalitats de lucre ni la seva difusió i posada a disposició des d’un lloc aliè al servei TDX. No s’autoritza la presentació del seu contingut en una finestra o marc aliè a TDX (framing). Aquesta reserva de drets afecta tant al resum de presentació de la tesi com als seus continguts. En la utilització o cita de parts de la tesi és obligat indicar el nom de la persona autora. ADVERTENCIA. La consulta de esta tesis queda condicionada a la aceptación de las siguientes condiciones de uso: La difusión de esta tesis por medio del servicio TDR (www.tesisenred.net) ha sido autorizada por los titulares de los derechos de propiedad intelectual únicamente para usos privados enmarcados en actividades de investigación y docencia. No se autoriza su reproducción con finalidades de lucro ni su difusión y puesta a disposición desde un sitio ajeno al servicio TDR. No se autoriza la presentación de su contenido en una ventana o marco ajeno a TDR (framing). Esta reserva de derechos afecta tanto al resumen de presentación de la tesis como a sus contenidos. En la utilización o cita de partes de la tesis es obligado indicar el nombre de la persona autora. WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reproduction with lucrative aims is not authorized neither its spreading and availability from a site foreign to the TDX service. Introducing its content in a window or frame foreign to the TDX service is not authorized (framing). This rights affect to the presentation summary of the thesis as well as to its contents. In the using or citation of parts of the thesis it’s obliged to indicate the name of the author. UNIVERSITAT ROVIRA I VIRGILI CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems DL. T. 1717-2011 Haybrig Perdomo Ziems CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES TESIS DOCTORAL Dirigida por Josep Guarro, Josepa Gené y José F. Cano Departament de Ciències Mèdiques Bàsiques Reus 2011 UNIVERSITAT ROVIRA I VIRGILI CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems DL. T. 1717-2011 FACULTAT DE MEDICINA I CIÈNCIES DE LA SALUT DEPARTAMENT DE CIÈNCIES MÈDIQUES BÀSIQUES UNITAT DE MICROBIOLOGIA Carrer Sant Llorenç 21 43201 Reus Tel. 34-977759321 Fax. 34- 977759322 Josep Guarro Artigas, Catedrático de Microbiología de la Facultat de Medicina i Ciències de la Salut, Josepa Gené Díaz, Profesora Titular de la Facultat de Medicina i Ciències de la Salut, y José F. Cano Lira, Catedrático de Microbiología de la Facultat d´Enologia, de la Universitat Rovira i Virgili HACEN CONSTAR: Que la presente tesis doctoral titulada “Caracterización fenotípica y molecular de hongos filamentosos oportunistas: Scedosporium, Acremonium, Phialemonium, Lecythophora y Paecilomyces” presentada por Haybrig Mercedes Perdomo Ziems, ha sido realizada bajo su dirección y cumple los requisitos necesarios para optar por el título de Doctor. Y para que así conste y tenga los efectos oportunos, firman la presente. Reus, 10 de Julio de 2011 Josep Guarro Artigas Josepa Gené Díaz José F. Cano Lira Director de la tesis Codirector Codirector UNIVERSITAT ROVIRA I VIRGILI CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems DL. T. 1717-2011 A mi Madre A Sergio “Cada trecho recorrido enriquece al peregrino y lo acerca un poco más a hacer realidad sus sueños”. Paulo Coelho UNIVERSITAT ROVIRA I VIRGILI CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems DL. T. 1717-2011 AGRADECIMIENTOS Este aparatado está dedicado a todas aquellas personas que me han acompañado durante estos 4 años… Al Dr. Josep Guarro, el jefe celestial, muchas gracias por todos los momentos compartidos, los buenos y los malos, por ser siempre el ejemplo al trabajo constante, por ser mi guía, mi empujón y muchas veces, mí martirio… Son tantos los aprendizajes, que las palabras se quedan cortas. Gracias de corazón. A la Dra. Josepa Gené, gracias por todo el tiempo invertido en la corrección de esta tesis y de todos los trabajos generados durante este tiempo. Su meticulosidad y dedicación es un ejemplo a seguir. A la Dra. Dania García, si enumero todas las cosas que me has enseñado no terminaría nunca!, gracias por el gran apoyo incondicional, por enseñarme esos “truquitos morfológicos” que sólo la experiencia aporta, por enseñarme a ser la “Sherlock Holmes” de la micología, por ser la chispa constante de las grandes ideas, por todos los momentos compartidos: los de risas, los de llantos, los de injusticia, los de ánimo… pufff…. Gracias. Al Dr. Josep Cano, por su confianza y apoyo, por esos: como lo tenemos?, por esos momentos de enojos, de risa y de complicidad, gracias por el cariño. A los Drs. Alberto Stchigel, Francesc Xavier Pastor, María José Figueras y Emilio Mayayo, por su disponibilidad de aclarar siempre las dudas planteadas y corregir los errores. Quiero agradecer especialmente a Beto por esas charlas tan animadas, llenas de risas y de ocurrencias, que hacían que el trabajo detrás del microscopio fuese ameno y divertido y al Dr. Pastor por escuchar mis tonterías mientras “el vecino” se desocupaba. A la Dra. D. A. Sutton por permitirnos trabajar con esos tesoros llamados “aislados” y por sus grandes aportes. Al Dr. Richard Summerbell por sus acertados aportes científicos y especialmente gracias por esas palabras que me han servido de aliento y energía para culminar este ciclo. A Núria Pilas, Cati Nuñez y Carme Sanmartí, porque el mejor OA del mundo sale de sus manos, por esas ayudas maravillosas, por esos momentos de risas a la hora del café/té, gracias. A las Drs. Roxana Báez y Anabel Alperi por la enseñanza, el apoyo, la ayuda y la amistad. Al Dr. Hugo Madrid (madrilito) y Eduardo Álvarez (el niño), mis compañeros de despacho, gracias por todos los momentos que durante estos 4 años hemos compartido, por el silencio, las charlas filosóficas, científicas, las risas, la complicidad, el apoyo incondicional. Ustedes y el amarillo siempre permanecerán en mis mejores recuerdos, los adoro. A las amigas que la vida me ha regalado durante estos años de doctorado: Fabiola Fernández, mi loca adorada, mi amiga querida; Jessica Pérez, mi osita; Mónica Azor, monii; Carolina Silvera, mi caracolilla; Valentina Salas, locaaa…. gracias, son parte de mi vida y ocupan un lugar muy importante de mi corazón. A mi amiga/madre Claudia Hartung por ser mi fuente de buena energía y optimismo, por escucharme y estar siempre conmigo en los momentos difíciles. UNIVERSITAT ROVIRA I VIRGILI CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems DL. T. 1717-2011 A todas aquellas personas con las cuales he compartido el maravilloso mundo del laboratorio: Xavi, Luis, Marçal, Félix, Mar, Enrique, Yasmina, Margarita, Sarah, Keith, Arturo, Katihuska, Max, Catañeda (mi padre), Julio… A Montse Pallas, Immaculada Abello, Amparo, María, Teresa… gracias. A mi alma mater, por ser la luz que siempre ha vencido a la sombra. A los Profesores Vilma Llovera, Rosa Cristina Pérez, Armando Suarez, Juan Luis León y Elizabeth Ferrer, por sus consejos y por el cariño. A todo el personal administrativo, técnico y profesoral del Departamento de Microbiología de la Universidad de Carabobo, La Morita. A la familia con la que España me ha bendecido, Antonio, Carmen, Tony, Patri, gracias por el apoyo, el cariño, por esos momentos compartidos alrededor de la mesa, por darme ese calor y la estabilidad que sólo una familia puede dar, gracias! A mi familia, mis tíos, primos, ahijados-sobrinos, a la memoria de mi tía Cruz, a mi tía Mercedes, a mi padre, a mis hermanos: Julio Ángel, Amilkar y José Antonio, a mi sobrino Víctor Julio, y especialmente a ti mamá, los amo con todo mi corazón, son mi inspiración, mi fuerza, mi puerto seguro, sin palabras…! A Sergio, mi ilusión, mi compañero, mi amigo, mi cómplice, hemos transitado este largo camino tomados de la mano, sin tu amor hubiera sido imposible culminar esta meta. Me has aportado todo lo que he deseado y más, me has dado todo lo que he deseado y más, has llenado de luz y de risas cada días que pasamos juntos, sabes lo importante que eres para mí, tú eres el autor de mi felicidad. T.A. UNIVERSITAT ROVIRA I VIRGILI CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems DL. T. 1717-2011 ÍNDICE UNIVERSITAT ROVIRA I VIRGILI CARACTERIZACIÓN FENOTÍPICA Y MOLECULAR DE HONGOS FILAMENTOSOS OPORTUNISTAS: SCEDOSPORIUM, ACREMONIUM, PHIALEMONIUM, LECYTHOPHORA Y PAECILOMYCES Haybrig Mercedes Perdomo Ziems DL. T. 1717-2011 ÍNDICE DE ABREVIATURAS………………………………………………………….. i 1. INTRODUCCIÓN……………………………………………………………………... 1 1.1. Generalidades de los hongos filamentosos…………………………………. 2 1.2. La biología molecular aplicada a la taxonomía y tipado de los hongos filamentosos………………………………………………………………….
Load more

Recommended publications
  • Mycosphere Notes 225–274: Types and Other Specimens of Some Genera of Ascomycota
    Mycosphere 9(4): 647–754 (2018) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/9/4/3 Copyright © Guizhou Academy of Agricultural Sciences Mycosphere Notes 225–274: types and other specimens of some genera of Ascomycota Doilom M1,2,3, Hyde KD2,3,6, Phookamsak R1,2,3, Dai DQ4,, Tang LZ4,14, Hongsanan S5, Chomnunti P6, Boonmee S6, Dayarathne MC6, Li WJ6, Thambugala KM6, Perera RH 6, Daranagama DA6,13, Norphanphoun C6, Konta S6, Dong W6,7, Ertz D8,9, Phillips AJL10, McKenzie EHC11, Vinit K6,7, Ariyawansa HA12, Jones EBG7, Mortimer PE2, Xu JC2,3, Promputtha I1 1 Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand 2 Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming 650201, China 3 World Agro Forestry Centre, East and Central Asia, 132 Lanhei Road, Kunming 650201, Yunnan Province, People’s Republic of China 4 Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, Yunnan 655011, China 5 Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China 6 Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand 7 Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand 8 Department Research (BT), Botanic Garden Meise, Nieuwelaan 38, BE-1860 Meise, Belgium 9 Direction Générale de l'Enseignement non obligatoire et de la Recherche scientifique, Fédération Wallonie-Bruxelles, Rue A.
    [Show full text]
  • Monograph on Dematiaceous Fungi
    Monograph On Dematiaceous fungi A guide for description of dematiaceous fungi fungi of medical importance, diseases caused by them, diagnosis and treatment By Mohamed Refai and Heidy Abo El-Yazid Department of Microbiology, Faculty of Veterinary Medicine, Cairo University 2014 1 Preface The first time I saw cultures of dematiaceous fungi was in the laboratory of Prof. Seeliger in Bonn, 1962, when I attended a practical course on moulds for one week. Then I handled myself several cultures of black fungi, as contaminants in Mycology Laboratory of Prof. Rieth, 1963-1964, in Hamburg. When I visited Prof. DE Varies in Baarn, 1963. I was fascinated by the tremendous number of moulds in the Centraalbureau voor Schimmelcultures, Baarn, Netherlands. On the other hand, I was proud, that El-Sheikh Mahgoub, a Colleague from Sundan, wrote an internationally well-known book on mycetoma. I have never seen cases of dematiaceous fungal infections in Egypt, therefore, I was very happy, when I saw the collection of mycetoma cases reported in Egypt by the eminent Egyptian Mycologist, Prof. Dr Mohamed Taha, Zagazig University. To all these prominent mycologists I dedicate this monograph. Prof. Dr. Mohamed Refai, 1.5.2014 Heinz Seeliger Heinz Rieth Gerard de Vries, El-Sheikh Mahgoub Mohamed Taha 2 Contents 1. Introduction 4 2. 30. The genus Rhinocladiella 83 2. Description of dematiaceous 6 2. 31. The genus Scedosporium 86 fungi 2. 1. The genus Alternaria 6 2. 32. The genus Scytalidium 89 2.2. The genus Aurobasidium 11 2.33. The genus Stachybotrys 91 2.3. The genus Bipolaris 16 2.
    [Show full text]
  • Culture Inventory
    For queries, contact the SFA leader: John Dunbar - [email protected] Fungal collection Putative ID Count Ascomycota Incertae sedis 4 Ascomycota Incertae sedis 3 Pseudogymnoascus 1 Basidiomycota Incertae sedis 1 Basidiomycota Incertae sedis 1 Capnodiales 29 Cladosporium 27 Mycosphaerella 1 Penidiella 1 Chaetothyriales 2 Exophiala 2 Coniochaetales 75 Coniochaeta 56 Lecythophora 19 Diaporthales 1 Prosthecium sp 1 Dothideales 16 Aureobasidium 16 Dothideomycetes incertae sedis 3 Dothideomycetes incertae sedis 3 Entylomatales 1 Entyloma 1 Eurotiales 393 Arthrinium 2 Aspergillus 172 Eladia 2 Emericella 5 Eurotiales 2 Neosartorya 1 Paecilomyces 13 Penicillium 176 Talaromyces 16 Thermomyces 4 Exobasidiomycetes incertae sedis 7 Tilletiopsis 7 Filobasidiales 53 Cryptococcus 53 Fungi incertae sedis 13 Fungi incertae sedis 12 Veroneae 1 Glomerellales 1 Glomerella 1 Helotiales 34 Geomyces 32 Helotiales 1 Phialocephala 1 Hypocreales 338 Acremonium 20 Bionectria 15 Cosmospora 1 Cylindrocarpon 2 Fusarium 45 Gibberella 1 Hypocrea 12 Ilyonectria 13 Lecanicillium 5 Myrothecium 9 Nectria 1 Pochonia 29 Purpureocillium 3 Sporothrix 1 Stachybotrys 3 Stanjemonium 2 Tolypocladium 1 Tolypocladium 2 Trichocladium 2 Trichoderma 171 Incertae sedis 20 Oidiodendron 20 Mortierellales 97 Massarineae 2 Mortierella 92 Mortierellales 3 Mortiererallales 2 Mortierella 2 Mucorales 109 Absidia 4 Backusella 1 Gongronella 1 Mucor 25 RhiZopus 13 Umbelopsis 60 Zygorhynchus 5 Myrmecridium 2 Myrmecridium 2 Onygenales 4 Auxarthron 3 Myceliophthora 1 Pezizales 2 PeZiZales 1 TerfeZia 1
    [Show full text]
  • Phialemonium Obovatum Keratitis After Penetration Injury of the Cornea
    Korean J Ophthalmol 2012;26(6):465-468 http://dx.doi.org/10.3341/kjo.2012.26.6.465 pISSN: 1011-8942 eISSN: 2092-9382 Case Report Phialemonium obovatum Keratitis after Penetration Injury of the Cornea Kwon Ho Hong1, Nam Hee Ryoo2, Sung Dong Chang1 1Department of Ophthalmology, Keimyung University School of Medicine, Daegu, Korea 2Department of Laboratory Medicine, Keimyung University School of Medicine, Daegu, Korea Phialemonium keratitis is a very rare case and we encountered a case of keratitis caused by Phialemonium obo- vatum (P. obovatum) after penetrating injury to the cornea. This is the first case report in the existing literature. A 54-year-old male was referred to us after a penetration injury, and prompt primary closure was performed. Two weeks after surgery, an epithelial defect and stromal melting were observed near the laceration site. P. obo- vatum was identified, and then identified again on repeated cultures. Subsequently, Natacin was administered every two hours. Amniotic membrane transplantation was performed due to a persistent epithelial defect and impending corneal perforation. Three weeks after amniotic membrane transplantation, the epithelial defect had completely healed, but the cornea had turned opaque. Six months after amniotic membrane transplantation, visual acuity was light perception only, and corneal thinning and diffuse corneal opacification remained opaque. Six months after amniotic membrane transplantation, visual acuity was light perception only, and corneal thin- ning and diffuse corneal opacification remained. Key Words: Corneal ulcer, Fungi, Phialemonium obovatum Members of the Phialemonium genus are dematiaceous Case Report fungi, which are known as causative fungi for opportunis- tic infection in immunocompromised hosts.
    [Show full text]
  • University of Alberta Microfungus Collection and Herbarium (Uamh)
    UNIVERSITY OF ALBERTA MICROFUNGUS COLLECTION AND HERBARIUM (UAMH) A Unit of the Devonian Botanic Garden, Faculty of Agriculture, Life and Environmental Sciences Telephone 780‐987‐4811; Fax 780‐987‐4141; e‐mail: [email protected] http://www.uamh.devonian.ualberta.ca SUMMARY OF ACTIVITIES FOR 2011 Supporting fungal research for over 50 years Staff, Volunteers Professor Emeritus (Curator) ‐ L. Sigler Devonian Botanic Garden/UAMH, Fac. Agriculture, Life & Environmental Sciences Medical Microbiology & Immunology, Fac. of Medicine Adj. Prof. Biol. Sci. Consultant in Mycology, PLNA/UAH Microbiology & Public Health Assistant Curator (.5 FTE Non‐academic, .5 FTE trust, NSERC) – C. Gibas Technicians (trust): ‐ A. Anderson; V. Jajczay (casual) Volunteer‐ M. Packer Affiliates R. Currah, Professor Emeritus, Biological Sciences, Faculty of Science M. Berbee, Professor, University of British Columbia, Vancouver G. Hausner, Assistant Professor, University of Manitoba, Winnipeg Cultures Received, Distributed and Accessioned Cultures received for identification, deposit or in exchange (Table 1) ....................................... 233 Cultures distributed on request or in exchange (Table 2)............................................................ 262 Culture Collection and Herbarium Accessions Accessions processed to Dec 31. .................................................................................................. 284 Total accessions.......................................................................................................................
    [Show full text]
  • Taxonomic Re-Examination of Nine Rosellinia Types (Ascomycota, Xylariales) Stored in the Saccardo Mycological Collection
    microorganisms Article Taxonomic Re-Examination of Nine Rosellinia Types (Ascomycota, Xylariales) Stored in the Saccardo Mycological Collection Niccolò Forin 1,* , Alfredo Vizzini 2, Federico Fainelli 1, Enrico Ercole 3 and Barbara Baldan 1,4,* 1 Botanical Garden, University of Padova, Via Orto Botanico, 15, 35123 Padova, Italy; [email protected] 2 Institute for Sustainable Plant Protection (IPSP-SS Torino), C.N.R., Viale P.A. Mattioli, 25, 10125 Torino, Italy; [email protected] 3 Department of Life Sciences and Systems Biology, University of Torino, Viale P.A. Mattioli, 25, 10125 Torino, Italy; [email protected] 4 Department of Biology, University of Padova, Via Ugo Bassi, 58b, 35131 Padova, Italy * Correspondence: [email protected] (N.F.); [email protected] (B.B.) Abstract: In a recent monograph on the genus Rosellinia, type specimens worldwide were revised and re-classified using a morphological approach. Among them, some came from Pier Andrea Saccardo’s fungarium stored in the Herbarium of the Padova Botanical Garden. In this work, we taxonomically re-examine via a morphological and molecular approach nine different Rosellinia sensu Saccardo types. ITS1 and/or ITS2 sequences were successfully obtained applying Illumina MiSeq technology and phylogenetic analyses were carried out in order to elucidate their current taxonomic position. Only the Citation: Forin, N.; Vizzini, A.; ITS1 sequence was recovered for Rosellinia areolata, while for R. geophila, only the ITS2 sequence was Fainelli, F.; Ercole, E.; Baldan, B. recovered. We proposed here new combinations for Rosellinia chordicola, R. geophila and R. horridula, Taxonomic Re-Examination of Nine R. ambigua R.
    [Show full text]
  • Colonization of Vines by Petri Disease Fungi, Susceptibility of Rootstocks To
    PLANT PATHOLOGY / SCIENTIFIC ARTICLE DOI: 10.1590/1808-1657000882017 Colonization of vines by Petri disease fungi, susceptibility of rootstocks to Phaeomoniella chlamydospora and their disinfection Colonização de videiras pelos fungos da doença de Petri, suscetibilidade de porta-enxertos ao fungo Phaeomoniella chlamydospora e sua desinfecção Ana Beatriz Monteiro Ferreira1, Luís Garrigós Leite1, José Luiz Hernandes2, Ricardo Harakava3, Carlos Roberto Padovani4, César Junior Bueno1* ABSTRACT: Petri disease is complex, attacks young RESUMO: A doença de Petri é complexa, ataca plantas jovens vine plants and it is difficult to be controlled. The fungus de videira e é difícil de ser controlada. O fungo Phaeomoniella Phaeomoniella chlamydospora (Phc) has been identified as chlamydospora é o principal agente causal dessa doença. Os obje- the main causative agent of this disease. This study aimed to tivos deste estudo foram: avaliar o local prevalente dos fungos da evaluate the prevalent colonization of the Petri disease fungi doença de Petri, em diferentes partes de plantas de videira; ava- in different portions of vine plants; to assess the susceptibility liar a suscetibilidade de porta-enxertos de videira para o fungo of grapevine rootstocks to the fungus P. chlamydospora; to P. chlamydospora; avaliar o efeito da solarização e da biofumiga- assess the effect of solarization and biofumigation, followed by ção seguido de tratamento com água quente sobre a desinfecção hot-water treatment (HWT), on the disinfection of cuttings de estacas do porta-enxerto IAC 766 infectadas com o fungo of the rootstock IAC 766 infected with P. chlamydospora, and P. chlamydospora; avaliar o efeito da solarização e da biofumigação assess the effect of solarization and biofumigation, followed by seguido de tratamento com água quente sobre o enraizamento de HWT, on the rooting of cuttings of the rootstock IAC 766.
    [Show full text]
  • The Mycobiome of Symptomatic Wood of Prunus Trees in Germany
    The mycobiome of symptomatic wood of Prunus trees in Germany Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften (Dr. rer. nat.) Naturwissenschaftliche Fakultät I – Biowissenschaften – der Martin-Luther-Universität Halle-Wittenberg vorgelegt von Herrn Steffen Bien Geb. am 29.07.1985 in Berlin Copyright notice Chapters 2 to 4 have been published in international journals. Only the publishers and the authors have the right for publishing and using the presented data. Any re-use of the presented data requires permissions from the publishers and the authors. Content III Content Summary .................................................................................................................. IV Zusammenfassung .................................................................................................. VI Abbreviations ......................................................................................................... VIII 1 General introduction ............................................................................................. 1 1.1 Importance of fungal diseases of wood and the knowledge about the associated fungal diversity ...................................................................................... 1 1.2 Host-fungus interactions in wood and wood diseases ....................................... 2 1.3 The genus Prunus ............................................................................................. 4 1.4 Diseases and fungal communities of Prunus wood ..........................................
    [Show full text]
  • Supplement Hoenigl TLID 2021 Global Guideline for the Diagnosis
    Supplementary appendix This appendix formed part of the original submission and has been peer reviewed. We post it as supplied by the authors. Supplement to: Hoenigl M, Salmanton-García J, Walsh TJ, et al. Global guideline for the diagnosis and management of rare mould infections: an initiative of the European Confederation of Medical Mycology in cooperation with the International Society for Human and Animal Mycology and the American Society for Microbiology. Lancet Infect Dis 2021; published online Feb 16. https://doi.org/10.1016/S1473-3099(20)30784-2. 1 Global guideline for the diagnosis and management of rare 2 mold infections: An initiative of the ECMM in cooperation 3 with ISHAM and ASM* 4 5 Authors 6 Martin Hoenigl (FECMM)1,2,3,54,55#, Jon Salmanton-García4,5,30,55, Thomas J. Walsh (FECMM)6, Marcio 7 Nucci (FECMM)7, Chin Fen Neoh (FECMM)8,9, Jeffrey D. Jenks2,3,10, Michaela Lackner (FECMM)11,55, Ro- 8 sanne Sprute4,5,55, Abdullah MS Al-Hatmi (FECMM)12, Matteo Bassetti13, Fabianne Carlesse 9 (FECMM)14,15, Tomas Freiberger16, Philipp Koehler (FECMM)4,5,17,30,55, Thomas Lehrnbecher18, Anil Ku- 10 mar (FECMM)19, Juergen Prattes (FECMM)1,55, Malcolm Richardson (FECMM)20,21,55,, Sanjay Revankar 11 (FECMM)22, Monica A. Slavin23,24, Jannik Stemler4,5,55, Birgit Spiess25, Saad J. Taj-Aldeen26, Adilia Warris 12 (FECMM)27, Patrick C.Y. Woo (FECMM)28, Jo-Anne H. Young29, Kerstin Albus4,30,55, Dorothee Arenz4,30,55, 13 Valentina Arsic-Arsenijevic (FECMM)31,54, Jean-Philippe Bouchara32,33, Terrence Rohan Chinniah34, Anu- 14 radha Chowdhary (FECMM)35, G Sybren de Hoog (FECMM)36, George Dimopoulos (FECMM)37, Rafael F.
    [Show full text]
  • Chaetomium Atrobrunneum Causing Human Eumycetoma: the First Report
    SYMPOSIUM Chaetomium atrobrunneum causing human eumycetoma: The first report Najwa A. Mhmoud1,2, Antonella Santona3, Maura Fiamma3, Emmanuel Edwar Siddig1, 3 1,4 3 Massimo Deligios , Sahar Mubarak BakhietID , Salvatore Rubino , Ahmed 1 Hassan FahalID * 1 Mycetoma Research Centre, University of Khartoum, Khartoum, Sudan, 2 Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan, 3 Department of Biomedical Sciences, University of Sassari, Sassari, Italy, 4 Institute for Endemic Diseases, University of Khartoum, Khartoum, Sudan * [email protected], [email protected] Author summary In this communication, a case of black grain eumycetoma produced by the fungus C. atro- a1111111111 brunneum is reported. The patient was initially misdiagnosed with M. mycetomatis eumy- a1111111111 cetoma based on the grains' morphological and cytological features. However, further a1111111111 aerobic culture of the black grains generated a melanised fungus identified as C. atrobrun- a1111111111 neum by conventional morphological methods and by internal transcribed spacer 2 a1111111111 (ITS2) ribosomal RNA gene sequencing. This is the first-ever report of C. atrobrunneum as a eumycetoma-causative organism of black grain eumycetoma. It is essential that the causative organism is identified to the species level, as this is important for proper patient management and to predict treatment outcome and prognosis. OPEN ACCESS Citation: Mhmoud NA, Santona A, Fiamma M, Siddig EE, Deligios M, Bakhiet SM, et al. (2019) Chaetomium atrobrunneum causing human Overview eumycetoma: The first report. PLoS Negl Trop Dis Mycetoma is a chronic, progressive, granulomatous, subcutaneous inflammatory disease. It is 13(5): e0007276. https://doi.org/10.1371/journal. pntd.0007276 caused by certain fungi and bacteria, and thus, it is classified as a eumycetoma and an actino- mycetoma, respectively.
    [Show full text]
  • The Protean Acremonium. A. Sclerotigenum/Egyptiacum: Revision, Food Contaminant, and Human Disease
    microorganisms Article The Protean Acremonium. A. sclerotigenum/egyptiacum: Revision, Food Contaminant, and Human Disease Richard C. Summerbell 1,2,*, Cecile Gueidan 3, Josep Guarro 4, Akif Eskalen 5, Pedro W. Crous 6, Aditya K. Gupta 7,8, Josepa Gené 4, Jose F. Cano-Lira 4, Arien van Iperen 6, Mieke Starink 6 and James A. Scott 2 ID 1 Sporometrics, 219 Dufferin St. Ste. 20C, Toronto, ON M6K 1Y9 Canada 2 Dalla Lana School of Public Health, University of Toronto, Toronto, ON M5T 3M7, Canada; [email protected] 3 Australian National Herbarium, National Research Collections Australia, CSIRO-NCMI, Canberra, ACT 2601, Australia; [email protected] 4 Unitat de Micologia, Facultat de Medicina i Ciencies de la Salut and IISPV, Universitat Rovira i Virgili, Reus, 43201 Tarragona, Spain; [email protected] (J.G.); [email protected] (J.G.); [email protected] (J.F.C.-L.) 5 Department of Plant Pathology, University of California Davis, Davis, CA 95616, USA; [email protected] 6 Westerdijk Fungal Biodiversity Institute, P.O. Box 85167, 3508 AD Utrecht, The Netherlands; [email protected] (P.W.C.); [email protected] (A.v.I.); [email protected] (M.S.) 7 Division of Dermatology, Department of Medicine, University of Toronto, Toronto, ON M5G 2C4, Canada; [email protected] 8 Mediprobe Research Inc., London, ON N5X 2P1, Canada * Correspondence: [email protected]; Tel.: +1-416-516-1660 Received: 2 June 2018; Accepted: 13 August 2018; Published: 16 August 2018 Abstract: Acremonium is known to be regularly isolated from food and also to be a cause of human disease.
    [Show full text]
  • First Report of Six Sordariomycetes Fungi Isolated from Plant Litter in Freshwater Ecosystems of Korea
    The Korean Journal of Mycology www.kjmycology.or.kr RESEARCH ARTICLE First report of six Sordariomycetes fungi isolated from plant litter in freshwater ecosystems of Korea * Jaeduk Goh , Hye Yeon Mun, Yu-Jeong Jeon, Namil Chung, Young-Hwan Park, Sangkyu Park, Hyejin Hwang and Wonsu Cheon Fungi Research Team, Nakdonggang National Institute of Biological Resources, Sangju 37242, Korea * Corresponding author: [email protected] ABSTRACT Freshwater ecosystems provide a complex environment for microorganisms. In this study, we isolated diverse fungal strains from plant litter in freshwaters. These strains were identified using molecular phylogenetic analyses of rDNA and/or other gene sequences (TUB, GAPDH, and EF1). In addition, we examined their morphological characteristics by microscopy and cultural characteristics on several media. We identified six previously unrecorded Sordariomycetes species in Korea, i.e., Colletotrichum godetiae, Discosia rubi, Robillarda sessilis, Monochaetia dimorphospora, Idriella lunata, and Phialemoniopsis endophytica. Of these, D. rubi and M. dimorphospora exhibited high extracellular amylase, lipase, and protease activities, suggesting that these fungal isolates might play an important role as decomposers in freshwater ecosystems. Plant litter could thus be a good source for isolating and investigating previously undocumented fungal species in freshwater environments. Keywords: Freshwater, Plant litter, Sordariomycetes OPEN ACCESS pISSN : 0253-651X eISSN : 2383-5249 Kor. J. Mycol. 2020 June, 48(2): 103-116 INTRODUCTION https://doi.org/10.4489/KJM.20200012 Freshwater ecosystems encompass several habitat types, providing a diverse environment for microorganisms. Plant litter, such as fallen leaves and decaying wood, are typical habitats for several freshwater fungi. Freshwater fungi, which represent a poly-phylogenetic group, are taxonomically diverse Received: April 13, 2020 Revised: June 13, 2020 organisms colonizing the substrate in aquatic or semi-aquatic environments [1-2].
    [Show full text]